Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0015514

Physical Location & Seq

Genomic Coordinates Reverse (-)

2760168 .. 2763262

Gene Structure 3095 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1036400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2313 bp

ATGAAGACATCGAGAACATTTTCTCTTCTGTTGTGTACTTTCACCTGCTTCTTTGTGCGTTTATCTCATGCATCGGACACCCTGAGTCATGGACAATTCTTGTACAGCAATCAAACCTTGGTTTCCGCCGGCGGAGTGTTTGAGCTAGGCTTTTTCAGCTCCCCCTCTTCGCCAAACACGTACTTGGGAATCTGGTTCAAGAATGACAAGAACCAAAAGCCAGTTTGGGTTGCAAACAGAAACAGCCCCCTCATGGATTCCGTTGGAACGGGGGGTTCCTCCGAAGTATATCTCACCATCCGGTATGATGGGAACATGGTGATCCTTGACAGAAGAGGCACTCCATTTATTGTGAATTATGGAGCAGTTGCACCAGGGAAAAACACAACTGCAACGATTCTTGACTCGGGAAATCTTGTTCTGATGGAAGGAGGAGACACGTTTTGGCAGAGTTTCTATTACCCGACGGATACATTTCTTCCTGGAATGAAGTTGGGGTTGTTCAACATAAACACTGAGGAGTCGAGGATACAGTATCTTGTGTCCTGGTCAAGCACTTCTGACCCTACTGATGGTCCTTATGCTCTAGGCATTGATCGAGTGAACCACACTCAGTTCAACGTCTGGCGCAGCGATGGAGCTTACCAACAGATTGGATTTTGGGATGGCCATGAATTCCATTTATTCTTCAAAAGCAAGTCGGATAATCTCAACTACAGTTTCGTTTGGCATAGTAACGAGATTTATCTTACTTTTAGCAATAAAAATAGTAGCATGCCTTCATGGTTCGTGGTAGCCTCGGATGGGCTGATTGATGAGTTCATGATGGTAGGAAATGAGATTACGATTGTTGATTATTCACTATGCAATGACACAATGGCACGCAGTTCTAATGGCTGTTTGGTGCGGACGCCATCATCTTGCTCAGATGGAGATAATTTCTCACGTGTCACAGGAATGCTGCCGAGTTCATTGTTTTTAAGTTGGCCAATTCGGATAGATCCCTATGACTGTGAGATACTGTGCAGGAACAATTGCTCATGTGTTGCATATACATATGATTCCTTGCCGGATGATGGAACTCTATGTCAACTTTATTATGGAAATAAAACCGATCTTCAAAAAATCGTCAGGACAGGAAACAGCACCATCTATGTGCGTACTAACGCCTCTAAATCCGGAATTTATGGATTCCGCAGTGGCACGAGAGATAGTATGAGGCTATTACTGTCGCAGTTGAGTGCCGATGATAATGCCAGCGGTCCAAATCTTATAGAATTTGGAAAGCAGAAGGATCATGAGTTGCCACTGCTTAGCTTTTCATTCATAGTGAAATCCACTAATAATTTCTCTCTTGCAAATAAGCTTGGGCAAGGTGGTTTCGGGCCTGTTTATAAGGGCAAATTACAACAACATGACATTGCAGTCAAAAGGCTATCCAAAAGTTCTGGACAAGGACCGGTGGAGTTCAAGAACGAGGTGCAGTTAATCTCTAAGCTCCAGCACAGAAGTCTTGTGAAGCTTTTGGGTTGTTGCATTCATCGAGAAGAAAAGATACTAATATACGAGTACATGCCCAATAAAAGCTTGGATTCCTTCCTTTTCGATCAATCAAGGCGAAGGCTTTTAAATTGGAGACAGCGCATACACATAATCGAAGGGATTGCTCAAGGGCTTCTTTATCTTCACAAGTACTCTAGATTAAGAATTATTCATCGAGATCTGAAAATTAGCAACATCTTATTGGATGCTCACATGAACCCCAAGATTTCAGACTTTGGCTTGGCTAGAATTTTATCTGGGAATGAATGTCGAGCCAAAACAAATCGGGTTGTTGGAACATTTGGCTACATGTCTCCTGAGTATTTGATGCACGGCCTTTTCTCAACTAAATCCGATGTGTTCAGCTTTGGAGTCATTGTATTGGAGATTGTAAGCGGTAGGAAGAACGCAAATTTTTATGAGTCAGATCATTCCCTGAACTTACTAGGACATGCTTGGAACTTATGGAAATCTGGGCAAGCGGCGGAGCTTATGGATTCGACATTGGCAGATTCAAGTTCAATGAAAAGCCTTGTGCTCTGCATTCAGGTGGGTCTTTTATGTGTCCAAGACTGTGCAGAAGATAGGCCAACCATGTCGGATGTTGTTTCGATGTTGAGCAATGAAGGAGCAACTTTACCTAACCCTAAGCAGCCTACGTATTCGAATTTAACGACAGAGGTTGATAAACCATTAATGGTTGACAATCAACCATTAAATCGATTGCCTTCTGTAAATCTATTAACATTTTCGGAAGTAGAAGCACGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

771

Amino Acids

86.35

Weight (kDa)

6.98

Isoelectric Point (pI)

41.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	75 - 183	2.2e-22	D-mannose binding lectin
PAN_2	PF08276	308 - 369	2.6e-06	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	452 - 719	6.7e-48	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	452 - 717	1.3e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015514)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g03780
malus_domestica	MD02G1036400.v1.1 MD15G1176600.v1.1
prunus_persica	Prupe.7G239000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0089191
rosa_laevigata	RLG00000016003
rosa_multiflora	Rmu_sc0008322.1_g000027 Rmu_sc0008909.1_g000009
rosa_roxburghii	Rroxscaffold_2G00152160
rosa_rugosa	Rorug01G0488300
rosa_samantha	Rh2AG043900 Rh2BG042600 Rh2CG044100 Rh2DG042900
rosa_wichuraiana	Rw2G003920 Rw2G003930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1395
AarI	CACCTGC	1 cut(s)	53
AasI	GACNNNNNNGTC	1 cut(s)	1424
Acc36I	ACCTGC	1 cut(s)	53
AccIII	TCCGGA	1 cut(s)	1178
AciI	CCGC	8 cut(s)	126, 132, 907, 1195, 1260, 1938, 2024, 2027
AclWI	GGATC	3 cut(s)	316, 995, 1302
AcoI	YGGCCR	2 cut(s)	667, 986
AcsI	RAATTY	6 cut(s)	674, 1182, 1277, 1791, 1954, 2209
AcvI	CACGTG	1 cut(s)	947
AcyI	GRCGYC	1 cut(s)	911
AfaI	GTAC	6 cut(s)	37, 104, 182, 1162, 1571, 1694
AfiI	CCNNNNNNNGG	4 cut(s)	253, 572, 804, 1076
AflIII	ACRYGT	4 cut(s)	177, 438, 946, 1851
AgeI	ACCGGT	1 cut(s)	1459
AgsI	TTSAA	8 cut(s)	199, 505, 619, 691, 1121, 1471, 2058, 2064
AjnI	CCWGG	3 cut(s)	373, 481, 545
AloI	GAACNNNNNNTCC	2 cut(s)	402, 434
Alw21I	GWGCWC	1 cut(s)	2082
Alw26I	GTCTC	3 cut(s)	429, 1630, 1860
AlwI	GGATC	3 cut(s)	316, 995, 1302
Ama87I	CYCGRG	1 cut(s)	406
Aor13HI	TCCGGA	1 cut(s)	1178
AoxI	GGCC	5 cut(s)	667, 986, 1385, 1876, 2129
ApeKI	GCWGC	3 cut(s)	630, 961, 2194
ApoI	RAATTY	6 cut(s)	674, 1182, 1277, 1791, 1954, 2209
AseI	ATTAAT	1 cut(s)	2237
AsiGI	ACCGGT	1 cut(s)	1459
Asp700I	GAANNNNTTC	1 cut(s)	19
AspLEI	GCGC	2 cut(s)	630, 1644
AspS9I	GGNCC	4 cut(s)	575, 1262, 1385, 1457
AsuHPI	GGTGA	3 cut(s)	34, 286, 331
AsuII	TTCGAA	1 cut(s)	2207
AvaI	CYCGRG	1 cut(s)	406
AvaII	GGWCC	3 cut(s)	575, 1262, 1457
BalI	TGGCCA	2 cut(s)	669, 988
BarI	GAAGNNNNNNTAC	4 cut(s)	763, 795, 1539, 1571
BauI	CACGAG	1 cut(s)	1204
BbrPI	CACGTG	1 cut(s)	947
BbsI	GAAGAC	1 cut(s)	11
Bbv12I	GWGCWC	1 cut(s)	2082
BbvI	GCAGC	3 cut(s)	642, 948, 2206
BceAI	ACGGC	1 cut(s)	1891
BcgI	CGANNNNNNTGC	4 cut(s)	1235, 1269, 1646, 1680
BciT130I	CCWGG	3 cut(s)	375, 483, 547
BciVI	GTATCC	2 cut(s)	463, 522
BcoDI	GTCTC	3 cut(s)	429, 1630, 1860
BfaI	CTAG	5 cut(s)	146, 587, 1698, 1788, 1988
BfmI	CTRYAG	1 cut(s)	715
BfuAI	ACCTGC	1 cut(s)	53
BfuI	GTATCC	2 cut(s)	463, 522
BglII	AGATCT	1 cut(s)	1720
BisI	GCNGC	4 cut(s)	631, 962, 2025, 2195
BlpI	GCTNAGC	1 cut(s)	1313
BlsI	GCNGC	4 cut(s)	632, 963, 2026, 2196
BmcAI	AGTACT	1 cut(s)	1694
Bme1390I	CCNGG	3 cut(s)	375, 483, 547
Bme18I	GGWCC	3 cut(s)	575, 1262, 1457
BmeT110I	CYCGRG	1 cut(s)	406
BmgT120I	GGNCC	4 cut(s)	575, 1262, 1385, 1457
BmiI	GGNNCC	1 cut(s)	277
BmrFI	CCNGG	3 cut(s)	375, 483, 547
BmsI	GCATC	3 cut(s)	80, 1738, 1860
BpiI	GAAGAC	1 cut(s)	11
BpmI	CTGGAG	1 cut(s)	1484
Bpu10I	CCTNAGC	1 cut(s)	2190
Bpu1102I	GCTNAGC	1 cut(s)	1313
Bpu14I	TTCGAA	1 cut(s)	2207
BpuEI	CTTGAG	1 cut(s)	1653
Bsa29I	ATCGAT	1 cut(s)	2263
BsaAI	YACGTR	3 cut(s)	180, 947, 2202
BsaHI	GRCGYC	1 cut(s)	911
BsaJI	CCNNGG	3 cut(s)	117, 374, 798
BsaWI	WCCGGW	3 cut(s)	300, 1178, 1459
BsaXI	ACNNNNNCTCC	2 cut(s)	1905, 1935
Bsc4I	CCNNNNNNNGG	4 cut(s)	253, 572, 804, 1076
Bse118I	RCCGGY	2 cut(s)	128, 1459
Bse1I	ACTGG	1 cut(s)	221
Bse3DI	GCAATG	3 cut(s)	874, 1419, 2170
BseAI	TCCGGA	1 cut(s)	1178
BseBI	CCWGG	3 cut(s)	375, 483, 547
BseCI	ATCGAT	1 cut(s)	2263
BseDI	CCNNGG	3 cut(s)	117, 374, 798
BseGI	GGATG	6 cut(s)	297, 670, 808, 1078, 1753, 2149
BseLI	CCNNNNNNNGG	4 cut(s)	253, 572, 804, 1076
BseMI	GCAATG	3 cut(s)	874, 1419, 2170
BseMII	CTCAG	5 cut(s)	74, 507, 626, 939, 1851
BseNI	ACTGG	1 cut(s)	221
BseRI	GAGGAG	2 cut(s)	447, 533
BseXI	GCAGC	3 cut(s)	642, 948, 2206
BsgI	GTGCAG	3 cut(s)	1045, 1502, 2139
BshFI	GGCC	5 cut(s)	669, 988, 1387, 1878, 2131
BshTI	ACCGGT	1 cut(s)	1459
BshVI	ATCGAT	1 cut(s)	2263
BsiHKAI	GWGCWC	1 cut(s)	2082
BsiHKCI	CYCGRG	1 cut(s)	406
BsiSI	CCGG	5 cut(s)	129, 301, 1070, 1179, 1460
BslI	CCNNNNNNNGG	4 cut(s)	253, 572, 804, 1076
BsmAI	GTCTC	3 cut(s)	429, 1630, 1860
BsmI	GAATGC	3 cut(s)	963, 1536, 2085
BsnI	GGCC	5 cut(s)	669, 988, 1387, 1878, 2131
BsoBI	CYCGRG	1 cut(s)	406
Bsp119I	TTCGAA	1 cut(s)	2207
Bsp1286I	GDGCHC	1 cut(s)	2082
Bsp13I	TCCGGA	1 cut(s)	1178
Bsp1407I	TGTACA	1 cut(s)	102
Bsp143I	GATC	8 cut(s)	321, 595, 1000, 1114, 1294, 1606, 1720, 1969
Bsp1720I	GCTNAGC	1 cut(s)	1313
BspACI	CCGC	8 cut(s)	126, 132, 907, 1195, 1260, 1938, 2024, 2027
BspANI	GGCC	5 cut(s)	669, 988, 1387, 1878, 2131
BspCNI	CTCAG	5 cut(s)	75, 508, 625, 938, 1852
BspDI	ATCGAT	1 cut(s)	2263
BspEI	TCCGGA	1 cut(s)	1178
BspHI	TCATGA	2 cut(s)	822, 1297
BspLI	GGNNCC	1 cut(s)	277
BspMI	ACCTGC	1 cut(s)	53
BspPI	GGATC	3 cut(s)	316, 995, 1302
BspT104I	TTCGAA	1 cut(s)	2207
BsrDI	GCAATG	3 cut(s)	874, 1419, 2170
BsrFI	RCCGGY	2 cut(s)	128, 1459
BsrGI	TGTACA	1 cut(s)	102
BsrI	ACTGG	1 cut(s)	221
BssAI	RCCGGY	2 cut(s)	128, 1459
BssECI	CCNNGG	3 cut(s)	117, 374, 798
BssMI	GATC	8 cut(s)	321, 595, 1000, 1114, 1294, 1606, 1720, 1969
BssNI	GRCGYC	1 cut(s)	911
BssSI	CACGAG	1 cut(s)	1204
BssT1I	CCWWGG	1 cut(s)	117
Bst2BI	CACGAG	1 cut(s)	1204
Bst2UI	CCWGG	3 cut(s)	375, 483, 547
Bst4CI	ACNGT	6 cut(s)	534, 719, 1013, 1023, 1230, 2117
Bst6I	CTCTTC	3 cut(s)	30, 172, 328
BstACI	GRCGYC	1 cut(s)	911
BstAUI	TGTACA	1 cut(s)	102
BstBAI	YACGTR	3 cut(s)	180, 947, 2202
BstBI	TTCGAA	1 cut(s)	2207
BstC8I	GCNNGC	5 cut(s)	130, 776, 883, 1258, 2022
BstDEI	CTNAG	8 cut(s)	83, 516, 612, 925, 1313, 1494, 1860, 2190
BstF5I	GGATG	6 cut(s)	297, 670, 808, 1078, 1753, 2149
BstHHI	GCGC	2 cut(s)	630, 1644
BstKTI	GATC	8 cut(s)	324, 598, 1003, 1117, 1297, 1609, 1723, 1972
BstMAI	GTCTC	3 cut(s)	429, 1630, 1860
BstMBI	GATC	8 cut(s)	321, 595, 1000, 1114, 1294, 1606, 1720, 1969
BstMWI	GCNNNNNNNGC	1 cut(s)	156
BstNI	CCWGG	3 cut(s)	375, 483, 547
BstNSI	RCATGY	4 cut(s)	778, 1576, 1855, 1997
BstSCI	CCNGG	3 cut(s)	373, 481, 545
BstSFI	CTRYAG	1 cut(s)	715
BstSNI	TACGTA	1 cut(s)	2202
BstV1I	GCAGC	3 cut(s)	642, 948, 2206
BstV2I	GAAGAC	1 cut(s)	11
BstX2I	RGATCY	2 cut(s)	1000, 1720
BstXI	CCANNNNNNTGG	1 cut(s)	653
BstYI	RGATCY	2 cut(s)	1000, 1720
Bsu15I	ATCGAT	1 cut(s)	2263
BsuI	GTATCC	2 cut(s)	463, 522
BsuRI	GGCC	5 cut(s)	669, 988, 1387, 1878, 2131
BsuTUI	ATCGAT	1 cut(s)	2263
BtgZI	GCGATG	1 cut(s)	648
BtsCI	GGATG	6 cut(s)	297, 670, 808, 1078, 1753, 2149
BtsI	GCAGTG	2 cut(s)	1204, 1307
BtsIMutI	CAGTG	3 cut(s)	513, 1204, 1307
BveI	ACCTGC	1 cut(s)	53
Cac8I	GCNNGC	5 cut(s)	130, 776, 883, 1258, 2022
CciI	TCATGA	2 cut(s)	822, 1297
CfoI	GCGC	2 cut(s)	630, 1644
Cfr10I	RCCGGY	2 cut(s)	128, 1459
Cfr13I	GGNCC	4 cut(s)	575, 1262, 1385, 1457
ClaI	ATCGAT	1 cut(s)	2263
CseI	GACGC	1 cut(s)	919
Csp6I	GTAC	6 cut(s)	36, 103, 181, 1161, 1570, 1693
CspAI	ACCGGT	1 cut(s)	1459
CviQI	GTAC	6 cut(s)	36, 103, 181, 1161, 1570, 1693
DdeI	CTNAG	8 cut(s)	83, 516, 612, 925, 1313, 1494, 1860, 2190
DpnI	GATC	8 cut(s)	323, 597, 1002, 1116, 1296, 1608, 1722, 1971
DpnII	GATC	8 cut(s)	321, 595, 1000, 1114, 1294, 1606, 1720, 1969
DraI	TTTAAA	1 cut(s)	1629
DrdI	GACNNNNNNGTC	1 cut(s)	1424
DseDI	GACNNNNNNGTC	1 cut(s)	1424
EaeI	YGGCCR	2 cut(s)	667, 986
Eam1104I	CTCTTC	3 cut(s)	30, 172, 328
EarI	CTCTTC	3 cut(s)	30, 172, 328
EciI	GGCGGA	3 cut(s)	115, 147, 2042
Eco105I	TACGTA	1 cut(s)	2202
Eco130I	CCWWGG	1 cut(s)	117
Eco47I	GGWCC	3 cut(s)	575, 1262, 1457
Eco72I	CACGTG	1 cut(s)	947
Eco88I	CYCGRG	1 cut(s)	406
EcoRI	GAATTC	1 cut(s)	674
EcoRII	CCWGG	3 cut(s)	373, 481, 545
EcoT14I	CCWWGG	1 cut(s)	117
EcoT22I	ATGCAT	1 cut(s)	73
ErhI	CCWWGG	1 cut(s)	117
FalI	AAGNNNNNCTT	2 cut(s)	1662, 1694
FauNDI	CATATG	1 cut(s)	1057
Fnu4HI	GCNGC	4 cut(s)	631, 962, 2025, 2195
FokI	GGATG	6 cut(s)	284, 677, 815, 1085, 1760, 2156
Fsp4HI	GCNGC	4 cut(s)	631, 962, 2025, 2195
FspBI	CTAG	5 cut(s)	146, 587, 1698, 1788, 1988
GlaI	GCGC	2 cut(s)	629, 1643
GluI	GCNGC	4 cut(s)	631, 962, 2025, 2195
GsuI	CTGGAG	1 cut(s)	1484
HaeIII	GGCC	5 cut(s)	669, 988, 1387, 1878, 2131
HapII	CCGG	5 cut(s)	129, 301, 1070, 1179, 1460
HgaI	GACGC	1 cut(s)	919
HhaI	GCGC	2 cut(s)	630, 1644
Hin1I	GRCGYC	1 cut(s)	911
Hin6I	GCGC	2 cut(s)	628, 1642
HinP1I	GCGC	2 cut(s)	628, 1642
HincII	GTYRAC	2 cut(s)	1091, 2245
HindII	GTYRAC	2 cut(s)	1091, 2245
HindIII	AAGCTT	3 cut(s)	1364, 1520, 1585
HpaII	CCGG	5 cut(s)	129, 301, 1070, 1179, 1460
HphI	GGTGA	3 cut(s)	34, 286, 331
Hpy166II	GTNNAC	4 cut(s)	36, 604, 1091, 2245
Hpy8I	GTNNAC	4 cut(s)	36, 604, 1091, 2245
Hpy99I	CGWCG	1 cut(s)	469
HpyAV	CCTTC	8 cut(s)	422, 789, 1285, 1606, 1614, 1652, 2162, 2280
HpyCH4III	ACNGT	6 cut(s)	534, 719, 1013, 1023, 1230, 2117
HpyCH4IV	ACGT	6 cut(s)	179, 440, 621, 946, 2201, 2308
HpyF10VI	GCNNNNNNNGC	1 cut(s)	156
HpyF3I	CTNAG	8 cut(s)	83, 516, 612, 925, 1313, 1494, 1860, 2190
HpySE526I	ACGT	6 cut(s)	179, 440, 621, 946, 2201, 2308
Hsp92I	GRCGYC	1 cut(s)	911
HspAI	GCGC	2 cut(s)	628, 1642
Kpn2I	TCCGGA	1 cut(s)	1178
KroI	GCCGGC	1 cut(s)	128
KroNI	GCCGGC	1 cut(s)	130
Kzo9I	GATC	8 cut(s)	321, 595, 1000, 1114, 1294, 1606, 1720, 1969
LmnI	GCTCC	6 cut(s)	164, 362, 638, 1503, 2029, 2171
Lsp1109I	GCAGC	3 cut(s)	642, 948, 2206
LweI	GCATC	3 cut(s)	80, 1738, 1860
MaeI	CTAG	5 cut(s)	146, 587, 1698, 1788, 1988
MaeII	ACGT	6 cut(s)	179, 440, 621, 946, 2201, 2308
MaeIII	GTNAC	2 cut(s)	734, 949
MalI	GATC	8 cut(s)	323, 597, 1002, 1116, 1296, 1608, 1722, 1971
MboI	GATC	8 cut(s)	321, 595, 1000, 1114, 1294, 1606, 1720, 1969
MfeI	CAATTG	1 cut(s)	1033
MflI	RGATCY	2 cut(s)	1000, 1720
MhlI	GDGCHC	1 cut(s)	2082
MlsI	TGGCCA	2 cut(s)	669, 988
MluNI	TGGCCA	2 cut(s)	669, 988
MlyI	GAGTC	5 cut(s)	94, 398, 530, 1923, 1973
MmeI	TCCRAC	4 cut(s)	244, 681, 1816, 2121
Mox20I	TGGCCA	2 cut(s)	669, 988
Mph1103I	ATGCAT	1 cut(s)	73
MreI	CGCCGGCG	1 cut(s)	128
MroI	TCCGGA	1 cut(s)	1178
MroNI	GCCGGC	1 cut(s)	128
MroXI	GAANNNNTTC	1 cut(s)	19
MscI	TGGCCA	2 cut(s)	669, 988
MseI	TTAA	9 cut(s)	980, 1487, 1628, 1703, 2213, 2237, 2258, 2285, 2311
MslI	CAYNNNNRTG	3 cut(s)	956, 1154, 2090
Msp20I	TGGCCA	2 cut(s)	669, 988
MspA1I	CMGCKG	1 cut(s)	1260
MspI	CCGG	5 cut(s)	129, 301, 1070, 1179, 1460
MspR9I	CCNGG	3 cut(s)	375, 483, 547
MunI	CAATTG	1 cut(s)	1033
Mva1269I	GAATGC	3 cut(s)	963, 1536, 2085
MvaI	CCWGG	3 cut(s)	375, 483, 547
MwoI	GCNNNNNNNGC	1 cut(s)	156
NaeI	GCCGGC	1 cut(s)	130
NdeI	CATATG	1 cut(s)	1057
NdeII	GATC	8 cut(s)	321, 595, 1000, 1114, 1294, 1606, 1720, 1969
NgoMIV	GCCGGC	1 cut(s)	128
NlaIV	GGNNCC	1 cut(s)	277
NmeAIII	GCCGAG	1 cut(s)	990
NmuCI	GTSAC	1 cut(s)	949
NsiI	ATGCAT	1 cut(s)	73
NspI	RCATGY	4 cut(s)	778, 1576, 1855, 1997
NspV	TTCGAA	1 cut(s)	2207
PaeI	GCATGC	1 cut(s)	778
PagI	TCATGA	2 cut(s)	822, 1297
PaqCI	CACCTGC	1 cut(s)	53
PciI	ACATGT	1 cut(s)	1851
PcsI	WCGNNNNNNNCGW	2 cut(s)	176, 2213
PctI	GAATGC	3 cut(s)	963, 1536, 2085
PdiI	GCCGGC	1 cut(s)	130
PdmI	GAANNNNTTC	1 cut(s)	19
PfeI	GAWTC	8 cut(s)	189, 257, 397, 1061, 1191, 1592, 2039, 2054
PfoI	TCCNGGA	1 cut(s)	481
PinAI	ACCGGT	1 cut(s)	1459
PkrI	GCNGC	4 cut(s)	632, 963, 2026, 2196
PleI	GAGTC	5 cut(s)	93, 398, 529, 1922, 1972
PmaCI	CACGTG	1 cut(s)	947
PmlI	CACGTG	1 cut(s)	947
PpsI	GAGTC	5 cut(s)	93, 398, 529, 1922, 1972
Ppu21I	YACGTR	3 cut(s)	180, 947, 2202
PscI	ACATGT	1 cut(s)	1851
PshBI	ATTAAT	1 cut(s)	2237
PsiI	TTATAA	1 cut(s)	1395
Psp6I	CCWGG	3 cut(s)	373, 481, 545
PspCI	CACGTG	1 cut(s)	947
PspGI	CCWGG	3 cut(s)	373, 481, 545
PspN4I	GGNNCC	1 cut(s)	277
PspPI	GGNCC	4 cut(s)	575, 1262, 1385, 1457
PsuI	RGATCY	2 cut(s)	1000, 1720
RsaI	GTAC	6 cut(s)	37, 104, 182, 1162, 1571, 1694
RsaNI	GTAC	6 cut(s)	36, 103, 181, 1161, 1570, 1693
RseI	CAYNNNNRTG	3 cut(s)	956, 1154, 2090
SaqAI	TTAA	9 cut(s)	980, 1487, 1628, 1703, 2213, 2237, 2258, 2285, 2311
SatI	GCNGC	4 cut(s)	631, 962, 2025, 2195
Sau3AI	GATC	8 cut(s)	321, 595, 1000, 1114, 1294, 1606, 1720, 1969
Sau96I	GGNCC	4 cut(s)	575, 1262, 1385, 1457
ScaI	AGTACT	1 cut(s)	1694
SchI	GAGTC	5 cut(s)	94, 398, 530, 1923, 1973
ScrFI	CCNGG	3 cut(s)	375, 483, 547
SduI	GDGCHC	1 cut(s)	2082
SfaNI	GCATC	3 cut(s)	80, 1738, 1860
SfcI	CTRYAG	1 cut(s)	715
SfuI	TTCGAA	1 cut(s)	2207
SgrAI	CRCCGGYG	1 cut(s)	128
SinI	GGWCC	3 cut(s)	575, 1262, 1457
SmiMI	CAYNNNNRTG	3 cut(s)	956, 1154, 2090
SmlI	CTYRAG	1 cut(s)	1668
SmoI	CTYRAG	1 cut(s)	1668
SnaBI	TACGTA	1 cut(s)	2202
SphI	GCATGC	1 cut(s)	778
SsiI	CCGC	8 cut(s)	126, 132, 907, 1195, 1260, 1938, 2024, 2027
SspMI	CTAG	5 cut(s)	146, 587, 1698, 1788, 1988
StyD4I	CCNGG	3 cut(s)	373, 481, 545
StyI	CCWWGG	1 cut(s)	117
TaaI	ACNGT	6 cut(s)	534, 719, 1013, 1023, 1230, 2117
TaiI	ACGT	6 cut(s)	182, 443, 624, 949, 2204, 2311
TatI	WGTACW	4 cut(s)	35, 102, 1569, 1692
TauI	GCSGC	1 cut(s)	2027
TfiI	GAWTC	8 cut(s)	189, 257, 397, 1061, 1191, 1592, 2039, 2054
Tru1I	TTAA	9 cut(s)	980, 1487, 1628, 1703, 2213, 2237, 2258, 2285, 2311
Tru9I	TTAA	9 cut(s)	980, 1487, 1628, 1703, 2213, 2237, 2258, 2285, 2311
TscAI	CASTG	3 cut(s)	520, 1204, 1314
TseFI	GTSAC	1 cut(s)	949
TseI	GCWGC	3 cut(s)	630, 961, 2194
Tsp45I	GTSAC	1 cut(s)	949
TspGWI	ACGGA	2 cut(s)	250, 482
TspRI	CASTG	3 cut(s)	520, 1204, 1314
VpaK11BI	GGWCC	3 cut(s)	575, 1262, 1457
VspI	ATTAAT	1 cut(s)	2237
XapI	RAATTY	6 cut(s)	674, 1182, 1277, 1791, 1954, 2209
XbaI	TCTAGA	1 cut(s)	1697
XceI	RCATGY	4 cut(s)	778, 1576, 1855, 1997
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1585
XmnI	GAANNNNTTC	1 cut(s)	19
XspI	CTAG	5 cut(s)	146, 587, 1698, 1788, 1988
ZrmI	AGTACT	1 cut(s)	1694
Zsp2I	ATGCAT	1 cut(s)	73

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.