MD15G1164200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014628
Physical Location & Seq
Reverse (-)
12359882 .. 12361024
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1164200.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGAGGTCAAGCTCCATCAGAAGGAAGACGGCCCGCGAGTTCTCAAGGTCCTAGAAGCTCTAAAACAAGCCTCCCACGAGCTACAGACCCACCCGATTCCCCACTCAAACTCATCCTCCGCCATTAACGCCCTCCTCGAGCTCGAGACCGAGTCCGACAACATCCTCTCCCGGGACCCACAACTATCCGCCCTCTCCCAGCACCTCGCCACCCTCAAAACCCTCGTCGAGTCCCTCCGCCAGTCCAGCGGCCACCTCAGCATCCGCTCCTTCCTTACTCGGTGCTGCTCGACTCACTGCCTCTCCAAACTCGCCCGGTCTATCGAGTCCGAAATTCAAGCTTGGATCGACCGCGAGTGCTTACATAGCTTGACGCGAGCTCTGGAAGAAGATCCAATTCGCGACGAGGACAAGTTGATCAAACTACTGACTCGGTTCGAGGACCGAGTTGCGCAGGGGTTCAACCATGAGTTGCAAGATTTGATCTTGAAGTCCAAAATTGTCACCTCGCTCGAGTCTGTTCTGTGCGAGTCGACTTGGTCATCGAAGCGAGTCAAAGAACACATTGCTTTCGCCATTGCAGCTCTGATCAAGTTCAATAAAGATGTTTTCGTGGGTCAAGTTTTGATTGGTCGGCCGACGATCAGCTCTCTGATAAAAATCGCGTCTCCGAACGCGATCAGAGTACTCTGCGCGCTCGTCAGGCTGATCAAATCGCCGCTCGTCGACGAGATCGAGTTCAACGGAGAAATACCCAATATCATAAGCTTCCTGAATTCCGAAGATTTGGAAATGCGAGTGATGGCGATGGTCTGCGTGTTCGAAATCGGGTATTTCGGGCGGAAGGAGGCTATTGACGCGATGCTACAGGAGGGGTTGATTGAGAAGCTTGTGGAGTTGCAGAGGTCGGAGCACGGCGGTGATTTGATGAGGGAGAGCAGGGAGAAGGGGTTCTTGGAGAGCCACCCGTTTTCGAGCTGCGTGGCGAGGTTCACAGTGCAGTTAGAGGTGGGGGAGGGGCTGAGGCAGCGGGAGAGGAGGGCGTTTAAGCAGCAGATATTGGTGAGAGTAAGGGAGGCGTCTATTTCCGACGCGGAGGCTGCCACCATTTTTGCGGAGGTTTTGTGGGGAGCTTCGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

381

Amino Acids

42.77

Weight (kDa)

6.0

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02430
malus_domestica MD02G1021100.v1.1 MD15G1164200.v1.1
prunus_persica Prupe.7G250400_v2.0.a1
pyrus_communis pycom02g01870 pycom05g21450 pycom15g14730
rosa_chinensis RchiOBHm_Chr2g0087391
rosa_laevigata RLG00000015862
rosa_multiflora Rmu_sc0006064.1_g000006
rosa_roxburghii Rroxscaffold_2G00153600
rosa_rugosa Rorug01G0474800
rosa_samantha Rh2AG028300 Rh2BG028200 Rh2CG028500 Rh2DG028300
rosa_wichuraiana Rw2G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 453
AccBSI CCGCTC 2 cut(s) 267, 721
AccI GTMKAC 2 cut(s) 533, 726
AccII CGCG 9 cut(s) 37, 354, 376, 402, 665, 677, 695, 860, 1094
AclWI GGATC 2 cut(s) 353, 386
AcoI YGGCCR 2 cut(s) 250, 635
AcsI RAATTY 2 cut(s) 333, 775
AcyI GRCGYC 1 cut(s) 1079
AfaI GTAC 1 cut(s) 687
AfiI CCNNNNNNNGG 2 cut(s) 22, 172
AgsI TTSAA 5 cut(s) 338, 463, 490, 598, 742
AjuI GAANNNNNNNTTGG 2 cut(s) 938, 970
AleI CACNNNNGTG 1 cut(s) 918
AloI GAACNNNNNNTCC 2 cut(s) 935, 967
Alw21I GWGCWC 3 cut(s) 144, 382, 915
Alw26I GTCTC 2 cut(s) 140, 672
AlwI GGATC 2 cut(s) 353, 386
Ama87I CYCGRG 4 cut(s) 137, 143, 171, 512
AoxI GGCC 3 cut(s) 31, 250, 635
ApeKI GCWGC 6 cut(s) 285, 581, 978, 1027, 1051, 1100
ApoI RAATTY 2 cut(s) 333, 775
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
Asp700I GAANNNNTTC 1 cut(s) 950
AspLEI GCGC 3 cut(s) 454, 695, 697
AspS9I GGNCC 4 cut(s) 32, 49, 175, 442
AsuC2I CCSGG 3 cut(s) 172, 173, 316
AsuHPI GGTGA 3 cut(s) 496, 932, 1075
AsuII TTCGAA 1 cut(s) 822
AvaI CYCGRG 4 cut(s) 137, 143, 171, 512
AvaII GGWCC 3 cut(s) 49, 175, 442
BanII GRGCYC 2 cut(s) 144, 382
BauI CACGAG 1 cut(s) 77
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 3 cut(s) 144, 382, 915
BbvCI CCTCAGC 2 cut(s) 257, 1022
BbvI GCAGC 6 cut(s) 272, 593, 965, 1039, 1063, 1087
BccI CCATC 3 cut(s) 24, 796, 802
BceAI ACGGC 2 cut(s) 46, 931
BclI TGATCA 3 cut(s) 417, 588, 708
BcnI CCSGG 3 cut(s) 172, 173, 316
BcoDI GTCTC 2 cut(s) 140, 672
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 2 cut(s) 83, 866
BisI GCNGC 8 cut(s) 250, 286, 582, 719, 979, 1028, 1052, 1101
BlsI GCNGC 8 cut(s) 251, 287, 583, 720, 980, 1029, 1053, 1102
BmcAI AGTACT 1 cut(s) 687
Bme1390I CCNGG 3 cut(s) 172, 173, 316
Bme18I GGWCC 3 cut(s) 49, 175, 442
BmeT110I CYCGRG 4 cut(s) 137, 143, 171, 512
BmgT120I GGNCC 4 cut(s) 32, 49, 175, 442
BmiI GGNNCC 2 cut(s) 176, 177
BmrFI CCNGG 3 cut(s) 172, 173, 316
BmsI GCATC 2 cut(s) 270, 852
BpiI GAAGAC 1 cut(s) 33
Bpu10I CCTNAGC 2 cut(s) 257, 1022
Bpu14I TTCGAA 1 cut(s) 822
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 3 cut(s) 172, 173, 316
BsaBI GATNNNNATC 1 cut(s) 659
BsaHI GRCGYC 1 cut(s) 1079
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 1 cut(s) 171
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 172
Bse1I ACTGG 1 cut(s) 241
Bse3DI GCAATG 2 cut(s) 564, 576
Bse8I GATNNNNATC 1 cut(s) 659
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 3 cut(s) 113, 162, 261
BseJI GATNNNNATC 1 cut(s) 659
BseLI CCNNNNNNNGG 2 cut(s) 22, 172
BseMI GCAATG 2 cut(s) 564, 576
BseMII CTCAG 2 cut(s) 271, 1013
BseNI ACTGG 1 cut(s) 241
BsePI GCGCGC 1 cut(s) 693
BseRI GAGGAG 2 cut(s) 125, 1051
BseX3I CGGCCG 1 cut(s) 635
BseXI GCAGC 6 cut(s) 272, 593, 965, 1039, 1063, 1087
BseYI CCCAGC 1 cut(s) 198
BsgI GTGCAG 1 cut(s) 1019
Bsh1236I CGCG 9 cut(s) 37, 354, 376, 402, 665, 677, 695, 860, 1094
Bsh1285I CGRYCG 2 cut(s) 352, 638
BshFI GGCC 3 cut(s) 33, 252, 637
BsiEI CGRYCG 2 cut(s) 352, 638
BsiHKAI GWGCWC 3 cut(s) 144, 382, 915
BsiHKCI CYCGRG 4 cut(s) 137, 143, 171, 512
BsiSI CCGG 2 cut(s) 172, 316
BslFI GGGAC 2 cut(s) 188, 217
BslI CCNNNNNNNGG 2 cut(s) 22, 172
BsmAI GTCTC 2 cut(s) 140, 672
BsmBI CGTCTC 1 cut(s) 672
BsmFI GGGAC 2 cut(s) 188, 217
BsnI GGCC 3 cut(s) 33, 252, 637
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 4 cut(s) 137, 143, 171, 512
Bsp119I TTCGAA 1 cut(s) 822
Bsp1286I GDGCHC 3 cut(s) 144, 382, 915
Bsp143I GATC 9 cut(s) 345, 391, 417, 483, 588, 642, 678, 708, 732
Bsp68I TCGCGA 1 cut(s) 402
BspANI GGCC 3 cut(s) 33, 252, 637
BspCNI CTCAG 2 cut(s) 270, 1014
BspFNI CGCG 9 cut(s) 37, 354, 376, 402, 665, 677, 695, 860, 1094
BspLI GGNNCC 2 cut(s) 176, 177
BspPI GGATC 2 cut(s) 353, 386
BspT104I TTCGAA 1 cut(s) 822
BspTNI GGTCTC 1 cut(s) 140
BsrBI CCGCTC 2 cut(s) 267, 721
BsrDI GCAATG 2 cut(s) 564, 576
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 171
BssHII GCGCGC 1 cut(s) 693
BssMI GATC 9 cut(s) 345, 391, 417, 483, 588, 642, 678, 708, 732
BssNI GRCGYC 1 cut(s) 1079
BssSI CACGAG 1 cut(s) 77
Bst2BI CACGAG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 997
BstACI GRCGYC 1 cut(s) 1079
BstBI TTCGAA 1 cut(s) 822
BstC8I GCNNGC 3 cut(s) 35, 378, 695
BstDEI CTNAG 2 cut(s) 257, 1022
BstF5I GGATG 3 cut(s) 113, 162, 261
BstFNI CGCG 9 cut(s) 37, 354, 376, 402, 665, 677, 695, 860, 1094
BstHHI GCGC 3 cut(s) 454, 695, 697
BstKTI GATC 9 cut(s) 348, 394, 420, 486, 591, 645, 681, 711, 735
BstMAI GTCTC 2 cut(s) 140, 672
BstMBI GATC 9 cut(s) 345, 391, 417, 483, 588, 642, 678, 708, 732
BstMCI CGRYCG 2 cut(s) 352, 638
BstMWI GCNNNNNNNGC 9 cut(s) 128, 246, 258, 366, 581, 703, 857, 1027, 1100
BstSCI CCNGG 3 cut(s) 170, 171, 314
BstSFI CTRYAG 2 cut(s) 83, 866
BstUI CGCG 9 cut(s) 37, 354, 376, 402, 665, 677, 695, 860, 1094
BstV1I GCAGC 6 cut(s) 272, 593, 965, 1039, 1063, 1087
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BstZI CGGCCG 1 cut(s) 635
BsuRI GGCC 3 cut(s) 33, 252, 637
BtgZI GCGATG 2 cut(s) 821, 875
BtsCI GGATG 3 cut(s) 113, 162, 261
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 2 cut(s) 295, 1002
BtuMI TCGCGA 1 cut(s) 402
Cac8I GCNNGC 3 cut(s) 35, 378, 695
CfoI GCGC 3 cut(s) 454, 695, 697
Cfr13I GGNCC 4 cut(s) 32, 49, 175, 442
Cfr9I CCCGGG 1 cut(s) 171
CseI GACGC 5 cut(s) 382, 654, 866, 1068, 1100
Csp6I GTAC 1 cut(s) 686
CspCI CAANNNNNGTGG 2 cut(s) 1093, 1128
CviAII CATG 1 cut(s) 467
CviQI GTAC 1 cut(s) 686
DdeI CTNAG 2 cut(s) 257, 1022
DpnI GATC 9 cut(s) 347, 393, 419, 485, 590, 644, 680, 710, 734
DpnII GATC 9 cut(s) 345, 391, 417, 483, 588, 642, 678, 708, 732
EaeI YGGCCR 2 cut(s) 250, 635
EagI CGGCCG 1 cut(s) 635
EciI GGCGGA 4 cut(s) 109, 178, 227, 856
Ecl136II GAGCTC 2 cut(s) 142, 380
EclXI CGGCCG 1 cut(s) 635
Eco24I GRGCYC 2 cut(s) 144, 382
Eco31I GGTCTC 1 cut(s) 140
Eco47I GGWCC 3 cut(s) 49, 175, 442
Eco52I CGGCCG 1 cut(s) 635
Eco53kI GAGCTC 2 cut(s) 142, 380
Eco88I CYCGRG 4 cut(s) 137, 143, 171, 512
EcoICRI GAGCTC 2 cut(s) 142, 380
EcoO109I RGGNCCY 2 cut(s) 49, 175
EcoRI GAATTC 1 cut(s) 775
EcoT38I GRGCYC 2 cut(s) 144, 382
Esp3I CGTCTC 1 cut(s) 672
FaeI CATG 1 cut(s) 470
FaiI YATR 3 cut(s) 366, 468, 764
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FaqI GGGAC 2 cut(s) 188, 217
FatI CATG 1 cut(s) 466
FauI CCCGC 2 cut(s) 42, 1023
FbaI TGATCA 3 cut(s) 417, 588, 708
FblI GTMKAC 2 cut(s) 533, 726
Fnu4HI GCNGC 8 cut(s) 250, 286, 582, 719, 979, 1028, 1052, 1101
FokI GGATG 3 cut(s) 100, 149, 248
FriOI GRGCYC 2 cut(s) 144, 382
Fsp4HI GCNGC 8 cut(s) 250, 286, 582, 719, 979, 1028, 1052, 1101
FspBI CTAG 1 cut(s) 53
FspI TGCGCA 1 cut(s) 453
GlaI GCGC 3 cut(s) 453, 694, 696
GluI GCNGC 8 cut(s) 250, 286, 582, 719, 979, 1028, 1052, 1101
GsaI CCCAGC 1 cut(s) 202
HaeIII GGCC 3 cut(s) 33, 252, 637
HapII CCGG 2 cut(s) 172, 316
HgaI GACGC 5 cut(s) 382, 654, 866, 1068, 1100
HhaI GCGC 3 cut(s) 454, 695, 697
Hin1I GRCGYC 1 cut(s) 1079
Hin1II CATG 1 cut(s) 470
Hin6I GCGC 3 cut(s) 452, 693, 695
HinP1I GCGC 3 cut(s) 452, 693, 695
HincII GTYRAC 2 cut(s) 534, 727
HindII GTYRAC 2 cut(s) 534, 727
HindIII AAGCTT 3 cut(s) 339, 766, 887
HinfI GANTC 9 cut(s) 97, 152, 230, 292, 326, 430, 515, 530, 552
HpaII CCGG 2 cut(s) 172, 316
HphI GGTGA 3 cut(s) 496, 932, 1075
Hpy166II GTNNAC 3 cut(s) 534, 727, 993
Hpy188III TCNNGA 5 cut(s) 145, 383, 401, 487, 772
Hpy8I GTNNAC 3 cut(s) 534, 727, 993
Hpy99I CGWCG 6 cut(s) 230, 407, 643, 728, 731, 1094
HpyAV CCTTC 4 cut(s) 16, 280, 838, 940
HpyCH4III ACNGT 1 cut(s) 997
HpyCH4V TGCA 4 cut(s) 475, 581, 901, 1000
HpyF10VI GCNNNNNNNGC 9 cut(s) 128, 246, 258, 366, 581, 703, 857, 1027, 1100
HpyF3I CTNAG 2 cut(s) 257, 1022
Hsp92I GRCGYC 1 cut(s) 1079
Hsp92II CATG 1 cut(s) 470
HspAI GCGC 3 cut(s) 452, 693, 695
KflI GGGWCCC 1 cut(s) 175
Ksp22I TGATCA 3 cut(s) 417, 588, 708
Kzo9I GATC 9 cut(s) 345, 391, 417, 483, 588, 642, 678, 708, 732
LmnI GCTCC 4 cut(s) 18, 272, 910, 1130
Lsp1109I GCAGC 6 cut(s) 272, 593, 965, 1039, 1063, 1087
LweI GCATC 2 cut(s) 270, 852
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 1 cut(s) 502
MalI GATC 9 cut(s) 347, 393, 419, 485, 590, 644, 680, 710, 734
MbiI CCGCTC 2 cut(s) 267, 721
MboI GATC 9 cut(s) 345, 391, 417, 483, 588, 642, 678, 708, 732
MboII GAAGA 4 cut(s) 38, 398, 401, 794
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 3 cut(s) 144, 382, 915
MluCI AATT 4 cut(s) 333, 396, 498, 775
MlyI GAGTC 8 cut(s) 161, 239, 286, 335, 424, 524, 539, 561
MmeI TCCRAC 3 cut(s) 180, 888, 1113
MroXI GAANNNNTTC 1 cut(s) 950
MseI TTAA 3 cut(s) 126, 1047, 1141
MslI CAYNNNNRTG 1 cut(s) 918
MspA1I CMGCKG 2 cut(s) 249, 1030
MspI CCGG 2 cut(s) 172, 316
MspR9I CCNGG 3 cut(s) 172, 173, 316
MvnI CGCG 9 cut(s) 37, 354, 376, 402, 665, 677, 695, 860, 1094
MwoI GCNNNNNNNGC 9 cut(s) 128, 246, 258, 366, 581, 703, 857, 1027, 1100
NciI CCSGG 3 cut(s) 172, 173, 316
NdeII GATC 9 cut(s) 345, 391, 417, 483, 588, 642, 678, 708, 732
NlaIII CATG 1 cut(s) 470
NlaIV GGNNCC 2 cut(s) 176, 177
NmuCI GTSAC 1 cut(s) 502
NruI TCGCGA 1 cut(s) 402
NsbI TGCGCA 1 cut(s) 453
NspV TTCGAA 1 cut(s) 822
OliI CACNNNNGTG 1 cut(s) 918
PaeR7I CTCGAG 3 cut(s) 137, 143, 512
PauI GCGCGC 1 cut(s) 693
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 732
PdmI GAANNNNTTC 1 cut(s) 950
PfeI GAWTC 1 cut(s) 97
PflFI GACNNNGTC 2 cut(s) 151, 538
PkrI GCNGC 8 cut(s) 251, 287, 583, 720, 980, 1029, 1053, 1102
PleI GAGTC 8 cut(s) 160, 238, 286, 334, 424, 523, 538, 560
PpsI GAGTC 8 cut(s) 160, 238, 286, 334, 424, 523, 538, 560
PpuMI RGGWCCY 2 cut(s) 49, 175
Psp124BI GAGCTC 2 cut(s) 144, 382
Psp5II RGGWCCY 2 cut(s) 49, 175
PspFI CCCAGC 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 176, 177
PspPI GGNCC 4 cut(s) 32, 49, 175, 442
PspPPI RGGWCCY 2 cut(s) 49, 175
PspXI VCTCGAGB 2 cut(s) 137, 512
PsuI RGATCY 1 cut(s) 391
PsyI GACNNNGTC 2 cut(s) 151, 538
PteI GCGCGC 1 cut(s) 693
RruI TCGCGA 1 cut(s) 402
RsaI GTAC 1 cut(s) 687
RsaNI GTAC 1 cut(s) 686
RseI CAYNNNNRTG 1 cut(s) 918
SacI GAGCTC 2 cut(s) 144, 382
SalI GTCGAC 2 cut(s) 532, 725
SaqAI TTAA 3 cut(s) 126, 1047, 1141
SatI GCNGC 8 cut(s) 250, 286, 582, 719, 979, 1028, 1052, 1101
Sau3AI GATC 9 cut(s) 345, 391, 417, 483, 588, 642, 678, 708, 732
Sau96I GGNCC 4 cut(s) 32, 49, 175, 442
ScaI AGTACT 1 cut(s) 687
SchI GAGTC 8 cut(s) 161, 239, 286, 335, 424, 524, 539, 561
ScrFI CCNGG 3 cut(s) 172, 173, 316
SduI GDGCHC 3 cut(s) 144, 382, 915
SfaNI GCATC 2 cut(s) 270, 852
SfcI CTRYAG 2 cut(s) 83, 866
Sfr274I CTCGAG 3 cut(s) 137, 143, 512
SfuI TTCGAA 1 cut(s) 822
SgrDI CGTCGACG 1 cut(s) 725
SinI GGWCC 3 cut(s) 49, 175, 442
SlaI CTCGAG 3 cut(s) 137, 143, 512
SmaI CCCGGG 1 cut(s) 173
SmiMI CAYNNNNRTG 1 cut(s) 918
SmlI CTYRAG 4 cut(s) 44, 137, 143, 512
SmoI CTYRAG 4 cut(s) 44, 137, 143, 512
Sse9I AATT 4 cut(s) 333, 396, 498, 775
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 2 cut(s) 144, 382
StyD4I CCNGG 3 cut(s) 170, 171, 314
TaaI ACNGT 1 cut(s) 997
TaqII GACCGA 2 cut(s) 164, 459
TasI AATT 4 cut(s) 333, 396, 498, 775
TatI WGTACW 1 cut(s) 685
TauI GCSGC 2 cut(s) 252, 721
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 3 cut(s) 126, 1047, 1141
Tru9I TTAA 3 cut(s) 126, 1047, 1141
TscAI CASTG 2 cut(s) 302, 1002
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 6 cut(s) 285, 581, 978, 1027, 1051, 1100
Tsp45I GTSAC 1 cut(s) 502
TspGWI ACGGA 1 cut(s) 759
TspMI CCCGGG 1 cut(s) 171
TspRI CASTG 2 cut(s) 302, 1002
Tth111I GACNNNGTC 2 cut(s) 151, 538
VpaK11BI GGWCC 3 cut(s) 49, 175, 442
XapI RAATTY 2 cut(s) 333, 775
XhoI CTCGAG 3 cut(s) 137, 143, 512
XmaI CCCGGG 1 cut(s) 171
XmiI GTMKAC 2 cut(s) 533, 726
XmnI GAANNNNTTC 1 cut(s) 950
XspI CTAG 1 cut(s) 53
ZrmI AGTACT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.