Rroxscaffold_2G00147790

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012493
Physical Location & Seq
Forward (+)
85490973 .. 85493810
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00147790.1

Sequence Viewer

Length: 1110 bp
ATGTGCGGCGACGCAAGGAACTGGGACGAAGAAGCCTACAGGGAAACCATTCTAAAGGAGCGAGAGATCCAAACCCGAACCGTTTTCCGAACCGCCTGGGCTCCCTCCCTTAACCCTAACCCCGACACCATCGTCGTCGCCTCCAGCGATGGATCCGTTTCTTCCTACTCCATCCCTCACCTCGTCTCCAAGCTCCCACTAGGTTTCAGCAATGTGAAAGCTCCATACTTGTTAGTGGCGGAACCTAATTGCTTCCTTAAAGCGCATGAGGGGCCTGCATATGATGTTAAATTTTATGGCCACGACGAGGAGTCTATGCTGCTAAGCTGTGGGGACGATGGTCGAATTCGAGGATGGAGGTGGAAGGATTGTATACAGTCCGACGTGCCTATTCATTTGCAAGGAGACCAAGTGAAGCCAGTACTTGACCTGGTGAATCCTCAACATAAGGGGTCCTTGGGGGCTTTATCTCCAATTCCTGAAAACAATGCTCTTGCTGTTGATAATCAAGGAGTGTCCATTTTCTCTGCTGCTGGCGATTCTTGTGTCTATTGCTGGGATGTGGAAACAGGTCAAGTCAAAATTATTTTTAAGGGGCATTCAGATTACTTGCATTCCATAGTCGCCCGAAACTCTACAAATCAGATCATAACTGGTTCAGAGGATGGGACGGCACGTATATGGGATTGCAAAAGTGGAAAGTCAGTTCAAGTGATTGAGCCATTAAAGAATAATAAATTGAAAGGGTCGTGCGTCAGTTGCATTGCTCTTGATGCAAGTGAAAGCTGGTTGGCTTGTGGTACTGGTCGGAGTTTATCAGTTTGGAACCTTCCTGCTTCTGAATGCATTTCAAGGACTTCAACTCGTGCATCTGTACAGGATGTAGTATTTGATGAAAATCAAATATTAGCAGTTGGAGCAGAGCCTCTACTTAGCCGTTTTGACATAAATGGGGTGATTCTTTCACAAATGCAATGTGCCCCTCAGTCAGCCTTTTCTGTCTCATTACACGCATCTGGGGTCACAGCAATAGGAGGTTATGGAGGAGTTGTGGATGTTATTTCCCAGTTTGGAAGCCATTTGTGCACGTTTGGCTGCAAATGTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C WD40

Protein Analysis

369

Amino Acids

39.86

Weight (kDa)

5.38

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 88 - 244 5.2e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 163 - 305 1.7e-12 THOC3 beta-propeller domain
WD40_Prp19 PF24814 163 - 291 5.2e-12 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 163 - 243 8.8e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 164 - 276 6.3e-13 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 164 - 300 7.9e-14 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 164 - 268 7.9e-06 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 165 - 285 3.9e-11 WDR55
Beta-prop_WDR41 PF25178 167 - 287 2.6e-12 WDR41
EIF3I PF24805 168 - 240 2.5e-08 EIF3I
WD40_CDC20-Fz PF24807 168 - 300 3e-10 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 173 - 238 2.4e-08 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 174 - 231 9.6e-06 TEP-1 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AccI GTMKAC 1 cut(s) 373
AciI CCGC 3 cut(s) 6, 93, 239
AclWI GGATC 3 cut(s) 61, 147, 160
AcoI YGGCCR 1 cut(s) 298
AcsI RAATTY 2 cut(s) 290, 345
AfaI GTAC 3 cut(s) 423, 802, 876
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 4 cut(s) 710, 742, 852, 861
AhdI GACNNNNNGTC 1 cut(s) 310
AjiI CACGTC 1 cut(s) 385
AjnI CCWGG 2 cut(s) 95, 429
AloI GAACNNNNNNTCC 2 cut(s) 690, 722
AluBI AGCT 4 cut(s) 193, 221, 327, 786
AluI AGCT 4 cut(s) 193, 221, 327, 786
Alw21I GWGCWC 1 cut(s) 1088
Alw26I GTCTC 3 cut(s) 190, 399, 1006
Alw44I GTGCAC 1 cut(s) 1084
AlwI GGATC 3 cut(s) 61, 147, 160
AoxI GGCC 2 cut(s) 272, 298
ApaLI GTGCAC 1 cut(s) 1084
ApeKI GCWGC 3 cut(s) 319, 530, 1095
ApoI RAATTY 2 cut(s) 290, 345
Asp700I GAANNNNTTC 1 cut(s) 48
AspLEI GCGC 1 cut(s) 265
AspS9I GGNCC 2 cut(s) 272, 453
AsuHPI GGTGA 3 cut(s) 170, 445, 967
AvaII GGWCC 1 cut(s) 453
BaeGI GKGCMC 2 cut(s) 982, 1088
BalI TGGCCA 1 cut(s) 300
BamHI GGATCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 103
BauI CACGAG 1 cut(s) 864
Bbv12I GWGCWC 1 cut(s) 1088
BbvI GCAGC 3 cut(s) 306, 517, 1082
BccI CCATC 6 cut(s) 137, 143, 179, 332, 348, 659
BceAI ACGGC 2 cut(s) 687, 921
BciT130I CCWGG 2 cut(s) 97, 431
BcoDI GTCTC 3 cut(s) 190, 399, 1006
BfaI CTAG 1 cut(s) 200
BfmI CTRYAG 1 cut(s) 37
BisI GCNGC 4 cut(s) 7, 320, 531, 1096
BlpI GCTNAGC 1 cut(s) 323
BlsI GCNGC 4 cut(s) 8, 321, 532, 1097
BmcAI AGTACT 1 cut(s) 423
Bme1390I CCNGG 2 cut(s) 97, 431
Bme18I GGWCC 1 cut(s) 453
BmeRI GACNNNNNGTC 1 cut(s) 310
BmgBI CACGTC 1 cut(s) 385
BmgT120I GGNCC 2 cut(s) 272, 453
BmiI GGNNCC 6 cut(s) 102, 154, 243, 273, 454, 827
BmrFI CCNGG 2 cut(s) 97, 431
BmrI ACTGGG 2 cut(s) 31, 1060
BmsI GCATC 3 cut(s) 763, 878, 1022
BmuI ACTGGG 2 cut(s) 31, 1060
BoxI GACNNNNGTC 1 cut(s) 339
BpmI CTGGAG 1 cut(s) 127
Bpu1102I GCTNAGC 1 cut(s) 323
BsaAI YACGTR 1 cut(s) 677
BsaBI GATNNNNATC 1 cut(s) 897
BsaI GGTCTC 1 cut(s) 399
BsaJI CCNNGG 2 cut(s) 96, 456
BsaXI ACNNNNNCTCC 6 cut(s) 170, 200, 396, 426, 1035, 1065
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 5 cut(s) 26, 419, 658, 808, 1066
Bse3DI GCAATG 3 cut(s) 217, 762, 980
Bse8I GATNNNNATC 1 cut(s) 897
BseBI CCWGG 2 cut(s) 97, 431
BseDI CCNNGG 2 cut(s) 96, 456
BseGI GGATG 6 cut(s) 171, 359, 565, 670, 886, 1060
BseJI GATNNNNATC 1 cut(s) 897
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMI GCAATG 3 cut(s) 217, 762, 980
BseMII CTCAG 1 cut(s) 998
BseNI ACTGG 5 cut(s) 26, 419, 658, 808, 1066
BseRI GAGGAG 2 cut(s) 323, 1059
BseSI GKGCMC 2 cut(s) 982, 1088
BseXI GCAGC 3 cut(s) 306, 517, 1082
BseYI CCCAGC 1 cut(s) 555
BshFI GGCC 2 cut(s) 274, 300
BsiHKAI GWGCWC 1 cut(s) 1088
BslFI GGGAC 3 cut(s) 38, 347, 682
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 3 cut(s) 190, 399, 1006
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 3 cut(s) 38, 347, 682
BsmI GAATGC 3 cut(s) 598, 613, 848
BsnI GGCC 2 cut(s) 274, 300
Bso31I GGTCTC 1 cut(s) 399
Bsp1286I GDGCHC 3 cut(s) 103, 982, 1088
Bsp1407I TGTACA 1 cut(s) 874
Bsp143I GATC 3 cut(s) 66, 152, 645
Bsp1720I GCTNAGC 1 cut(s) 323
BspACI CCGC 3 cut(s) 6, 93, 239
BspANI GGCC 2 cut(s) 274, 300
BspCNI CTCAG 1 cut(s) 997
BspLI GGNNCC 6 cut(s) 102, 154, 243, 273, 454, 827
BspPI GGATC 3 cut(s) 61, 147, 160
BspTNI GGTCTC 1 cut(s) 399
BsrDI GCAATG 3 cut(s) 217, 762, 980
BsrGI TGTACA 1 cut(s) 874
BsrI ACTGG 5 cut(s) 26, 419, 658, 808, 1066
BssECI CCNNGG 2 cut(s) 96, 456
BssMI GATC 3 cut(s) 66, 152, 645
BssNAI GTATAC 1 cut(s) 374
BssSI CACGAG 1 cut(s) 864
BssT1I CCWWGG 1 cut(s) 456
Bst1107I GTATAC 1 cut(s) 374
Bst2BI CACGAG 1 cut(s) 864
Bst2UI CCWGG 2 cut(s) 97, 431
Bst4CI ACNGT 2 cut(s) 82, 378
BstAUI TGTACA 1 cut(s) 874
BstBAI YACGTR 1 cut(s) 677
BstC8I GCNNGC 2 cut(s) 276, 535
BstDEI CTNAG 3 cut(s) 323, 932, 984
BstF5I GGATG 6 cut(s) 171, 359, 565, 670, 886, 1060
BstHHI GCGC 1 cut(s) 265
BstKTI GATC 3 cut(s) 69, 155, 648
BstMAI GTCTC 3 cut(s) 190, 399, 1006
BstMBI GATC 3 cut(s) 66, 152, 645
BstMWI GCNNNNNNNGC 6 cut(s) 271, 759, 773, 917, 1083, 1092
BstNI CCWGG 2 cut(s) 97, 431
BstPAI GACNNNNGTC 1 cut(s) 339
BstSCI CCNGG 2 cut(s) 95, 429
BstSFI CTRYAG 1 cut(s) 37
BstSLI GKGCMC 2 cut(s) 982, 1088
BstV1I GCAGC 3 cut(s) 306, 517, 1082
BstX2I RGATCY 2 cut(s) 66, 152
BstYI RGATCY 2 cut(s) 66, 152
BstZ17I GTATAC 1 cut(s) 374
BsuRI GGCC 2 cut(s) 274, 300
BtgZI GCGATG 1 cut(s) 162
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 6 cut(s) 171, 359, 565, 670, 886, 1060
Cac8I GCNNGC 2 cut(s) 276, 535
CfoI GCGC 1 cut(s) 265
Cfr13I GGNCC 2 cut(s) 272, 453
CseI GACGC 2 cut(s) 20, 742
CsiI ACCWGGT 1 cut(s) 429
Csp6I GTAC 3 cut(s) 422, 801, 875
CviAII CATG 1 cut(s) 266
CviQI GTAC 3 cut(s) 422, 801, 875
DdeI CTNAG 3 cut(s) 323, 932, 984
DpnI GATC 3 cut(s) 68, 154, 647
DpnII GATC 3 cut(s) 66, 152, 645
DrdI GACNNNNNNGTC 1 cut(s) 131
DriI GACNNNNNGTC 1 cut(s) 310
DseDI GACNNNNNNGTC 1 cut(s) 131
EaeI YGGCCR 1 cut(s) 298
Eam1105I GACNNNNNGTC 1 cut(s) 310
EciI GGCGGA 1 cut(s) 254
Eco130I CCWWGG 1 cut(s) 456
Eco24I GRGCYC 1 cut(s) 103
Eco31I GGTCTC 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 453
EcoO109I RGGNCCY 2 cut(s) 272, 453
EcoRI GAATTC 1 cut(s) 345
EcoRII CCWGG 2 cut(s) 95, 429
EcoT14I CCWWGG 1 cut(s) 456
EcoT22I ATGCAT 1 cut(s) 848
EcoT38I GRGCYC 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 456
Esp3I CGTCTC 1 cut(s) 190
FaeI CATG 1 cut(s) 269
FalI AAGNNNNNCTT 2 cut(s) 440, 472
FaqI GGGAC 3 cut(s) 38, 347, 682
FatI CATG 1 cut(s) 265
FauNDI CATATG 1 cut(s) 280
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 4 cut(s) 7, 320, 531, 1096
FokI GGATG 6 cut(s) 158, 366, 572, 677, 893, 1067
FriOI GRGCYC 1 cut(s) 103
Fsp4HI GCNGC 4 cut(s) 7, 320, 531, 1096
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 264
GluI GCNGC 4 cut(s) 7, 320, 531, 1096
GsaI CCCAGC 1 cut(s) 559
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 2 cut(s) 274, 300
HgaI GACGC 2 cut(s) 20, 742
HhaI GCGC 1 cut(s) 265
Hin1II CATG 1 cut(s) 269
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HinfI GANTC 4 cut(s) 311, 436, 539, 958
HphI GGTGA 3 cut(s) 170, 445, 967
Hpy166II GTNNAC 2 cut(s) 374, 1086
Hpy188I TCNGA 7 cut(s) 89, 382, 604, 645, 661, 810, 841
Hpy188III TCNNGA 2 cut(s) 479, 770
Hpy8I GTNNAC 2 cut(s) 374, 1086
Hpy99I CGWCG 5 cut(s) 14, 137, 140, 308, 386
HpyAV CCTTC 2 cut(s) 358, 839
HpyCH4III ACNGT 2 cut(s) 82, 378
HpyCH4IV ACGT 3 cut(s) 384, 676, 1088
HpyF10VI GCNNNNNNNGC 6 cut(s) 271, 759, 773, 917, 1083, 1092
HpyF3I CTNAG 3 cut(s) 323, 932, 984
HpySE526I ACGT 3 cut(s) 384, 676, 1088
Hsp92II CATG 1 cut(s) 269
HspAI GCGC 1 cut(s) 263
Kzo9I GATC 3 cut(s) 66, 152, 645
LmnI GCTCC 5 cut(s) 58, 106, 198, 226, 917
Lsp1109I GCAGC 3 cut(s) 306, 517, 1082
LweI GCATC 3 cut(s) 763, 878, 1022
MabI ACCWGGT 1 cut(s) 429
MaeI CTAG 1 cut(s) 200
MaeII ACGT 3 cut(s) 384, 676, 1088
MaeIII GTNAC 1 cut(s) 1021
MalI GATC 3 cut(s) 68, 154, 647
MboI GATC 3 cut(s) 66, 152, 645
MboII GAAGA 2 cut(s) 41, 153
MflI RGATCY 2 cut(s) 66, 152
MhlI GDGCHC 3 cut(s) 103, 982, 1088
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 6 cut(s) 247, 290, 345, 474, 582, 737
MluNI TGGCCA 1 cut(s) 300
MlyI GAGTC 1 cut(s) 320
MmeI TCCRAC 3 cut(s) 405, 788, 895
Mox20I TGGCCA 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 848
MroXI GAANNNNTTC 1 cut(s) 48
MscI TGGCCA 1 cut(s) 300
MseI TTAA 6 cut(s) 111, 258, 288, 591, 725, 1108
MslI CAYNNNNRTG 1 cut(s) 679
Msp20I TGGCCA 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 97, 431
Mva1269I GAATGC 3 cut(s) 598, 613, 848
MvaI CCWGG 2 cut(s) 97, 431
MwoI GCNNNNNNNGC 6 cut(s) 271, 759, 773, 917, 1083, 1092
NdeI CATATG 1 cut(s) 280
NdeII GATC 3 cut(s) 66, 152, 645
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 6 cut(s) 102, 154, 243, 273, 454, 827
NmuCI GTSAC 1 cut(s) 1021
NsiI ATGCAT 1 cut(s) 848
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 3 cut(s) 598, 613, 848
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 3 cut(s) 436, 539, 958
PkrI GCNGC 4 cut(s) 8, 321, 532, 1097
PleI GAGTC 1 cut(s) 319
PpsI GAGTC 1 cut(s) 319
Ppu21I YACGTR 1 cut(s) 677
PpuMI RGGWCCY 1 cut(s) 453
PshAI GACNNNNGTC 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 453
Psp6I CCWGG 2 cut(s) 95, 429
PspFI CCCAGC 1 cut(s) 555
PspGI CCWGG 2 cut(s) 95, 429
PspN4I GGNNCC 6 cut(s) 102, 154, 243, 273, 454, 827
PspPI GGNCC 2 cut(s) 272, 453
PspPPI RGGWCCY 1 cut(s) 453
PsuI RGATCY 2 cut(s) 66, 152
RsaI GTAC 3 cut(s) 423, 802, 876
RsaNI GTAC 3 cut(s) 422, 801, 875
RseI CAYNNNNRTG 1 cut(s) 679
SaqAI TTAA 6 cut(s) 111, 258, 288, 591, 725, 1108
SatI GCNGC 4 cut(s) 7, 320, 531, 1096
Sau3AI GATC 3 cut(s) 66, 152, 645
Sau96I GGNCC 2 cut(s) 272, 453
ScaI AGTACT 1 cut(s) 423
SchI GAGTC 1 cut(s) 320
ScrFI CCNGG 2 cut(s) 97, 431
SduI GDGCHC 3 cut(s) 103, 982, 1088
SexAI ACCWGGT 1 cut(s) 429
SfaNI GCATC 3 cut(s) 763, 878, 1022
SfcI CTRYAG 1 cut(s) 37
SinI GGWCC 1 cut(s) 453
SmiMI CAYNNNNRTG 1 cut(s) 679
Sse9I AATT 6 cut(s) 247, 290, 345, 474, 582, 737
SsiI CCGC 3 cut(s) 6, 93, 239
SspI AATATT 1 cut(s) 906
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 2 cut(s) 95, 429
StyI CCWWGG 1 cut(s) 456
TaaI ACNGT 2 cut(s) 82, 378
TaiI ACGT 3 cut(s) 387, 679, 1091
TaqI TCGA 2 cut(s) 343, 349
TasI AATT 6 cut(s) 247, 290, 345, 474, 582, 737
TatI WGTACW 2 cut(s) 421, 874
TauI GCSGC 1 cut(s) 9
TfiI GAWTC 3 cut(s) 436, 539, 958
Tru1I TTAA 6 cut(s) 111, 258, 288, 591, 725, 1108
Tru9I TTAA 6 cut(s) 111, 258, 288, 591, 725, 1108
TseFI GTSAC 1 cut(s) 1021
TseI GCWGC 3 cut(s) 319, 530, 1095
Tsp45I GTSAC 1 cut(s) 1021
TspDTI ATGAA 2 cut(s) 383, 909
TspGWI ACGGA 1 cut(s) 145
VneI GTGCAC 1 cut(s) 1084
VpaK11BI GGWCC 1 cut(s) 453
XapI RAATTY 2 cut(s) 290, 345
XmiI GTMKAC 1 cut(s) 373
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 1 cut(s) 200
ZrmI AGTACT 1 cut(s) 423
Zsp2I ATGCAT 1 cut(s) 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.