Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0012493

Physical Location & Seq

Genomic Coordinates Reverse (-)

7310967 .. 7313789

Gene Structure 2823 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG089100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1113 bp

ATGTGCAGCGACGCAAGGAACTGGGACGAAGAAGCCTACAGGGAAACCATTCTAAAGGAGCGAGAGATCCAAACCCGAACCGTTTTCCGAACCGCCTGGGCTCCCTCCCTTAACCCTAACCCCGACACCATCGTCGTCGCCTCCAGCGATGGATCCGTTTCTTCCTACTCCATCCCTCACCTCGTCTCCAAGCTCCCACTAGGTTTCAGCAATGTGAAAGCTCCATACTTGTTAATGGCGGAACCTAATTGCTTCCTTAAAGCGCATGAGGGGCCTGCATATGACGTAAAATTTTATGGCCACGGGGGCGAGGAGTCTATGCTGCTAAGCTGCGGGGATGATGGTCGAATTCGAGGATGGAGGTGGAAGGATTGTATACATTCCGACGTGCCTATTCATTTGCAAGGAGACCAAGTGAAGCCAGTACTTGACCTGGTGAATCCTCAACATAAGGGTCCTTGGGGGGCTTTATCTCCAATTCCTGAAAACAATGCTCTTGCTGTTGATAATCAAGGAGGGTCCATTTTCTCTGCTGCTGGCGATTCTTGCGTCTATTGCTGGGATGTGGAAACAGGTCAAGTCAAAATGGTTTTTAAGGGGCACTCAGATTACTTGCATTCCATAGTCGCCCGAAACTCTACAAATCAGATCATAACTGGTTCAGAGGATGGGACGGCACGTATATGGGATTGCAAAAGTGGAAAGGCAGTTCAAGTGATTGAGCCATTAAAGAATAATAAATTGAAAGGGTCGTGCGTCAGTTGCATTGCTCTTGATGCAAGTGAAAGCTGGTTGGCTTGTGGTACTGGTCGGAGTTTATCAGTTTGGAACCTTCCTGCATCTGAATGCATTTCAAGGACTTCAACTCGTGCATCTGTACAGGATGTAGTATTTGATGAAAATCAAATATTAGCAGTTGGAGCAGAGCCTCTACTTAGCCGTTTTGACATAAATGGGGTGATTCTTTCACAAATGCAATGTGCCCCTCAGTCAGCCTTTTCTGTCTCATTACACACATCTGGGGTTACAGCAATAGGAGGTTATGGAGGAGTTGTGGATGTTATTTCCCAGTTCGGAAGCCATTTGTGCACGTTTGGCTGCAAATGTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006464 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009787 GO:0009788 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010646 GO:0010648 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016567 GO:0019222 GO:0019538 GO:0023051 GO:0023057 GO:0030422 GO:0031047 GO:0031050 GO:0031461 GO:0032446 GO:0032991 GO:0034641 GO:0035194 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070647 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0080008 GO:0090304 GO:1901360 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901699 GO:1902494 GO:1905957 GO:1905958 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

WD40

Protein Analysis

370

Amino Acids

39.99

Weight (kDa)

5.57

Isoelectric Point (pI)

38.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR41	PF25178	141 - 288	4.4e-13	WDR41
Beta-prop_THOC3	PF25174	164 - 307	2.7e-13	THOC3 beta-propeller domain
WD40_Prp19	PF24814	164 - 292	9.2e-13	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	164 - 244	1.6e-07	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	165 - 277	3.1e-13	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	165 - 301	2.4e-14	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	165 - 269	3.4e-06	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	166 - 286	1.4e-11	WDR55
WD40_CDC20-Fz	PF24807	168 - 302	1.3e-10	CDC20/Fizzy WD40 domain
EIF3I	PF24805	169 - 241	7.6e-09	EIF3I
WD40_Gbeta	PF25391	174 - 239	1.6e-08	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	175 - 232	6.3e-06	TEP-1 second beta-propeller
WD40	PF00400	193 - 230	8.2e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012493)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G19430
fragaria_vesca	FvH4_1g07921
malus_domestica	MD02G1082800.v1.1 MD15G1210900.v1.1
prunus_persica	Prupe.7G205900_v2.0.a1 Prupe.7G205900_v2.0.a1
pyrus_communis	pycom02g06570 pycom15g18680
rosa_chinensis	RchiOBHm_Chr2g0093801
rosa_multiflora	Rmu_co8487551.1_g000001 Rmu_sc0017523.1_g000001
rosa_roxburghii	Rroxscaffold_2G00147790
rosa_rugosa	Rorug05G0508300
rosa_samantha	Rh2AG086400 Rh2BG086700 Rh2CG089100 Rh2DG084900
rosa_wichuraiana	Rw5G017310 Rw6G040860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	131
AccI	GTMKAC	1 cut(s)	376
AciI	CCGC	3 cut(s)	93, 239, 333
AclWI	GGATC	3 cut(s)	61, 147, 160
AcoI	YGGCCR	1 cut(s)	298
AcsI	RAATTY	2 cut(s)	290, 348
AfaI	GTAC	3 cut(s)	426, 805, 879
AgsI	TTSAA	4 cut(s)	713, 745, 855, 864
AjiI	CACGTC	1 cut(s)	388
AjnI	CCWGG	2 cut(s)	95, 432
AloI	GAACNNNNNNTCC	2 cut(s)	693, 725
AluBI	AGCT	4 cut(s)	193, 221, 330, 789
AluI	AGCT	4 cut(s)	193, 221, 330, 789
Alw21I	GWGCWC	1 cut(s)	1091
Alw26I	GTCTC	3 cut(s)	190, 402, 1009
Alw44I	GTGCAC	1 cut(s)	1087
AlwI	GGATC	3 cut(s)	61, 147, 160
AoxI	GGCC	2 cut(s)	272, 298
ApaLI	GTGCAC	1 cut(s)	1087
ApeKI	GCWGC	5 cut(s)	6, 322, 330, 533, 1098
ApoI	RAATTY	2 cut(s)	290, 348
Asp700I	GAANNNNTTC	1 cut(s)	48
AspLEI	GCGC	1 cut(s)	265
AspS9I	GGNCC	3 cut(s)	272, 455, 519
AsuHPI	GGTGA	3 cut(s)	170, 448, 970
AvaII	GGWCC	2 cut(s)	455, 519
BaeGI	GKGCMC	3 cut(s)	603, 985, 1091
BalI	TGGCCA	1 cut(s)	300
BamHI	GGATCC	1 cut(s)	152
BanII	GRGCYC	1 cut(s)	103
BauI	CACGAG	1 cut(s)	867
Bbv12I	GWGCWC	1 cut(s)	1091
BbvI	GCAGC	5 cut(s)	18, 309, 317, 520, 1085
BccI	CCATC	6 cut(s)	137, 143, 179, 335, 351, 662
BceAI	ACGGC	2 cut(s)	690, 924
BciT130I	CCWGG	2 cut(s)	97, 434
BcoDI	GTCTC	3 cut(s)	190, 402, 1009
BfaI	CTAG	1 cut(s)	200
BfmI	CTRYAG	1 cut(s)	37
BglI	GCCNNNNNGGC	1 cut(s)	306
BisI	GCNGC	5 cut(s)	7, 323, 331, 534, 1099
BlpI	GCTNAGC	1 cut(s)	326
BlsI	GCNGC	5 cut(s)	8, 324, 332, 535, 1100
BmcAI	AGTACT	1 cut(s)	426
Bme1390I	CCNGG	2 cut(s)	97, 434
Bme18I	GGWCC	2 cut(s)	455, 519
BmgBI	CACGTC	1 cut(s)	388
BmgT120I	GGNCC	3 cut(s)	272, 455, 519
BmiI	GGNNCC	7 cut(s)	102, 154, 243, 273, 456, 520, 830
BmrFI	CCNGG	2 cut(s)	97, 434
BmrI	ACTGGG	2 cut(s)	31, 1063
BmsI	GCATC	3 cut(s)	766, 848, 881
BmuI	ACTGGG	2 cut(s)	31, 1063
BpmI	CTGGAG	1 cut(s)	127
Bpu1102I	GCTNAGC	1 cut(s)	326
BsaAI	YACGTR	1 cut(s)	680
BsaBI	GATNNNNATC	1 cut(s)	900
BsaI	GGTCTC	1 cut(s)	402
BsaJI	CCNNGG	3 cut(s)	96, 301, 458
BsaXI	ACNNNNNCTCC	6 cut(s)	170, 200, 399, 429, 1038, 1068
Bse1I	ACTGG	5 cut(s)	26, 422, 661, 811, 1069
Bse3DI	GCAATG	3 cut(s)	217, 765, 983
Bse8I	GATNNNNATC	1 cut(s)	900
BseBI	CCWGG	2 cut(s)	97, 434
BseDI	CCNNGG	3 cut(s)	96, 301, 458
BseGI	GGATG	7 cut(s)	171, 343, 362, 568, 673, 889, 1063
BseJI	GATNNNNATC	1 cut(s)	900
BseMI	GCAATG	3 cut(s)	217, 765, 983
BseMII	CTCAG	2 cut(s)	618, 1001
BseNI	ACTGG	5 cut(s)	26, 422, 661, 811, 1069
BseRI	GAGGAG	2 cut(s)	326, 1062
BseSI	GKGCMC	3 cut(s)	603, 985, 1091
BseXI	GCAGC	5 cut(s)	18, 309, 317, 520, 1085
BseYI	CCCAGC	1 cut(s)	558
BsgI	GTGCAG	1 cut(s)	25
BshFI	GGCC	2 cut(s)	274, 300
BsiHKAI	GWGCWC	1 cut(s)	1091
BslFI	GGGAC	2 cut(s)	38, 685
BsmAI	GTCTC	3 cut(s)	190, 402, 1009
BsmBI	CGTCTC	1 cut(s)	190
BsmFI	GGGAC	2 cut(s)	38, 685
BsmI	GAATGC	2 cut(s)	616, 851
BsnI	GGCC	2 cut(s)	274, 300
Bso31I	GGTCTC	1 cut(s)	402
Bsp1286I	GDGCHC	4 cut(s)	103, 603, 985, 1091
Bsp1407I	TGTACA	1 cut(s)	877
Bsp143I	GATC	3 cut(s)	66, 152, 648
Bsp1720I	GCTNAGC	1 cut(s)	326
BspACI	CCGC	3 cut(s)	93, 239, 333
BspANI	GGCC	2 cut(s)	274, 300
BspCNI	CTCAG	2 cut(s)	617, 1000
BspLI	GGNNCC	7 cut(s)	102, 154, 243, 273, 456, 520, 830
BspPI	GGATC	3 cut(s)	61, 147, 160
BspTNI	GGTCTC	1 cut(s)	402
BsrDI	GCAATG	3 cut(s)	217, 765, 983
BsrGI	TGTACA	1 cut(s)	877
BsrI	ACTGG	5 cut(s)	26, 422, 661, 811, 1069
BssECI	CCNNGG	3 cut(s)	96, 301, 458
BssMI	GATC	3 cut(s)	66, 152, 648
BssNAI	GTATAC	1 cut(s)	377
BssSI	CACGAG	1 cut(s)	867
BssT1I	CCWWGG	1 cut(s)	458
Bst1107I	GTATAC	1 cut(s)	377
Bst2BI	CACGAG	1 cut(s)	867
Bst2UI	CCWGG	2 cut(s)	97, 434
Bst4CI	ACNGT	1 cut(s)	82
BstAUI	TGTACA	1 cut(s)	877
BstBAI	YACGTR	1 cut(s)	680
BstC8I	GCNNGC	2 cut(s)	276, 538
BstDEI	CTNAG	4 cut(s)	326, 604, 935, 987
BstDSI	CCRYGG	1 cut(s)	301
BstF5I	GGATG	7 cut(s)	171, 343, 362, 568, 673, 889, 1063
BstHHI	GCGC	1 cut(s)	265
BstKTI	GATC	3 cut(s)	69, 155, 651
BstMAI	GTCTC	3 cut(s)	190, 402, 1009
BstMBI	GATC	3 cut(s)	66, 152, 648
BstMWI	GCNNNNNNNGC	9 cut(s)	271, 306, 546, 555, 762, 776, 920, 1086, 1095
BstNI	CCWGG	2 cut(s)	97, 434
BstSCI	CCNGG	2 cut(s)	95, 432
BstSFI	CTRYAG	1 cut(s)	37
BstSLI	GKGCMC	3 cut(s)	603, 985, 1091
BstV1I	GCAGC	5 cut(s)	18, 309, 317, 520, 1085
BstX2I	RGATCY	2 cut(s)	66, 152
BstYI	RGATCY	2 cut(s)	66, 152
BstZ17I	GTATAC	1 cut(s)	377
BsuRI	GGCC	2 cut(s)	274, 300
BtgI	CCRYGG	1 cut(s)	301
BtgZI	GCGATG	1 cut(s)	162
BtrI	CACGTC	1 cut(s)	388
BtsCI	GGATG	7 cut(s)	171, 343, 362, 568, 673, 889, 1063
Cac8I	GCNNGC	2 cut(s)	276, 538
CfoI	GCGC	1 cut(s)	265
Cfr13I	GGNCC	3 cut(s)	272, 455, 519
CseI	GACGC	3 cut(s)	20, 538, 745
CsiI	ACCWGGT	1 cut(s)	432
Csp6I	GTAC	3 cut(s)	425, 804, 878
CviAII	CATG	1 cut(s)	266
CviQI	GTAC	3 cut(s)	425, 804, 878
DdeI	CTNAG	4 cut(s)	326, 604, 935, 987
DpnI	GATC	3 cut(s)	68, 154, 650
DpnII	GATC	3 cut(s)	66, 152, 648
DrdI	GACNNNNNNGTC	1 cut(s)	131
DseDI	GACNNNNNNGTC	1 cut(s)	131
EaeI	YGGCCR	1 cut(s)	298
EciI	GGCGGA	1 cut(s)	254
Eco130I	CCWWGG	1 cut(s)	458
Eco24I	GRGCYC	1 cut(s)	103
Eco31I	GGTCTC	1 cut(s)	402
Eco47I	GGWCC	2 cut(s)	455, 519
EcoO109I	RGGNCCY	2 cut(s)	272, 455
EcoRI	GAATTC	1 cut(s)	348
EcoRII	CCWGG	2 cut(s)	95, 432
EcoT14I	CCWWGG	1 cut(s)	458
EcoT22I	ATGCAT	1 cut(s)	851
EcoT38I	GRGCYC	1 cut(s)	103
ErhI	CCWWGG	1 cut(s)	458
Esp3I	CGTCTC	1 cut(s)	190
FaeI	CATG	1 cut(s)	269
FaqI	GGGAC	2 cut(s)	38, 685
FatI	CATG	1 cut(s)	265
FauI	CCCGC	1 cut(s)	326
FauNDI	CATATG	1 cut(s)	280
FblI	GTMKAC	1 cut(s)	376
Fnu4HI	GCNGC	5 cut(s)	7, 323, 331, 534, 1099
FokI	GGATG	7 cut(s)	158, 350, 369, 575, 680, 896, 1070
FriOI	GRGCYC	1 cut(s)	103
Fsp4HI	GCNGC	5 cut(s)	7, 323, 331, 534, 1099
FspBI	CTAG	1 cut(s)	200
GlaI	GCGC	1 cut(s)	264
GluI	GCNGC	5 cut(s)	7, 323, 331, 534, 1099
GsaI	CCCAGC	1 cut(s)	562
GsuI	CTGGAG	1 cut(s)	127
HaeIII	GGCC	2 cut(s)	274, 300
HgaI	GACGC	3 cut(s)	20, 538, 745
HhaI	GCGC	1 cut(s)	265
Hin1II	CATG	1 cut(s)	269
Hin6I	GCGC	1 cut(s)	263
HinP1I	GCGC	1 cut(s)	263
HinfI	GANTC	4 cut(s)	314, 439, 542, 961
HphI	GGTGA	3 cut(s)	170, 448, 970
Hpy166II	GTNNAC	2 cut(s)	377, 1089
Hpy188I	TCNGA	8 cut(s)	89, 385, 607, 648, 664, 813, 844, 1076
Hpy188III	TCNNGA	2 cut(s)	482, 773
Hpy8I	GTNNAC	2 cut(s)	377, 1089
Hpy99I	CGWCG	4 cut(s)	14, 137, 140, 389
HpyAV	CCTTC	2 cut(s)	361, 842
HpyCH4III	ACNGT	1 cut(s)	82
HpyCH4IV	ACGT	4 cut(s)	285, 387, 679, 1091
HpyF10VI	GCNNNNNNNGC	9 cut(s)	271, 306, 546, 555, 762, 776, 920, 1086, 1095
HpyF3I	CTNAG	4 cut(s)	326, 604, 935, 987
HpySE526I	ACGT	4 cut(s)	285, 387, 679, 1091
Hsp92II	CATG	1 cut(s)	269
HspAI	GCGC	1 cut(s)	263
Kzo9I	GATC	3 cut(s)	66, 152, 648
LmnI	GCTCC	5 cut(s)	58, 106, 198, 226, 920
Lsp1109I	GCAGC	5 cut(s)	18, 309, 317, 520, 1085
LweI	GCATC	3 cut(s)	766, 848, 881
MabI	ACCWGGT	1 cut(s)	432
MaeI	CTAG	1 cut(s)	200
MaeII	ACGT	4 cut(s)	285, 387, 679, 1091
MaeIII	GTNAC	1 cut(s)	1024
MalI	GATC	3 cut(s)	68, 154, 650
MboI	GATC	3 cut(s)	66, 152, 648
MboII	GAAGA	2 cut(s)	41, 153
MflI	RGATCY	2 cut(s)	66, 152
MhlI	GDGCHC	4 cut(s)	103, 603, 985, 1091
MlsI	TGGCCA	1 cut(s)	300
MluCI	AATT	5 cut(s)	247, 290, 348, 477, 740
MluNI	TGGCCA	1 cut(s)	300
MlyI	GAGTC	1 cut(s)	323
MmeI	TCCRAC	3 cut(s)	408, 791, 898
Mox20I	TGGCCA	1 cut(s)	300
Mph1103I	ATGCAT	1 cut(s)	851
MroXI	GAANNNNTTC	1 cut(s)	48
MscI	TGGCCA	1 cut(s)	300
MseI	TTAA	6 cut(s)	111, 233, 258, 594, 728, 1111
MslI	CAYNNNNRTG	2 cut(s)	682, 844
Msp20I	TGGCCA	1 cut(s)	300
MspR9I	CCNGG	2 cut(s)	97, 434
Mva1269I	GAATGC	2 cut(s)	616, 851
MvaI	CCWGG	2 cut(s)	97, 434
MwoI	GCNNNNNNNGC	9 cut(s)	271, 306, 546, 555, 762, 776, 920, 1086, 1095
NdeI	CATATG	1 cut(s)	280
NdeII	GATC	3 cut(s)	66, 152, 648
NlaIII	CATG	1 cut(s)	269
NlaIV	GGNNCC	7 cut(s)	102, 154, 243, 273, 456, 520, 830
NsiI	ATGCAT	1 cut(s)	851
PcsI	WCGNNNNNNNCGW	1 cut(s)	144
PctI	GAATGC	2 cut(s)	616, 851
PdmI	GAANNNNTTC	1 cut(s)	48
PfeI	GAWTC	3 cut(s)	439, 542, 961
PkrI	GCNGC	5 cut(s)	8, 324, 332, 535, 1100
PleI	GAGTC	1 cut(s)	322
PpsI	GAGTC	1 cut(s)	322
Ppu21I	YACGTR	1 cut(s)	680
PpuMI	RGGWCCY	1 cut(s)	455
Psp5II	RGGWCCY	1 cut(s)	455
Psp6I	CCWGG	2 cut(s)	95, 432
PspFI	CCCAGC	1 cut(s)	558
PspGI	CCWGG	2 cut(s)	95, 432
PspN4I	GGNNCC	7 cut(s)	102, 154, 243, 273, 456, 520, 830
PspPI	GGNCC	3 cut(s)	272, 455, 519
PspPPI	RGGWCCY	1 cut(s)	455
PsuI	RGATCY	2 cut(s)	66, 152
RsaI	GTAC	3 cut(s)	426, 805, 879
RsaNI	GTAC	3 cut(s)	425, 804, 878
RseI	CAYNNNNRTG	2 cut(s)	682, 844
SaqAI	TTAA	6 cut(s)	111, 233, 258, 594, 728, 1111
SatI	GCNGC	5 cut(s)	7, 323, 331, 534, 1099
Sau3AI	GATC	3 cut(s)	66, 152, 648
Sau96I	GGNCC	3 cut(s)	272, 455, 519
ScaI	AGTACT	1 cut(s)	426
SchI	GAGTC	1 cut(s)	323
ScrFI	CCNGG	2 cut(s)	97, 434
SduI	GDGCHC	4 cut(s)	103, 603, 985, 1091
SexAI	ACCWGGT	1 cut(s)	432
SfaNI	GCATC	3 cut(s)	766, 848, 881
SfcI	CTRYAG	1 cut(s)	37
SinI	GGWCC	2 cut(s)	455, 519
SmiMI	CAYNNNNRTG	2 cut(s)	682, 844
Sse9I	AATT	5 cut(s)	247, 290, 348, 477, 740
SsiI	CCGC	3 cut(s)	93, 239, 333
SspI	AATATT	1 cut(s)	909
SspMI	CTAG	1 cut(s)	200
StyD4I	CCNGG	2 cut(s)	95, 432
StyI	CCWWGG	1 cut(s)	458
TaaI	ACNGT	1 cut(s)	82
TaiI	ACGT	4 cut(s)	288, 390, 682, 1094
TaqI	TCGA	2 cut(s)	346, 352
TasI	AATT	5 cut(s)	247, 290, 348, 477, 740
TatI	WGTACW	2 cut(s)	424, 877
TfiI	GAWTC	3 cut(s)	439, 542, 961
Tru1I	TTAA	6 cut(s)	111, 233, 258, 594, 728, 1111
Tru9I	TTAA	6 cut(s)	111, 233, 258, 594, 728, 1111
TseI	GCWGC	5 cut(s)	6, 322, 330, 533, 1098
TspDTI	ATGAA	2 cut(s)	386, 912
TspGWI	ACGGA	1 cut(s)	145
VneI	GTGCAC	1 cut(s)	1087
VpaK11BI	GGWCC	2 cut(s)	455, 519
XapI	RAATTY	2 cut(s)	290, 348
XmiI	GTMKAC	1 cut(s)	376
XmnI	GAANNNNTTC	1 cut(s)	48
XspI	CTAG	1 cut(s)	200
ZrmI	AGTACT	1 cut(s)	426
Zsp2I	ATGCAT	1 cut(s)	851

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.