Rw2G003930

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015514
Physical Location & Seq
Forward (+)
3687872 .. 3689332
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003930.1

Sequence Viewer

Length: 987 bp
ATGCTTAGCTTTTCCTGCATAGTGGCATCCACTAATAATTTCTCAGTTGCTAACAAGCTTGGAGAAGGAGGTTATGGGCCTGTTTATAAGGGTAACTTACTGCAACACGACATTGCGGTCAAAAGGCTTTCAAAAAGATCTGGACAAGGATTAGCGGAGTTCAAAAATGAGGTGCAGTTAATATCTCAGCTCCAGCACAGAAATCTTGTTAAGATTTTGGGCTGTTGCATTCAACGAGAAGAAAAAATATTAATATACGAGTATATGTCCAACAAAAGTTTGGATTCCTTCATTTTTGATCCAATTAAGCGAAGACAACTGAATTGGAGACAGCGCATACACATAATCGAAGGGATTGCTCAAGGGCTTCTTTATCTTCACAAGTACTCTAGACTAAGAATTATTCATCGAGACCTCAAAACTAGCAACATCTTATTGGATGCCTATATGAACCCCAAGATATCAGACTTTGGCTTGGCCAGAATCTTATCCGGGAATGAATGTGGGGCTGAAACAAAGCGGATTGCTGGAACATTCGGTTATATGTCTCCCGAGTATGTATTACAAGGCCTTTTGTCAACAAAATCGGATGTATTCAGCTTTGGAGTCATTGTGTTGGAGATTATGAGTGGCATGAAGAATGCAACCTTTTATGAGTCAGATTATTCTTTGAACTTGCTAGGACATGCCTGGAATTTATGGATATCTGGACGATGTGAAGAGATGATAGATCCAACATTAGCTGATTCAAGTTCAAGGGATGGTCTTGTACTCTGCATTCAGGTAGGTCTTTTATGCGTTCAAGATTGTGCTGAAGATAGACCAACCATGTCAGAAGTTGTTTCAATGTTGAGCACTGAAGGAGCTAGTTTACCTAAGCCAAAGCAGCCTATGTATTCTAACTTAATGTCAGAAGTAGATAAAGCTTTAAGAAGTGGAGTCCCTTCTGAGAATCTCTTGACATATTCATCAATAGTAGCAAGATAG

Protein Analysis

328

Amino Acids

36.72

Weight (kDa)

8.33

Isoelectric Point (pI)

57.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 281 2.4e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 16 - 214 5.5e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015514)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03780
malus_domestica MD02G1036400.v1.1 MD15G1176600.v1.1
prunus_persica Prupe.7G239000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0089191
rosa_laevigata RLG00000016003
rosa_multiflora Rmu_sc0008322.1_g000027 Rmu_sc0008909.1_g000009
rosa_roxburghii Rroxscaffold_2G00152160
rosa_rugosa Rorug01G0488300
rosa_samantha Rh2AG043900 Rh2BG042600 Rh2CG044100 Rh2DG042900
rosa_wichuraiana Rw2G003920 Rw2G003930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 87
AasI GACNNNNNNGTC 1 cut(s) 116
AciI CCGC 3 cut(s) 116, 155, 520
AclWI GGATC 2 cut(s) 293, 725
AcoI YGGCCR 1 cut(s) 477
AcsI RAATTY 1 cut(s) 694
AcuI CTGAAG 2 cut(s) 834, 879
AfaI GTAC 2 cut(s) 386, 771
AgsI TTSAA 8 cut(s) 132, 163, 233, 673, 750, 756, 803, 846
AjnI CCWGG 1 cut(s) 689
AluBI AGCT 7 cut(s) 9, 58, 190, 600, 743, 866, 926
AluI AGCT 7 cut(s) 9, 58, 190, 600, 743, 866, 926
Alw21I GWGCWC 1 cut(s) 857
Alw26I GTCTC 3 cut(s) 322, 405, 552
AlwI GGATC 2 cut(s) 293, 725
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 3 cut(s) 77, 477, 568
ApeKI GCWGC 1 cut(s) 886
ApoI RAATTY 1 cut(s) 694
AseI ATTAAT 1 cut(s) 251
AspLEI GCGC 1 cut(s) 336
AspS9I GGNCC 1 cut(s) 77
AsuC2I CCSGG 1 cut(s) 493
AvaI CYCGRG 1 cut(s) 551
BalI TGGCCA 1 cut(s) 479
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 857
BbvI GCAGC 1 cut(s) 898
BccI CCATC 1 cut(s) 755
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 1 cut(s) 691
BcnI CCSGG 1 cut(s) 493
BcoDI GTCTC 3 cut(s) 322, 405, 552
BfaI CTAG 4 cut(s) 390, 423, 680, 867
BglII AGATCT 1 cut(s) 137
BisI GCNGC 1 cut(s) 887
BlpI GCTNAGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 888
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 2 cut(s) 493, 691
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 1 cut(s) 77
BmrFI CCNGG 2 cut(s) 493, 691
BmsI GCATC 2 cut(s) 35, 430
BpiI GAAGAC 1 cut(s) 319
BpmI CTGGAG 1 cut(s) 176
Bpu10I CCTNAGC 1 cut(s) 876
Bpu1102I GCTNAGC 1 cut(s) 5
BpuEI CTTGAG 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 493
BsaI GGTCTC 1 cut(s) 405
BsaXI ACNNNNNCTCC 2 cut(s) 597, 627
Bse3DI GCAATG 1 cut(s) 111
BseBI CCWGG 1 cut(s) 691
BseGI GGATG 4 cut(s) 26, 445, 595, 766
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 3 cut(s) 57, 200, 939
BseXI GCAGC 1 cut(s) 898
BsgI GTGCAG 1 cut(s) 194
BshFI GGCC 3 cut(s) 79, 479, 570
BsiHKAI GWGCWC 1 cut(s) 857
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 1 cut(s) 492
BslFI GGGAC 1 cut(s) 926
BsmAI GTCTC 3 cut(s) 322, 405, 552
BsmFI GGGAC 1 cut(s) 926
BsmI GAATGC 3 cut(s) 228, 646, 777
BsnI GGCC 3 cut(s) 79, 479, 570
Bso31I GGTCTC 1 cut(s) 405
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 857
Bsp143I GATC 3 cut(s) 137, 298, 730
Bsp1720I GCTNAGC 1 cut(s) 5
BspACI CCGC 3 cut(s) 116, 155, 520
BspANI GGCC 3 cut(s) 79, 479, 570
BspCNI CTCAG 3 cut(s) 56, 199, 940
BspPI GGATC 2 cut(s) 293, 725
BspTNI GGTCTC 1 cut(s) 405
BsrDI GCAATG 1 cut(s) 111
BssMI GATC 3 cut(s) 137, 298, 730
Bst2UI CCWGG 1 cut(s) 691
Bst6I CTCTTC 1 cut(s) 714
BstDEI CTNAG 6 cut(s) 5, 43, 186, 395, 876, 948
BstF5I GGATG 4 cut(s) 26, 445, 595, 766
BstHHI GCGC 1 cut(s) 336
BstKTI GATC 3 cut(s) 140, 301, 733
BstMAI GTCTC 3 cut(s) 322, 405, 552
BstMBI GATC 3 cut(s) 137, 298, 730
BstMWI GCNNNNNNNGC 2 cut(s) 15, 886
BstNI CCWGG 1 cut(s) 691
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 2 cut(s) 491, 689
BstV1I GCAGC 1 cut(s) 898
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 2 cut(s) 137, 730
BstYI RGATCY 2 cut(s) 137, 730
BsuRI GGCC 3 cut(s) 79, 479, 570
BtsCI GGATG 4 cut(s) 26, 445, 595, 766
BtsIMutI CAGTG 1 cut(s) 855
CfoI GCGC 1 cut(s) 336
Cfr13I GGNCC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 385, 770
CviAII CATG 3 cut(s) 634, 686, 829
CviQI GTAC 2 cut(s) 385, 770
DdeI CTNAG 6 cut(s) 5, 43, 186, 395, 876, 948
DpnI GATC 3 cut(s) 139, 300, 732
DpnII GATC 3 cut(s) 137, 298, 730
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 477
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
Eco147I AGGCCT 1 cut(s) 570
Eco31I GGTCTC 1 cut(s) 405
Eco32I GATATC 2 cut(s) 462, 705
Eco57I CTGAAG 2 cut(s) 834, 879
Eco88I CYCGRG 1 cut(s) 551
EcoRII CCWGG 1 cut(s) 689
EcoRV GATATC 2 cut(s) 462, 705
FaeI CATG 3 cut(s) 637, 689, 832
FalI AAGNNNNNCTT 4 cut(s) 80, 112, 354, 386
FaqI GGGAC 1 cut(s) 926
FatI CATG 3 cut(s) 633, 685, 828
Fnu4HI GCNGC 1 cut(s) 887
FokI GGATG 4 cut(s) 13, 452, 602, 773
Fsp4HI GCNGC 1 cut(s) 887
FspBI CTAG 4 cut(s) 390, 423, 680, 867
GlaI GCGC 1 cut(s) 335
GluI GCNGC 1 cut(s) 887
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 3 cut(s) 79, 479, 570
HapII CCGG 1 cut(s) 492
HhaI GCGC 1 cut(s) 336
Hin1II CATG 3 cut(s) 637, 689, 832
Hin6I GCGC 1 cut(s) 334
HinP1I GCGC 1 cut(s) 334
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 2 cut(s) 56, 924
HinfI GANTC 7 cut(s) 284, 483, 606, 656, 746, 939, 952
HpaII CCGG 1 cut(s) 492
Hpy166II GTNNAC 2 cut(s) 579, 872
Hpy188I TCNGA 6 cut(s) 466, 589, 661, 835, 913, 949
Hpy188III TCNNGA 7 cut(s) 141, 390, 410, 551, 708, 803, 958
Hpy8I GTNNAC 2 cut(s) 579, 872
HpyAV CCTTC 5 cut(s) 59, 298, 344, 854, 954
HpyCH4V TGCA 6 cut(s) 18, 103, 175, 228, 644, 777
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 886
HpyF3I CTNAG 6 cut(s) 5, 43, 186, 395, 876, 948
Hsp92II CATG 3 cut(s) 637, 689, 832
HspAI GCGC 1 cut(s) 334
Kzo9I GATC 3 cut(s) 137, 298, 730
LmnI GCTCC 2 cut(s) 195, 863
Lsp1109I GCAGC 1 cut(s) 898
LweI GCATC 2 cut(s) 35, 430
MaeI CTAG 4 cut(s) 390, 423, 680, 867
MaeIII GTNAC 1 cut(s) 92
MalI GATC 3 cut(s) 139, 300, 732
MboI GATC 3 cut(s) 137, 298, 730
MboII GAAGA 6 cut(s) 251, 324, 368, 649, 731, 827
MflI RGATCY 2 cut(s) 137, 730
MhlI GDGCHC 1 cut(s) 857
MlsI TGGCCA 1 cut(s) 479
MluCI AATT 5 cut(s) 37, 303, 322, 399, 694
MluNI TGGCCA 1 cut(s) 479
MlyI GAGTC 3 cut(s) 615, 665, 948
MmeI TCCRAC 3 cut(s) 294, 597, 758
MnlI CCTC 3 cut(s) 62, 163, 425
Mox20I TGGCCA 1 cut(s) 479
MscI TGGCCA 1 cut(s) 479
MseI TTAA 6 cut(s) 179, 210, 251, 306, 905, 929
Msp20I TGGCCA 1 cut(s) 479
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 2 cut(s) 493, 691
Mva1269I GAATGC 3 cut(s) 228, 646, 777
MvaI CCWGG 1 cut(s) 691
MwoI GCNNNNNNNGC 2 cut(s) 15, 886
NciI CCSGG 1 cut(s) 493
NdeII GATC 3 cut(s) 137, 298, 730
NlaIII CATG 3 cut(s) 637, 689, 832
NspI RCATGY 1 cut(s) 689
PceI AGGCCT 1 cut(s) 570
PctI GAATGC 3 cut(s) 228, 646, 777
PfeI GAWTC 4 cut(s) 284, 483, 746, 952
PfoI TCCNGGA 1 cut(s) 491
PkrI GCNGC 1 cut(s) 888
PleI GAGTC 3 cut(s) 614, 664, 947
PpsI GAGTC 3 cut(s) 614, 664, 947
PshBI ATTAAT 1 cut(s) 251
PsiI TTATAA 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 689
PspGI CCWGG 1 cut(s) 689
PspPI GGNCC 1 cut(s) 77
PsuI RGATCY 2 cut(s) 137, 730
RsaI GTAC 2 cut(s) 386, 771
RsaNI GTAC 2 cut(s) 385, 770
SaqAI TTAA 6 cut(s) 179, 210, 251, 306, 905, 929
SatI GCNGC 1 cut(s) 887
Sau3AI GATC 3 cut(s) 137, 298, 730
Sau96I GGNCC 1 cut(s) 77
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 3 cut(s) 615, 665, 948
ScrFI CCNGG 2 cut(s) 493, 691
SduI GDGCHC 1 cut(s) 857
SfaNI GCATC 2 cut(s) 35, 430
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
Sse9I AATT 5 cut(s) 37, 303, 322, 399, 694
SseBI AGGCCT 1 cut(s) 570
SsiI CCGC 3 cut(s) 116, 155, 520
SspI AATATT 1 cut(s) 249
SspMI CTAG 4 cut(s) 390, 423, 680, 867
StuI AGGCCT 1 cut(s) 570
StyD4I CCNGG 2 cut(s) 491, 689
TaqI TCGA 2 cut(s) 348, 409
TasI AATT 5 cut(s) 37, 303, 322, 399, 694
TatI WGTACW 2 cut(s) 384, 769
TfiI GAWTC 4 cut(s) 284, 483, 746, 952
Tru1I TTAA 6 cut(s) 179, 210, 251, 306, 905, 929
Tru9I TTAA 6 cut(s) 179, 210, 251, 306, 905, 929
TscAI CASTG 1 cut(s) 862
TseI GCWGC 1 cut(s) 886
TspDTI ATGAA 6 cut(s) 280, 395, 464, 513, 650, 957
TspRI CASTG 1 cut(s) 862
VspI ATTAAT 1 cut(s) 251
XapI RAATTY 1 cut(s) 694
XbaI TCTAGA 1 cut(s) 389
XceI RCATGY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 277
XspI CTAG 4 cut(s) 390, 423, 680, 867
ZrmI AGTACT 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.