Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0007991

Physical Location & Seq

Genomic Coordinates Forward (+)

8248848 .. 8251504

Gene Structure 2657 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1102900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGGAGATATTTGAGGGGACAGTTCCTCGTTCGTCCCTTCCGAAGATAGACAAACACCAACCCCAATTCCGTTATGATGACGAGTATGATGGGTCTGATTGGCCGGAGCCAGAACACTGCGGGAAAGTTAGTTACCCGGCAGTAGCATCCAAGCCCGAGATGCATGACTATTCGTACCAGTTTCCCCTGGAATCCAAAGATTTTGTTGATGGCAGTGGGTATGATTCGAGCGAAGAGCCTTGTGATTTACCTCAGAATAATATGCAACCTGAGGTTAATTTGAAGAATGTGCTGACTGGGGTATTTTCCATTCTGACTGGCCGGAACAAAGCGCCTGGTCTTCCATCAGACAAGCAGCTACCCAATTCGAATGTTTCGTTTTTGGGCTCAGAAAAGAATGGTGATACCTACGTGCACTCGTCGGTTTACATACCCAGTGCCCCACCCCTTCTTGAGCCAACAGCCTTTAATTACAATGCGTACAAAGATGTTTTGGAAGCTGAGCCCCCTGAGTGGCTTCCTGATAGTTCGACTACAGTTTGCATGCAGTGCACTTTGACTTTCACTGCACTTGCTCGTGGGAGGCATCATTGTCGGTTCTGTGGAGGCATTTTCTGCAGAACATGTAGTAAAGGAAGGTGCTTGTTGCCTGTCAAGTTTAGGGAGAGGAATCCTCAGAGGGTCTGTGATGCCTGCTATGATAGGCTAGATCCTTTACAGAGTGTTCTAATTAACAGCATTAGCAATGCTGCGCAAGCGGCAAAGCACGATGTCATGGATTGGACTTGTACAAGAGGATGGTTAAATCTTCCAGTTGGGTTTTCCATGGAACAAGAGATATACAAAGCATCCAACACATTGAGAAGTTATTCCCAGGTTGCTAGGTTGAATCCTGAGAGGTCCATACCTGCAGCAGTTCTCGGAGGAGCCAAAGGTCTGGCAATCTTAACGGTTGCAAAAGCTGGTGCGCTTCTCTCATACAAACTCGGTACTGGTTTGGTAATTGCTCGAAGGTCAGATGGATCATGGTCTGCTCCATCATCCATATTTTCTGTTGGCTTAGGATGGGGTGCACAGATTGGGGGTGAGCTCATGGACTTCATAATCGTGCTTCATGATTTAAAAGCTGTGAAAACATTTTGTAGTCGCATGCATTTTTCTCTTGGTGCTGGGTGTAGTGCTGCAGCAGGACCTATTGGTAGAGTGCTGGAAGCAGACATGCGTGCTGGAGACAGAGGTTCTGGAGTGTGCTATACATACAGTTGTAGCAAAGGTGCATTTGTTGGAGTATCATTGGAAGGGAACATTGTGGCCACTAGGATGGATACCAATCTACAGTTTTATGGTGACCCTTACTTGACGACTTCTGATATACTACTCGGACCTGTGGACAGACCAAAAGCGGCTGAGCCCCTGTATACGGCCCTGAAAAACCTGTACTCCTGTCTACAGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001726 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006996 GO:0007010 GO:0007015 GO:0008092 GO:0008150 GO:0008289 GO:0009987 GO:0015629 GO:0016020 GO:0016043 GO:0016192 GO:0022607 GO:0030029 GO:0030036 GO:0030479 GO:0030863 GO:0030864 GO:0031252 GO:0031253 GO:0031256 GO:0031344 GO:0032587 GO:0035091 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044463 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0051015 GO:0051017 GO:0051128 GO:0051179 GO:0051234 GO:0051641 GO:0051666 GO:0060491 GO:0061572 GO:0061645 GO:0065007 GO:0071840 GO:0071944 GO:0097435 GO:0098590 GO:0098657 GO:0099568 GO:0120025 GO:0120032 GO:0120035 GO:0120038 GO:1900027

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FYVE Ysc84

Protein Analysis

486

Amino Acids

52.95

Weight (kDa)

6.03

Isoelectric Point (pI)

44.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	171 - 236	6e-20	FYVE zinc finger
Ysc84	PF04366	353 - 478	2.6e-38	Las17-binding protein actin regulator

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43230
fragaria_vesca	FvH4_1g09450 FvH4_1g09450
malus_domestica	MD02G1102900.v1.1 MD15G1224000.v1.1
prunus_persica	Prupe.7G191500_v2.0.a1 Prupe.7G191500_v2.0.a1 Prupe.7G191500_v2.0.a1 Prupe.7G191500_v2.0.a1
pyrus_communis	pycom02g08060 pycom15g19820
rosa_chinensis	RchiOBHm_Chr2g0096001 RchiOBHm_Chr2g0096261
rosa_laevigata	RLG00000016616 RLG00000016630
rosa_multiflora	Rmu_co8311399.1_g000001 Rmu_sc0002497.1_g000013
rosa_roxburghii	Rroxscaffold_2G00145870
rosa_rugosa	Rorug02G0055800
rosa_samantha	Rh2AG103700 Rh2BG105200 Rh2CG107700 Rh2DG105600
rosa_wichuraiana	Rw2G007920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	753
Acc36I	ACCTGC	1 cut(s)	916
AccI	GTMKAC	2 cut(s)	1418, 1447
AciI	CCGC	3 cut(s)	120, 758, 1403
AclWI	GGATC	2 cut(s)	704, 1030
AcoI	YGGCCR	3 cut(s)	101, 319, 1311
AfaI	GTAC	5 cut(s)	176, 482, 790, 991, 1439
AfiI	CCNNNNNNNGG	2 cut(s)	513, 1420
AflIII	ACRYGT	1 cut(s)	623
AgsI	TTSAA	2 cut(s)	283, 889
AjnI	CCWGG	3 cut(s)	186, 334, 873
AluBI	AGCT	5 cut(s)	358, 500, 962, 1090, 1127
AluI	AGCT	5 cut(s)	358, 500, 962, 1090, 1127
Alw21I	GWGCWC	4 cut(s)	417, 554, 1075, 1092
Alw26I	GTCTC	1 cut(s)	1224
Alw44I	GTGCAC	3 cut(s)	413, 550, 1071
AlwI	GGATC	2 cut(s)	704, 1030
Ama87I	CYCGRG	1 cut(s)	155
AoxI	GGCC	4 cut(s)	101, 319, 1311, 1422
ApaLI	GTGCAC	3 cut(s)	413, 550, 1071
ApeKI	GCWGC	5 cut(s)	355, 749, 911, 1181, 1184
Asp700I	GAANNNNTTC	1 cut(s)	868
AspLEI	GCGC	3 cut(s)	334, 754, 970
AspS9I	GGNCC	4 cut(s)	900, 1190, 1382, 1423
AsuC2I	CCSGG	1 cut(s)	137
AsuHPI	GGTGA	3 cut(s)	413, 1097, 1358
AsuII	TTCGAA	1 cut(s)	368
AvaI	CYCGRG	1 cut(s)	155
AvaII	GGWCC	3 cut(s)	900, 1190, 1382
AxyI	CCTNAGG	1 cut(s)	270
BaeGI	GKGCMC	4 cut(s)	417, 442, 554, 1075
BaeI	ACNNNNGTAYC	2 cut(s)	396, 429
BalI	TGGCCA	1 cut(s)	1313
BanII	GRGCYC	4 cut(s)	389, 507, 1092, 1413
BauI	CACGAG	1 cut(s)	576
BbsI	GAAGAC	1 cut(s)	332
Bbv12I	GWGCWC	4 cut(s)	417, 554, 1075, 1092
BbvI	GCAGC	5 cut(s)	367, 736, 923, 1168, 1196
BccI	CCATC	8 cut(s)	83, 203, 352, 792, 1013, 1045, 1059, 1315
BceAI	ACGGC	1 cut(s)	1437
BcgI	CGANNNNNNTGC	2 cut(s)	575, 609
BciT130I	CCWGG	3 cut(s)	188, 336, 875
BciVI	GTATCC	1 cut(s)	1318
BcnI	CCSGG	1 cut(s)	137
BcoDI	GTCTC	1 cut(s)	1224
BfaI	CTAG	4 cut(s)	707, 882, 1317, 1456
BfmI	CTRYAG	6 cut(s)	534, 616, 909, 1182, 1334, 1448
BfoI	RGCGCY	1 cut(s)	335
BfuAI	ACCTGC	1 cut(s)	916
BfuI	GTATCC	1 cut(s)	1318
BisI	GCNGC	7 cut(s)	356, 750, 759, 912, 1182, 1185, 1404
BlpI	GCTNAGC	2 cut(s)	501, 1407
BlsI	GCNGC	7 cut(s)	357, 751, 760, 913, 1183, 1186, 1405
Bme1390I	CCNGG	4 cut(s)	137, 188, 336, 875
Bme18I	GGWCC	3 cut(s)	900, 1190, 1382
BmeT110I	CYCGRG	1 cut(s)	155
BmgT120I	GGNCC	4 cut(s)	900, 1190, 1382, 1423
BmiI	GGNNCC	2 cut(s)	108, 928
BmrFI	CCNGG	4 cut(s)	137, 188, 336, 875
BmrI	ACTGGG	2 cut(s)	306, 429
BmsI	GCATC	5 cut(s)	150, 155, 595, 679, 857
BmuI	ACTGGG	2 cut(s)	306, 429
BpiI	GAAGAC	1 cut(s)	332
BplI	GAGNNNNNCTC	2 cut(s)	658, 690
BpmI	CTGGAG	2 cut(s)	1248, 1263
Bpu10I	CCTNAGC	1 cut(s)	1060
Bpu1102I	GCTNAGC	2 cut(s)	501, 1407
Bpu14I	TTCGAA	1 cut(s)	368
BpuEI	CTTGAG	1 cut(s)	473
BpuMI	CCSGG	1 cut(s)	137
BsaAI	YACGTR	1 cut(s)	412
BsaBI	GATNNNNATC	1 cut(s)	1329
BsaJI	CCNNGG	3 cut(s)	186, 825, 873
BsaXI	ACNNNNNCTCC	2 cut(s)	1424, 1454
Bsc4I	CCNNNNNNNGG	2 cut(s)	513, 1420
Bse1I	ACTGG	6 cut(s)	178, 301, 322, 435, 812, 997
Bse21I	CCTNAGG	1 cut(s)	270
Bse3DI	GCAATG	1 cut(s)	751
Bse8I	GATNNNNATC	1 cut(s)	1329
BseBI	CCWGG	3 cut(s)	188, 336, 875
BseDI	CCNNGG	3 cut(s)	186, 825, 873
BseGI	GGATG	6 cut(s)	146, 803, 848, 1040, 1070, 1326
BseJI	GATNNNNATC	1 cut(s)	1329
BseLI	CCNNNNNNNGG	2 cut(s)	513, 1420
BseMI	GCAATG	1 cut(s)	751
BseMII	CTCAG	8 cut(s)	261, 266, 402, 492, 501, 689, 885, 1398
BseNI	ACTGG	6 cut(s)	178, 301, 322, 435, 812, 997
BseRI	GAGGAG	1 cut(s)	939
BseSI	GKGCMC	4 cut(s)	417, 442, 554, 1075
BseXI	GCAGC	5 cut(s)	367, 736, 923, 1168, 1196
BseYI	CCCAGC	1 cut(s)	1169
BsgI	GTGCAG	1 cut(s)	552
BshFI	GGCC	4 cut(s)	103, 321, 1313, 1424
BsiHKAI	GWGCWC	4 cut(s)	417, 554, 1075, 1092
BsiHKCI	CYCGRG	1 cut(s)	155
BsiSI	CCGG	3 cut(s)	104, 137, 322
BslFI	GGGAC	2 cut(s)	19, 31
BslI	CCNNNNNNNGG	2 cut(s)	513, 1420
BsmAI	GTCTC	1 cut(s)	1224
BsmFI	GGGAC	2 cut(s)	19, 31
BsnI	GGCC	4 cut(s)	103, 321, 1313, 1424
BsoBI	CYCGRG	1 cut(s)	155
Bsp119I	TTCGAA	1 cut(s)	368
Bsp1286I	GDGCHC	8 cut(s)	389, 417, 442, 507, 554, 1075, 1092, 1413
Bsp1407I	TGTACA	1 cut(s)	788
Bsp143I	GATC	2 cut(s)	709, 1022
Bsp1720I	GCTNAGC	2 cut(s)	501, 1407
Bsp19I	CCATGG	1 cut(s)	825
BspACI	CCGC	3 cut(s)	120, 758, 1403
BspANI	GGCC	4 cut(s)	103, 321, 1313, 1424
BspCNI	CTCAG	8 cut(s)	262, 265, 401, 493, 502, 688, 886, 1399
BspHI	TCATGA	1 cut(s)	1114
BspLI	GGNNCC	2 cut(s)	108, 928
BspMAI	CTGCAG	3 cut(s)	620, 913, 1186
BspMI	ACCTGC	1 cut(s)	916
BspPI	GGATC	2 cut(s)	704, 1030
BspQI	GCTCTTC	1 cut(s)	228
BspT104I	TTCGAA	1 cut(s)	368
BsrDI	GCAATG	1 cut(s)	751
BsrGI	TGTACA	1 cut(s)	788
BsrI	ACTGG	6 cut(s)	178, 301, 322, 435, 812, 997
BssECI	CCNNGG	3 cut(s)	186, 825, 873
BssMI	GATC	2 cut(s)	709, 1022
BssNAI	GTATAC	1 cut(s)	1419
BssSI	CACGAG	1 cut(s)	576
BssT1I	CCWWGG	1 cut(s)	825
Bst1107I	GTATAC	1 cut(s)	1419
Bst2BI	CACGAG	1 cut(s)	576
Bst2UI	CCWGG	3 cut(s)	188, 336, 875
Bst4CI	ACNGT	6 cut(s)	22, 538, 952, 1262, 1338, 1452
Bst6I	CTCTTC	1 cut(s)	228
BstAPI	GCANNNNNTGC	2 cut(s)	549, 615
BstAUI	TGTACA	1 cut(s)	788
BstBAI	YACGTR	1 cut(s)	412
BstBI	TTCGAA	1 cut(s)	368
BstC8I	GCNNGC	5 cut(s)	545, 694, 756, 1151, 1224
BstDEI	CTNAG	9 cut(s)	252, 270, 388, 501, 510, 675, 894, 1060, 1407
BstDSI	CCRYGG	1 cut(s)	825
BstEII	GGTNACC	1 cut(s)	1346
BstF5I	GGATG	6 cut(s)	146, 803, 848, 1040, 1070, 1326
BstH2I	RGCGCY	1 cut(s)	335
BstHHI	GCGC	3 cut(s)	334, 754, 970
BstKTI	GATC	2 cut(s)	712, 1025
BstMAI	GTCTC	1 cut(s)	1224
BstMBI	GATC	2 cut(s)	709, 1022
BstMWI	GCNNNNNNNGC	5 cut(s)	160, 549, 615, 755, 758
BstNI	CCWGG	3 cut(s)	188, 336, 875
BstNSI	RCATGY	4 cut(s)	547, 627, 1153, 1222
BstPI	GGTNACC	1 cut(s)	1346
BstSCI	CCNGG	4 cut(s)	135, 186, 334, 873
BstSFI	CTRYAG	6 cut(s)	534, 616, 909, 1182, 1334, 1448
BstSLI	GKGCMC	4 cut(s)	417, 442, 554, 1075
BstV1I	GCAGC	5 cut(s)	367, 736, 923, 1168, 1196
BstV2I	GAAGAC	1 cut(s)	332
BstX2I	RGATCY	1 cut(s)	709
BstXI	CCANNNNNNTGG	2 cut(s)	937, 1321
BstYI	RGATCY	1 cut(s)	709
BstZ17I	GTATAC	1 cut(s)	1419
Bsu36I	CCTNAGG	1 cut(s)	270
BsuI	GTATCC	1 cut(s)	1318
BsuRI	GGCC	4 cut(s)	103, 321, 1313, 1424
BtgI	CCRYGG	1 cut(s)	825
BtsCI	GGATG	6 cut(s)	146, 803, 848, 1040, 1070, 1326
BtsI	GCAGTG	4 cut(s)	115, 220, 554, 564
BtsIMutI	CAGTG	6 cut(s)	115, 220, 442, 554, 564, 1457
BveI	ACCTGC	1 cut(s)	916
Cac8I	GCNNGC	5 cut(s)	545, 694, 756, 1151, 1224
CciI	TCATGA	1 cut(s)	1114
CfoI	GCGC	3 cut(s)	334, 754, 970
Cfr13I	GGNCC	4 cut(s)	900, 1190, 1382, 1423
Csp6I	GTAC	5 cut(s)	175, 481, 789, 990, 1438
CviQI	GTAC	5 cut(s)	175, 481, 789, 990, 1438
DdeI	CTNAG	9 cut(s)	252, 270, 388, 501, 510, 675, 894, 1060, 1407
DpnI	GATC	2 cut(s)	711, 1024
DpnII	GATC	2 cut(s)	709, 1022
DraI	TTTAAA	1 cut(s)	1122
EaeI	YGGCCR	3 cut(s)	101, 319, 1311
Eam1104I	CTCTTC	1 cut(s)	228
EarI	CTCTTC	1 cut(s)	228
Ecl136II	GAGCTC	1 cut(s)	1090
Eco130I	CCWWGG	1 cut(s)	825
Eco24I	GRGCYC	4 cut(s)	389, 507, 1092, 1413
Eco47I	GGWCC	3 cut(s)	900, 1190, 1382
Eco53kI	GAGCTC	1 cut(s)	1090
Eco81I	CCTNAGG	1 cut(s)	270
Eco88I	CYCGRG	1 cut(s)	155
Eco91I	GGTNACC	1 cut(s)	1346
EcoICRI	GAGCTC	1 cut(s)	1090
EcoO109I	RGGNCCY	1 cut(s)	1190
EcoO65I	GGTNACC	1 cut(s)	1346
EcoRII	CCWGG	3 cut(s)	186, 334, 873
EcoT14I	CCWWGG	1 cut(s)	825
EcoT22I	ATGCAT	2 cut(s)	165, 1155
EcoT38I	GRGCYC	4 cut(s)	389, 507, 1092, 1413
ErhI	CCWWGG	1 cut(s)	825
FaqI	GGGAC	2 cut(s)	19, 31
FauI	CCCGC	1 cut(s)	113
FblI	GTMKAC	2 cut(s)	1418, 1447
Fnu4HI	GCNGC	7 cut(s)	356, 750, 759, 912, 1182, 1185, 1404
FokI	GGATG	6 cut(s)	133, 810, 835, 1027, 1077, 1333
FriOI	GRGCYC	4 cut(s)	389, 507, 1092, 1413
Fsp4HI	GCNGC	7 cut(s)	356, 750, 759, 912, 1182, 1185, 1404
FspBI	CTAG	4 cut(s)	707, 882, 1317, 1456
FspI	TGCGCA	1 cut(s)	753
GlaI	GCGC	3 cut(s)	333, 753, 969
GluI	GCNGC	7 cut(s)	356, 750, 759, 912, 1182, 1185, 1404
GsaI	CCCAGC	1 cut(s)	1173
GsuI	CTGGAG	2 cut(s)	1248, 1263
HaeII	RGCGCY	1 cut(s)	335
HaeIII	GGCC	4 cut(s)	103, 321, 1313, 1424
HapII	CCGG	3 cut(s)	104, 137, 322
HhaI	GCGC	3 cut(s)	334, 754, 970
Hin6I	GCGC	3 cut(s)	332, 752, 968
HinP1I	GCGC	3 cut(s)	332, 752, 968
HinfI	GANTC	4 cut(s)	191, 224, 670, 889
HpaII	CCGG	3 cut(s)	104, 137, 322
HphI	GGTGA	3 cut(s)	413, 1097, 1358
Hpy166II	GTNNAC	7 cut(s)	415, 427, 552, 1073, 1390, 1419, 1448
Hpy188III	TCNNGA	5 cut(s)	452, 521, 893, 1115, 1242
Hpy8I	GTNNAC	7 cut(s)	415, 427, 552, 1073, 1390, 1419, 1448
Hpy99I	CGWCG	1 cut(s)	424
HpyAV	CCTTC	5 cut(s)	47, 458, 630, 1005, 1292
HpyCH4III	ACNGT	6 cut(s)	22, 538, 952, 1262, 1338, 1452
HpyCH4IV	ACGT	1 cut(s)	411
HpyF10VI	GCNNNNNNNGC	5 cut(s)	160, 549, 615, 755, 758
HpyF3I	CTNAG	9 cut(s)	252, 270, 388, 501, 510, 675, 894, 1060, 1407
HpySE526I	ACGT	1 cut(s)	411
HspAI	GCGC	3 cut(s)	332, 752, 968
Kzo9I	GATC	2 cut(s)	709, 1022
LguI	GCTCTTC	1 cut(s)	228
LmnI	GCTCC	3 cut(s)	106, 926, 1039
Lsp1109I	GCAGC	5 cut(s)	367, 736, 923, 1168, 1196
LweI	GCATC	5 cut(s)	150, 155, 595, 679, 857
MaeI	CTAG	4 cut(s)	707, 882, 1317, 1456
MaeII	ACGT	1 cut(s)	411
MaeIII	GTNAC	2 cut(s)	131, 1346
MalI	GATC	2 cut(s)	711, 1024
MboI	GATC	2 cut(s)	709, 1022
MboII	GAAGA	5 cut(s)	55, 245, 295, 332, 800
MflI	RGATCY	1 cut(s)	709
MhlI	GDGCHC	8 cut(s)	389, 417, 442, 507, 554, 1075, 1092, 1413
MlsI	TGGCCA	1 cut(s)	1313
MluCI	AATT	6 cut(s)	65, 277, 364, 469, 729, 1002
MluNI	TGGCCA	1 cut(s)	1313
MmeI	TCCRAC	2 cut(s)	876, 1264
Mox20I	TGGCCA	1 cut(s)	1313
Mph1103I	ATGCAT	2 cut(s)	165, 1155
MroXI	GAANNNNTTC	1 cut(s)	868
MscI	TGGCCA	1 cut(s)	1313
MseI	TTAA	6 cut(s)	276, 468, 732, 803, 947, 1121
MslI	CAYNNNNRTG	2 cut(s)	1106, 1319
Msp20I	TGGCCA	1 cut(s)	1313
MspI	CCGG	3 cut(s)	104, 137, 322
MspR9I	CCNGG	4 cut(s)	137, 188, 336, 875
MvaI	CCWGG	3 cut(s)	188, 336, 875
MwoI	GCNNNNNNNGC	5 cut(s)	160, 549, 615, 755, 758
NciI	CCSGG	1 cut(s)	137
NcoI	CCATGG	1 cut(s)	825
NdeII	GATC	2 cut(s)	709, 1022
NlaIV	GGNNCC	2 cut(s)	108, 928
NmuCI	GTSAC	1 cut(s)	1346
NsbI	TGCGCA	1 cut(s)	753
NsiI	ATGCAT	2 cut(s)	165, 1155
NspI	RCATGY	4 cut(s)	547, 627, 1153, 1222
NspV	TTCGAA	1 cut(s)	368
PaeI	GCATGC	2 cut(s)	547, 1153
PagI	TCATGA	1 cut(s)	1114
PciI	ACATGT	1 cut(s)	623
PciSI	GCTCTTC	1 cut(s)	228
PcsI	WCGNNNNNNNCGW	2 cut(s)	38, 374
PdmI	GAANNNNTTC	1 cut(s)	868
PfeI	GAWTC	4 cut(s)	191, 224, 670, 889
PkrI	GCNGC	7 cut(s)	357, 751, 760, 913, 1183, 1186, 1405
Ppu21I	YACGTR	1 cut(s)	412
PpuMI	RGGWCCY	1 cut(s)	1190
PscI	ACATGT	1 cut(s)	623
Psp124BI	GAGCTC	1 cut(s)	1092
Psp5II	RGGWCCY	1 cut(s)	1190
Psp6I	CCWGG	3 cut(s)	186, 334, 873
PspEI	GGTNACC	1 cut(s)	1346
PspFI	CCCAGC	1 cut(s)	1169
PspGI	CCWGG	3 cut(s)	186, 334, 873
PspN4I	GGNNCC	2 cut(s)	108, 928
PspPI	GGNCC	4 cut(s)	900, 1190, 1382, 1423
PspPPI	RGGWCCY	1 cut(s)	1190
PstI	CTGCAG	3 cut(s)	620, 913, 1186
PsuI	RGATCY	1 cut(s)	709
RsaI	GTAC	5 cut(s)	176, 482, 790, 991, 1439
RsaNI	GTAC	5 cut(s)	175, 481, 789, 990, 1438
RseI	CAYNNNNRTG	2 cut(s)	1106, 1319
SacI	GAGCTC	1 cut(s)	1092
SapI	GCTCTTC	1 cut(s)	228
SaqAI	TTAA	6 cut(s)	276, 468, 732, 803, 947, 1121
SatI	GCNGC	7 cut(s)	356, 750, 759, 912, 1182, 1185, 1404
Sau3AI	GATC	2 cut(s)	709, 1022
Sau96I	GGNCC	4 cut(s)	900, 1190, 1382, 1423
ScrFI	CCNGG	4 cut(s)	137, 188, 336, 875
SduI	GDGCHC	8 cut(s)	389, 417, 442, 507, 554, 1075, 1092, 1413
SfaNI	GCATC	5 cut(s)	150, 155, 595, 679, 857
SfcI	CTRYAG	6 cut(s)	534, 616, 909, 1182, 1334, 1448
SfuI	TTCGAA	1 cut(s)	368
SinI	GGWCC	3 cut(s)	900, 1190, 1382
SmiMI	CAYNNNNRTG	2 cut(s)	1106, 1319
SmlI	CTYRAG	1 cut(s)	452
SmoI	CTYRAG	1 cut(s)	452
SphI	GCATGC	2 cut(s)	547, 1153
Sse9I	AATT	6 cut(s)	65, 277, 364, 469, 729, 1002
SsiI	CCGC	3 cut(s)	120, 758, 1403
SspMI	CTAG	4 cut(s)	707, 882, 1317, 1456
SstI	GAGCTC	1 cut(s)	1092
StyD4I	CCNGG	4 cut(s)	135, 186, 334, 873
StyI	CCWWGG	1 cut(s)	825
TaaI	ACNGT	6 cut(s)	22, 538, 952, 1262, 1338, 1452
TaiI	ACGT	1 cut(s)	414
TaqI	TCGA	4 cut(s)	227, 368, 530, 1009
TasI	AATT	6 cut(s)	65, 277, 364, 469, 729, 1002
TatI	WGTACW	2 cut(s)	788, 1437
TauI	GCSGC	2 cut(s)	761, 1406
TfiI	GAWTC	4 cut(s)	191, 224, 670, 889
Tru1I	TTAA	6 cut(s)	276, 468, 732, 803, 947, 1121
Tru9I	TTAA	6 cut(s)	276, 468, 732, 803, 947, 1121
TscAI	CASTG	6 cut(s)	122, 220, 442, 554, 571, 1457
TseFI	GTSAC	1 cut(s)	1346
TseI	GCWGC	5 cut(s)	355, 749, 911, 1181, 1184
Tsp45I	GTSAC	1 cut(s)	1346
TspDTI	ATGAA	2 cut(s)	1090, 1103
TspGWI	ACGGA	1 cut(s)	59
TspRI	CASTG	6 cut(s)	122, 220, 442, 554, 571, 1457
VneI	GTGCAC	3 cut(s)	413, 550, 1071
VpaK11BI	GGWCC	3 cut(s)	900, 1190, 1382
XceI	RCATGY	4 cut(s)	547, 627, 1153, 1222
XmiI	GTMKAC	2 cut(s)	1418, 1447
XmnI	GAANNNNTTC	1 cut(s)	868
XspI	CTAG	4 cut(s)	707, 882, 1317, 1456
Zsp2I	ATGCAT	2 cut(s)	165, 1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.