Rh2DG042900

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0015514
Physical Location & Seq
Reverse (-)
2982026 .. 2983334
1309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG042900.1

Sequence Viewer

Length: 1212 bp
ATGAAAATATTGAGAGCTGCTTCTGTTTTCCTTGGTATTTTCTTCTGCTTCTTTGTTTGCTCATCTCATGCAACAGATACCCTTAGGCCTGGTCAGTATTTGACGAGATATGAAACCTTGGTTTCTGATGGTGGAGTATTTGAGCTAGGCTTTTTCTACAGCCCCACTAAGTCAGTCGAAGGATACTTGGGAATCTGGTTGAAGAATGACAAAAAGAAAAGGCCAGTTTGGGTGGCAAATAGAGATGACCATCTTATGGATCCCTATGCACTTCTCTACATCAACCCTCAGGGAATGGTGATCAGTGATAATAGAAATTCACCGTTTTTTTTCACTTATGGAGCAACTGCAACGACTAATAGGACAAAAGCAAGGATTCTTGACTCAGGAAACCTAATCCTGATGGAAGGAGGAAAGACGGTGTGGCAGAGCTTTGATAACCTGGTAGATACATTTCTTCCTGGAATGAAAATAGGGATGGTTAGCACAACCAATGCTCAGTTGAAGCCTATGTTTCTTTTATCATGGAGAAGCGCACATGACCCTACTCGTGGTTTTCTTGGTTTAAGCTTGGATAACAGCACAACTCAAGTCGATGTCTGGCACGATGATGGTAAAGAGAAACCAATTGGCCATTGGGATGGCCATGAGTTCAAATTTGTTTTCAAAACCCAATCAGATAATTACAATTTCAGCTATGTACTGAACAGCAAGGAGCGTTATCTTACTTTCAACAACAAAGAAAGTAGTGTATATTCATGGTTTGCATTGGCTTCGGATGGTAAGATACAGGAGTTCATGATGGCAGGACAGAAGATTTCGGTTGTGAGTCATTCGGTATGTGGTGACTGCTGGTTGGTGTCATCAAGAGATAGGAAGCTGTTGCTGGTCATCAAAGTTGTGGTTCCCTTGACTTTCTCCATTTTGATGTTGATCTTGTTCCTGTATTGTTTATGGAGAAAGCATAACTCTTTAGGAATTTACAGATTCTATATTGGCAGGACAGACAGTGTGAAGCTATTATTATCACAGTTGAGTTCTGATGATAGTGGTAGCAGTTCACCTGTTGTAGAACTTGGAAAGACGAAGGATCATGCTTTGCCAATGCTTAGCTTTTCCTGCATAGTGGCATCCACTAATAATTTCTCAGTTGCTAACAAGCTTGGAGAAGGAGGTTATGGGCCTGTTTATAAGGTGTTTGTTGCCTTGTAG

Protein Analysis

403

Amino Acids

45.42

Weight (kDa)

8.93

Isoelectric Point (pI)

28.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 177 2.8e-21 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015514)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03780
malus_domestica MD02G1036400.v1.1 MD15G1176600.v1.1
prunus_persica Prupe.7G239000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0089191
rosa_laevigata RLG00000016003
rosa_multiflora Rmu_sc0008322.1_g000027 Rmu_sc0008909.1_g000009
rosa_roxburghii Rroxscaffold_2G00152160
rosa_rugosa Rorug01G0488300
rosa_samantha Rh2AG043900 Rh2BG042600 Rh2CG044100 Rh2DG042900
rosa_wichuraiana Rw2G003920 Rw2G003930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1191
AccB7I CCANNNNNTGG 1 cut(s) 256
AclWI GGATC 3 cut(s) 254, 267, 1098
AcoI YGGCCR 2 cut(s) 631, 643
AcsI RAATTY 3 cut(s) 316, 656, 978
AfaI GTAC 1 cut(s) 702
AfiI CCNNNNNNNGG 2 cut(s) 256, 551
AgsI TTSAA 5 cut(s) 202, 505, 655, 667, 733
AjnI CCWGG 3 cut(s) 88, 441, 460
AluBI AGCT 9 cut(s) 17, 145, 432, 570, 696, 880, 1018, 1113, 1162
AluI AGCT 9 cut(s) 17, 145, 432, 570, 696, 880, 1018, 1113, 1162
AlwI GGATC 3 cut(s) 254, 267, 1098
AoxI GGCC 5 cut(s) 86, 221, 631, 643, 1181
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 3 cut(s) 316, 656, 978
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 1 cut(s) 1181
AsuHPI GGTGA 4 cut(s) 310, 312, 857, 1053
AxyI CCTNAGG 2 cut(s) 83, 288
BaeI ACNNNNGTAYC 2 cut(s) 779, 812
BalI TGGCCA 2 cut(s) 633, 645
BamHI GGATCC 1 cut(s) 259
BauI CACGAG 1 cut(s) 549
BbvI GCAGC 1 cut(s) 4
BccI CCATC 8 cut(s) 122, 258, 397, 472, 605, 635, 773, 796
BcgI CGANNNNNNTGC 2 cut(s) 756, 790
BciT130I CCWGG 3 cut(s) 90, 443, 462
BciVI GTATCC 1 cut(s) 176
BclI TGATCA 1 cut(s) 300
BfaI CTAG 1 cut(s) 146
BfmI CTRYAG 1 cut(s) 157
BfuI GTATCC 1 cut(s) 176
BisI GCNGC 1 cut(s) 18
BlpI GCTNAGC 1 cut(s) 1109
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 3 cut(s) 90, 443, 462
BmgT120I GGNCC 1 cut(s) 1181
BmiI GGNNCC 2 cut(s) 261, 906
BmrFI CCNGG 3 cut(s) 90, 443, 462
BmsI GCATC 1 cut(s) 1139
Bpu1102I GCTNAGC 1 cut(s) 1109
BpuEI CTTGAG 1 cut(s) 573
BsaBI GATNNNNATC 2 cut(s) 249, 932
BsaJI CCNNGG 2 cut(s) 31, 117
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 551
Bse1I ACTGG 1 cut(s) 224
Bse21I CCTNAGG 2 cut(s) 83, 288
Bse8I GATNNNNATC 2 cut(s) 249, 932
BseBI CCWGG 3 cut(s) 90, 443, 462
BseDI CCNNGG 2 cut(s) 31, 117
BseGI GGATG 4 cut(s) 483, 646, 784, 1130
BseJI GATNNNNATC 2 cut(s) 249, 932
BseLI CCNNNNNNNGG 2 cut(s) 256, 551
BseMII CTCAG 4 cut(s) 302, 399, 512, 1161
BseNI ACTGG 1 cut(s) 224
BseXI GCAGC 1 cut(s) 4
BshFI GGCC 5 cut(s) 88, 223, 633, 645, 1183
BslI CCNNNNNNNGG 2 cut(s) 256, 551
BsnI GGCC 5 cut(s) 88, 223, 633, 645, 1183
Bsp143I GATC 4 cut(s) 259, 300, 933, 1090
Bsp1720I GCTNAGC 1 cut(s) 1109
BspANI GGCC 5 cut(s) 88, 223, 633, 645, 1183
BspCNI CTCAG 4 cut(s) 301, 398, 511, 1160
BspHI TCATGA 1 cut(s) 798
BspLI GGNNCC 2 cut(s) 261, 906
BspPI GGATC 3 cut(s) 254, 267, 1098
BsrI ACTGG 1 cut(s) 224
BssECI CCNNGG 2 cut(s) 31, 117
BssMI GATC 4 cut(s) 259, 300, 933, 1090
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 2 cut(s) 31, 117
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 3 cut(s) 90, 443, 462
Bst4CI ACNGT 4 cut(s) 324, 421, 1010, 1032
BstDEI CTNAG 7 cut(s) 83, 168, 288, 385, 498, 1109, 1147
BstF5I GGATG 4 cut(s) 483, 646, 784, 1130
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 4 cut(s) 262, 303, 936, 1093
BstMBI GATC 4 cut(s) 259, 300, 933, 1090
BstMWI GCNNNNNNNGC 1 cut(s) 1119
BstNI CCWGG 3 cut(s) 90, 443, 462
BstSCI CCNGG 3 cut(s) 88, 441, 460
BstSFI CTRYAG 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 4
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 1 cut(s) 641
BstYI RGATCY 1 cut(s) 259
Bsu36I CCTNAGG 2 cut(s) 83, 288
BsuI GTATCC 1 cut(s) 176
BsuRI GGCC 5 cut(s) 88, 223, 633, 645, 1183
BtsCI GGATG 4 cut(s) 483, 646, 784, 1130
BtsIMutI CAGTG 2 cut(s) 310, 1015
CciI TCATGA 1 cut(s) 798
CfoI GCGC 1 cut(s) 536
Cfr13I GGNCC 1 cut(s) 1181
CsiI ACCWGGT 1 cut(s) 441
Csp6I GTAC 1 cut(s) 701
CviAII CATG 7 cut(s) 68, 525, 539, 647, 759, 799, 1094
CviQI GTAC 1 cut(s) 701
DdeI CTNAG 7 cut(s) 83, 168, 288, 385, 498, 1109, 1147
DpnI GATC 4 cut(s) 261, 302, 935, 1092
DpnII GATC 4 cut(s) 259, 300, 933, 1090
EaeI YGGCCR 2 cut(s) 631, 643
Eco130I CCWWGG 2 cut(s) 31, 117
Eco147I AGGCCT 1 cut(s) 88
Eco81I CCTNAGG 2 cut(s) 83, 288
EcoRII CCWGG 3 cut(s) 88, 441, 460
EcoT14I CCWWGG 2 cut(s) 31, 117
ErhI CCWWGG 2 cut(s) 31, 117
FaeI CATG 7 cut(s) 71, 528, 542, 650, 762, 802, 1097
FatI CATG 7 cut(s) 67, 524, 538, 646, 758, 798, 1093
FbaI TGATCA 1 cut(s) 300
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 4 cut(s) 490, 653, 791, 1117
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 1 cut(s) 146
GlaI GCGC 1 cut(s) 535
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 5 cut(s) 88, 223, 633, 645, 1183
HhaI GCGC 1 cut(s) 536
Hin1II CATG 7 cut(s) 71, 528, 542, 650, 762, 802, 1097
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HindIII AAGCTT 2 cut(s) 568, 1160
HinfI GANTC 5 cut(s) 192, 376, 383, 829, 987
HphI GGTGA 4 cut(s) 310, 312, 857, 1053
Hpy166II GTNNAC 1 cut(s) 1061
Hpy188I TCNGA 4 cut(s) 127, 679, 778, 1042
Hpy188III TCNNGA 5 cut(s) 380, 387, 400, 799, 867
Hpy8I GTNNAC 1 cut(s) 1061
HpyAV CCTTC 4 cut(s) 173, 401, 1081, 1163
HpyCH4III ACNGT 4 cut(s) 324, 421, 1010, 1032
HpyCH4V TGCA 5 cut(s) 71, 269, 350, 767, 1122
HpyF10VI GCNNNNNNNGC 1 cut(s) 1119
HpyF3I CTNAG 7 cut(s) 83, 168, 288, 385, 498, 1109, 1147
Hsp92II CATG 7 cut(s) 71, 528, 542, 650, 762, 802, 1097
HspAI GCGC 1 cut(s) 534
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 4 cut(s) 259, 300, 933, 1090
LmnI GCTCC 2 cut(s) 341, 715
Lsp1109I GCAGC 1 cut(s) 4
LweI GCATC 1 cut(s) 1139
MabI ACCWGGT 1 cut(s) 441
MaeI CTAG 1 cut(s) 146
MaeIII GTNAC 1 cut(s) 845
MalI GATC 4 cut(s) 261, 302, 935, 1092
MboI GATC 4 cut(s) 259, 300, 933, 1090
MboII GAAGA 4 cut(s) 34, 214, 449, 826
MfeI CAATTG 1 cut(s) 627
MflI RGATCY 1 cut(s) 259
MlsI TGGCCA 2 cut(s) 633, 645
MluCI AATT 7 cut(s) 316, 627, 656, 682, 688, 978, 1141
MluNI TGGCCA 2 cut(s) 633, 645
MlyI GAGTC 2 cut(s) 377, 838
MnlI CCTC 3 cut(s) 297, 404, 1166
Mox20I TGGCCA 2 cut(s) 633, 645
MscI TGGCCA 2 cut(s) 633, 645
MseI TTAA 1 cut(s) 566
MslI CAYNNNNRTG 3 cut(s) 609, 639, 926
Msp20I TGGCCA 2 cut(s) 633, 645
MspR9I CCNGG 3 cut(s) 90, 443, 462
MunI CAATTG 1 cut(s) 627
MvaI CCWGG 3 cut(s) 90, 443, 462
MwoI GCNNNNNNNGC 1 cut(s) 1119
NdeII GATC 4 cut(s) 259, 300, 933, 1090
NlaIII CATG 7 cut(s) 71, 528, 542, 650, 762, 802, 1097
NlaIV GGNNCC 2 cut(s) 261, 906
NmuCI GTSAC 1 cut(s) 845
PagI TCATGA 1 cut(s) 798
PceI AGGCCT 1 cut(s) 88
PfeI GAWTC 3 cut(s) 192, 376, 987
PflMI CCANNNNNTGG 1 cut(s) 256
PfoI TCCNGGA 1 cut(s) 460
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 2 cut(s) 377, 837
PpsI GAGTC 2 cut(s) 377, 837
PsiI TTATAA 1 cut(s) 1191
Psp6I CCWGG 3 cut(s) 88, 441, 460
PspGI CCWGG 3 cut(s) 88, 441, 460
PspN4I GGNNCC 2 cut(s) 261, 906
PspPI GGNCC 1 cut(s) 1181
PsuI RGATCY 1 cut(s) 259
RsaI GTAC 1 cut(s) 702
RsaNI GTAC 1 cut(s) 701
RseI CAYNNNNRTG 3 cut(s) 609, 639, 926
SaqAI TTAA 1 cut(s) 566
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 4 cut(s) 259, 300, 933, 1090
Sau96I GGNCC 1 cut(s) 1181
SchI GAGTC 2 cut(s) 377, 838
ScrFI CCNGG 3 cut(s) 90, 443, 462
SexAI ACCWGGT 1 cut(s) 441
SfaNI GCATC 1 cut(s) 1139
SfcI CTRYAG 1 cut(s) 157
SmiMI CAYNNNNRTG 3 cut(s) 609, 639, 926
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
Sse9I AATT 7 cut(s) 316, 627, 656, 682, 688, 978, 1141
SseBI AGGCCT 1 cut(s) 88
SspI AATATT 1 cut(s) 9
SspMI CTAG 1 cut(s) 146
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 3 cut(s) 88, 441, 460
StyI CCWWGG 2 cut(s) 31, 117
TaaI ACNGT 4 cut(s) 324, 421, 1010, 1032
TaqI TCGA 2 cut(s) 177, 594
TasI AATT 7 cut(s) 316, 627, 656, 682, 688, 978, 1141
TatI WGTACW 1 cut(s) 700
TfiI GAWTC 3 cut(s) 192, 376, 987
Tru1I TTAA 1 cut(s) 566
Tru9I TTAA 1 cut(s) 566
TscAI CASTG 2 cut(s) 310, 1015
TseFI GTSAC 1 cut(s) 845
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 845
TspDTI ATGAA 5 cut(s) 17, 126, 482, 747, 787
TspRI CASTG 2 cut(s) 310, 1015
Van91I CCANNNNNTGG 1 cut(s) 256
XapI RAATTY 3 cut(s) 316, 656, 978
XcmI CCANNNNNNNNNTGG 1 cut(s) 633
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.