MD02G1021100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014628
Physical Location & Seq
Reverse (-)
1491774 .. 1492925
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1021100.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGGAGGTCAAGCTCCATCAAAAGGAAGACGGCCCACGAGTCCTCAAGGTCCTAGAAGCTCTAAAACAAGCCTCCCACGAGCTACAGACCCACCCTAGTCCCGATTCGCCCGAGTCAAACTCATCCTCCGCCATTAACGCCCTCCTCGAGCTCGAGACCGAGTCCGACAACATCCTCTCCCGGGACCCACAACTCTCCGCCCTCTCCAAACACCTCGCCACCCTCAAAACCCTCGTAGAGTCCCTCCGCCAGTCCAGCGGCCACCTCAGCATCCGCTCCTTCCTGACCCGCCGCTCATCGACTCACTGCCTCTCCAAACTCGCCGGGTCGATTGAGTCCGAAATTCAAGCGTGGATCGACCGCGAGAGCTTAGAAAGCTTGACGCGAGCTCTGGAAGAAGACCCAATTCGCGACGAGGATGATTTGATCAAGCTACTGACTCAGTTCGAGGACAGAGTCGCGCAGGGGTTCAACCGCGAGTTGCAGGATTTGATCTTGAAGTCCAAAGTTTTCACCTCGCTTGAGTCAGTTCTGTGCGAATCGACTCGGTCATCGAAGCGAGTCAGAGAACGCTCTGCTTTCGCCATTGCAGCTCTCATCAAGTTCAATAAAGATGTTTTCGTGGGTCAAGTTTTGATGGGTCGGCCGACGATTAGCTCTCTGATAAAAATGGCGTCACCGGACGCGATCAGAGTGCTCTGCGCGCTCGTCAGGCTGATCAAATCGCCGCTCGTCGATGAGATCGAGTTCAACGGAGAAATACCCAAGATCATAAGCTTCCTGAATTCAGAAGATTTGGAAATGCGAGTGATGGCGATGGTCTGCGTGCTCGAAATCGGGTACTTCGGGCGGAAGGAGGCCGTTGATGCGATGCTAGAGGAGGCGTTGATTGAGAAGCTTATGGAGTTGCAGAGGTCGGAGCACGGCGGCGATTTGATGAGGGAGAGCAGGGAGAAGGGGTTCTTGGAGAGCCACCCGTTTGCGAGCTGCGCGGCGAGGTTCGCGGTGCAGTTAGAGGTGGGGGAGGGGCTGAGGCAGCGGGAGAGGAGGGCGTTTAAGCAGCAGATATTGGTGAAAGTGAGGGAGGCCTCCGTTTCCGACGCGGAGGCCGCCACCATTTCTGCGGAGGTTTTGTGGGGGGCTTCGCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

384

Amino Acids

42.82

Weight (kDa)

5.5

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02430
malus_domestica MD02G1021100.v1.1 MD15G1164200.v1.1
prunus_persica Prupe.7G250400_v2.0.a1
pyrus_communis pycom02g01870 pycom05g21450 pycom15g14730
rosa_chinensis RchiOBHm_Chr2g0087391
rosa_laevigata RLG00000015862
rosa_multiflora Rmu_sc0006064.1_g000006
rosa_roxburghii Rroxscaffold_2G00153600
rosa_rugosa Rorug01G0474800
rosa_samantha Rh2AG028300 Rh2BG028200 Rh2CG028500 Rh2DG028300
rosa_wichuraiana Rw2G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 3 cut(s) 276, 294, 730
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 2 cut(s) 259, 644
AcsI RAATTY 2 cut(s) 342, 784
AcyI GRCGYC 1 cut(s) 674
AfaI GTAC 1 cut(s) 842
AfiI CCNNNNNNNGG 2 cut(s) 22, 181
AgsI TTSAA 5 cut(s) 347, 472, 499, 607, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 947, 979
AloI GAACNNNNNNTCC 2 cut(s) 944, 976
Alw21I GWGCWC 5 cut(s) 153, 391, 699, 831, 924
Alw26I GTCTC 1 cut(s) 149
AlwI GGATC 1 cut(s) 362
Ama87I CYCGRG 4 cut(s) 110, 146, 152, 180
AoxI GGCC 6 cut(s) 31, 259, 644, 858, 1086, 1107
ApeKI GCWGC 4 cut(s) 590, 987, 1036, 1060
ApoI RAATTY 2 cut(s) 342, 784
Asp700I GAANNNNTTC 1 cut(s) 959
AspLEI GCGC 4 cut(s) 463, 704, 706, 992
AspS9I GGNCC 3 cut(s) 32, 49, 184
AsuC2I CCSGG 3 cut(s) 181, 182, 325
AsuHPI GGTGA 3 cut(s) 505, 669, 1084
AvaI CYCGRG 4 cut(s) 110, 146, 152, 180
AvaII GGWCC 2 cut(s) 49, 184
BanII GRGCYC 2 cut(s) 153, 391
BauI CACGAG 2 cut(s) 36, 77
BbsI GAAGAC 2 cut(s) 33, 405
Bbv12I GWGCWC 5 cut(s) 153, 391, 699, 831, 924
BbvCI CCTCAGC 2 cut(s) 266, 1031
BbvI GCAGC 4 cut(s) 602, 974, 1048, 1072
BccI CCATC 4 cut(s) 24, 631, 805, 811
BceAI ACGGC 4 cut(s) 46, 845, 940, 1132
BcgI CGANNNNNNTGC 2 cut(s) 676, 710
BclI TGATCA 2 cut(s) 426, 717
BcnI CCSGG 3 cut(s) 181, 182, 325
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 3 cut(s) 53, 96, 875
BfmI CTRYAG 1 cut(s) 83
Bme1390I CCNGG 3 cut(s) 181, 182, 325
Bme18I GGWCC 2 cut(s) 49, 184
BmeT110I CYCGRG 4 cut(s) 110, 146, 152, 180
BmgT120I GGNCC 3 cut(s) 32, 49, 184
BmiI GGNNCC 2 cut(s) 185, 186
BmrFI CCNGG 3 cut(s) 181, 182, 325
BmsI GCATC 3 cut(s) 279, 856, 861
BpiI GAAGAC 2 cut(s) 33, 405
BplI GAGNNNNNCTC 2 cut(s) 104, 136
Bpu10I CCTNAGC 2 cut(s) 266, 1031
BpuEI CTTGAG 2 cut(s) 29, 542
BpuMI CCSGG 3 cut(s) 181, 182, 325
BsaHI GRCGYC 1 cut(s) 674
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 180
BsaWI WCCGGW 1 cut(s) 679
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 181
Bse1I ACTGG 1 cut(s) 250
Bse3DI GCAATG 1 cut(s) 585
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 4 cut(s) 122, 171, 270, 424
BseLI CCNNNNNNNGG 2 cut(s) 22, 181
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 3 cut(s) 280, 455, 1022
BseNI ACTGG 1 cut(s) 250
BsePI GCGCGC 1 cut(s) 702
BseRI GAGGAG 3 cut(s) 134, 893, 1060
BseX3I CGGCCG 1 cut(s) 644
BseXI GCAGC 4 cut(s) 602, 974, 1048, 1072
BsgI GTGCAG 1 cut(s) 1028
Bsh1285I CGRYCG 2 cut(s) 361, 647
BshFI GGCC 6 cut(s) 33, 261, 646, 860, 1088, 1109
BsiEI CGRYCG 2 cut(s) 361, 647
BsiHKAI GWGCWC 5 cut(s) 153, 391, 699, 831, 924
BsiHKCI CYCGRG 4 cut(s) 110, 146, 152, 180
BsiSI CCGG 3 cut(s) 181, 324, 680
BslFI GGGAC 3 cut(s) 84, 197, 226
BslI CCNNNNNNNGG 2 cut(s) 22, 181
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 3 cut(s) 84, 197, 226
BsnI GGCC 6 cut(s) 33, 261, 646, 860, 1088, 1109
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 4 cut(s) 110, 146, 152, 180
Bsp1286I GDGCHC 5 cut(s) 153, 391, 699, 831, 924
Bsp143I GATC 7 cut(s) 354, 426, 492, 687, 717, 741, 768
Bsp68I TCGCGA 1 cut(s) 411
BspANI GGCC 6 cut(s) 33, 261, 646, 860, 1088, 1109
BspCNI CTCAG 3 cut(s) 279, 454, 1023
BspLI GGNNCC 2 cut(s) 185, 186
BspPI GGATC 1 cut(s) 362
BspTNI GGTCTC 1 cut(s) 149
BsrBI CCGCTC 3 cut(s) 276, 294, 730
BsrDI GCAATG 1 cut(s) 585
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 180
BssHII GCGCGC 1 cut(s) 702
BssMI GATC 7 cut(s) 354, 426, 492, 687, 717, 741, 768
BssNI GRCGYC 1 cut(s) 674
BssSI CACGAG 2 cut(s) 36, 77
Bst2BI CACGAG 2 cut(s) 36, 77
BstACI GRCGYC 1 cut(s) 674
BstC8I GCNNGC 4 cut(s) 387, 704, 827, 985
BstDEI CTNAG 4 cut(s) 266, 370, 441, 1031
BstF5I GGATG 4 cut(s) 122, 171, 270, 424
BstHHI GCGC 4 cut(s) 463, 704, 706, 992
BstKTI GATC 7 cut(s) 357, 429, 495, 690, 720, 744, 771
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 7 cut(s) 354, 426, 492, 687, 717, 741, 768
BstMCI CGRYCG 2 cut(s) 361, 647
BstSCI CCNGG 3 cut(s) 179, 180, 323
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 4 cut(s) 602, 974, 1048, 1072
BstV2I GAAGAC 2 cut(s) 33, 405
BstZI CGGCCG 1 cut(s) 644
BsuRI GGCC 6 cut(s) 33, 261, 646, 860, 1088, 1109
BtgZI GCGATG 2 cut(s) 830, 884
BtsCI GGATG 4 cut(s) 122, 171, 270, 424
BtsI GCAGTG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 304
BtuMI TCGCGA 1 cut(s) 411
Cac8I GCNNGC 4 cut(s) 387, 704, 827, 985
CfoI GCGC 4 cut(s) 463, 704, 706, 992
Cfr13I GGNCC 3 cut(s) 32, 49, 184
Cfr9I CCCGGG 1 cut(s) 180
CseI GACGC 4 cut(s) 391, 663, 692, 1109
Csp6I GTAC 1 cut(s) 841
CviQI GTAC 1 cut(s) 841
DdeI CTNAG 4 cut(s) 266, 370, 441, 1031
DpnI GATC 7 cut(s) 356, 428, 494, 689, 719, 743, 770
DpnII GATC 7 cut(s) 354, 426, 492, 687, 717, 741, 768
EaeI YGGCCR 2 cut(s) 259, 644
EagI CGGCCG 1 cut(s) 644
EciI GGCGGA 4 cut(s) 118, 187, 236, 865
Ecl136II GAGCTC 2 cut(s) 151, 389
EclXI CGGCCG 1 cut(s) 644
Eco147I AGGCCT 1 cut(s) 1088
Eco24I GRGCYC 2 cut(s) 153, 391
Eco31I GGTCTC 1 cut(s) 149
Eco47I GGWCC 2 cut(s) 49, 184
Eco52I CGGCCG 1 cut(s) 644
Eco53kI GAGCTC 2 cut(s) 151, 389
Eco88I CYCGRG 4 cut(s) 110, 146, 152, 180
EcoICRI GAGCTC 2 cut(s) 151, 389
EcoO109I RGGNCCY 2 cut(s) 49, 184
EcoRI GAATTC 1 cut(s) 784
EcoT38I GRGCYC 2 cut(s) 153, 391
FaiI YATR 2 cut(s) 773, 902
FalI AAGNNNNNCTT 2 cut(s) 947, 979
FaqI GGGAC 3 cut(s) 84, 197, 226
FauI CCCGC 2 cut(s) 296, 1032
FbaI TGATCA 2 cut(s) 426, 717
FokI GGATG 4 cut(s) 109, 158, 257, 431
FriOI GRGCYC 2 cut(s) 153, 391
FspBI CTAG 3 cut(s) 53, 96, 875
GlaI GCGC 4 cut(s) 462, 703, 705, 991
HaeIII GGCC 6 cut(s) 33, 261, 646, 860, 1088, 1109
HapII CCGG 3 cut(s) 181, 324, 680
HgaI GACGC 4 cut(s) 391, 663, 692, 1109
HhaI GCGC 4 cut(s) 463, 704, 706, 992
Hin1I GRCGYC 1 cut(s) 674
Hin6I GCGC 4 cut(s) 461, 702, 704, 990
HinP1I GCGC 4 cut(s) 461, 702, 704, 990
HindIII AAGCTT 3 cut(s) 376, 775, 896
HpaII CCGG 3 cut(s) 181, 324, 680
HphI GGTGA 3 cut(s) 505, 669, 1084
Hpy188I TCNGA 8 cut(s) 166, 340, 566, 663, 692, 790, 919, 1099
Hpy188III TCNNGA 7 cut(s) 101, 154, 283, 392, 410, 496, 781
Hpy99I CGWCG 4 cut(s) 416, 652, 737, 1103
HpyAV CCTTC 3 cut(s) 289, 847, 949
HpyCH4V TGCA 4 cut(s) 484, 590, 910, 1009
HpyF3I CTNAG 4 cut(s) 266, 370, 441, 1031
Hsp92I GRCGYC 1 cut(s) 674
HspAI GCGC 4 cut(s) 461, 702, 704, 990
KflI GGGWCCC 1 cut(s) 184
Ksp22I TGATCA 2 cut(s) 426, 717
Kzo9I GATC 7 cut(s) 354, 426, 492, 687, 717, 741, 768
LmnI GCTCC 3 cut(s) 18, 281, 919
Lsp1109I GCAGC 4 cut(s) 602, 974, 1048, 1072
LweI GCATC 3 cut(s) 279, 856, 861
MaeI CTAG 3 cut(s) 53, 96, 875
MaeIII GTNAC 1 cut(s) 675
MalI GATC 7 cut(s) 356, 428, 494, 689, 719, 743, 770
MbiI CCGCTC 3 cut(s) 276, 294, 730
MboI GATC 7 cut(s) 354, 426, 492, 687, 717, 741, 768
MboII GAAGA 4 cut(s) 38, 407, 410, 803
MhlI GDGCHC 5 cut(s) 153, 391, 699, 831, 924
MluCI AATT 3 cut(s) 342, 405, 784
MmeI TCCRAC 3 cut(s) 189, 897, 1122
MroXI GAANNNNTTC 1 cut(s) 959
MseI TTAA 2 cut(s) 135, 1056
MspA1I CMGCKG 2 cut(s) 258, 1039
MspI CCGG 3 cut(s) 181, 324, 680
MspR9I CCNGG 3 cut(s) 181, 182, 325
NciI CCSGG 3 cut(s) 181, 182, 325
NdeII GATC 7 cut(s) 354, 426, 492, 687, 717, 741, 768
NlaIV GGNNCC 2 cut(s) 185, 186
NmuCI GTSAC 1 cut(s) 675
NruI TCGCGA 1 cut(s) 411
PaeR7I CTCGAG 2 cut(s) 146, 152
PauI GCGCGC 1 cut(s) 702
PceI AGGCCT 1 cut(s) 1088
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 741
PdmI GAANNNNTTC 1 cut(s) 959
PfeI GAWTC 2 cut(s) 104, 539
PflFI GACNNNGTC 3 cut(s) 160, 455, 547
PpuMI RGGWCCY 2 cut(s) 49, 184
Psp124BI GAGCTC 2 cut(s) 153, 391
Psp5II RGGWCCY 2 cut(s) 49, 184
PspN4I GGNNCC 2 cut(s) 185, 186
PspPI GGNCC 3 cut(s) 32, 49, 184
PspPPI RGGWCCY 2 cut(s) 49, 184
PspXI VCTCGAGB 1 cut(s) 146
PsyI GACNNNGTC 3 cut(s) 160, 455, 547
PteI GCGCGC 1 cut(s) 702
RruI TCGCGA 1 cut(s) 411
RsaI GTAC 1 cut(s) 842
RsaNI GTAC 1 cut(s) 841
SacI GAGCTC 2 cut(s) 153, 391
SaqAI TTAA 2 cut(s) 135, 1056
Sau3AI GATC 7 cut(s) 354, 426, 492, 687, 717, 741, 768
Sau96I GGNCC 3 cut(s) 32, 49, 184
ScrFI CCNGG 3 cut(s) 181, 182, 325
SduI GDGCHC 5 cut(s) 153, 391, 699, 831, 924
SfaNI GCATC 3 cut(s) 279, 856, 861
SfcI CTRYAG 1 cut(s) 83
Sfr274I CTCGAG 2 cut(s) 146, 152
SinI GGWCC 2 cut(s) 49, 184
SlaI CTCGAG 2 cut(s) 146, 152
SmaI CCCGGG 1 cut(s) 182
SmlI CTYRAG 4 cut(s) 44, 146, 152, 521
SmoI CTYRAG 4 cut(s) 44, 146, 152, 521
Sse9I AATT 3 cut(s) 342, 405, 784
SseBI AGGCCT 1 cut(s) 1088
SspMI CTAG 3 cut(s) 53, 96, 875
SstI GAGCTC 2 cut(s) 153, 391
StuI AGGCCT 1 cut(s) 1088
StyD4I CCNGG 3 cut(s) 179, 180, 323
TaqII GACCGA 2 cut(s) 173, 537
TasI AATT 3 cut(s) 342, 405, 784
TauI GCSGC 6 cut(s) 261, 294, 730, 930, 995, 1112
TfiI GAWTC 2 cut(s) 104, 539
Tru1I TTAA 2 cut(s) 135, 1056
Tru9I TTAA 2 cut(s) 135, 1056
TscAI CASTG 1 cut(s) 311
TseFI GTSAC 1 cut(s) 675
TseI GCWGC 4 cut(s) 590, 987, 1036, 1060
Tsp45I GTSAC 1 cut(s) 675
TspGWI ACGGA 2 cut(s) 768, 1081
TspMI CCCGGG 1 cut(s) 180
TspRI CASTG 1 cut(s) 311
Tth111I GACNNNGTC 3 cut(s) 160, 455, 547
VpaK11BI GGWCC 2 cut(s) 49, 184
XapI RAATTY 2 cut(s) 342, 784
XhoI CTCGAG 2 cut(s) 146, 152
XmaI CCCGGG 1 cut(s) 180
XmnI GAANNNNTTC 1 cut(s) 959
XspI CTAG 3 cut(s) 53, 96, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.