RLG00000016234

Protein phosphatase 1 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011350
Physical Location & Seq
Reverse (-)
4934180 .. 4936409
2230 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016234

Sequence Viewer

Length: 1050 bp
ATGGACGACCAGGCCCAACCAGCGCCGAATCACGATGATCCGACGGCCGAGAACGACGGCGATCCTTCCTCCACCGTCCTCGATCTCACAAGCTTCCAACTCCACGACCTCCACTCGGTCGAGTTACCGCCGAGTCTCACCGAGTTAGACCTCACCGCCAACCGCCTCTCCTCCTTGGACACTCGCATTGGAACCCTCTCCAACCTCACCAAGCTCTCCTTCCGCCAGAACCTCATCGACGACGCCGCCGTCCAACCGATCTCCTCCTGGACCGCCCTCTCGGGCCTCGAGGAGTTGGTGCTGAGGGATAATAAGCTGAATAAAATACCTGATGTGAGCATCTTCAAGAAGCTTCTGGTTTTCGACGTCTCTTTCAATGAGATAACATCGTTGCAGGGGCTTTCCAAGGTCTCCAGCACCCTCAAGGAGCTCTATGTCTCCAAAAATGAAGTTACTAAGATTGAGGAGATTGATCATCTCCATCAGCTTCAAATTCTTGAGCTAGGTTCGAACCGGTTAAGGGTGATGCAGAGTCTGGAAAATATGACAAAGTTAGAAGAGCTGTGGCTGGGACGGAATCGTATAAAGGTAGTTAATTTATGTGGGCTGAAATGCATTAAGAAGATTAGCTTGCAAAGCAATCGGTTAACTTCTATGACTGGATTTGAGGAATGTGTTGCGCTTGAGGAACTGTACTTGAGCCATAATGGGATTTCCAAAATGGAAGGCCTCTCAACCTTGGTTAACCTGCGTGTGTTGGATGTATCTAATAACAAGCTAACATCAGTGAATGATATCGAAAACCTAACAATGTTAGAAGACCTGTGGCTTAATGACAACCAGATAGAGTCAATTGAAGGCGTTGCTGAGGCTGTTGTTGGTTCGAGAGAGAAGCTGGCTACTATCTATCTCGAGCACAATCCATGTGCAAAAAATCCAAACTATTCCGCTGCACTGGAGATCTATAGCTGCAATACAGGCCAATGTCCAGGATTCTTGCTTCATTTGACTAATTCGATGGATGACCAGTACCAAGAGGATATGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

350

Amino Acids

39.03

Weight (kDa)

4.7

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 228 - 268 2.8e-09 Leucine Rich repeats (2 copies)
LRR_8 PF13855 228 - 282 1e-05 Leucine rich repeat
LRR_4 PF12799 249 - 288 3.4e-08 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19680
fragaria_vesca FvH4_1g05880 FvH4_1g05880
malus_domestica MD02G1060900.v1.1 MD15G1194400.v1.1
prunus_persica Prupe.7G222100_v2.0.a1 Prupe.7G222100_v2.0.a1
pyrus_communis pycom02g05000 pycom15g17400
rosa_chinensis RchiOBHm_Chr2g0091541 RchiOBHm_Chr2g0091621
rosa_laevigata RLG00000016234
rosa_multiflora Rmu_sc0002407.1_g000003
rosa_roxburghii Rroxscaffold_2G00149950
rosa_rugosa Rorug02G0018600
rosa_samantha Rh2AG063800 Rh2BG063200 Rh2CG064900 Rh2DG063300
rosa_wichuraiana Rw2G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AatII GACGTC 1 cut(s) 369
AbsI CCTCGAGG 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 756
AciI CCGC 7 cut(s) 128, 156, 163, 223, 246, 273, 948
AclWI GGATC 2 cut(s) 32, 56
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 492
AcyI GRCGYC 2 cut(s) 243, 366
AfaI GTAC 2 cut(s) 695, 1031
AfiI CCNNNNNNNGG 2 cut(s) 115, 520
AgeI ACCGGT 1 cut(s) 513
AgsI TTSAA 4 cut(s) 346, 376, 491, 857
AjnI CCWGG 3 cut(s) 9, 266, 988
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
Alw21I GWGCWC 2 cut(s) 432, 918
Alw26I GTCTC 4 cut(s) 140, 373, 415, 442
AlwI GGATC 2 cut(s) 32, 56
AlwNI CAGNNNCTG 1 cut(s) 535
Ama87I CYCGRG 3 cut(s) 280, 287, 911
AoxI GGCC 5 cut(s) 12, 45, 283, 727, 979
ApeKI GCWGC 2 cut(s) 950, 969
ApoI RAATTY 1 cut(s) 492
AsiGI ACCGGT 1 cut(s) 513
AspLEI GCGC 2 cut(s) 25, 682
AspS9I GGNCC 3 cut(s) 13, 270, 283
AsuHPI GGTGA 4 cut(s) 130, 145, 199, 535
AsuII TTCGAA 1 cut(s) 509
AvaI CYCGRG 3 cut(s) 280, 287, 911
AvaII GGWCC 1 cut(s) 270
BanII GRGCYC 1 cut(s) 432
BbsI GAAGAC 1 cut(s) 825
Bbv12I GWGCWC 2 cut(s) 432, 918
BbvCI CCTCAGC 2 cut(s) 302, 867
BbvI GCAGC 2 cut(s) 937, 956
BccI CCATC 2 cut(s) 489, 1012
BceAI ACGGC 3 cut(s) 60, 73, 233
BcgI CGANNNNNNTGC 2 cut(s) 623, 657
BciT130I CCWGG 3 cut(s) 11, 268, 990
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 4 cut(s) 140, 373, 415, 442
BfaI CTAG 2 cut(s) 503, 1048
BfmI CTRYAG 1 cut(s) 964
BfoI RGCGCY 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 756
BglII AGATCT 1 cut(s) 960
BisI GCNGC 3 cut(s) 246, 951, 970
BlsI GCNGC 3 cut(s) 247, 952, 971
Bme1390I CCNGG 3 cut(s) 11, 268, 990
Bme18I GGWCC 1 cut(s) 270
BmeT110I CYCGRG 3 cut(s) 280, 287, 911
BmgT120I GGNCC 3 cut(s) 13, 270, 283
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 3 cut(s) 11, 268, 990
BmsI GCATC 2 cut(s) 348, 516
BpiI GAAGAC 1 cut(s) 825
BpmI CTGGAG 2 cut(s) 397, 977
Bpu10I CCTNAGC 2 cut(s) 302, 867
Bpu14I TTCGAA 1 cut(s) 509
BpuEI CTTGAG 4 cut(s) 407, 518, 704, 718
BsaHI GRCGYC 2 cut(s) 243, 366
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 3 cut(s) 174, 405, 738
BsaWI WCCGGW 1 cut(s) 513
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 520
Bse118I RCCGGY 1 cut(s) 513
Bse1I ACTGG 3 cut(s) 664, 960, 1027
BseBI CCWGG 3 cut(s) 11, 268, 990
BseDI CCNNGG 3 cut(s) 174, 405, 738
BseGI GGATG 2 cut(s) 766, 1027
BseLI CCNNNNNNNGG 2 cut(s) 115, 520
BseMII CTCAG 2 cut(s) 293, 858
BseNI ACTGG 3 cut(s) 664, 960, 1027
BseRI GAGGAG 4 cut(s) 160, 253, 305, 479
BseX3I CGGCCG 1 cut(s) 45
BseXI GCAGC 2 cut(s) 937, 956
BseYI CCCAGC 1 cut(s) 568
BsgI GTGCAG 1 cut(s) 936
Bsh1285I CGRYCG 2 cut(s) 48, 120
BshFI GGCC 5 cut(s) 14, 47, 285, 729, 981
BshTI ACCGGT 1 cut(s) 513
BsiEI CGRYCG 2 cut(s) 48, 120
BsiHKAI GWGCWC 2 cut(s) 432, 918
BsiHKCI CYCGRG 3 cut(s) 280, 287, 911
BsiSI CCGG 1 cut(s) 514
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 2 cut(s) 115, 520
BsmAI GTCTC 4 cut(s) 140, 373, 415, 442
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 1 cut(s) 585
BsnI GGCC 5 cut(s) 14, 47, 285, 729, 981
Bso31I GGTCTC 1 cut(s) 415
BsoBI CYCGRG 3 cut(s) 280, 287, 911
Bsp119I TTCGAA 1 cut(s) 509
Bsp1286I GDGCHC 2 cut(s) 432, 918
Bsp143I GATC 6 cut(s) 37, 61, 82, 258, 472, 960
BspACI CCGC 7 cut(s) 128, 156, 163, 223, 246, 273, 948
BspANI GGCC 5 cut(s) 14, 47, 285, 729, 981
BspCNI CTCAG 2 cut(s) 294, 859
BspLI GGNNCC 1 cut(s) 193
BspMI ACCTGC 1 cut(s) 756
BspPI GGATC 2 cut(s) 32, 56
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 415
BsrFI RCCGGY 1 cut(s) 513
BsrI ACTGG 3 cut(s) 664, 960, 1027
BssAI RCCGGY 1 cut(s) 513
BssECI CCNNGG 3 cut(s) 174, 405, 738
BssMI GATC 6 cut(s) 37, 61, 82, 258, 472, 960
BssNI GRCGYC 2 cut(s) 243, 366
BssT1I CCWWGG 3 cut(s) 174, 405, 738
Bst2UI CCWGG 3 cut(s) 11, 268, 990
Bst4CI ACNGT 2 cut(s) 76, 693
Bst6I CTCTTC 1 cut(s) 552
BstACI GRCGYC 2 cut(s) 243, 366
BstBI TTCGAA 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 632, 897
BstDEI CTNAG 3 cut(s) 302, 456, 867
BstF5I GGATG 2 cut(s) 766, 1027
BstH2I RGCGCY 1 cut(s) 26
BstHHI GCGC 2 cut(s) 25, 682
BstKTI GATC 6 cut(s) 40, 64, 85, 261, 475, 963
BstMAI GTCTC 4 cut(s) 140, 373, 415, 442
BstMBI GATC 6 cut(s) 37, 61, 82, 258, 472, 960
BstMCI CGRYCG 2 cut(s) 48, 120
BstMWI GCNNNNNNNGC 3 cut(s) 20, 636, 978
BstNI CCWGG 3 cut(s) 11, 268, 990
BstSCI CCNGG 3 cut(s) 9, 266, 988
BstSFI CTRYAG 1 cut(s) 964
BstV1I GCAGC 2 cut(s) 937, 956
BstV2I GAAGAC 1 cut(s) 825
BstX2I RGATCY 1 cut(s) 960
BstYI RGATCY 1 cut(s) 960
BstZI CGGCCG 1 cut(s) 45
BsuRI GGCC 5 cut(s) 14, 47, 285, 729, 981
BtsCI GGATG 2 cut(s) 766, 1027
BtsIMutI CAGTG 2 cut(s) 792, 953
BveI ACCTGC 1 cut(s) 756
Cac8I GCNNGC 2 cut(s) 632, 897
CaiI CAGNNNCTG 1 cut(s) 535
CfoI GCGC 2 cut(s) 25, 682
Cfr10I RCCGGY 1 cut(s) 513
Cfr13I GGNCC 3 cut(s) 13, 270, 283
CseI GACGC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 694, 1030
CspAI ACCGGT 1 cut(s) 513
CviAII CATG 1 cut(s) 924
CviQI GTAC 2 cut(s) 694, 1030
DdeI CTNAG 3 cut(s) 302, 456, 867
DpnI GATC 6 cut(s) 39, 63, 84, 260, 474, 962
DpnII GATC 6 cut(s) 37, 61, 82, 258, 472, 960
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 45
EagI CGGCCG 1 cut(s) 45
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 1 cut(s) 212
Ecl136II GAGCTC 1 cut(s) 430
EclXI CGGCCG 1 cut(s) 45
Eco130I CCWWGG 3 cut(s) 174, 405, 738
Eco147I AGGCCT 1 cut(s) 729
Eco24I GRGCYC 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 415
Eco32I GATATC 1 cut(s) 796
Eco47I GGWCC 1 cut(s) 270
Eco52I CGGCCG 1 cut(s) 45
Eco53kI GAGCTC 1 cut(s) 430
Eco88I CYCGRG 3 cut(s) 280, 287, 911
EcoICRI GAGCTC 1 cut(s) 430
EcoRII CCWGG 3 cut(s) 9, 266, 988
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 3 cut(s) 174, 405, 738
EcoT22I ATGCAT 1 cut(s) 617
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 3 cut(s) 174, 405, 738
Esp3I CGTCTC 1 cut(s) 373
FaeI CATG 1 cut(s) 927
FaiI YATR 9 cut(s) 435, 545, 584, 601, 656, 705, 925, 966, 1043
FalI AAGNNNNNCTT 4 cut(s) 203, 235, 614, 646
FaqI GGGAC 1 cut(s) 585
FatI CATG 1 cut(s) 923
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 3 cut(s) 246, 951, 970
FokI GGATG 2 cut(s) 773, 1034
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 3 cut(s) 246, 951, 970
FspBI CTAG 2 cut(s) 503, 1048
GlaI GCGC 2 cut(s) 24, 681
GluI GCNGC 3 cut(s) 246, 951, 970
GsaI CCCAGC 1 cut(s) 572
GsuI CTGGAG 2 cut(s) 397, 977
HaeII RGCGCY 1 cut(s) 26
HaeIII GGCC 5 cut(s) 14, 47, 285, 729, 981
HapII CCGG 1 cut(s) 514
HgaI GACGC 1 cut(s) 251
HhaI GCGC 2 cut(s) 25, 682
Hin1I GRCGYC 2 cut(s) 243, 366
Hin1II CATG 1 cut(s) 927
Hin6I GCGC 2 cut(s) 23, 680
HinP1I GCGC 2 cut(s) 23, 680
HincII GTYRAC 2 cut(s) 648, 745
HindII GTYRAC 2 cut(s) 648, 745
HindIII AAGCTT 2 cut(s) 91, 350
HinfI GANTC 6 cut(s) 28, 133, 532, 577, 848, 993
HpaI GTTAAC 2 cut(s) 648, 745
HpaII CCGG 1 cut(s) 514
HphI GGTGA 4 cut(s) 130, 145, 199, 535
Hpy166II GTNNAC 2 cut(s) 648, 745
Hpy188I TCNGA 1 cut(s) 42
Hpy188III TCNNGA 6 cut(s) 32, 346, 497, 536, 885, 911
Hpy8I GTNNAC 2 cut(s) 648, 745
Hpy99I CGWCG 5 cut(s) 46, 59, 242, 245, 368
HpyAV CCTTC 4 cut(s) 75, 229, 719, 851
HpyCH4III ACNGT 2 cut(s) 76, 693
HpyCH4IV ACGT 1 cut(s) 366
HpyCH4V TGCA 7 cut(s) 394, 529, 615, 634, 929, 953, 972
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 636, 978
HpyF3I CTNAG 3 cut(s) 302, 456, 867
HpySE526I ACGT 1 cut(s) 366
Hsp92I GRCGYC 2 cut(s) 243, 366
Hsp92II CATG 1 cut(s) 927
HspAI GCGC 2 cut(s) 23, 680
Ksp22I TGATCA 1 cut(s) 472
KspAI GTTAAC 2 cut(s) 648, 745
Kzo9I GATC 6 cut(s) 37, 61, 82, 258, 472, 960
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 1 cut(s) 427
Lsp1109I GCAGC 2 cut(s) 937, 956
LweI GCATC 2 cut(s) 348, 516
MaeI CTAG 2 cut(s) 503, 1048
MaeII ACGT 1 cut(s) 366
MaeIII GTNAC 2 cut(s) 123, 451
MalI GATC 6 cut(s) 39, 63, 84, 260, 474, 962
MboI GATC 6 cut(s) 37, 61, 82, 258, 472, 960
MboII GAAGA 4 cut(s) 334, 569, 634, 830
MfeI CAATTG 1 cut(s) 852
MflI RGATCY 1 cut(s) 960
MhlI GDGCHC 2 cut(s) 432, 918
MluCI AATT 4 cut(s) 492, 595, 852, 1012
MlyI GAGTC 3 cut(s) 142, 541, 857
MmeI TCCRAC 5 cut(s) 65, 121, 225, 277, 738
Mph1103I ATGCAT 1 cut(s) 617
MseI TTAA 6 cut(s) 518, 594, 618, 647, 744, 831
MspA1I CMGCKG 1 cut(s) 950
MspI CCGG 1 cut(s) 514
MspR9I CCNGG 3 cut(s) 11, 268, 990
MunI CAATTG 1 cut(s) 852
MvaI CCWGG 3 cut(s) 11, 268, 990
MwoI GCNNNNNNNGC 3 cut(s) 20, 636, 978
NdeII GATC 6 cut(s) 37, 61, 82, 258, 472, 960
NlaIII CATG 1 cut(s) 927
NlaIV GGNNCC 1 cut(s) 193
NmeAIII GCCGAG 2 cut(s) 73, 156
NsiI ATGCAT 1 cut(s) 617
NspV TTCGAA 1 cut(s) 509
PaeR7I CTCGAG 2 cut(s) 287, 911
PceI AGGCCT 1 cut(s) 729
PciSI GCTCTTC 1 cut(s) 552
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PfeI GAWTC 3 cut(s) 28, 577, 993
PfoI TCCNGGA 2 cut(s) 266, 988
PinAI ACCGGT 1 cut(s) 513
PkrI GCNGC 3 cut(s) 247, 952, 971
PleI GAGTC 3 cut(s) 141, 540, 856
PpsI GAGTC 3 cut(s) 141, 540, 856
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 3 cut(s) 9, 266, 988
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 3 cut(s) 9, 266, 988
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 3 cut(s) 13, 270, 283
PspXI VCTCGAGB 1 cut(s) 287
PstNI CAGNNNCTG 1 cut(s) 535
PsuI RGATCY 1 cut(s) 960
RsaI GTAC 2 cut(s) 695, 1031
RsaNI GTAC 2 cut(s) 694, 1030
SacI GAGCTC 1 cut(s) 432
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 6 cut(s) 518, 594, 618, 647, 744, 831
SatI GCNGC 3 cut(s) 246, 951, 970
Sau3AI GATC 6 cut(s) 37, 61, 82, 258, 472, 960
Sau96I GGNCC 3 cut(s) 13, 270, 283
SchI GAGTC 3 cut(s) 142, 541, 857
ScrFI CCNGG 3 cut(s) 11, 268, 990
SduI GDGCHC 2 cut(s) 432, 918
SfaNI GCATC 2 cut(s) 348, 516
SfcI CTRYAG 1 cut(s) 964
Sfr274I CTCGAG 2 cut(s) 287, 911
SfuI TTCGAA 1 cut(s) 509
SinI GGWCC 1 cut(s) 270
SlaI CTCGAG 2 cut(s) 287, 911
SmlI CTYRAG 6 cut(s) 287, 422, 497, 683, 697, 911
SmoI CTYRAG 6 cut(s) 287, 422, 497, 683, 697, 911
Sse9I AATT 4 cut(s) 492, 595, 852, 1012
SseBI AGGCCT 1 cut(s) 729
SsiI CCGC 7 cut(s) 128, 156, 163, 223, 246, 273, 948
SspMI CTAG 2 cut(s) 503, 1048
SstI GAGCTC 1 cut(s) 432
StuI AGGCCT 1 cut(s) 729
StyD4I CCNGG 3 cut(s) 9, 266, 988
StyI CCWWGG 3 cut(s) 174, 405, 738
TaaI ACNGT 2 cut(s) 76, 693
TaiI ACGT 1 cut(s) 369
TaqII GACCGA 1 cut(s) 106
TasI AATT 4 cut(s) 492, 595, 852, 1012
TatI WGTACW 1 cut(s) 693
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 3 cut(s) 28, 577, 993
Tru1I TTAA 6 cut(s) 518, 594, 618, 647, 744, 831
Tru9I TTAA 6 cut(s) 518, 594, 618, 647, 744, 831
TscAI CASTG 2 cut(s) 792, 960
TseI GCWGC 2 cut(s) 950, 969
TspDTI ATGAA 2 cut(s) 462, 992
TspGWI ACGGA 1 cut(s) 589
TspRI CASTG 2 cut(s) 792, 960
VpaK11BI GGWCC 1 cut(s) 270
XapI RAATTY 1 cut(s) 492
XhoI CTCGAG 2 cut(s) 287, 911
XspI CTAG 2 cut(s) 503, 1048
ZraI GACGTC 1 cut(s) 367
Zsp2I ATGCAT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.