RchiOBHm_Chr1g0380301

Cyclic nucleotide-gated ion channel 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005221
Physical Location & Seq
Forward (+)
65993122 .. 65994076
955 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ60359

Sequence Viewer

Length: 864 bp
ATGACAGCCACCGTCGCCGGAAAACCAGCTGAAGGTACTCTCCAGCAACACAGATGGCAAGAAACCAGAGGTGTAGATGAAGAGAATTTGATCTGTAATCTTCCGAAGGACCTTAGAAGGGACATAAAGCGCCATCTCTGCTTGGCTCTACTAATGAGGGTGCCAATGTTTGAGAAAATGGATGACCAATTGTTGGATGCAATGTGTGACCGTCTCAAGCCAGTACTGTATACTGAAGACAGCTACATTGTTCGGGAGGGAGACCCAGTGGATGAAATGCTCTTCATAATGCGAGGCAAGCTATTGACTATGACCACAAATGGTGGAAGGACTGGGTTCTTCAATTCTGAATATCTCAAGGCCGGTGATTTCTGTGGTGAAGAGCTTCTCACATGGGCTCTTGATCCTCACACCTCTCCTAATCTTCCCATCTCAACTAGAACTGTCCAAGCCCTTAGTGAAGTCGAAGCCTTTGCTTTGAAAGCAGACGATCTGAAGTTTGTAGCCTCCCAATTTCGACGGCTTCACAGCAAGCAGCTCCGCCACACATTCAGGTTTTACTCTCAACAGTGGAGGACTTGGGCGGCTTGCTTTATTCAGGCAGCATGGCGTCGTTATAGTAAGAAGAAGCTTGAAGAGTCTCTGCGGGAAGAGGAGAATAGGTTGCAAGATGCACTTGCGAAGGCAGAGACAAGCTCCCCTAGTCTTGGTGCCACCATTTATGCCTCGCGATTCGCTGCTAATGTACTTCGCGCTGTACGGCTGCGGCGCAGTGGTACACGCAAGGGAAGGGTGCCAGAGAGAATACCGCCCATGCTACTTCAGAAGCCAGCAGAGCCGGATTTTACTGCTGAAGAACAATAG

Protein Analysis

287

Amino Acids

33.04

Weight (kDa)

8.93

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 77 - 168 1.2e-07 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 160, 710, 793
AccB7I CCANNNNNTGG 1 cut(s) 193
AccI GTMKAC 1 cut(s) 230
AccII CGCG 2 cut(s) 730, 753
AciI CCGC 5 cut(s) 541, 584, 646, 766, 809
AclWI GGATC 1 cut(s) 398
AcsI RAATTY 1 cut(s) 85
AcuI CTGAAG 4 cut(s) 51, 255, 515, 806
AcyI GRCGYC 1 cut(s) 610
AfaI GTAC 5 cut(s) 37, 225, 747, 759, 778
AfiI CCNNNNNNNGG 4 cut(s) 32, 118, 193, 707
AgsI TTSAA 3 cut(s) 343, 481, 635
AluBI AGCT 7 cut(s) 29, 243, 301, 385, 538, 631, 696
AluI AGCT 7 cut(s) 29, 243, 301, 385, 538, 631, 696
Alw26I GTCTC 4 cut(s) 218, 255, 645, 683
AlwI GGATC 1 cut(s) 398
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 4 cut(s) 535, 602, 737, 763
ApoI RAATTY 1 cut(s) 85
Asp700I GAANNNNTTC 1 cut(s) 384
AspLEI GCGC 3 cut(s) 132, 755, 771
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 377, 389
AvaII GGWCC 1 cut(s) 109
BanI GGYRCC 3 cut(s) 160, 710, 793
BanII GRGCYC 1 cut(s) 400
BbsI GAAGAC 1 cut(s) 243
BbvI GCAGC 4 cut(s) 547, 614, 724, 750
BccI CCATC 3 cut(s) 48, 141, 437
BceAI ACGGC 2 cut(s) 536, 776
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BcoDI GTCTC 4 cut(s) 218, 255, 645, 683
BfaI CTAG 2 cut(s) 438, 702
BfoI RGCGCY 1 cut(s) 133
BisI GCNGC 6 cut(s) 536, 585, 603, 738, 764, 767
BlsI GCNGC 6 cut(s) 537, 586, 604, 739, 765, 768
BmcAI AGTACT 1 cut(s) 225
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 3 cut(s) 162, 712, 795
BmrI ACTGGG 2 cut(s) 260, 342
BmsI GCATC 2 cut(s) 187, 661
BmuI ACTGGG 2 cut(s) 260, 342
BpiI GAAGAC 1 cut(s) 243
BplI GAGNNNNNCTC 2 cut(s) 680, 712
BpmI CTGGAG 1 cut(s) 26
BpuEI CTTGAG 2 cut(s) 200, 341
BsaHI GRCGYC 1 cut(s) 610
BsaI GGTCTC 1 cut(s) 255
BsaXI ACNNNNNCTCC 4 cut(s) 252, 282, 647, 677
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 118, 193, 707
Bse118I RCCGGY 1 cut(s) 362
Bse1I ACTGG 3 cut(s) 221, 266, 337
Bse3DI GCAATG 1 cut(s) 207
BseGI GGATG 3 cut(s) 187, 202, 277
BseLI CCNNNNNNNGG 4 cut(s) 32, 118, 193, 707
BseMI GCAATG 1 cut(s) 207
BseNI ACTGG 3 cut(s) 221, 266, 337
BseRI GAGGAG 1 cut(s) 668
BseXI GCAGC 4 cut(s) 547, 614, 724, 750
Bsh1236I CGCG 2 cut(s) 730, 753
BshFI GGCC 1 cut(s) 362
BshNI GGYRCC 3 cut(s) 160, 710, 793
BsiSI CCGG 3 cut(s) 18, 363, 839
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 4 cut(s) 32, 118, 193, 707
BsmAI GTCTC 4 cut(s) 218, 255, 645, 683
BsmBI CGTCTC 1 cut(s) 218
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 1 cut(s) 362
Bso31I GGTCTC 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 400
Bsp143I GATC 3 cut(s) 90, 403, 490
Bsp68I TCGCGA 1 cut(s) 730
BspACI CCGC 5 cut(s) 541, 584, 646, 766, 809
BspANI GGCC 1 cut(s) 362
BspFNI CGCG 2 cut(s) 730, 753
BspLI GGNNCC 3 cut(s) 162, 712, 795
BspPI GGATC 1 cut(s) 398
BspQI GCTCTTC 2 cut(s) 287, 375
BspT107I GGYRCC 3 cut(s) 160, 710, 793
BspTNI GGTCTC 1 cut(s) 255
BsrDI GCAATG 1 cut(s) 207
BsrFI RCCGGY 1 cut(s) 362
BsrI ACTGG 3 cut(s) 221, 266, 337
BssAI RCCGGY 1 cut(s) 362
BssMI GATC 3 cut(s) 90, 403, 490
BssNAI GTATAC 1 cut(s) 231
BssNI GRCGYC 1 cut(s) 610
Bst1107I GTATAC 1 cut(s) 231
Bst4CI ACNGT 5 cut(s) 13, 212, 228, 445, 570
Bst6I CTCTTC 5 cut(s) 75, 287, 375, 630, 645
BstACI GRCGYC 1 cut(s) 610
BstC8I GCNNGC 4 cut(s) 299, 533, 589, 831
BstDEI CTNAG 2 cut(s) 113, 455
BstF5I GGATG 3 cut(s) 187, 202, 277
BstFNI CGCG 2 cut(s) 730, 753
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 3 cut(s) 132, 755, 771
BstKTI GATC 3 cut(s) 93, 406, 493
BstMAI GTCTC 4 cut(s) 218, 255, 645, 683
BstMBI GATC 3 cut(s) 90, 403, 490
BstMWI GCNNNNNNNGC 5 cut(s) 14, 138, 298, 482, 835
BstUI CGCG 2 cut(s) 730, 753
BstV1I GCAGC 4 cut(s) 547, 614, 724, 750
BstV2I GAAGAC 1 cut(s) 243
BstZ17I GTATAC 1 cut(s) 231
BsuRI GGCC 1 cut(s) 362
BtsCI GGATG 3 cut(s) 187, 202, 277
BtsI GCAGTG 1 cut(s) 778
BtsIMutI CAGTG 3 cut(s) 273, 575, 778
BtuMI TCGCGA 1 cut(s) 730
Cac8I GCNNGC 4 cut(s) 299, 533, 589, 831
CfoI GCGC 3 cut(s) 132, 755, 771
Cfr10I RCCGGY 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 1 cut(s) 599
Csp6I GTAC 5 cut(s) 36, 224, 746, 758, 777
CviAII CATG 3 cut(s) 393, 606, 814
CviQI GTAC 5 cut(s) 36, 224, 746, 758, 777
DdeI CTNAG 2 cut(s) 113, 455
DpnI GATC 3 cut(s) 92, 405, 492
DpnII GATC 3 cut(s) 90, 403, 490
Eam1104I CTCTTC 5 cut(s) 75, 287, 375, 630, 645
EarI CTCTTC 5 cut(s) 75, 287, 375, 630, 645
EciI GGCGGA 1 cut(s) 530
Eco24I GRGCYC 1 cut(s) 400
Eco31I GGTCTC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 109
Eco57I CTGAAG 4 cut(s) 51, 255, 515, 806
EcoO109I RGGNCCY 1 cut(s) 109
EcoT38I GRGCYC 1 cut(s) 400
Esp3I CGTCTC 1 cut(s) 218
FaeI CATG 3 cut(s) 396, 609, 817
FaiI YATR 9 cut(s) 125, 231, 287, 311, 394, 607, 618, 723, 815
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 1 cut(s) 134
FatI CATG 3 cut(s) 392, 605, 813
FauI CCCGC 1 cut(s) 639
FblI GTMKAC 1 cut(s) 230
Fnu4HI GCNGC 6 cut(s) 536, 585, 603, 738, 764, 767
FokI GGATG 3 cut(s) 194, 209, 284
FriOI GRGCYC 1 cut(s) 400
Fsp4HI GCNGC 6 cut(s) 536, 585, 603, 738, 764, 767
FspBI CTAG 2 cut(s) 438, 702
GlaI GCGC 3 cut(s) 131, 754, 770
GluI GCNGC 6 cut(s) 536, 585, 603, 738, 764, 767
GsuI CTGGAG 1 cut(s) 26
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 1 cut(s) 362
HapII CCGG 3 cut(s) 18, 363, 839
HgaI GACGC 1 cut(s) 599
HhaI GCGC 3 cut(s) 132, 755, 771
Hin1I GRCGYC 1 cut(s) 610
Hin1II CATG 3 cut(s) 396, 609, 817
Hin6I GCGC 3 cut(s) 130, 753, 769
HinP1I GCGC 3 cut(s) 130, 753, 769
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 2 cut(s) 638, 732
HpaII CCGG 3 cut(s) 18, 363, 839
HphI GGTGA 2 cut(s) 377, 389
Hpy166II GTNNAC 2 cut(s) 231, 779
Hpy188I TCNGA 4 cut(s) 105, 349, 495, 825
Hpy188III TCNNGA 3 cut(s) 254, 401, 729
Hpy8I GTNNAC 2 cut(s) 231, 779
Hpy99I CGWCG 3 cut(s) 17, 522, 615
HpyAV CCTTC 6 cut(s) 26, 100, 111, 321, 676, 783
HpyCH4III ACNGT 5 cut(s) 13, 212, 228, 445, 570
HpyCH4V TGCA 3 cut(s) 200, 667, 674
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 138, 298, 482, 835
HpyF3I CTNAG 2 cut(s) 113, 455
Hsp92I GRCGYC 1 cut(s) 610
Hsp92II CATG 3 cut(s) 396, 609, 817
HspAI GCGC 3 cut(s) 130, 753, 769
Kzo9I GATC 3 cut(s) 90, 403, 490
LguI GCTCTTC 2 cut(s) 287, 375
LmnI GCTCC 2 cut(s) 543, 701
Lsp1109I GCAGC 4 cut(s) 547, 614, 724, 750
LweI GCATC 2 cut(s) 187, 661
MaeI CTAG 2 cut(s) 438, 702
MaeIII GTNAC 1 cut(s) 206
MalI GATC 3 cut(s) 92, 405, 492
MboI GATC 3 cut(s) 90, 403, 490
MfeI CAATTG 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 4 cut(s) 85, 188, 343, 512
MlyI GAGTC 1 cut(s) 647
MmeI TCCRAC 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 3 cut(s) 18, 363, 839
MunI CAATTG 1 cut(s) 188
MvnI CGCG 2 cut(s) 730, 753
MwoI GCNNNNNNNGC 5 cut(s) 14, 138, 298, 482, 835
NdeII GATC 3 cut(s) 90, 403, 490
NlaIII CATG 3 cut(s) 396, 609, 817
NlaIV GGNNCC 3 cut(s) 162, 712, 795
NmuCI GTSAC 1 cut(s) 206
NruI TCGCGA 1 cut(s) 730
PciSI GCTCTTC 2 cut(s) 287, 375
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 732
PflMI CCANNNNNTGG 1 cut(s) 193
PkrI GCNGC 6 cut(s) 537, 586, 604, 739, 765, 768
PleI GAGTC 1 cut(s) 646
PpsI GAGTC 1 cut(s) 646
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
PspN4I GGNNCC 3 cut(s) 162, 712, 795
PspPI GGNCC 1 cut(s) 109
PspPPI RGGWCCY 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 29
RruI TCGCGA 1 cut(s) 730
RsaI GTAC 5 cut(s) 37, 225, 747, 759, 778
RsaNI GTAC 5 cut(s) 36, 224, 746, 758, 777
SapI GCTCTTC 2 cut(s) 287, 375
SatI GCNGC 6 cut(s) 536, 585, 603, 738, 764, 767
Sau3AI GATC 3 cut(s) 90, 403, 490
Sau96I GGNCC 1 cut(s) 109
ScaI AGTACT 1 cut(s) 225
SchI GAGTC 1 cut(s) 647
SduI GDGCHC 1 cut(s) 400
SfaNI GCATC 2 cut(s) 187, 661
SinI GGWCC 1 cut(s) 109
SmlI CTYRAG 2 cut(s) 215, 356
SmoI CTYRAG 2 cut(s) 215, 356
Sse9I AATT 4 cut(s) 85, 188, 343, 512
SsiI CCGC 5 cut(s) 541, 584, 646, 766, 809
SspMI CTAG 2 cut(s) 438, 702
TaaI ACNGT 5 cut(s) 13, 212, 228, 445, 570
TaqI TCGA 2 cut(s) 465, 517
TasI AATT 4 cut(s) 85, 188, 343, 512
TatI WGTACW 2 cut(s) 223, 745
TauI GCSGC 2 cut(s) 587, 769
TfiI GAWTC 1 cut(s) 732
TscAI CASTG 3 cut(s) 273, 575, 778
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 4 cut(s) 535, 602, 737, 763
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 3 cut(s) 93, 274, 288
TspRI CASTG 3 cut(s) 273, 575, 778
Van91I CCANNNNNTGG 1 cut(s) 193
VpaK11BI GGWCC 1 cut(s) 109
XapI RAATTY 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 230
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 2 cut(s) 438, 702
ZrmI AGTACT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.