pycom15g17400

Protein phosphatase 1 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011350
Physical Location & Seq
Reverse (-)
12274338 .. 12276383
2046 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g17400.1

Sequence Viewer

Length: 1020 bp
ATGGCCGACCAGACTTTAGCGCCACCGCCGGATCCGAATCCCGAAGAGCACGACGATCAGACGGCGGAGGATGATTCTTCCTCGACGGTCCTCGACCTCACCAGCTTCCAGCTCCATGACCTCGACTCGGTCGAGTTACCGCCGAGTCTAACTGAGTTAGACCTAACGGCGAACCGCCTCACCAGCTTGGACACTCGGATTGCGAACCTCTCCAACCTCAAAAAGCTCTCACTCCGTCAGAACCTCATCGATGACGCCGCCGTCGAACCGATCTCCGGCTGGGACGCCCTCTCCGGTATAGAGGAGTTGGTGCTGAGGGATAATAAGTTGGGGAAAATACCAGATGTTAGCATATTCACGAAGCTTCTGGTTTTCGATTTTTCTTTCAACGAGATTACGTCGTTACATGGATTGTCCAAGGTCACCAGTACCCTCAAGGAGCTCTACGTGTCGAAAAATGAAGTTACCAAGATTGAAGAAATTGAGCACCTCCATGAGCTTCTGATTCTCGAGCTCGGTTCGAATCGATTGCGGGTGATGGAGAGTTTGCAGAACATGACAAAGTTGCAGGAGCTGTGGCTGGGGCGGAATCGGATTAAGGTGGTGAATTTGTGTGGGTTGAAATGCATCAAGAAGCTGAGCTTGCAGAGCAATCGGTTAACTTCAATGACAGGATTTGAGGAGTGTGTTGCTCTAGAGGAGTTGTACTTAAGCCATAATGGTATTTCGAAAATGGAAGGCCTCTCAACGTCAGTCAACCTACGTGTCTTGGATGTATCGAATAATAAGCTAACATCGGTGGAGAGTATTGAAAACCTTACAGTGTTAGAAGATCTATGGCTTAATGACAACAGCATAGAGACAGTTGAAGGCTTTGCCGAGGTTGTTGCTGGTTCTAGAGAGAAGCTCACTACAATCTATCTCGAAAATAACCCATGTGCAAAGTCTCCAAACTATGCTACTGCCTTGAGACAAATCTTCCCAAACATAGAGCAAATTGATTCTCATGTATTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

340

Amino Acids

37.89

Weight (kDa)

4.66

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 48 - 86 2.2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 48 - 84 8.3e-07 Leucine rich repeat
LRR_4 PF12799 253 - 293 1.2e-08 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19680
fragaria_vesca FvH4_1g05880 FvH4_1g05880
malus_domestica MD02G1060900.v1.1 MD15G1194400.v1.1
prunus_persica Prupe.7G222100_v2.0.a1 Prupe.7G222100_v2.0.a1
pyrus_communis pycom02g05000 pycom15g17400
rosa_chinensis RchiOBHm_Chr2g0091541 RchiOBHm_Chr2g0091621
rosa_laevigata RLG00000016234
rosa_multiflora Rmu_sc0002407.1_g000003
rosa_roxburghii Rroxscaffold_2G00149950
rosa_rugosa Rorug02G0018600
rosa_samantha Rh2AG063800 Rh2BG063200 Rh2CG064900 Rh2DG063300
rosa_wichuraiana Rw2G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AciI CCGC 7 cut(s) 26, 65, 140, 175, 258, 532, 586
AclWI GGATC 2 cut(s) 26, 39
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 607
AcyI GRCGYC 2 cut(s) 255, 285
AfaI GTAC 2 cut(s) 430, 707
AfiI CCNNNNNNNGG 2 cut(s) 127, 275
AflII CTTAAG 1 cut(s) 709
AflIII ACRYGT 2 cut(s) 447, 763
AgsI TTSAA 6 cut(s) 388, 476, 622, 666, 812, 869
Alw21I GWGCWC 4 cut(s) 51, 444, 489, 516
Alw26I GTCTC 3 cut(s) 854, 951, 964
AlwI GGATC 2 cut(s) 26, 39
AlwNI CAGNNNCTG 1 cut(s) 574
Ama87I CYCGRG 1 cut(s) 509
AoxI GGCC 2 cut(s) 3, 739
ApoI RAATTY 1 cut(s) 607
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 88
AsuHPI GGTGA 5 cut(s) 91, 172, 415, 547, 616
AsuII TTCGAA 2 cut(s) 521, 728
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 1 cut(s) 88
BamHI GGATCC 1 cut(s) 31
BanII GRGCYC 2 cut(s) 444, 516
Bbv12I GWGCWC 4 cut(s) 51, 444, 489, 516
BbvCI CCTCAGC 1 cut(s) 314
BccI CCATC 1 cut(s) 532
BceAI ACGGC 3 cut(s) 78, 183, 245
BcgI CGANNNNNNTGC 6 cut(s) 511, 545, 635, 669, 869, 903
BcoDI GTCTC 3 cut(s) 854, 951, 964
BfaI CTAG 2 cut(s) 695, 897
BfoI RGCGCY 1 cut(s) 23
BfrI CTTAAG 1 cut(s) 709
BglII AGATCT 1 cut(s) 832
BisI GCNGC 1 cut(s) 258
BlpI GCTNAGC 1 cut(s) 638
BlsI GCNGC 1 cut(s) 259
Bme18I GGWCC 1 cut(s) 88
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 1 cut(s) 33
BmsI GCATC 1 cut(s) 636
BplI GAGNNNNNCTC 2 cut(s) 891, 923
Bpu10I CCTNAGC 1 cut(s) 314
Bpu1102I GCTNAGC 1 cut(s) 638
Bpu14I TTCGAA 2 cut(s) 521, 728
BpuEI CTTGAG 2 cut(s) 419, 988
Bsa29I ATCGAT 2 cut(s) 249, 526
BsaAI YACGTR 2 cut(s) 448, 764
BsaBI GATNNNNATC 1 cut(s) 36
BsaHI GRCGYC 2 cut(s) 255, 285
BsaJI CCNNGG 2 cut(s) 417, 879
BsaWI WCCGGW 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 275
Bse1I ACTGG 1 cut(s) 426
Bse8I GATNNNNATC 1 cut(s) 36
BseCI ATCGAT 2 cut(s) 249, 526
BseDI CCNNGG 2 cut(s) 417, 879
BseGI GGATG 2 cut(s) 76, 778
BseJI GATNNNNATC 1 cut(s) 36
BseLI CCNNNNNNNGG 2 cut(s) 127, 275
BseMII CTCAG 3 cut(s) 144, 305, 629
BseNI ACTGG 1 cut(s) 426
BseRI GAGGAG 3 cut(s) 317, 695, 713
BseYI CCCAGC 2 cut(s) 279, 580
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 2 cut(s) 5, 741
BshVI ATCGAT 2 cut(s) 249, 526
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 4 cut(s) 51, 444, 489, 516
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 3 cut(s) 29, 276, 294
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 127, 275
BsmAI GTCTC 3 cut(s) 854, 951, 964
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 2 cut(s) 5, 741
BsoBI CYCGRG 1 cut(s) 509
Bsp119I TTCGAA 2 cut(s) 521, 728
Bsp1286I GDGCHC 4 cut(s) 51, 444, 489, 516
Bsp143I GATC 4 cut(s) 31, 55, 270, 832
Bsp1720I GCTNAGC 1 cut(s) 638
BspACI CCGC 7 cut(s) 26, 65, 140, 175, 258, 532, 586
BspANI GGCC 2 cut(s) 5, 741
BspCNI CTCAG 3 cut(s) 145, 306, 630
BspDI ATCGAT 2 cut(s) 249, 526
BspLI GGNNCC 1 cut(s) 33
BspPI GGATC 2 cut(s) 26, 39
BspQI GCTCTTC 1 cut(s) 39
BspT104I TTCGAA 2 cut(s) 521, 728
BspTI CTTAAG 1 cut(s) 709
BsrI ACTGG 1 cut(s) 426
BssECI CCNNGG 2 cut(s) 417, 879
BssMI GATC 4 cut(s) 31, 55, 270, 832
BssNI GRCGYC 2 cut(s) 255, 285
BssT1I CCWWGG 1 cut(s) 417
Bst4CI ACNGT 3 cut(s) 88, 823, 865
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 2 cut(s) 255, 285
BstAFI CTTAAG 1 cut(s) 709
BstBAI YACGTR 2 cut(s) 448, 764
BstBI TTCGAA 2 cut(s) 521, 728
BstC8I GCNNGC 1 cut(s) 644
BstDEI CTNAG 3 cut(s) 153, 314, 638
BstEII GGTNACC 1 cut(s) 421
BstF5I GGATG 2 cut(s) 76, 778
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 4 cut(s) 34, 58, 273, 835
BstMAI GTCTC 3 cut(s) 854, 951, 964
BstMBI GATC 4 cut(s) 31, 55, 270, 832
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 3 cut(s) 183, 643, 648
BstPI GGTNACC 1 cut(s) 421
BstX2I RGATCY 2 cut(s) 31, 832
BstYI RGATCY 2 cut(s) 31, 832
Bsu15I ATCGAT 2 cut(s) 249, 526
BsuRI GGCC 2 cut(s) 5, 741
BsuTUI ATCGAT 2 cut(s) 249, 526
BtsCI GGATG 2 cut(s) 76, 778
BtsIMutI CAGTG 1 cut(s) 828
Cac8I GCNNGC 1 cut(s) 644
CaiI CAGNNNCTG 1 cut(s) 574
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 88
ClaI ATCGAT 2 cut(s) 249, 526
CseI GACGC 2 cut(s) 263, 293
Csp6I GTAC 2 cut(s) 429, 706
CviAII CATG 6 cut(s) 116, 407, 494, 556, 936, 1007
CviQI GTAC 2 cut(s) 429, 706
DdeI CTNAG 3 cut(s) 153, 314, 638
DpnI GATC 4 cut(s) 33, 57, 272, 834
DpnII GATC 4 cut(s) 31, 55, 270, 832
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EciI GGCGGA 2 cut(s) 80, 601
Ecl136II GAGCTC 2 cut(s) 442, 514
Eco130I CCWWGG 1 cut(s) 417
Eco147I AGGCCT 1 cut(s) 741
Eco24I GRGCYC 2 cut(s) 444, 516
Eco47I GGWCC 1 cut(s) 88
Eco53kI GAGCTC 2 cut(s) 442, 514
Eco88I CYCGRG 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 421
EcoICRI GAGCTC 2 cut(s) 442, 514
EcoO65I GGTNACC 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 417
EcoT22I ATGCAT 1 cut(s) 629
EcoT38I GRGCYC 2 cut(s) 444, 516
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 6 cut(s) 119, 410, 497, 559, 939, 1010
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 1 cut(s) 296
FatI CATG 6 cut(s) 115, 406, 493, 555, 935, 1006
FauI CCCGC 1 cut(s) 525
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 2 cut(s) 83, 785
FriOI GRGCYC 2 cut(s) 444, 516
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 2 cut(s) 695, 897
GlaI GCGC 1 cut(s) 21
GluI GCNGC 1 cut(s) 258
GsaI CCCAGC 2 cut(s) 283, 584
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 2 cut(s) 5, 741
HapII CCGG 3 cut(s) 29, 276, 294
HgaI GACGC 2 cut(s) 263, 293
HhaI GCGC 1 cut(s) 22
Hin1I GRCGYC 2 cut(s) 255, 285
Hin1II CATG 6 cut(s) 119, 410, 497, 559, 939, 1010
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 2 cut(s) 660, 757
HindII GTYRAC 2 cut(s) 660, 757
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 8 cut(s) 37, 74, 125, 145, 505, 523, 589, 1001
HpaI GTTAAC 1 cut(s) 660
HpaII CCGG 3 cut(s) 29, 276, 294
HphI GGTGA 5 cut(s) 91, 172, 415, 547, 616
Hpy166II GTNNAC 2 cut(s) 660, 757
Hpy188I TCNGA 6 cut(s) 36, 60, 198, 240, 504, 594
Hpy188III TCNNGA 7 cut(s) 41, 358, 509, 631, 695, 897, 923
Hpy8I GTNNAC 2 cut(s) 660, 757
Hpy99I CGWCG 4 cut(s) 56, 88, 266, 403
HpyAV CCTTC 2 cut(s) 731, 863
HpyCH4III ACNGT 3 cut(s) 88, 823, 865
HpyCH4IV ACGT 4 cut(s) 398, 447, 749, 763
HpyCH4V TGCA 5 cut(s) 550, 568, 627, 646, 941
HpyF10VI GCNNNNNNNGC 3 cut(s) 183, 643, 648
HpyF3I CTNAG 3 cut(s) 153, 314, 638
HpySE526I ACGT 4 cut(s) 398, 447, 749, 763
Hsp92I GRCGYC 2 cut(s) 255, 285
Hsp92II CATG 6 cut(s) 119, 410, 497, 559, 939, 1010
HspAI GCGC 1 cut(s) 20
KspAI GTTAAC 1 cut(s) 660
Kzo9I GATC 4 cut(s) 31, 55, 270, 832
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 117, 439, 571
LweI GCATC 1 cut(s) 636
MaeI CTAG 2 cut(s) 695, 897
MaeII ACGT 4 cut(s) 398, 447, 749, 763
MaeIII GTNAC 4 cut(s) 135, 402, 421, 463
MalI GATC 4 cut(s) 33, 57, 272, 834
MboI GATC 4 cut(s) 31, 55, 270, 832
MboII GAAGA 5 cut(s) 56, 69, 488, 842, 970
MflI RGATCY 2 cut(s) 31, 832
MhlI GDGCHC 4 cut(s) 51, 444, 489, 516
MluCI AATT 3 cut(s) 480, 607, 996
MlyI GAGTC 2 cut(s) 119, 154
MmeI TCCRAC 1 cut(s) 237
Mph1103I ATGCAT 1 cut(s) 629
MseI TTAA 5 cut(s) 597, 659, 710, 843, 1018
MslI CAYNNNNRTG 1 cut(s) 492
MspCI CTTAAG 1 cut(s) 709
MspI CCGG 3 cut(s) 29, 276, 294
MwoI GCNNNNNNNGC 3 cut(s) 183, 643, 648
NdeII GATC 4 cut(s) 31, 55, 270, 832
NlaIII CATG 6 cut(s) 119, 410, 497, 559, 939, 1010
NlaIV GGNNCC 1 cut(s) 33
NmeAIII GCCGAG 2 cut(s) 168, 904
NmuCI GTSAC 1 cut(s) 421
NsiI ATGCAT 1 cut(s) 629
NspV TTCGAA 2 cut(s) 521, 728
PaeR7I CTCGAG 1 cut(s) 509
PceI AGGCCT 1 cut(s) 741
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 261
PfeI GAWTC 6 cut(s) 37, 74, 505, 523, 589, 1001
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 2 cut(s) 119, 153
PpsI GAGTC 2 cut(s) 119, 153
Ppu21I YACGTR 2 cut(s) 448, 764
Psp124BI GAGCTC 2 cut(s) 444, 516
PspEI GGTNACC 1 cut(s) 421
PspFI CCCAGC 2 cut(s) 279, 580
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 574
PsuI RGATCY 2 cut(s) 31, 832
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 2 cut(s) 430, 707
RsaNI GTAC 2 cut(s) 429, 706
RseI CAYNNNNRTG 1 cut(s) 492
SacI GAGCTC 2 cut(s) 444, 516
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 5 cut(s) 597, 659, 710, 843, 1018
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 4 cut(s) 31, 55, 270, 832
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 2 cut(s) 119, 154
SduI GDGCHC 4 cut(s) 51, 444, 489, 516
SfaNI GCATC 1 cut(s) 636
Sfr274I CTCGAG 1 cut(s) 509
SfuI TTCGAA 2 cut(s) 521, 728
SinI GGWCC 1 cut(s) 88
SlaI CTCGAG 1 cut(s) 509
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 4 cut(s) 434, 509, 709, 967
SmoI CTYRAG 4 cut(s) 434, 509, 709, 967
Sse9I AATT 3 cut(s) 480, 607, 996
SseBI AGGCCT 1 cut(s) 741
SsiI CCGC 7 cut(s) 26, 65, 140, 175, 258, 532, 586
SspMI CTAG 2 cut(s) 695, 897
SstI GAGCTC 2 cut(s) 444, 516
StuI AGGCCT 1 cut(s) 741
StyI CCWWGG 1 cut(s) 417
TaaI ACNGT 3 cut(s) 88, 823, 865
TaiI ACGT 4 cut(s) 401, 450, 752, 766
TaqII GACCGA 1 cut(s) 118
TasI AATT 3 cut(s) 480, 607, 996
TatI WGTACW 1 cut(s) 705
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 6 cut(s) 37, 74, 505, 523, 589, 1001
Tru1I TTAA 5 cut(s) 597, 659, 710, 843, 1018
Tru9I TTAA 5 cut(s) 597, 659, 710, 843, 1018
TscAI CASTG 1 cut(s) 828
TseFI GTSAC 1 cut(s) 421
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 1 cut(s) 474
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 1 cut(s) 828
Tth111I GACNNNGTC 1 cut(s) 128
Vha464I CTTAAG 1 cut(s) 709
VpaK11BI GGWCC 1 cut(s) 88
XapI RAATTY 1 cut(s) 607
XbaI TCTAGA 2 cut(s) 694, 896
XhoI CTCGAG 1 cut(s) 509
XspI CTAG 2 cut(s) 695, 897
Zsp2I ATGCAT 1 cut(s) 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.