Rh2AG028300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014628
Physical Location & Seq
Reverse (-)
1963875 .. 1966557
2683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG028300.1

Sequence Viewer

Length: 1176 bp
ATGGAAGACCTACCAAGGCAAGACAGCCCACGAGTCCTGAAGGTCCTGGAAGCTCTAAAACAAGCCTCCCAGGAGCTCCAAACCCACCCGAGTCCCGACTCGGCCGAGTCCAACTCGTCCGCCATTAACGCCCTCCTCGAGCTCGAGACCGAGTCCGACTCGCTCCTCTCCCGAGACCCACGTCTCTCCACTCTCTCCCAACACCTCGCCACTCTCAAAACCCTCGTCGCTTCCCTCCGGAAACCCAACGCCCACCACAGCATAATGTCCTTCCTCACTCGCCGCGCCTCCGCCCAGTCGCTGAGTCGACTCGCCGGTTCGATCGAGTCGGAAATCCAGGCCTGGATCGACCGCGAGAGCCTCGAGTCCCTAACGAGAGCTCTGAAACAGAAGGACCCAGTTGGCAACGAGGACGAGTTGATCCAGCTACTGACTCGGTTCGAGGACCGAGTCTCGCAAGGCTTCAACCGCGAGTTGCAGGATTTGGTGTTGAAGTCCAAAGTGTTGAACTTGCTCGAATCTGTTCTCTGCGATTCGAATACCTCAAAGCGAGTCAGGGAACACTGCGCTTTCGCCATAGCTGCTTTGATCAAGTTCAACAAGGATGTGTTTGTGGGCCAAGTACTAATGGGTGCCACCATTAAAGCTTTGTTAACAACGGCTTCAACGAACTCGATCAAAGTGTTGTGTACTTTGGTCAGAACTATTAAGTCTCCGCTTGTGGATGAGATTGAGTTTAACGGTCAGATACCCAAGATTATAGGCCTGTTGAATTGGGACGACATTGAAATGAGAGTCATGGCGTTTGTCTGTGTGCTCGAAATTGGTTACTTCGGGCGCAAGGAAGCCATTGACGCAATGTTGGGGGAAGGTTTGATAGAGAAGCTTGTGGAGTTACAGAGGTCCGAGCTTGGTGGGGATTTGATGGAACTTGAAATGTATGATGAGGAAGGCCAAAACAAGGGCGGCTGCAGAGAGAAGAGGTTGTTGGAGAGGAGGCCGTTTTCGAGCTGTGTGGCGAGGTTTGCTGTGCAGTTGGAGGTGGGTGAGGGGCTGAGGCAGAGAGAGAGGAGGGCGTTCAAGCAGCAGATATTGGTGAGAGTTAGAGAAGCCTCCATTTCTGATGCAGAGGCTGCTACTATTATTGCTGAGGTTTTGTGGGGGTCTTCACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

391

Amino Acids

43.67

Weight (kDa)

5.55

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02430
malus_domestica MD02G1021100.v1.1 MD15G1164200.v1.1
prunus_persica Prupe.7G250400_v2.0.a1
pyrus_communis pycom02g01870 pycom05g21450 pycom15g14730
rosa_chinensis RchiOBHm_Chr2g0087391
rosa_laevigata RLG00000015862
rosa_multiflora Rmu_sc0006064.1_g000006
rosa_roxburghii Rroxscaffold_2G00153600
rosa_rugosa Rorug01G0474800
rosa_samantha Rh2AG028300 Rh2BG028200 Rh2CG028500 Rh2DG028300
rosa_wichuraiana Rw2G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 632
AccI GTMKAC 1 cut(s) 307
AccII CGCG 3 cut(s) 285, 354, 471
AccIII TCCGGA 1 cut(s) 237
AciI CCGC 7 cut(s) 120, 283, 291, 352, 469, 716, 966
AclWI GGATC 2 cut(s) 353, 415
AcoI YGGCCR 1 cut(s) 102
AcuI CTGAAG 1 cut(s) 59
AfaI GTAC 2 cut(s) 624, 691
AfiI CCNNNNNNNGG 1 cut(s) 961
AgsI TTSAA 9 cut(s) 466, 493, 508, 598, 666, 772, 788, 935, 1081
AjiI CACGTC 1 cut(s) 182
AjnI CCWGG 4 cut(s) 45, 69, 336, 341
AjuI GAANNNNNNNTTGG 2 cut(s) 971, 1003
Alw21I GWGCWC 4 cut(s) 78, 144, 382, 819
Alw26I GTCTC 5 cut(s) 140, 168, 188, 457, 717
AlwI GGATC 2 cut(s) 353, 415
AlwNI CAGNNNCTG 3 cut(s) 301, 430, 1133
Ama87I CYCGRG 5 cut(s) 88, 137, 143, 171, 362
Aor13HI TCCGGA 1 cut(s) 237
AoxI GGCC 6 cut(s) 102, 339, 616, 763, 952, 998
ApeKI GCWGC 4 cut(s) 581, 969, 1084, 1133
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
Asp700I GAANNNNTTC 1 cut(s) 522
AspLEI GCGC 3 cut(s) 287, 569, 840
AspS9I GGNCC 5 cut(s) 43, 394, 445, 616, 903
AsuHPI GGTGA 3 cut(s) 1058, 1108, 1161
AsuII TTCGAA 1 cut(s) 536
AvaI CYCGRG 5 cut(s) 88, 137, 143, 171, 362
AvaII GGWCC 4 cut(s) 43, 394, 445, 903
BanI GGYRCC 1 cut(s) 632
BanII GRGCYC 3 cut(s) 78, 144, 382
BauI CACGAG 1 cut(s) 30
BbsI GAAGAC 2 cut(s) 12, 1158
Bbv12I GWGCWC 4 cut(s) 78, 144, 382, 819
BbvCI CCTCAGC 2 cut(s) 1055, 1149
BbvI GCAGC 4 cut(s) 568, 956, 1096, 1120
BccI CCATC 1 cut(s) 919
BceAI ACGGC 2 cut(s) 675, 985
BciT130I CCWGG 4 cut(s) 47, 71, 338, 343
BclI TGATCA 1 cut(s) 588
BcoDI GTCTC 5 cut(s) 140, 168, 188, 457, 717
BfmI CTRYAG 1 cut(s) 970
BisI GCNGC 6 cut(s) 283, 582, 967, 970, 1085, 1134
BlsI GCNGC 6 cut(s) 284, 583, 968, 971, 1086, 1135
BmcAI AGTACT 1 cut(s) 624
Bme1390I CCNGG 4 cut(s) 47, 71, 338, 343
Bme18I GGWCC 4 cut(s) 43, 394, 445, 903
BmeT110I CYCGRG 5 cut(s) 88, 137, 143, 171, 362
BmgBI CACGTC 1 cut(s) 182
BmgT120I GGNCC 5 cut(s) 43, 394, 445, 616, 903
BmiI GGNNCC 2 cut(s) 396, 634
BmrFI CCNGG 4 cut(s) 47, 71, 338, 343
BmrI ACTGGG 2 cut(s) 289, 392
BmsI GCATC 1 cut(s) 1114
BmuI ACTGGG 2 cut(s) 289, 392
BoxI GACNNNNGTC 1 cut(s) 180
BpiI GAAGAC 2 cut(s) 12, 1158
BplI GAGNNNNNCTC 4 cut(s) 98, 130, 143, 175
Bpu10I CCTNAGC 2 cut(s) 1055, 1149
Bpu14I TTCGAA 1 cut(s) 536
BsaI GGTCTC 2 cut(s) 140, 168
BsaJI CCNNGG 2 cut(s) 14, 69
BsaWI WCCGGW 1 cut(s) 237
Bsc4I CCNNNNNNNGG 1 cut(s) 961
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 2 cut(s) 295, 398
Bse3DI GCAATG 1 cut(s) 864
BseAI TCCGGA 1 cut(s) 237
BseBI CCWGG 4 cut(s) 47, 71, 338, 343
BseDI CCNNGG 2 cut(s) 14, 69
BseGI GGATG 2 cut(s) 610, 730
BseLI CCNNNNNNNGG 1 cut(s) 961
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 3 cut(s) 293, 1046, 1140
BseNI ACTGG 2 cut(s) 295, 398
BseRI GAGGAG 4 cut(s) 125, 155, 1009, 1084
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 4 cut(s) 568, 956, 1096, 1120
BsgI GTGCAG 1 cut(s) 1052
Bsh1236I CGCG 3 cut(s) 285, 354, 471
Bsh1285I CGRYCG 3 cut(s) 105, 324, 352
BshFI GGCC 6 cut(s) 104, 341, 618, 765, 954, 1000
BshNI GGYRCC 1 cut(s) 632
BsiEI CGRYCG 3 cut(s) 105, 324, 352
BsiHKAI GWGCWC 4 cut(s) 78, 144, 382, 819
BsiHKCI CYCGRG 5 cut(s) 88, 137, 143, 171, 362
BsiSI CCGG 2 cut(s) 238, 315
BslFI GGGAC 3 cut(s) 78, 352, 791
BslI CCNNNNNNNGG 1 cut(s) 961
BsmAI GTCTC 5 cut(s) 140, 168, 188, 457, 717
BsmBI CGTCTC 1 cut(s) 188
BsmFI GGGAC 3 cut(s) 78, 352, 791
BsnI GGCC 6 cut(s) 104, 341, 618, 765, 954, 1000
Bso31I GGTCTC 2 cut(s) 140, 168
BsoBI CYCGRG 5 cut(s) 88, 137, 143, 171, 362
Bsp119I TTCGAA 1 cut(s) 536
Bsp1286I GDGCHC 4 cut(s) 78, 144, 382, 819
Bsp13I TCCGGA 1 cut(s) 237
Bsp143I GATC 5 cut(s) 321, 345, 420, 588, 675
BspACI CCGC 7 cut(s) 120, 283, 291, 352, 469, 716, 966
BspANI GGCC 6 cut(s) 104, 341, 618, 765, 954, 1000
BspCNI CTCAG 3 cut(s) 294, 1047, 1141
BspEI TCCGGA 1 cut(s) 237
BspFNI CGCG 3 cut(s) 285, 354, 471
BspLI GGNNCC 2 cut(s) 396, 634
BspMAI CTGCAG 1 cut(s) 974
BspPI GGATC 2 cut(s) 353, 415
BspT104I TTCGAA 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 632
BspTNI GGTCTC 2 cut(s) 140, 168
BsrDI GCAATG 1 cut(s) 864
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 2 cut(s) 295, 398
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 2 cut(s) 14, 69
BssMI GATC 5 cut(s) 321, 345, 420, 588, 675
BssSI CACGAG 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 14
Bst2BI CACGAG 1 cut(s) 30
Bst2UI CCWGG 4 cut(s) 47, 71, 338, 343
Bst4CI ACNGT 1 cut(s) 743
Bst6I CTCTTC 1 cut(s) 974
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstBI TTCGAA 1 cut(s) 536
BstDEI CTNAG 4 cut(s) 302, 1055, 1149, 1173
BstF5I GGATG 2 cut(s) 610, 730
BstFNI CGCG 3 cut(s) 285, 354, 471
BstHHI GCGC 3 cut(s) 287, 569, 840
BstKTI GATC 5 cut(s) 324, 348, 423, 591, 678
BstMAI GTCTC 5 cut(s) 140, 168, 188, 457, 717
BstMBI GATC 5 cut(s) 321, 345, 420, 588, 675
BstMCI CGRYCG 3 cut(s) 105, 324, 352
BstMWI GCNNNNNNNGC 6 cut(s) 128, 468, 581, 854, 1025, 1133
BstNI CCWGG 4 cut(s) 47, 71, 338, 343
BstPAI GACNNNNGTC 1 cut(s) 180
BstSCI CCNGG 4 cut(s) 45, 69, 336, 341
BstSFI CTRYAG 1 cut(s) 970
BstUI CGCG 3 cut(s) 285, 354, 471
BstV1I GCAGC 4 cut(s) 568, 956, 1096, 1120
BstV2I GAAGAC 2 cut(s) 12, 1158
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 6 cut(s) 104, 341, 618, 765, 954, 1000
BtrI CACGTC 1 cut(s) 182
BtsCI GGATG 2 cut(s) 610, 730
BtsI GCAGTG 1 cut(s) 562
BtsIMutI CAGTG 1 cut(s) 562
CaiI CAGNNNCTG 3 cut(s) 301, 430, 1133
CfoI GCGC 3 cut(s) 287, 569, 840
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 5 cut(s) 43, 394, 445, 616, 903
CseI GACGC 1 cut(s) 863
Csp6I GTAC 2 cut(s) 623, 690
CviAII CATG 1 cut(s) 799
CviQI GTAC 2 cut(s) 623, 690
DdeI CTNAG 4 cut(s) 302, 1055, 1149, 1173
DpnI GATC 5 cut(s) 323, 347, 422, 590, 677
DpnII GATC 5 cut(s) 321, 345, 420, 588, 675
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 974
EarI CTCTTC 1 cut(s) 974
EciI GGCGGA 2 cut(s) 109, 280
Ecl136II GAGCTC 3 cut(s) 76, 142, 380
EclXI CGGCCG 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 14
Eco147I AGGCCT 2 cut(s) 341, 765
Eco24I GRGCYC 3 cut(s) 78, 144, 382
Eco31I GGTCTC 2 cut(s) 140, 168
Eco47I GGWCC 4 cut(s) 43, 394, 445, 903
Eco52I CGGCCG 1 cut(s) 102
Eco53kI GAGCTC 3 cut(s) 76, 142, 380
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 5 cut(s) 88, 137, 143, 171, 362
EcoICRI GAGCTC 3 cut(s) 76, 142, 380
EcoO109I RGGNCCY 2 cut(s) 43, 394
EcoRII CCWGG 4 cut(s) 45, 69, 336, 341
EcoT14I CCWWGG 1 cut(s) 14
EcoT38I GRGCYC 3 cut(s) 78, 144, 382
ErhI CCWWGG 1 cut(s) 14
Esp3I CGTCTC 1 cut(s) 188
FaeI CATG 1 cut(s) 802
FaiI YATR 5 cut(s) 263, 578, 761, 800, 942
FaqI GGGAC 3 cut(s) 78, 352, 791
FatI CATG 1 cut(s) 798
FbaI TGATCA 1 cut(s) 588
FblI GTMKAC 1 cut(s) 307
Fnu4HI GCNGC 6 cut(s) 283, 582, 967, 970, 1085, 1134
FokI GGATG 2 cut(s) 617, 737
FriOI GRGCYC 3 cut(s) 78, 144, 382
Fsp4HI GCNGC 6 cut(s) 283, 582, 967, 970, 1085, 1134
GlaI GCGC 3 cut(s) 286, 568, 839
GluI GCNGC 6 cut(s) 283, 582, 967, 970, 1085, 1134
HaeIII GGCC 6 cut(s) 104, 341, 618, 765, 954, 1000
HapII CCGG 2 cut(s) 238, 315
HgaI GACGC 1 cut(s) 863
HhaI GCGC 3 cut(s) 287, 569, 840
Hin1II CATG 1 cut(s) 802
Hin6I GCGC 3 cut(s) 285, 567, 838
HinP1I GCGC 3 cut(s) 285, 567, 838
HincII GTYRAC 2 cut(s) 308, 654
HindII GTYRAC 2 cut(s) 308, 654
HindIII AAGCTT 2 cut(s) 645, 884
HpaI GTTAAC 1 cut(s) 654
HpaII CCGG 2 cut(s) 238, 315
HphI GGTGA 3 cut(s) 1058, 1108, 1161
Hpy166II GTNNAC 3 cut(s) 308, 654, 690
Hpy188I TCNGA 7 cut(s) 157, 331, 384, 701, 747, 907, 1123
Hpy188III TCNNGA 5 cut(s) 37, 95, 145, 171, 238
Hpy8I GTNNAC 3 cut(s) 308, 654, 690
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 5 cut(s) 34, 280, 385, 863, 944
HpyCH4III ACNGT 1 cut(s) 743
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 4 cut(s) 478, 972, 1033, 1127
HpyF10VI GCNNNNNNNGC 6 cut(s) 128, 468, 581, 854, 1025, 1133
HpyF3I CTNAG 4 cut(s) 302, 1055, 1149, 1173
HpySE526I ACGT 1 cut(s) 181
Hsp92II CATG 1 cut(s) 802
HspAI GCGC 3 cut(s) 285, 567, 838
Kpn2I TCCGGA 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 588
KspAI GTTAAC 1 cut(s) 654
Kzo9I GATC 5 cut(s) 321, 345, 420, 588, 675
LmnI GCTCC 3 cut(s) 73, 81, 168
Lsp1109I GCAGC 4 cut(s) 568, 956, 1096, 1120
LweI GCATC 1 cut(s) 1114
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 2 cut(s) 827, 894
MalI GATC 5 cut(s) 323, 347, 422, 590, 677
MboI GATC 5 cut(s) 321, 345, 420, 588, 675
MboII GAAGA 3 cut(s) 17, 991, 1158
MhlI GDGCHC 4 cut(s) 78, 144, 382, 819
MluCI AATT 2 cut(s) 772, 822
MmeI TCCRAC 5 cut(s) 135, 180, 309, 969, 1017
MroI TCCGGA 1 cut(s) 237
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 5 cut(s) 126, 642, 653, 708, 738
MslI CAYNNNNRTG 1 cut(s) 788
MspI CCGG 2 cut(s) 238, 315
MspR9I CCNGG 4 cut(s) 47, 71, 338, 343
MvaI CCWGG 4 cut(s) 47, 71, 338, 343
MvnI CGCG 3 cut(s) 285, 354, 471
MwoI GCNNNNNNNGC 6 cut(s) 128, 468, 581, 854, 1025, 1133
NdeII GATC 5 cut(s) 321, 345, 420, 588, 675
NlaIII CATG 1 cut(s) 802
NlaIV GGNNCC 2 cut(s) 396, 634
NmeAIII GCCGAG 2 cut(s) 80, 130
NspV TTCGAA 1 cut(s) 536
PaeR7I CTCGAG 3 cut(s) 137, 143, 362
PceI AGGCCT 2 cut(s) 341, 765
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 665
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 2 cut(s) 518, 533
PflFI GACNNNGTC 2 cut(s) 151, 449
PfoI TCCNGGA 1 cut(s) 45
PkrI GCNGC 6 cut(s) 284, 583, 968, 971, 1086, 1135
Ple19I CGATCG 1 cut(s) 324
PpuMI RGGWCCY 2 cut(s) 43, 394
PshAI GACNNNNGTC 1 cut(s) 180
Psp124BI GAGCTC 3 cut(s) 78, 144, 382
Psp5II RGGWCCY 2 cut(s) 43, 394
Psp6I CCWGG 4 cut(s) 45, 69, 336, 341
PspGI CCWGG 4 cut(s) 45, 69, 336, 341
PspN4I GGNNCC 2 cut(s) 396, 634
PspPI GGNCC 5 cut(s) 43, 394, 445, 616, 903
PspPPI RGGWCCY 2 cut(s) 43, 394
PspXI VCTCGAGB 2 cut(s) 137, 362
PstI CTGCAG 1 cut(s) 974
PstNI CAGNNNCTG 3 cut(s) 301, 430, 1133
PsyI GACNNNGTC 2 cut(s) 151, 449
PvuI CGATCG 1 cut(s) 324
RsaI GTAC 2 cut(s) 624, 691
RsaNI GTAC 2 cut(s) 623, 690
RseI CAYNNNNRTG 1 cut(s) 788
SacI GAGCTC 3 cut(s) 78, 144, 382
SalI GTCGAC 1 cut(s) 306
SaqAI TTAA 5 cut(s) 126, 642, 653, 708, 738
SatI GCNGC 6 cut(s) 283, 582, 967, 970, 1085, 1134
Sau3AI GATC 5 cut(s) 321, 345, 420, 588, 675
Sau96I GGNCC 5 cut(s) 43, 394, 445, 616, 903
ScaI AGTACT 1 cut(s) 624
ScrFI CCNGG 4 cut(s) 47, 71, 338, 343
SduI GDGCHC 4 cut(s) 78, 144, 382, 819
SfaNI GCATC 1 cut(s) 1114
SfcI CTRYAG 1 cut(s) 970
Sfr274I CTCGAG 3 cut(s) 137, 143, 362
SfuI TTCGAA 1 cut(s) 536
SinI GGWCC 4 cut(s) 43, 394, 445, 903
SlaI CTCGAG 3 cut(s) 137, 143, 362
SmiMI CAYNNNNRTG 1 cut(s) 788
SmlI CTYRAG 3 cut(s) 137, 143, 362
SmoI CTYRAG 3 cut(s) 137, 143, 362
Sse9I AATT 2 cut(s) 772, 822
SseBI AGGCCT 2 cut(s) 341, 765
SsiI CCGC 7 cut(s) 120, 283, 291, 352, 469, 716, 966
SstI GAGCTC 3 cut(s) 78, 144, 382
StuI AGGCCT 2 cut(s) 341, 765
StyD4I CCNGG 4 cut(s) 45, 69, 336, 341
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 1 cut(s) 743
TaiI ACGT 1 cut(s) 184
TaqII GACCGA 2 cut(s) 164, 462
TasI AATT 2 cut(s) 772, 822
TatI WGTACW 2 cut(s) 622, 689
TauI GCSGC 2 cut(s) 285, 969
TfiI GAWTC 2 cut(s) 518, 533
Tru1I TTAA 5 cut(s) 126, 642, 653, 708, 738
Tru9I TTAA 5 cut(s) 126, 642, 653, 708, 738
TscAI CASTG 1 cut(s) 569
TseI GCWGC 4 cut(s) 581, 969, 1084, 1133
TspRI CASTG 1 cut(s) 569
Tth111I GACNNNGTC 2 cut(s) 151, 449
VpaK11BI GGWCC 4 cut(s) 43, 394, 445, 903
XhoI CTCGAG 3 cut(s) 137, 143, 362
XmiI GTMKAC 1 cut(s) 307
XmnI GAANNNNTTC 1 cut(s) 522
ZrmI AGTACT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.