MD02G1058100.v1.1

RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006890
Physical Location & Seq
Reverse (-)
4680404 .. 4682297
1894 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1058100.v1.1.491

Sequence Viewer

Length: 486 bp
ATGGTCCGACTGGGCCTTTTTTTTAATGGAGACTTGGCAATGAAGAAGATGATTGGTCAAGAAATGGAGGAAGATGCTAGATTGAAGGTTTTCAAGAAGGAATGGTTCGAAGGAAAGGATTGTCTTGACATTGGCTGCAATTCTGGAATCATGACCATTCAAATTGTCATAACTTTGAGCGTGTCGAAATGGATTCATCTGAATTGGGGTGACGATGGATTGATTACATTATTTACAAGGGTTTGGAGTCTACTTCGTCTGGGCGGAATTTTTGTGTTGGAACCTCAACCTTGGACGTCCTACGAAAAGAATTATCGAGTTTCTGAGACAACTAAAGAAAATTATAGAAATCTTATGTTCTATCCGAAAGATTTTCAAGACTTGCTTCTGGATAAAATAGGATTCAGAAAGGTGGAGGACATCGCTTCAAGCGTGTCGGGCAGCAAAACCGGATTCAACAGACCAATTTTAGTGTTCCACAAATGA

Protein Analysis

162

Amino Acids

18.69

Weight (kDa)

8.5

Isoelectric Point (pI)

22.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bin3 PF06859 54 - 161 4.6e-39 Bicoid-interacting protein 3 (Bin3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 250
AciI CCGC 1 cut(s) 264
AcsI RAATTY 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 296
AgsI TTSAA 6 cut(s) 85, 94, 161, 377, 429, 457
Alw26I GTCTC 2 cut(s) 24, 320
AoxI GGCC 1 cut(s) 13
ApeKI GCWGC 2 cut(s) 135, 441
ApoI RAATTY 1 cut(s) 267
Asp700I GAANNNNTTC 1 cut(s) 89
AspS9I GGNCC 2 cut(s) 4, 13
AsuHPI GGTGA 1 cut(s) 221
AsuII TTCGAA 1 cut(s) 108
AvaII GGWCC 1 cut(s) 4
BbvI GCAGC 2 cut(s) 122, 453
BccI CCATC 1 cut(s) 209
BcoDI GTCTC 2 cut(s) 24, 320
BfaI CTAG 1 cut(s) 78
BisI GCNGC 2 cut(s) 136, 442
BlsI GCNGC 2 cut(s) 137, 443
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 2 cut(s) 4, 13
BmiI GGNNCC 1 cut(s) 282
BmrI ACTGGG 1 cut(s) 20
BmsI GCATC 1 cut(s) 64
BmuI ACTGGG 1 cut(s) 20
Bpu14I TTCGAA 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 290
BsaWI WCCGGW 1 cut(s) 449
Bse1I ACTGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 290
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 1 cut(s) 15
BseXI GCAGC 2 cut(s) 122, 453
BshFI GGCC 1 cut(s) 15
BsiSI CCGG 1 cut(s) 450
BsmAI GTCTC 2 cut(s) 24, 320
BsnI GGCC 1 cut(s) 15
Bsp119I TTCGAA 1 cut(s) 108
BspACI CCGC 1 cut(s) 264
BspANI GGCC 1 cut(s) 15
BspCNI CTCAG 1 cut(s) 316
BspHI TCATGA 1 cut(s) 150
BspLI GGNNCC 1 cut(s) 282
BspT104I TTCGAA 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 290
BssNI GRCGYC 1 cut(s) 296
BssT1I CCWWGG 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 108
BstDEI CTNAG 1 cut(s) 324
BstMAI GTCTC 2 cut(s) 24, 320
BstMWI GCNNNNNNNGC 1 cut(s) 438
BstV1I GCAGC 2 cut(s) 122, 453
BsuRI GGCC 1 cut(s) 15
BtgZI GCGATG 1 cut(s) 406
CciI TCATGA 1 cut(s) 150
Cfr13I GGNCC 2 cut(s) 4, 13
CviAII CATG 1 cut(s) 151
CviJI RGCY 2 cut(s) 15, 135
CviKI_1 RGCY 2 cut(s) 15, 135
DdeI CTNAG 1 cut(s) 324
EciI GGCGGA 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 4
EcoT14I CCWWGG 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 1 cut(s) 154
FaiI YATR 4 cut(s) 152, 170, 345, 356
FalI AAGNNNNNCTT 2 cut(s) 369, 401
FatI CATG 1 cut(s) 150
FblI GTMKAC 1 cut(s) 250
Fnu4HI GCNGC 2 cut(s) 136, 442
Fsp4HI GCNGC 2 cut(s) 136, 442
FspBI CTAG 1 cut(s) 78
GluI GCNGC 2 cut(s) 136, 442
HaeIII GGCC 1 cut(s) 15
HapII CCGG 1 cut(s) 450
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 1 cut(s) 154
HinfI GANTC 5 cut(s) 147, 193, 247, 402, 453
HpaII CCGG 1 cut(s) 450
HphI GGTGA 1 cut(s) 221
Hpy166II GTNNAC 1 cut(s) 251
Hpy188I TCNGA 5 cut(s) 8, 201, 325, 366, 407
Hpy188III TCNNGA 7 cut(s) 59, 94, 125, 144, 151, 377, 389
Hpy8I GTNNAC 1 cut(s) 251
HpyAV CCTTC 3 cut(s) 79, 91, 104
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 1 cut(s) 438
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 1 cut(s) 154
LpnPI CCDG 4 cut(s) 129, 245, 374, 463
Lsp1109I GCAGC 2 cut(s) 122, 453
LweI GCATC 1 cut(s) 64
MaeI CTAG 1 cut(s) 78
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 1 cut(s) 209
MboII GAAGA 3 cut(s) 55, 58, 83
MluCI AATT 7 cut(s) 139, 162, 202, 267, 310, 340, 465
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 2 cut(s) 31, 258
MnlI CCTC 3 cut(s) 61, 294, 409
MroXI GAANNNNTTC 1 cut(s) 89
MseI TTAA 1 cut(s) 24
MspI CCGG 1 cut(s) 450
MwoI GCNNNNNNNGC 1 cut(s) 438
NlaIII CATG 1 cut(s) 154
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 209
NspV TTCGAA 1 cut(s) 108
PagI TCATGA 1 cut(s) 150
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PdmI GAANNNNTTC 1 cut(s) 89
PfeI GAWTC 4 cut(s) 147, 193, 402, 453
PkrI GCNGC 2 cut(s) 137, 443
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 282
PspPI GGNCC 2 cut(s) 4, 13
SaqAI TTAA 1 cut(s) 24
SatI GCNGC 2 cut(s) 136, 442
Sau96I GGNCC 2 cut(s) 4, 13
SchI GAGTC 1 cut(s) 256
SetI ASST 5 cut(s) 90, 286, 292, 299, 414
SfaNI GCATC 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 108
SinI GGWCC 1 cut(s) 4
Sse9I AATT 7 cut(s) 139, 162, 202, 267, 310, 340, 465
SsiI CCGC 1 cut(s) 264
SspMI CTAG 1 cut(s) 78
StyI CCWWGG 1 cut(s) 290
TaiI ACGT 1 cut(s) 299
TaqI TCGA 3 cut(s) 108, 185, 316
TasI AATT 7 cut(s) 139, 162, 202, 267, 310, 340, 465
TfiI GAWTC 4 cut(s) 147, 193, 402, 453
Tru1I TTAA 1 cut(s) 24
Tru9I TTAA 1 cut(s) 24
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 2 cut(s) 135, 441
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 2 cut(s) 56, 185
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 89
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.