Rh2DG028300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014628
Physical Location & Seq
Reverse (-)
1712243 .. 1713424
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG028300.1

Sequence Viewer

Length: 1182 bp
ATGGACATGGAAGACCTACCAAGGCAAGACAGCCCACGAGTCCTGAAGGTCCTGGAAGCTCTAAAACAAGCCTCCCAGGAGCTCCAAACCCACCCGAGTCCCGACTCGGCCGAGTCCAACTCGTCCGCCATTAACGCCCTCCTCGAGCTCGAGACCGAGTCCGACTCGCTCCTCTCCCGAGACCCACATCTCTCCACTCTCTCCCAACACCTCGCCACCCTCAAAACCCTCGTCGCTTCCCTCCGGAAACCCACCGCCCACCACAGCATAATGTCCTTCCTCACTCGCCGCGCCTCCGCCCAGTCGCTGAGTCGACTCGCCGGTTCGATCGAGTCGGAAATCCAGGCCTGGATCGACCGCGAGAGCCTCGAGTCCCTAACGAGAGCTCTGAAACAGAAGGACCCAGTTGGCAACGAGGACGAGTTGGTCCAGCTACTGGCTCAGTTCGAGGACCGAGTCTCGCAAGGCTTCAACCGCGAGTTGCAGGATTTGGTGTTGAAGTCCAAAGTGTTGAACTTGCTCGAATCTGTTCTCTGCGATTCGAATACCTCAAAGCGAGTCAGGGAACACTGCGCTTTCGCCATAGCTGCTTTGATCAAGTTCAACAAGGATGTGTTTGTGGGCCAAGTACTAATGGGTGCCACCATTAAAGCTTTGTTAACAACGGCTTCAACGAACTCGATCAAAGTGTTGTGTACTTTGGTCAGAACTATAAAGTCTCCGCTTGTGGATGAGATTGAGTTTAACGGTCAGATACCGAAGATTATAGGCCTGTTGAATTGGGACGACATTGAAATGAGAGTCATGGCGTTTGTTTGTGTGCTCGAAATTGGTTACTTCGGGCGCAAGGAAGCGATTGACGCAATGTTGGGGGAAGGTTTGATAGAGAAGCTTGTGGAGTTACAGAGGTCCGAGCTTGGTGGGGATTTGATGGAACTTGAAATGTATGATGAGGAAGGCCAAAACAAGGGCGGCTGCAGAGAGAAGAGGTTGTTGGAGAGGCGGCCGTTTTCGAGCTGTGTGGCGAGGTTTGCTGTGCAGTTGGAGGTGGGTGAGGGGCTGAGGCAGAGAGAGAGGAGGGCGTTCAAGCAGCAGATATTGGTGAGAGTTAGAGAAGCCTCCATTTCTGATGCAGAGGCTGCTACTATTATTGCTGAGGTTTTGTGGGGGTCTTCACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

393

Amino Acids

43.81

Weight (kDa)

5.35

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02430
malus_domestica MD02G1021100.v1.1 MD15G1164200.v1.1
prunus_persica Prupe.7G250400_v2.0.a1
pyrus_communis pycom02g01870 pycom05g21450 pycom15g14730
rosa_chinensis RchiOBHm_Chr2g0087391
rosa_laevigata RLG00000015862
rosa_multiflora Rmu_sc0006064.1_g000006
rosa_roxburghii Rroxscaffold_2G00153600
rosa_rugosa Rorug01G0474800
rosa_samantha Rh2AG028300 Rh2BG028200 Rh2CG028500 Rh2DG028300
rosa_wichuraiana Rw2G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 638
AccB7I CCANNNNNTGG 1 cut(s) 436
AccI GTMKAC 1 cut(s) 313
AccII CGCG 3 cut(s) 291, 360, 477
AccIII TCCGGA 1 cut(s) 243
AciI CCGC 9 cut(s) 126, 255, 289, 297, 358, 475, 722, 972, 1003
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 2 cut(s) 108, 1004
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 2 cut(s) 630, 697
AfiI CCNNNNNNNGG 2 cut(s) 436, 967
AgsI TTSAA 9 cut(s) 472, 499, 514, 604, 672, 778, 794, 941, 1087
AjnI CCWGG 4 cut(s) 51, 75, 342, 347
AjuI GAANNNNNNNTTGG 2 cut(s) 977, 1009
Alw21I GWGCWC 4 cut(s) 84, 150, 388, 825
Alw26I GTCTC 4 cut(s) 146, 174, 463, 723
AlwI GGATC 1 cut(s) 359
AlwNI CAGNNNCTG 3 cut(s) 307, 436, 1139
Ama87I CYCGRG 5 cut(s) 94, 143, 149, 177, 368
Aor13HI TCCGGA 1 cut(s) 243
AoxI GGCC 6 cut(s) 108, 345, 622, 769, 958, 1004
ApeKI GCWGC 4 cut(s) 587, 975, 1090, 1139
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
Asp700I GAANNNNTTC 1 cut(s) 528
AspLEI GCGC 3 cut(s) 293, 575, 846
AspS9I GGNCC 6 cut(s) 49, 400, 427, 451, 622, 909
AsuHPI GGTGA 3 cut(s) 1064, 1114, 1167
AsuII TTCGAA 1 cut(s) 542
AvaI CYCGRG 5 cut(s) 94, 143, 149, 177, 368
AvaII GGWCC 5 cut(s) 49, 400, 427, 451, 909
BanI GGYRCC 1 cut(s) 638
BanII GRGCYC 3 cut(s) 84, 150, 388
BauI CACGAG 1 cut(s) 36
BbsI GAAGAC 2 cut(s) 18, 1164
Bbv12I GWGCWC 4 cut(s) 84, 150, 388, 825
BbvCI CCTCAGC 2 cut(s) 1061, 1155
BbvI GCAGC 4 cut(s) 574, 962, 1102, 1126
BccI CCATC 1 cut(s) 925
BceAI ACGGC 2 cut(s) 681, 991
BciT130I CCWGG 4 cut(s) 53, 77, 344, 349
BclI TGATCA 1 cut(s) 594
BcoDI GTCTC 4 cut(s) 146, 174, 463, 723
BfmI CTRYAG 1 cut(s) 976
BisI GCNGC 7 cut(s) 289, 588, 973, 976, 1004, 1091, 1140
BlsI GCNGC 7 cut(s) 290, 589, 974, 977, 1005, 1092, 1141
BmcAI AGTACT 1 cut(s) 630
Bme1390I CCNGG 4 cut(s) 53, 77, 344, 349
Bme18I GGWCC 5 cut(s) 49, 400, 427, 451, 909
BmeT110I CYCGRG 5 cut(s) 94, 143, 149, 177, 368
BmgT120I GGNCC 6 cut(s) 49, 400, 427, 451, 622, 909
BmiI GGNNCC 2 cut(s) 402, 640
BmrFI CCNGG 4 cut(s) 53, 77, 344, 349
BmrI ACTGGG 2 cut(s) 295, 398
BmsI GCATC 1 cut(s) 1120
BmuI ACTGGG 2 cut(s) 295, 398
BpiI GAAGAC 2 cut(s) 18, 1164
BplI GAGNNNNNCTC 4 cut(s) 104, 136, 149, 181
Bpu10I CCTNAGC 2 cut(s) 1061, 1155
Bpu14I TTCGAA 1 cut(s) 542
BsaI GGTCTC 2 cut(s) 146, 174
BsaJI CCNNGG 2 cut(s) 20, 75
BsaWI WCCGGW 1 cut(s) 243
Bsc4I CCNNNNNNNGG 2 cut(s) 436, 967
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 3 cut(s) 301, 404, 441
Bse3DI GCAATG 1 cut(s) 870
BseAI TCCGGA 1 cut(s) 243
BseBI CCWGG 4 cut(s) 53, 77, 344, 349
BseDI CCNNGG 2 cut(s) 20, 75
BseGI GGATG 2 cut(s) 616, 736
BseLI CCNNNNNNNGG 2 cut(s) 436, 967
BseMI GCAATG 1 cut(s) 870
BseMII CTCAG 4 cut(s) 299, 455, 1052, 1146
BseNI ACTGG 3 cut(s) 301, 404, 441
BseRI GAGGAG 3 cut(s) 131, 161, 1090
BseX3I CGGCCG 2 cut(s) 108, 1004
BseXI GCAGC 4 cut(s) 574, 962, 1102, 1126
BsgI GTGCAG 1 cut(s) 1058
Bsh1236I CGCG 3 cut(s) 291, 360, 477
Bsh1285I CGRYCG 4 cut(s) 111, 330, 358, 1007
BshFI GGCC 6 cut(s) 110, 347, 624, 771, 960, 1006
BshNI GGYRCC 1 cut(s) 638
BsiEI CGRYCG 4 cut(s) 111, 330, 358, 1007
BsiHKAI GWGCWC 4 cut(s) 84, 150, 388, 825
BsiHKCI CYCGRG 5 cut(s) 94, 143, 149, 177, 368
BsiSI CCGG 2 cut(s) 244, 321
BslFI GGGAC 3 cut(s) 84, 358, 797
BslI CCNNNNNNNGG 2 cut(s) 436, 967
BsmAI GTCTC 4 cut(s) 146, 174, 463, 723
BsmFI GGGAC 3 cut(s) 84, 358, 797
BsnI GGCC 6 cut(s) 110, 347, 624, 771, 960, 1006
Bso31I GGTCTC 2 cut(s) 146, 174
BsoBI CYCGRG 5 cut(s) 94, 143, 149, 177, 368
Bsp119I TTCGAA 1 cut(s) 542
Bsp1286I GDGCHC 4 cut(s) 84, 150, 388, 825
Bsp13I TCCGGA 1 cut(s) 243
Bsp143I GATC 4 cut(s) 327, 351, 594, 681
BspACI CCGC 9 cut(s) 126, 255, 289, 297, 358, 475, 722, 972, 1003
BspANI GGCC 6 cut(s) 110, 347, 624, 771, 960, 1006
BspCNI CTCAG 4 cut(s) 300, 454, 1053, 1147
BspEI TCCGGA 1 cut(s) 243
BspFNI CGCG 3 cut(s) 291, 360, 477
BspLI GGNNCC 2 cut(s) 402, 640
BspMAI CTGCAG 1 cut(s) 980
BspPI GGATC 1 cut(s) 359
BspT104I TTCGAA 1 cut(s) 542
BspT107I GGYRCC 1 cut(s) 638
BspTNI GGTCTC 2 cut(s) 146, 174
BsrDI GCAATG 1 cut(s) 870
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 3 cut(s) 301, 404, 441
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 2 cut(s) 20, 75
BssMI GATC 4 cut(s) 327, 351, 594, 681
BssSI CACGAG 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 20
Bst2BI CACGAG 1 cut(s) 36
Bst2UI CCWGG 4 cut(s) 53, 77, 344, 349
Bst4CI ACNGT 1 cut(s) 749
Bst6I CTCTTC 1 cut(s) 980
BstAPI GCANNNNNTGC 1 cut(s) 1139
BstBI TTCGAA 1 cut(s) 542
BstDEI CTNAG 5 cut(s) 308, 441, 1061, 1155, 1179
BstF5I GGATG 2 cut(s) 616, 736
BstFNI CGCG 3 cut(s) 291, 360, 477
BstHHI GCGC 3 cut(s) 293, 575, 846
BstKTI GATC 4 cut(s) 330, 354, 597, 684
BstMAI GTCTC 4 cut(s) 146, 174, 463, 723
BstMBI GATC 4 cut(s) 327, 351, 594, 681
BstMCI CGRYCG 4 cut(s) 111, 330, 358, 1007
BstMWI GCNNNNNNNGC 6 cut(s) 134, 474, 587, 860, 1031, 1139
BstNI CCWGG 4 cut(s) 53, 77, 344, 349
BstSCI CCNGG 4 cut(s) 51, 75, 342, 347
BstSFI CTRYAG 1 cut(s) 976
BstUI CGCG 3 cut(s) 291, 360, 477
BstV1I GCAGC 4 cut(s) 574, 962, 1102, 1126
BstV2I GAAGAC 2 cut(s) 18, 1164
BstZI CGGCCG 2 cut(s) 108, 1004
BsuRI GGCC 6 cut(s) 110, 347, 624, 771, 960, 1006
BtsCI GGATG 2 cut(s) 616, 736
BtsI GCAGTG 1 cut(s) 568
BtsIMutI CAGTG 1 cut(s) 568
CaiI CAGNNNCTG 3 cut(s) 307, 436, 1139
CfoI GCGC 3 cut(s) 293, 575, 846
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 6 cut(s) 49, 400, 427, 451, 622, 909
CseI GACGC 1 cut(s) 869
Csp6I GTAC 2 cut(s) 629, 696
CviAII CATG 2 cut(s) 7, 805
CviQI GTAC 2 cut(s) 629, 696
DdeI CTNAG 5 cut(s) 308, 441, 1061, 1155, 1179
DpnI GATC 4 cut(s) 329, 353, 596, 683
DpnII GATC 4 cut(s) 327, 351, 594, 681
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 2 cut(s) 108, 1004
EagI CGGCCG 2 cut(s) 108, 1004
Eam1104I CTCTTC 1 cut(s) 980
EarI CTCTTC 1 cut(s) 980
EciI GGCGGA 2 cut(s) 115, 286
Ecl136II GAGCTC 3 cut(s) 82, 148, 386
EclXI CGGCCG 2 cut(s) 108, 1004
Eco130I CCWWGG 1 cut(s) 20
Eco147I AGGCCT 2 cut(s) 347, 771
Eco24I GRGCYC 3 cut(s) 84, 150, 388
Eco31I GGTCTC 2 cut(s) 146, 174
Eco47I GGWCC 5 cut(s) 49, 400, 427, 451, 909
Eco52I CGGCCG 2 cut(s) 108, 1004
Eco53kI GAGCTC 3 cut(s) 82, 148, 386
Eco57I CTGAAG 1 cut(s) 65
Eco88I CYCGRG 5 cut(s) 94, 143, 149, 177, 368
EcoICRI GAGCTC 3 cut(s) 82, 148, 386
EcoO109I RGGNCCY 2 cut(s) 49, 400
EcoRII CCWGG 4 cut(s) 51, 75, 342, 347
EcoT14I CCWWGG 1 cut(s) 20
EcoT38I GRGCYC 3 cut(s) 84, 150, 388
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 2 cut(s) 10, 808
FaiI YATR 7 cut(s) 8, 269, 584, 713, 767, 806, 948
FaqI GGGAC 3 cut(s) 84, 358, 797
FatI CATG 2 cut(s) 6, 804
FbaI TGATCA 1 cut(s) 594
FblI GTMKAC 1 cut(s) 313
Fnu4HI GCNGC 7 cut(s) 289, 588, 973, 976, 1004, 1091, 1140
FokI GGATG 2 cut(s) 623, 743
FriOI GRGCYC 3 cut(s) 84, 150, 388
Fsp4HI GCNGC 7 cut(s) 289, 588, 973, 976, 1004, 1091, 1140
GlaI GCGC 3 cut(s) 292, 574, 845
GluI GCNGC 7 cut(s) 289, 588, 973, 976, 1004, 1091, 1140
HaeIII GGCC 6 cut(s) 110, 347, 624, 771, 960, 1006
HapII CCGG 2 cut(s) 244, 321
HgaI GACGC 1 cut(s) 869
HhaI GCGC 3 cut(s) 293, 575, 846
Hin1II CATG 2 cut(s) 10, 808
Hin6I GCGC 3 cut(s) 291, 573, 844
HinP1I GCGC 3 cut(s) 291, 573, 844
HincII GTYRAC 2 cut(s) 314, 660
HindII GTYRAC 2 cut(s) 314, 660
HindIII AAGCTT 2 cut(s) 651, 890
HpaI GTTAAC 1 cut(s) 660
HpaII CCGG 2 cut(s) 244, 321
HphI GGTGA 3 cut(s) 1064, 1114, 1167
Hpy166II GTNNAC 3 cut(s) 314, 660, 696
Hpy188I TCNGA 7 cut(s) 163, 337, 390, 707, 753, 913, 1129
Hpy188III TCNNGA 5 cut(s) 43, 101, 151, 177, 244
Hpy8I GTNNAC 3 cut(s) 314, 660, 696
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 5 cut(s) 40, 286, 391, 869, 950
HpyCH4III ACNGT 1 cut(s) 749
HpyCH4V TGCA 4 cut(s) 484, 978, 1039, 1133
HpyF10VI GCNNNNNNNGC 6 cut(s) 134, 474, 587, 860, 1031, 1139
HpyF3I CTNAG 5 cut(s) 308, 441, 1061, 1155, 1179
Hsp92II CATG 2 cut(s) 10, 808
HspAI GCGC 3 cut(s) 291, 573, 844
Kpn2I TCCGGA 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 594
KspAI GTTAAC 1 cut(s) 660
Kzo9I GATC 4 cut(s) 327, 351, 594, 681
LmnI GCTCC 3 cut(s) 79, 87, 174
Lsp1109I GCAGC 4 cut(s) 574, 962, 1102, 1126
LweI GCATC 1 cut(s) 1120
MaeIII GTNAC 2 cut(s) 833, 900
MalI GATC 4 cut(s) 329, 353, 596, 683
MboI GATC 4 cut(s) 327, 351, 594, 681
MboII GAAGA 4 cut(s) 23, 772, 997, 1164
MhlI GDGCHC 4 cut(s) 84, 150, 388, 825
MluCI AATT 2 cut(s) 778, 828
MmeI TCCRAC 5 cut(s) 141, 186, 315, 975, 1023
MroI TCCGGA 1 cut(s) 243
MroXI GAANNNNTTC 1 cut(s) 528
MseI TTAA 4 cut(s) 132, 648, 659, 744
MslI CAYNNNNRTG 1 cut(s) 794
MspI CCGG 2 cut(s) 244, 321
MspR9I CCNGG 4 cut(s) 53, 77, 344, 349
MvaI CCWGG 4 cut(s) 53, 77, 344, 349
MvnI CGCG 3 cut(s) 291, 360, 477
MwoI GCNNNNNNNGC 6 cut(s) 134, 474, 587, 860, 1031, 1139
NdeII GATC 4 cut(s) 327, 351, 594, 681
NlaIII CATG 2 cut(s) 10, 808
NlaIV GGNNCC 2 cut(s) 402, 640
NmeAIII GCCGAG 2 cut(s) 86, 136
NspV TTCGAA 1 cut(s) 542
PaeR7I CTCGAG 3 cut(s) 143, 149, 368
PceI AGGCCT 2 cut(s) 347, 771
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 671
PdmI GAANNNNTTC 1 cut(s) 528
PfeI GAWTC 2 cut(s) 524, 539
PflFI GACNNNGTC 2 cut(s) 157, 455
PflMI CCANNNNNTGG 1 cut(s) 436
PfoI TCCNGGA 1 cut(s) 51
PkrI GCNGC 7 cut(s) 290, 589, 974, 977, 1005, 1092, 1141
Ple19I CGATCG 1 cut(s) 330
PpuMI RGGWCCY 2 cut(s) 49, 400
Psp124BI GAGCTC 3 cut(s) 84, 150, 388
Psp5II RGGWCCY 2 cut(s) 49, 400
Psp6I CCWGG 4 cut(s) 51, 75, 342, 347
PspGI CCWGG 4 cut(s) 51, 75, 342, 347
PspN4I GGNNCC 2 cut(s) 402, 640
PspPI GGNCC 6 cut(s) 49, 400, 427, 451, 622, 909
PspPPI RGGWCCY 2 cut(s) 49, 400
PspXI VCTCGAGB 2 cut(s) 143, 368
PstI CTGCAG 1 cut(s) 980
PstNI CAGNNNCTG 3 cut(s) 307, 436, 1139
PsyI GACNNNGTC 2 cut(s) 157, 455
PvuI CGATCG 1 cut(s) 330
RsaI GTAC 2 cut(s) 630, 697
RsaNI GTAC 2 cut(s) 629, 696
RseI CAYNNNNRTG 1 cut(s) 794
SacI GAGCTC 3 cut(s) 84, 150, 388
SalI GTCGAC 1 cut(s) 312
SaqAI TTAA 4 cut(s) 132, 648, 659, 744
SatI GCNGC 7 cut(s) 289, 588, 973, 976, 1004, 1091, 1140
Sau3AI GATC 4 cut(s) 327, 351, 594, 681
Sau96I GGNCC 6 cut(s) 49, 400, 427, 451, 622, 909
ScaI AGTACT 1 cut(s) 630
ScrFI CCNGG 4 cut(s) 53, 77, 344, 349
SduI GDGCHC 4 cut(s) 84, 150, 388, 825
SfaNI GCATC 1 cut(s) 1120
SfcI CTRYAG 1 cut(s) 976
Sfr274I CTCGAG 3 cut(s) 143, 149, 368
SfuI TTCGAA 1 cut(s) 542
SinI GGWCC 5 cut(s) 49, 400, 427, 451, 909
SlaI CTCGAG 3 cut(s) 143, 149, 368
SmiMI CAYNNNNRTG 1 cut(s) 794
SmlI CTYRAG 3 cut(s) 143, 149, 368
SmoI CTYRAG 3 cut(s) 143, 149, 368
Sse9I AATT 2 cut(s) 778, 828
SseBI AGGCCT 2 cut(s) 347, 771
SsiI CCGC 9 cut(s) 126, 255, 289, 297, 358, 475, 722, 972, 1003
SstI GAGCTC 3 cut(s) 84, 150, 388
StuI AGGCCT 2 cut(s) 347, 771
StyD4I CCNGG 4 cut(s) 51, 75, 342, 347
StyI CCWWGG 1 cut(s) 20
TaaI ACNGT 1 cut(s) 749
TaqII GACCGA 2 cut(s) 170, 468
TasI AATT 2 cut(s) 778, 828
TatI WGTACW 2 cut(s) 628, 695
TauI GCSGC 3 cut(s) 291, 975, 1006
TfiI GAWTC 2 cut(s) 524, 539
Tru1I TTAA 4 cut(s) 132, 648, 659, 744
Tru9I TTAA 4 cut(s) 132, 648, 659, 744
TscAI CASTG 1 cut(s) 575
TseI GCWGC 4 cut(s) 587, 975, 1090, 1139
TspRI CASTG 1 cut(s) 575
Tth111I GACNNNGTC 2 cut(s) 157, 455
Van91I CCANNNNNTGG 1 cut(s) 436
VpaK11BI GGWCC 5 cut(s) 49, 400, 427, 451, 909
XhoI CTCGAG 3 cut(s) 143, 149, 368
XmiI GTMKAC 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 528
ZrmI AGTACT 1 cut(s) 630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.