pycom05g21450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014628
Physical Location & Seq
Reverse (-)
24088519 .. 24089772
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21450.1

Sequence Viewer

Length: 1050 bp
ATGAGGCTGGGCACGGGCCGGGCCGGGCCCAATTTTGAAGGAACCGGACCGGACCTAAGTTCAAAGGGAACGGGCCGGGCCGGGCCGGGGATTACATTTTTTAAATTAAGAACCGGACTTTTCCCGGCCCGGTTGAAAAGGGCCGGTTCGAGCCGGGGCCCCGGGGCCTTTTTTTTTTTTTTTTTTTTTTTCTTCAGAAAATCACTACAGCTTCTGATCGCCATATCCATGGAGGTGAAGCTCCATCAGAAGGAAGACGGCCCACGAGTTCTCAAGGTCCTAGAAGCTCTAAAACAAGCCTCCCACGAGCTACAGACCCACCTGATTCCCCACTCAAACTCATCCTCCGCCATTAACGCCCTCCTCGAGCTCGAGACCGAGTCCGACAACATCCTCTCCCGGGACCCACAACTCTCCGCCCTCTCCCAACACCTCGCCACCCTCAAAACCCTCGTCGAATCCCTCCGCCAGTCCAGCGGCCACCTCAGCATCCGCTCCTTGCTTACTCGCTGCTGCTCGACTCACTGCCTCTCCAAACTCGCCCGGTCTATCGAGTCCGAAATTCAAGCGTGGATCGATCGCGAGTGCTTACATAGCTTGACGCGAGCTCTGGAAGAAGACCCAATTCGCGACGAGGACGAGTTGATCGAACTACTGACTCAGTTCGAGGACCGAGTTGCGCAGGGGTTCAACCATGAGTTGCAAGATTTGATCTTGAAGTCCAAAGTTGTCACCTCGCTCGAGTCAGTTCTGTGCGAGTCGACTCGGTCATCGAAGCGAGTCAAAGAACACACTGCTTTCGCCATTGCAGCTCTGATCAAGTTCAATAAAGATGTTTTCGTGGGTCAAGTTTTGATCGGTCGGCCGACGATCAGCTCTCTGATAAAAATCGCGTCTCCGAACGCGATCAGAGTACTCTGCGCGCTCGTCAGGCTGATCAAATCGCCGCTCGTCGACGAGATCGAGTTCAACGGAGAACTACCCCAGATCATAAGCTTCCTGAATTCCGAAGATTTGGAAATGCGAGTGATGGCTATGGTCTGCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

350

Amino Acids

38.78

Weight (kDa)

8.16

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014628)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02430
malus_domestica MD02G1021100.v1.1 MD15G1164200.v1.1
prunus_persica Prupe.7G250400_v2.0.a1
pyrus_communis pycom02g01870 pycom05g21450 pycom15g14730
rosa_chinensis RchiOBHm_Chr2g0087391
rosa_laevigata RLG00000015862
rosa_multiflora Rmu_sc0006064.1_g000006
rosa_roxburghii Rroxscaffold_2G00153600
rosa_rugosa Rorug01G0474800
rosa_samantha Rh2AG028300 Rh2BG028200 Rh2CG028500 Rh2DG028300
rosa_wichuraiana Rw2G002250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 681
AccBSI CCGCTC 2 cut(s) 495, 949
AccI GTMKAC 2 cut(s) 761, 954
AccII CGCG 6 cut(s) 582, 604, 630, 893, 905, 923
AciI CCGC 6 cut(s) 348, 417, 466, 477, 493, 947
AclWI GGATC 1 cut(s) 581
AcoI YGGCCR 2 cut(s) 478, 863
AcsI RAATTY 2 cut(s) 561, 1003
AcuI CTGAAG 1 cut(s) 178
AfaI GTAC 1 cut(s) 915
AfiI CCNNNNNNNGG 3 cut(s) 87, 250, 400
AgsI TTSAA 8 cut(s) 38, 63, 136, 566, 691, 718, 826, 970
Alw21I GWGCWC 2 cut(s) 372, 610
Alw26I GTCTC 2 cut(s) 368, 900
AlwI GGATC 1 cut(s) 581
AlwNI CAGNNNCTG 1 cut(s) 214
Ama87I CYCGRG 5 cut(s) 161, 365, 371, 399, 740
ApaI GGGCCC 2 cut(s) 30, 161
ApeKI GCWGC 3 cut(s) 510, 513, 809
ApoI RAATTY 2 cut(s) 561, 1003
ArsI GACNNNNNNTTYG 2 cut(s) 438, 470
AspLEI GCGC 3 cut(s) 682, 923, 925
AsuHPI GGTGA 2 cut(s) 247, 724
AvaI CYCGRG 5 cut(s) 161, 365, 371, 399, 740
AvaII GGWCC 5 cut(s) 47, 52, 277, 403, 670
BaeGI GKGCMC 3 cut(s) 14, 30, 161
BanII GRGCYC 4 cut(s) 30, 161, 372, 610
BauI CACGAG 2 cut(s) 264, 305
BbsI GAAGAC 2 cut(s) 261, 624
Bbv12I GWGCWC 2 cut(s) 372, 610
BbvCI CCTCAGC 1 cut(s) 485
BbvI GCAGC 3 cut(s) 497, 500, 821
BccI CCATC 2 cut(s) 252, 1024
BceAI ACGGC 1 cut(s) 274
BclI TGATCA 2 cut(s) 816, 936
BcoDI GTCTC 2 cut(s) 368, 900
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 2 cut(s) 206, 311
BisI GCNGC 5 cut(s) 478, 511, 514, 810, 947
BlsI GCNGC 5 cut(s) 479, 512, 515, 811, 948
BmcAI AGTACT 1 cut(s) 915
Bme18I GGWCC 5 cut(s) 47, 52, 277, 403, 670
BmeT110I CYCGRG 5 cut(s) 161, 365, 371, 399, 740
BmiI GGNNCC 8 cut(s) 28, 43, 158, 159, 160, 166, 404, 405
BmsI GCATC 1 cut(s) 498
BpiI GAAGAC 2 cut(s) 261, 624
Bpu10I CCTNAGC 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 257
Bsa29I ATCGAT 1 cut(s) 576
BsaBI GATNNNNATC 1 cut(s) 887
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 7 cut(s) 86, 154, 160, 161, 162, 228, 399
BsaWI WCCGGW 3 cut(s) 44, 49, 113
BsaXI ACNNNNNCTCC 6 cut(s) 329, 359, 380, 410, 515, 545
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 250, 400
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 1 cut(s) 469
Bse3DI GCAATG 1 cut(s) 804
Bse8I GATNNNNATC 1 cut(s) 887
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 7 cut(s) 86, 154, 160, 161, 162, 228, 399
BseGI GGATG 3 cut(s) 341, 390, 489
BseJI GATNNNNATC 1 cut(s) 887
BseLI CCNNNNNNNGG 3 cut(s) 87, 250, 400
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 2 cut(s) 499, 674
BseNI ACTGG 1 cut(s) 469
BsePI GCGCGC 1 cut(s) 921
BseRI GAGGAG 1 cut(s) 353
BseSI GKGCMC 3 cut(s) 14, 30, 161
BseX3I CGGCCG 1 cut(s) 863
BseXI GCAGC 3 cut(s) 497, 500, 821
BseYI CCCAGC 1 cut(s) 7
Bsh1236I CGCG 6 cut(s) 582, 604, 630, 893, 905, 923
Bsh1285I CGRYCG 3 cut(s) 580, 862, 866
BshVI ATCGAT 1 cut(s) 576
BsiEI CGRYCG 3 cut(s) 580, 862, 866
BsiHKAI GWGCWC 2 cut(s) 372, 610
BsiHKCI CYCGRG 5 cut(s) 161, 365, 371, 399, 740
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 3 cut(s) 87, 250, 400
BsmAI GTCTC 2 cut(s) 368, 900
BsmBI CGTCTC 1 cut(s) 900
BsmFI GGGAC 1 cut(s) 416
Bso31I GGTCTC 1 cut(s) 368
BsoBI CYCGRG 5 cut(s) 161, 365, 371, 399, 740
Bsp120I GGGCCC 2 cut(s) 26, 157
Bsp1286I GDGCHC 5 cut(s) 14, 30, 161, 372, 610
Bsp19I CCATGG 1 cut(s) 228
Bsp68I TCGCGA 2 cut(s) 582, 630
BspACI CCGC 6 cut(s) 348, 417, 466, 477, 493, 947
BspCNI CTCAG 2 cut(s) 498, 673
BspDI ATCGAT 1 cut(s) 576
BspFNI CGCG 6 cut(s) 582, 604, 630, 893, 905, 923
BspLI GGNNCC 8 cut(s) 28, 43, 158, 159, 160, 166, 404, 405
BspPI GGATC 1 cut(s) 581
BspTNI GGTCTC 1 cut(s) 368
BsrBI CCGCTC 2 cut(s) 495, 949
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 1 cut(s) 469
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 7 cut(s) 86, 154, 160, 161, 162, 228, 399
BssHII GCGCGC 1 cut(s) 921
BssSI CACGAG 2 cut(s) 264, 305
BssT1I CCWWGG 1 cut(s) 228
Bst2BI CACGAG 2 cut(s) 264, 305
BstC8I GCNNGC 2 cut(s) 606, 923
BstDEI CTNAG 3 cut(s) 56, 485, 660
BstDSI CCRYGG 1 cut(s) 228
BstF5I GGATG 3 cut(s) 341, 390, 489
BstFNI CGCG 6 cut(s) 582, 604, 630, 893, 905, 923
BstHHI GCGC 3 cut(s) 682, 923, 925
BstMAI GTCTC 2 cut(s) 368, 900
BstMCI CGRYCG 3 cut(s) 580, 862, 866
BstMWI GCNNNNNNNGC 6 cut(s) 356, 474, 486, 594, 809, 931
BstSFI CTRYAG 2 cut(s) 206, 311
BstSLI GKGCMC 3 cut(s) 14, 30, 161
BstUI CGCG 6 cut(s) 582, 604, 630, 893, 905, 923
BstV1I GCAGC 3 cut(s) 497, 500, 821
BstV2I GAAGAC 2 cut(s) 261, 624
BstXI CCANNNNNNTGG 1 cut(s) 229
BstZI CGGCCG 1 cut(s) 863
Bsu15I ATCGAT 1 cut(s) 576
BsuTUI ATCGAT 1 cut(s) 576
BtgI CCRYGG 1 cut(s) 228
BtsCI GGATG 3 cut(s) 341, 390, 489
BtsI GCAGTG 2 cut(s) 523, 792
BtsIMutI CAGTG 2 cut(s) 523, 792
BtuMI TCGCGA 2 cut(s) 582, 630
Cac8I GCNNGC 2 cut(s) 606, 923
CaiI CAGNNNCTG 1 cut(s) 214
CfoI GCGC 3 cut(s) 682, 923, 925
Cfr10I RCCGGY 1 cut(s) 143
Cfr9I CCCGGG 2 cut(s) 161, 399
ClaI ATCGAT 1 cut(s) 576
CpoI CGGWCCG 1 cut(s) 47
CseI GACGC 2 cut(s) 610, 882
Csp6I GTAC 1 cut(s) 914
CspI CGGWCCG 1 cut(s) 47
CviAII CATG 2 cut(s) 229, 695
CviQI GTAC 1 cut(s) 914
DdeI CTNAG 3 cut(s) 56, 485, 660
DraI TTTAAA 1 cut(s) 103
EaeI YGGCCR 2 cut(s) 478, 863
EagI CGGCCG 1 cut(s) 863
EciI GGCGGA 3 cut(s) 337, 406, 455
Ecl136II GAGCTC 2 cut(s) 370, 608
EclXI CGGCCG 1 cut(s) 863
Eco130I CCWWGG 1 cut(s) 228
Eco24I GRGCYC 4 cut(s) 30, 161, 372, 610
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 5 cut(s) 47, 52, 277, 403, 670
Eco52I CGGCCG 1 cut(s) 863
Eco53kI GAGCTC 2 cut(s) 370, 608
Eco57I CTGAAG 1 cut(s) 178
Eco88I CYCGRG 5 cut(s) 161, 365, 371, 399, 740
EcoICRI GAGCTC 2 cut(s) 370, 608
EcoO109I RGGNCCY 5 cut(s) 157, 158, 165, 277, 403
EcoRI GAATTC 1 cut(s) 1003
EcoT14I CCWWGG 1 cut(s) 228
EcoT38I GRGCYC 4 cut(s) 30, 161, 372, 610
ErhI CCWWGG 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 900
FaeI CATG 2 cut(s) 232, 698
FaiI YATR 6 cut(s) 224, 230, 594, 696, 992, 1037
FaqI GGGAC 1 cut(s) 416
FatI CATG 2 cut(s) 228, 694
FbaI TGATCA 2 cut(s) 816, 936
FblI GTMKAC 2 cut(s) 761, 954
Fnu4HI GCNGC 5 cut(s) 478, 511, 514, 810, 947
FokI GGATG 3 cut(s) 328, 377, 476
FriOI GRGCYC 4 cut(s) 30, 161, 372, 610
Fsp4HI GCNGC 5 cut(s) 478, 511, 514, 810, 947
FspBI CTAG 1 cut(s) 281
FspI TGCGCA 1 cut(s) 681
GlaI GCGC 3 cut(s) 681, 922, 924
GluI GCNGC 5 cut(s) 478, 511, 514, 810, 947
GsaI CCCAGC 1 cut(s) 11
HgaI GACGC 2 cut(s) 610, 882
HhaI GCGC 3 cut(s) 682, 923, 925
Hin1II CATG 2 cut(s) 232, 698
Hin6I GCGC 3 cut(s) 680, 921, 923
HinP1I GCGC 3 cut(s) 680, 921, 923
HincII GTYRAC 2 cut(s) 762, 955
HindII GTYRAC 2 cut(s) 762, 955
HindIII AAGCTT 1 cut(s) 994
HphI GGTGA 2 cut(s) 247, 724
Hpy166II GTNNAC 2 cut(s) 762, 955
Hpy188III TCNNGA 6 cut(s) 373, 581, 611, 629, 715, 1000
Hpy8I GTNNAC 2 cut(s) 762, 955
Hpy99I CGWCG 5 cut(s) 458, 635, 871, 956, 959
HpyAV CCTTC 2 cut(s) 32, 244
HpyCH4V TGCA 2 cut(s) 703, 809
HpyF10VI GCNNNNNNNGC 6 cut(s) 356, 474, 486, 594, 809, 931
HpyF3I CTNAG 3 cut(s) 56, 485, 660
Hsp92II CATG 2 cut(s) 232, 698
HspAI GCGC 3 cut(s) 680, 921, 923
KflI GGGWCCC 1 cut(s) 403
Ksp22I TGATCA 2 cut(s) 816, 936
LmnI GCTCC 2 cut(s) 246, 500
Lsp1109I GCAGC 3 cut(s) 497, 500, 821
LweI GCATC 1 cut(s) 498
MaeI CTAG 1 cut(s) 281
MaeIII GTNAC 1 cut(s) 730
MbiI CCGCTC 2 cut(s) 495, 949
MboII GAAGA 5 cut(s) 184, 266, 626, 629, 1022
MhlI GDGCHC 5 cut(s) 14, 30, 161, 372, 610
MluCI AATT 5 cut(s) 31, 104, 561, 624, 1003
MlyI GAGTC 8 cut(s) 389, 514, 563, 652, 752, 757, 767, 789
MmeI TCCRAC 1 cut(s) 408
MseI TTAA 3 cut(s) 102, 107, 354
MslI CAYNNNNRTG 2 cut(s) 227, 233
MspA1I CMGCKG 1 cut(s) 477
MvnI CGCG 6 cut(s) 582, 604, 630, 893, 905, 923
MwoI GCNNNNNNNGC 6 cut(s) 356, 474, 486, 594, 809, 931
NcoI CCATGG 1 cut(s) 228
NlaIII CATG 2 cut(s) 232, 698
NlaIV GGNNCC 8 cut(s) 28, 43, 158, 159, 160, 166, 404, 405
NmuCI GTSAC 1 cut(s) 730
NruI TCGCGA 2 cut(s) 582, 630
NsbI TGCGCA 1 cut(s) 681
PaeR7I CTCGAG 3 cut(s) 365, 371, 740
PauI GCGCGC 1 cut(s) 921
PcsI WCGNNNNNNNCGW 3 cut(s) 363, 645, 960
PfeI GAWTC 2 cut(s) 325, 458
PflFI GACNNNGTC 2 cut(s) 379, 766
PkrI GCNGC 5 cut(s) 479, 512, 515, 811, 948
Ple19I CGATCG 1 cut(s) 580
PleI GAGTC 8 cut(s) 388, 514, 562, 652, 751, 757, 766, 788
PpsI GAGTC 8 cut(s) 388, 514, 562, 652, 751, 757, 766, 788
PpuMI RGGWCCY 2 cut(s) 277, 403
Psp124BI GAGCTC 2 cut(s) 372, 610
Psp5II RGGWCCY 2 cut(s) 277, 403
PspFI CCCAGC 1 cut(s) 7
PspN4I GGNNCC 8 cut(s) 28, 43, 158, 159, 160, 166, 404, 405
PspOMI GGGCCC 2 cut(s) 26, 157
PspPPI RGGWCCY 2 cut(s) 277, 403
PspXI VCTCGAGB 2 cut(s) 365, 740
PstNI CAGNNNCTG 1 cut(s) 214
PsyI GACNNNGTC 2 cut(s) 379, 766
PteI GCGCGC 1 cut(s) 921
PvuI CGATCG 1 cut(s) 580
RruI TCGCGA 2 cut(s) 582, 630
RsaI GTAC 1 cut(s) 915
RsaNI GTAC 1 cut(s) 914
RseI CAYNNNNRTG 2 cut(s) 227, 233
Rsr2I CGGWCCG 1 cut(s) 47
RsrII CGGWCCG 1 cut(s) 47
SacI GAGCTC 2 cut(s) 372, 610
SalI GTCGAC 2 cut(s) 760, 953
SaqAI TTAA 3 cut(s) 102, 107, 354
SatI GCNGC 5 cut(s) 478, 511, 514, 810, 947
ScaI AGTACT 1 cut(s) 915
SchI GAGTC 8 cut(s) 389, 514, 563, 652, 752, 757, 767, 789
SduI GDGCHC 5 cut(s) 14, 30, 161, 372, 610
SfaNI GCATC 1 cut(s) 498
SfcI CTRYAG 2 cut(s) 206, 311
Sfr274I CTCGAG 3 cut(s) 365, 371, 740
SgrDI CGTCGACG 1 cut(s) 953
SinI GGWCC 5 cut(s) 47, 52, 277, 403, 670
SlaI CTCGAG 3 cut(s) 365, 371, 740
SmaI CCCGGG 2 cut(s) 163, 401
SmiMI CAYNNNNRTG 2 cut(s) 227, 233
SmlI CTYRAG 4 cut(s) 272, 365, 371, 740
SmoI CTYRAG 4 cut(s) 272, 365, 371, 740
Sse9I AATT 5 cut(s) 31, 104, 561, 624, 1003
SsiI CCGC 6 cut(s) 348, 417, 466, 477, 493, 947
SspMI CTAG 1 cut(s) 281
SstI GAGCTC 2 cut(s) 372, 610
StyI CCWWGG 1 cut(s) 228
TaqII GACCGA 4 cut(s) 392, 687, 756, 848
TasI AATT 5 cut(s) 31, 104, 561, 624, 1003
TatI WGTACW 1 cut(s) 913
TauI GCSGC 2 cut(s) 480, 949
TfiI GAWTC 2 cut(s) 325, 458
Tru1I TTAA 3 cut(s) 102, 107, 354
Tru9I TTAA 3 cut(s) 102, 107, 354
TscAI CASTG 2 cut(s) 530, 799
TseFI GTSAC 1 cut(s) 730
TseI GCWGC 3 cut(s) 510, 513, 809
Tsp45I GTSAC 1 cut(s) 730
TspGWI ACGGA 1 cut(s) 987
TspMI CCCGGG 2 cut(s) 161, 399
TspRI CASTG 2 cut(s) 530, 799
Tth111I GACNNNGTC 2 cut(s) 379, 766
VpaK11BI GGWCC 5 cut(s) 47, 52, 277, 403, 670
XapI RAATTY 2 cut(s) 561, 1003
XhoI CTCGAG 3 cut(s) 365, 371, 740
XmaI CCCGGG 2 cut(s) 161, 399
XmiI GTMKAC 2 cut(s) 761, 954
XspI CTAG 1 cut(s) 281
ZrmI AGTACT 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.