Rw2G005660

Protein phosphatase 1 regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011350
Physical Location & Seq
Forward (+)
5387570 .. 5390144
2575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005660.1

Sequence Viewer

Length: 1011 bp
ATGGACGACCAGGCCCAACCACCACCGCCGAATCACGATGATCCGACGGCCGAGAACGACGGCGATCCTTCCTCCACCGTCCTCGATCTCACCAGCTTCCAACTCCACGACCTCCACTCGGTCGAGTTACCGCCGAGTCTCACCGAGTTAGACCTCACCGCCAACCGCCTCTCCTCCGTGGACACTCGCATTGGAACCCTCTCCAACCTCACCAAGCTCTCCTTCCGCCAGAACCTCATCGACGACGCCGCCGTCCAACCGATCTCCGCCTGGACCGCCCTCTCCGGCCTCGAGGAGTTGGTGCTGAGGGATAATAAGCTGAACAAAATACCTGATGTGAGCATCTTCAAGAAGCTTCTGGTCTTCGACGTCTCTTTCAATGAGATTACATCGTTGCAGGGGCTTTCCAAGGTCTCCAGCACCCTCAAGGAGCTCTATGTCTCCAAAAATGAAGTTACTAAGATTGAGGAGATTGATCATCTGCATCAGCTTCAAATTCTTGAGCTAGGTTCAAACCGGTTAAGGGTGATGCAGAGTCTCGAAAATATGACAAAGTTAGAAGAGCTATGGCTGGGACGGAATCGTATAAAGGTAGTTAATTTATGTGGGCTGAAATGCATTAAGAAGATTAGCTTGCAAAGCAATCGGTTAACTTCTATGACTGGATTTGAGGAATGTGTTGCGCTTGAGGAACTGTACTTGAGCCATAATGGGATTTCCAAAATGGAAGGCCTCTCAACCTTGGTTAACCTGCGTGTGTTGGATGTATCTAATAACAAGCTAACATCAGTGAATGATATCGAACACCTAACAATGTTAGAAGACCTGTGGCTTAATGACAACCAGATAGAGTCAATTGAAGGCGTTGCTGAGGCTGTTGTTGGTTCGAGAGAGAAGCTGGCTACTATCTATCTCGAACATAATCCATGTGCAAAAAATCCAAACTATTCCGCTGCATTGAAAAAAACCTTCCCGAACATACAGCAGATTGATTCTAATATATTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_6 LRR_8

Protein Analysis

336

Amino Acids

37.54

Weight (kDa)

4.95

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 229 - 268 1.8e-09 Leucine Rich repeats (2 copies)
LRR_8 PF13855 229 - 283 1.8e-06 Leucine rich repeat
LRR_9 PF14580 233 - 331 4.8e-06 Leucine-rich repeat
LRR_4 PF12799 250 - 289 6.5e-08 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19680
fragaria_vesca FvH4_1g05880 FvH4_1g05880
malus_domestica MD02G1060900.v1.1 MD15G1194400.v1.1
prunus_persica Prupe.7G222100_v2.0.a1 Prupe.7G222100_v2.0.a1
pyrus_communis pycom02g05000 pycom15g17400
rosa_chinensis RchiOBHm_Chr2g0091541 RchiOBHm_Chr2g0091621
rosa_laevigata RLG00000016234
rosa_multiflora Rmu_sc0002407.1_g000003
rosa_roxburghii Rroxscaffold_2G00149950
rosa_rugosa Rorug02G0018600
rosa_samantha Rh2AG063800 Rh2BG063200 Rh2CG064900 Rh2DG063300
rosa_wichuraiana Rw2G005660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AatII GACGTC 1 cut(s) 372
AbsI CCTCGAGG 1 cut(s) 290
Acc36I ACCTGC 1 cut(s) 759
AciI CCGC 9 cut(s) 26, 131, 159, 166, 226, 249, 267, 276, 951
AclWI GGATC 2 cut(s) 35, 59
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 495
AcyI GRCGYC 2 cut(s) 246, 369
AfaI GTAC 1 cut(s) 698
AfiI CCNNNNNNNGG 2 cut(s) 118, 523
AgeI ACCGGT 1 cut(s) 516
AgsI TTSAA 6 cut(s) 349, 379, 494, 513, 860, 961
AjnI CCWGG 2 cut(s) 9, 269
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
Alw21I GWGCWC 1 cut(s) 435
Alw26I GTCTC 5 cut(s) 143, 376, 418, 445, 542
AlwI GGATC 2 cut(s) 35, 59
Ama87I CYCGRG 1 cut(s) 290
AoxI GGCC 4 cut(s) 12, 48, 286, 730
ApeKI GCWGC 1 cut(s) 953
ApoI RAATTY 1 cut(s) 495
AsiGI ACCGGT 1 cut(s) 516
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 2 cut(s) 13, 273
AsuHPI GGTGA 5 cut(s) 82, 133, 148, 202, 538
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 1 cut(s) 273
BanII GRGCYC 1 cut(s) 435
BbsI GAAGAC 2 cut(s) 355, 828
Bbv12I GWGCWC 1 cut(s) 435
BbvCI CCTCAGC 2 cut(s) 305, 870
BbvI GCAGC 1 cut(s) 940
BceAI ACGGC 3 cut(s) 63, 76, 236
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 2 cut(s) 11, 271
BclI TGATCA 1 cut(s) 475
BcoDI GTCTC 5 cut(s) 143, 376, 418, 445, 542
BfaI CTAG 1 cut(s) 506
BfuAI ACCTGC 1 cut(s) 759
BisI GCNGC 2 cut(s) 249, 954
BlsI GCNGC 2 cut(s) 250, 955
Bme1390I CCNGG 2 cut(s) 11, 271
Bme18I GGWCC 1 cut(s) 273
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 2 cut(s) 13, 273
BmiI GGNNCC 1 cut(s) 196
BmrFI CCNGG 2 cut(s) 11, 271
BmsI GCATC 3 cut(s) 351, 493, 519
BpiI GAAGAC 2 cut(s) 355, 828
BpmI CTGGAG 1 cut(s) 400
Bpu10I CCTNAGC 2 cut(s) 305, 870
BpuEI CTTGAG 4 cut(s) 410, 521, 707, 721
BsaHI GRCGYC 2 cut(s) 246, 369
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 3 cut(s) 177, 408, 741
BsaWI WCCGGW 1 cut(s) 516
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 523
Bse118I RCCGGY 1 cut(s) 516
Bse1I ACTGG 1 cut(s) 667
BseBI CCWGG 2 cut(s) 11, 271
BseDI CCNNGG 3 cut(s) 177, 408, 741
BseGI GGATG 1 cut(s) 769
BseLI CCNNNNNNNGG 2 cut(s) 118, 523
BseMII CTCAG 2 cut(s) 296, 861
BseNI ACTGG 1 cut(s) 667
BseRI GAGGAG 3 cut(s) 163, 308, 482
BseX3I CGGCCG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 940
BseYI CCCAGC 1 cut(s) 571
Bsh1285I CGRYCG 2 cut(s) 51, 123
BshFI GGCC 4 cut(s) 14, 50, 288, 732
BshTI ACCGGT 1 cut(s) 516
BsiEI CGRYCG 2 cut(s) 51, 123
BsiHKAI GWGCWC 1 cut(s) 435
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 2 cut(s) 285, 517
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 2 cut(s) 118, 523
BsmAI GTCTC 5 cut(s) 143, 376, 418, 445, 542
BsmBI CGTCTC 1 cut(s) 376
BsmFI GGGAC 1 cut(s) 588
BsnI GGCC 4 cut(s) 14, 50, 288, 732
Bso31I GGTCTC 1 cut(s) 418
BsoBI CYCGRG 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 435
Bsp143I GATC 5 cut(s) 40, 64, 85, 261, 475
BspACI CCGC 9 cut(s) 26, 131, 159, 166, 226, 249, 267, 276, 951
BspANI GGCC 4 cut(s) 14, 50, 288, 732
BspCNI CTCAG 2 cut(s) 297, 862
BspLI GGNNCC 1 cut(s) 196
BspMI ACCTGC 1 cut(s) 759
BspPI GGATC 2 cut(s) 35, 59
BspQI GCTCTTC 1 cut(s) 555
BspTNI GGTCTC 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 516
BsrI ACTGG 1 cut(s) 667
BssAI RCCGGY 1 cut(s) 516
BssECI CCNNGG 3 cut(s) 177, 408, 741
BssMI GATC 5 cut(s) 40, 64, 85, 261, 475
BssNI GRCGYC 2 cut(s) 246, 369
BssT1I CCWWGG 2 cut(s) 408, 741
Bst2UI CCWGG 2 cut(s) 11, 271
Bst4CI ACNGT 2 cut(s) 79, 696
Bst6I CTCTTC 1 cut(s) 555
BstACI GRCGYC 2 cut(s) 246, 369
BstC8I GCNNGC 2 cut(s) 635, 900
BstDEI CTNAG 3 cut(s) 305, 459, 870
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 769
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 5 cut(s) 43, 67, 88, 264, 478
BstMAI GTCTC 5 cut(s) 143, 376, 418, 445, 542
BstMBI GATC 5 cut(s) 40, 64, 85, 261, 475
BstMCI CGRYCG 2 cut(s) 51, 123
BstMWI GCNNNNNNNGC 2 cut(s) 275, 639
BstNI CCWGG 2 cut(s) 11, 271
BstSCI CCNGG 2 cut(s) 9, 269
BstV1I GCAGC 1 cut(s) 940
BstV2I GAAGAC 2 cut(s) 355, 828
BstZI CGGCCG 1 cut(s) 48
BsuRI GGCC 4 cut(s) 14, 50, 288, 732
BtgI CCRYGG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 769
BtsIMutI CAGTG 1 cut(s) 795
BveI ACCTGC 1 cut(s) 759
Cac8I GCNNGC 2 cut(s) 635, 900
CfoI GCGC 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 516
Cfr13I GGNCC 2 cut(s) 13, 273
CseI GACGC 1 cut(s) 254
Csp6I GTAC 1 cut(s) 697
CspAI ACCGGT 1 cut(s) 516
CviAII CATG 1 cut(s) 927
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 3 cut(s) 305, 459, 870
DpnI GATC 5 cut(s) 42, 66, 87, 263, 477
DpnII GATC 5 cut(s) 40, 64, 85, 261, 475
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 1 cut(s) 48
EagI CGGCCG 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
EciI GGCGGA 2 cut(s) 215, 256
Ecl136II GAGCTC 1 cut(s) 433
EclXI CGGCCG 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 408, 741
Eco147I AGGCCT 1 cut(s) 732
Eco24I GRGCYC 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 418
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 1 cut(s) 273
Eco52I CGGCCG 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 433
Eco88I CYCGRG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 433
EcoRII CCWGG 2 cut(s) 9, 269
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 2 cut(s) 408, 741
EcoT22I ATGCAT 1 cut(s) 620
EcoT38I GRGCYC 1 cut(s) 435
ErhI CCWWGG 2 cut(s) 408, 741
Esp3I CGTCTC 1 cut(s) 376
FaeI CATG 1 cut(s) 930
FalI AAGNNNNNCTT 4 cut(s) 206, 238, 617, 649
FaqI GGGAC 1 cut(s) 588
FatI CATG 1 cut(s) 926
FbaI TGATCA 1 cut(s) 475
Fnu4HI GCNGC 2 cut(s) 249, 954
FokI GGATG 1 cut(s) 776
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 2 cut(s) 249, 954
FspBI CTAG 1 cut(s) 506
GlaI GCGC 1 cut(s) 684
GluI GCNGC 2 cut(s) 249, 954
GsaI CCCAGC 1 cut(s) 575
GsuI CTGGAG 1 cut(s) 400
HaeIII GGCC 4 cut(s) 14, 50, 288, 732
HapII CCGG 2 cut(s) 285, 517
HgaI GACGC 1 cut(s) 254
HhaI GCGC 1 cut(s) 685
Hin1I GRCGYC 2 cut(s) 246, 369
Hin1II CATG 1 cut(s) 930
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HincII GTYRAC 2 cut(s) 651, 748
HindII GTYRAC 2 cut(s) 651, 748
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 6 cut(s) 31, 136, 535, 580, 851, 992
HpaI GTTAAC 2 cut(s) 651, 748
HpaII CCGG 2 cut(s) 285, 517
HphI GGTGA 5 cut(s) 82, 133, 148, 202, 538
Hpy166II GTNNAC 3 cut(s) 181, 651, 748
Hpy188I TCNGA 1 cut(s) 45
Hpy188III TCNNGA 7 cut(s) 35, 349, 500, 539, 888, 914, 973
Hpy8I GTNNAC 3 cut(s) 181, 651, 748
Hpy99I CGWCG 5 cut(s) 49, 62, 245, 248, 371
HpyAV CCTTC 5 cut(s) 78, 232, 722, 854, 979
HpyCH4III ACNGT 2 cut(s) 79, 696
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 7 cut(s) 397, 484, 532, 618, 637, 932, 956
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 639
HpyF3I CTNAG 3 cut(s) 305, 459, 870
HpySE526I ACGT 1 cut(s) 369
Hsp92I GRCGYC 2 cut(s) 246, 369
Hsp92II CATG 1 cut(s) 930
HspAI GCGC 1 cut(s) 683
Ksp22I TGATCA 1 cut(s) 475
KspAI GTTAAC 2 cut(s) 651, 748
Kzo9I GATC 5 cut(s) 40, 64, 85, 261, 475
LguI GCTCTTC 1 cut(s) 555
LmnI GCTCC 1 cut(s) 430
Lsp1109I GCAGC 1 cut(s) 940
LweI GCATC 3 cut(s) 351, 493, 519
MaeI CTAG 1 cut(s) 506
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 2 cut(s) 126, 454
MalI GATC 5 cut(s) 42, 66, 87, 263, 477
MboI GATC 5 cut(s) 40, 64, 85, 261, 475
MboII GAAGA 5 cut(s) 337, 355, 572, 637, 833
MfeI CAATTG 1 cut(s) 855
MhlI GDGCHC 1 cut(s) 435
MluCI AATT 3 cut(s) 495, 598, 855
MlyI GAGTC 3 cut(s) 145, 544, 860
MmeI TCCRAC 5 cut(s) 68, 124, 228, 280, 741
Mph1103I ATGCAT 1 cut(s) 620
MseI TTAA 7 cut(s) 521, 597, 621, 650, 747, 834, 1009
MspA1I CMGCKG 1 cut(s) 953
MspI CCGG 2 cut(s) 285, 517
MspR9I CCNGG 2 cut(s) 11, 271
MunI CAATTG 1 cut(s) 855
MvaI CCWGG 2 cut(s) 11, 271
MwoI GCNNNNNNNGC 2 cut(s) 275, 639
NdeII GATC 5 cut(s) 40, 64, 85, 261, 475
NlaIII CATG 1 cut(s) 930
NlaIV GGNNCC 1 cut(s) 196
NmeAIII GCCGAG 2 cut(s) 76, 159
NsiI ATGCAT 1 cut(s) 620
PaeR7I CTCGAG 1 cut(s) 290
PceI AGGCCT 1 cut(s) 732
PciSI GCTCTTC 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 3 cut(s) 31, 580, 992
PinAI ACCGGT 1 cut(s) 516
PkrI GCNGC 2 cut(s) 250, 955
PleI GAGTC 3 cut(s) 144, 543, 859
PpsI GAGTC 3 cut(s) 144, 543, 859
Psp124BI GAGCTC 1 cut(s) 435
Psp6I CCWGG 2 cut(s) 9, 269
PspFI CCCAGC 1 cut(s) 571
PspGI CCWGG 2 cut(s) 9, 269
PspN4I GGNNCC 1 cut(s) 196
PspPI GGNCC 2 cut(s) 13, 273
PspXI VCTCGAGB 1 cut(s) 290
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
SacI GAGCTC 1 cut(s) 435
SapI GCTCTTC 1 cut(s) 555
SaqAI TTAA 7 cut(s) 521, 597, 621, 650, 747, 834, 1009
SatI GCNGC 2 cut(s) 249, 954
Sau3AI GATC 5 cut(s) 40, 64, 85, 261, 475
Sau96I GGNCC 2 cut(s) 13, 273
SchI GAGTC 3 cut(s) 145, 544, 860
ScrFI CCNGG 2 cut(s) 11, 271
SduI GDGCHC 1 cut(s) 435
SfaNI GCATC 3 cut(s) 351, 493, 519
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 1 cut(s) 273
SlaI CTCGAG 1 cut(s) 290
SmlI CTYRAG 5 cut(s) 290, 425, 500, 686, 700
SmoI CTYRAG 5 cut(s) 290, 425, 500, 686, 700
Sse9I AATT 3 cut(s) 495, 598, 855
SseBI AGGCCT 1 cut(s) 732
SsiI CCGC 9 cut(s) 26, 131, 159, 166, 226, 249, 267, 276, 951
SspMI CTAG 1 cut(s) 506
SstI GAGCTC 1 cut(s) 435
StuI AGGCCT 1 cut(s) 732
StyD4I CCNGG 2 cut(s) 9, 269
StyI CCWWGG 2 cut(s) 408, 741
TaaI ACNGT 2 cut(s) 79, 696
TaiI ACGT 1 cut(s) 372
TaqI TCGA 9 cut(s) 84, 123, 240, 291, 366, 540, 801, 887, 915
TaqII GACCGA 1 cut(s) 109
TasI AATT 3 cut(s) 495, 598, 855
TatI WGTACW 1 cut(s) 696
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 3 cut(s) 31, 580, 992
Tru1I TTAA 7 cut(s) 521, 597, 621, 650, 747, 834, 1009
Tru9I TTAA 7 cut(s) 521, 597, 621, 650, 747, 834, 1009
TscAI CASTG 1 cut(s) 795
TseI GCWGC 1 cut(s) 953
TspDTI ATGAA 1 cut(s) 465
TspGWI ACGGA 2 cut(s) 166, 592
TspRI CASTG 1 cut(s) 795
VpaK11BI GGWCC 1 cut(s) 273
XapI RAATTY 1 cut(s) 495
XhoI CTCGAG 1 cut(s) 290
XspI CTAG 1 cut(s) 506
ZraI GACGTC 1 cut(s) 370
Zsp2I ATGCAT 1 cut(s) 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.