Rroxscaffold_2G00146370
ERF Family

Cupin superfamily (DUF985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013260
Physical Location & Seq
Forward (+)
84267835 .. 84270773
2939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146370.1

Sequence Viewer

Length: 600 bp
ATGGCTCGAGATGACCTTCTCCCGCGAGGGACAATGGAACAGTGGTTGGGGTCGAGGTCAAGGCCCATCCGGTGGACACGCGGGGTTGTAGCGAGACATGCGCGGCCAGAGAAGGAAAGCTTAGACAAAGAGATCGTCCGAACAAGACAAGCAGCAGATCACACAGACATGGCCACTGCCACTGCCACCCAGATTGTTGAGAAACTGAAACTGAATCCACACCCGGAAGGTGGTTTCTTCTCTGAAACTTTCAGAGACACTTCCATTCTCCTCCCCTTGTGTCAGCTCCCACCAGACTACAAGGTTGATCGCCCTGTGAGTACAGCTATTTACTTCTTGCTGCCAACTGGGAATGTTTCGCGCCTTCATCGGATACCGTGTGCAGAAACATGGCATTTCTACCTGGGAGAACCTCTTACGGTACTCTCCAATCATGCTGCTCCAAGGGATTCTGAGACCCACTACTCTCTCGTGGGATGCACTTGTGCACCTGCCTTTCAGTTCCAGGATTTCGAGTTGGCAAAACGCTCAGAGCTCATTAAGCACTTCCCTGATAGCGAGCCTCTTATAACATTGCTAACAGATCCTGAAAAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_5

Protein Analysis

199

Amino Acids

22.58

Weight (kDa)

6.44

Isoelectric Point (pI)

45.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_5 PF06172 64 - 146 4e-23 Cupin superfamily (DUF985)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19130
fragaria_vesca FvH4_1g09020
malus_domestica MD02G1095400.v1.1
prunus_persica Prupe.7G195700_v2.0.a1
pyrus_communis pycom02g07550
rosa_chinensis RchiOBHm_Chr2g0095441
rosa_laevigata RLG00000016566
rosa_multiflora Rmu_co8132630.1_g000001 Rmu_co8397589.1_g000001 Rmu_sc0010847.1_g000003 Rmu_ssc0000183.1_g000046
rosa_roxburghii Rroxscaffold_2G00146370
rosa_rugosa Rorug02G0051100
rosa_samantha Rh2AG099100 Rh2BG100300 Rh2CG102500 Rh2DG100000
rosa_wichuraiana Rw2G007500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
AarI CACCTGC 1 cut(s) 499
Acc36I ACCTGC 1 cut(s) 499
AccB7I CCANNNNNTGG 1 cut(s) 72
AccII CGCG 4 cut(s) 25, 81, 103, 361
AciI CCGC 3 cut(s) 23, 81, 103
AclWI GGATC 1 cut(s) 578
AcoI YGGCCR 2 cut(s) 104, 171
AfaI GTAC 2 cut(s) 322, 423
AfiI CCNNNNNNNGG 2 cut(s) 72, 230
AjnI CCWGG 2 cut(s) 402, 504
AluBI AGCT 4 cut(s) 120, 286, 326, 535
AluI AGCT 4 cut(s) 120, 286, 326, 535
Alw21I GWGCWC 2 cut(s) 490, 537
Alw26I GTCTC 3 cut(s) 88, 249, 449
Alw44I GTGCAC 1 cut(s) 486
AlwI GGATC 1 cut(s) 578
AlwNI CAGNNNCTG 1 cut(s) 587
Ama87I CYCGRG 1 cut(s) 6
AoxI GGCC 3 cut(s) 62, 104, 171
ApaLI GTGCAC 1 cut(s) 486
ApeKI GCWGC 3 cut(s) 152, 340, 437
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AspLEI GCGC 2 cut(s) 103, 363
AspS9I GGNCC 1 cut(s) 63
AsuC2I CCSGG 1 cut(s) 224
AvaI CYCGRG 1 cut(s) 6
BaeGI GKGCMC 1 cut(s) 490
BalI TGGCCA 1 cut(s) 173
BanII GRGCYC 1 cut(s) 537
BauI CACGAG 1 cut(s) 470
Bbv12I GWGCWC 2 cut(s) 490, 537
BbvI GCAGC 3 cut(s) 164, 327, 424
BccI CCATC 1 cut(s) 74
BciT130I CCWGG 2 cut(s) 404, 506
BciVI GTATCC 1 cut(s) 366
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 3 cut(s) 88, 249, 449
BfuAI ACCTGC 1 cut(s) 499
BfuI GTATCC 1 cut(s) 366
BisI GCNGC 4 cut(s) 104, 153, 341, 438
BlsI GCNGC 4 cut(s) 105, 154, 342, 439
Bme1390I CCNGG 3 cut(s) 224, 404, 506
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 63
BmrFI CCNGG 3 cut(s) 224, 404, 506
BmrI ACTGGG 1 cut(s) 357
BmsI GCATC 1 cut(s) 467
BmuI ACTGGG 1 cut(s) 357
BpuMI CCSGG 1 cut(s) 224
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 2 cut(s) 403, 443
BsaWI WCCGGW 1 cut(s) 69
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 230
Bse1I ACTGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 572
BseBI CCWGG 2 cut(s) 404, 506
BseDI CCNNGG 2 cut(s) 403, 443
BseGI GGATG 2 cut(s) 66, 482
BseLI CCNNNNNNNGG 2 cut(s) 72, 230
BseMI GCAATG 1 cut(s) 572
BseMII CTCAG 2 cut(s) 444, 543
BseNI ACTGG 1 cut(s) 352
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 490
BseXI GCAGC 3 cut(s) 164, 327, 424
BsgI GTGCAG 1 cut(s) 402
Bsh1236I CGCG 4 cut(s) 25, 81, 103, 361
BshFI GGCC 3 cut(s) 64, 106, 173
BsiHKAI GWGCWC 2 cut(s) 490, 537
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 2 cut(s) 70, 224
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 2 cut(s) 72, 230
BsmAI GTCTC 3 cut(s) 88, 249, 449
BsmFI GGGAC 1 cut(s) 43
BsnI GGCC 3 cut(s) 64, 106, 173
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 6
Bsp1286I GDGCHC 2 cut(s) 490, 537
Bsp143I GATC 4 cut(s) 132, 157, 307, 583
BspACI CCGC 3 cut(s) 23, 81, 103
BspANI GGCC 3 cut(s) 64, 106, 173
BspCNI CTCAG 2 cut(s) 445, 542
BspFNI CGCG 4 cut(s) 25, 81, 103, 361
BspMI ACCTGC 1 cut(s) 499
BspPI GGATC 1 cut(s) 578
BspTNI GGTCTC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 572
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 403, 443
BssMI GATC 4 cut(s) 132, 157, 307, 583
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 1 cut(s) 443
Bst2BI CACGAG 1 cut(s) 470
Bst2UI CCWGG 2 cut(s) 404, 506
Bst4CI ACNGT 3 cut(s) 42, 378, 421
BstC8I GCNNGC 1 cut(s) 560
BstDEI CTNAG 3 cut(s) 121, 453, 529
BstF5I GGATG 2 cut(s) 66, 482
BstFNI CGCG 4 cut(s) 25, 81, 103, 361
BstHHI GCGC 2 cut(s) 103, 363
BstKTI GATC 4 cut(s) 135, 160, 310, 586
BstMAI GTCTC 3 cut(s) 88, 249, 449
BstMBI GATC 4 cut(s) 132, 157, 307, 583
BstMWI GCNNNNNNNGC 2 cut(s) 98, 541
BstNI CCWGG 2 cut(s) 404, 506
BstNSI RCATGY 1 cut(s) 101
BstSCI CCNGG 3 cut(s) 222, 402, 504
BstSLI GKGCMC 1 cut(s) 490
BstUI CGCG 4 cut(s) 25, 81, 103, 361
BstV1I GCAGC 3 cut(s) 164, 327, 424
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
BsuI GTATCC 1 cut(s) 366
BsuRI GGCC 3 cut(s) 64, 106, 173
BtsCI GGATG 2 cut(s) 66, 482
BtsI GCAGTG 2 cut(s) 174, 180
BtsIMutI CAGTG 3 cut(s) 47, 174, 180
BveI ACCTGC 1 cut(s) 499
Cac8I GCNNGC 1 cut(s) 560
CaiI CAGNNNCTG 1 cut(s) 587
CfoI GCGC 2 cut(s) 103, 363
Cfr13I GGNCC 1 cut(s) 63
Csp6I GTAC 2 cut(s) 321, 422
CspCI CAANNNNNGTGG 2 cut(s) 175, 210
CviAII CATG 4 cut(s) 98, 169, 390, 434
CviJI RGCY 9 cut(s) 5, 64, 106, 120, 173, 286, 326, 535, 562
CviKI_1 RGCY 9 cut(s) 5, 64, 106, 120, 173, 286, 326, 535, 562
CviQI GTAC 2 cut(s) 321, 422
DdeI CTNAG 3 cut(s) 121, 453, 529
DpnI GATC 4 cut(s) 134, 159, 309, 585
DpnII GATC 4 cut(s) 132, 157, 307, 583
EaeI YGGCCR 2 cut(s) 104, 171
Ecl136II GAGCTC 1 cut(s) 535
Eco130I CCWWGG 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 537
Eco31I GGTCTC 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 535
Eco88I CYCGRG 1 cut(s) 6
EcoICRI GAGCTC 1 cut(s) 535
EcoRII CCWGG 2 cut(s) 402, 504
EcoT14I CCWWGG 1 cut(s) 443
EcoT38I GRGCYC 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 443
FaeI CATG 4 cut(s) 101, 172, 393, 437
FaiI YATR 6 cut(s) 99, 170, 391, 435, 569, 598
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FaqI GGGAC 1 cut(s) 43
FatI CATG 4 cut(s) 97, 168, 389, 433
FauI CCCGC 2 cut(s) 30, 74
Fnu4HI GCNGC 4 cut(s) 104, 153, 341, 438
FokI GGATG 2 cut(s) 53, 489
FriOI GRGCYC 1 cut(s) 537
Fsp4HI GCNGC 4 cut(s) 104, 153, 341, 438
GlaI GCGC 2 cut(s) 102, 362
GluI GCNGC 4 cut(s) 104, 153, 341, 438
HaeIII GGCC 3 cut(s) 64, 106, 173
HapII CCGG 2 cut(s) 70, 224
HhaI GCGC 2 cut(s) 103, 363
Hin1II CATG 4 cut(s) 101, 172, 393, 437
Hin6I GCGC 2 cut(s) 101, 361
HinP1I GCGC 2 cut(s) 101, 361
HindIII AAGCTT 1 cut(s) 118
HinfI GANTC 2 cut(s) 214, 449
HpaII CCGG 2 cut(s) 70, 224
Hpy166II GTNNAC 2 cut(s) 75, 488
Hpy188I TCNGA 6 cut(s) 140, 244, 254, 372, 454, 532
Hpy188III TCNNGA 2 cut(s) 8, 587
Hpy8I GTNNAC 2 cut(s) 75, 488
HpyAV CCTTC 4 cut(s) 26, 106, 221, 374
HpyCH4III ACNGT 3 cut(s) 42, 378, 421
HpyCH4V TGCA 3 cut(s) 383, 480, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 541
HpyF3I CTNAG 3 cut(s) 121, 453, 529
Hsp92II CATG 4 cut(s) 101, 172, 393, 437
HspAI GCGC 2 cut(s) 101, 361
Kzo9I GATC 4 cut(s) 132, 157, 307, 583
LmnI GCTCC 2 cut(s) 291, 445
Lsp1109I GCAGC 3 cut(s) 164, 327, 424
LweI GCATC 1 cut(s) 467
MalI GATC 4 cut(s) 134, 159, 309, 585
MboI GATC 4 cut(s) 132, 157, 307, 583
MboII GAAGA 1 cut(s) 229
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 2 cut(s) 490, 537
MlsI TGGCCA 1 cut(s) 173
MluNI TGGCCA 1 cut(s) 173
MnlI CCTC 5 cut(s) 20, 48, 281, 423, 573
Mox20I TGGCCA 1 cut(s) 173
MscI TGGCCA 1 cut(s) 173
MseI TTAA 1 cut(s) 540
MslI CAYNNNNRTG 1 cut(s) 167
Msp20I TGGCCA 1 cut(s) 173
MspI CCGG 2 cut(s) 70, 224
MspR9I CCNGG 3 cut(s) 224, 404, 506
MvaI CCWGG 2 cut(s) 404, 506
MvnI CGCG 4 cut(s) 25, 81, 103, 361
MwoI GCNNNNNNNGC 2 cut(s) 98, 541
NciI CCSGG 1 cut(s) 224
NdeII GATC 4 cut(s) 132, 157, 307, 583
NlaIII CATG 4 cut(s) 101, 172, 393, 437
NspI RCATGY 1 cut(s) 101
PaeR7I CTCGAG 1 cut(s) 6
PaqCI CACCTGC 1 cut(s) 499
PfeI GAWTC 2 cut(s) 214, 449
PflMI CCANNNNNTGG 1 cut(s) 72
PfoI TCCNGGA 1 cut(s) 504
PkrI GCNGC 4 cut(s) 105, 154, 342, 439
PsiI TTATAA 1 cut(s) 569
Psp124BI GAGCTC 1 cut(s) 537
Psp6I CCWGG 2 cut(s) 402, 504
PspGI CCWGG 2 cut(s) 402, 504
PspPI GGNCC 1 cut(s) 63
PstNI CAGNNNCTG 1 cut(s) 587
PsuI RGATCY 1 cut(s) 583
RsaI GTAC 2 cut(s) 322, 423
RsaNI GTAC 2 cut(s) 321, 422
RseI CAYNNNNRTG 1 cut(s) 167
SacI GAGCTC 1 cut(s) 537
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 4 cut(s) 104, 153, 341, 438
Sau3AI GATC 4 cut(s) 132, 157, 307, 583
Sau96I GGNCC 1 cut(s) 63
ScrFI CCNGG 3 cut(s) 224, 404, 506
SduI GDGCHC 2 cut(s) 490, 537
SfaNI GCATC 1 cut(s) 467
Sfr274I CTCGAG 1 cut(s) 6
SlaI CTCGAG 1 cut(s) 6
SmiMI CAYNNNNRTG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SsiI CCGC 3 cut(s) 23, 81, 103
SstI GAGCTC 1 cut(s) 537
StyD4I CCNGG 3 cut(s) 222, 402, 504
StyI CCWWGG 1 cut(s) 443
TaaI ACNGT 3 cut(s) 42, 378, 421
TaqI TCGA 3 cut(s) 7, 53, 513
TatI WGTACW 1 cut(s) 320
TauI GCSGC 1 cut(s) 106
TfiI GAWTC 2 cut(s) 214, 449
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 3 cut(s) 47, 181, 187
TseI GCWGC 3 cut(s) 152, 340, 437
TspDTI ATGAA 1 cut(s) 356
TspRI CASTG 3 cut(s) 47, 181, 187
Van91I CCANNNNNTGG 1 cut(s) 72
VneI GTGCAC 1 cut(s) 486
XceI RCATGY 1 cut(s) 101
XhoI CTCGAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.