Rh2BG061000

RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006890
Physical Location & Seq
Reverse (-)
4591585 .. 4605886
14302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG061000.1

Sequence Viewer

Length: 690 bp
ATGGCGCACACTAGTGTATTTGTGGCTCATTTAACGGACAAGTTGTACGTATCTACAGCCAAAAAGTTTTGCTGCCGGAGCATTCTTGGAGTTGACATTGATCCTGATCGAGTTCAGGATGCATACTGGCACCTCCAAAAACTTATGAGAATGAAGAAAGCTAAAATGATACCAGGAAATGCTTCCAAGTTGAAGGTTGGTGAGAGTGCAAATGGGTCAGATTGCAGCATTGAAGGTTCATCAAATGAAGAGACAAAGGATAGCCCAAGGAATTGCTCTTCGGAAGAGACAGATCTATTTGACATAGTCTCGTTTCGGAATGAAGATTTTGTTAATAGTCGAGATCCCCTGAATAAGCATTATGACACCATTCTATGTTTGAGCGTGACAAAATGGATTCATCTGAATTGGGGTGATGATGGCTTGCTTACACTATTTTCAAAGATATGGAGACTACTTCGTCCGGGTGGCATTCTTGTGCTGGAACCTCAACCCTGGAAGTCTTATGATAAGAATCATAAAGTTTCTGAGGCAACAAGAACCAATTATCAAAATATTATATTCCATCCGCAGCTGTTTCAGGATATACTTCTGGATAAGATTGGATTTAGAACAGTGGAAGACATCACTTCCAGCGTGTCGGGCAGCAAATCTGGATTCAACAGACCAATTTTAGTGTTCCAAAAATGA

Protein Analysis

229

Amino Acids

26.08

Weight (kDa)

8.22

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bin3 PF06859 121 - 229 7.3e-44 Bicoid-interacting protein 3 (Bin3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 459
AccB1I GGYRCC 1 cut(s) 129
AciI CCGC 1 cut(s) 569
AclWI GGATC 2 cut(s) 95, 338
AfaI GTAC 1 cut(s) 47
AgsI TTSAA 4 cut(s) 193, 233, 441, 661
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 2 cut(s) 172, 494
AleI CACNNNNGTG 1 cut(s) 12
AluBI AGCT 2 cut(s) 161, 574
AluI AGCT 2 cut(s) 161, 574
Alw26I GTCTC 4 cut(s) 245, 281, 313, 445
AlwI GGATC 2 cut(s) 95, 338
ApeKI GCWGC 4 cut(s) 72, 225, 571, 645
Asp700I GAANNNNTTC 1 cut(s) 181
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 2 cut(s) 212, 425
BanI GGYRCC 1 cut(s) 129
BbsI GAAGAC 1 cut(s) 627
BbvI GCAGC 4 cut(s) 59, 237, 583, 657
BccI CCATC 2 cut(s) 413, 573
BciT130I CCWGG 2 cut(s) 174, 496
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 4 cut(s) 245, 281, 313, 445
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 54
BglII AGATCT 1 cut(s) 292
BisI GCNGC 4 cut(s) 73, 226, 572, 646
BlsI GCNGC 4 cut(s) 74, 227, 573, 647
Bme1390I CCNGG 3 cut(s) 174, 465, 496
BmiI GGNNCC 2 cut(s) 131, 486
BmrFI CCNGG 3 cut(s) 174, 465, 496
BmsI GCATC 1 cut(s) 109
BpiI GAAGAC 1 cut(s) 627
BpuMI CCSGG 1 cut(s) 465
BsaAI YACGTR 1 cut(s) 49
BsaBI GATNNNNATC 2 cut(s) 105, 513
BsaJI CCNNGG 2 cut(s) 266, 494
Bse1I ACTGG 1 cut(s) 131
Bse8I GATNNNNATC 2 cut(s) 105, 513
BseBI CCWGG 2 cut(s) 174, 496
BseDI CCNNGG 2 cut(s) 266, 494
BseGI GGATG 2 cut(s) 124, 565
BseJI GATNNNNATC 2 cut(s) 105, 513
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 1 cut(s) 131
BseXI GCAGC 4 cut(s) 59, 237, 583, 657
BshNI GGYRCC 1 cut(s) 129
BsiSI CCGG 2 cut(s) 76, 464
BsmAI GTCTC 4 cut(s) 245, 281, 313, 445
BsmI GAATGC 2 cut(s) 81, 471
Bsp143I GATC 4 cut(s) 100, 106, 292, 343
BspACI CCGC 1 cut(s) 569
BspCNI CTCAG 1 cut(s) 520
BspLI GGNNCC 2 cut(s) 131, 486
BspPI GGATC 2 cut(s) 95, 338
BspQI GCTCTTC 1 cut(s) 283
BspT107I GGYRCC 1 cut(s) 129
BsrI ACTGG 1 cut(s) 131
BssECI CCNNGG 2 cut(s) 266, 494
BssMI GATC 4 cut(s) 100, 106, 292, 343
BssT1I CCWWGG 1 cut(s) 266
Bst2UI CCWGG 2 cut(s) 174, 496
Bst4CI ACNGT 1 cut(s) 616
Bst6I CTCTTC 3 cut(s) 243, 279, 283
BstBAI YACGTR 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 425
BstDEI CTNAG 1 cut(s) 528
BstF5I GGATG 2 cut(s) 124, 565
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 103, 109, 295, 346
BstMAI GTCTC 4 cut(s) 245, 281, 313, 445
BstMBI GATC 4 cut(s) 100, 106, 292, 343
BstMWI GCNNNNNNNGC 2 cut(s) 78, 642
BstNI CCWGG 2 cut(s) 174, 496
BstSCI CCNGG 3 cut(s) 172, 463, 494
BstSFI CTRYAG 1 cut(s) 54
BstSNI TACGTA 1 cut(s) 49
BstV1I GCAGC 4 cut(s) 59, 237, 583, 657
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 2 cut(s) 292, 343
BstYI RGATCY 2 cut(s) 292, 343
BtsCI GGATG 2 cut(s) 124, 565
BtsIMutI CAGTG 1 cut(s) 621
Cac8I GCNNGC 1 cut(s) 425
CfoI GCGC 1 cut(s) 7
Csp6I GTAC 1 cut(s) 46
CviJI RGCY 6 cut(s) 26, 59, 161, 264, 423, 574
CviKI_1 RGCY 6 cut(s) 26, 59, 161, 264, 423, 574
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 1 cut(s) 528
DpnI GATC 4 cut(s) 102, 108, 294, 345
DpnII GATC 4 cut(s) 100, 106, 292, 343
DrdI GACNNNNNNGTC 1 cut(s) 459
DseDI GACNNNNNNGTC 1 cut(s) 459
Eam1104I CTCTTC 3 cut(s) 243, 279, 283
EarI CTCTTC 3 cut(s) 243, 279, 283
Eco105I TACGTA 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 172, 494
EcoT14I CCWWGG 1 cut(s) 266
EcoT22I ATGCAT 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 266
Fnu4HI GCNGC 4 cut(s) 73, 226, 572, 646
FokI GGATG 2 cut(s) 131, 552
Fsp4HI GCNGC 4 cut(s) 73, 226, 572, 646
FspBI CTAG 1 cut(s) 12
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 73, 226, 572, 646
HapII CCGG 2 cut(s) 76, 464
HhaI GCGC 1 cut(s) 7
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 3 cut(s) 397, 514, 657
HpaII CCGG 2 cut(s) 76, 464
HphI GGTGA 2 cut(s) 212, 425
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 5 cut(s) 220, 283, 318, 405, 529
Hpy188III TCNNGA 6 cut(s) 104, 116, 341, 581, 593, 654
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 2 cut(s) 187, 227
HpyCH4III ACNGT 1 cut(s) 616
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 3 cut(s) 122, 209, 225
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 642
HpyF3I CTNAG 1 cut(s) 528
HpySE526I ACGT 1 cut(s) 48
HspAI GCGC 1 cut(s) 5
Kzo9I GATC 4 cut(s) 100, 106, 292, 343
LguI GCTCTTC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 78
Lsp1109I GCAGC 4 cut(s) 59, 237, 583, 657
LweI GCATC 1 cut(s) 109
MaeI CTAG 1 cut(s) 12
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 385
MalI GATC 4 cut(s) 102, 108, 294, 345
MboI GATC 4 cut(s) 100, 106, 292, 343
MboII GAAGA 6 cut(s) 166, 260, 270, 296, 335, 632
MflI RGATCY 2 cut(s) 292, 343
MluCI AATT 4 cut(s) 271, 406, 544, 669
MnlI CCTC 3 cut(s) 143, 498, 523
Mph1103I ATGCAT 1 cut(s) 124
MroXI GAANNNNTTC 1 cut(s) 181
MseI TTAA 2 cut(s) 32, 333
MslI CAYNNNNRTG 2 cut(s) 12, 476
MspA1I CMGCKG 1 cut(s) 574
MspI CCGG 2 cut(s) 76, 464
MspR9I CCNGG 3 cut(s) 174, 465, 496
Mva1269I GAATGC 2 cut(s) 81, 471
MvaI CCWGG 2 cut(s) 174, 496
MwoI GCNNNNNNNGC 2 cut(s) 78, 642
NciI CCSGG 1 cut(s) 465
NdeII GATC 4 cut(s) 100, 106, 292, 343
NlaIV GGNNCC 2 cut(s) 131, 486
NmuCI GTSAC 1 cut(s) 385
NsiI ATGCAT 1 cut(s) 124
OliI CACNNNNGTG 1 cut(s) 12
PciSI GCTCTTC 1 cut(s) 283
PctI GAATGC 2 cut(s) 81, 471
PdmI GAANNNNTTC 1 cut(s) 181
PfeI GAWTC 3 cut(s) 397, 514, 657
PflFI GACNNNGTC 1 cut(s) 305
PkrI GCNGC 4 cut(s) 74, 227, 573, 647
Ppu21I YACGTR 1 cut(s) 49
Psp6I CCWGG 2 cut(s) 172, 494
PspGI CCWGG 2 cut(s) 172, 494
PspN4I GGNNCC 2 cut(s) 131, 486
PsuI RGATCY 2 cut(s) 292, 343
PsyI GACNNNGTC 1 cut(s) 305
PvuII CAGCTG 1 cut(s) 574
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 2 cut(s) 12, 476
SapI GCTCTTC 1 cut(s) 283
SaqAI TTAA 2 cut(s) 32, 333
SatI GCNGC 4 cut(s) 73, 226, 572, 646
Sau3AI GATC 4 cut(s) 100, 106, 292, 343
ScrFI CCNGG 3 cut(s) 174, 465, 496
SetI ASST 7 cut(s) 51, 135, 163, 198, 238, 490, 576
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 1 cut(s) 54
SmiMI CAYNNNNRTG 2 cut(s) 12, 476
SnaBI TACGTA 1 cut(s) 49
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 4 cut(s) 271, 406, 544, 669
SsiI CCGC 1 cut(s) 569
SspI AATATT 1 cut(s) 556
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 3 cut(s) 172, 463, 494
StyI CCWWGG 1 cut(s) 266
TaaI ACNGT 1 cut(s) 616
TaiI ACGT 1 cut(s) 51
TaqI TCGA 2 cut(s) 109, 340
TasI AATT 4 cut(s) 271, 406, 544, 669
TfiI GAWTC 3 cut(s) 397, 514, 657
Tru1I TTAA 2 cut(s) 32, 333
Tru9I TTAA 2 cut(s) 32, 333
TscAI CASTG 1 cut(s) 621
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 4 cut(s) 72, 225, 571, 645
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 5 cut(s) 167, 228, 261, 336, 389
TspGWI ACGGA 1 cut(s) 50
TspRI CASTG 1 cut(s) 621
Tth111I GACNNNGTC 1 cut(s) 305
XmnI GAANNNNTTC 1 cut(s) 181
XspI CTAG 1 cut(s) 12
Zsp2I ATGCAT 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.