Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015514

Physical Location & Seq

Genomic Coordinates Reverse (-)

13823903 .. 13826969

Gene Structure 3067 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1176600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2292 bp

ATGAAAACATGGAGACCATTTCATGTTGTGTTCTGTACTTTCGCATGCTTCTTTGTGTGTTTATCTCATGCATCATCGGACACCCTCAGGCATGGACAATACTTGTACAGCAATCAAACCCTGGTTTCTGCCGGCGGGGTGTTTGAGCTAGGCTTTTTCAGCTCCCCCTCAGCGCCGAACGGGTACTTGGGAATCTGGTTCAAGAATGACAAGAACCAAAAGCCAGTTTGGGTTGCAAACAGAAACAGCCCCCTGGATTCCGATGGAATGGTGGGTTCGTCGAGTGTACTTCTCACCATCCGGAATGATGGGAACATGGTGATCATTGACAGAAGAAACATTTCATATATTGTGAATTACGATGCACTTGCGTCAGGGAATAACACAACTGCAACGCTTCTTGACTCAGGAAATCTTGTTCTGATGGAAGGAGGAGACACGTTTTGGCAGAGTTTTGATTACCCGACGGATACGTTTCTTCCTGGAATGAAGTTGGGGTTGTTCAACATAAACACCGAGCAGTTGAGGAAACAGTTTCTTGTGTCCTGGTCAAACACTTCTGTCCCTGCTGATGGTATTTATGCTCTAGGCATTGATCCAGTGAACAACACTCAGTTCAACGTCTGGCGCAGTGATGGAGCTTACCAACAGATTGGACTTTGGGATGGCCATGAGTTTAATTTCTTCTTCAAAAGCACGTTGGATAATCTCAACGTGAGTTCGGGTTGGAAAACTAATGAGATTTATGTTACTTTTAGCAACAAAAATAGTAGCATGCCTTCATGGTTCGTGTTAGCCTCTGAAGGGCTGATTTATGAGTTCACGATGATAGGAAATGAGATCAAGAATGTGGATTATTCACTTTGTGATGACACAATGGCAAGCAGTTCTCCTCGCTGTTTGGTGAGGAGGCCATCAGCGTGTTCAGAAAGAGATAGTTTCTCAAATGTCACAGGAATGCTGCCGAGTTCATTGTTTACAAGTTGGCCAATTCGGATAGATCCCTATGACTGCGAGAAATTTTGCACGATCCATTGCTCATGTGTTGCATATACATATGATTCTTTGCCGGATGGTGGAACTATATGTCAGGTTTATTATGGAAATAAAATCGATCTTCAAAAACTGGTCGGGACAGGTAACAGCACCATTTATGTGCGCGGTAACGCTTCTAAATCTGGAATTTATGGATTCCGCAATGGCACGAGAGATAGTATGAGGCTATTATTGTCGCAGTTGAGCTCCGATGATAATGCTAGCTGTCCAAATATTACAGAACTTGGAAAGCAGAAGGATCATGAGTTGCCACTGCTTAGCTTTTCATTCATAGTGACATCCACTAATAATTTCTCCCTTGCAAATCAGCTTGGACAAGGTGGTTTCGGGCCTGTTTATAAGGGAAAATTACTACAACATGACATTGCAGTCAAAAGGCTATCCAAAAATTCTGGACAAGGACCCGTGGAGTTCAAGAACGAGGTGCAGTTAATCTCTAAGCTCCAGCACAGAAACCTTGTCAAGCTTTTGGGCTTTTGCATTCACCGAGAAGAAAAGATACTAATATACGAGTACATGCCCAATAAAAGCTTGGATTCCTTCATTTTCGAAAAAACAAAGACAAGGTCATTGAATTGGAGACAGCGCATACACATAATCAAAGGGATTGCTCAAGGGCTTCTTTATCTTCACAAGTACTCTAGATTAAGAATTATTCATCGCGATCTTAAAATTAGCAACATCTTATTGGATGCTTACATGAACCCCAAGATATCAGACTTTGGCTTGGCTAGAATTTTATCCGGGAATGAATGTCGAGCCAAAACAAATCGGGTTGTTGGAACATATGGTTATATGTCTCCCGAGTATCTGATGCACGGCCTTTTCTCAACTAAATCCGATGTGTTCAGCTTCGGAGTCATTGTGTTGGAGATTGTAAGTGGCAGGAAGAACGCAAACTTTTATGAGTCAGATCATTCTCTGAACCTACTAGGACATGCTTGGAATTTATGGAAATCTGGGCGAGCAGCGGAGTTGATGGATTCAACACTGGCTGATTCAAGTTCAATGGAAAGCCTTGTAACCTGCATTCAGGTGGGTCTGTTATGTGTCCAAGACAGTGCAGAGGATAGGCCAACCATGTCGGATGTTGTTTCAATGTTGAGCAATGAAGGGGCAACTTTACCTACCCCTAAGCAGACTACGTATTCGAATTTAACGACAAAGGTTGATAAACCATTAAATCAATTGCCTTCTGTAAATCTATTAACATTTTCGGAAGTAGAAGCACGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase_Tyr

Protein Analysis

764

Amino Acids

85.37

Weight (kDa)

7.51

Isoelectric Point (pI)

41.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	76 - 183	3e-25	D-mannose binding lectin
PAN_2	PF08276	308 - 372	9.2e-07	PAN-like domain
PK_Tyr_Ser-Thr	PF07714	452 - 720	2.2e-48	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	452 - 717	1.7e-44	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015514)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g03780
malus_domestica	MD02G1036400.v1.1 MD15G1176600.v1.1
prunus_persica	Prupe.7G239000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0089191
rosa_laevigata	RLG00000016003
rosa_multiflora	Rmu_sc0008322.1_g000027 Rmu_sc0008909.1_g000009
rosa_roxburghii	Rroxscaffold_2G00152160
rosa_rugosa	Rorug01G0488300
rosa_samantha	Rh2AG043900 Rh2BG042600 Rh2CG044100 Rh2DG042900
rosa_wichuraiana	Rw2G003920 Rw2G003930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1395
AasI	GACNNNNNNGTC	1 cut(s)	1424
Acc36I	ACCTGC	1 cut(s)	2090
AccII	CGCG	2 cut(s)	1161, 1719
AccIII	TCCGGA	1 cut(s)	300
AciI	CCGC	4 cut(s)	135, 1161, 1195, 2027
AclWI	GGATC	4 cut(s)	590, 995, 1024, 1302
AcoI	YGGCCR	2 cut(s)	667, 986
AcsI	RAATTY	6 cut(s)	1019, 1182, 1444, 1791, 2002, 2209
AcuI	CTGAAG	1 cut(s)	822
AdeI	CACNNNGTG	1 cut(s)	866
AfaI	GTAC	6 cut(s)	37, 107, 185, 288, 1571, 1694
AfiI	CCNNNNNNNGG	3 cut(s)	572, 804, 1076
AflIII	ACRYGT	1 cut(s)	438
AjnI	CCWGG	4 cut(s)	120, 252, 481, 545
AjuI	GAANNNNNNNTTGG	2 cut(s)	170, 202
AloI	GAACNNNNNNTCC	2 cut(s)	402, 434
Alw21I	GWGCWC	1 cut(s)	1244
Alw26I	GTCTC	4 cut(s)	7, 429, 1630, 1860
AlwI	GGATC	4 cut(s)	590, 995, 1024, 1302
Ama87I	CYCGRG	1 cut(s)	1859
Aor13HI	TCCGGA	1 cut(s)	300
AoxI	GGCC	6 cut(s)	667, 911, 986, 1385, 1876, 2129
ApeKI	GCWGC	2 cut(s)	961, 2024
ApoI	RAATTY	6 cut(s)	1019, 1182, 1444, 1791, 2002, 2209
ArsI	GACNNNNNNTTYG	4 cut(s)	1607, 1639, 2188, 2220
Asp700I	GAANNNNTTC	1 cut(s)	340
AspLEI	GCGC	4 cut(s)	175, 630, 1161, 1644
AspS9I	GGNCC	2 cut(s)	1385, 1457
AsuC2I	CCSGG	1 cut(s)	1801
AsuHPI	GGTGA	4 cut(s)	286, 331, 916, 1532
AsuII	TTCGAA	2 cut(s)	1605, 2207
AsuNHI	GCTAGC	1 cut(s)	1256
AvaI	CYCGRG	1 cut(s)	1859
AvaII	GGWCC	1 cut(s)	1457
AxyI	CCTNAGG	1 cut(s)	86
BalI	TGGCCA	2 cut(s)	669, 988
BanII	GRGCYC	1 cut(s)	1244
BarI	GAAGNNNNNNTAC	4 cut(s)	763, 795, 1539, 1571
BauI	CACGAG	1 cut(s)	1204
Bbv12I	GWGCWC	1 cut(s)	1244
BbvCI	CCTCAGC	1 cut(s)	169
BbvI	GCAGC	2 cut(s)	948, 2036
BceAI	ACGGC	1 cut(s)	1891
BcgI	CGANNNNNNTGC	4 cut(s)	350, 384, 1235, 1269
BciT130I	CCWGG	4 cut(s)	122, 254, 483, 547
BciVI	GTATCC	1 cut(s)	463
BclI	TGATCA	1 cut(s)	321
BcnI	CCSGG	1 cut(s)	1801
BcoDI	GTCTC	4 cut(s)	7, 429, 1630, 1860
BfaI	CTAG	6 cut(s)	149, 587, 1257, 1698, 1788, 1988
BfoI	RGCGCY	1 cut(s)	176
BfuAI	ACCTGC	1 cut(s)	2090
BfuI	GTATCC	1 cut(s)	463
BisI	GCNGC	2 cut(s)	962, 2025
BlpI	GCTNAGC	1 cut(s)	1313
BlsI	GCNGC	2 cut(s)	963, 2026
BmcAI	AGTACT	1 cut(s)	1694
Bme1390I	CCNGG	5 cut(s)	122, 254, 483, 547, 1801
Bme18I	GGWCC	1 cut(s)	1457
BmeT110I	CYCGRG	1 cut(s)	1859
BmgT120I	GGNCC	2 cut(s)	1385, 1457
BmiI	GGNNCC	1 cut(s)	1459
BmrFI	CCNGG	5 cut(s)	122, 254, 483, 547, 1801
BmsI	GCATC	4 cut(s)	80, 352, 1738, 1860
BmtI	GCTAGC	1 cut(s)	1260
BpmI	CTGGAG	1 cut(s)	1484
Bpu10I	CCTNAGC	2 cut(s)	169, 2190
Bpu1102I	GCTNAGC	1 cut(s)	1313
Bpu14I	TTCGAA	2 cut(s)	1605, 2207
BpuEI	CTTGAG	1 cut(s)	1653
BpuMI	CCSGG	1 cut(s)	1801
Bsa29I	ATCGAT	1 cut(s)	1113
BsaAI	YACGTR	1 cut(s)	2202
BsaI	GGTCTC	1 cut(s)	7
BsaJI	CCNNGG	3 cut(s)	120, 252, 1461
BsaWI	WCCGGW	1 cut(s)	300
BsaXI	ACNNNNNCTCC	2 cut(s)	1905, 1935
Bsc4I	CCNNNNNNNGG	3 cut(s)	572, 804, 1076
Bse118I	RCCGGY	1 cut(s)	131
Bse1I	ACTGG	4 cut(s)	224, 599, 1131, 2052
Bse21I	CCTNAGG	1 cut(s)	86
Bse3DI	GCAATG	4 cut(s)	1033, 1204, 1419, 2170
BseAI	TCCGGA	1 cut(s)	300
BseBI	CCWGG	4 cut(s)	122, 254, 483, 547
BseCI	ATCGAT	1 cut(s)	1113
BseDI	CCNNGG	3 cut(s)	120, 252, 1461
BseGI	GGATG	6 cut(s)	297, 670, 1078, 1334, 1753, 2149
BseLI	CCNNNNNNNGG	3 cut(s)	572, 804, 1076
BseMI	GCAATG	4 cut(s)	1033, 1204, 1419, 2170
BseMII	CTCAG	4 cut(s)	100, 183, 420, 626
BseNI	ACTGG	4 cut(s)	224, 599, 1131, 2052
BseRI	GAGGAG	3 cut(s)	447, 882, 922
BseXI	GCAGC	2 cut(s)	948, 2036
BsgI	GTGCAG	2 cut(s)	1502, 2139
Bsh1236I	CGCG	2 cut(s)	1161, 1719
BshFI	GGCC	6 cut(s)	669, 913, 988, 1387, 1878, 2131
BshVI	ATCGAT	1 cut(s)	1113
BsiHKAI	GWGCWC	1 cut(s)	1244
BsiHKCI	CYCGRG	1 cut(s)	1859
BsiSI	CCGG	4 cut(s)	132, 301, 1070, 1800
BslFI	GGGAC	2 cut(s)	548, 1147
BslI	CCNNNNNNNGG	3 cut(s)	572, 804, 1076
BsmAI	GTCTC	4 cut(s)	7, 429, 1630, 1860
BsmFI	GGGAC	2 cut(s)	548, 1147
BsmI	GAATGC	3 cut(s)	963, 1536, 2085
BsnI	GGCC	6 cut(s)	669, 913, 988, 1387, 1878, 2131
Bso31I	GGTCTC	1 cut(s)	7
BsoBI	CYCGRG	1 cut(s)	1859
Bsp119I	TTCGAA	2 cut(s)	1605, 2207
Bsp1286I	GDGCHC	1 cut(s)	1244
Bsp13I	TCCGGA	1 cut(s)	300
Bsp1407I	TGTACA	1 cut(s)	105
Bsp143I	GATC	9 cut(s)	321, 595, 840, 1000, 1029, 1114, 1294, 1720, 1969
Bsp1720I	GCTNAGC	1 cut(s)	1313
Bsp68I	TCGCGA	1 cut(s)	1719
BspACI	CCGC	4 cut(s)	135, 1161, 1195, 2027
BspANI	GGCC	6 cut(s)	669, 913, 988, 1387, 1878, 2131
BspCNI	CTCAG	4 cut(s)	99, 182, 419, 625
BspDI	ATCGAT	1 cut(s)	1113
BspEI	TCCGGA	1 cut(s)	300
BspFNI	CGCG	2 cut(s)	1161, 1719
BspHI	TCATGA	1 cut(s)	1297
BspLI	GGNNCC	1 cut(s)	1459
BspMI	ACCTGC	1 cut(s)	2090
BspOI	GCTAGC	1 cut(s)	1260
BspPI	GGATC	4 cut(s)	590, 995, 1024, 1302
BspT104I	TTCGAA	2 cut(s)	1605, 2207
BspTNI	GGTCTC	1 cut(s)	7
BsrDI	GCAATG	4 cut(s)	1033, 1204, 1419, 2170
BsrFI	RCCGGY	1 cut(s)	131
BsrGI	TGTACA	1 cut(s)	105
BsrI	ACTGG	4 cut(s)	224, 599, 1131, 2052
BssAI	RCCGGY	1 cut(s)	131
BssECI	CCNNGG	3 cut(s)	120, 252, 1461
BssMI	GATC	9 cut(s)	321, 595, 840, 1000, 1029, 1114, 1294, 1720, 1969
BssSI	CACGAG	1 cut(s)	1204
Bst2BI	CACGAG	1 cut(s)	1204
Bst2UI	CCWGG	4 cut(s)	122, 254, 483, 547
Bst4CI	ACNGT	2 cut(s)	534, 2117
BstAUI	TGTACA	1 cut(s)	105
BstBAI	YACGTR	1 cut(s)	2202
BstBI	TTCGAA	2 cut(s)	1605, 2207
BstC8I	GCNNGC	6 cut(s)	46, 133, 776, 883, 1258, 2022
BstDEI	CTNAG	7 cut(s)	86, 169, 406, 612, 1313, 1494, 2190
BstDSI	CCRYGG	1 cut(s)	1461
BstF5I	GGATG	6 cut(s)	297, 670, 1078, 1334, 1753, 2149
BstFNI	CGCG	2 cut(s)	1161, 1719
BstH2I	RGCGCY	1 cut(s)	176
BstHHI	GCGC	4 cut(s)	175, 630, 1161, 1644
BstKTI	GATC	9 cut(s)	324, 598, 843, 1003, 1032, 1117, 1297, 1723, 1972
BstMAI	GTCTC	4 cut(s)	7, 429, 1630, 1860
BstMBI	GATC	9 cut(s)	321, 595, 840, 1000, 1029, 1114, 1294, 1720, 1969
BstMWI	GCNNNNNNNGC	1 cut(s)	159
BstNI	CCWGG	4 cut(s)	122, 254, 483, 547
BstNSI	RCATGY	4 cut(s)	48, 778, 1576, 1997
BstSCI	CCNGG	5 cut(s)	120, 252, 481, 545, 1799
BstSNI	TACGTA	1 cut(s)	2202
BstUI	CGCG	2 cut(s)	1161, 1719
BstV1I	GCAGC	2 cut(s)	948, 2036
BstX2I	RGATCY	1 cut(s)	1000
BstXI	CCANNNNNNTGG	1 cut(s)	653
BstYI	RGATCY	1 cut(s)	1000
Bsu15I	ATCGAT	1 cut(s)	1113
Bsu36I	CCTNAGG	1 cut(s)	86
BsuI	GTATCC	1 cut(s)	463
BsuRI	GGCC	6 cut(s)	669, 913, 988, 1387, 1878, 2131
BsuTUI	ATCGAT	1 cut(s)	1113
BtgI	CCRYGG	1 cut(s)	1461
BtgZI	GCGATG	1 cut(s)	1700
BtsCI	GGATG	6 cut(s)	297, 670, 1078, 1334, 1753, 2149
BtsI	GCAGTG	2 cut(s)	637, 1307
BtsIMutI	CAGTG	5 cut(s)	606, 637, 1307, 2045, 2122
BtuMI	TCGCGA	1 cut(s)	1719
BveI	ACCTGC	1 cut(s)	2090
Cac8I	GCNNGC	6 cut(s)	46, 133, 776, 883, 1258, 2022
CciI	TCATGA	1 cut(s)	1297
CfoI	GCGC	4 cut(s)	175, 630, 1161, 1644
Cfr10I	RCCGGY	1 cut(s)	131
Cfr13I	GGNCC	2 cut(s)	1385, 1457
ClaI	ATCGAT	1 cut(s)	1113
CseI	GACGC	1 cut(s)	360
Csp6I	GTAC	6 cut(s)	36, 106, 184, 287, 1570, 1693
CviQI	GTAC	6 cut(s)	36, 106, 184, 287, 1570, 1693
DdeI	CTNAG	7 cut(s)	86, 169, 406, 612, 1313, 1494, 2190
DpnI	GATC	9 cut(s)	323, 597, 842, 1002, 1031, 1116, 1296, 1722, 1971
DpnII	GATC	9 cut(s)	321, 595, 840, 1000, 1029, 1114, 1294, 1720, 1969
DraIII	CACNNNGTG	1 cut(s)	866
DrdI	GACNNNNNNGTC	1 cut(s)	1424
DseDI	GACNNNNNNGTC	1 cut(s)	1424
EaeI	YGGCCR	2 cut(s)	667, 986
Ecl136II	GAGCTC	1 cut(s)	1242
Eco105I	TACGTA	1 cut(s)	2202
Eco24I	GRGCYC	1 cut(s)	1244
Eco31I	GGTCTC	1 cut(s)	7
Eco32I	GATATC	1 cut(s)	1770
Eco47I	GGWCC	1 cut(s)	1457
Eco53kI	GAGCTC	1 cut(s)	1242
Eco57I	CTGAAG	1 cut(s)	822
Eco81I	CCTNAGG	1 cut(s)	86
Eco88I	CYCGRG	1 cut(s)	1859
EcoICRI	GAGCTC	1 cut(s)	1242
EcoO109I	RGGNCCY	1 cut(s)	1457
EcoRII	CCWGG	4 cut(s)	120, 252, 481, 545
EcoRV	GATATC	1 cut(s)	1770
EcoT22I	ATGCAT	1 cut(s)	73
EcoT38I	GRGCYC	1 cut(s)	1244
FalI	AAGNNNNNCTT	2 cut(s)	1662, 1694
FaqI	GGGAC	2 cut(s)	548, 1147
FauI	CCCGC	1 cut(s)	128
FauNDI	CATATG	2 cut(s)	1057, 1843
FbaI	TGATCA	1 cut(s)	321
Fnu4HI	GCNGC	2 cut(s)	962, 2025
FokI	GGATG	6 cut(s)	284, 677, 1085, 1321, 1760, 2156
FriOI	GRGCYC	1 cut(s)	1244
Fsp4HI	GCNGC	2 cut(s)	962, 2025
FspBI	CTAG	6 cut(s)	149, 587, 1257, 1698, 1788, 1988
GlaI	GCGC	4 cut(s)	174, 629, 1160, 1643
GluI	GCNGC	2 cut(s)	962, 2025
GsuI	CTGGAG	1 cut(s)	1484
HaeII	RGCGCY	1 cut(s)	176
HaeIII	GGCC	6 cut(s)	669, 913, 988, 1387, 1878, 2131
HapII	CCGG	4 cut(s)	132, 301, 1070, 1800
HgaI	GACGC	1 cut(s)	360
HhaI	GCGC	4 cut(s)	175, 630, 1161, 1644
Hin6I	GCGC	4 cut(s)	173, 628, 1159, 1642
HinP1I	GCGC	4 cut(s)	173, 628, 1159, 1642
HindIII	AAGCTT	2 cut(s)	1520, 1585
HpaII	CCGG	4 cut(s)	132, 301, 1070, 1800
HphI	GGTGA	4 cut(s)	286, 331, 916, 1532
Hpy166II	GTNNAC	4 cut(s)	287, 604, 822, 978
Hpy8I	GTNNAC	4 cut(s)	287, 604, 822, 978
Hpy99I	CGWCG	2 cut(s)	283, 469
HpyAV	CCTTC	7 cut(s)	422, 789, 797, 1285, 1606, 2162, 2259
HpyCH4III	ACNGT	2 cut(s)	534, 2117
HpyCH4IV	ACGT	6 cut(s)	440, 473, 621, 698, 714, 2201
HpyF10VI	GCNNNNNNNGC	1 cut(s)	159
HpyF3I	CTNAG	7 cut(s)	86, 169, 406, 612, 1313, 1494, 2190
HpySE526I	ACGT	6 cut(s)	440, 473, 621, 698, 714, 2201
HspAI	GCGC	4 cut(s)	173, 628, 1159, 1642
Kpn2I	TCCGGA	1 cut(s)	300
KroI	GCCGGC	1 cut(s)	131
KroNI	GCCGGC	1 cut(s)	133
Ksp22I	TGATCA	1 cut(s)	321
Kzo9I	GATC	9 cut(s)	321, 595, 840, 1000, 1029, 1114, 1294, 1720, 1969
LmnI	GCTCC	4 cut(s)	167, 638, 1247, 1503
Lsp1109I	GCAGC	2 cut(s)	948, 2036
LweI	GCATC	4 cut(s)	80, 352, 1738, 1860
MaeI	CTAG	6 cut(s)	149, 587, 1257, 1698, 1788, 1988
MaeII	ACGT	6 cut(s)	440, 473, 621, 698, 714, 2201
MaeIII	GTNAC	6 cut(s)	748, 949, 1139, 1163, 1330, 2077
MalI	GATC	9 cut(s)	323, 597, 842, 1002, 1031, 1116, 1296, 1722, 1971
MboI	GATC	9 cut(s)	321, 595, 840, 1000, 1029, 1114, 1294, 1720, 1969
MboII	GAAGA	8 cut(s)	345, 470, 676, 679, 1109, 1559, 1676, 1957
MfeI	CAATTG	1 cut(s)	2243
MflI	RGATCY	1 cut(s)	1000
MhlI	GDGCHC	1 cut(s)	1244
MlsI	TGGCCA	2 cut(s)	669, 988
MluNI	TGGCCA	2 cut(s)	669, 988
MlyI	GAGTC	3 cut(s)	398, 1923, 1973
MmeI	TCCRAC	5 cut(s)	681, 707, 1816, 1905, 2121
Mox20I	TGGCCA	2 cut(s)	669, 988
Mph1103I	ATGCAT	1 cut(s)	73
MroI	TCCGGA	1 cut(s)	300
MroNI	GCCGGC	1 cut(s)	131
MroXI	GAANNNNTTC	1 cut(s)	340
MscI	TGGCCA	2 cut(s)	669, 988
MseI	TTAA	7 cut(s)	678, 1487, 1703, 1725, 2213, 2237, 2264
MslI	CAYNNNNRTG	4 cut(s)	919, 956, 1154, 2090
Msp20I	TGGCCA	2 cut(s)	669, 988
MspA1I	CMGCKG	1 cut(s)	2027
MspI	CCGG	4 cut(s)	132, 301, 1070, 1800
MspR9I	CCNGG	5 cut(s)	122, 254, 483, 547, 1801
MunI	CAATTG	1 cut(s)	2243
Mva1269I	GAATGC	3 cut(s)	963, 1536, 2085
MvaI	CCWGG	4 cut(s)	122, 254, 483, 547
MvnI	CGCG	2 cut(s)	1161, 1719
MwoI	GCNNNNNNNGC	1 cut(s)	159
NaeI	GCCGGC	1 cut(s)	133
NciI	CCSGG	1 cut(s)	1801
NdeI	CATATG	2 cut(s)	1057, 1843
NdeII	GATC	9 cut(s)	321, 595, 840, 1000, 1029, 1114, 1294, 1720, 1969
NgoMIV	GCCGGC	1 cut(s)	131
NheI	GCTAGC	1 cut(s)	1256
NlaIV	GGNNCC	1 cut(s)	1459
NmeAIII	GCCGAG	1 cut(s)	990
NmuCI	GTSAC	2 cut(s)	949, 1330
NruI	TCGCGA	1 cut(s)	1719
NsiI	ATGCAT	1 cut(s)	73
NspI	RCATGY	4 cut(s)	48, 778, 1576, 1997
NspV	TTCGAA	2 cut(s)	1605, 2207
PaeI	GCATGC	2 cut(s)	48, 778
PagI	TCATGA	1 cut(s)	1297
PcsI	WCGNNNNNNNCGW	1 cut(s)	2213
PctI	GAATGC	3 cut(s)	963, 1536, 2085
PdiI	GCCGGC	1 cut(s)	133
PdmI	GAANNNNTTC	1 cut(s)	340
PfeI	GAWTC	7 cut(s)	192, 257, 1061, 1191, 1592, 2039, 2054
PflFI	GACNNNGTC	1 cut(s)	1621
PfoI	TCCNGGA	2 cut(s)	481, 1799
PkrI	GCNGC	2 cut(s)	963, 2026
PleI	GAGTC	3 cut(s)	398, 1922, 1972
PpsI	GAGTC	3 cut(s)	398, 1922, 1972
Ppu21I	YACGTR	1 cut(s)	2202
PpuMI	RGGWCCY	1 cut(s)	1457
PsiI	TTATAA	1 cut(s)	1395
Psp124BI	GAGCTC	1 cut(s)	1244
Psp5II	RGGWCCY	1 cut(s)	1457
Psp6I	CCWGG	4 cut(s)	120, 252, 481, 545
PspGI	CCWGG	4 cut(s)	120, 252, 481, 545
PspN4I	GGNNCC	1 cut(s)	1459
PspPI	GGNCC	2 cut(s)	1385, 1457
PspPPI	RGGWCCY	1 cut(s)	1457
PsuI	RGATCY	1 cut(s)	1000
PsyI	GACNNNGTC	1 cut(s)	1621
RruI	TCGCGA	1 cut(s)	1719
RsaI	GTAC	6 cut(s)	37, 107, 185, 288, 1571, 1694
RsaNI	GTAC	6 cut(s)	36, 106, 184, 287, 1570, 1693
RseI	CAYNNNNRTG	4 cut(s)	919, 956, 1154, 2090
SacI	GAGCTC	1 cut(s)	1244
SaqAI	TTAA	7 cut(s)	678, 1487, 1703, 1725, 2213, 2237, 2264
SatI	GCNGC	2 cut(s)	962, 2025
Sau3AI	GATC	9 cut(s)	321, 595, 840, 1000, 1029, 1114, 1294, 1720, 1969
Sau96I	GGNCC	2 cut(s)	1385, 1457
ScaI	AGTACT	1 cut(s)	1694
SchI	GAGTC	3 cut(s)	398, 1923, 1973
ScrFI	CCNGG	5 cut(s)	122, 254, 483, 547, 1801
SduI	GDGCHC	1 cut(s)	1244
SfaNI	GCATC	4 cut(s)	80, 352, 1738, 1860
SfuI	TTCGAA	2 cut(s)	1605, 2207
SinI	GGWCC	1 cut(s)	1457
SmiMI	CAYNNNNRTG	4 cut(s)	919, 956, 1154, 2090
SmlI	CTYRAG	1 cut(s)	1668
SmoI	CTYRAG	1 cut(s)	1668
SnaBI	TACGTA	1 cut(s)	2202
SphI	GCATGC	2 cut(s)	48, 778
SsiI	CCGC	4 cut(s)	135, 1161, 1195, 2027
SspI	AATATT	1 cut(s)	1270
SspMI	CTAG	6 cut(s)	149, 587, 1257, 1698, 1788, 1988
SstI	GAGCTC	1 cut(s)	1244
StyD4I	CCNGG	5 cut(s)	120, 252, 481, 545, 1799
TaaI	ACNGT	2 cut(s)	534, 2117
TaiI	ACGT	6 cut(s)	443, 476, 624, 701, 717, 2204
TaqI	TCGA	5 cut(s)	281, 1113, 1605, 1813, 2207
TatI	WGTACW	5 cut(s)	35, 105, 286, 1569, 1692
TfiI	GAWTC	7 cut(s)	192, 257, 1061, 1191, 1592, 2039, 2054
Tru1I	TTAA	7 cut(s)	678, 1487, 1703, 1725, 2213, 2237, 2264
Tru9I	TTAA	7 cut(s)	678, 1487, 1703, 1725, 2213, 2237, 2264
TscAI	CASTG	5 cut(s)	606, 637, 1314, 2052, 2122
TseFI	GTSAC	2 cut(s)	949, 1330
TseI	GCWGC	2 cut(s)	961, 2024
Tsp45I	GTSAC	2 cut(s)	949, 1330
TspGWI	ACGGA	1 cut(s)	482
TspRI	CASTG	5 cut(s)	606, 637, 1314, 2052, 2122
Tth111I	GACNNNGTC	1 cut(s)	1621
VpaK11BI	GGWCC	1 cut(s)	1457
XapI	RAATTY	6 cut(s)	1019, 1182, 1444, 1791, 2002, 2209
XbaI	TCTAGA	1 cut(s)	1697
XceI	RCATGY	4 cut(s)	48, 778, 1576, 1997
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1585
XmnI	GAANNNNTTC	1 cut(s)	340
XspI	CTAG	6 cut(s)	149, 587, 1257, 1698, 1788, 1988
ZrmI	AGTACT	1 cut(s)	1694
Zsp2I	ATGCAT	1 cut(s)	73

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.