Rh2DG100000
ERF Family

Cupin superfamily (DUF985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013260
Physical Location & Seq
Reverse (-)
8027321 .. 8038095
10775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG100000.1

Sequence Viewer

Length: 477 bp
ATGATCAGATTGAGTAATGAAAATGACAAGGTTGATCGCCCTGTGAGTACAGCTATTTACTTCTTGCTGCCAACTGGGGATGTTTCGCGCCTTCATCGGATACCGTGTGCAGAAACATGGCATTTCTACCTGGGAGAACCTCTTACGGTGATGGAGTTGAATGAGGAAGATGGGAAAGTCAAACTAACAAACATTGGAGCAAATTTCATTGGAGAGGATCAGGTACCCCAGTACACAGTGCCTCCAAATGTTTGGTTTGGTGCATTTCCTACAAAGGACTTCAACATTTCACCAGATGGGGGGGTGACCAAAGCTGCTCCAAGGGATTCTGAGACCCACTACTCTCTCGTGGGATGCACTTGTGCACCAGCCTTTCAGTTCCAGGATTTTGAGTTGGCAAAACGCTCAGAGCTCATTAAGCACTTCCCTGATAGGGAGCCTCTTATAATATTGCTAACAGATCCTGAAAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_5

Protein Analysis

158

Amino Acids

17.78

Weight (kDa)

4.93

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_5 PF06172 7 - 133 5.4e-32 Cupin superfamily (DUF985)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19130
fragaria_vesca FvH4_1g09020
malus_domestica MD02G1095400.v1.1
prunus_persica Prupe.7G195700_v2.0.a1
pyrus_communis pycom02g07550
rosa_chinensis RchiOBHm_Chr2g0095441
rosa_laevigata RLG00000016566
rosa_multiflora Rmu_co8132630.1_g000001 Rmu_co8397589.1_g000001 Rmu_sc0010847.1_g000003 Rmu_ssc0000183.1_g000046
rosa_roxburghii Rroxscaffold_2G00146370
rosa_rugosa Rorug02G0051100
rosa_samantha Rh2AG099100 Rh2BG100300 Rh2CG102500 Rh2DG100000
rosa_wichuraiana Rw2G007500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
Acc65I GGTACC 1 cut(s) 223
AccB1I GGYRCC 1 cut(s) 223
AccII CGCG 1 cut(s) 88
AclWI GGATC 2 cut(s) 225, 455
AcsI RAATTY 1 cut(s) 202
AfaI GTAC 3 cut(s) 49, 225, 233
AfiI CCNNNNNNNGG 2 cut(s) 299, 433
AgsI TTSAA 2 cut(s) 160, 283
AjnI CCWGG 2 cut(s) 129, 381
AluBI AGCT 3 cut(s) 53, 314, 412
AluI AGCT 3 cut(s) 53, 314, 412
Alw21I GWGCWC 2 cut(s) 367, 414
Alw26I GTCTC 1 cut(s) 326
Alw44I GTGCAC 1 cut(s) 363
AlwI GGATC 2 cut(s) 225, 455
AlwNI CAGNNNCTG 1 cut(s) 464
ApaLI GTGCAC 1 cut(s) 363
ApeKI GCWGC 2 cut(s) 67, 314
ApoI RAATTY 1 cut(s) 202
Asp718I GGTACC 1 cut(s) 223
AspLEI GCGC 1 cut(s) 90
AsuHPI GGTGA 3 cut(s) 160, 282, 316
BaeGI GKGCMC 1 cut(s) 367
BaeI ACNNNNGTAYC 2 cut(s) 215, 248
BanI GGYRCC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 414
BauI CACGAG 1 cut(s) 347
Bbv12I GWGCWC 2 cut(s) 367, 414
BbvI GCAGC 2 cut(s) 54, 301
BccI CCATC 3 cut(s) 145, 164, 290
BciT130I CCWGG 2 cut(s) 131, 383
BciVI GTATCC 1 cut(s) 93
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 326
BfuI GTATCC 1 cut(s) 93
BisI GCNGC 2 cut(s) 68, 315
BlsI GCNGC 2 cut(s) 69, 316
Bme1390I CCNGG 2 cut(s) 131, 383
BmiI GGNNCC 2 cut(s) 225, 438
BmrFI CCNGG 2 cut(s) 131, 383
BmrI ACTGGG 2 cut(s) 84, 223
BmsI GCATC 1 cut(s) 344
BmuI ACTGGG 2 cut(s) 84, 223
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 130, 320
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 2 cut(s) 299, 433
Bse1I ACTGG 2 cut(s) 79, 229
BseBI CCWGG 2 cut(s) 131, 383
BseDI CCNNGG 2 cut(s) 130, 320
BseGI GGATG 2 cut(s) 85, 359
BseLI CCNNNNNNNGG 2 cut(s) 299, 433
BseMII CTCAG 2 cut(s) 321, 420
BseNI ACTGG 2 cut(s) 79, 229
BseSI GKGCMC 1 cut(s) 367
BseXI GCAGC 2 cut(s) 54, 301
BsgI GTGCAG 1 cut(s) 129
Bsh1236I CGCG 1 cut(s) 88
BshNI GGYRCC 1 cut(s) 223
BsiHKAI GWGCWC 2 cut(s) 367, 414
BslI CCNNNNNNNGG 2 cut(s) 299, 433
BsmAI GTCTC 1 cut(s) 326
Bso31I GGTCTC 1 cut(s) 326
Bsp1286I GDGCHC 2 cut(s) 367, 414
Bsp143I GATC 4 cut(s) 3, 34, 217, 460
BspCNI CTCAG 2 cut(s) 322, 419
BspFNI CGCG 1 cut(s) 88
BspLI GGNNCC 2 cut(s) 225, 438
BspPI GGATC 2 cut(s) 225, 455
BspT107I GGYRCC 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 326
BsrI ACTGG 2 cut(s) 79, 229
BssECI CCNNGG 2 cut(s) 130, 320
BssMI GATC 4 cut(s) 3, 34, 217, 460
BssSI CACGAG 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 320
Bst2BI CACGAG 1 cut(s) 347
Bst2UI CCWGG 2 cut(s) 131, 383
Bst4CI ACNGT 3 cut(s) 105, 148, 238
BstDEI CTNAG 2 cut(s) 330, 406
BstEII GGTNACC 1 cut(s) 304
BstF5I GGATG 2 cut(s) 85, 359
BstFNI CGCG 1 cut(s) 88
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 4 cut(s) 6, 37, 220, 463
BstMAI GTCTC 1 cut(s) 326
BstMBI GATC 4 cut(s) 3, 34, 217, 460
BstMWI GCNNNNNNNGC 1 cut(s) 418
BstNI CCWGG 2 cut(s) 131, 383
BstPI GGTNACC 1 cut(s) 304
BstSCI CCNGG 2 cut(s) 129, 381
BstSLI GKGCMC 1 cut(s) 367
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 2 cut(s) 54, 301
BstX2I RGATCY 1 cut(s) 460
BstXI CCANNNNNNTGG 1 cut(s) 252
BstYI RGATCY 1 cut(s) 460
BsuI GTATCC 1 cut(s) 93
BtsCI GGATG 2 cut(s) 85, 359
BtsIMutI CAGTG 1 cut(s) 243
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 1 cut(s) 90
Csp6I GTAC 3 cut(s) 48, 224, 232
CviAII CATG 1 cut(s) 117
CviJI RGCY 5 cut(s) 53, 314, 371, 412, 439
CviKI_1 RGCY 5 cut(s) 53, 314, 371, 412, 439
CviQI GTAC 3 cut(s) 48, 224, 232
DdeI CTNAG 2 cut(s) 330, 406
DpnI GATC 4 cut(s) 5, 36, 219, 462
DpnII GATC 4 cut(s) 3, 34, 217, 460
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 1 cut(s) 320
Eco24I GRGCYC 1 cut(s) 414
Eco31I GGTCTC 1 cut(s) 326
Eco53kI GAGCTC 1 cut(s) 412
Eco91I GGTNACC 1 cut(s) 304
EcoICRI GAGCTC 1 cut(s) 412
EcoO65I GGTNACC 1 cut(s) 304
EcoRII CCWGG 2 cut(s) 129, 381
EcoT14I CCWWGG 1 cut(s) 320
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 320
FaeI CATG 1 cut(s) 120
FaiI YATR 3 cut(s) 118, 446, 475
FatI CATG 1 cut(s) 116
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 68, 315
FokI GGATG 2 cut(s) 92, 366
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 2 cut(s) 68, 315
GlaI GCGC 1 cut(s) 89
GluI GCNGC 2 cut(s) 68, 315
HhaI GCGC 1 cut(s) 90
Hin1II CATG 1 cut(s) 120
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HinfI GANTC 1 cut(s) 326
HphI GGTGA 3 cut(s) 160, 282, 316
Hpy166II GTNNAC 2 cut(s) 234, 365
Hpy188I TCNGA 4 cut(s) 8, 99, 331, 409
Hpy188III TCNNGA 1 cut(s) 464
Hpy8I GTNNAC 2 cut(s) 234, 365
HpyAV CCTTC 1 cut(s) 101
HpyCH4III ACNGT 3 cut(s) 105, 148, 238
HpyCH4V TGCA 4 cut(s) 110, 263, 357, 365
HpyF10VI GCNNNNNNNGC 1 cut(s) 418
HpyF3I CTNAG 2 cut(s) 330, 406
Hsp92II CATG 1 cut(s) 120
HspAI GCGC 1 cut(s) 88
KpnI GGTACC 1 cut(s) 227
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 4 cut(s) 3, 34, 217, 460
LmnI GCTCC 3 cut(s) 197, 322, 436
Lsp1109I GCAGC 2 cut(s) 54, 301
LweI GCATC 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 304
MalI GATC 4 cut(s) 5, 36, 219, 462
MboI GATC 4 cut(s) 3, 34, 217, 460
MboII GAAGA 1 cut(s) 179
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 2 cut(s) 367, 414
MluCI AATT 1 cut(s) 202
MnlI CCTC 5 cut(s) 150, 157, 208, 252, 450
MseI TTAA 1 cut(s) 417
MspR9I CCNGG 2 cut(s) 131, 383
MvaI CCWGG 2 cut(s) 131, 383
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 1 cut(s) 418
NdeII GATC 4 cut(s) 3, 34, 217, 460
NlaIII CATG 1 cut(s) 120
NlaIV GGNNCC 2 cut(s) 225, 438
NmuCI GTSAC 1 cut(s) 304
PfeI GAWTC 1 cut(s) 326
PfoI TCCNGGA 1 cut(s) 381
PkrI GCNGC 2 cut(s) 69, 316
PsiI TTATAA 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 414
Psp6I CCWGG 2 cut(s) 129, 381
PspEI GGTNACC 1 cut(s) 304
PspGI CCWGG 2 cut(s) 129, 381
PspN4I GGNNCC 2 cut(s) 225, 438
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 3 cut(s) 49, 225, 233
RsaNI GTAC 3 cut(s) 48, 224, 232
SacI GAGCTC 1 cut(s) 414
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 2 cut(s) 68, 315
Sau3AI GATC 4 cut(s) 3, 34, 217, 460
ScrFI CCNGG 2 cut(s) 131, 383
SduI GDGCHC 2 cut(s) 367, 414
SetI ASST 7 cut(s) 33, 55, 132, 142, 225, 316, 414
SfaNI GCATC 1 cut(s) 344
Sse9I AATT 1 cut(s) 202
SspI AATATT 1 cut(s) 450
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 2 cut(s) 129, 381
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 3 cut(s) 105, 148, 238
TasI AATT 1 cut(s) 202
TatI WGTACW 2 cut(s) 47, 231
TfiI GAWTC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 2 cut(s) 67, 314
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 33, 83, 196
TspRI CASTG 1 cut(s) 243
VneI GTGCAC 1 cut(s) 363
XapI RAATTY 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.