RLG00000015862

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014628
Physical Location & Seq
Reverse (-)
1934458 .. 1935621
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015862

Sequence Viewer

Length: 1164 bp
ATGGAAGACCAACCAAGGCAAGACAGCCCGCGAGTCCTGAAGGTCCTGGAAGCTCTAAAACAAGCCTTCCAGCAGCTCCAAACCCACCCGAGTCCCGACTCGTCCGCCATTAACGCCCTCCTCGAGCTCGAGACCGAGTCCGACTCGCTCCTCTCCCGAGACCCACATCTCTCCACTCTCTCCCAACACCTCGCCACCCTCAAAACCCTCGTCGCTTCCCTCCGGAAATCCAACACCCACCACAGCATAATGGCCTTTCTGACTCGCCGCGCCTCCACTCAGTCCCTGAGTCGACTCGCCGGTTCGATCGAGTCGGAAATCCAGGCCTGGATCGACCGCGAGAGCCTCGAGTCCCTAACGAGAGCTCTGAAACAAAAGGACCCAGTTGACAACGAGGACGAGTTGATCAAGCTACTGACTCAGTTCGAGGACCGAGTCTCACAAGGCTTCAACCGCGAGTTTCAAGATTTGGTGTTGAAGTCCAAAGTGTTGAACTTGCTCGAATCTGTTCTCTGCGATTCGAATAGCTCAAAGCGAGTCAGGGAACACTGTGCTTTCGCCATAGCTGCTTTGATCAAGTTCAACAAGGATGTGTTTGTGGGTCAAGTACGAATGGGTGCCACCATCAAAGCTTTGTTAACAATGGCTTCACCTAACTCGATCAAAGTGTTATGTACTTTGGTCAGAACTATTAAGTCTCCGCTTGTAGATGAGATTGAGTTTAATGGTCAGATACCGAAGATTATAGGCCTGTTGAATTGGGACGACATTGAAATGAGAGTCCTGGCGTTTGTTTGTGTGCTCGAAATTGGTTACTTCGGGCGCAAGGAAGCGATTGACGCAATGTTGGAGGAAGGTTTGATAGAGAAGCTTGTGGAGTTACAGAGGTCCGAGCTTGGTGGGGATTTGATGGAACTTGAAATGTATGATGATGAGGAAGGCGAAAACAAGGGCGGCTGCAGAGAGAAGAGGTTGTTGGAGAGGCGGCCGTTTTCGAGCTGTGTGGCGAGGTTTGCTGTGCAGTTGGAGGTGGGTGAGGGGCTGAGGCAGAGAGAGAGGAGGGCGTTCAAGCAGCAGATATTGGTGAGAGTTAGAGAAGCCTCCATTTCTGATGCAGAGGCTGCTACTATTATTGCTGAGGTTTTGTGGGGGTCTTCACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

388

Amino Acids

43.56

Weight (kDa)

5.44

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02430
malus_domestica MD02G1021100.v1.1 MD15G1164200.v1.1
prunus_persica Prupe.7G250400_v2.0.a1
pyrus_communis pycom02g01870 pycom05g21450 pycom15g14730
rosa_chinensis RchiOBHm_Chr2g0087391
rosa_laevigata RLG00000015862
rosa_multiflora Rmu_sc0006064.1_g000006
rosa_roxburghii Rroxscaffold_2G00153600
rosa_rugosa Rorug01G0474800
rosa_samantha Rh2AG028300 Rh2BG028200 Rh2CG028500 Rh2DG028300
rosa_wichuraiana Rw2G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 617
AccI GTMKAC 1 cut(s) 292
AccII CGCG 4 cut(s) 31, 270, 339, 456
AccIII TCCGGA 1 cut(s) 222
AciI CCGC 8 cut(s) 29, 105, 268, 337, 454, 701, 954, 985
AclWI GGATC 1 cut(s) 338
AcoI YGGCCR 1 cut(s) 986
AcuI CTGAAG 1 cut(s) 59
AfaI GTAC 2 cut(s) 609, 676
AgsI TTSAA 9 cut(s) 451, 464, 478, 493, 583, 757, 773, 920, 1069
AjnI CCWGG 4 cut(s) 45, 321, 326, 783
AjuI GAANNNNNNNTTGG 2 cut(s) 959, 991
Alw21I GWGCWC 3 cut(s) 129, 367, 804
Alw26I GTCTC 4 cut(s) 125, 153, 442, 702
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 2 cut(s) 286, 1121
Ama87I CYCGRG 5 cut(s) 88, 122, 128, 156, 347
Aor13HI TCCGGA 1 cut(s) 222
AoxI GGCC 4 cut(s) 252, 324, 748, 986
ApeKI GCWGC 5 cut(s) 73, 566, 957, 1072, 1121
ArsI GACNNNNNNTTYG 2 cut(s) 195, 227
Asp700I GAANNNNTTC 1 cut(s) 507
AspLEI GCGC 2 cut(s) 272, 825
AspS9I GGNCC 4 cut(s) 43, 379, 430, 888
AsuHPI GGTGA 4 cut(s) 642, 1046, 1096, 1149
AsuII TTCGAA 1 cut(s) 521
AvaI CYCGRG 5 cut(s) 88, 122, 128, 156, 347
AvaII GGWCC 4 cut(s) 43, 379, 430, 888
BanI GGYRCC 1 cut(s) 617
BanII GRGCYC 2 cut(s) 129, 367
BbsI GAAGAC 2 cut(s) 12, 1146
Bbv12I GWGCWC 3 cut(s) 129, 367, 804
BbvCI CCTCAGC 2 cut(s) 1043, 1137
BbvI GCAGC 5 cut(s) 85, 553, 944, 1084, 1108
BccI CCATC 2 cut(s) 632, 904
BceAI ACGGC 1 cut(s) 973
BciT130I CCWGG 4 cut(s) 47, 323, 328, 785
BclI TGATCA 2 cut(s) 405, 573
BcoDI GTCTC 4 cut(s) 125, 153, 442, 702
BfmI CTRYAG 1 cut(s) 958
BisI GCNGC 8 cut(s) 74, 268, 567, 955, 958, 986, 1073, 1122
BlsI GCNGC 8 cut(s) 75, 269, 568, 956, 959, 987, 1074, 1123
Bme1390I CCNGG 4 cut(s) 47, 323, 328, 785
Bme18I GGWCC 4 cut(s) 43, 379, 430, 888
BmeT110I CYCGRG 5 cut(s) 88, 122, 128, 156, 347
BmgT120I GGNCC 4 cut(s) 43, 379, 430, 888
BmiI GGNNCC 2 cut(s) 381, 619
BmrFI CCNGG 4 cut(s) 47, 323, 328, 785
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 1 cut(s) 1102
BmuI ACTGGG 1 cut(s) 377
BpiI GAAGAC 2 cut(s) 12, 1146
BplI GAGNNNNNCTC 2 cut(s) 128, 160
Bpu10I CCTNAGC 2 cut(s) 1043, 1137
Bpu14I TTCGAA 1 cut(s) 521
BsaI GGTCTC 2 cut(s) 125, 153
BsaJI CCNNGG 1 cut(s) 14
BsaWI WCCGGW 1 cut(s) 222
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 1 cut(s) 383
Bse3DI GCAATG 1 cut(s) 849
BseAI TCCGGA 1 cut(s) 222
BseBI CCWGG 4 cut(s) 47, 323, 328, 785
BseDI CCNNGG 1 cut(s) 14
BseGI GGATG 1 cut(s) 595
BseMI GCAATG 1 cut(s) 849
BseMII CTCAG 5 cut(s) 278, 293, 434, 1034, 1128
BseNI ACTGG 1 cut(s) 383
BseRI GAGGAG 3 cut(s) 110, 140, 1072
BseX3I CGGCCG 1 cut(s) 986
BseXI GCAGC 5 cut(s) 85, 553, 944, 1084, 1108
BsgI GTGCAG 1 cut(s) 1040
Bsh1236I CGCG 4 cut(s) 31, 270, 339, 456
Bsh1285I CGRYCG 3 cut(s) 309, 337, 989
BshFI GGCC 4 cut(s) 254, 326, 750, 988
BshNI GGYRCC 1 cut(s) 617
BsiEI CGRYCG 3 cut(s) 309, 337, 989
BsiHKAI GWGCWC 3 cut(s) 129, 367, 804
BsiHKCI CYCGRG 5 cut(s) 88, 122, 128, 156, 347
BsiSI CCGG 2 cut(s) 223, 300
BslFI GGGAC 4 cut(s) 78, 268, 337, 776
BsmAI GTCTC 4 cut(s) 125, 153, 442, 702
BsmFI GGGAC 4 cut(s) 78, 268, 337, 776
BsnI GGCC 4 cut(s) 254, 326, 750, 988
Bso31I GGTCTC 2 cut(s) 125, 153
BsoBI CYCGRG 5 cut(s) 88, 122, 128, 156, 347
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 3 cut(s) 129, 367, 804
Bsp13I TCCGGA 1 cut(s) 222
Bsp143I GATC 5 cut(s) 306, 330, 405, 573, 660
BspACI CCGC 8 cut(s) 29, 105, 268, 337, 454, 701, 954, 985
BspANI GGCC 4 cut(s) 254, 326, 750, 988
BspCNI CTCAG 5 cut(s) 279, 292, 433, 1035, 1129
BspEI TCCGGA 1 cut(s) 222
BspFNI CGCG 4 cut(s) 31, 270, 339, 456
BspLI GGNNCC 2 cut(s) 381, 619
BspMAI CTGCAG 1 cut(s) 962
BspPI GGATC 1 cut(s) 338
BspT104I TTCGAA 1 cut(s) 521
BspT107I GGYRCC 1 cut(s) 617
BspTNI GGTCTC 2 cut(s) 125, 153
BsrDI GCAATG 1 cut(s) 849
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 1 cut(s) 383
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 14
BssMI GATC 5 cut(s) 306, 330, 405, 573, 660
BssT1I CCWWGG 1 cut(s) 14
Bst2UI CCWGG 4 cut(s) 47, 323, 328, 785
Bst4CI ACNGT 1 cut(s) 551
Bst6I CTCTTC 1 cut(s) 962
BstAPI GCANNNNNTGC 1 cut(s) 1121
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 29
BstDEI CTNAG 6 cut(s) 279, 287, 420, 1043, 1137, 1161
BstF5I GGATG 1 cut(s) 595
BstFNI CGCG 4 cut(s) 31, 270, 339, 456
BstHHI GCGC 2 cut(s) 272, 825
BstKTI GATC 5 cut(s) 309, 333, 408, 576, 663
BstMAI GTCTC 4 cut(s) 125, 153, 442, 702
BstMBI GATC 5 cut(s) 306, 330, 405, 573, 660
BstMCI CGRYCG 3 cut(s) 309, 337, 989
BstMWI GCNNNNNNNGC 6 cut(s) 113, 453, 566, 839, 1013, 1121
BstNI CCWGG 4 cut(s) 47, 323, 328, 785
BstSCI CCNGG 4 cut(s) 45, 321, 326, 783
BstSFI CTRYAG 1 cut(s) 958
BstUI CGCG 4 cut(s) 31, 270, 339, 456
BstV1I GCAGC 5 cut(s) 85, 553, 944, 1084, 1108
BstV2I GAAGAC 2 cut(s) 12, 1146
BstZI CGGCCG 1 cut(s) 986
BsuRI GGCC 4 cut(s) 254, 326, 750, 988
BtsCI GGATG 1 cut(s) 595
BtsIMutI CAGTG 1 cut(s) 547
Cac8I GCNNGC 1 cut(s) 29
CaiI CAGNNNCTG 2 cut(s) 286, 1121
CfoI GCGC 2 cut(s) 272, 825
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 4 cut(s) 43, 379, 430, 888
CseI GACGC 1 cut(s) 848
Csp6I GTAC 2 cut(s) 608, 675
CviQI GTAC 2 cut(s) 608, 675
DdeI CTNAG 6 cut(s) 279, 287, 420, 1043, 1137, 1161
DpnI GATC 5 cut(s) 308, 332, 407, 575, 662
DpnII GATC 5 cut(s) 306, 330, 405, 573, 660
EaeI YGGCCR 1 cut(s) 986
EagI CGGCCG 1 cut(s) 986
Eam1104I CTCTTC 1 cut(s) 962
EarI CTCTTC 1 cut(s) 962
EciI GGCGGA 1 cut(s) 94
Ecl136II GAGCTC 2 cut(s) 127, 365
EclXI CGGCCG 1 cut(s) 986
Eco130I CCWWGG 1 cut(s) 14
Eco147I AGGCCT 2 cut(s) 326, 750
Eco24I GRGCYC 2 cut(s) 129, 367
Eco31I GGTCTC 2 cut(s) 125, 153
Eco47I GGWCC 4 cut(s) 43, 379, 430, 888
Eco52I CGGCCG 1 cut(s) 986
Eco53kI GAGCTC 2 cut(s) 127, 365
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 5 cut(s) 88, 122, 128, 156, 347
EcoICRI GAGCTC 2 cut(s) 127, 365
EcoO109I RGGNCCY 2 cut(s) 43, 379
EcoRII CCWGG 4 cut(s) 45, 321, 326, 783
EcoT14I CCWWGG 1 cut(s) 14
EcoT38I GRGCYC 2 cut(s) 129, 367
ErhI CCWWGG 1 cut(s) 14
FaiI YATR 5 cut(s) 248, 563, 673, 746, 927
FaqI GGGAC 4 cut(s) 78, 268, 337, 776
FauI CCCGC 1 cut(s) 36
FbaI TGATCA 2 cut(s) 405, 573
FblI GTMKAC 1 cut(s) 292
Fnu4HI GCNGC 8 cut(s) 74, 268, 567, 955, 958, 986, 1073, 1122
FokI GGATG 1 cut(s) 602
FriOI GRGCYC 2 cut(s) 129, 367
Fsp4HI GCNGC 8 cut(s) 74, 268, 567, 955, 958, 986, 1073, 1122
GlaI GCGC 2 cut(s) 271, 824
GluI GCNGC 8 cut(s) 74, 268, 567, 955, 958, 986, 1073, 1122
HaeIII GGCC 4 cut(s) 254, 326, 750, 988
HapII CCGG 2 cut(s) 223, 300
HgaI GACGC 1 cut(s) 848
HhaI GCGC 2 cut(s) 272, 825
Hin6I GCGC 2 cut(s) 270, 823
HinP1I GCGC 2 cut(s) 270, 823
HincII GTYRAC 3 cut(s) 293, 388, 639
HindII GTYRAC 3 cut(s) 293, 388, 639
HindIII AAGCTT 2 cut(s) 630, 869
HpaI GTTAAC 1 cut(s) 639
HpaII CCGG 2 cut(s) 223, 300
HphI GGTGA 4 cut(s) 642, 1046, 1096, 1149
Hpy166II GTNNAC 3 cut(s) 293, 388, 639
Hpy188I TCNGA 8 cut(s) 142, 261, 316, 369, 686, 732, 892, 1111
Hpy188III TCNNGA 6 cut(s) 37, 95, 130, 156, 223, 464
Hpy8I GTNNAC 3 cut(s) 293, 388, 639
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 4 cut(s) 34, 76, 848, 932
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4V TGCA 3 cut(s) 960, 1021, 1115
HpyF10VI GCNNNNNNNGC 6 cut(s) 113, 453, 566, 839, 1013, 1121
HpyF3I CTNAG 6 cut(s) 279, 287, 420, 1043, 1137, 1161
HspAI GCGC 2 cut(s) 270, 823
Kpn2I TCCGGA 1 cut(s) 222
Ksp22I TGATCA 2 cut(s) 405, 573
KspAI GTTAAC 1 cut(s) 639
Kzo9I GATC 5 cut(s) 306, 330, 405, 573, 660
LmnI GCTCC 2 cut(s) 81, 153
Lsp1109I GCAGC 5 cut(s) 85, 553, 944, 1084, 1108
LweI GCATC 1 cut(s) 1102
MaeIII GTNAC 2 cut(s) 812, 879
MalI GATC 5 cut(s) 308, 332, 407, 575, 662
MboI GATC 5 cut(s) 306, 330, 405, 573, 660
MboII GAAGA 4 cut(s) 17, 751, 979, 1146
MhlI GDGCHC 3 cut(s) 129, 367, 804
MluCI AATT 2 cut(s) 757, 807
MmeI TCCRAC 6 cut(s) 165, 255, 294, 828, 957, 1005
MroI TCCGGA 1 cut(s) 222
MroXI GAANNNNTTC 1 cut(s) 507
MseI TTAA 4 cut(s) 111, 638, 693, 723
MslI CAYNNNNRTG 1 cut(s) 773
MspI CCGG 2 cut(s) 223, 300
MspR9I CCNGG 4 cut(s) 47, 323, 328, 785
MvaI CCWGG 4 cut(s) 47, 323, 328, 785
MvnI CGCG 4 cut(s) 31, 270, 339, 456
MwoI GCNNNNNNNGC 6 cut(s) 113, 453, 566, 839, 1013, 1121
NdeII GATC 5 cut(s) 306, 330, 405, 573, 660
NlaIV GGNNCC 2 cut(s) 381, 619
NspV TTCGAA 1 cut(s) 521
PaeR7I CTCGAG 3 cut(s) 122, 128, 347
PceI AGGCCT 2 cut(s) 326, 750
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 507
PfeI GAWTC 2 cut(s) 503, 518
PflFI GACNNNGTC 2 cut(s) 136, 434
PfoI TCCNGGA 1 cut(s) 45
PkrI GCNGC 8 cut(s) 75, 269, 568, 956, 959, 987, 1074, 1123
Ple19I CGATCG 1 cut(s) 309
PpuMI RGGWCCY 2 cut(s) 43, 379
Psp124BI GAGCTC 2 cut(s) 129, 367
Psp5II RGGWCCY 2 cut(s) 43, 379
Psp6I CCWGG 4 cut(s) 45, 321, 326, 783
PspGI CCWGG 4 cut(s) 45, 321, 326, 783
PspN4I GGNNCC 2 cut(s) 381, 619
PspPI GGNCC 4 cut(s) 43, 379, 430, 888
PspPPI RGGWCCY 2 cut(s) 43, 379
PspXI VCTCGAGB 2 cut(s) 122, 347
PstI CTGCAG 1 cut(s) 962
PstNI CAGNNNCTG 2 cut(s) 286, 1121
PsyI GACNNNGTC 2 cut(s) 136, 434
PvuI CGATCG 1 cut(s) 309
RsaI GTAC 2 cut(s) 609, 676
RsaNI GTAC 2 cut(s) 608, 675
RseI CAYNNNNRTG 1 cut(s) 773
SacI GAGCTC 2 cut(s) 129, 367
SalI GTCGAC 1 cut(s) 291
SaqAI TTAA 4 cut(s) 111, 638, 693, 723
SatI GCNGC 8 cut(s) 74, 268, 567, 955, 958, 986, 1073, 1122
Sau3AI GATC 5 cut(s) 306, 330, 405, 573, 660
Sau96I GGNCC 4 cut(s) 43, 379, 430, 888
ScrFI CCNGG 4 cut(s) 47, 323, 328, 785
SduI GDGCHC 3 cut(s) 129, 367, 804
SfaNI GCATC 1 cut(s) 1102
SfcI CTRYAG 1 cut(s) 958
Sfr274I CTCGAG 3 cut(s) 122, 128, 347
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 4 cut(s) 43, 379, 430, 888
SlaI CTCGAG 3 cut(s) 122, 128, 347
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 3 cut(s) 122, 128, 347
SmoI CTYRAG 3 cut(s) 122, 128, 347
Sse9I AATT 2 cut(s) 757, 807
SseBI AGGCCT 2 cut(s) 326, 750
SsiI CCGC 8 cut(s) 29, 105, 268, 337, 454, 701, 954, 985
SstI GAGCTC 2 cut(s) 129, 367
StuI AGGCCT 2 cut(s) 326, 750
StyD4I CCNGG 4 cut(s) 45, 321, 326, 783
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 1 cut(s) 551
TaqII GACCGA 2 cut(s) 149, 447
TasI AATT 2 cut(s) 757, 807
TatI WGTACW 1 cut(s) 674
TauI GCSGC 3 cut(s) 270, 957, 988
TfiI GAWTC 2 cut(s) 503, 518
Tru1I TTAA 4 cut(s) 111, 638, 693, 723
Tru9I TTAA 4 cut(s) 111, 638, 693, 723
TscAI CASTG 1 cut(s) 554
TseI GCWGC 5 cut(s) 73, 566, 957, 1072, 1121
TspRI CASTG 1 cut(s) 554
Tth111I GACNNNGTC 2 cut(s) 136, 434
VpaK11BI GGWCC 4 cut(s) 43, 379, 430, 888
XhoI CTCGAG 3 cut(s) 122, 128, 347
XmiI GTMKAC 1 cut(s) 292
XmnI GAANNNNTTC 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.