MD02G1082800.v1.1

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012493
Physical Location & Seq
Reverse (-)
6474700 .. 6479584
4885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1082800.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGTGCGGCGATGCAACGAACTGGGACGAAGAAGCATACAGAGAATCCATATTAAAGGAGCGAGAGATCCAAACCCGAACAGTTTTCCGAACCGTTTGGGCTCCGTCGCTTAACCCTAACCCGGACACCGTCGTCGTCGCCTCCAGCGACGGATCGGTTTCCTCCTACTCCATCCCCTCCCTTATCTCCAAGCTCCCACTGGGTTTCAGCAATGTGAAATCTCCAAATGTTTGTAGGTCGTTAATGGCGGAACCTACTTGCTTCCTTCAAGCTCATGAAGGACCTGCTTATGATGTTAAGTTTTACGGAAATGGCGAAGATTCTGTATTGTTGAGTTGCGGTGATGATGGACGGATTCAAGGATGGAGGTGGAAGGATTGGGAGGAATCGGATGTGCCTATTCATTTGCAAGGAAAGCATGTGAAGCCCATAATTGACCTTGTGAACCCCCAACATAAAGGTCCATGGGGGGCTCTATCTCCAATCCCAGAAAACAATGCTCTTGCTGTTTACAACGAGGGGGGATGCATTTTTTCTGCTGCTGGTGATTCTTGCGTCTATTGCTGGGATGTGGAAAGAGGTCAAGTCAAAATGACTTTTAAGGGGCATTCAGATTACTTGCATTCTGTAGTTGCTCGGAACTCTACCAATCAGATCATAACTGGTTCAGAGGATGGGACAGCACGGATATGGGATTGCAAAAGTGGAAAGTGCGTTCAAGTATATGAGCCAGCCAAGGGTATTAAGTTGAAAGGATTTTTTTCATGCGTCAGTTGCATTGCTCTTGATGCAAGTGAAAGCTGGTTGGCTTGCGGTAGTGGTCAGAGTTTATCAGTTTGGAACCTTCCTGCTTCTGAATGCATTTCAAGGGTTTCAACTTGTGCATCCGTACAGGATGTAGTATTCGATGAAAATCAAGTATTAGCAGTTGGAGCAGAACCTCTACTTTCCCGTTTTGACATAAATGGGGCGATTCTTTCACAAATGCAATGTGCTCCTCAGTCTGCCTTTTCCGTCTCATTACACCCATCTGGGGTTACCGCAGTAGGAGGTTACGGTGGTCTTGTGGATGTTATTTCGCAGTTCGGAAGCCACTTGTGCACATTCGGCTGCCGATGCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C WD40

Protein Analysis

375

Amino Acids

40.39

Weight (kDa)

5.12

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 87 - 241 5e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 143 - 282 1.3e-11 WDR41
WD40_Prp19 PF24814 166 - 293 7.9e-13 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 166 - 241 7.8e-08 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 167 - 242 5.2e-12 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 167 - 243 2.5e-11 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 168 - 241 1.2e-13 WDR5 beta-propeller domain
WDR55 PF24796 171 - 288 3.7e-11 WDR55
EIF3I PF24805 172 - 250 2.6e-11 EIF3I
WD40_CDC20-Fz PF24807 175 - 306 4.9e-09 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 175 - 234 1.7e-06 TEP-1 second beta-propeller
WD40_Gbeta PF25391 176 - 243 6.1e-09 G protein beta WD-40 repeat protein
WD40 PF00400 195 - 232 2.2e-06 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
Acc36I ACCTGC 1 cut(s) 292
AciI CCGC 5 cut(s) 6, 248, 339, 813, 1041
AclWI GGATC 2 cut(s) 61, 160
AfaI GTAC 1 cut(s) 891
AfiI CCNNNNNNNGG 3 cut(s) 121, 737, 1033
AgsI TTSAA 6 cut(s) 269, 359, 719, 751, 867, 876
AloI GAACNNNNNNTCC 2 cut(s) 699, 731
AluBI AGCT 3 cut(s) 193, 272, 801
AluI AGCT 3 cut(s) 193, 272, 801
Alw21I GWGCWC 2 cut(s) 997, 1103
Alw26I GTCTC 1 cut(s) 1021
Alw44I GTGCAC 1 cut(s) 1099
AlwI GGATC 2 cut(s) 61, 160
ApaLI GTGCAC 1 cut(s) 1099
ApeKI GCWGC 2 cut(s) 539, 1110
AspS9I GGNCC 2 cut(s) 281, 461
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 353, 557
AvaII GGWCC 2 cut(s) 281, 461
BaeGI GKGCMC 1 cut(s) 1103
BanII GRGCYC 2 cut(s) 103, 475
Bbv12I GWGCWC 2 cut(s) 997, 1103
BbvI GCAGC 2 cut(s) 526, 1097
BccI CCATC 5 cut(s) 179, 341, 357, 668, 1036
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 1021
BfmI CTRYAG 1 cut(s) 627
BfuAI ACCTGC 1 cut(s) 292
BisI GCNGC 3 cut(s) 7, 540, 1111
BlsI GCNGC 3 cut(s) 8, 541, 1112
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 2 cut(s) 281, 461
BmgT120I GGNCC 2 cut(s) 281, 461
BmiI GGNNCC 3 cut(s) 102, 252, 842
BmrFI CCNGG 1 cut(s) 122
BmrI ACTGGG 2 cut(s) 31, 209
BmsI GCATC 4 cut(s) 515, 778, 893, 1106
BmuI ACTGGG 2 cut(s) 31, 209
BpmI CTGGAG 1 cut(s) 127
BpuMI CCSGG 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 912
BsaJI CCNNGG 2 cut(s) 464, 735
Bsc4I CCNNNNNNNGG 3 cut(s) 121, 737, 1033
Bse1I ACTGG 3 cut(s) 26, 204, 667
Bse3DI GCAATG 3 cut(s) 217, 777, 995
Bse8I GATNNNNATC 1 cut(s) 912
BseDI CCNNGG 2 cut(s) 464, 735
BseGI GGATG 9 cut(s) 171, 368, 397, 530, 574, 679, 884, 901, 1075
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 3 cut(s) 121, 737, 1033
BseMI GCAATG 3 cut(s) 217, 777, 995
BseMII CTCAG 1 cut(s) 1013
BseNI ACTGG 3 cut(s) 26, 204, 667
BseRI GAGGAG 1 cut(s) 987
BseSI GKGCMC 1 cut(s) 1103
BseXI GCAGC 2 cut(s) 526, 1097
BseYI CCCAGC 1 cut(s) 564
BsiHKAI GWGCWC 2 cut(s) 997, 1103
BsiSI CCGG 1 cut(s) 122
BslFI GGGAC 2 cut(s) 38, 691
BslI CCNNNNNNNGG 3 cut(s) 121, 737, 1033
BsmAI GTCTC 1 cut(s) 1021
BsmBI CGTCTC 1 cut(s) 1021
BsmFI GGGAC 2 cut(s) 38, 691
BsmI GAATGC 3 cut(s) 607, 622, 863
Bsp1286I GDGCHC 4 cut(s) 103, 475, 997, 1103
Bsp143I GATC 3 cut(s) 66, 152, 654
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 5 cut(s) 6, 248, 339, 813, 1041
BspCNI CTCAG 1 cut(s) 1012
BspHI TCATGA 1 cut(s) 274
BspLI GGNNCC 3 cut(s) 102, 252, 842
BspMI ACCTGC 1 cut(s) 292
BspPI GGATC 2 cut(s) 61, 160
BsrDI GCAATG 3 cut(s) 217, 777, 995
BsrI ACTGG 3 cut(s) 26, 204, 667
BssECI CCNNGG 2 cut(s) 464, 735
BssMI GATC 3 cut(s) 66, 152, 654
BssT1I CCWWGG 2 cut(s) 464, 735
Bst4CI ACNGT 4 cut(s) 82, 94, 130, 1058
BstC8I GCNNGC 2 cut(s) 732, 811
BstDEI CTNAG 1 cut(s) 999
BstDSI CCRYGG 1 cut(s) 464
BstEII GGTNACC 1 cut(s) 1036
BstF5I GGATG 9 cut(s) 171, 368, 397, 530, 574, 679, 884, 901, 1075
BstKTI GATC 3 cut(s) 69, 155, 657
BstMAI GTCTC 1 cut(s) 1021
BstMBI GATC 3 cut(s) 66, 152, 654
BstMWI GCNNNNNNNGC 9 cut(s) 415, 424, 561, 774, 788, 932, 1098, 1107, 1116
BstNSI RCATGY 1 cut(s) 422
BstPI GGTNACC 1 cut(s) 1036
BstSCI CCNGG 1 cut(s) 120
BstSFI CTRYAG 1 cut(s) 627
BstSLI GKGCMC 1 cut(s) 1103
BstV1I GCAGC 2 cut(s) 526, 1097
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 464
BtgZI GCGATG 1 cut(s) 24
BtsCI GGATG 9 cut(s) 171, 368, 397, 530, 574, 679, 884, 901, 1075
BtsIMutI CAGTG 1 cut(s) 197
BveI ACCTGC 1 cut(s) 292
Cac8I GCNNGC 2 cut(s) 732, 811
CciI TCATGA 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 281, 461
CseI GACGC 2 cut(s) 544, 757
Csp6I GTAC 1 cut(s) 890
CviAII CATG 4 cut(s) 275, 419, 465, 765
CviQI GTAC 1 cut(s) 890
DdeI CTNAG 1 cut(s) 999
DpnI GATC 3 cut(s) 68, 154, 656
DpnII GATC 3 cut(s) 66, 152, 654
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 263
Eco130I CCWWGG 2 cut(s) 464, 735
Eco24I GRGCYC 2 cut(s) 103, 475
Eco47I GGWCC 2 cut(s) 281, 461
Eco91I GGTNACC 1 cut(s) 1036
EcoO109I RGGNCCY 1 cut(s) 281
EcoO65I GGTNACC 1 cut(s) 1036
EcoT14I CCWWGG 2 cut(s) 464, 735
EcoT22I ATGCAT 2 cut(s) 530, 863
EcoT38I GRGCYC 2 cut(s) 103, 475
ErhI CCWWGG 2 cut(s) 464, 735
Esp3I CGTCTC 1 cut(s) 1021
FaeI CATG 4 cut(s) 278, 422, 468, 768
FaqI GGGAC 2 cut(s) 38, 691
FatI CATG 4 cut(s) 274, 418, 464, 764
Fnu4HI GCNGC 3 cut(s) 7, 540, 1111
FokI GGATG 9 cut(s) 158, 375, 404, 537, 581, 686, 871, 908, 1082
FriOI GRGCYC 2 cut(s) 103, 475
Fsp4HI GCNGC 3 cut(s) 7, 540, 1111
GluI GCNGC 3 cut(s) 7, 540, 1111
GsaI CCCAGC 1 cut(s) 568
GsuI CTGGAG 1 cut(s) 127
HapII CCGG 1 cut(s) 122
HgaI GACGC 2 cut(s) 544, 757
Hin1II CATG 4 cut(s) 278, 422, 468, 768
HinfI GANTC 6 cut(s) 44, 320, 355, 386, 548, 973
HpaII CCGG 1 cut(s) 122
HphI GGTGA 2 cut(s) 353, 557
Hpy166II GTNNAC 3 cut(s) 445, 511, 1101
Hpy188I TCNGA 9 cut(s) 89, 391, 613, 639, 654, 670, 825, 856, 1088
Hpy188III TCNNGA 2 cut(s) 275, 785
Hpy8I GTNNAC 3 cut(s) 445, 511, 1101
Hpy99I CGWCG 5 cut(s) 109, 134, 137, 140, 152
HpyAV CCTTC 4 cut(s) 272, 275, 367, 854
HpyCH4III ACNGT 4 cut(s) 82, 94, 130, 1058
HpyF10VI GCNNNNNNNGC 9 cut(s) 415, 424, 561, 774, 788, 932, 1098, 1107, 1116
HpyF3I CTNAG 1 cut(s) 999
Hsp92II CATG 4 cut(s) 278, 422, 468, 768
Kzo9I GATC 3 cut(s) 66, 152, 654
LmnI GCTCC 5 cut(s) 58, 106, 198, 932, 1000
Lsp1109I GCAGC 2 cut(s) 526, 1097
LweI GCATC 4 cut(s) 515, 778, 893, 1106
MaeIII GTNAC 2 cut(s) 1036, 1052
MalI GATC 3 cut(s) 68, 154, 656
MboI GATC 3 cut(s) 66, 152, 654
MboII GAAGA 2 cut(s) 41, 329
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 4 cut(s) 103, 475, 997, 1103
MluCI AATT 1 cut(s) 432
MmeI TCCRAC 1 cut(s) 910
Mph1103I ATGCAT 2 cut(s) 530, 863
MseI TTAA 6 cut(s) 53, 111, 242, 297, 600, 744
MslI CAYNNNNRTG 1 cut(s) 688
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 1 cut(s) 122
Mva1269I GAATGC 3 cut(s) 607, 622, 863
MwoI GCNNNNNNNGC 9 cut(s) 415, 424, 561, 774, 788, 932, 1098, 1107, 1116
NciI CCSGG 1 cut(s) 122
NcoI CCATGG 1 cut(s) 464
NdeII GATC 3 cut(s) 66, 152, 654
NlaIII CATG 4 cut(s) 278, 422, 468, 768
NlaIV GGNNCC 3 cut(s) 102, 252, 842
NsiI ATGCAT 2 cut(s) 530, 863
NspI RCATGY 1 cut(s) 422
PagI TCATGA 1 cut(s) 274
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 312
PctI GAATGC 3 cut(s) 607, 622, 863
PfeI GAWTC 6 cut(s) 44, 320, 355, 386, 548, 973
PflFI GACNNNGTC 1 cut(s) 128
PkrI GCNGC 3 cut(s) 8, 541, 1112
PpuMI RGGWCCY 1 cut(s) 281
Psp5II RGGWCCY 1 cut(s) 281
PspEI GGTNACC 1 cut(s) 1036
PspFI CCCAGC 1 cut(s) 564
PspN4I GGNNCC 3 cut(s) 102, 252, 842
PspPI GGNCC 2 cut(s) 281, 461
PspPPI RGGWCCY 1 cut(s) 281
PsuI RGATCY 1 cut(s) 66
PsyI GACNNNGTC 1 cut(s) 128
RsaI GTAC 1 cut(s) 891
RsaNI GTAC 1 cut(s) 890
RseI CAYNNNNRTG 1 cut(s) 688
SaqAI TTAA 6 cut(s) 53, 111, 242, 297, 600, 744
SatI GCNGC 3 cut(s) 7, 540, 1111
Sau3AI GATC 3 cut(s) 66, 152, 654
Sau96I GGNCC 2 cut(s) 281, 461
ScrFI CCNGG 1 cut(s) 122
SduI GDGCHC 4 cut(s) 103, 475, 997, 1103
SfaNI GCATC 4 cut(s) 515, 778, 893, 1106
SfcI CTRYAG 1 cut(s) 627
SinI GGWCC 2 cut(s) 281, 461
SmiMI CAYNNNNRTG 1 cut(s) 688
Sse9I AATT 1 cut(s) 432
SsiI CCGC 5 cut(s) 6, 248, 339, 813, 1041
StyD4I CCNGG 1 cut(s) 120
StyI CCWWGG 2 cut(s) 464, 735
TaaI ACNGT 4 cut(s) 82, 94, 130, 1058
TaqI TCGA 1 cut(s) 906
TasI AATT 1 cut(s) 432
TauI GCSGC 1 cut(s) 9
TfiI GAWTC 6 cut(s) 44, 320, 355, 386, 548, 973
Tru1I TTAA 6 cut(s) 53, 111, 242, 297, 600, 744
Tru9I TTAA 6 cut(s) 53, 111, 242, 297, 600, 744
TscAI CASTG 1 cut(s) 204
TseI GCWGC 2 cut(s) 539, 1110
TspDTI ATGAA 4 cut(s) 291, 392, 753, 924
TspGWI ACGGA 7 cut(s) 93, 165, 321, 367, 700, 877, 1003
TspRI CASTG 1 cut(s) 204
Tth111I GACNNNGTC 1 cut(s) 128
VneI GTGCAC 1 cut(s) 1099
VpaK11BI GGWCC 2 cut(s) 281, 461
XceI RCATGY 1 cut(s) 422
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
Zsp2I ATGCAT 2 cut(s) 530, 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.