MD02G1037700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004798
Physical Location & Seq
Reverse (-)
2848566 .. 2848931
366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1037700.v1.1.491

Sequence Viewer

Length: 366 bp
ATGTCCACTAATCTCATCAACTTCCGTCCAATCCTAACCGCGCCGTGCGCCGCCGGACACCGAAGGCCCAGCTCAGTTGGCGGGAAAGGCTCGCCGTCCAAGTGGTGGACCCCGATCTTCGGGTGGTCCTCCGAACCCGACTACATTGACCCGGAGGCAAAGACGGAGTCCGACGACAAGGACGAGGGCGGAGCAGCCGACTCGAAGCAGAGGCGATCGCGGTTCGCGCCCGGCTGCTTCACGGAGGAGAAGGCGCGGCAGCTCCGGATCAAGACGATCGAGATGGAGACGTATCATGACGCGATGTATCACTCGTCGATCGCCAACAGACTCGCCTCCGATTTCAGGAACCGGTCCGATCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

13.61

Weight (kDa)

7.81

Isoelectric Point (pI)

71.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 105
AccII CGCG 5 cut(s) 41, 220, 227, 256, 302
AccIII TCCGGA 1 cut(s) 264
AciI CCGC 6 cut(s) 39, 51, 81, 189, 220, 256
AclWI GGATC 1 cut(s) 275
AfiI CCNNNNNNNGG 3 cut(s) 105, 119, 345
AgeI ACCGGT 1 cut(s) 351
AluBI AGCT 2 cut(s) 72, 262
AluI AGCT 2 cut(s) 72, 262
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 1 cut(s) 275
Aor13HI TCCGGA 1 cut(s) 264
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 3 cut(s) 194, 234, 259
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AsiGI ACCGGT 1 cut(s) 351
AsiSI GCGATCGC 1 cut(s) 218
AspLEI GCGC 4 cut(s) 43, 50, 229, 256
AspS9I GGNCC 4 cut(s) 66, 108, 126, 354
AsuC2I CCSGG 2 cut(s) 152, 231
AvaII GGWCC 3 cut(s) 108, 126, 354
BbvI GCAGC 3 cut(s) 206, 221, 271
BccI CCATC 1 cut(s) 277
BceAI ACGGC 2 cut(s) 28, 79
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BcnI CCSGG 2 cut(s) 152, 231
BcoDI GTCTC 1 cut(s) 281
BisI GCNGC 5 cut(s) 51, 195, 235, 257, 260
BlsI GCNGC 5 cut(s) 52, 196, 236, 258, 261
Bme1390I CCNGG 2 cut(s) 152, 231
Bme18I GGWCC 3 cut(s) 108, 126, 354
BmgT120I GGNCC 4 cut(s) 66, 108, 126, 354
BmiI GGNNCC 2 cut(s) 110, 350
BmrFI CCNGG 2 cut(s) 152, 231
BpuMI CCSGG 2 cut(s) 152, 231
BsaWI WCCGGW 2 cut(s) 264, 351
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 119, 345
Bse118I RCCGGY 1 cut(s) 351
BseAI TCCGGA 1 cut(s) 264
BseLI CCNNNNNNNGG 3 cut(s) 105, 119, 345
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 260
BseXI GCAGC 3 cut(s) 206, 221, 271
BseYI CCCAGC 1 cut(s) 68
Bsh1236I CGCG 5 cut(s) 41, 220, 227, 256, 302
Bsh1285I CGRYCG 3 cut(s) 218, 279, 321
BshFI GGCC 1 cut(s) 67
BshTI ACCGGT 1 cut(s) 351
BsiEI CGRYCG 3 cut(s) 218, 279, 321
BsiSI CCGG 5 cut(s) 54, 152, 231, 265, 352
BslI CCNNNNNNNGG 3 cut(s) 105, 119, 345
BsmAI GTCTC 1 cut(s) 281
BsmBI CGTCTC 1 cut(s) 281
BsnI GGCC 1 cut(s) 67
Bsp13I TCCGGA 1 cut(s) 264
Bsp143I GATC 6 cut(s) 114, 215, 267, 276, 318, 358
BspACI CCGC 6 cut(s) 39, 51, 81, 189, 220, 256
BspANI GGCC 1 cut(s) 67
BspCNI CTCAG 1 cut(s) 86
BspEI TCCGGA 1 cut(s) 264
BspFNI CGCG 5 cut(s) 41, 220, 227, 256, 302
BspHI TCATGA 1 cut(s) 295
BspLI GGNNCC 2 cut(s) 110, 350
BspPI GGATC 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 351
BssAI RCCGGY 1 cut(s) 351
BssMI GATC 6 cut(s) 114, 215, 267, 276, 318, 358
BstC8I GCNNGC 1 cut(s) 92
BstDEI CTNAG 1 cut(s) 73
BstFNI CGCG 5 cut(s) 41, 220, 227, 256, 302
BstHHI GCGC 4 cut(s) 43, 50, 229, 256
BstKTI GATC 6 cut(s) 117, 218, 270, 279, 321, 361
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 6 cut(s) 114, 215, 267, 276, 318, 358
BstMCI CGRYCG 3 cut(s) 218, 279, 321
BstMWI GCNNNNNNNGC 4 cut(s) 47, 78, 87, 226
BstSCI CCNGG 2 cut(s) 150, 229
BstUI CGCG 5 cut(s) 41, 220, 227, 256, 302
BstV1I GCAGC 3 cut(s) 206, 221, 271
BsuRI GGCC 1 cut(s) 67
BtgZI GCGATG 1 cut(s) 317
Cac8I GCNNGC 1 cut(s) 92
CciI TCATGA 1 cut(s) 295
CfoI GCGC 4 cut(s) 43, 50, 229, 256
Cfr10I RCCGGY 1 cut(s) 351
Cfr13I GGNCC 4 cut(s) 66, 108, 126, 354
CpoI CGGWCCG 1 cut(s) 354
CseI GACGC 1 cut(s) 308
CspAI ACCGGT 1 cut(s) 351
CspI CGGWCCG 1 cut(s) 354
CviAII CATG 1 cut(s) 296
CviJI RGCY 6 cut(s) 67, 72, 90, 197, 234, 262
CviKI_1 RGCY 6 cut(s) 67, 72, 90, 197, 234, 262
DdeI CTNAG 1 cut(s) 73
DpnI GATC 6 cut(s) 116, 217, 269, 278, 320, 360
DpnII GATC 6 cut(s) 114, 215, 267, 276, 318, 358
EciI GGCGGA 1 cut(s) 204
Eco47I GGWCC 3 cut(s) 108, 126, 354
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 1 cut(s) 299
FaiI YATR 2 cut(s) 297, 364
FatI CATG 1 cut(s) 295
FauI CCCGC 1 cut(s) 74
Fnu4HI GCNGC 5 cut(s) 51, 195, 235, 257, 260
Fsp4HI GCNGC 5 cut(s) 51, 195, 235, 257, 260
GlaI GCGC 4 cut(s) 42, 49, 228, 255
GluI GCNGC 5 cut(s) 51, 195, 235, 257, 260
GsaI CCCAGC 1 cut(s) 72
HaeIII GGCC 1 cut(s) 67
HapII CCGG 5 cut(s) 54, 152, 231, 265, 352
HgaI GACGC 1 cut(s) 308
HhaI GCGC 4 cut(s) 43, 50, 229, 256
Hin1II CATG 1 cut(s) 299
Hin6I GCGC 4 cut(s) 41, 48, 227, 254
HinP1I GCGC 4 cut(s) 41, 48, 227, 254
HinfI GANTC 3 cut(s) 167, 200, 330
HpaII CCGG 5 cut(s) 54, 152, 231, 265, 352
Hpy166II GTNNAC 2 cut(s) 6, 108
Hpy188I TCNGA 4 cut(s) 133, 172, 340, 358
Hpy188III TCNNGA 5 cut(s) 265, 271, 280, 296, 346
Hpy8I GTNNAC 2 cut(s) 6, 108
Hpy99I CGWCG 2 cut(s) 176, 319
HpyAV CCTTC 2 cut(s) 57, 244
HpyCH4IV ACGT 1 cut(s) 290
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 78, 87, 226
HpyF3I CTNAG 1 cut(s) 73
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 1 cut(s) 299
HspAI GCGC 4 cut(s) 41, 48, 227, 254
Kpn2I TCCGGA 1 cut(s) 264
Kzo9I GATC 6 cut(s) 114, 215, 267, 276, 318, 358
LmnI GCTCC 2 cut(s) 191, 267
LpnPI CCDG 6 cut(s) 67, 82, 165, 244, 278, 331
Lsp1109I GCAGC 3 cut(s) 206, 221, 271
MaeII ACGT 1 cut(s) 290
MalI GATC 6 cut(s) 116, 217, 269, 278, 320, 360
MboI GATC 6 cut(s) 114, 215, 267, 276, 318, 358
MboII GAAGA 1 cut(s) 109
MlyI GAGTC 3 cut(s) 176, 194, 324
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 6 cut(s) 139, 148, 178, 204, 238, 346
MroI TCCGGA 1 cut(s) 264
MspI CCGG 5 cut(s) 54, 152, 231, 265, 352
MspR9I CCNGG 2 cut(s) 152, 231
MvnI CGCG 5 cut(s) 41, 220, 227, 256, 302
MwoI GCNNNNNNNGC 4 cut(s) 47, 78, 87, 226
NciI CCSGG 2 cut(s) 152, 231
NdeII GATC 6 cut(s) 114, 215, 267, 276, 318, 358
NlaIII CATG 1 cut(s) 299
NlaIV GGNNCC 2 cut(s) 110, 350
PagI TCATGA 1 cut(s) 295
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PflFI GACNNNGTC 1 cut(s) 166
PflMI CCANNNNNTGG 1 cut(s) 105
PinAI ACCGGT 1 cut(s) 351
PkrI GCNGC 5 cut(s) 52, 196, 236, 258, 261
Ple19I CGATCG 3 cut(s) 218, 279, 321
PleI GAGTC 3 cut(s) 175, 194, 324
PpsI GAGTC 3 cut(s) 175, 194, 324
PspFI CCCAGC 1 cut(s) 68
PspN4I GGNNCC 2 cut(s) 110, 350
PspPI GGNCC 4 cut(s) 66, 108, 126, 354
PsyI GACNNNGTC 1 cut(s) 166
PvuI CGATCG 3 cut(s) 218, 279, 321
RgaI GCGATCGC 1 cut(s) 218
Rsr2I CGGWCCG 1 cut(s) 354
RsrII CGGWCCG 1 cut(s) 354
SatI GCNGC 5 cut(s) 51, 195, 235, 257, 260
Sau3AI GATC 6 cut(s) 114, 215, 267, 276, 318, 358
Sau96I GGNCC 4 cut(s) 66, 108, 126, 354
SchI GAGTC 3 cut(s) 176, 194, 324
ScrFI CCNGG 2 cut(s) 152, 231
SetI ASST 3 cut(s) 74, 264, 293
SfaAI GCGATCGC 1 cut(s) 218
SgfI GCGATCGC 1 cut(s) 218
SinI GGWCC 3 cut(s) 108, 126, 354
SsiI CCGC 6 cut(s) 39, 51, 81, 189, 220, 256
StyD4I CCNGG 2 cut(s) 150, 229
TaiI ACGT 1 cut(s) 293
TaqI TCGA 3 cut(s) 203, 279, 317
TauI GCSGC 2 cut(s) 53, 259
TseI GCWGC 3 cut(s) 194, 234, 259
TspGWI ACGGA 3 cut(s) 14, 179, 257
Tth111I GACNNNGTC 1 cut(s) 166
Van91I CCANNNNNTGG 1 cut(s) 105
VpaK11BI GGWCC 3 cut(s) 108, 126, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.