Rh1CG412900

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0005221
Physical Location & Seq
Forward (+)
67542305 .. 67545680
3376 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG412900.1

Sequence Viewer

Length: 1524 bp
ATGAATTTTCAGCAAGAGAAGATAGTCAGGTTTCAGGATTGGAATCCAGAGAAACCTATTGATCCACCATATTCTCCAAATGATGGAATGCAGCGAGGAAATTTCAGAACAACATTTAGCTCGGTCTCATCTAAATTTCAAAGACGCCTGGAATCTGGTTCTGAGAGGATTAAGAAGTCATGGAAGTCCTATTCGATTGATGGGGTTGTTGGTAAAAGCTTTTGTTCTAGAGTTCTTGATCCGCAAGGGCAATTCCTTCAAAAGTGGAATAAGATATTTGTATTGGCATGTGTAATTGCTGTCTCCCTGGATCCTTTGTTCTTTTACGTCCCTGTGATTGATGATGACAATAAGTGCCTTGACAAGGACAGAAAGATGGAGATAACAGCTAGTGTTTTACGCTCATTCACCGACATATTTTATGTAGTTCATATAATTTTTCAATTTCGTACTGGATTTATTGCTCCTCCTTCTCGAGTTTTTGGAAGAGGTGTTTTAGTTGAAGATGCTTGGGCTATTGCAAAGAGGTATCTATCCTCATACTTCCTCATTGACATTCTTGCTGTCCTTCCTCTCCCACAGACATATTTGCAGTCTACAACTACAAGATTGGAGGAGATGAGAGTAAAAAGGAGAGATGCAGAACAATGGATGTCCCACCGTTTGCTACCAGAGAATTTGAGAGAACGCATAAGGCGGTATGAACAATACAGATGGCAAGAAACCAGAGGTGTAGATGAAGAGAATTTGATCTGTAATCTTCCGAAGGACCTTAGAAGGGACATAAAGCGCCATCTCTGCTTGGCTCTACTAATGAGGGTGCCAATGTTTGAGAAAATGGATGACCAATTGTTGGATGCAATGTGTGACCGTCTCAAGCCAGTACTGTATACAGAAGACAGCTACATTGTTCGGGAGGGAGACCCAGTGGATGAGATGCTCTTCATAATGCGAGGCAAGCTATTGACTATGACCACAAATGGTGGAAGGACTGGGTTCTTCAATTCTGAATATCTCAAGGCCGGTGACTTCTGTGGTGAAGAGCTTCTCACATGGGCTCTTGATCCTCACACCTCTCCTAATCTTCCCATCTCAACTAGAACTGTCCAAGCCCTTAGTGAAGTCGAAGCCTTTGCTTTGAAAGCAGATGATCTGAAGTTTGTAGCCTCCCAGTTTCGACGGCTTCACAGCAAGCAGCTCCGCCACACATTCAGGTTTTACTCACAACAGTGGAGGACCTGGGCAGCTTGCTTTATACAGGCAGCATGGCGCCGTTATAGTAAGAAGAAGCTTGAAGAGTCTCTGCGGGAAGAGGAGAATAGGTTGCAGGATGCACTTGCAAAGGCCGAGACTAGCTCCCCCAGTCTTGGTGCCACCATTTATGCCTCGCGATTCGCTGCTAATGTACTTCGCGCTGTACGGCTACGGCGCAGTGGCACACGCAAGGGAAGGGTGCCAGAGAGAATACCGCCCATGCTACTTCAGAAGCCAGCAGAGCCGGATTTTACTGCTGAAGAACAATAG

Protein Analysis

507

Amino Acids

59.24

Weight (kDa)

9.31

Isoelectric Point (pI)

55.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 297 - 388 3.3e-07 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 820, 1269, 1370, 1453
AccB7I CCANNNNNTGG 2 cut(s) 83, 853
AccI GTMKAC 2 cut(s) 596, 890
AccII CGCG 2 cut(s) 1390, 1413
AciI CCGC 5 cut(s) 242, 697, 1201, 1306, 1469
AclWI GGATC 5 cut(s) 56, 233, 305, 318, 1058
AcsI RAATTY 5 cut(s) 4, 100, 134, 676, 745
AcuI CTGAAG 2 cut(s) 1175, 1466
AcyI GRCGYC 2 cut(s) 145, 1270
AfaI GTAC 4 cut(s) 451, 885, 1407, 1419
AfiI CCNNNNNNNGG 5 cut(s) 83, 364, 778, 853, 1367
AgsI TTSAA 7 cut(s) 140, 260, 443, 503, 1003, 1141, 1295
AjnI CCWGG 3 cut(s) 147, 306, 1238
AleI CACNNNNGTG 1 cut(s) 1228
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
Alw26I GTCTC 6 cut(s) 130, 307, 878, 915, 1305, 1343
AlwI GGATC 5 cut(s) 56, 233, 305, 318, 1058
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 2 cut(s) 1020, 1344
ApeKI GCWGC 5 cut(s) 91, 1195, 1244, 1262, 1397
ApoI RAATTY 5 cut(s) 4, 100, 134, 676, 745
Asp700I GAANNNNTTC 1 cut(s) 1044
AspLEI GCGC 4 cut(s) 792, 1272, 1415, 1431
AspS9I GGNCC 2 cut(s) 769, 1236
AsuHPI GGTGA 3 cut(s) 400, 1037, 1049
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 2 cut(s) 769, 1236
BamHI GGATCC 1 cut(s) 310
BanI GGYRCC 4 cut(s) 820, 1269, 1370, 1453
BanII GRGCYC 1 cut(s) 1060
BbsI GAAGAC 1 cut(s) 903
BbvI GCAGC 5 cut(s) 103, 1207, 1256, 1274, 1384
BccI CCATC 6 cut(s) 77, 194, 370, 708, 801, 1097
BceAI ACGGC 4 cut(s) 1196, 1257, 1436, 1442
BcgI CGANNNNNNTGC 2 cut(s) 1115, 1149
BciT130I CCWGG 3 cut(s) 149, 308, 1240
BcoDI GTCTC 6 cut(s) 130, 307, 878, 915, 1305, 1343
BfaI CTAG 4 cut(s) 228, 390, 1098, 1353
BfoI RGCGCY 2 cut(s) 793, 1273
BisI GCNGC 5 cut(s) 92, 1196, 1245, 1263, 1398
BlsI GCNGC 5 cut(s) 93, 1197, 1246, 1264, 1399
BmcAI AGTACT 1 cut(s) 885
Bme1390I CCNGG 3 cut(s) 149, 308, 1240
Bme18I GGWCC 2 cut(s) 769, 1236
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 2 cut(s) 769, 1236
BmiI GGNNCC 5 cut(s) 312, 822, 1271, 1372, 1455
BmrFI CCNGG 3 cut(s) 149, 308, 1240
BmrI ACTGGG 4 cut(s) 920, 1002, 1165, 1356
BmsI GCATC 5 cut(s) 496, 628, 847, 927, 1321
BmuI ACTGGG 4 cut(s) 920, 1002, 1165, 1356
BpiI GAAGAC 1 cut(s) 903
BplI GAGNNNNNCTC 2 cut(s) 1340, 1372
BpuEI CTTGAG 2 cut(s) 860, 1001
BsaBI GATNNNNATC 1 cut(s) 42
BsaHI GRCGYC 2 cut(s) 145, 1270
BsaI GGTCTC 2 cut(s) 130, 915
BsaJI CCNNGG 2 cut(s) 306, 1239
BsaXI ACNNNNNCTCC 4 cut(s) 912, 942, 1307, 1337
Bsc4I CCNNNNNNNGG 5 cut(s) 83, 364, 778, 853, 1367
Bse118I RCCGGY 1 cut(s) 1022
Bse1I ACTGG 6 cut(s) 457, 881, 926, 997, 1171, 1362
Bse3DI GCAATG 1 cut(s) 867
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 3 cut(s) 149, 308, 1240
BseDI CCNNGG 2 cut(s) 306, 1239
BseGI GGATG 5 cut(s) 657, 847, 862, 937, 1336
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 5 cut(s) 83, 364, 778, 853, 1367
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 6 cut(s) 457, 881, 926, 997, 1171, 1362
BseRI GAGGAG 3 cut(s) 456, 629, 1328
BseXI GCAGC 5 cut(s) 103, 1207, 1256, 1274, 1384
Bsh1236I CGCG 2 cut(s) 1390, 1413
BshFI GGCC 2 cut(s) 1022, 1346
BshNI GGYRCC 4 cut(s) 820, 1269, 1370, 1453
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 2 cut(s) 1023, 1499
BslFI GGGAC 3 cut(s) 314, 640, 794
BslI CCNNNNNNNGG 5 cut(s) 83, 364, 778, 853, 1367
BsmAI GTCTC 6 cut(s) 130, 307, 878, 915, 1305, 1343
BsmBI CGTCTC 1 cut(s) 878
BsmFI GGGAC 3 cut(s) 314, 640, 794
BsmI GAATGC 1 cut(s) 93
BsnI GGCC 2 cut(s) 1022, 1346
Bso31I GGTCTC 2 cut(s) 130, 915
BsoBI CYCGRG 1 cut(s) 474
Bsp1286I GDGCHC 1 cut(s) 1060
Bsp143I GATC 6 cut(s) 61, 238, 310, 750, 1063, 1150
Bsp68I TCGCGA 1 cut(s) 1390
BspACI CCGC 5 cut(s) 242, 697, 1201, 1306, 1469
BspANI GGCC 2 cut(s) 1022, 1346
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 2 cut(s) 1390, 1413
BspLI GGNNCC 5 cut(s) 312, 822, 1271, 1372, 1455
BspPI GGATC 5 cut(s) 56, 233, 305, 318, 1058
BspQI GCTCTTC 2 cut(s) 947, 1035
BspT107I GGYRCC 4 cut(s) 820, 1269, 1370, 1453
BspTNI GGTCTC 2 cut(s) 130, 915
BsrDI GCAATG 1 cut(s) 867
BsrFI RCCGGY 1 cut(s) 1022
BsrI ACTGG 6 cut(s) 457, 881, 926, 997, 1171, 1362
BssAI RCCGGY 1 cut(s) 1022
BssECI CCNNGG 2 cut(s) 306, 1239
BssMI GATC 6 cut(s) 61, 238, 310, 750, 1063, 1150
BssNAI GTATAC 1 cut(s) 891
BssNI GRCGYC 2 cut(s) 145, 1270
Bst1107I GTATAC 1 cut(s) 891
Bst2UI CCWGG 3 cut(s) 149, 308, 1240
Bst4CI ACNGT 5 cut(s) 662, 872, 888, 1105, 1230
Bst6I CTCTTC 6 cut(s) 481, 735, 947, 1035, 1290, 1305
BstACI GRCGYC 2 cut(s) 145, 1270
BstC8I GCNNGC 4 cut(s) 959, 1193, 1249, 1491
BstDEI CTNAG 3 cut(s) 162, 773, 1115
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 5 cut(s) 657, 847, 862, 937, 1336
BstFNI CGCG 2 cut(s) 1390, 1413
BstH2I RGCGCY 2 cut(s) 793, 1273
BstHHI GCGC 4 cut(s) 792, 1272, 1415, 1431
BstKTI GATC 6 cut(s) 64, 241, 313, 753, 1066, 1153
BstMAI GTCTC 6 cut(s) 130, 307, 878, 915, 1305, 1343
BstMBI GATC 6 cut(s) 61, 238, 310, 750, 1063, 1150
BstMWI GCNNNNNNNGC 4 cut(s) 798, 958, 1142, 1495
BstNI CCWGG 3 cut(s) 149, 308, 1240
BstNSI RCATGY 1 cut(s) 291
BstSCI CCNGG 3 cut(s) 147, 306, 1238
BstUI CGCG 2 cut(s) 1390, 1413
BstV1I GCAGC 5 cut(s) 103, 1207, 1256, 1274, 1384
BstV2I GAAGAC 1 cut(s) 903
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BstZ17I GTATAC 1 cut(s) 891
BsuRI GGCC 2 cut(s) 1022, 1346
BtsCI GGATG 5 cut(s) 657, 847, 862, 937, 1336
BtsI GCAGTG 1 cut(s) 1438
BtsIMutI CAGTG 3 cut(s) 933, 1235, 1438
BtuMI TCGCGA 1 cut(s) 1390
Cac8I GCNNGC 4 cut(s) 959, 1193, 1249, 1491
CfoI GCGC 4 cut(s) 792, 1272, 1415, 1431
Cfr10I RCCGGY 1 cut(s) 1022
Cfr13I GGNCC 2 cut(s) 769, 1236
CseI GACGC 1 cut(s) 153
Csp6I GTAC 4 cut(s) 450, 884, 1406, 1418
CviAII CATG 5 cut(s) 180, 288, 1053, 1266, 1474
CviQI GTAC 4 cut(s) 450, 884, 1406, 1418
DdeI CTNAG 3 cut(s) 162, 773, 1115
DinI GGCGCC 1 cut(s) 1271
DpnI GATC 6 cut(s) 63, 240, 312, 752, 1065, 1152
DpnII GATC 6 cut(s) 61, 238, 310, 750, 1063, 1150
Eam1104I CTCTTC 6 cut(s) 481, 735, 947, 1035, 1290, 1305
EarI CTCTTC 6 cut(s) 481, 735, 947, 1035, 1290, 1305
EciI GGCGGA 1 cut(s) 1190
Eco24I GRGCYC 1 cut(s) 1060
Eco31I GGTCTC 2 cut(s) 130, 915
Eco47I GGWCC 2 cut(s) 769, 1236
Eco57I CTGAAG 2 cut(s) 1175, 1466
Eco88I CYCGRG 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoO109I RGGNCCY 2 cut(s) 769, 1236
EcoRII CCWGG 3 cut(s) 147, 306, 1238
EcoT38I GRGCYC 1 cut(s) 1060
EgeI GGCGCC 1 cut(s) 1271
EheI GGCGCC 1 cut(s) 1271
Esp3I CGTCTC 1 cut(s) 878
FaeI CATG 5 cut(s) 183, 291, 1056, 1269, 1477
FaqI GGGAC 3 cut(s) 314, 640, 794
FatI CATG 5 cut(s) 179, 287, 1052, 1265, 1473
FauI CCCGC 1 cut(s) 1299
FblI GTMKAC 2 cut(s) 596, 890
Fnu4HI GCNGC 5 cut(s) 92, 1196, 1245, 1263, 1398
FokI GGATG 5 cut(s) 664, 854, 869, 944, 1343
FriOI GRGCYC 1 cut(s) 1060
Fsp4HI GCNGC 5 cut(s) 92, 1196, 1245, 1263, 1398
FspBI CTAG 4 cut(s) 228, 390, 1098, 1353
GlaI GCGC 4 cut(s) 791, 1271, 1414, 1430
GluI GCNGC 5 cut(s) 92, 1196, 1245, 1263, 1398
HaeII RGCGCY 2 cut(s) 793, 1273
HaeIII GGCC 2 cut(s) 1022, 1346
HapII CCGG 2 cut(s) 1023, 1499
HgaI GACGC 1 cut(s) 153
HhaI GCGC 4 cut(s) 792, 1272, 1415, 1431
Hin1I GRCGYC 2 cut(s) 145, 1270
Hin1II CATG 5 cut(s) 183, 291, 1056, 1269, 1477
Hin6I GCGC 4 cut(s) 790, 1270, 1413, 1429
HinP1I GCGC 4 cut(s) 790, 1270, 1413, 1429
HindIII AAGCTT 2 cut(s) 217, 1289
HinfI GANTC 4 cut(s) 43, 152, 1298, 1392
HpaII CCGG 2 cut(s) 1023, 1499
HphI GGTGA 3 cut(s) 400, 1037, 1049
Hpy166II GTNNAC 2 cut(s) 597, 891
Hpy188I TCNGA 6 cut(s) 107, 163, 765, 1009, 1155, 1485
Hpy188III TCNNGA 8 cut(s) 35, 47, 228, 236, 474, 914, 1061, 1389
Hpy8I GTNNAC 2 cut(s) 597, 891
Hpy99I CGWCG 1 cut(s) 1182
HpyAV CCTTC 7 cut(s) 266, 480, 578, 760, 771, 981, 1443
HpyCH4III ACNGT 5 cut(s) 662, 872, 888, 1105, 1230
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 8 cut(s) 91, 521, 592, 641, 860, 1327, 1334, 1340
HpyF10VI GCNNNNNNNGC 4 cut(s) 798, 958, 1142, 1495
HpyF3I CTNAG 3 cut(s) 162, 773, 1115
HpySE526I ACGT 1 cut(s) 327
Hsp92I GRCGYC 2 cut(s) 145, 1270
Hsp92II CATG 5 cut(s) 183, 291, 1056, 1269, 1477
HspAI GCGC 4 cut(s) 790, 1270, 1413, 1429
KasI GGCGCC 1 cut(s) 1269
Kzo9I GATC 6 cut(s) 61, 238, 310, 750, 1063, 1150
LguI GCTCTTC 2 cut(s) 947, 1035
LmnI GCTCC 3 cut(s) 469, 1203, 1361
Lsp1109I GCAGC 5 cut(s) 103, 1207, 1256, 1274, 1384
LweI GCATC 5 cut(s) 496, 628, 847, 927, 1321
MaeI CTAG 4 cut(s) 228, 390, 1098, 1353
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 866, 1025
MalI GATC 6 cut(s) 63, 240, 312, 752, 1065, 1152
MboI GATC 6 cut(s) 61, 238, 310, 750, 1063, 1150
MfeI CAATTG 1 cut(s) 848
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 1 cut(s) 1060
Mly113I GGCGCC 1 cut(s) 1270
MlyI GAGTC 1 cut(s) 1307
MmeI TCCRAC 1 cut(s) 834
MroXI GAANNNNTTC 1 cut(s) 1044
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 1 cut(s) 1228
MspI CCGG 2 cut(s) 1023, 1499
MspR9I CCNGG 3 cut(s) 149, 308, 1240
MunI CAATTG 1 cut(s) 848
Mva1269I GAATGC 1 cut(s) 93
MvaI CCWGG 3 cut(s) 149, 308, 1240
MvnI CGCG 2 cut(s) 1390, 1413
MwoI GCNNNNNNNGC 4 cut(s) 798, 958, 1142, 1495
NarI GGCGCC 1 cut(s) 1270
NdeII GATC 6 cut(s) 61, 238, 310, 750, 1063, 1150
NlaIII CATG 5 cut(s) 183, 291, 1056, 1269, 1477
NlaIV GGNNCC 5 cut(s) 312, 822, 1271, 1372, 1455
NmeAIII GCCGAG 1 cut(s) 1372
NmuCI GTSAC 2 cut(s) 866, 1025
NruI TCGCGA 1 cut(s) 1390
NspI RCATGY 1 cut(s) 291
OliI CACNNNNGTG 1 cut(s) 1228
PaeR7I CTCGAG 1 cut(s) 474
PciSI GCTCTTC 2 cut(s) 947, 1035
PctI GAATGC 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 1044
PfeI GAWTC 3 cut(s) 43, 152, 1392
PflMI CCANNNNNTGG 2 cut(s) 83, 853
PkrI GCNGC 5 cut(s) 93, 1197, 1246, 1264, 1399
PleI GAGTC 1 cut(s) 1306
PluTI GGCGCC 1 cut(s) 1273
PpsI GAGTC 1 cut(s) 1306
PpuMI RGGWCCY 2 cut(s) 769, 1236
Psp5II RGGWCCY 2 cut(s) 769, 1236
Psp6I CCWGG 3 cut(s) 147, 306, 1238
PspGI CCWGG 3 cut(s) 147, 306, 1238
PspN4I GGNNCC 5 cut(s) 312, 822, 1271, 1372, 1455
PspPI GGNCC 2 cut(s) 769, 1236
PspPPI RGGWCCY 2 cut(s) 769, 1236
PsuI RGATCY 1 cut(s) 310
RruI TCGCGA 1 cut(s) 1390
RsaI GTAC 4 cut(s) 451, 885, 1407, 1419
RsaNI GTAC 4 cut(s) 450, 884, 1406, 1418
RseI CAYNNNNRTG 1 cut(s) 1228
SapI GCTCTTC 2 cut(s) 947, 1035
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 5 cut(s) 92, 1196, 1245, 1263, 1398
Sau3AI GATC 6 cut(s) 61, 238, 310, 750, 1063, 1150
Sau96I GGNCC 2 cut(s) 769, 1236
ScaI AGTACT 1 cut(s) 885
SchI GAGTC 1 cut(s) 1307
ScrFI CCNGG 3 cut(s) 149, 308, 1240
SduI GDGCHC 1 cut(s) 1060
SfaNI GCATC 5 cut(s) 496, 628, 847, 927, 1321
SfoI GGCGCC 1 cut(s) 1271
Sfr274I CTCGAG 1 cut(s) 474
SinI GGWCC 2 cut(s) 769, 1236
SlaI CTCGAG 1 cut(s) 474
SmiMI CAYNNNNRTG 1 cut(s) 1228
SmlI CTYRAG 3 cut(s) 474, 875, 1016
SmoI CTYRAG 3 cut(s) 474, 875, 1016
SsiI CCGC 5 cut(s) 242, 697, 1201, 1306, 1469
SspDI GGCGCC 1 cut(s) 1269
SspMI CTAG 4 cut(s) 228, 390, 1098, 1353
StyD4I CCNGG 3 cut(s) 147, 306, 1238
TaaI ACNGT 5 cut(s) 662, 872, 888, 1105, 1230
TaiI ACGT 1 cut(s) 330
TaqI TCGA 4 cut(s) 194, 475, 1125, 1177
TaqII GACCGA 1 cut(s) 112
TatI WGTACW 2 cut(s) 883, 1405
TfiI GAWTC 3 cut(s) 43, 152, 1392
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 3 cut(s) 933, 1235, 1438
TseFI GTSAC 2 cut(s) 866, 1025
TseI GCWGC 5 cut(s) 91, 1195, 1244, 1262, 1397
Tsp45I GTSAC 2 cut(s) 866, 1025
TspDTI ATGAA 5 cut(s) 17, 419, 717, 753, 934
TspRI CASTG 3 cut(s) 933, 1235, 1438
Van91I CCANNNNNTGG 2 cut(s) 83, 853
VpaK11BI GGWCC 2 cut(s) 769, 1236
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 5 cut(s) 4, 100, 134, 676, 745
XbaI TCTAGA 1 cut(s) 227
XceI RCATGY 1 cut(s) 291
XhoI CTCGAG 1 cut(s) 474
XmiI GTMKAC 2 cut(s) 596, 890
XmnI GAANNNNTTC 1 cut(s) 1044
XspI CTAG 4 cut(s) 228, 390, 1098, 1353
ZrmI AGTACT 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.