Rw2G002250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014628
Physical Location & Seq
Reverse (-)
1850931 .. 1852111
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002250.1

Sequence Viewer

Length: 1143 bp
ATGGACATGGAAGACCTACCAAGGCAAGACAGCCCACGAGTCCTGAAGGTCCTGGAAGCTCTAAAACAAGCCTCCCAGGAGCTCCAAACCCACCCGAGTCCCGACTCGGCCGAGTCCAACTCGTCCGCCATTAACGCCCTCCTCGAGCTCGAGACCGAGTCCGACTCGCTCCTCTCCCGAGACCCACGTCTCTCCACCCTCTGCCAACACCTCGCCACCCTCAAAACCCTCGTCGCTTCCCTCCGGAAACCCAACACCCACCACAGCATAATTCGACTCGCCGGTTCGATCGAGTCGGAAATCCAGGCCTGGATCGACCGCGAGAGCCTCGAGTCCCTAACGAGAGCTCTGAAACAAAAGGACCCGGTTGGCAACGAGGACGAGTTGATACAGCTACTGACTCGGTTCGAGGACCGAGTCTCGCAAGGCTTCAACCGCGAGTTGCAGGATTTGGTGTTGAAGTCCAAAGTGTTGAACTTGCTCGAATCTGTTCTCTGCGATTCGAATACCTCAAAGCGAGTCAGGGAACACTGCGCTTTCGCCATAGCTGCTTTGATCAAGTTCAACAAGGATGTGTTTGTGGGCCAAGTACTAATGGGTGCCACCATTAAAGCTTTGTTAACAACGGCTTCAACGAACTCGATCAAAGTGTTGTGTACTTTGGTCAGAACTATTAAGTCTCCGCTTGTGGATGAGATTGAGTTTAACGGTCAGATACCGAAGATTATAGGCCTGTTGAATTGGGACGACATTGAAATGAGAGTCATGGCGTTTGTTTGTGTGCTCGAAATTGGTTACTTCGGGCGCAAGGAAGCGATTGACGCAATGTTGGGGGAAGGTTTGATAGAGAAGCTTGTGGAGTTACAGAGGTCCGAGCTTGGTGGGGATTTGATGGAACTTGAAATGTATGATGAGGAAGGCGAAAACAAGGGCGGCTGCAGAGAGAAGAGGTTGTTGGAGAGGAGGCCGTTTTCGAGCTGTGTGGCGAGGTTTGCTGTGCAGTTGGAGGTGGGTGAGGGGCTGAGGCAGAGAGAGAGGAGGGCGTTCAAGCAGCAGATATTGGTGAGAGTTAGAGAAGCCTCCATTTCTGATGCAGAGGCTGCTACTATTATTGCTGAGGTTTTGTGGGGGTCTTCACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

380

Amino Acids

42.54

Weight (kDa)

5.23

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02430
malus_domestica MD02G1021100.v1.1 MD15G1164200.v1.1
prunus_persica Prupe.7G250400_v2.0.a1
pyrus_communis pycom02g01870 pycom05g21450 pycom15g14730
rosa_chinensis RchiOBHm_Chr2g0087391
rosa_laevigata RLG00000015862
rosa_multiflora Rmu_sc0006064.1_g000006
rosa_roxburghii Rroxscaffold_2G00153600
rosa_rugosa Rorug01G0474800
rosa_samantha Rh2AG028300 Rh2BG028200 Rh2CG028500 Rh2DG028300
rosa_wichuraiana Rw2G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 599
AccII CGCG 2 cut(s) 321, 438
AccIII TCCGGA 1 cut(s) 243
AciI CCGC 5 cut(s) 126, 319, 436, 683, 933
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 1 cut(s) 108
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 2 cut(s) 591, 658
AgsI TTSAA 9 cut(s) 433, 460, 475, 565, 633, 739, 755, 902, 1048
AjiI CACGTC 1 cut(s) 188
AjnI CCWGG 4 cut(s) 51, 75, 303, 308
AjuI GAANNNNNNNTTGG 2 cut(s) 938, 970
Alw21I GWGCWC 4 cut(s) 84, 150, 349, 786
Alw26I GTCTC 5 cut(s) 146, 174, 194, 424, 684
AlwI GGATC 1 cut(s) 320
AlwNI CAGNNNCTG 2 cut(s) 397, 1100
Ama87I CYCGRG 5 cut(s) 94, 143, 149, 177, 329
Aor13HI TCCGGA 1 cut(s) 243
AoxI GGCC 5 cut(s) 108, 306, 583, 730, 965
ApeKI GCWGC 4 cut(s) 548, 936, 1051, 1100
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
Asp700I GAANNNNTTC 1 cut(s) 489
AspLEI GCGC 2 cut(s) 536, 807
AspS9I GGNCC 5 cut(s) 49, 361, 412, 583, 870
AsuC2I CCSGG 1 cut(s) 365
AsuHPI GGTGA 3 cut(s) 1025, 1075, 1128
AsuII TTCGAA 1 cut(s) 503
AvaI CYCGRG 5 cut(s) 94, 143, 149, 177, 329
AvaII GGWCC 4 cut(s) 49, 361, 412, 870
BanI GGYRCC 1 cut(s) 599
BanII GRGCYC 3 cut(s) 84, 150, 349
BauI CACGAG 1 cut(s) 36
BbsI GAAGAC 2 cut(s) 18, 1125
Bbv12I GWGCWC 4 cut(s) 84, 150, 349, 786
BbvCI CCTCAGC 2 cut(s) 1022, 1116
BbvI GCAGC 4 cut(s) 535, 923, 1063, 1087
BccI CCATC 1 cut(s) 886
BceAI ACGGC 2 cut(s) 642, 952
BciT130I CCWGG 4 cut(s) 53, 77, 305, 310
BclI TGATCA 1 cut(s) 555
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 5 cut(s) 146, 174, 194, 424, 684
BfmI CTRYAG 1 cut(s) 937
BisI GCNGC 5 cut(s) 549, 934, 937, 1052, 1101
BlsI GCNGC 5 cut(s) 550, 935, 938, 1053, 1102
BmcAI AGTACT 1 cut(s) 591
Bme1390I CCNGG 5 cut(s) 53, 77, 305, 310, 365
Bme18I GGWCC 4 cut(s) 49, 361, 412, 870
BmeT110I CYCGRG 5 cut(s) 94, 143, 149, 177, 329
BmgBI CACGTC 1 cut(s) 188
BmgT120I GGNCC 5 cut(s) 49, 361, 412, 583, 870
BmiI GGNNCC 2 cut(s) 363, 601
BmrFI CCNGG 5 cut(s) 53, 77, 305, 310, 365
BmsI GCATC 1 cut(s) 1081
BoxI GACNNNNGTC 1 cut(s) 186
BpiI GAAGAC 2 cut(s) 18, 1125
BplI GAGNNNNNCTC 4 cut(s) 104, 136, 149, 181
Bpu10I CCTNAGC 2 cut(s) 1022, 1116
Bpu14I TTCGAA 1 cut(s) 503
BpuMI CCSGG 1 cut(s) 365
BsaI GGTCTC 2 cut(s) 146, 174
BsaJI CCNNGG 2 cut(s) 20, 75
BsaWI WCCGGW 1 cut(s) 243
Bse118I RCCGGY 1 cut(s) 281
Bse3DI GCAATG 1 cut(s) 831
BseAI TCCGGA 1 cut(s) 243
BseBI CCWGG 4 cut(s) 53, 77, 305, 310
BseDI CCNNGG 2 cut(s) 20, 75
BseGI GGATG 2 cut(s) 577, 697
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 2 cut(s) 1013, 1107
BseRI GAGGAG 4 cut(s) 131, 161, 976, 1051
BseX3I CGGCCG 1 cut(s) 108
BseXI GCAGC 4 cut(s) 535, 923, 1063, 1087
BsgI GTGCAG 1 cut(s) 1019
Bsh1236I CGCG 2 cut(s) 321, 438
Bsh1285I CGRYCG 3 cut(s) 111, 291, 319
BshFI GGCC 5 cut(s) 110, 308, 585, 732, 967
BshNI GGYRCC 1 cut(s) 599
BsiEI CGRYCG 3 cut(s) 111, 291, 319
BsiHKAI GWGCWC 4 cut(s) 84, 150, 349, 786
BsiHKCI CYCGRG 5 cut(s) 94, 143, 149, 177, 329
BsiSI CCGG 3 cut(s) 244, 282, 365
BslFI GGGAC 3 cut(s) 84, 319, 758
BsmAI GTCTC 5 cut(s) 146, 174, 194, 424, 684
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 3 cut(s) 84, 319, 758
BsnI GGCC 5 cut(s) 110, 308, 585, 732, 967
Bso31I GGTCTC 2 cut(s) 146, 174
BsoBI CYCGRG 5 cut(s) 94, 143, 149, 177, 329
Bsp119I TTCGAA 1 cut(s) 503
Bsp1286I GDGCHC 4 cut(s) 84, 150, 349, 786
Bsp13I TCCGGA 1 cut(s) 243
Bsp143I GATC 4 cut(s) 288, 312, 555, 642
BspACI CCGC 5 cut(s) 126, 319, 436, 683, 933
BspANI GGCC 5 cut(s) 110, 308, 585, 732, 967
BspCNI CTCAG 2 cut(s) 1014, 1108
BspEI TCCGGA 1 cut(s) 243
BspFNI CGCG 2 cut(s) 321, 438
BspLI GGNNCC 2 cut(s) 363, 601
BspMAI CTGCAG 1 cut(s) 941
BspPI GGATC 1 cut(s) 320
BspT104I TTCGAA 1 cut(s) 503
BspT107I GGYRCC 1 cut(s) 599
BspTNI GGTCTC 2 cut(s) 146, 174
BsrDI GCAATG 1 cut(s) 831
BsrFI RCCGGY 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 2 cut(s) 20, 75
BssMI GATC 4 cut(s) 288, 312, 555, 642
BssSI CACGAG 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 20
Bst2BI CACGAG 1 cut(s) 36
Bst2UI CCWGG 4 cut(s) 53, 77, 305, 310
Bst4CI ACNGT 1 cut(s) 710
Bst6I CTCTTC 1 cut(s) 941
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstBI TTCGAA 1 cut(s) 503
BstDEI CTNAG 3 cut(s) 1022, 1116, 1140
BstF5I GGATG 2 cut(s) 577, 697
BstFNI CGCG 2 cut(s) 321, 438
BstHHI GCGC 2 cut(s) 536, 807
BstKTI GATC 4 cut(s) 291, 315, 558, 645
BstMAI GTCTC 5 cut(s) 146, 174, 194, 424, 684
BstMBI GATC 4 cut(s) 288, 312, 555, 642
BstMCI CGRYCG 3 cut(s) 111, 291, 319
BstMWI GCNNNNNNNGC 6 cut(s) 134, 435, 548, 821, 992, 1100
BstNI CCWGG 4 cut(s) 53, 77, 305, 310
BstPAI GACNNNNGTC 1 cut(s) 186
BstSCI CCNGG 5 cut(s) 51, 75, 303, 308, 363
BstSFI CTRYAG 1 cut(s) 937
BstUI CGCG 2 cut(s) 321, 438
BstV1I GCAGC 4 cut(s) 535, 923, 1063, 1087
BstV2I GAAGAC 2 cut(s) 18, 1125
BstZI CGGCCG 1 cut(s) 108
BsuRI GGCC 5 cut(s) 110, 308, 585, 732, 967
BtrI CACGTC 1 cut(s) 188
BtsCI GGATG 2 cut(s) 577, 697
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 1 cut(s) 529
CaiI CAGNNNCTG 2 cut(s) 397, 1100
CfoI GCGC 2 cut(s) 536, 807
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 5 cut(s) 49, 361, 412, 583, 870
CseI GACGC 1 cut(s) 830
Csp6I GTAC 2 cut(s) 590, 657
CviAII CATG 2 cut(s) 7, 766
CviQI GTAC 2 cut(s) 590, 657
DdeI CTNAG 3 cut(s) 1022, 1116, 1140
DpnI GATC 4 cut(s) 290, 314, 557, 644
DpnII GATC 4 cut(s) 288, 312, 555, 642
EaeI YGGCCR 1 cut(s) 108
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 941
EarI CTCTTC 1 cut(s) 941
EciI GGCGGA 1 cut(s) 115
Ecl136II GAGCTC 3 cut(s) 82, 148, 347
EclXI CGGCCG 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 20
Eco147I AGGCCT 2 cut(s) 308, 732
Eco24I GRGCYC 3 cut(s) 84, 150, 349
Eco31I GGTCTC 2 cut(s) 146, 174
Eco47I GGWCC 4 cut(s) 49, 361, 412, 870
Eco52I CGGCCG 1 cut(s) 108
Eco53kI GAGCTC 3 cut(s) 82, 148, 347
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 5 cut(s) 94, 143, 149, 177, 329
EcoICRI GAGCTC 3 cut(s) 82, 148, 347
EcoO109I RGGNCCY 2 cut(s) 49, 361
EcoRII CCWGG 4 cut(s) 51, 75, 303, 308
EcoT14I CCWWGG 1 cut(s) 20
EcoT38I GRGCYC 3 cut(s) 84, 150, 349
ErhI CCWWGG 1 cut(s) 20
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 2 cut(s) 10, 769
FaiI YATR 6 cut(s) 8, 269, 545, 728, 767, 909
FaqI GGGAC 3 cut(s) 84, 319, 758
FatI CATG 2 cut(s) 6, 765
FbaI TGATCA 1 cut(s) 555
Fnu4HI GCNGC 5 cut(s) 549, 934, 937, 1052, 1101
FokI GGATG 2 cut(s) 584, 704
FriOI GRGCYC 3 cut(s) 84, 150, 349
Fsp4HI GCNGC 5 cut(s) 549, 934, 937, 1052, 1101
GlaI GCGC 2 cut(s) 535, 806
GluI GCNGC 5 cut(s) 549, 934, 937, 1052, 1101
HaeIII GGCC 5 cut(s) 110, 308, 585, 732, 967
HapII CCGG 3 cut(s) 244, 282, 365
HgaI GACGC 1 cut(s) 830
HhaI GCGC 2 cut(s) 536, 807
Hin1II CATG 2 cut(s) 10, 769
Hin6I GCGC 2 cut(s) 534, 805
HinP1I GCGC 2 cut(s) 534, 805
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 2 cut(s) 612, 851
HpaI GTTAAC 1 cut(s) 621
HpaII CCGG 3 cut(s) 244, 282, 365
HphI GGTGA 3 cut(s) 1025, 1075, 1128
Hpy166II GTNNAC 2 cut(s) 621, 657
Hpy188I TCNGA 7 cut(s) 163, 298, 351, 668, 714, 874, 1090
Hpy188III TCNNGA 5 cut(s) 43, 101, 151, 177, 244
Hpy8I GTNNAC 2 cut(s) 621, 657
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 3 cut(s) 40, 830, 911
HpyCH4III ACNGT 1 cut(s) 710
HpyCH4IV ACGT 1 cut(s) 187
HpyCH4V TGCA 4 cut(s) 445, 939, 1000, 1094
HpyF10VI GCNNNNNNNGC 6 cut(s) 134, 435, 548, 821, 992, 1100
HpyF3I CTNAG 3 cut(s) 1022, 1116, 1140
HpySE526I ACGT 1 cut(s) 187
Hsp92II CATG 2 cut(s) 10, 769
HspAI GCGC 2 cut(s) 534, 805
Kpn2I TCCGGA 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 555
KspAI GTTAAC 1 cut(s) 621
Kzo9I GATC 4 cut(s) 288, 312, 555, 642
LmnI GCTCC 3 cut(s) 79, 87, 174
Lsp1109I GCAGC 4 cut(s) 535, 923, 1063, 1087
LweI GCATC 1 cut(s) 1081
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 2 cut(s) 794, 861
MalI GATC 4 cut(s) 290, 314, 557, 644
MboI GATC 4 cut(s) 288, 312, 555, 642
MboII GAAGA 4 cut(s) 23, 733, 958, 1125
MhlI GDGCHC 4 cut(s) 84, 150, 349, 786
MluCI AATT 3 cut(s) 270, 739, 789
MmeI TCCRAC 5 cut(s) 141, 186, 276, 936, 984
MroI TCCGGA 1 cut(s) 243
MroXI GAANNNNTTC 1 cut(s) 489
MseI TTAA 5 cut(s) 132, 609, 620, 675, 705
MslI CAYNNNNRTG 1 cut(s) 755
MspI CCGG 3 cut(s) 244, 282, 365
MspR9I CCNGG 5 cut(s) 53, 77, 305, 310, 365
MvaI CCWGG 4 cut(s) 53, 77, 305, 310
MvnI CGCG 2 cut(s) 321, 438
MwoI GCNNNNNNNGC 6 cut(s) 134, 435, 548, 821, 992, 1100
NciI CCSGG 1 cut(s) 365
NdeII GATC 4 cut(s) 288, 312, 555, 642
NlaIII CATG 2 cut(s) 10, 769
NlaIV GGNNCC 2 cut(s) 363, 601
NmeAIII GCCGAG 2 cut(s) 86, 136
NspV TTCGAA 1 cut(s) 503
PaeR7I CTCGAG 3 cut(s) 143, 149, 329
PceI AGGCCT 2 cut(s) 308, 732
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 632
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 485, 500
PflFI GACNNNGTC 2 cut(s) 157, 416
PfoI TCCNGGA 1 cut(s) 51
PkrI GCNGC 5 cut(s) 550, 935, 938, 1053, 1102
Ple19I CGATCG 1 cut(s) 291
PpuMI RGGWCCY 2 cut(s) 49, 361
PshAI GACNNNNGTC 1 cut(s) 186
Psp124BI GAGCTC 3 cut(s) 84, 150, 349
Psp5II RGGWCCY 2 cut(s) 49, 361
Psp6I CCWGG 4 cut(s) 51, 75, 303, 308
PspGI CCWGG 4 cut(s) 51, 75, 303, 308
PspN4I GGNNCC 2 cut(s) 363, 601
PspPI GGNCC 5 cut(s) 49, 361, 412, 583, 870
PspPPI RGGWCCY 2 cut(s) 49, 361
PspXI VCTCGAGB 2 cut(s) 143, 329
PstI CTGCAG 1 cut(s) 941
PstNI CAGNNNCTG 2 cut(s) 397, 1100
PsyI GACNNNGTC 2 cut(s) 157, 416
PvuI CGATCG 1 cut(s) 291
RsaI GTAC 2 cut(s) 591, 658
RsaNI GTAC 2 cut(s) 590, 657
RseI CAYNNNNRTG 1 cut(s) 755
SacI GAGCTC 3 cut(s) 84, 150, 349
SaqAI TTAA 5 cut(s) 132, 609, 620, 675, 705
SatI GCNGC 5 cut(s) 549, 934, 937, 1052, 1101
Sau3AI GATC 4 cut(s) 288, 312, 555, 642
Sau96I GGNCC 5 cut(s) 49, 361, 412, 583, 870
ScaI AGTACT 1 cut(s) 591
ScrFI CCNGG 5 cut(s) 53, 77, 305, 310, 365
SduI GDGCHC 4 cut(s) 84, 150, 349, 786
SfaNI GCATC 1 cut(s) 1081
SfcI CTRYAG 1 cut(s) 937
Sfr274I CTCGAG 3 cut(s) 143, 149, 329
SfuI TTCGAA 1 cut(s) 503
SinI GGWCC 4 cut(s) 49, 361, 412, 870
SlaI CTCGAG 3 cut(s) 143, 149, 329
SmiMI CAYNNNNRTG 1 cut(s) 755
SmlI CTYRAG 3 cut(s) 143, 149, 329
SmoI CTYRAG 3 cut(s) 143, 149, 329
Sse9I AATT 3 cut(s) 270, 739, 789
SseBI AGGCCT 2 cut(s) 308, 732
SsiI CCGC 5 cut(s) 126, 319, 436, 683, 933
SstI GAGCTC 3 cut(s) 84, 150, 349
StuI AGGCCT 2 cut(s) 308, 732
StyD4I CCNGG 5 cut(s) 51, 75, 303, 308, 363
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 1 cut(s) 710
TaiI ACGT 1 cut(s) 190
TaqII GACCGA 2 cut(s) 170, 429
TasI AATT 3 cut(s) 270, 739, 789
TatI WGTACW 2 cut(s) 589, 656
TauI GCSGC 1 cut(s) 936
TfiI GAWTC 2 cut(s) 485, 500
Tru1I TTAA 5 cut(s) 132, 609, 620, 675, 705
Tru9I TTAA 5 cut(s) 132, 609, 620, 675, 705
TscAI CASTG 1 cut(s) 536
TseI GCWGC 4 cut(s) 548, 936, 1051, 1100
TspRI CASTG 1 cut(s) 536
Tth111I GACNNNGTC 2 cut(s) 157, 416
VpaK11BI GGWCC 4 cut(s) 49, 361, 412, 870
XhoI CTCGAG 3 cut(s) 143, 149, 329
XmnI GAANNNNTTC 1 cut(s) 489
ZrmI AGTACT 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.