MD02G1060900.v1.1

Protein phosphatase 1 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011350
Physical Location & Seq
Reverse (-)
4879305 .. 4881831
2527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1060900.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCCGACCAGACTGAAGCGCCACCGCCGGGTCAGAATCCCGTAGATCACGATGATCAGACGGCGGACGACGATCCGTCCTCGACGGTCCTCGATCTCACCAGCTTCCAGCTCCACGACCTCGACTCGGTCGAGTTACCGCCGAGTCTAACAGAGTTAGACCTGACGGCGAACCGCCTCACCGGTTTGGACCCTCGGATTGCGACCCTCTCCAACCTCAAAAAGCTCTCTCTCCGTCAGAACCTCATCGAAGACGCCGCCGTCGAACCGATCTCCGGCTGGGACGCCCTCTCCGGTCTCGAGGAGTTGGTGCTGAGGGATAATAAGCTCGGGAAAATACCTGATGTCAGCATATTCACGAAGCTTCTGGTTTTCGACGTTTCGTTCAACGAGATTACATTGTTACATGGATTGGCCAAGGTCACCAGTACCCTCAAGGAGCTGTACGTGTCTAAAAATGAAGTCACCAAGATTGAAGAAATTGAGCACCTCCACGAGCTTCTGATTCTCGAGCTCGGTTCGAATCGATTGCGGGTGATGGAGAATTTGCAGACCTTGACAAAATTGCAGGAGCTGTGGCTGGGGCGGAATCGGATTAAGGTGGTGAATTTGTGTGGGTTGAAATGCATCAAGAAGCTGAGCTTGCAAAGCAATCGGTTAACTTCAATGACGGGATTTGAGGAGTGCGTTGCTCTAGAGGAGTTGTACTTGAGCCATAATGGTATTTCAAAAATGGAAGGCCTCTCAACATTAGTCAACCTACGTGTCCTGGATGTATCGAATAATAAGCTAACATCGGTGGAGGGTATAGAAAGCCTAACAATGTTAGAAGATCTATGGCTTAATGACAACAGCATAGAGTCACTTGAAGGCTTTGCCGAGGTTCTTGCTGGTTCAAGAGAGAAGCTCACTACCATCTATCTCGAAAAAAACCCATGTGCAAAGTCTCCAAACTATGCTACTACCTTGAGACAAATCTTCCCAAATATCGAGCAAATTGATTCTCATATGTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

340

Amino Acids

37.83

Weight (kDa)

4.73

Isoelectric Point (pI)

46.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 48 - 86 2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 48 - 84 4.9e-07 Leucine rich repeat
LRR_4 PF12799 232 - 271 1.6e-09 Leucine Rich repeats (2 copies)
LRR_9 PF14580 236 - 332 3.6e-06 Leucine-rich repeat
LRR_4 PF12799 253 - 293 1.9e-08 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19680
fragaria_vesca FvH4_1g05880 FvH4_1g05880
malus_domestica MD02G1060900.v1.1 MD15G1194400.v1.1
prunus_persica Prupe.7G222100_v2.0.a1 Prupe.7G222100_v2.0.a1
pyrus_communis pycom02g05000 pycom15g17400
rosa_chinensis RchiOBHm_Chr2g0091541 RchiOBHm_Chr2g0091621
rosa_laevigata RLG00000016234
rosa_multiflora Rmu_sc0002407.1_g000003
rosa_roxburghii Rroxscaffold_2G00149950
rosa_rugosa Rorug02G0018600
rosa_samantha Rh2AG063800 Rh2BG063200 Rh2CG064900 Rh2DG063300
rosa_wichuraiana Rw2G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AciI CCGC 7 cut(s) 26, 65, 140, 175, 258, 532, 586
AclWI GGATC 1 cut(s) 68
AcoI YGGCCR 2 cut(s) 3, 414
AcsI RAATTY 2 cut(s) 544, 607
AcuI CTGAAG 1 cut(s) 36
AcyI GRCGYC 2 cut(s) 255, 285
AfaI GTAC 3 cut(s) 430, 446, 707
AfiI CCNNNNNNNGG 3 cut(s) 29, 127, 275
AflIII ACRYGT 2 cut(s) 447, 763
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 7 cut(s) 388, 476, 622, 666, 729, 869, 897
AhdI GACNNNNNGTC 1 cut(s) 76
AjnI CCWGG 1 cut(s) 768
Alw21I GWGCWC 2 cut(s) 489, 516
Alw26I GTCTC 3 cut(s) 302, 951, 964
AlwI GGATC 1 cut(s) 68
AlwNI CAGNNNCTG 1 cut(s) 574
Ama87I CYCGRG 3 cut(s) 299, 329, 509
AoxI GGCC 3 cut(s) 3, 414, 739
ApoI RAATTY 2 cut(s) 544, 607
AsiGI ACCGGT 1 cut(s) 182
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 2 cut(s) 88, 190
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 6 cut(s) 91, 172, 415, 457, 547, 616
AsuII TTCGAA 1 cut(s) 521
AvaI CYCGRG 3 cut(s) 299, 329, 509
AvaII GGWCC 2 cut(s) 88, 190
BalI TGGCCA 1 cut(s) 416
BanII GRGCYC 1 cut(s) 516
BauI CACGAG 1 cut(s) 494
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 2 cut(s) 489, 516
BbvCI CCTCAGC 1 cut(s) 314
BccI CCATC 2 cut(s) 532, 923
BceAI ACGGC 3 cut(s) 78, 183, 245
BcgI CGANNNNNNTGC 6 cut(s) 511, 545, 635, 669, 869, 903
BciT130I CCWGG 1 cut(s) 770
BclI TGATCA 1 cut(s) 55
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 3 cut(s) 302, 951, 964
BfaI CTAG 1 cut(s) 695
BfoI RGCGCY 1 cut(s) 23
BglII AGATCT 1 cut(s) 832
BisI GCNGC 1 cut(s) 258
BlpI GCTNAGC 1 cut(s) 638
BlsI GCNGC 1 cut(s) 259
Bme1390I CCNGG 2 cut(s) 30, 770
Bme18I GGWCC 2 cut(s) 88, 190
BmeRI GACNNNNNGTC 1 cut(s) 76
BmeT110I CYCGRG 3 cut(s) 299, 329, 509
BmgT120I GGNCC 2 cut(s) 88, 190
BmiI GGNNCC 1 cut(s) 192
BmrFI CCNGG 2 cut(s) 30, 770
BmsI GCATC 1 cut(s) 636
BpiI GAAGAC 1 cut(s) 258
BplI GAGNNNNNCTC 2 cut(s) 891, 923
Bpu10I CCTNAGC 1 cut(s) 314
Bpu1102I GCTNAGC 1 cut(s) 638
Bpu14I TTCGAA 1 cut(s) 521
BpuEI CTTGAG 3 cut(s) 419, 730, 988
BpuMI CCSGG 1 cut(s) 30
Bsa29I ATCGAT 1 cut(s) 526
BsaAI YACGTR 2 cut(s) 448, 764
BsaHI GRCGYC 2 cut(s) 255, 285
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 3 cut(s) 194, 417, 879
BsaWI WCCGGW 2 cut(s) 182, 293
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 127, 275
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 426
BseBI CCWGG 1 cut(s) 770
BseCI ATCGAT 1 cut(s) 526
BseDI CCNNGG 3 cut(s) 194, 417, 879
BseGI GGATG 1 cut(s) 778
BseLI CCNNNNNNNGG 3 cut(s) 29, 127, 275
BseMII CTCAG 2 cut(s) 305, 629
BseNI ACTGG 1 cut(s) 426
BseRI GAGGAG 3 cut(s) 317, 695, 713
BseYI CCCAGC 2 cut(s) 279, 580
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 3 cut(s) 5, 416, 741
BshTI ACCGGT 1 cut(s) 182
BshVI ATCGAT 1 cut(s) 526
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 489, 516
BsiHKCI CYCGRG 3 cut(s) 299, 329, 509
BsiSI CCGG 4 cut(s) 29, 183, 276, 294
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 3 cut(s) 29, 127, 275
BsmAI GTCTC 3 cut(s) 302, 951, 964
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 3 cut(s) 5, 416, 741
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 3 cut(s) 299, 329, 509
Bsp119I TTCGAA 1 cut(s) 521
Bsp1286I GDGCHC 2 cut(s) 489, 516
Bsp143I GATC 6 cut(s) 46, 55, 73, 94, 270, 832
Bsp1720I GCTNAGC 1 cut(s) 638
BspACI CCGC 7 cut(s) 26, 65, 140, 175, 258, 532, 586
BspANI GGCC 3 cut(s) 5, 416, 741
BspCNI CTCAG 2 cut(s) 306, 630
BspDI ATCGAT 1 cut(s) 526
BspLI GGNNCC 1 cut(s) 192
BspPI GGATC 1 cut(s) 68
BspT104I TTCGAA 1 cut(s) 521
BspTNI GGTCTC 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 1 cut(s) 426
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 3 cut(s) 194, 417, 879
BssMI GATC 6 cut(s) 46, 55, 73, 94, 270, 832
BssNI GRCGYC 2 cut(s) 255, 285
BssSI CACGAG 1 cut(s) 494
BssT1I CCWWGG 1 cut(s) 417
Bst2BI CACGAG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 770
Bst4CI ACNGT 1 cut(s) 88
BstACI GRCGYC 2 cut(s) 255, 285
BstBAI YACGTR 2 cut(s) 448, 764
BstBI TTCGAA 1 cut(s) 521
BstC8I GCNNGC 1 cut(s) 644
BstDEI CTNAG 2 cut(s) 314, 638
BstEII GGTNACC 1 cut(s) 421
BstF5I GGATG 1 cut(s) 778
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 6 cut(s) 49, 58, 76, 97, 273, 835
BstMAI GTCTC 3 cut(s) 302, 951, 964
BstMBI GATC 6 cut(s) 46, 55, 73, 94, 270, 832
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 643, 648
BstNI CCWGG 1 cut(s) 770
BstPI GGTNACC 1 cut(s) 421
BstSCI CCNGG 2 cut(s) 28, 768
BstV2I GAAGAC 1 cut(s) 258
BstX2I RGATCY 1 cut(s) 832
BstYI RGATCY 1 cut(s) 832
Bsu15I ATCGAT 1 cut(s) 526
BsuRI GGCC 3 cut(s) 5, 416, 741
BsuTUI ATCGAT 1 cut(s) 526
BtsCI GGATG 1 cut(s) 778
Cac8I GCNNGC 1 cut(s) 644
CaiI CAGNNNCTG 1 cut(s) 574
CfoI GCGC 1 cut(s) 22
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 88, 190
ClaI ATCGAT 1 cut(s) 526
CseI GACGC 2 cut(s) 263, 293
Csp6I GTAC 3 cut(s) 429, 445, 706
CspAI ACCGGT 1 cut(s) 182
CviAII CATG 2 cut(s) 407, 936
CviQI GTAC 3 cut(s) 429, 445, 706
DdeI CTNAG 2 cut(s) 314, 638
DpnI GATC 6 cut(s) 48, 57, 75, 96, 272, 834
DpnII GATC 6 cut(s) 46, 55, 73, 94, 270, 832
DrdI GACNNNNNNGTC 1 cut(s) 260
DriI GACNNNNNGTC 1 cut(s) 76
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 2 cut(s) 3, 414
Eam1105I GACNNNNNGTC 1 cut(s) 76
EciI GGCGGA 2 cut(s) 80, 601
Ecl136II GAGCTC 1 cut(s) 514
Eco130I CCWWGG 1 cut(s) 417
Eco147I AGGCCT 1 cut(s) 741
Eco24I GRGCYC 1 cut(s) 516
Eco31I GGTCTC 1 cut(s) 302
Eco47I GGWCC 2 cut(s) 88, 190
Eco53kI GAGCTC 1 cut(s) 514
Eco57I CTGAAG 1 cut(s) 36
Eco88I CYCGRG 3 cut(s) 299, 329, 509
Eco91I GGTNACC 1 cut(s) 421
EcoICRI GAGCTC 1 cut(s) 514
EcoO65I GGTNACC 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 768
EcoT14I CCWWGG 1 cut(s) 417
EcoT22I ATGCAT 1 cut(s) 629
EcoT38I GRGCYC 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 2 cut(s) 410, 939
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 1 cut(s) 296
FatI CATG 2 cut(s) 406, 935
FauI CCCGC 1 cut(s) 525
FauNDI CATATG 1 cut(s) 1008
FbaI TGATCA 1 cut(s) 55
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 1 cut(s) 785
FriOI GRGCYC 1 cut(s) 516
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 1 cut(s) 695
GlaI GCGC 1 cut(s) 21
GluI GCNGC 1 cut(s) 258
GsaI CCCAGC 2 cut(s) 283, 584
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 3 cut(s) 5, 416, 741
HapII CCGG 4 cut(s) 29, 183, 276, 294
HgaI GACGC 2 cut(s) 263, 293
HhaI GCGC 1 cut(s) 22
Hin1I GRCGYC 2 cut(s) 255, 285
Hin1II CATG 2 cut(s) 410, 939
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 2 cut(s) 660, 757
HindII GTYRAC 2 cut(s) 660, 757
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 8 cut(s) 37, 125, 145, 505, 523, 589, 860, 1001
HpaI GTTAAC 1 cut(s) 660
HpaII CCGG 4 cut(s) 29, 183, 276, 294
HphI GGTGA 6 cut(s) 91, 172, 415, 457, 547, 616
Hpy166II GTNNAC 2 cut(s) 660, 757
Hpy188I TCNGA 6 cut(s) 36, 60, 198, 240, 504, 594
Hpy188III TCNNGA 9 cut(s) 50, 299, 331, 358, 509, 631, 695, 897, 923
Hpy8I GTNNAC 2 cut(s) 660, 757
Hpy99I CGWCG 4 cut(s) 74, 88, 266, 380
HpyAV CCTTC 2 cut(s) 731, 863
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 3 cut(s) 378, 447, 763
HpyCH4V TGCA 6 cut(s) 550, 568, 627, 646, 941, 1016
HpyF10VI GCNNNNNNNGC 2 cut(s) 643, 648
HpyF3I CTNAG 2 cut(s) 314, 638
HpySE526I ACGT 3 cut(s) 378, 447, 763
Hsp92I GRCGYC 2 cut(s) 255, 285
Hsp92II CATG 2 cut(s) 410, 939
HspAI GCGC 1 cut(s) 20
Ksp22I TGATCA 1 cut(s) 55
KspAI GTTAAC 1 cut(s) 660
Kzo9I GATC 6 cut(s) 46, 55, 73, 94, 270, 832
LmnI GCTCC 3 cut(s) 117, 439, 571
LweI GCATC 1 cut(s) 636
MaeI CTAG 1 cut(s) 695
MaeII ACGT 3 cut(s) 378, 447, 763
MaeIII GTNAC 5 cut(s) 135, 402, 421, 463, 861
MalI GATC 6 cut(s) 48, 57, 75, 96, 272, 834
MboI GATC 6 cut(s) 46, 55, 73, 94, 270, 832
MboII GAAGA 4 cut(s) 263, 488, 842, 970
MflI RGATCY 1 cut(s) 832
MhlI GDGCHC 2 cut(s) 489, 516
MlsI TGGCCA 1 cut(s) 416
MluCI AATT 5 cut(s) 480, 544, 563, 607, 996
MluNI TGGCCA 1 cut(s) 416
MlyI GAGTC 3 cut(s) 119, 154, 869
MmeI TCCRAC 1 cut(s) 237
Mox20I TGGCCA 1 cut(s) 416
Mph1103I ATGCAT 1 cut(s) 629
MscI TGGCCA 1 cut(s) 416
MseI TTAA 3 cut(s) 597, 659, 843
Msp20I TGGCCA 1 cut(s) 416
MspI CCGG 4 cut(s) 29, 183, 276, 294
MspR9I CCNGG 2 cut(s) 30, 770
MvaI CCWGG 1 cut(s) 770
MwoI GCNNNNNNNGC 2 cut(s) 643, 648
NciI CCSGG 1 cut(s) 30
NdeI CATATG 1 cut(s) 1008
NdeII GATC 6 cut(s) 46, 55, 73, 94, 270, 832
NlaIII CATG 2 cut(s) 410, 939
NlaIV GGNNCC 1 cut(s) 192
NmeAIII GCCGAG 2 cut(s) 168, 904
NmuCI GTSAC 3 cut(s) 421, 463, 861
NsiI ATGCAT 1 cut(s) 629
NspV TTCGAA 1 cut(s) 521
PaeR7I CTCGAG 2 cut(s) 299, 509
PceI AGGCCT 1 cut(s) 741
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 261
PfeI GAWTC 5 cut(s) 37, 505, 523, 589, 1001
PflFI GACNNNGTC 1 cut(s) 128
PfoI TCCNGGA 1 cut(s) 768
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 3 cut(s) 119, 153, 868
PpsI GAGTC 3 cut(s) 119, 153, 868
Ppu21I YACGTR 2 cut(s) 448, 764
Psp124BI GAGCTC 1 cut(s) 516
Psp6I CCWGG 1 cut(s) 768
PspEI GGTNACC 1 cut(s) 421
PspFI CCCAGC 2 cut(s) 279, 580
PspGI CCWGG 1 cut(s) 768
PspN4I GGNNCC 1 cut(s) 192
PspPI GGNCC 2 cut(s) 88, 190
PstNI CAGNNNCTG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 832
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 3 cut(s) 430, 446, 707
RsaNI GTAC 3 cut(s) 429, 445, 706
SacI GAGCTC 1 cut(s) 516
SaqAI TTAA 3 cut(s) 597, 659, 843
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 6 cut(s) 46, 55, 73, 94, 270, 832
Sau96I GGNCC 2 cut(s) 88, 190
SchI GAGTC 3 cut(s) 119, 154, 869
ScrFI CCNGG 2 cut(s) 30, 770
SduI GDGCHC 2 cut(s) 489, 516
SfaNI GCATC 1 cut(s) 636
Sfr274I CTCGAG 2 cut(s) 299, 509
SfuI TTCGAA 1 cut(s) 521
SinI GGWCC 2 cut(s) 88, 190
SlaI CTCGAG 2 cut(s) 299, 509
SmlI CTYRAG 5 cut(s) 299, 434, 509, 709, 967
SmoI CTYRAG 5 cut(s) 299, 434, 509, 709, 967
Sse9I AATT 5 cut(s) 480, 544, 563, 607, 996
SseBI AGGCCT 1 cut(s) 741
SsiI CCGC 7 cut(s) 26, 65, 140, 175, 258, 532, 586
SspMI CTAG 1 cut(s) 695
SstI GAGCTC 1 cut(s) 516
StuI AGGCCT 1 cut(s) 741
StyD4I CCNGG 2 cut(s) 28, 768
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 3 cut(s) 381, 450, 766
TaqII GACCGA 1 cut(s) 118
TasI AATT 5 cut(s) 480, 544, 563, 607, 996
TatI WGTACW 1 cut(s) 705
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 5 cut(s) 37, 505, 523, 589, 1001
Tru1I TTAA 3 cut(s) 597, 659, 843
Tru9I TTAA 3 cut(s) 597, 659, 843
TseFI GTSAC 3 cut(s) 421, 463, 861
Tsp45I GTSAC 3 cut(s) 421, 463, 861
TspDTI ATGAA 1 cut(s) 474
TspGWI ACGGA 2 cut(s) 66, 224
Tth111I GACNNNGTC 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 88, 190
XapI RAATTY 2 cut(s) 544, 607
XbaI TCTAGA 1 cut(s) 694
XhoI CTCGAG 2 cut(s) 299, 509
XspI CTAG 1 cut(s) 695
Zsp2I ATGCAT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.