Rh2CG044100

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0015514
Physical Location & Seq
Reverse (-)
3498601 .. 3501508
2908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG044100.1

Sequence Viewer

Length: 1350 bp
ATGAAAATATTGAGAGCTGCTTCTGTTTTCCTTTGTATTTTCTTCTGCTTCTTTGTTTGCTCATCTCATGCAACAGATACCCTTAGGCCTGGTCAGTATTTGACGAGATATGAAACCTTGGTTTCTGATGGTGGAGTATTTGAGCTAGGCTTTTTCTACAGCCCCACTAAGTCAGTCGAAGGATACTTGGGAATCTGGTTGAAGAATGACAAAAAGAAAAGGCCAGTTTGGGTGGCAAATAGAGATGACCATCTTATGGATCCCTATGCACTTCTCTACATCAACCCTCAGGGAATGGTGATCAGTGATAATAGAAATTCACCGTTTTTTTTCACTTATGGAGCAACTGCAACGACTAATAGGACAAGCGCAAGGATTCTTGACTCAGGAAACCTAATCCTGATGGAAGGAGGAAAGACGGTGTGGCAGAGCTTTGATAACCTGGTAGATACATTTCTTCCTGGAATGAAAATAGGGATGGTTAGCATAACCAATGCTCAGTTGAAGCCTATGTTTCTTTTATCATGGAGAAGCGCACTTGACCCTACTCGTGGTTTTCTTGGTTTAAGCTTGGATAACAGCACAACTCAAGTCGATGTCTGGCACGATGATGGTAAAGAGAAACCAATTGGCCATTGGGACGGCCATGAGTTCAAATTTGTTTTCAAAACCCAATCAGATAATTACAATTTCAGCTATGTACTGAACAGCAAGGAGCGTTATCTTACTTTCAACAACAAAGAAAGTAGTGTATATTCATGGTTTGCATTGGCTTCGGATGGTAAGATACAGGAGTTCATGATGGTAGGACAGAAGATTTCGGTTGTGAGTCATTCGGTATGTGGTGCCTGCTGGTTGGTGTCATTAAGAGATAGGAAGCTGTTGCTGGTCATCAAAGTTGTGGTTCCCTTGACTTTCTCCATTTTGATGTTGATCTTGTTCCTGTATTGTTTATGGAGAAAGCACAACTCTTTAGGAATTTACAGATTCTATATTGGCAGGACAGACAGTGTGAAGCTATTATTATCACAGTTGAGTTCTGATGATAGTGGTAGCAGTTCACCTGTTGTAGAACTTGGAAAGACGAAGGATCATGCTTTGCCAATGCTTAGCTTTTCCTGCATAGTGGCATCCACTAATAATTTCTCAGTTGCTAACAAGCTTGGAGAAGGAGATTATTCTTTGAACTTGCTAGGACATGCCTGGAATTTATGGATATCTGGACGATGTGAAGAGATGATAGATCCAACATTAGCTGATTCAAGTTCAAGGGATGGTCTTGTACTCTGCATTCAGGAAATCCACTTCATGGTTTCCAGTAGTACAGATCTTCAATCTCAGAAAGACTAA

Protein Analysis

449

Amino Acids

50.64

Weight (kDa)

7.12

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 177 3.3e-21 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015514)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03780
malus_domestica MD02G1036400.v1.1 MD15G1176600.v1.1
prunus_persica Prupe.7G239000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0089191
rosa_laevigata RLG00000016003
rosa_multiflora Rmu_sc0008322.1_g000027 Rmu_sc0008909.1_g000009
rosa_roxburghii Rroxscaffold_2G00152160
rosa_rugosa Rorug01G0488300
rosa_samantha Rh2AG043900 Rh2BG042600 Rh2CG044100 Rh2DG042900
rosa_wichuraiana Rw2G003920 Rw2G003930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 845
AccB7I CCANNNNNTGG 2 cut(s) 256, 1309
AclWI GGATC 4 cut(s) 254, 267, 1098, 1238
AcoI YGGCCR 2 cut(s) 631, 643
AcsI RAATTY 4 cut(s) 316, 656, 978, 1207
AfaI GTAC 3 cut(s) 702, 1284, 1324
AfiI CCNNNNNNNGG 3 cut(s) 256, 551, 1309
AgsI TTSAA 9 cut(s) 202, 505, 655, 667, 733, 1186, 1263, 1269, 1334
AjnI CCWGG 4 cut(s) 88, 441, 460, 1202
AlwI GGATC 4 cut(s) 254, 267, 1098, 1238
AoxI GGCC 4 cut(s) 86, 221, 631, 643
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 4 cut(s) 316, 656, 978, 1207
AspLEI GCGC 2 cut(s) 371, 536
AsuHPI GGTGA 3 cut(s) 310, 312, 1053
AxyI CCTNAGG 2 cut(s) 83, 288
BaeI ACNNNNGTAYC 2 cut(s) 779, 812
BalI TGGCCA 1 cut(s) 633
BamHI GGATCC 1 cut(s) 259
BanI GGYRCC 1 cut(s) 845
BauI CACGAG 1 cut(s) 549
BbvI GCAGC 1 cut(s) 4
BccI CCATC 8 cut(s) 122, 258, 397, 472, 605, 773, 796, 1268
BceAI ACGGC 1 cut(s) 658
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciT130I CCWGG 4 cut(s) 90, 443, 462, 1204
BciVI GTATCC 1 cut(s) 176
BclI TGATCA 1 cut(s) 300
BfaI CTAG 2 cut(s) 146, 1193
BfmI CTRYAG 1 cut(s) 157
BfuI GTATCC 1 cut(s) 176
BglII AGATCT 1 cut(s) 1327
BisI GCNGC 1 cut(s) 18
BlpI GCTNAGC 1 cut(s) 1109
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 4 cut(s) 90, 443, 462, 1204
BmiI GGNNCC 3 cut(s) 261, 847, 906
BmrFI CCNGG 4 cut(s) 90, 443, 462, 1204
BmsI GCATC 1 cut(s) 1139
Bpu1102I GCTNAGC 1 cut(s) 1109
BpuEI CTTGAG 1 cut(s) 573
BsaBI GATNNNNATC 2 cut(s) 249, 932
BsaJI CCNNGG 1 cut(s) 117
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 551, 1309
Bse1I ACTGG 2 cut(s) 224, 1317
Bse21I CCTNAGG 2 cut(s) 83, 288
Bse8I GATNNNNATC 2 cut(s) 249, 932
BseBI CCWGG 4 cut(s) 90, 443, 462, 1204
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 4 cut(s) 483, 784, 1130, 1279
BseJI GATNNNNATC 2 cut(s) 249, 932
BseLI CCNNNNNNNGG 3 cut(s) 256, 551, 1309
BseMII CTCAG 4 cut(s) 302, 399, 512, 1161
BseNI ACTGG 2 cut(s) 224, 1317
BseXI GCAGC 1 cut(s) 4
BshFI GGCC 4 cut(s) 88, 223, 633, 645
BshNI GGYRCC 1 cut(s) 845
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 3 cut(s) 256, 551, 1309
BsmFI GGGAC 1 cut(s) 653
BsmI GAATGC 1 cut(s) 1290
BsnI GGCC 4 cut(s) 88, 223, 633, 645
Bsp143I GATC 6 cut(s) 259, 300, 933, 1090, 1243, 1327
Bsp1720I GCTNAGC 1 cut(s) 1109
BspANI GGCC 4 cut(s) 88, 223, 633, 645
BspCNI CTCAG 4 cut(s) 301, 398, 511, 1160
BspHI TCATGA 1 cut(s) 798
BspLI GGNNCC 3 cut(s) 261, 847, 906
BspPI GGATC 4 cut(s) 254, 267, 1098, 1238
BspT107I GGYRCC 1 cut(s) 845
BsrI ACTGG 2 cut(s) 224, 1317
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 6 cut(s) 259, 300, 933, 1090, 1243, 1327
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 117
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 4 cut(s) 90, 443, 462, 1204
Bst4CI ACNGT 4 cut(s) 324, 421, 1010, 1032
Bst6I CTCTTC 1 cut(s) 1227
BstC8I GCNNGC 1 cut(s) 850
BstDEI CTNAG 8 cut(s) 83, 168, 288, 385, 498, 1109, 1147, 1338
BstF5I GGATG 4 cut(s) 483, 784, 1130, 1279
BstHHI GCGC 2 cut(s) 371, 536
BstKTI GATC 6 cut(s) 262, 303, 936, 1093, 1246, 1330
BstMBI GATC 6 cut(s) 259, 300, 933, 1090, 1243, 1327
BstMWI GCNNNNNNNGC 1 cut(s) 1119
BstNI CCWGG 4 cut(s) 90, 443, 462, 1204
BstNSI RCATGY 1 cut(s) 1202
BstSCI CCNGG 4 cut(s) 88, 441, 460, 1202
BstSFI CTRYAG 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 4
BstX2I RGATCY 3 cut(s) 259, 1243, 1327
BstYI RGATCY 3 cut(s) 259, 1243, 1327
Bsu36I CCTNAGG 2 cut(s) 83, 288
BsuI GTATCC 1 cut(s) 176
BsuRI GGCC 4 cut(s) 88, 223, 633, 645
BtsCI GGATG 4 cut(s) 483, 784, 1130, 1279
BtsIMutI CAGTG 2 cut(s) 310, 1015
Cac8I GCNNGC 1 cut(s) 850
CciI TCATGA 1 cut(s) 798
CfoI GCGC 2 cut(s) 371, 536
CsiI ACCWGGT 1 cut(s) 441
Csp6I GTAC 3 cut(s) 701, 1283, 1323
CviAII CATG 8 cut(s) 68, 525, 647, 759, 799, 1094, 1199, 1309
CviQI GTAC 3 cut(s) 701, 1283, 1323
DdeI CTNAG 8 cut(s) 83, 168, 288, 385, 498, 1109, 1147, 1338
DpnI GATC 6 cut(s) 261, 302, 935, 1092, 1245, 1329
DpnII GATC 6 cut(s) 259, 300, 933, 1090, 1243, 1327
EaeI YGGCCR 2 cut(s) 631, 643
Eam1104I CTCTTC 1 cut(s) 1227
EarI CTCTTC 1 cut(s) 1227
Eco130I CCWWGG 1 cut(s) 117
Eco147I AGGCCT 1 cut(s) 88
Eco32I GATATC 1 cut(s) 1218
Eco81I CCTNAGG 2 cut(s) 83, 288
EcoRII CCWGG 4 cut(s) 88, 441, 460, 1202
EcoRV GATATC 1 cut(s) 1218
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 8 cut(s) 71, 528, 650, 762, 802, 1097, 1202, 1312
FaqI GGGAC 1 cut(s) 653
FatI CATG 8 cut(s) 67, 524, 646, 758, 798, 1093, 1198, 1308
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 4 cut(s) 490, 791, 1117, 1286
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 2 cut(s) 146, 1193
GlaI GCGC 2 cut(s) 370, 535
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 4 cut(s) 88, 223, 633, 645
HhaI GCGC 2 cut(s) 371, 536
Hin1II CATG 8 cut(s) 71, 528, 650, 762, 802, 1097, 1202, 1312
Hin6I GCGC 2 cut(s) 369, 534
HinP1I GCGC 2 cut(s) 369, 534
HindIII AAGCTT 2 cut(s) 568, 1160
HinfI GANTC 6 cut(s) 192, 376, 383, 829, 987, 1259
HphI GGTGA 3 cut(s) 310, 312, 1053
Hpy166II GTNNAC 1 cut(s) 1061
Hpy188I TCNGA 5 cut(s) 127, 679, 778, 1042, 1341
Hpy188III TCNNGA 6 cut(s) 380, 387, 400, 799, 1221, 1295
Hpy8I GTNNAC 1 cut(s) 1061
HpyAV CCTTC 4 cut(s) 173, 401, 1081, 1163
HpyCH4III ACNGT 4 cut(s) 324, 421, 1010, 1032
HpyCH4V TGCA 6 cut(s) 71, 269, 350, 767, 1122, 1290
HpyF10VI GCNNNNNNNGC 1 cut(s) 1119
HpyF3I CTNAG 8 cut(s) 83, 168, 288, 385, 498, 1109, 1147, 1338
Hsp92II CATG 8 cut(s) 71, 528, 650, 762, 802, 1097, 1202, 1312
HspAI GCGC 2 cut(s) 369, 534
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 6 cut(s) 259, 300, 933, 1090, 1243, 1327
LmnI GCTCC 2 cut(s) 341, 715
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 1 cut(s) 1139
MabI ACCWGGT 1 cut(s) 441
MaeI CTAG 2 cut(s) 146, 1193
MalI GATC 6 cut(s) 261, 302, 935, 1092, 1245, 1329
MboI GATC 6 cut(s) 259, 300, 933, 1090, 1243, 1327
MboII GAAGA 6 cut(s) 34, 214, 449, 826, 1244, 1322
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 3 cut(s) 259, 1243, 1327
MlsI TGGCCA 1 cut(s) 633
MluCI AATT 8 cut(s) 316, 627, 656, 682, 688, 978, 1141, 1207
MluNI TGGCCA 1 cut(s) 633
MlyI GAGTC 2 cut(s) 377, 838
MmeI TCCRAC 1 cut(s) 1271
MnlI CCTC 2 cut(s) 297, 404
Mox20I TGGCCA 1 cut(s) 633
MscI TGGCCA 1 cut(s) 633
MseI TTAA 2 cut(s) 566, 866
MslI CAYNNNNRTG 2 cut(s) 609, 926
Msp20I TGGCCA 1 cut(s) 633
MspR9I CCNGG 4 cut(s) 90, 443, 462, 1204
MunI CAATTG 1 cut(s) 627
Mva1269I GAATGC 1 cut(s) 1290
MvaI CCWGG 4 cut(s) 90, 443, 462, 1204
MwoI GCNNNNNNNGC 1 cut(s) 1119
NdeII GATC 6 cut(s) 259, 300, 933, 1090, 1243, 1327
NlaIII CATG 8 cut(s) 71, 528, 650, 762, 802, 1097, 1202, 1312
NlaIV GGNNCC 3 cut(s) 261, 847, 906
NspI RCATGY 1 cut(s) 1202
PagI TCATGA 1 cut(s) 798
PceI AGGCCT 1 cut(s) 88
PctI GAATGC 1 cut(s) 1290
PfeI GAWTC 4 cut(s) 192, 376, 987, 1259
PflMI CCANNNNNTGG 2 cut(s) 256, 1309
PfoI TCCNGGA 1 cut(s) 460
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 2 cut(s) 377, 837
PpsI GAGTC 2 cut(s) 377, 837
Psp6I CCWGG 4 cut(s) 88, 441, 460, 1202
PspGI CCWGG 4 cut(s) 88, 441, 460, 1202
PspN4I GGNNCC 3 cut(s) 261, 847, 906
PsuI RGATCY 3 cut(s) 259, 1243, 1327
RsaI GTAC 3 cut(s) 702, 1284, 1324
RsaNI GTAC 3 cut(s) 701, 1283, 1323
RseI CAYNNNNRTG 2 cut(s) 609, 926
SaqAI TTAA 2 cut(s) 566, 866
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 6 cut(s) 259, 300, 933, 1090, 1243, 1327
SchI GAGTC 2 cut(s) 377, 838
ScrFI CCNGG 4 cut(s) 90, 443, 462, 1204
SexAI ACCWGGT 1 cut(s) 441
SfaNI GCATC 1 cut(s) 1139
SfcI CTRYAG 1 cut(s) 157
SmiMI CAYNNNNRTG 2 cut(s) 609, 926
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
Sse9I AATT 8 cut(s) 316, 627, 656, 682, 688, 978, 1141, 1207
SseBI AGGCCT 1 cut(s) 88
SspI AATATT 1 cut(s) 9
SspMI CTAG 2 cut(s) 146, 1193
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 4 cut(s) 88, 441, 460, 1202
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 4 cut(s) 324, 421, 1010, 1032
TaqI TCGA 2 cut(s) 177, 594
TasI AATT 8 cut(s) 316, 627, 656, 682, 688, 978, 1141, 1207
TatI WGTACW 3 cut(s) 700, 1282, 1322
TfiI GAWTC 4 cut(s) 192, 376, 987, 1259
Tru1I TTAA 2 cut(s) 566, 866
Tru9I TTAA 2 cut(s) 566, 866
TscAI CASTG 2 cut(s) 310, 1015
TseI GCWGC 1 cut(s) 17
TspDTI ATGAA 6 cut(s) 17, 126, 482, 747, 787, 1297
TspRI CASTG 2 cut(s) 310, 1015
Van91I CCANNNNNTGG 2 cut(s) 256, 1309
XapI RAATTY 4 cut(s) 316, 656, 978, 1207
XceI RCATGY 1 cut(s) 1202
XcmI CCANNNNNNNNNTGG 1 cut(s) 633
XspI CTAG 2 cut(s) 146, 1193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.