MD02G1095400.v1.1
ERF Family

Cupin superfamily (DUF985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013260
Physical Location & Seq
Reverse (-)
7538970 .. 7540902
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1095400.v1.1.491

Sequence Viewer

Length: 693 bp
ATGTCGGTTTGTCAAATCGCCAAAACCTACCACATGTTAAAGAGGTACCAAACACAGATCACAATCGAATATAGTGGCTGTTGTCAGAGGAAGACGAAGAGTTTAAAGATCCCAGGAAGGAACATGGGAACTGCATCAGAGATTGTAGCTAAACTGAAACTGAATCCCCACCCTGAAGGTGGCTTTTATTTCGAAACTTTCAGAGATACTTCCATTGCCCTGCCATTGTGTCAGCTTCCCCCAGACTACAAGGTTGAGCGCCCTGTGAGTTCATCTATCTACTTCTTGCTGCCGTCTGGGAGTGTATCTCGCCTTCATCGTATACCAATGGCAGAAACCTGGCATTTTTATCTCGGAGAGCCCATTATGGTCGTGGAGTTGGGGGAAGATGGGAAGGTCAAGTTAACCTGCCTCGGACCCGATTTGGGAGCGGATCAGCAACCCCAGTACACAGTGCCTCCAAATGTATGGTTTGGTTCCTTCCCAACAAAGGACTACAGCATTTCATCAGAAGGGGCATTGCTCAAAGCTGCTCCAAGGGATGCCGAGACTCACTACTCTCTCGTGGGATGTACTTGTGCACCTGCCTTTCAGTTCCAGGATTTCGAGTTGGCAAAACGCTCCGATCTTCTTCCTCTTTTTCCTGGTTACGAGGCTCTCATAACGCTGCTTACTTTCCCTGAAGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_5

Protein Analysis

231

Amino Acids

25.71

Weight (kDa)

6.21

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_5 PF06172 47 - 205 4.1e-43 Cupin superfamily (DUF985)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19130
fragaria_vesca FvH4_1g09020
malus_domestica MD02G1095400.v1.1
prunus_persica Prupe.7G195700_v2.0.a1
pyrus_communis pycom02g07550
rosa_chinensis RchiOBHm_Chr2g0095441
rosa_laevigata RLG00000016566
rosa_multiflora Rmu_co8132630.1_g000001 Rmu_co8397589.1_g000001 Rmu_sc0010847.1_g000003 Rmu_ssc0000183.1_g000046
rosa_roxburghii Rroxscaffold_2G00146370
rosa_rugosa Rorug02G0051100
rosa_samantha Rh2AG099100 Rh2BG100300 Rh2CG102500 Rh2DG100000
rosa_wichuraiana Rw2G007500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 592
Acc36I ACCTGC 2 cut(s) 416, 592
Acc65I GGTACC 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 45
AccBSI CCGCTC 1 cut(s) 431
AccI GTMKAC 1 cut(s) 322
AciI CCGC 1 cut(s) 431
AclWI GGATC 2 cut(s) 103, 441
AcuI CTGAAG 1 cut(s) 195
AfaI GTAC 3 cut(s) 47, 449, 574
AfiI CCNNNNNNNGG 3 cut(s) 179, 425, 490
AflIII ACRYGT 1 cut(s) 33
AjnI CCWGG 4 cut(s) 112, 338, 597, 643
AluBI AGCT 3 cut(s) 149, 235, 530
AluI AGCT 3 cut(s) 149, 235, 530
Alw21I GWGCWC 1 cut(s) 583
Alw26I GTCTC 1 cut(s) 542
Alw44I GTGCAC 1 cut(s) 579
AlwI GGATC 2 cut(s) 103, 441
ApaLI GTGCAC 1 cut(s) 579
ApeKI GCWGC 3 cut(s) 289, 530, 667
Asp718I GGTACC 1 cut(s) 45
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 1 cut(s) 416
AsuII TTCGAA 1 cut(s) 192
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 583
BanI GGYRCC 1 cut(s) 45
BanII GRGCYC 1 cut(s) 363
BarI GAAGNNNNNNTAC 2 cut(s) 297, 329
BauI CACGAG 1 cut(s) 563
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 583
BbvI GCAGC 3 cut(s) 276, 517, 654
BccI CCATC 1 cut(s) 383
BceAI ACGGC 1 cut(s) 277
BciT130I CCWGG 4 cut(s) 114, 340, 599, 645
BcoDI GTCTC 1 cut(s) 542
BfmI CTRYAG 1 cut(s) 496
BfoI RGCGCY 1 cut(s) 262
BfuAI ACCTGC 2 cut(s) 416, 592
BisI GCNGC 3 cut(s) 290, 531, 668
BlsI GCNGC 3 cut(s) 291, 532, 669
Bme1390I CCNGG 4 cut(s) 114, 340, 599, 645
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 3 cut(s) 47, 418, 478
BmrFI CCNGG 4 cut(s) 114, 340, 599, 645
BmrI ACTGGG 1 cut(s) 439
BmsI GCATC 2 cut(s) 143, 532
BmuI ACTGGG 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 98
BplI GAGNNNNNCTC 2 cut(s) 292, 324
Bpu14I TTCGAA 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 112, 412, 536
BsaXI ACNNNNNCTCC 2 cut(s) 442, 472
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 425, 490
Bse1I ACTGG 1 cut(s) 445
Bse3DI GCAATG 2 cut(s) 213, 518
Bse8I GATNNNNATC 1 cut(s) 62
BseBI CCWGG 4 cut(s) 114, 340, 599, 645
BseDI CCNNGG 3 cut(s) 112, 412, 536
BseGI GGATG 2 cut(s) 547, 575
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 3 cut(s) 179, 425, 490
BseMI GCAATG 2 cut(s) 213, 518
BseNI ACTGG 1 cut(s) 445
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 3 cut(s) 276, 517, 654
BshNI GGYRCC 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 583
BslI CCNNNNNNNGG 3 cut(s) 179, 425, 490
BsmAI GTCTC 1 cut(s) 542
Bsp119I TTCGAA 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 363, 583
Bsp143I GATC 4 cut(s) 57, 108, 433, 625
BspACI CCGC 1 cut(s) 431
BspLI GGNNCC 3 cut(s) 47, 418, 478
BspMI ACCTGC 2 cut(s) 416, 592
BspPI GGATC 2 cut(s) 103, 441
BspT104I TTCGAA 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 45
BsrBI CCGCTC 1 cut(s) 431
BsrDI GCAATG 2 cut(s) 213, 518
BsrI ACTGG 1 cut(s) 445
BssECI CCNNGG 3 cut(s) 112, 412, 536
BssMI GATC 4 cut(s) 57, 108, 433, 625
BssNAI GTATAC 1 cut(s) 323
BssSI CACGAG 1 cut(s) 563
BssT1I CCWWGG 1 cut(s) 536
Bst1107I GTATAC 1 cut(s) 323
Bst2BI CACGAG 1 cut(s) 563
Bst2UI CCWGG 4 cut(s) 114, 340, 599, 645
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 1 cut(s) 92
BstBI TTCGAA 1 cut(s) 192
BstF5I GGATG 2 cut(s) 547, 575
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 4 cut(s) 60, 111, 436, 628
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 4 cut(s) 57, 108, 433, 625
BstNI CCWGG 4 cut(s) 114, 340, 599, 645
BstNSI RCATGY 1 cut(s) 37
BstSCI CCNGG 4 cut(s) 112, 338, 597, 643
BstSFI CTRYAG 1 cut(s) 496
BstSLI GKGCMC 1 cut(s) 583
BstV1I GCAGC 3 cut(s) 276, 517, 654
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 108
BstXI CCANNNNNNTGG 1 cut(s) 468
BstYI RGATCY 1 cut(s) 108
BstZ17I GTATAC 1 cut(s) 323
BtsCI GGATG 2 cut(s) 547, 575
BtsIMutI CAGTG 1 cut(s) 459
BveI ACCTGC 2 cut(s) 416, 592
CfoI GCGC 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 416
Csp6I GTAC 3 cut(s) 46, 448, 573
CviAII CATG 2 cut(s) 34, 124
CviJI RGCY 7 cut(s) 78, 149, 183, 235, 361, 530, 656
CviKI_1 RGCY 7 cut(s) 78, 149, 183, 235, 361, 530, 656
CviQI GTAC 3 cut(s) 46, 448, 573
DpnI GATC 4 cut(s) 59, 110, 435, 627
DpnII GATC 4 cut(s) 57, 108, 433, 625
DraI TTTAAA 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 536
Eco24I GRGCYC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 195
EcoRII CCWGG 4 cut(s) 112, 338, 597, 643
EcoT14I CCWWGG 1 cut(s) 536
EcoT38I GRGCYC 1 cut(s) 363
ErhI CCWWGG 1 cut(s) 536
FaeI CATG 2 cut(s) 37, 127
FaiI YATR 8 cut(s) 35, 72, 125, 323, 368, 469, 662, 691
FatI CATG 2 cut(s) 33, 123
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 3 cut(s) 290, 531, 668
FokI GGATG 2 cut(s) 554, 582
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 3 cut(s) 290, 531, 668
GlaI GCGC 1 cut(s) 260
GluI GCNGC 3 cut(s) 290, 531, 668
HaeII RGCGCY 1 cut(s) 262
HhaI GCGC 1 cut(s) 261
Hin1II CATG 2 cut(s) 37, 127
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HinfI GANTC 2 cut(s) 163, 550
HpaI GTTAAC 1 cut(s) 405
Hpy166II GTNNAC 4 cut(s) 323, 405, 450, 581
Hpy188I TCNGA 7 cut(s) 87, 139, 203, 356, 416, 511, 625
Hpy8I GTNNAC 4 cut(s) 323, 405, 450, 581
HpyAV CCTTC 6 cut(s) 111, 170, 323, 388, 490, 506
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4V TGCA 2 cut(s) 134, 581
Hsp92II CATG 2 cut(s) 37, 127
HspAI GCGC 1 cut(s) 259
KpnI GGTACC 1 cut(s) 49
KspAI GTTAAC 1 cut(s) 405
Kzo9I GATC 4 cut(s) 57, 108, 433, 625
LmnI GCTCC 3 cut(s) 428, 538, 626
Lsp1109I GCAGC 3 cut(s) 276, 517, 654
LweI GCATC 2 cut(s) 143, 532
MaeIII GTNAC 1 cut(s) 647
MalI GATC 4 cut(s) 59, 110, 435, 627
MbiI CCGCTC 1 cut(s) 431
MboI GATC 4 cut(s) 57, 108, 433, 625
MboII GAAGA 5 cut(s) 103, 109, 398, 620, 623
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 2 cut(s) 363, 583
MlyI GAGTC 1 cut(s) 544
MnlI CCTC 6 cut(s) 36, 81, 422, 468, 645, 646
MseI TTAA 3 cut(s) 38, 104, 404
MspR9I CCNGG 4 cut(s) 114, 340, 599, 645
MvaI CCWGG 4 cut(s) 114, 340, 599, 645
NdeII GATC 4 cut(s) 57, 108, 433, 625
NlaIII CATG 2 cut(s) 37, 127
NlaIV GGNNCC 3 cut(s) 47, 418, 478
NmeAIII GCCGAG 1 cut(s) 571
NspI RCATGY 1 cut(s) 37
NspV TTCGAA 1 cut(s) 192
PaqCI CACCTGC 1 cut(s) 592
PciI ACATGT 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 316
PfeI GAWTC 1 cut(s) 163
PfoI TCCNGGA 1 cut(s) 597
PkrI GCNGC 3 cut(s) 291, 532, 669
PleI GAGTC 1 cut(s) 544
PpsI GAGTC 1 cut(s) 544
PscI ACATGT 1 cut(s) 33
Psp6I CCWGG 4 cut(s) 112, 338, 597, 643
PspGI CCWGG 4 cut(s) 112, 338, 597, 643
PspN4I GGNNCC 3 cut(s) 47, 418, 478
PspPI GGNCC 1 cut(s) 416
PsuI RGATCY 1 cut(s) 108
RsaI GTAC 3 cut(s) 47, 449, 574
RsaNI GTAC 3 cut(s) 46, 448, 573
SaqAI TTAA 3 cut(s) 38, 104, 404
SatI GCNGC 3 cut(s) 290, 531, 668
Sau3AI GATC 4 cut(s) 57, 108, 433, 625
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 1 cut(s) 544
ScrFI CCNGG 4 cut(s) 114, 340, 599, 645
SduI GDGCHC 2 cut(s) 363, 583
SfaNI GCATC 2 cut(s) 143, 532
SfcI CTRYAG 1 cut(s) 496
SfuI TTCGAA 1 cut(s) 192
SinI GGWCC 1 cut(s) 416
SsiI CCGC 1 cut(s) 431
StyD4I CCNGG 4 cut(s) 112, 338, 597, 643
StyI CCWWGG 1 cut(s) 536
TaaI ACNGT 1 cut(s) 454
TaqI TCGA 3 cut(s) 66, 192, 606
TatI WGTACW 2 cut(s) 447, 572
TfiI GAWTC 1 cut(s) 163
Tru1I TTAA 3 cut(s) 38, 104, 404
Tru9I TTAA 3 cut(s) 38, 104, 404
TscAI CASTG 1 cut(s) 459
TseI GCWGC 3 cut(s) 289, 530, 667
TspDTI ATGAA 3 cut(s) 261, 305, 495
TspRI CASTG 1 cut(s) 459
VneI GTGCAC 1 cut(s) 579
VpaK11BI GGWCC 1 cut(s) 416
XceI RCATGY 1 cut(s) 37
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 370
XmiI GTMKAC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.