pycom15g14730

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014628
Physical Location & Seq
Reverse (-)
10091457 .. 10092599
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14730.1

Sequence Viewer

Length: 1143 bp
ATGGAGGTGAAGCTCCATCAGAAGGAAGACGGCCCACGAGTTCTCAAGGTCCTAGAAGCTCTAAAACAAGCCTCCCACGAGCTACAGACCCACCCGATTCCCCACTCAAACTCATCCTCCGCCATTAACGCCCTCCTCGAGCTCGAGACCGAGTCCGACAACATCCTCTCCCGGGACCCACAATTCTCCGCCCTCTCCCAACACCTCGCCACCCTCAAAACCGTCGTCGAGTCCCTCCGCCAGTCCAGCGGCCACCTCAGCATCCGCTCCTTGCTTACTCGCTGCTGCTCGACTCACTGCCTCTCCAAACTCGCTCGGTCTATCGAGTCCGAAATTCAAGCGTGGATCGACCGCGAGTGCTTACATAGCTTGACGCGAGCTCTGGAAGAAGACCCAATTCGCGACGAGGACGAGTTGATCGAACTACTGACTCAGTTCGAGGACCGAGTTGCGCAGGGGTTCAACCATGAGTTGCAAGATTTGATCTTGAAGTCCAAAGTTGTCACCTCGCTCGAGTCAGTTCTGTGCGAGTCGACTCGGTCATCGAAGCGAGTCAAAGAACACACTGCTTTCGCCATTGCAGCTCTAATCAAGTTCAATAAAGATGTTTTCGTGGGTCAAGTTTTGATCGGTCGGCCGACGATCAGCTCTCTGATAAAAATCGCGTCTCCGAACGCGATCAGAGTACTCTGCGCGCTCGTCAGGCTGATCAAATCGCCGCTCGTCGACGAGATCGAGTTCAACGGAGAAATACCCCAGATCATAAGCTTCCTGAATTCCGAAGATTTGGAAATGCGAGTGATGGCGATGGTCTGCGTGTTCGAAATCGGGTATTTCGGGCGGAAGGAGGCTATTGACGCGATGCTACAGGAGGGGTTGATTGAGAAGCTTGTGGAGTTGCAGAGGTCGGAACACGGCGGCGATTTGATGAGGGAGAGCAGGGAGAAGGGGTTCTTGGAGAGCCACCCGTTTTCGAGCTGCGTGGCGAGGTTCGCAGTGCAGTTAGAGGTGGGGGAGGGGCTGAGGCAGCGGGAGAGGAGGGCGTTTAAGCAGCAGATATTGGTGAGAGTAAGGGAGGCGTCTATTTCCGACGCGGAGGCCGCCACCATTTTTGCGGAGGTTTTGTGGGGAGCTTCGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

381

Amino Acids

42.66

Weight (kDa)

5.64

Isoelectric Point (pI)

50.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02430
malus_domestica MD02G1021100.v1.1 MD15G1164200.v1.1
prunus_persica Prupe.7G250400_v2.0.a1
pyrus_communis pycom02g01870 pycom05g21450 pycom15g14730
rosa_chinensis RchiOBHm_Chr2g0087391
rosa_laevigata RLG00000015862
rosa_multiflora Rmu_sc0006064.1_g000006
rosa_roxburghii Rroxscaffold_2G00153600
rosa_rugosa Rorug01G0474800
rosa_samantha Rh2AG028300 Rh2BG028200 Rh2CG028500 Rh2DG028300
rosa_wichuraiana Rw2G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 453
AccBSI CCGCTC 2 cut(s) 267, 721
AccI GTMKAC 2 cut(s) 533, 726
AccII CGCG 8 cut(s) 354, 376, 402, 665, 677, 695, 860, 1094
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 2 cut(s) 250, 635
AcsI RAATTY 2 cut(s) 333, 775
AcyI GRCGYC 1 cut(s) 1079
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 2 cut(s) 22, 172
AgsI TTSAA 5 cut(s) 338, 463, 490, 598, 742
AjuI GAANNNNNNNTTGG 2 cut(s) 938, 970
AloI GAACNNNNNNTCC 2 cut(s) 935, 967
Alw21I GWGCWC 2 cut(s) 144, 382
Alw26I GTCTC 2 cut(s) 140, 672
AlwI GGATC 1 cut(s) 353
Ama87I CYCGRG 4 cut(s) 137, 143, 171, 512
AoxI GGCC 4 cut(s) 31, 250, 635, 1098
ApeKI GCWGC 6 cut(s) 282, 285, 581, 978, 1027, 1051
ApoI RAATTY 2 cut(s) 333, 775
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
Asp700I GAANNNNTTC 1 cut(s) 950
AspLEI GCGC 3 cut(s) 454, 695, 697
AspS9I GGNCC 4 cut(s) 32, 49, 175, 442
AsuC2I CCSGG 2 cut(s) 172, 173
AsuHPI GGTGA 3 cut(s) 19, 496, 1075
AsuII TTCGAA 1 cut(s) 822
AvaI CYCGRG 4 cut(s) 137, 143, 171, 512
AvaII GGWCC 3 cut(s) 49, 175, 442
BanII GRGCYC 2 cut(s) 144, 382
BauI CACGAG 2 cut(s) 36, 77
BbsI GAAGAC 2 cut(s) 33, 396
Bbv12I GWGCWC 2 cut(s) 144, 382
BbvCI CCTCAGC 2 cut(s) 257, 1022
BbvI GCAGC 6 cut(s) 269, 272, 593, 965, 1039, 1063
BccI CCATC 3 cut(s) 24, 796, 802
BceAI ACGGC 2 cut(s) 46, 931
BclI TGATCA 1 cut(s) 708
BcnI CCSGG 2 cut(s) 172, 173
BcoDI GTCTC 2 cut(s) 140, 672
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 2 cut(s) 83, 866
BmcAI AGTACT 1 cut(s) 687
Bme1390I CCNGG 2 cut(s) 172, 173
Bme18I GGWCC 3 cut(s) 49, 175, 442
BmeT110I CYCGRG 4 cut(s) 137, 143, 171, 512
BmgT120I GGNCC 4 cut(s) 32, 49, 175, 442
BmiI GGNNCC 2 cut(s) 176, 177
BmrFI CCNGG 2 cut(s) 172, 173
BmsI GCATC 2 cut(s) 270, 852
BpiI GAAGAC 2 cut(s) 33, 396
Bpu10I CCTNAGC 2 cut(s) 257, 1022
Bpu14I TTCGAA 1 cut(s) 822
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 2 cut(s) 172, 173
BsaBI GATNNNNATC 1 cut(s) 659
BsaHI GRCGYC 1 cut(s) 1079
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 171
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 172
Bse1I ACTGG 1 cut(s) 241
Bse3DI GCAATG 1 cut(s) 576
Bse8I GATNNNNATC 1 cut(s) 659
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 3 cut(s) 113, 162, 261
BseJI GATNNNNATC 1 cut(s) 659
BseLI CCNNNNNNNGG 2 cut(s) 22, 172
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 3 cut(s) 271, 446, 1013
BseNI ACTGG 1 cut(s) 241
BsePI GCGCGC 1 cut(s) 693
BseRI GAGGAG 2 cut(s) 125, 1051
BseX3I CGGCCG 1 cut(s) 635
BseXI GCAGC 6 cut(s) 269, 272, 593, 965, 1039, 1063
BsgI GTGCAG 1 cut(s) 1019
Bsh1236I CGCG 8 cut(s) 354, 376, 402, 665, 677, 695, 860, 1094
Bsh1285I CGRYCG 3 cut(s) 352, 634, 638
BshFI GGCC 4 cut(s) 33, 252, 637, 1100
BsiEI CGRYCG 3 cut(s) 352, 634, 638
BsiHKAI GWGCWC 2 cut(s) 144, 382
BsiHKCI CYCGRG 4 cut(s) 137, 143, 171, 512
BsiSI CCGG 1 cut(s) 172
BslFI GGGAC 2 cut(s) 188, 217
BslI CCNNNNNNNGG 2 cut(s) 22, 172
BsmAI GTCTC 2 cut(s) 140, 672
BsmBI CGTCTC 1 cut(s) 672
BsmFI GGGAC 2 cut(s) 188, 217
BsnI GGCC 4 cut(s) 33, 252, 637, 1100
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 4 cut(s) 137, 143, 171, 512
Bsp119I TTCGAA 1 cut(s) 822
Bsp1286I GDGCHC 2 cut(s) 144, 382
Bsp143I GATC 9 cut(s) 345, 417, 483, 627, 642, 678, 708, 732, 759
Bsp68I TCGCGA 1 cut(s) 402
BspANI GGCC 4 cut(s) 33, 252, 637, 1100
BspCNI CTCAG 3 cut(s) 270, 445, 1014
BspFNI CGCG 8 cut(s) 354, 376, 402, 665, 677, 695, 860, 1094
BspLI GGNNCC 2 cut(s) 176, 177
BspPI GGATC 1 cut(s) 353
BspT104I TTCGAA 1 cut(s) 822
BspTNI GGTCTC 1 cut(s) 140
BsrBI CCGCTC 2 cut(s) 267, 721
BsrDI GCAATG 1 cut(s) 576
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 171
BssHII GCGCGC 1 cut(s) 693
BssMI GATC 9 cut(s) 345, 417, 483, 627, 642, 678, 708, 732, 759
BssNI GRCGYC 1 cut(s) 1079
BssSI CACGAG 2 cut(s) 36, 77
Bst2BI CACGAG 2 cut(s) 36, 77
Bst4CI ACNGT 1 cut(s) 223
BstACI GRCGYC 1 cut(s) 1079
BstBI TTCGAA 1 cut(s) 822
BstC8I GCNNGC 2 cut(s) 378, 695
BstDEI CTNAG 3 cut(s) 257, 432, 1022
BstF5I GGATG 3 cut(s) 113, 162, 261
BstFNI CGCG 8 cut(s) 354, 376, 402, 665, 677, 695, 860, 1094
BstHHI GCGC 3 cut(s) 454, 695, 697
BstKTI GATC 9 cut(s) 348, 420, 486, 630, 645, 681, 711, 735, 762
BstMAI GTCTC 2 cut(s) 140, 672
BstMBI GATC 9 cut(s) 345, 417, 483, 627, 642, 678, 708, 732, 759
BstMCI CGRYCG 3 cut(s) 352, 634, 638
BstSCI CCNGG 2 cut(s) 170, 171
BstSFI CTRYAG 2 cut(s) 83, 866
BstUI CGCG 8 cut(s) 354, 376, 402, 665, 677, 695, 860, 1094
BstV1I GCAGC 6 cut(s) 269, 272, 593, 965, 1039, 1063
BstV2I GAAGAC 2 cut(s) 33, 396
BstZI CGGCCG 1 cut(s) 635
BsuRI GGCC 4 cut(s) 33, 252, 637, 1100
BtgZI GCGATG 2 cut(s) 821, 875
BtsCI GGATG 3 cut(s) 113, 162, 261
BtsI GCAGTG 3 cut(s) 295, 564, 1002
BtsIMutI CAGTG 3 cut(s) 295, 564, 1002
BtuMI TCGCGA 1 cut(s) 402
Cac8I GCNNGC 2 cut(s) 378, 695
CfoI GCGC 3 cut(s) 454, 695, 697
Cfr13I GGNCC 4 cut(s) 32, 49, 175, 442
Cfr9I CCCGGG 1 cut(s) 171
CseI GACGC 5 cut(s) 382, 654, 866, 1068, 1100
Csp6I GTAC 1 cut(s) 686
CspCI CAANNNNNGTGG 2 cut(s) 1093, 1128
CviAII CATG 1 cut(s) 467
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 3 cut(s) 257, 432, 1022
DpnI GATC 9 cut(s) 347, 419, 485, 629, 644, 680, 710, 734, 761
DpnII GATC 9 cut(s) 345, 417, 483, 627, 642, 678, 708, 732, 759
EaeI YGGCCR 2 cut(s) 250, 635
EagI CGGCCG 1 cut(s) 635
EciI GGCGGA 4 cut(s) 109, 178, 227, 856
Ecl136II GAGCTC 2 cut(s) 142, 380
EclXI CGGCCG 1 cut(s) 635
Eco24I GRGCYC 2 cut(s) 144, 382
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 3 cut(s) 49, 175, 442
Eco52I CGGCCG 1 cut(s) 635
Eco53kI GAGCTC 2 cut(s) 142, 380
Eco88I CYCGRG 4 cut(s) 137, 143, 171, 512
EcoICRI GAGCTC 2 cut(s) 142, 380
EcoO109I RGGNCCY 2 cut(s) 49, 175
EcoRI GAATTC 1 cut(s) 775
EcoT38I GRGCYC 2 cut(s) 144, 382
Esp3I CGTCTC 1 cut(s) 672
FaeI CATG 1 cut(s) 470
FaiI YATR 3 cut(s) 366, 468, 764
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FaqI GGGAC 2 cut(s) 188, 217
FatI CATG 1 cut(s) 466
FauI CCCGC 1 cut(s) 1023
FbaI TGATCA 1 cut(s) 708
FblI GTMKAC 2 cut(s) 533, 726
FokI GGATG 3 cut(s) 100, 149, 248
FriOI GRGCYC 2 cut(s) 144, 382
FspBI CTAG 1 cut(s) 53
FspI TGCGCA 1 cut(s) 453
GlaI GCGC 3 cut(s) 453, 694, 696
HaeIII GGCC 4 cut(s) 33, 252, 637, 1100
HapII CCGG 1 cut(s) 172
HgaI GACGC 5 cut(s) 382, 654, 866, 1068, 1100
HhaI GCGC 3 cut(s) 454, 695, 697
Hin1I GRCGYC 1 cut(s) 1079
Hin1II CATG 1 cut(s) 470
Hin6I GCGC 3 cut(s) 452, 693, 695
HinP1I GCGC 3 cut(s) 452, 693, 695
HincII GTYRAC 2 cut(s) 534, 727
HindII GTYRAC 2 cut(s) 534, 727
HindIII AAGCTT 2 cut(s) 766, 887
HpaII CCGG 1 cut(s) 172
HphI GGTGA 3 cut(s) 19, 496, 1075
Hpy166II GTNNAC 2 cut(s) 534, 727
Hpy188I TCNGA 9 cut(s) 21, 157, 331, 654, 672, 683, 781, 910, 1090
Hpy188III TCNNGA 5 cut(s) 145, 383, 401, 487, 772
Hpy8I GTNNAC 2 cut(s) 534, 727
Hpy99I CGWCG 7 cut(s) 227, 230, 407, 643, 728, 731, 1094
HpyAV CCTTC 3 cut(s) 16, 838, 940
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4V TGCA 4 cut(s) 475, 581, 901, 1000
HpyF3I CTNAG 3 cut(s) 257, 432, 1022
Hsp92I GRCGYC 1 cut(s) 1079
Hsp92II CATG 1 cut(s) 470
HspAI GCGC 3 cut(s) 452, 693, 695
KflI GGGWCCC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 708
Kzo9I GATC 9 cut(s) 345, 417, 483, 627, 642, 678, 708, 732, 759
LmnI GCTCC 3 cut(s) 18, 272, 1130
Lsp1109I GCAGC 6 cut(s) 269, 272, 593, 965, 1039, 1063
LweI GCATC 2 cut(s) 270, 852
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 1 cut(s) 502
MalI GATC 9 cut(s) 347, 419, 485, 629, 644, 680, 710, 734, 761
MbiI CCGCTC 2 cut(s) 267, 721
MboI GATC 9 cut(s) 345, 417, 483, 627, 642, 678, 708, 732, 759
MboII GAAGA 4 cut(s) 38, 398, 401, 794
MhlI GDGCHC 2 cut(s) 144, 382
MluCI AATT 4 cut(s) 182, 333, 396, 775
MlyI GAGTC 9 cut(s) 161, 239, 286, 335, 424, 524, 529, 539, 561
MmeI TCCRAC 3 cut(s) 180, 888, 1113
MroXI GAANNNNTTC 1 cut(s) 950
MseI TTAA 3 cut(s) 126, 1047, 1141
MspA1I CMGCKG 2 cut(s) 249, 1030
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 2 cut(s) 172, 173
MvnI CGCG 8 cut(s) 354, 376, 402, 665, 677, 695, 860, 1094
NciI CCSGG 2 cut(s) 172, 173
NdeII GATC 9 cut(s) 345, 417, 483, 627, 642, 678, 708, 732, 759
NlaIII CATG 1 cut(s) 470
NlaIV GGNNCC 2 cut(s) 176, 177
NmuCI GTSAC 1 cut(s) 502
NruI TCGCGA 1 cut(s) 402
NsbI TGCGCA 1 cut(s) 453
NspV TTCGAA 1 cut(s) 822
PaeR7I CTCGAG 3 cut(s) 137, 143, 512
PauI GCGCGC 1 cut(s) 693
PcsI WCGNNNNNNNCGW 3 cut(s) 135, 417, 732
PdmI GAANNNNTTC 1 cut(s) 950
PfeI GAWTC 1 cut(s) 97
PflFI GACNNNGTC 2 cut(s) 151, 538
PleI GAGTC 9 cut(s) 160, 238, 286, 334, 424, 523, 529, 538, 560
PpsI GAGTC 9 cut(s) 160, 238, 286, 334, 424, 523, 529, 538, 560
PpuMI RGGWCCY 2 cut(s) 49, 175
Psp124BI GAGCTC 2 cut(s) 144, 382
Psp5II RGGWCCY 2 cut(s) 49, 175
PspN4I GGNNCC 2 cut(s) 176, 177
PspPI GGNCC 4 cut(s) 32, 49, 175, 442
PspPPI RGGWCCY 2 cut(s) 49, 175
PspXI VCTCGAGB 2 cut(s) 137, 512
PsyI GACNNNGTC 2 cut(s) 151, 538
PteI GCGCGC 1 cut(s) 693
RruI TCGCGA 1 cut(s) 402
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
SacI GAGCTC 2 cut(s) 144, 382
SalI GTCGAC 2 cut(s) 532, 725
SaqAI TTAA 3 cut(s) 126, 1047, 1141
Sau3AI GATC 9 cut(s) 345, 417, 483, 627, 642, 678, 708, 732, 759
Sau96I GGNCC 4 cut(s) 32, 49, 175, 442
ScaI AGTACT 1 cut(s) 687
SchI GAGTC 9 cut(s) 161, 239, 286, 335, 424, 524, 529, 539, 561
ScrFI CCNGG 2 cut(s) 172, 173
SduI GDGCHC 2 cut(s) 144, 382
SfaNI GCATC 2 cut(s) 270, 852
SfcI CTRYAG 2 cut(s) 83, 866
Sfr274I CTCGAG 3 cut(s) 137, 143, 512
SfuI TTCGAA 1 cut(s) 822
SgrDI CGTCGACG 1 cut(s) 725
SinI GGWCC 3 cut(s) 49, 175, 442
SlaI CTCGAG 3 cut(s) 137, 143, 512
SmaI CCCGGG 1 cut(s) 173
SmlI CTYRAG 4 cut(s) 44, 137, 143, 512
SmoI CTYRAG 4 cut(s) 44, 137, 143, 512
Sse9I AATT 4 cut(s) 182, 333, 396, 775
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 2 cut(s) 144, 382
StyD4I CCNGG 2 cut(s) 170, 171
TaaI ACNGT 1 cut(s) 223
TaqII GACCGA 5 cut(s) 164, 306, 459, 528, 620
TasI AATT 4 cut(s) 182, 333, 396, 775
TatI WGTACW 1 cut(s) 685
TauI GCSGC 4 cut(s) 252, 721, 921, 1103
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 3 cut(s) 126, 1047, 1141
Tru9I TTAA 3 cut(s) 126, 1047, 1141
TscAI CASTG 3 cut(s) 302, 571, 1002
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 6 cut(s) 282, 285, 581, 978, 1027, 1051
Tsp45I GTSAC 1 cut(s) 502
TspGWI ACGGA 1 cut(s) 759
TspMI CCCGGG 1 cut(s) 171
TspRI CASTG 3 cut(s) 302, 571, 1002
Tth111I GACNNNGTC 2 cut(s) 151, 538
VpaK11BI GGWCC 3 cut(s) 49, 175, 442
XapI RAATTY 2 cut(s) 333, 775
XhoI CTCGAG 3 cut(s) 137, 143, 512
XmaI CCCGGG 1 cut(s) 171
XmiI GTMKAC 2 cut(s) 533, 726
XmnI GAANNNNTTC 1 cut(s) 950
XspI CTAG 1 cut(s) 53
ZrmI AGTACT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.