MD15G1194400.v1.1

Protein phosphatase 1 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011350
Physical Location & Seq
Reverse (-)
15361574 .. 15364057
2484 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1194400.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGGCCGACCAGACTTTAGCACCACTGCCGGATCCGAATCCCGAAGAGCACGACGATCAGACGGCGGAGGATGATTCTTCCTCGACGGTCCTCGACCTCACCAGTTTCCAGCTCCATGACCTCGACTCGGTCGAGTTACCGCCGAGTCTAACTGAGTTAGACATAACTGCAAACCGCCTCACCAGCTTAGACACTCGGATTGCGAACCTCTCCAACCTCAAAAAGCTGTCACTCCGTCAGAACCTCATCGATGACGCCGCCGTCGAACCGATATCCGGCTGGGACGCCCTCTCCGGTCTCGAGTTGGTGCTGAGGGATAATAAGTTGGGGAAAATACCAGATGTTAGCATATTCACGAAGCTTCTGGTTTTCGACTTTTCTTTCAACGAGATTACGTCGTTACATGGATTGTCCAAGGTCACCGGTACCCTCAAGGAGCTCTACGTATCGAAAAATGAAGTTACCAAGATTGAAGAAATTGAGCACCTCCATGAACTTCTGATTCTCGAGCTCGGTTCGAATCGATTGCGGGTGATGGAGAGTTTGCAGAACATGACAAAGTTGCAGGAGCTGTGGCTGGGGCGGAATCGGATTAAGGTGGTGAATTTGTGTGGGTTGAAATGCATCAAGAAGCTGAGCTTGCAGAGCAATCGGTTGACTTCAATGACGGGATTTGAGGAGTGTGTTGCTCTAGAGGAGTTGTACTTAAGCCATAATGGTATTTCGAAAATGGAAGGCCTCTCAACGTTAGTCAACCTACGTGTATTGGATGTATCAAATAATAAGCTAGCATCGGTGGAGGGTATTGAAAACCTTACAGTGTTAGAAGATCTATGGCTTAATGACAACAGCGTAGAGTCACTTGAAGGCTTTGCCAAGGTTGTTGCTGGTTCAAGAGAGAAGCTCACTACCATCTATCTCGAAAATAACCCATGTGCAAAATCTCCAAACTATGCTACTGCCTTGAGGCAAATCTTCTCAAACATAGAGCAAATTGATTCTCATGTATTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

339

Amino Acids

37.69

Weight (kDa)

4.76

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 48 - 85 8e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 48 - 85 8.7e-06 Leucine rich repeat
LRR_8 PF13855 210 - 263 8.2e-07 Leucine rich repeat
LRR_4 PF12799 231 - 271 3.6e-09 Leucine Rich repeats (2 copies)
LRR_4 PF12799 252 - 292 7.1e-08 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19680
fragaria_vesca FvH4_1g05880 FvH4_1g05880
malus_domestica MD02G1060900.v1.1 MD15G1194400.v1.1
prunus_persica Prupe.7G222100_v2.0.a1 Prupe.7G222100_v2.0.a1
pyrus_communis pycom02g05000 pycom15g17400
rosa_chinensis RchiOBHm_Chr2g0091541 RchiOBHm_Chr2g0091621
rosa_laevigata RLG00000016234
rosa_multiflora Rmu_sc0002407.1_g000003
rosa_roxburghii Rroxscaffold_2G00149950
rosa_rugosa Rorug02G0018600
rosa_samantha Rh2AG063800 Rh2BG063200 Rh2CG064900 Rh2DG063300
rosa_wichuraiana Rw2G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 425
AciI CCGC 6 cut(s) 65, 140, 175, 258, 529, 583
AclI AACGTT 1 cut(s) 746
AclWI GGATC 2 cut(s) 26, 39
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 604
AcyI GRCGYC 2 cut(s) 255, 285
AfaI GTAC 2 cut(s) 427, 704
AfiI CCNNNNNNNGG 2 cut(s) 127, 275
AflII CTTAAG 1 cut(s) 706
AflIII ACRYGT 1 cut(s) 760
AgeI ACCGGT 1 cut(s) 422
AgsI TTSAA 7 cut(s) 385, 473, 619, 663, 809, 866, 894
Alw21I GWGCWC 4 cut(s) 51, 441, 486, 513
Alw26I GTCTC 1 cut(s) 302
AlwI GGATC 2 cut(s) 26, 39
AlwNI CAGNNNCTG 1 cut(s) 571
Ama87I CYCGRG 2 cut(s) 299, 506
AoxI GGCC 2 cut(s) 3, 736
ApoI RAATTY 1 cut(s) 604
AsiGI ACCGGT 1 cut(s) 422
Asp718I GGTACC 1 cut(s) 425
AspS9I GGNCC 1 cut(s) 88
AsuHPI GGTGA 5 cut(s) 91, 172, 412, 544, 613
AsuII TTCGAA 2 cut(s) 518, 725
AsuNHI GCTAGC 1 cut(s) 787
AvaI CYCGRG 2 cut(s) 299, 506
AvaII GGWCC 1 cut(s) 88
BamHI GGATCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 425
BanII GRGCYC 2 cut(s) 441, 513
Bbv12I GWGCWC 4 cut(s) 51, 441, 486, 513
BbvCI CCTCAGC 1 cut(s) 311
BccI CCATC 2 cut(s) 529, 920
BceAI ACGGC 2 cut(s) 78, 245
BcgI CGANNNNNNTGC 4 cut(s) 508, 542, 632, 666
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 2 cut(s) 692, 788
BfrI CTTAAG 1 cut(s) 706
BglII AGATCT 1 cut(s) 829
BisI GCNGC 1 cut(s) 258
BlpI GCTNAGC 1 cut(s) 635
BlsI GCNGC 1 cut(s) 259
Bme18I GGWCC 1 cut(s) 88
BmeT110I CYCGRG 2 cut(s) 299, 506
BmgT120I GGNCC 1 cut(s) 88
BmiI GGNNCC 2 cut(s) 33, 427
BmsI GCATC 2 cut(s) 633, 800
BmtI GCTAGC 1 cut(s) 791
BplI GAGNNNNNCTC 2 cut(s) 888, 920
Bpu10I CCTNAGC 1 cut(s) 311
Bpu1102I GCTNAGC 1 cut(s) 635
Bpu14I TTCGAA 2 cut(s) 518, 725
BpuEI CTTGAG 2 cut(s) 416, 985
Bsa29I ATCGAT 2 cut(s) 249, 523
BsaAI YACGTR 2 cut(s) 445, 761
BsaBI GATNNNNATC 1 cut(s) 36
BsaHI GRCGYC 2 cut(s) 255, 285
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 2 cut(s) 414, 876
BsaWI WCCGGW 2 cut(s) 293, 422
BsaXI ACNNNNNCTCC 2 cut(s) 275, 305
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 275
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 36
BseCI ATCGAT 2 cut(s) 249, 523
BseDI CCNNGG 2 cut(s) 414, 876
BseGI GGATG 2 cut(s) 76, 775
BseJI GATNNNNATC 1 cut(s) 36
BseLI CCNNNNNNNGG 2 cut(s) 127, 275
BseMII CTCAG 3 cut(s) 144, 302, 626
BseNI ACTGG 1 cut(s) 102
BseRI GAGGAG 2 cut(s) 692, 710
BseYI CCCAGC 2 cut(s) 279, 577
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 2 cut(s) 5, 738
BshNI GGYRCC 1 cut(s) 425
BshTI ACCGGT 1 cut(s) 422
BshVI ATCGAT 2 cut(s) 249, 523
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 4 cut(s) 51, 441, 486, 513
BsiHKCI CYCGRG 2 cut(s) 299, 506
BsiSI CCGG 4 cut(s) 29, 276, 294, 423
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 127, 275
BsmAI GTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 2 cut(s) 5, 738
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 2 cut(s) 299, 506
Bsp119I TTCGAA 2 cut(s) 518, 725
Bsp1286I GDGCHC 4 cut(s) 51, 441, 486, 513
Bsp143I GATC 3 cut(s) 31, 55, 829
Bsp1720I GCTNAGC 1 cut(s) 635
BspACI CCGC 6 cut(s) 65, 140, 175, 258, 529, 583
BspANI GGCC 2 cut(s) 5, 738
BspCNI CTCAG 3 cut(s) 145, 303, 627
BspDI ATCGAT 2 cut(s) 249, 523
BspLI GGNNCC 2 cut(s) 33, 427
BspOI GCTAGC 1 cut(s) 791
BspPI GGATC 2 cut(s) 26, 39
BspQI GCTCTTC 1 cut(s) 39
BspT104I TTCGAA 2 cut(s) 518, 725
BspT107I GGYRCC 1 cut(s) 425
BspTI CTTAAG 1 cut(s) 706
BspTNI GGTCTC 1 cut(s) 302
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 1 cut(s) 102
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 2 cut(s) 414, 876
BssMI GATC 3 cut(s) 31, 55, 829
BssNI GRCGYC 2 cut(s) 255, 285
BssT1I CCWWGG 2 cut(s) 414, 876
Bst4CI ACNGT 2 cut(s) 88, 820
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 2 cut(s) 255, 285
BstAFI CTTAAG 1 cut(s) 706
BstBAI YACGTR 2 cut(s) 445, 761
BstBI TTCGAA 2 cut(s) 518, 725
BstC8I GCNNGC 2 cut(s) 641, 789
BstDEI CTNAG 4 cut(s) 153, 187, 311, 635
BstEII GGTNACC 1 cut(s) 418
BstF5I GGATG 2 cut(s) 76, 775
BstKTI GATC 3 cut(s) 34, 58, 832
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 3 cut(s) 31, 55, 829
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 3 cut(s) 183, 640, 645
BstPI GGTNACC 1 cut(s) 418
BstSNI TACGTA 1 cut(s) 445
BstX2I RGATCY 2 cut(s) 31, 829
BstYI RGATCY 2 cut(s) 31, 829
Bsu15I ATCGAT 2 cut(s) 249, 523
BsuRI GGCC 2 cut(s) 5, 738
BsuTUI ATCGAT 2 cut(s) 249, 523
BtsCI GGATG 2 cut(s) 76, 775
BtsI GCAGTG 1 cut(s) 23
BtsIMutI CAGTG 2 cut(s) 23, 825
Cac8I GCNNGC 2 cut(s) 641, 789
CaiI CAGNNNCTG 1 cut(s) 571
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 88
ClaI ATCGAT 2 cut(s) 249, 523
CseI GACGC 2 cut(s) 263, 293
Csp6I GTAC 2 cut(s) 426, 703
CspAI ACCGGT 1 cut(s) 422
CviAII CATG 6 cut(s) 116, 404, 491, 553, 933, 1004
CviQI GTAC 2 cut(s) 426, 703
DdeI CTNAG 4 cut(s) 153, 187, 311, 635
DpnI GATC 3 cut(s) 33, 57, 831
DpnII GATC 3 cut(s) 31, 55, 829
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EciI GGCGGA 2 cut(s) 80, 598
Ecl136II GAGCTC 2 cut(s) 439, 511
Eco105I TACGTA 1 cut(s) 445
Eco130I CCWWGG 2 cut(s) 414, 876
Eco147I AGGCCT 1 cut(s) 738
Eco24I GRGCYC 2 cut(s) 441, 513
Eco31I GGTCTC 1 cut(s) 302
Eco32I GATATC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 88
Eco53kI GAGCTC 2 cut(s) 439, 511
Eco88I CYCGRG 2 cut(s) 299, 506
Eco91I GGTNACC 1 cut(s) 418
EcoICRI GAGCTC 2 cut(s) 439, 511
EcoO65I GGTNACC 1 cut(s) 418
EcoRV GATATC 1 cut(s) 273
EcoT14I CCWWGG 2 cut(s) 414, 876
EcoT22I ATGCAT 1 cut(s) 626
EcoT38I GRGCYC 2 cut(s) 441, 513
ErhI CCWWGG 2 cut(s) 414, 876
FaeI CATG 6 cut(s) 119, 407, 494, 556, 936, 1007
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FaqI GGGAC 1 cut(s) 296
FatI CATG 6 cut(s) 115, 403, 490, 552, 932, 1003
FauI CCCGC 1 cut(s) 522
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 2 cut(s) 83, 782
FriOI GRGCYC 2 cut(s) 441, 513
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 2 cut(s) 692, 788
GluI GCNGC 1 cut(s) 258
GsaI CCCAGC 2 cut(s) 283, 581
HaeIII GGCC 2 cut(s) 5, 738
HapII CCGG 4 cut(s) 29, 276, 294, 423
HgaI GACGC 2 cut(s) 263, 293
Hin1I GRCGYC 2 cut(s) 255, 285
Hin1II CATG 6 cut(s) 119, 407, 494, 556, 936, 1007
HincII GTYRAC 2 cut(s) 657, 754
HindII GTYRAC 2 cut(s) 657, 754
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 9 cut(s) 37, 74, 125, 145, 502, 520, 586, 857, 998
HpaII CCGG 4 cut(s) 29, 276, 294, 423
HphI GGTGA 5 cut(s) 91, 172, 412, 544, 613
Hpy166II GTNNAC 2 cut(s) 657, 754
Hpy188I TCNGA 6 cut(s) 36, 60, 198, 240, 501, 591
Hpy188III TCNNGA 8 cut(s) 41, 299, 355, 506, 628, 692, 894, 920
Hpy8I GTNNAC 2 cut(s) 657, 754
Hpy99I CGWCG 4 cut(s) 56, 88, 266, 400
HpyAV CCTTC 2 cut(s) 728, 860
HpyCH4III ACNGT 2 cut(s) 88, 820
HpyCH4IV ACGT 4 cut(s) 395, 444, 746, 760
HpyCH4V TGCA 6 cut(s) 170, 547, 565, 624, 643, 938
HpyF10VI GCNNNNNNNGC 3 cut(s) 183, 640, 645
HpyF3I CTNAG 4 cut(s) 153, 187, 311, 635
HpySE526I ACGT 4 cut(s) 395, 444, 746, 760
Hsp92I GRCGYC 2 cut(s) 255, 285
Hsp92II CATG 6 cut(s) 119, 407, 494, 556, 936, 1007
KpnI GGTACC 1 cut(s) 429
Kzo9I GATC 3 cut(s) 31, 55, 829
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 117, 436, 568
LweI GCATC 2 cut(s) 633, 800
MaeI CTAG 2 cut(s) 692, 788
MaeII ACGT 4 cut(s) 395, 444, 746, 760
MaeIII GTNAC 6 cut(s) 135, 228, 399, 418, 460, 858
MalI GATC 3 cut(s) 33, 57, 831
MboI GATC 3 cut(s) 31, 55, 829
MboII GAAGA 5 cut(s) 56, 69, 485, 839, 967
MflI RGATCY 2 cut(s) 31, 829
MhlI GDGCHC 4 cut(s) 51, 441, 486, 513
MluCI AATT 3 cut(s) 477, 604, 993
MlyI GAGTC 3 cut(s) 119, 154, 866
MmeI TCCRAC 1 cut(s) 237
Mph1103I ATGCAT 1 cut(s) 626
MseI TTAA 4 cut(s) 594, 707, 840, 1015
MslI CAYNNNNRTG 1 cut(s) 489
MspCI CTTAAG 1 cut(s) 706
MspI CCGG 4 cut(s) 29, 276, 294, 423
MwoI GCNNNNNNNGC 3 cut(s) 183, 640, 645
NdeII GATC 3 cut(s) 31, 55, 829
NheI GCTAGC 1 cut(s) 787
NlaIII CATG 6 cut(s) 119, 407, 494, 556, 936, 1007
NlaIV GGNNCC 2 cut(s) 33, 427
NmeAIII GCCGAG 1 cut(s) 168
NmuCI GTSAC 3 cut(s) 228, 418, 858
NsiI ATGCAT 1 cut(s) 626
NspV TTCGAA 2 cut(s) 518, 725
PaeR7I CTCGAG 2 cut(s) 299, 506
PceI AGGCCT 1 cut(s) 738
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 261
PfeI GAWTC 6 cut(s) 37, 74, 502, 520, 586, 998
PflFI GACNNNGTC 1 cut(s) 128
PinAI ACCGGT 1 cut(s) 422
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 3 cut(s) 119, 153, 865
PpsI GAGTC 3 cut(s) 119, 153, 865
Ppu21I YACGTR 2 cut(s) 445, 761
Psp124BI GAGCTC 2 cut(s) 441, 513
Psp1406I AACGTT 1 cut(s) 746
PspEI GGTNACC 1 cut(s) 418
PspFI CCCAGC 2 cut(s) 279, 577
PspN4I GGNNCC 2 cut(s) 33, 427
PspPI GGNCC 1 cut(s) 88
PstNI CAGNNNCTG 1 cut(s) 571
PsuI RGATCY 2 cut(s) 31, 829
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 2 cut(s) 427, 704
RsaNI GTAC 2 cut(s) 426, 703
RseI CAYNNNNRTG 1 cut(s) 489
SacI GAGCTC 2 cut(s) 441, 513
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 4 cut(s) 594, 707, 840, 1015
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 3 cut(s) 31, 55, 829
Sau96I GGNCC 1 cut(s) 88
SchI GAGTC 3 cut(s) 119, 154, 866
SduI GDGCHC 4 cut(s) 51, 441, 486, 513
SfaNI GCATC 2 cut(s) 633, 800
Sfr274I CTCGAG 2 cut(s) 299, 506
SfuI TTCGAA 2 cut(s) 518, 725
SinI GGWCC 1 cut(s) 88
SlaI CTCGAG 2 cut(s) 299, 506
SmiMI CAYNNNNRTG 1 cut(s) 489
SmlI CTYRAG 5 cut(s) 299, 431, 506, 706, 964
SmoI CTYRAG 5 cut(s) 299, 431, 506, 706, 964
SnaBI TACGTA 1 cut(s) 445
Sse9I AATT 3 cut(s) 477, 604, 993
SseBI AGGCCT 1 cut(s) 738
SsiI CCGC 6 cut(s) 65, 140, 175, 258, 529, 583
SspMI CTAG 2 cut(s) 692, 788
SstI GAGCTC 2 cut(s) 441, 513
StuI AGGCCT 1 cut(s) 738
StyI CCWWGG 2 cut(s) 414, 876
TaaI ACNGT 2 cut(s) 88, 820
TaiI ACGT 4 cut(s) 398, 447, 749, 763
TaqII GACCGA 1 cut(s) 118
TasI AATT 3 cut(s) 477, 604, 993
TatI WGTACW 1 cut(s) 702
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 6 cut(s) 37, 74, 502, 520, 586, 998
Tru1I TTAA 4 cut(s) 594, 707, 840, 1015
Tru9I TTAA 4 cut(s) 594, 707, 840, 1015
TscAI CASTG 2 cut(s) 30, 825
TseFI GTSAC 3 cut(s) 228, 418, 858
Tsp45I GTSAC 3 cut(s) 228, 418, 858
TspDTI ATGAA 2 cut(s) 471, 507
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 2 cut(s) 30, 825
Tth111I GACNNNGTC 1 cut(s) 128
Vha464I CTTAAG 1 cut(s) 706
VpaK11BI GGWCC 1 cut(s) 88
XapI RAATTY 1 cut(s) 604
XbaI TCTAGA 1 cut(s) 691
XhoI CTCGAG 2 cut(s) 299, 506
XspI CTAG 2 cut(s) 692, 788
Zsp2I ATGCAT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.