Rw2G003920

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015514
Physical Location & Seq
Forward (+)
3686671 .. 3687699
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003920.1

Sequence Viewer

Length: 1029 bp
ATGAAAATATTGAGAGCTGCTTCTGTTTTCCTTTGTATTTTCTTCTGCTTCTTTGTTTGCTCATCTCATGCAACAGATACCCTTAGGCCTGGTCAGTATTTGACGAGATATGAAACCTTGGTTTCTGATGGTGGAGTATTTGAGCTAGGCTTTTTCTACTGCCCCACTAAGTCAGTCGAAGGATACTTGGGAATCTGGTTGAAGAATGACAAAAAGAAAAGGCCAGTTTGGGTGGCAAATAGAGATGACCATCTTATGGATCCCTATGCACTTCTCTACATCAACCCTCAGGGAATGGTGATCAGTGATAATAGAAATTCACCGTTTTTTTTCACTTATGGAGCAACTGCAACGACTAATAGGACAAGAGCAAGGATTCTTGACTCAGGAAACCTAATCCTGATGGAAGGAGGAAAGACGGTGTGGCAGAGCTTTGATAACCTGGTAGATACATTTCTTCCTGGAATGAAAATAGGGATTGTTAGCACAACCAATGCTCAGTTGATGCCTATGTTTCTTTTATCATGGAGAAGCGCACATGACCCTACTCGTGGTTTTCTTGGTTTAAGCTTGGATAACAGCACAACTCAAGTCGATGTCTGGCACGATGATGGTAAAGAGAAACCAATTGGCCATTGGGATGGCCATGAGTTCAAATTTGTTTTCAAAACCCAATCAGATAATTACAATTTCAGCTATGTACTGAACAGCAAGGAGCGTTATCTTACTTTCAACAACAAAGAAAGTAGTGTATATTCATGGTTTGCATTGGCTTCGGATGGTAAGATACAGGAGTTCATGATGGTAGGACAGAAGATTTCGGTTGTGAGTCATTCGGTATGTGGTGACTGCTGGTTGGTGTCATCAAGAGATAGGAAGCTGTTGCTGGTCATCAAAGTTGTGGTTCCCTTGACTTTCTCCATTTTGATGTTGATCTTGTTCCTGTATTGTTTATGGAGAAAGCACAACTCTTTAGGTACGTTATCCAAACTGTATGAACTGAAATATGGTAAACTTTGCCAAATATAA

Protein Analysis

342

Amino Acids

39.18

Weight (kDa)

8.88

Isoelectric Point (pI)

24.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 177 2.9e-21 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015514)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03780
malus_domestica MD02G1036400.v1.1 MD15G1176600.v1.1
prunus_persica Prupe.7G239000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0089191
rosa_laevigata RLG00000016003
rosa_multiflora Rmu_sc0008322.1_g000027 Rmu_sc0008909.1_g000009
rosa_roxburghii Rroxscaffold_2G00152160
rosa_rugosa Rorug01G0488300
rosa_samantha Rh2AG043900 Rh2BG042600 Rh2CG044100 Rh2DG042900
rosa_wichuraiana Rw2G003920 Rw2G003930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 256
AclWI GGATC 2 cut(s) 254, 267
AcoI YGGCCR 2 cut(s) 631, 643
AcsI RAATTY 2 cut(s) 316, 656
AfaI GTAC 2 cut(s) 702, 979
AfiI CCNNNNNNNGG 2 cut(s) 256, 551
AgsI TTSAA 4 cut(s) 202, 655, 667, 733
AjnI CCWGG 3 cut(s) 88, 441, 460
AluBI AGCT 6 cut(s) 17, 145, 432, 570, 696, 880
AluI AGCT 6 cut(s) 17, 145, 432, 570, 696, 880
AlwI GGATC 2 cut(s) 254, 267
AoxI GGCC 4 cut(s) 86, 221, 631, 643
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 2 cut(s) 316, 656
AspLEI GCGC 1 cut(s) 536
AsuHPI GGTGA 3 cut(s) 310, 312, 857
AxyI CCTNAGG 2 cut(s) 83, 288
BaeI ACNNNNGTAYC 2 cut(s) 779, 812
BalI TGGCCA 2 cut(s) 633, 645
BamHI GGATCC 1 cut(s) 259
BauI CACGAG 1 cut(s) 549
BbvI GCAGC 1 cut(s) 4
BccI CCATC 7 cut(s) 122, 258, 397, 605, 635, 773, 796
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciT130I CCWGG 3 cut(s) 90, 443, 462
BciVI GTATCC 1 cut(s) 176
BclI TGATCA 1 cut(s) 300
BfaI CTAG 1 cut(s) 146
BfuI GTATCC 1 cut(s) 176
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 3 cut(s) 90, 443, 462
BmiI GGNNCC 2 cut(s) 261, 906
BmrFI CCNGG 3 cut(s) 90, 443, 462
BmsI GCATC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 573
BsaBI GATNNNNATC 2 cut(s) 249, 932
BsaJI CCNNGG 1 cut(s) 117
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 551
Bse1I ACTGG 1 cut(s) 224
Bse21I CCTNAGG 2 cut(s) 83, 288
Bse8I GATNNNNATC 2 cut(s) 249, 932
BseBI CCWGG 3 cut(s) 90, 443, 462
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 2 cut(s) 646, 784
BseJI GATNNNNATC 2 cut(s) 249, 932
BseLI CCNNNNNNNGG 2 cut(s) 256, 551
BseMII CTCAG 3 cut(s) 302, 399, 512
BseNI ACTGG 1 cut(s) 224
BseXI GCAGC 1 cut(s) 4
BshFI GGCC 4 cut(s) 88, 223, 633, 645
BslI CCNNNNNNNGG 2 cut(s) 256, 551
BsnI GGCC 4 cut(s) 88, 223, 633, 645
Bsp143I GATC 3 cut(s) 259, 300, 933
BspANI GGCC 4 cut(s) 88, 223, 633, 645
BspCNI CTCAG 3 cut(s) 301, 398, 511
BspHI TCATGA 1 cut(s) 798
BspLI GGNNCC 2 cut(s) 261, 906
BspPI GGATC 2 cut(s) 254, 267
BsrI ACTGG 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 3 cut(s) 259, 300, 933
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 117
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 3 cut(s) 90, 443, 462
Bst4CI ACNGT 3 cut(s) 324, 421, 993
BstDEI CTNAG 5 cut(s) 83, 168, 288, 385, 498
BstF5I GGATG 2 cut(s) 646, 784
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 3 cut(s) 262, 303, 936
BstMBI GATC 3 cut(s) 259, 300, 933
BstNI CCWGG 3 cut(s) 90, 443, 462
BstSCI CCNGG 3 cut(s) 88, 441, 460
BstV1I GCAGC 1 cut(s) 4
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 1 cut(s) 641
BstYI RGATCY 1 cut(s) 259
Bsu36I CCTNAGG 2 cut(s) 83, 288
BsuI GTATCC 1 cut(s) 176
BsuRI GGCC 4 cut(s) 88, 223, 633, 645
BtsCI GGATG 2 cut(s) 646, 784
BtsIMutI CAGTG 1 cut(s) 310
CciI TCATGA 1 cut(s) 798
CfoI GCGC 1 cut(s) 536
CsiI ACCWGGT 1 cut(s) 441
Csp6I GTAC 2 cut(s) 701, 978
CviAII CATG 6 cut(s) 68, 525, 539, 647, 759, 799
CviQI GTAC 2 cut(s) 701, 978
DdeI CTNAG 5 cut(s) 83, 168, 288, 385, 498
DpnI GATC 3 cut(s) 261, 302, 935
DpnII GATC 3 cut(s) 259, 300, 933
EaeI YGGCCR 2 cut(s) 631, 643
Eco130I CCWWGG 1 cut(s) 117
Eco147I AGGCCT 1 cut(s) 88
Eco81I CCTNAGG 2 cut(s) 83, 288
EcoRII CCWGG 3 cut(s) 88, 441, 460
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 6 cut(s) 71, 528, 542, 650, 762, 802
FatI CATG 6 cut(s) 67, 524, 538, 646, 758, 798
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 2 cut(s) 653, 791
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 1 cut(s) 146
GlaI GCGC 1 cut(s) 535
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 4 cut(s) 88, 223, 633, 645
HhaI GCGC 1 cut(s) 536
Hin1II CATG 6 cut(s) 71, 528, 542, 650, 762, 802
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HindIII AAGCTT 1 cut(s) 568
HinfI GANTC 4 cut(s) 192, 376, 383, 829
HphI GGTGA 3 cut(s) 310, 312, 857
Hpy166II GTNNAC 1 cut(s) 1013
Hpy188I TCNGA 3 cut(s) 127, 679, 778
Hpy188III TCNNGA 5 cut(s) 380, 387, 400, 799, 867
Hpy8I GTNNAC 1 cut(s) 1013
HpyAV CCTTC 2 cut(s) 173, 401
HpyCH4III ACNGT 3 cut(s) 324, 421, 993
HpyCH4IV ACGT 1 cut(s) 980
HpyCH4V TGCA 4 cut(s) 71, 269, 350, 767
HpyF3I CTNAG 5 cut(s) 83, 168, 288, 385, 498
HpySE526I ACGT 1 cut(s) 980
Hsp92II CATG 6 cut(s) 71, 528, 542, 650, 762, 802
HspAI GCGC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 3 cut(s) 259, 300, 933
LmnI GCTCC 2 cut(s) 341, 715
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 1 cut(s) 495
MabI ACCWGGT 1 cut(s) 441
MaeI CTAG 1 cut(s) 146
MaeII ACGT 1 cut(s) 980
MaeIII GTNAC 1 cut(s) 845
MalI GATC 3 cut(s) 261, 302, 935
MboI GATC 3 cut(s) 259, 300, 933
MboII GAAGA 4 cut(s) 34, 214, 449, 826
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 1 cut(s) 259
MlsI TGGCCA 2 cut(s) 633, 645
MluCI AATT 5 cut(s) 316, 627, 656, 682, 688
MluNI TGGCCA 2 cut(s) 633, 645
MlyI GAGTC 2 cut(s) 377, 838
MnlI CCTC 2 cut(s) 297, 404
Mox20I TGGCCA 2 cut(s) 633, 645
MscI TGGCCA 2 cut(s) 633, 645
MseI TTAA 1 cut(s) 566
MslI CAYNNNNRTG 3 cut(s) 609, 639, 926
Msp20I TGGCCA 2 cut(s) 633, 645
MspR9I CCNGG 3 cut(s) 90, 443, 462
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 3 cut(s) 90, 443, 462
NdeII GATC 3 cut(s) 259, 300, 933
NlaIII CATG 6 cut(s) 71, 528, 542, 650, 762, 802
NlaIV GGNNCC 2 cut(s) 261, 906
NmuCI GTSAC 1 cut(s) 845
PagI TCATGA 1 cut(s) 798
PceI AGGCCT 1 cut(s) 88
PfeI GAWTC 2 cut(s) 192, 376
PflMI CCANNNNNTGG 1 cut(s) 256
PfoI TCCNGGA 1 cut(s) 460
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 2 cut(s) 377, 837
PpsI GAGTC 2 cut(s) 377, 837
Psp6I CCWGG 3 cut(s) 88, 441, 460
PspGI CCWGG 3 cut(s) 88, 441, 460
PspN4I GGNNCC 2 cut(s) 261, 906
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 2 cut(s) 702, 979
RsaNI GTAC 2 cut(s) 701, 978
RseI CAYNNNNRTG 3 cut(s) 609, 639, 926
SaqAI TTAA 1 cut(s) 566
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 3 cut(s) 259, 300, 933
SchI GAGTC 2 cut(s) 377, 838
ScrFI CCNGG 3 cut(s) 90, 443, 462
SexAI ACCWGGT 1 cut(s) 441
SfaNI GCATC 1 cut(s) 495
SmiMI CAYNNNNRTG 3 cut(s) 609, 639, 926
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
Sse9I AATT 5 cut(s) 316, 627, 656, 682, 688
SseBI AGGCCT 1 cut(s) 88
SspI AATATT 1 cut(s) 9
SspMI CTAG 1 cut(s) 146
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 3 cut(s) 88, 441, 460
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 3 cut(s) 324, 421, 993
TaiI ACGT 1 cut(s) 983
TaqI TCGA 2 cut(s) 177, 594
TasI AATT 5 cut(s) 316, 627, 656, 682, 688
TatI WGTACW 1 cut(s) 700
TfiI GAWTC 2 cut(s) 192, 376
Tru1I TTAA 1 cut(s) 566
Tru9I TTAA 1 cut(s) 566
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 1 cut(s) 845
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 845
TspDTI ATGAA 6 cut(s) 17, 126, 482, 747, 787, 1011
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 1 cut(s) 256
XapI RAATTY 2 cut(s) 316, 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 633
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.