Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010443

Physical Location & Seq

Genomic Coordinates Forward (+)

3920826 .. 3925070

Gene Structure 4245 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1050300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4155 bp

ATGGAGAAAGATCCGATCAGTGAAGAAAACGGCAAATCGAGCCGGAACAAGCAAACAAAATTCAAGAGGAAAGAGCTGGGATTGTCGTGCATGCTAAACACCGAACTCGGCGCCGTCCTTGCCGTCATCCGCCGTCCCCTCAACCCGACTTCCCACTGCTTCGCCGCCCCGGAAGACACCATCGACCCTTCCCTTCTCCAATCCCTAAAATCCCTCCGCGCCCTGATCTTCAATCCCCAGCAGGAATGGCGCACAATAGACCCCTCCATCTACCTCTCCCCCTTCCTGGATGTCATCCAAAGCGAGGACGTCCACGCCGCTGCCACTCGCGTCGCCCTCTCCGCCACGCTGAAGATTATCAAGCTCGGAGTCTTCGACGAGAAGACTCCGGGGGCGAAAGAAGCTATTAACACCATGGTCACATCCATCACCACGTGCCGGCTCGAAAGGACCGATTCTGTCAACGAGGACGCCGTGATGCTGAGCATCCTTCAGGTCCTGACCGGAATCATGAACCACCGAGCTTCGGTTCTGCTCTCCGATCAGTCAGTGTGCACAATTGTGAACACCTGTTTTCAGGTGGTTCAGCAATCGGCGAACAGAGGAGACTTGCTAATGCGAAGTGCGAGGTACACAATGCACGAGCTGATCCAGATCATTTTTTCGCGGTTGCCGGAAATTGATTTCAGGGATGACGAGAGCTTGGCCAGTACTGACACCGAGGACGCCGATGCAGACGATGGGACTATGGATTCCGGATATGGGATCCGGTGTGCGGTTGATATTTTTCACTTCTTGTGTTCTTTGTTGAATGTGGTTCAGGTGGTTGAGACAGATCAAGGGTCTACTGTGCAAACTGCCAACGAGGATGTGCAGCTTTTCGCTCTCGTTCTGATCAACTCCGCGATCGATTTGAGCGGGGATGGAATCGGGTCGCATTCGAAGTTGTTGAGGATGATGAAAGACGATCTCTTTCACCATTTGGTTCACTACGGCACGCGTTCAAGCCCGCTTGTTTTTTCCATGATTTGCAGTACGGTTTTGAACATGTATCACTTTCTTCGGAGGTTCATTCGTCTTCAGTTAGAAGCGTTCTTCACGTTTGTGCTGTTTCGAGTTTCAGCTCCCGGAGTGTCAATCCAACTTCAAGAGGTTGCACTTGAAGGGATTATAAATTTCTGCAGACAACTGACATTCGTCGTAGAGGTCTATGTGAACTACGACTGTGATCCGCTTTGCCATAATGTGTTCGAGGAGATAGGGAAGTTGCTTTGCAAGCTGTCCTTTCCGGTCGCAAGCCCTTTGACAACTTTACAGATACAAGCCTTTGAAGGGTTAGTTATCATGATCCACAACATTGCAGACAGTATTGATAAAGAAAATGATACAAGCCCGTCTGGACCCTACCCGATTGAGATCAGAGAGTATACGCCGTTTTGGGAAGACAAACCAAAAGATGATTCAGAAGCTTGGGTGCAATTTGTAAGGCTGAGGAAAGCGCAGAAGAGGAAAATATTGATTGCCGGACACCATTTTAATTGCGACGAAAAGAAGGGAATGGAGTACTTGAAGCTTTCCAACTTAGTCTCTGACCCTCCGGATCCAAAGACTTTGGCTTATTTCTTTCGTTACACTCCTGGGCTTGACAAGACGATGATAGGCGATTATCTTGGTGATCCTGATGAGTTTCACATCAAAGTACTTAAAGAATTCACGGGAGCCTTTGGATTTTCAGGCATGAATCTCGATAGTGCTCTCCGAACTTATCTGGAGACTTTCCGTTTGCCAGGAGAGTCCCAGAAGATTCAGAGGATTCTCGAAGCGTTTTCAGAGAGTTTTTTTGAGCAGCAGTCTGCAGATGTTTTTGTGAACAAAGATACTGTATTCATTCTTTGCTACTCTCTCATTATGCTCAATACTGACCAGCACAATCCGCAAGTAAAGAAGAAGATGACGGAAGAAGAGTTCATCAGGAATAATAGGGCAATCAATGGAGGGAAGGATCTTCCTAGAGAGTATCTGTCTGAGCTTTTCCAATCCATATCGAACAATGCAATCACGCTTTTTGGTCAATCTGGTATGACAGTACTAATGAACCCGAGCAGATGGATTGAGTTGATGAACTGTTCCAAAACAGTGCAGCCTTTCATTCTGTGTGATTTTGATCGTCGGCTAGGAAGAGACATGTTTGCTTGCATCGCTGGCCCTTCAGTCGCTACTATTTCTGCATTTTTCGAGCAAGCTGAAGAAGAGGAGTTGCTCCATGAATGCATTGAAGGATTATTCTCGGTAGCCAGGATAGCGCAGTATGGACTGGAGGACACACTCGATGAGCTCATTGCCACCTTCTCGAGATTCACCACGCTGCTTAATCCTTACGCCTCTGCAGAAGAAACACTCTTTGCTTTTAGCAAAGATTTGAAGCCAAGAATGGCTACACTCGCTGTTTTCACCATCGCAAACAACTTTGGAGAGTCAATCAGAGGGGGTTGGAGGAACATTGTGGACTGTTTGTTGAAACTCAAGAGGCTTAAACTCCTTCCCCAAAATGTTCTCGAATTTGATGCTTCCACCTCATCTGATGCTCAACCTACATCTGAATCGGGTGTAATCTTCCCTGGTCACGATCCCAAATTTGGAGGACGACAGGCTTCTGGCATGATTAGTCGATTCTCACATTTTCTATCACTAGAGAGTCCGGAAGATGCTGTATCTCTTGGCATGAGTGAATTTGAACAGAACCTGAAGGTTATCAAACAATGCCGAATTGGGAACATCTTCAGCACTAGTTCAAACTTTCCTGAGGACTCTTTGCTGAACCTCGGGCGTTCGCTAATATATGCAGCTGCAGGGAAAGGGCAGAAGTTTAGCACACCTGTTGAAGAAGAAGAGGCCGTTGAGTTCTGCTGGGATTTGATGGTTGCCATCTCTTTGGCCAACGTCCATAGATTCCAAGCATTTTGGTCTAGTTTTAATGATTATCTGCTGGCGGTTGCTCAGTTTCCGATGTTCTCCCCAATCCCATTTGCTGAAAAGGCCATTGTAGGCCTCTTCAAGGTCTGTCTGAAGCTCCTTGGCACATACCAACCTGACAGAGTCCCCGAGGAGCTCATTTTCAAGTCGATAAATCTAATGTGGAAGCTGGAAAAAGAAATTCTCGACACTTGCAGCGAGTCCATTACACAATCATTAAACAAGATTCTCGTTGAGTACCCTGCCAATTTGCAAACCCAACTCGGATGGAAGTCAGTTCTCCACTTGTTATCAGTCTCCGGGCGACACCCTGACACCTATGAGCAGGGAGTTGAGACTCTGATCATGGTGATGTCCGATGGAACCCATGTATCGCGGACAAATTATGCTTACTTCATTGACTGTGCCTTTGGTTTCATTGCTCTAAAAAACAGCCCATCAGAGAAGAACTTGAAGATACTTGACCTATTAGCGGATTCTGTAAATTTGTTGATCCAATGGTACAGAAATCATTACTCAGATCCCGGGAACAATTACAGCATCGCGAGCAACACTAGTACCTCCTCCTTAGAAGACTCGAAAGGTTTCGGCTCTAACAACTTTACCATGAATTTGTTTGTCAAGCTAGGGGAGGCATTCCGAAAGACCAGCCTAGCTCGGCGAGAAGAAATAAGAAACCATGCAGTCTTGTCTCTTAAAAGAAGTTTCCAACTTGCTGAGGAACTAGAATTCACACCAACCAACTGTATCAACTGTTTCAACCTCCTTATCTTCGCGATGGTGGATGATCTGCACGAAAAGATGCTGGAGTACTCACGAAGAGAGAACGCAGAGAAGGAGATGAGAGGCATGGAAGGGACCTTGAAGATTGCATTGGAGCTCCTCACAGATGTGTACTTGCAGTTTCTGATACCAATTTCGCAATGCAGCGGCTTTCGGACATTTTGGTTAGGAGTGTTGAGAAGGATGGACACATGTATGAAGGCGGATTTGGGATCGTATGGTGAATCAAAACTGCCGGAAGTTGTGCCGGAGTTGCTGAGAAAGTTGATTACGGAGATGAAGGAGAAGGAGATTTTAGTGCCGAAGGAGGGTGACGATCTTTGGGAGATAACACACATTCAGATACAGTGGATAGCTCCTTCAATCAAGGAGGAGCTATTTCCAGAAGAAAGTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0005085 GO:0005086 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0008150 GO:0009846 GO:0009856 GO:0012505 GO:0016020 GO:0016192 GO:0019899 GO:0022414 GO:0031984 GO:0032501 GO:0042995 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0051020 GO:0051179 GO:0051234 GO:0051704 GO:0065007 GO:0065009 GO:0090406 GO:0098772 GO:0098791 GO:0120025

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

DCB Sec7 Sec7_N

Protein Analysis

1385

Amino Acids

156.43

Weight (kDa)

5.14

Isoelectric Point (pI)

45.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec7-like_HUS	PF12783	256 - 416	2.2e-36	Mon2/Sec7/BIG1-like, HUS domain
Sec7	PF01369	501 - 686	5.6e-70	Sec7 domain
TPR_28	PF23325	1207 - 1366	1.2e-06	GBF1-like, tetratrico peptide repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010443)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19610
fragaria_vesca	FvH4_1g05140
malus_domestica	MD02G1050300.v1.1 MD15G1187600.v1.1
prunus_persica	Prupe.7G228800_v2.0.a1
pyrus_communis	pycom02g04190 pycom02g04200 pycom15g16840
rosa_chinensis	RchiOBHm_Chr2g0090771 RchiOBHm_Chr2g0090781
rosa_laevigata	RLG00000016142
rosa_multiflora	Rmu_co8351113.1_g000001 Rmu_ssc0000367.1_g000008
rosa_roxburghii	Rroxscaffold_2G00150830
rosa_rugosa	Rorug02G0010400
rosa_samantha	Rh2AG056400 Rh2BG055100 Rh2CG057500 Rh2DG056000 Rh2DG056100
rosa_wichuraiana	Rw2G004610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1172
AatII	GACGTC	1 cut(s)	312
AccB1I	GGYRCC	1 cut(s)	110
AccBSI	CCGCTC	1 cut(s)	918
AccI	GTMKAC	2 cut(s)	845, 1427
AccII	CGCG	8 cut(s)	219, 330, 667, 905, 1000, 3349, 3518, 3749
AccIII	TCCGGA	3 cut(s)	755, 1597, 2698
AcoI	YGGCCR	2 cut(s)	705, 2934
AcuI	CTGAAG	8 cut(s)	371, 476, 1064, 2193, 2265, 2764, 2765, 3086
AcvI	CACGTG	1 cut(s)	435
AcyI	GRCGYC	4 cut(s)	111, 309, 471, 726
AdeI	CACNNNGTG	1 cut(s)	435
AflIII	ACRYGT	4 cut(s)	998, 1047, 2184, 3947
AhlI	ACTAGT	2 cut(s)	2786, 3527
AjnI	CCWGG	5 cut(s)	285, 1636, 1786, 2294, 2617
AjuI	GAANNNNNNNTTGG	6 cut(s)	1444, 1476, 3830, 3862, 3947, 3979
AleI	CACNNNNGTG	3 cut(s)	560, 1103, 3863
AloI	GAACNNNNNNTCC	2 cut(s)	1949, 1981
Alw21I	GWGCWC	5 cut(s)	557, 1756, 2337, 3111, 3855
Alw26I	GTCTC	8 cut(s)	600, 824, 1591, 1766, 2175, 3274, 3302, 3669
Alw44I	GTGCAC	1 cut(s)	553
AlwNI	CAGNNNCTG	3 cut(s)	499, 2743, 3880
Ama87I	CYCGRG	5 cut(s)	2098, 2350, 2822, 3101, 3497
Aor13HI	TCCGGA	3 cut(s)	755, 1597, 2698
AoxI	GGCC	6 cut(s)	705, 2203, 2892, 2934, 3036, 3046
ApaLI	GTGCAC	1 cut(s)	553
ApeKI	GCWGC	9 cut(s)	320, 874, 1846, 2140, 2365, 2843, 2846, 3168, 3900
ArsI	GACNNNNNNTTYG	4 cut(s)	1765, 1797, 3365, 3397
Asp700I	GAANNNNTTC	2 cut(s)	2777, 3557
AspLEI	GCGC	5 cut(s)	113, 221, 252, 1501, 2305
AspS9I	GGNCC	5 cut(s)	450, 496, 1400, 2204, 3831
AsuC2I	CCSGG	6 cut(s)	170, 390, 1128, 3274, 3498, 3499
AsuHPI	GGTGA	8 cut(s)	421, 968, 1685, 2350, 2443, 3334, 3989, 4079
AsuII	TTCGAA	1 cut(s)	941
AvaI	CYCGRG	5 cut(s)	2098, 2350, 2822, 3101, 3497
AvaII	GGWCC	4 cut(s)	450, 496, 1400, 3831
AxyI	CCTNAGG	1 cut(s)	2802
BaeGI	GKGCMC	1 cut(s)	557
BaeI	ACNNNNGTAYC	4 cut(s)	3327, 3360, 3514, 3547
BalI	TGGCCA	2 cut(s)	707, 2936
BamHI	GGATCC	2 cut(s)	765, 1600
BanI	GGYRCC	1 cut(s)	110
BanII	GRGCYC	3 cut(s)	2337, 3111, 3855
BauI	CACGAG	1 cut(s)	641
BbrPI	CACGTG	1 cut(s)	435
BbsI	GAAGAC	6 cut(s)	180, 364, 389, 1070, 1449, 3552
Bbv12I	GWGCWC	5 cut(s)	557, 1756, 2337, 3111, 3855
BbvCI	CCTCAGC	2 cut(s)	1490, 3690
BbvI	GCAGC	9 cut(s)	307, 886, 1858, 2152, 2352, 2833, 2855, 3180, 3912
BceAI	ACGGC	8 cut(s)	46, 98, 107, 117, 458, 1009, 1417, 2879
BcgI	CGANNNNNNTGC	4 cut(s)	1726, 1760, 2546, 2580
BciT130I	CCWGG	5 cut(s)	287, 1638, 1788, 2296, 2619
BclI	TGATCA	2 cut(s)	894, 3315
BcnI	CCSGG	6 cut(s)	170, 390, 1128, 3274, 3498, 3499
BcoDI	GTCTC	8 cut(s)	600, 824, 1591, 1766, 2175, 3274, 3302, 3669
BcuI	ACTAGT	2 cut(s)	2786, 3527
BfaI	CTAG	9 cut(s)	2010, 2174, 2690, 2787, 2967, 3528, 3599, 3626, 3698
BfmI	CTRYAG	4 cut(s)	1180, 1854, 2385, 2847
BfoI	RGCGCY	1 cut(s)	114
BlpI	GCTNAGC	1 cut(s)	482
BmcAI	AGTACT	5 cut(s)	712, 1565, 1701, 2088, 3785
Bme18I	GGWCC	4 cut(s)	450, 496, 1400, 3831
BmeT110I	CYCGRG	5 cut(s)	2098, 2350, 2822, 3101, 3497
BmgT120I	GGNCC	5 cut(s)	450, 496, 1400, 2204, 3831
BmiI	GGNNCC	7 cut(s)	112, 767, 1402, 1602, 1720, 3337, 3832
BmsI	GCATC	9 cut(s)	468, 495, 721, 2205, 2554, 2572, 2695, 3522, 3765
BoxI	GACNNNNGTC	1 cut(s)	1196
BpiI	GAAGAC	6 cut(s)	180, 364, 389, 1070, 1449, 3552
BpmI	CTGGAG	3 cut(s)	1790, 2336, 3800
Bpu10I	CCTNAGC	2 cut(s)	1490, 3690
Bpu1102I	GCTNAGC	1 cut(s)	482
Bpu14I	TTCGAA	1 cut(s)	941
BpuEI	CTTGAG	1 cut(s)	2507
BpuMI	CCSGG	6 cut(s)	170, 390, 1128, 3274, 3498, 3499
Bsa29I	ATCGAT	1 cut(s)	909
BsaAI	YACGTR	1 cut(s)	435
BsaBI	GATNNNNATC	3 cut(s)	653, 1415, 2163
BsaHI	GRCGYC	4 cut(s)	111, 309, 471, 726
BsaWI	WCCGGW	6 cut(s)	503, 755, 768, 1288, 1597, 2698
Bse118I	RCCGGY	1 cut(s)	438
Bse1I	ACTGG	2 cut(s)	708, 2319
Bse21I	CCTNAGG	1 cut(s)	2802
Bse3DI	GCAATG	4 cut(s)	1356, 2337, 3390, 3902
Bse8I	GATNNNNATC	3 cut(s)	653, 1415, 2163
BseAI	TCCGGA	3 cut(s)	755, 1597, 2698
BseBI	CCWGG	5 cut(s)	287, 1638, 1788, 2296, 2619
BseCI	ATCGAT	1 cut(s)	909
BseJI	GATNNNNATC	3 cut(s)	653, 1415, 2163
BseMI	GCAATG	4 cut(s)	1356, 2337, 3390, 3902
BseMII	CTCAG	8 cut(s)	473, 1481, 2016, 2793, 3011, 3504, 3681, 4004
BseNI	ACTGG	2 cut(s)	708, 2319
BseRI	GAGGAG	7 cut(s)	618, 1268, 2267, 3119, 3526, 3845, 4142
BseSI	GKGCMC	1 cut(s)	557
BseXI	GCAGC	9 cut(s)	307, 886, 1858, 2152, 2352, 2833, 2855, 3180, 3912
BseYI	CCCAGC	3 cut(s)	76, 237, 2907
BsgI	GTGCAG	3 cut(s)	893, 2159, 3749
Bsh1236I	CGCG	8 cut(s)	219, 330, 667, 905, 1000, 3349, 3518, 3749
Bsh1285I	CGRYCG	2 cut(s)	909, 1293
BshFI	GGCC	6 cut(s)	707, 2205, 2894, 2936, 3038, 3048
BshNI	GGYRCC	1 cut(s)	110
BshVI	ATCGAT	1 cut(s)	909
BsiEI	CGRYCG	2 cut(s)	909, 1293
BsiHKAI	GWGCWC	5 cut(s)	557, 1756, 2337, 3111, 3855
BsiHKCI	CYCGRG	5 cut(s)	2098, 2350, 2822, 3101, 3497
BslFI	GGGAC	5 cut(s)	120, 757, 1780, 3083, 3844
BsmAI	GTCTC	8 cut(s)	600, 824, 1591, 1766, 2175, 3274, 3302, 3669
BsmFI	GGGAC	5 cut(s)	120, 757, 1780, 3083, 3844
BsmI	GAATGC	3 cut(s)	937, 2273, 3608
BsnI	GGCC	6 cut(s)	707, 2205, 2894, 2936, 3038, 3048
BsoBI	CYCGRG	5 cut(s)	2098, 2350, 2822, 3101, 3497
Bsp119I	TTCGAA	1 cut(s)	941
Bsp1286I	GDGCHC	5 cut(s)	557, 1756, 2337, 3111, 3855
Bsp13I	TCCGGA	3 cut(s)	755, 1597, 2698
Bsp1720I	GCTNAGC	1 cut(s)	482
Bsp19I	CCATGG	1 cut(s)	414
Bsp68I	TCGCGA	2 cut(s)	3518, 3749
BspANI	GGCC	6 cut(s)	707, 2205, 2894, 2936, 3038, 3048
BspCNI	CTCAG	8 cut(s)	474, 1482, 2017, 2794, 3010, 3503, 3682, 4005
BspDI	ATCGAT	1 cut(s)	909
BspEI	TCCGGA	3 cut(s)	755, 1597, 2698
BspFNI	CGCG	8 cut(s)	219, 330, 667, 905, 1000, 3349, 3518, 3749
BspHI	TCATGA	2 cut(s)	510, 1344
BspLI	GGNNCC	7 cut(s)	112, 767, 1402, 1602, 1720, 3337, 3832
BspMAI	CTGCAG	4 cut(s)	1184, 1858, 2389, 2851
BspT104I	TTCGAA	1 cut(s)	941
BspT107I	GGYRCC	1 cut(s)	110
BsrBI	CCGCTC	1 cut(s)	918
BsrDI	GCAATG	4 cut(s)	1356, 2337, 3390, 3902
BsrFI	RCCGGY	1 cut(s)	438
BsrI	ACTGG	2 cut(s)	708, 2319
BssAI	RCCGGY	1 cut(s)	438
BssNAI	GTATAC	1 cut(s)	1428
BssNI	GRCGYC	4 cut(s)	111, 309, 471, 726
BssSI	CACGAG	1 cut(s)	641
BssT1I	CCWWGG	2 cut(s)	414, 3073
Bst1107I	GTATAC	1 cut(s)	1428
Bst2BI	CACGAG	1 cut(s)	641
Bst2UI	CCWGG	5 cut(s)	287, 1638, 1788, 2296, 2619
Bst6I	CTCTTC	7 cut(s)	1499, 1956, 2173, 2244, 2883, 3056, 3787
BstACI	GRCGYC	4 cut(s)	111, 309, 471, 726
BstBAI	YACGTR	1 cut(s)	435
BstBI	TTCGAA	1 cut(s)	941
BstDSI	CCRYGG	1 cut(s)	414
BstENI	CCTNNNNNAGG	1 cut(s)	3053
BstFNI	CGCG	8 cut(s)	219, 330, 667, 905, 1000, 3349, 3518, 3749
BstH2I	RGCGCY	1 cut(s)	114
BstHHI	GCGC	5 cut(s)	113, 221, 252, 1501, 2305
BstMAI	GTCTC	8 cut(s)	600, 824, 1591, 1766, 2175, 3274, 3302, 3669
BstMCI	CGRYCG	2 cut(s)	909, 1293
BstNI	CCWGG	5 cut(s)	287, 1638, 1788, 2296, 2619
BstNSI	RCATGY	4 cut(s)	94, 1051, 2188, 3951
BstPAI	GACNNNNGTC	1 cut(s)	1196
BstSFI	CTRYAG	4 cut(s)	1180, 1854, 2385, 2847
BstSLI	GKGCMC	1 cut(s)	557
BstUI	CGCG	8 cut(s)	219, 330, 667, 905, 1000, 3349, 3518, 3749
BstV1I	GCAGC	9 cut(s)	307, 886, 1858, 2152, 2352, 2833, 2855, 3180, 3912
BstV2I	GAAGAC	6 cut(s)	180, 364, 389, 1070, 1449, 3552
BstX2I	RGATCY	5 cut(s)	10, 765, 1600, 2002, 3493
BstXI	CCANNNNNNTGG	1 cut(s)	2932
BstYI	RGATCY	5 cut(s)	10, 765, 1600, 2002, 3493
BstZ17I	GTATAC	1 cut(s)	1428
Bsu15I	ATCGAT	1 cut(s)	909
Bsu36I	CCTNAGG	1 cut(s)	2802
BsuRI	GGCC	6 cut(s)	707, 2205, 2894, 2936, 3038, 3048
BsuTUI	ATCGAT	1 cut(s)	909
BtgI	CCRYGG	1 cut(s)	414
BtgZI	GCGATG	4 cut(s)	2182, 2440, 3499, 3764
BtsI	GCAGTG	1 cut(s)	154
BtsIMutI	CAGTG	5 cut(s)	25, 154, 555, 2142, 4109
BtuMI	TCGCGA	2 cut(s)	3518, 3749
CaiI	CAGNNNCTG	3 cut(s)	499, 2743, 3880
CciI	TCATGA	2 cut(s)	510, 1344
CfoI	GCGC	5 cut(s)	113, 221, 252, 1501, 2305
Cfr10I	RCCGGY	1 cut(s)	438
Cfr13I	GGNCC	5 cut(s)	450, 496, 1400, 2204, 3831
Cfr9I	CCCGGG	1 cut(s)	3497
ClaI	ATCGAT	1 cut(s)	909
CseI	GACGC	3 cut(s)	319, 479, 734
DinI	GGCGCC	1 cut(s)	112
DraIII	CACNNNGTG	1 cut(s)	435
EaeI	YGGCCR	2 cut(s)	705, 2934
Eam1104I	CTCTTC	7 cut(s)	1499, 1956, 2173, 2244, 2883, 3056, 3787
EarI	CTCTTC	7 cut(s)	1499, 1956, 2173, 2244, 2883, 3056, 3787
EciI	GGCGGA	3 cut(s)	119, 331, 3974
Ecl136II	GAGCTC	3 cut(s)	2335, 3109, 3853
Eco130I	CCWWGG	2 cut(s)	414, 3073
Eco147I	AGGCCT	1 cut(s)	3048
Eco24I	GRGCYC	3 cut(s)	2337, 3111, 3855
Eco47I	GGWCC	4 cut(s)	450, 496, 1400, 3831
Eco53kI	GAGCTC	3 cut(s)	2335, 3109, 3853
Eco57I	CTGAAG	8 cut(s)	371, 476, 1064, 2193, 2265, 2764, 2765, 3086
Eco72I	CACGTG	1 cut(s)	435
Eco81I	CCTNAGG	1 cut(s)	2802
Eco88I	CYCGRG	5 cut(s)	2098, 2350, 2822, 3101, 3497
EcoICRI	GAGCTC	3 cut(s)	2335, 3109, 3853
EcoNI	CCTNNNNNAGG	1 cut(s)	3053
EcoO109I	RGGNCCY	2 cut(s)	496, 3831
EcoRI	GAATTC	2 cut(s)	1709, 3701
EcoRII	CCWGG	5 cut(s)	285, 1636, 1786, 2294, 2617
EcoT14I	CCWWGG	2 cut(s)	414, 3073
EcoT22I	ATGCAT	1 cut(s)	2273
EcoT38I	GRGCYC	3 cut(s)	2337, 3111, 3855
EgeI	GGCGCC	1 cut(s)	112
EheI	GGCGCC	1 cut(s)	112
ErhI	CCWWGG	2 cut(s)	414, 3073
FalI	AAGNNNNNCTT	2 cut(s)	1268, 1300
FaqI	GGGAC	5 cut(s)	120, 757, 1780, 3083, 3844
FauI	CCCGC	2 cut(s)	911, 1017
FbaI	TGATCA	2 cut(s)	894, 3315
FblI	GTMKAC	2 cut(s)	845, 1427
FriOI	GRGCYC	3 cut(s)	2337, 3111, 3855
FspBI	CTAG	9 cut(s)	2010, 2174, 2690, 2787, 2967, 3528, 3599, 3626, 3698
GlaI	GCGC	5 cut(s)	112, 220, 251, 1500, 2304
GsaI	CCCAGC	3 cut(s)	80, 241, 2911
GsuI	CTGGAG	3 cut(s)	1790, 2336, 3800
HaeII	RGCGCY	1 cut(s)	114
HaeIII	GGCC	6 cut(s)	707, 2205, 2894, 2936, 3038, 3048
HgaI	GACGC	3 cut(s)	319, 479, 734
HhaI	GCGC	5 cut(s)	113, 221, 252, 1501, 2305
Hin1I	GRCGYC	4 cut(s)	111, 309, 471, 726
Hin6I	GCGC	5 cut(s)	111, 219, 250, 1499, 2303
HinP1I	GCGC	5 cut(s)	111, 219, 250, 1499, 2303
HincII	GTYRAC	1 cut(s)	463
HindII	GTYRAC	1 cut(s)	463
HindIII	AAGCTT	2 cut(s)	1467, 1571
HphI	GGTGA	8 cut(s)	421, 968, 1685, 2350, 2443, 3334, 3989, 4079
Hpy99I	CGWCG	5 cut(s)	335, 380, 1202, 1547, 2172
HpyCH4IV	ACGT	4 cut(s)	309, 434, 1100, 2940
HpySE526I	ACGT	4 cut(s)	309, 434, 1100, 2940
Hsp92I	GRCGYC	4 cut(s)	111, 309, 471, 726
HspAI	GCGC	5 cut(s)	111, 219, 250, 1499, 2303
KasI	GGCGCC	1 cut(s)	110
Kpn2I	TCCGGA	3 cut(s)	755, 1597, 2698
KroI	GCCGGC	1 cut(s)	438
KroNI	GCCGGC	1 cut(s)	440
Ksp22I	TGATCA	2 cut(s)	894, 3315
LmnI	GCTCC	9 cut(s)	1129, 1718, 2265, 3075, 3106, 3850, 3858, 4117, 4129
Lsp1109I	GCAGC	9 cut(s)	307, 886, 1858, 2152, 2352, 2833, 2855, 3180, 3912
LweI	GCATC	9 cut(s)	468, 495, 721, 2205, 2554, 2572, 2695, 3522, 3765
MaeI	CTAG	9 cut(s)	2010, 2174, 2690, 2787, 2967, 3528, 3599, 3626, 3698
MaeII	ACGT	4 cut(s)	309, 434, 1100, 2940
MaeIII	GTNAC	4 cut(s)	418, 1628, 2621, 4067
MbiI	CCGCTC	1 cut(s)	918
MfeI	CAATTG	1 cut(s)	558
MflI	RGATCY	5 cut(s)	10, 765, 1600, 2002, 3493
MhlI	GDGCHC	5 cut(s)	557, 1756, 2337, 3111, 3855
MlsI	TGGCCA	2 cut(s)	707, 2936
MluI	ACGCGT	1 cut(s)	998
MluNI	TGGCCA	2 cut(s)	707, 2936
Mly113I	GGCGCC	1 cut(s)	111
MmeI	TCCRAC	4 cut(s)	1165, 1602, 2471, 3706
Mox20I	TGGCCA	2 cut(s)	707, 2936
Mph1103I	ATGCAT	1 cut(s)	2273
MroI	TCCGGA	3 cut(s)	755, 1597, 2698
MroNI	GCCGGC	1 cut(s)	438
MroXI	GAANNNNTTC	2 cut(s)	2777, 3557
MscI	TGGCCA	2 cut(s)	707, 2936
MseI	TTAA	8 cut(s)	408, 1536, 1704, 2370, 2532, 2973, 3191, 3669
MslI	CAYNNNNRTG	5 cut(s)	560, 1103, 3323, 3863, 3950
Msp20I	TGGCCA	2 cut(s)	707, 2936
MspA1I	CMGCKG	3 cut(s)	320, 2846, 3903
MunI	CAATTG	1 cut(s)	558
Mva1269I	GAATGC	3 cut(s)	937, 2273, 3608
MvaI	CCWGG	5 cut(s)	287, 1638, 1788, 2296, 2619
MvnI	CGCG	8 cut(s)	219, 330, 667, 905, 1000, 3349, 3518, 3749
NaeI	GCCGGC	1 cut(s)	440
NarI	GGCGCC	1 cut(s)	111
NciI	CCSGG	6 cut(s)	170, 390, 1128, 3274, 3498, 3499
NcoI	CCATGG	1 cut(s)	414
NgoMIV	GCCGGC	1 cut(s)	438
NlaIV	GGNNCC	7 cut(s)	112, 767, 1402, 1602, 1720, 3337, 3832
NmeAIII	GCCGAG	2 cut(s)	87, 3610
NmuCI	GTSAC	3 cut(s)	418, 2621, 4067
NruI	TCGCGA	2 cut(s)	3518, 3749
NsiI	ATGCAT	1 cut(s)	2273
NspI	RCATGY	4 cut(s)	94, 1051, 2188, 3951
NspV	TTCGAA	1 cut(s)	941
OliI	CACNNNNGTG	3 cut(s)	560, 1103, 3863
PaeI	GCATGC	1 cut(s)	94
PaeR7I	CTCGAG	1 cut(s)	2350
PagI	TCATGA	2 cut(s)	510, 1344
PceI	AGGCCT	1 cut(s)	3048
PciI	ACATGT	3 cut(s)	1047, 2184, 3947
PcsI	WCGNNNNNNNCGW	3 cut(s)	372, 450, 471
PctI	GAATGC	3 cut(s)	937, 2273, 3608
PdiI	GCCGGC	1 cut(s)	440
PdmI	GAANNNNTTC	2 cut(s)	2777, 3557
PflFI	GACNNNGTC	1 cut(s)	3095
PfoI	TCCNGGA	2 cut(s)	285, 1126
Ple19I	CGATCG	1 cut(s)	909
PluTI	GGCGCC	1 cut(s)	114
PmaCI	CACGTG	1 cut(s)	435
PmlI	CACGTG	1 cut(s)	435
Ppu21I	YACGTR	1 cut(s)	435
PpuMI	RGGWCCY	2 cut(s)	496, 3831
PscI	ACATGT	3 cut(s)	1047, 2184, 3947
PshAI	GACNNNNGTC	1 cut(s)	1196
PsiI	TTATAA	1 cut(s)	1172
Psp124BI	GAGCTC	3 cut(s)	2337, 3111, 3855
Psp5II	RGGWCCY	2 cut(s)	496, 3831
Psp6I	CCWGG	5 cut(s)	285, 1636, 1786, 2294, 2617
PspCI	CACGTG	1 cut(s)	435
PspFI	CCCAGC	3 cut(s)	76, 237, 2907
PspGI	CCWGG	5 cut(s)	285, 1636, 1786, 2294, 2617
PspN4I	GGNNCC	7 cut(s)	112, 767, 1402, 1602, 1720, 3337, 3832
PspPI	GGNCC	5 cut(s)	450, 496, 1400, 2204, 3831
PspPPI	RGGWCCY	2 cut(s)	496, 3831
PstI	CTGCAG	4 cut(s)	1184, 1858, 2389, 2851
PstNI	CAGNNNCTG	3 cut(s)	499, 2743, 3880
PsuI	RGATCY	5 cut(s)	10, 765, 1600, 2002, 3493
PsyI	GACNNNGTC	1 cut(s)	3095
PvuI	CGATCG	1 cut(s)	909
PvuII	CAGCTG	1 cut(s)	2846
RruI	TCGCGA	2 cut(s)	3518, 3749
RseI	CAYNNNNRTG	5 cut(s)	560, 1103, 3323, 3863, 3950
SacI	GAGCTC	3 cut(s)	2337, 3111, 3855
SaqAI	TTAA	8 cut(s)	408, 1536, 1704, 2370, 2532, 2973, 3191, 3669
Sau96I	GGNCC	5 cut(s)	450, 496, 1400, 2204, 3831
ScaI	AGTACT	5 cut(s)	712, 1565, 1701, 2088, 3785
SduI	GDGCHC	5 cut(s)	557, 1756, 2337, 3111, 3855
SfaNI	GCATC	9 cut(s)	468, 495, 721, 2205, 2554, 2572, 2695, 3522, 3765
SfcI	CTRYAG	4 cut(s)	1180, 1854, 2385, 2847
SfoI	GGCGCC	1 cut(s)	112
Sfr274I	CTCGAG	1 cut(s)	2350
SfuI	TTCGAA	1 cut(s)	941
SinI	GGWCC	4 cut(s)	450, 496, 1400, 3831
SlaI	CTCGAG	1 cut(s)	2350
SmaI	CCCGGG	1 cut(s)	3499
SmiMI	CAYNNNNRTG	5 cut(s)	560, 1103, 3323, 3863, 3950
SmlI	CTYRAG	2 cut(s)	2350, 2522
SmoI	CTYRAG	2 cut(s)	2350, 2522
SpeI	ACTAGT	2 cut(s)	2786, 3527
SphI	GCATGC	1 cut(s)	94
SseBI	AGGCCT	1 cut(s)	3048
SspDI	GGCGCC	1 cut(s)	110
SspI	AATATT	1 cut(s)	1515
SspMI	CTAG	9 cut(s)	2010, 2174, 2690, 2787, 2967, 3528, 3599, 3626, 3698
SstI	GAGCTC	3 cut(s)	2337, 3111, 3855
StuI	AGGCCT	1 cut(s)	3048
StyI	CCWWGG	2 cut(s)	414, 3073
TaiI	ACGT	4 cut(s)	312, 437, 1103, 2943
TaqII	GACCGA	1 cut(s)	467
TatI	WGTACW	6 cut(s)	710, 1563, 1699, 2086, 3783, 3867
TauI	GCSGC	3 cut(s)	167, 320, 3906
Tru1I	TTAA	8 cut(s)	408, 1536, 1704, 2370, 2532, 2973, 3191, 3669
Tru9I	TTAA	8 cut(s)	408, 1536, 1704, 2370, 2532, 2973, 3191, 3669
TscAI	CASTG	5 cut(s)	25, 161, 555, 2142, 4109
TseFI	GTSAC	3 cut(s)	418, 2621, 4067
TseI	GCWGC	9 cut(s)	320, 874, 1846, 2140, 2365, 2843, 2846, 3168, 3900
Tsp45I	GTSAC	3 cut(s)	418, 2621, 4067
TspGWI	ACGGA	3 cut(s)	1769, 1970, 4043
TspMI	CCCGGG	1 cut(s)	3497
TspRI	CASTG	5 cut(s)	25, 161, 555, 2142, 4109
Tth111I	GACNNNGTC	1 cut(s)	3095
VneI	GTGCAC	1 cut(s)	553
VpaK11BI	GGWCC	4 cut(s)	450, 496, 1400, 3831
XagI	CCTNNNNNAGG	1 cut(s)	3053
XceI	RCATGY	4 cut(s)	94, 1051, 2188, 3951
XhoI	CTCGAG	1 cut(s)	2350
XmaI	CCCGGG	1 cut(s)	3497
XmiI	GTMKAC	2 cut(s)	845, 1427
XmnI	GAANNNNTTC	2 cut(s)	2777, 3557
XspI	CTAG	9 cut(s)	2010, 2174, 2690, 2787, 2967, 3528, 3599, 3626, 3698
ZraI	GACGTC	1 cut(s)	310
ZrmI	AGTACT	5 cut(s)	712, 1565, 1701, 2088, 3785
Zsp2I	ATGCAT	1 cut(s)	2273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.