Rroxscaffold_2G00149950

Protein phosphatase 1 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011350
Physical Location & Seq
Forward (+)
87451005 .. 87453411
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149950.1

Sequence Viewer

Length: 996 bp
ATGGACGACCAGGCCCAACCACCACCGCCGCCGAATCACGATGATCCAACGGCCGAGAACGACGGCGATCCTTCCTCCACCGTCCTCGATCTCACCAGCTTCCAACTCCACGACCTCCACTCGGTCGAGTTACCGCCGAGTCTCACTGAGTTAGACCTCACCGCCAACCGCCTCTCCTCCTTGGACACTCGCATTGGAACCCTCTCCAACCTCACCAAGCTCTCCTTCCGCCAGAACCTCATCGACGACGCCGCCGTCCAACCGATCTCCGACTGGACCGCCCTCTCCGGCCTCGAGGAGTTGGTGCTGAGGGATAATAAGCTGAACAAAATACCTGATGTGAGCATCTTCAAGAAGCTTCTGGTTTTCGACGTCTCTTTCAATGAGATTACATCGTTGCAGGGGCTTTCCACGGTCTCCAGCACCCTCAAGGAGCTCTATGTCTCCAAAAATGAAGTTACTAAGATTGAGGAGATTGATCATCTCCATCAGCTTCAAATTCTTGAGCTAGGTTCGAACCGGTTAAGGGTGATGCAGAGTCTGGAAAATATGACAAAGTTAGAAGAGCTGTGGCTGGGACGGAATCGTATAAAGGTAGTTAATTTATGTGGGCTGAAATGCATTAAGAAGATTAGCTTGCAAAGCAATCGGTTAACTTCTATGACTGGATTTGAGGAATGTGTTTCGCTTGAGGAACTGTACTTGAGCCATAATGGGATTTCCAAAATGGAAGGCCTCTCAACCTTGGTTAACCTGCGTGTGTTGGATGTATCTAATAACAAGCTAACATCAGTGAATGATATCGAAAACCTAACAATGTTAGAAGACCTGTGGCTTAATGACAACCAGATAGAGTCAATTGAAGGCATTGCTGAGGCTGTTGTTGGTTCGAGAGAGAAGCTGGCTACTATCTATCTCGAACACAATCCATGTGTAAAAAAATCCAAACTATTCCGCTGCATTGAAAAAAACCTTCCCAAACATACAGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

331

Amino Acids

37.14

Weight (kDa)

4.98

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 229 - 270 1.5e-09 Leucine Rich repeats (2 copies)
LRR_8 PF13855 229 - 284 6.4e-06 Leucine rich repeat
LRR_4 PF12799 251 - 291 2.9e-08 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19680
fragaria_vesca FvH4_1g05880 FvH4_1g05880
malus_domestica MD02G1060900.v1.1 MD15G1194400.v1.1
prunus_persica Prupe.7G222100_v2.0.a1 Prupe.7G222100_v2.0.a1
pyrus_communis pycom02g05000 pycom15g17400
rosa_chinensis RchiOBHm_Chr2g0091541 RchiOBHm_Chr2g0091621
rosa_laevigata RLG00000016234
rosa_multiflora Rmu_sc0002407.1_g000003
rosa_roxburghii Rroxscaffold_2G00149950
rosa_rugosa Rorug02G0018600
rosa_samantha Rh2AG063800 Rh2BG063200 Rh2CG064900 Rh2DG063300
rosa_wichuraiana Rw2G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
AatII GACGTC 1 cut(s) 375
AbsI CCTCGAGG 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 762
AciI CCGC 9 cut(s) 26, 29, 134, 162, 169, 229, 252, 279, 955
AclWI GGATC 2 cut(s) 38, 62
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 1 cut(s) 498
AcyI GRCGYC 2 cut(s) 249, 372
AfaI GTAC 1 cut(s) 701
AfiI CCNNNNNNNGG 2 cut(s) 121, 526
AgeI ACCGGT 1 cut(s) 519
AgsI TTSAA 5 cut(s) 352, 382, 497, 863, 965
AjnI CCWGG 1 cut(s) 9
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 4 cut(s) 146, 379, 421, 448
AlwI GGATC 2 cut(s) 38, 62
AlwNI CAGNNNCTG 1 cut(s) 541
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 4 cut(s) 12, 51, 289, 733
ApeKI GCWGC 1 cut(s) 957
ApoI RAATTY 1 cut(s) 498
AsiGI ACCGGT 1 cut(s) 519
AspS9I GGNCC 2 cut(s) 13, 276
AsuHPI GGTGA 4 cut(s) 85, 151, 205, 541
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 1 cut(s) 276
BanII GRGCYC 1 cut(s) 438
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 1 cut(s) 438
BbvCI CCTCAGC 2 cut(s) 308, 873
BbvI GCAGC 1 cut(s) 944
BccI CCATC 1 cut(s) 495
BceAI ACGGC 3 cut(s) 66, 79, 239
BcgI CGANNNNNNTGC 2 cut(s) 629, 663
BciT130I CCWGG 1 cut(s) 11
BclI TGATCA 1 cut(s) 478
BcoDI GTCTC 4 cut(s) 146, 379, 421, 448
BfaI CTAG 1 cut(s) 509
BfuAI ACCTGC 1 cut(s) 762
BisI GCNGC 3 cut(s) 29, 252, 958
BlsI GCNGC 3 cut(s) 30, 253, 959
Bme1390I CCNGG 1 cut(s) 11
Bme18I GGWCC 1 cut(s) 276
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 2 cut(s) 13, 276
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 1 cut(s) 11
BmsI GCATC 2 cut(s) 354, 522
BpiI GAAGAC 1 cut(s) 831
BpmI CTGGAG 1 cut(s) 403
Bpu10I CCTNAGC 2 cut(s) 308, 873
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 4 cut(s) 413, 524, 710, 724
BsaHI GRCGYC 2 cut(s) 249, 372
BsaI GGTCTC 1 cut(s) 421
BsaJI CCNNGG 3 cut(s) 180, 411, 744
BsaWI WCCGGW 1 cut(s) 519
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 526
Bse118I RCCGGY 1 cut(s) 519
Bse1I ACTGG 2 cut(s) 278, 670
Bse3DI GCAATG 1 cut(s) 867
BseBI CCWGG 1 cut(s) 11
BseDI CCNNGG 3 cut(s) 180, 411, 744
BseGI GGATG 1 cut(s) 772
BseLI CCNNNNNNNGG 2 cut(s) 121, 526
BseMI GCAATG 1 cut(s) 867
BseMII CTCAG 3 cut(s) 138, 299, 864
BseNI ACTGG 2 cut(s) 278, 670
BseRI GAGGAG 3 cut(s) 166, 311, 485
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 944
BseYI CCCAGC 1 cut(s) 574
Bsh1285I CGRYCG 2 cut(s) 54, 126
BshFI GGCC 4 cut(s) 14, 53, 291, 735
BshTI ACCGGT 1 cut(s) 519
BsiEI CGRYCG 2 cut(s) 54, 126
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 2 cut(s) 288, 520
BslFI GGGAC 1 cut(s) 591
BslI CCNNNNNNNGG 2 cut(s) 121, 526
BsmAI GTCTC 4 cut(s) 146, 379, 421, 448
BsmBI CGTCTC 1 cut(s) 379
BsmFI GGGAC 1 cut(s) 591
BsnI GGCC 4 cut(s) 14, 53, 291, 735
Bso31I GGTCTC 1 cut(s) 421
BsoBI CYCGRG 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 5 cut(s) 43, 67, 88, 264, 478
BspACI CCGC 9 cut(s) 26, 29, 134, 162, 169, 229, 252, 279, 955
BspANI GGCC 4 cut(s) 14, 53, 291, 735
BspCNI CTCAG 3 cut(s) 139, 300, 865
BspLI GGNNCC 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 762
BspPI GGATC 2 cut(s) 38, 62
BspQI GCTCTTC 1 cut(s) 558
BspT104I TTCGAA 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 421
BsrDI GCAATG 1 cut(s) 867
BsrFI RCCGGY 1 cut(s) 519
BsrI ACTGG 2 cut(s) 278, 670
BssAI RCCGGY 1 cut(s) 519
BssECI CCNNGG 3 cut(s) 180, 411, 744
BssMI GATC 5 cut(s) 43, 67, 88, 264, 478
BssNI GRCGYC 2 cut(s) 249, 372
BssT1I CCWWGG 2 cut(s) 180, 744
Bst2UI CCWGG 1 cut(s) 11
Bst4CI ACNGT 3 cut(s) 82, 415, 699
Bst6I CTCTTC 1 cut(s) 558
BstACI GRCGYC 2 cut(s) 249, 372
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 638, 903
BstDEI CTNAG 4 cut(s) 147, 308, 462, 873
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 1 cut(s) 772
BstKTI GATC 5 cut(s) 46, 70, 91, 267, 481
BstMAI GTCTC 4 cut(s) 146, 379, 421, 448
BstMBI GATC 5 cut(s) 43, 67, 88, 264, 478
BstMCI CGRYCG 2 cut(s) 54, 126
BstMWI GCNNNNNNNGC 1 cut(s) 642
BstNI CCWGG 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 944
BstV2I GAAGAC 1 cut(s) 831
BstZI CGGCCG 1 cut(s) 51
BsuRI GGCC 4 cut(s) 14, 53, 291, 735
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 1 cut(s) 772
BtsIMutI CAGTG 2 cut(s) 144, 798
BveI ACCTGC 1 cut(s) 762
Cac8I GCNNGC 2 cut(s) 638, 903
CaiI CAGNNNCTG 1 cut(s) 541
Cfr10I RCCGGY 1 cut(s) 519
Cfr13I GGNCC 2 cut(s) 13, 276
CseI GACGC 1 cut(s) 257
Csp6I GTAC 1 cut(s) 700
CspAI ACCGGT 1 cut(s) 519
CviAII CATG 1 cut(s) 930
CviQI GTAC 1 cut(s) 700
DdeI CTNAG 4 cut(s) 147, 308, 462, 873
DpnI GATC 5 cut(s) 45, 69, 90, 266, 480
DpnII GATC 5 cut(s) 43, 67, 88, 264, 478
DrdI GACNNNNNNGTC 1 cut(s) 254
DseDI GACNNNNNNGTC 1 cut(s) 254
EaeI YGGCCR 1 cut(s) 51
EagI CGGCCG 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
EciI GGCGGA 1 cut(s) 218
Ecl136II GAGCTC 1 cut(s) 436
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 2 cut(s) 180, 744
Eco147I AGGCCT 1 cut(s) 735
Eco24I GRGCYC 1 cut(s) 438
Eco31I GGTCTC 1 cut(s) 421
Eco32I GATATC 1 cut(s) 802
Eco47I GGWCC 1 cut(s) 276
Eco52I CGGCCG 1 cut(s) 51
Eco53kI GAGCTC 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 436
EcoRII CCWGG 1 cut(s) 9
EcoRV GATATC 1 cut(s) 802
EcoT14I CCWWGG 2 cut(s) 180, 744
EcoT22I ATGCAT 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 438
ErhI CCWWGG 2 cut(s) 180, 744
Esp3I CGTCTC 1 cut(s) 379
FaeI CATG 1 cut(s) 933
FaiI YATR 8 cut(s) 441, 551, 590, 607, 662, 711, 931, 984
FalI AAGNNNNNCTT 4 cut(s) 209, 241, 620, 652
FaqI GGGAC 1 cut(s) 591
FatI CATG 1 cut(s) 929
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 3 cut(s) 29, 252, 958
FokI GGATG 1 cut(s) 779
FriOI GRGCYC 1 cut(s) 438
Fsp4HI GCNGC 3 cut(s) 29, 252, 958
FspBI CTAG 1 cut(s) 509
GluI GCNGC 3 cut(s) 29, 252, 958
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 1 cut(s) 403
HaeIII GGCC 4 cut(s) 14, 53, 291, 735
HapII CCGG 2 cut(s) 288, 520
HgaI GACGC 1 cut(s) 257
Hin1I GRCGYC 2 cut(s) 249, 372
Hin1II CATG 1 cut(s) 933
HincII GTYRAC 2 cut(s) 654, 751
HindII GTYRAC 2 cut(s) 654, 751
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 5 cut(s) 34, 139, 538, 583, 854
HpaI GTTAAC 2 cut(s) 654, 751
HpaII CCGG 2 cut(s) 288, 520
HphI GGTGA 4 cut(s) 85, 151, 205, 541
Hpy166II GTNNAC 2 cut(s) 654, 751
Hpy188I TCNGA 1 cut(s) 271
Hpy188III TCNNGA 6 cut(s) 38, 352, 503, 542, 891, 917
Hpy8I GTNNAC 2 cut(s) 654, 751
Hpy99I CGWCG 4 cut(s) 65, 248, 251, 374
HpyAV CCTTC 5 cut(s) 81, 235, 725, 857, 983
HpyCH4III ACNGT 3 cut(s) 82, 415, 699
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 5 cut(s) 400, 535, 621, 640, 960
HpyF10VI GCNNNNNNNGC 1 cut(s) 642
HpyF3I CTNAG 4 cut(s) 147, 308, 462, 873
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 2 cut(s) 249, 372
Hsp92II CATG 1 cut(s) 933
Ksp22I TGATCA 1 cut(s) 478
KspAI GTTAAC 2 cut(s) 654, 751
Kzo9I GATC 5 cut(s) 43, 67, 88, 264, 478
LguI GCTCTTC 1 cut(s) 558
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 1 cut(s) 944
LweI GCATC 2 cut(s) 354, 522
MaeI CTAG 1 cut(s) 509
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 2 cut(s) 129, 457
MalI GATC 5 cut(s) 45, 69, 90, 266, 480
MboI GATC 5 cut(s) 43, 67, 88, 264, 478
MboII GAAGA 4 cut(s) 340, 575, 640, 836
MfeI CAATTG 1 cut(s) 858
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 3 cut(s) 498, 601, 858
MlyI GAGTC 3 cut(s) 148, 547, 863
MmeI TCCRAC 6 cut(s) 71, 127, 231, 283, 294, 744
Mph1103I ATGCAT 1 cut(s) 623
MseI TTAA 6 cut(s) 524, 600, 624, 653, 750, 837
MspA1I CMGCKG 1 cut(s) 957
MspI CCGG 2 cut(s) 288, 520
MspR9I CCNGG 1 cut(s) 11
MunI CAATTG 1 cut(s) 858
MvaI CCWGG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 642
NdeII GATC 5 cut(s) 43, 67, 88, 264, 478
NlaIII CATG 1 cut(s) 933
NlaIV GGNNCC 1 cut(s) 199
NmeAIII GCCGAG 2 cut(s) 79, 162
NsiI ATGCAT 1 cut(s) 623
NspV TTCGAA 1 cut(s) 515
PaeR7I CTCGAG 1 cut(s) 293
PceI AGGCCT 1 cut(s) 735
PciSI GCTCTTC 1 cut(s) 558
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 2 cut(s) 34, 583
PinAI ACCGGT 1 cut(s) 519
PkrI GCNGC 3 cut(s) 30, 253, 959
PleI GAGTC 3 cut(s) 147, 546, 862
PpsI GAGTC 3 cut(s) 147, 546, 862
Psp124BI GAGCTC 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 9
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 199
PspPI GGNCC 2 cut(s) 13, 276
PspXI VCTCGAGB 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 541
RsaI GTAC 1 cut(s) 701
RsaNI GTAC 1 cut(s) 700
SacI GAGCTC 1 cut(s) 438
SapI GCTCTTC 1 cut(s) 558
SaqAI TTAA 6 cut(s) 524, 600, 624, 653, 750, 837
SatI GCNGC 3 cut(s) 29, 252, 958
Sau3AI GATC 5 cut(s) 43, 67, 88, 264, 478
Sau96I GGNCC 2 cut(s) 13, 276
SchI GAGTC 3 cut(s) 148, 547, 863
ScrFI CCNGG 1 cut(s) 11
SduI GDGCHC 1 cut(s) 438
SfaNI GCATC 2 cut(s) 354, 522
Sfr274I CTCGAG 1 cut(s) 293
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 1 cut(s) 276
SlaI CTCGAG 1 cut(s) 293
SmlI CTYRAG 5 cut(s) 293, 428, 503, 689, 703
SmoI CTYRAG 5 cut(s) 293, 428, 503, 689, 703
Sse9I AATT 3 cut(s) 498, 601, 858
SseBI AGGCCT 1 cut(s) 735
SsiI CCGC 9 cut(s) 26, 29, 134, 162, 169, 229, 252, 279, 955
SspMI CTAG 1 cut(s) 509
SstI GAGCTC 1 cut(s) 438
StuI AGGCCT 1 cut(s) 735
StyD4I CCNGG 1 cut(s) 9
StyI CCWWGG 2 cut(s) 180, 744
TaaI ACNGT 3 cut(s) 82, 415, 699
TaiI ACGT 1 cut(s) 375
TaqI TCGA 9 cut(s) 87, 126, 243, 294, 369, 515, 804, 890, 918
TaqII GACCGA 1 cut(s) 112
TasI AATT 3 cut(s) 498, 601, 858
TatI WGTACW 1 cut(s) 699
TauI GCSGC 2 cut(s) 31, 254
TfiI GAWTC 2 cut(s) 34, 583
Tru1I TTAA 6 cut(s) 524, 600, 624, 653, 750, 837
Tru9I TTAA 6 cut(s) 524, 600, 624, 653, 750, 837
TscAI CASTG 2 cut(s) 151, 798
TseI GCWGC 1 cut(s) 957
TspDTI ATGAA 1 cut(s) 468
TspGWI ACGGA 1 cut(s) 595
TspRI CASTG 2 cut(s) 151, 798
VpaK11BI GGWCC 1 cut(s) 276
XapI RAATTY 1 cut(s) 498
XhoI CTCGAG 1 cut(s) 293
XspI CTAG 1 cut(s) 509
ZraI GACGTC 1 cut(s) 373
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.