Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007991

Physical Location & Seq

Genomic Coordinates Forward (+)

18219839 .. 18222889

Gene Structure 3051 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1224000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1668 bp

ATGAGGAATTTTTTTCTGCAAAAAAACAGTTGTACCGGATTGGATGTTGTTTTTGTGATCACACACGGACGCCAGGTCTTTTCGTGTATTTCAACAACTCCGGCTGTCTTTTCTTCTCCGTCTTCTTCTTCTTCGTTGGTCGCAAAACCCTGGAAAATTAAATCCCTAATCAAACCAGTTTCTGTCTCAATTTTTGGTTTGAGGAATTGTATGGAGAGCTTTGAGGGGAGAGTTTCGTATTCGTCCCTTCCAAAGATCGACAAACGCCAACCCCAGTTCCACTATGATGACGAGTATGTCGGTTCCGATTGGCCCCAGCCAGAACACTGCGGGAAAGTTAGTTACCCTGCAGTGGCATCCAAGCCCGATAAGCATGATTATTCGTACCAGTTTCCTCTTGAGTCCGAGGATTTTGTTGATGGCAGCGAGTATGATTCGAGTGAAGAGCCTTGTGATTTACCTCAGACTAATACGCAACCAGAAGTCAATTTGAAGAATGTGCTGACTGGGGTATTTGCCATTCTGACTGGCCGGAACAAGGCACCGGGTCTTCCATCAGACAACCAGCTACCTAATTCAAATGTTTCATTTTTGGGATCAGAAAAAAATGGTGATACATGCTTGCACTCGTCGGTTTATACACCCAGTGCCCCACCCCTGCTTGAGCCAACAACTTTTAATTACAACGCATACAAAGATGTTTTGGAAGCTGAGCCCCCTGAATGGCTTCCTGATAGTTCTACTACAGGTTGCATGCAGTGCACTTTGCCTTTTACTGCACTTACTCGTGGAAGACATCATTGTCGGTTCTGTGGAGGAATTTTCTGCAGAACATGTAGTAAAGGAAGGTGCTTGTTACCTGTCAAGTTTAGGGAGAGGAGTCCGCAGAGGGTCTGCGATGCCTGCTATGATAGGCTAGATCCTTTACAGGGTATTCTAATTAACAGCATCAGCAATGCTGCACAAGCAGCCAAGCATGATGTCATCGATTGGACGTGTACAAGAGGATGGTTAAATCTTCCTGTTGGGTTTTCCATGGAACAGGAGATATACAAAGCATCCAACACATTGAGAAGTTACTCCCAGGTTGCTAGGTTGAATCCTGAGAGGTCTATACCCGCAGCTATTCTTAAAGGAGCTAAAGGTCTGGCAATCCTAACAGTTGCTAAAGCTGGTGCACTTCTCTCTTACAAACTTGGTACTGGTTTGGTAATTGCTCGAAGGTCTGATGGATCATGGTCTGCCCCGTCATCCATATTTTCTGTTGGCTTAGGATGGGGCGCGCAGATTGGGGGTGAACTTATGGACTTCATCATTGTGCTTCACGATTTAAAAGCTGTGAAAACATTTTGTAGTCGCATGCATTTTTCTCTTGGTGCTGGGTGTAGTGCTGCCGCAGGACCTATTGGTAGAGTGCTGGAAGCAGACATGCGCGCTGGAGACAGAGGTTCTGGAATGTGCTATACATACAGTTGTAGCAAAGGTGCATTTGTTGGAGTATCATTGGAAGGGAACATTGTGGCCACAAGGATGGATACCAATCTACAGTTTTATGGTGACCCTTACTTGACGACTTCTGATATACTACTCGGAACTGTGGACAGACCAAAGGCTGCTGAGCCCCTGTATACGGCCCTGAAAAACCTCTACTCCAGTCTACAGTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001726 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006996 GO:0007010 GO:0007015 GO:0008092 GO:0008150 GO:0008289 GO:0009987 GO:0015629 GO:0016020 GO:0016043 GO:0016192 GO:0022607 GO:0030029 GO:0030036 GO:0030479 GO:0030863 GO:0030864 GO:0031252 GO:0031253 GO:0031256 GO:0031344 GO:0032587 GO:0035091 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044463 GO:0044464 GO:0044877 GO:0050789 GO:0050794 GO:0051015 GO:0051017 GO:0051128 GO:0051179 GO:0051234 GO:0051641 GO:0051666 GO:0060491 GO:0061572 GO:0061645 GO:0065007 GO:0071840 GO:0071944 GO:0097435 GO:0098590 GO:0098657 GO:0099568 GO:0120025 GO:0120032 GO:0120035 GO:0120038 GO:1900027

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FYVE Ysc84

Protein Analysis

556

Amino Acids

60.57

Weight (kDa)

7.74

Isoelectric Point (pI)

45.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FYVE	PF01363	241 - 306	4.7e-20	FYVE zinc finger
Ysc84	PF04366	423 - 548	1.3e-38	Las17-binding protein actin regulator

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G43230
fragaria_vesca	FvH4_1g09450 FvH4_1g09450
malus_domestica	MD02G1102900.v1.1 MD15G1224000.v1.1
prunus_persica	Prupe.7G191500_v2.0.a1 Prupe.7G191500_v2.0.a1 Prupe.7G191500_v2.0.a1 Prupe.7G191500_v2.0.a1
pyrus_communis	pycom02g08060 pycom15g19820
rosa_chinensis	RchiOBHm_Chr2g0096001 RchiOBHm_Chr2g0096261
rosa_laevigata	RLG00000016616 RLG00000016630
rosa_multiflora	Rmu_co8311399.1_g000001 Rmu_sc0002497.1_g000013
rosa_roxburghii	Rroxscaffold_2G00145870
rosa_rugosa	Rorug02G0055800
rosa_samantha	Rh2AG103700 Rh2BG105200 Rh2CG107700 Rh2DG105600
rosa_wichuraiana	Rw2G007920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	296, 801
AccB1I	GGYRCC	1 cut(s)	541
AccI	GTMKAC	2 cut(s)	1628, 1657
AccII	CGCG	2 cut(s)	1283, 1434
AciI	CCGC	4 cut(s)	330, 884, 1119, 1395
AclWI	GGATC	3 cut(s)	604, 914, 1240
AcoI	YGGCCR	2 cut(s)	529, 1521
AcsI	RAATTY	2 cut(s)	7, 819
AcyI	GRCGYC	1 cut(s)	70
AdeI	CACNNNGTG	1 cut(s)	647
AfaI	GTAC	4 cut(s)	34, 386, 1000, 1201
AfiI	CCNNNNNNNGG	5 cut(s)	352, 538, 723, 929, 1630
AflIII	ACRYGT	2 cut(s)	833, 995
AgsI	TTSAA	4 cut(s)	93, 493, 579, 1099
AhdI	GACNNNNNGTC	1 cut(s)	74
AjiI	CACGTC	1 cut(s)	996
AjnI	CCWGG	3 cut(s)	72, 149, 1083
AluBI	AGCT	7 cut(s)	219, 568, 710, 1124, 1139, 1172, 1337
AluI	AGCT	7 cut(s)	219, 568, 710, 1124, 1139, 1172, 1337
Alw21I	GWGCWC	2 cut(s)	764, 1180
Alw26I	GTCTC	2 cut(s)	190, 1434
Alw44I	GTGCAC	2 cut(s)	760, 1176
AlwI	GGATC	3 cut(s)	604, 914, 1240
AlwNI	CAGNNNCTG	1 cut(s)	182
AoxI	GGCC	4 cut(s)	311, 529, 1521, 1632
ApaLI	GTGCAC	2 cut(s)	760, 1176
ApeKI	GCWGC	6 cut(s)	423, 959, 968, 1121, 1391, 1613
ApoI	RAATTY	2 cut(s)	7, 819
Asp700I	GAANNNNTTC	1 cut(s)	726
AspLEI	GCGC	4 cut(s)	1283, 1285, 1434, 1436
AspS9I	GGNCC	3 cut(s)	312, 1400, 1633
AsuC2I	CCSGG	1 cut(s)	546
AsuHPI	GGTGA	3 cut(s)	623, 1307, 1568
AvaII	GGWCC	1 cut(s)	1400
BaeGI	GKGCMC	3 cut(s)	652, 764, 1180
BaeI	ACNNNNGTAYC	2 cut(s)	16, 49
BalI	TGGCCA	1 cut(s)	1523
BanI	GGYRCC	1 cut(s)	541
BanII	GRGCYC	2 cut(s)	717, 1623
BauI	CACGAG	1 cut(s)	786
BbsI	GAAGAC	3 cut(s)	114, 542, 799
Bbv12I	GWGCWC	2 cut(s)	764, 1180
BbvI	GCAGC	6 cut(s)	435, 946, 980, 1133, 1378, 1600
BccI	CCATC	6 cut(s)	413, 562, 1002, 1223, 1269, 1525
BceAI	ACGGC	1 cut(s)	1647
BciT130I	CCWGG	3 cut(s)	74, 151, 1085
BciVI	GTATCC	1 cut(s)	1528
BclI	TGATCA	1 cut(s)	57
BcnI	CCSGG	1 cut(s)	546
BcoDI	GTCTC	2 cut(s)	190, 1434
BfaI	CTAG	3 cut(s)	917, 1092, 1666
BfmI	CTRYAG	5 cut(s)	348, 744, 826, 1544, 1658
BfuI	GTATCC	1 cut(s)	1528
BisI	GCNGC	7 cut(s)	424, 960, 969, 1122, 1392, 1395, 1614
BlpI	GCTNAGC	2 cut(s)	711, 1617
BlsI	GCNGC	7 cut(s)	425, 961, 970, 1123, 1393, 1396, 1615
Bme1390I	CCNGG	4 cut(s)	74, 151, 546, 1085
Bme18I	GGWCC	1 cut(s)	1400
BmeRI	GACNNNNNGTC	1 cut(s)	74
BmgBI	CACGTC	1 cut(s)	996
BmgT120I	GGNCC	3 cut(s)	312, 1400, 1633
BmiI	GGNNCC	3 cut(s)	304, 314, 543
BmrFI	CCNGG	4 cut(s)	74, 151, 546, 1085
BmrI	ACTGGG	3 cut(s)	268, 516, 639
BmsI	GCATC	4 cut(s)	365, 889, 957, 1067
BmuI	ACTGGG	3 cut(s)	268, 516, 639
BpiI	GAAGAC	3 cut(s)	114, 542, 799
BpmI	CTGGAG	2 cut(s)	1458, 1636
Bpu10I	CCTNAGC	1 cut(s)	1270
Bpu1102I	GCTNAGC	2 cut(s)	711, 1617
BpuEI	CTTGAG	2 cut(s)	419, 683
BpuMI	CCSGG	1 cut(s)	546
Bsa29I	ATCGAT	1 cut(s)	987
BsaBI	GATNNNNATC	1 cut(s)	1539
BsaHI	GRCGYC	1 cut(s)	70
BsaJI	CCNNGG	4 cut(s)	149, 405, 1035, 1083
BsaWI	WCCGGW	1 cut(s)	35
BsaXI	ACNNNNNCTCC	2 cut(s)	1634, 1664
Bsc4I	CCNNNNNNNGG	5 cut(s)	352, 538, 723, 929, 1630
Bse1I	ACTGG	8 cut(s)	176, 274, 388, 511, 532, 645, 1207, 1653
Bse3DI	GCAATG	1 cut(s)	961
Bse8I	GATNNNNATC	1 cut(s)	1539
BseBI	CCWGG	3 cut(s)	74, 151, 1085
BseCI	ATCGAT	1 cut(s)	987
BseDI	CCNNGG	4 cut(s)	149, 405, 1035, 1083
BseGI	GGATG	7 cut(s)	49, 356, 1013, 1058, 1250, 1280, 1536
BseJI	GATNNNNATC	1 cut(s)	1539
BseLI	CCNNNNNNNGG	5 cut(s)	352, 538, 723, 929, 1630
BseMI	GCAATG	1 cut(s)	961
BseMII	CTCAG	4 cut(s)	476, 702, 1095, 1608
BseNI	ACTGG	8 cut(s)	176, 274, 388, 511, 532, 645, 1207, 1653
BsePI	GCGCGC	2 cut(s)	1281, 1432
BseRI	GAGGAG	1 cut(s)	892
BseSI	GKGCMC	3 cut(s)	652, 764, 1180
BseXI	GCAGC	6 cut(s)	435, 946, 980, 1133, 1378, 1600
BseYI	CCCAGC	2 cut(s)	315, 1379
BsgI	GTGCAG	2 cut(s)	762, 945
Bsh1236I	CGCG	2 cut(s)	1283, 1434
BshFI	GGCC	4 cut(s)	313, 531, 1523, 1634
BshNI	GGYRCC	1 cut(s)	541
BshVI	ATCGAT	1 cut(s)	987
BsiHKAI	GWGCWC	2 cut(s)	764, 1180
BsiSI	CCGG	4 cut(s)	36, 101, 532, 545
BslFI	GGGAC	1 cut(s)	229
BslI	CCNNNNNNNGG	5 cut(s)	352, 538, 723, 929, 1630
BsmAI	GTCTC	2 cut(s)	190, 1434
BsmFI	GGGAC	1 cut(s)	229
BsnI	GGCC	4 cut(s)	313, 531, 1523, 1634
Bsp1286I	GDGCHC	5 cut(s)	652, 717, 764, 1180, 1623
Bsp1407I	TGTACA	1 cut(s)	998
Bsp143I	GATC	5 cut(s)	57, 255, 596, 919, 1232
Bsp1720I	GCTNAGC	2 cut(s)	711, 1617
Bsp19I	CCATGG	1 cut(s)	1035
BspACI	CCGC	4 cut(s)	330, 884, 1119, 1395
BspANI	GGCC	4 cut(s)	313, 531, 1523, 1634
BspCNI	CTCAG	4 cut(s)	475, 703, 1096, 1609
BspDI	ATCGAT	1 cut(s)	987
BspFNI	CGCG	2 cut(s)	1283, 1434
BspLI	GGNNCC	3 cut(s)	304, 314, 543
BspMAI	CTGCAG	2 cut(s)	352, 830
BspPI	GGATC	3 cut(s)	604, 914, 1240
BspQI	GCTCTTC	1 cut(s)	438
BspT107I	GGYRCC	1 cut(s)	541
BsrDI	GCAATG	1 cut(s)	961
BsrGI	TGTACA	1 cut(s)	998
BsrI	ACTGG	8 cut(s)	176, 274, 388, 511, 532, 645, 1207, 1653
BssECI	CCNNGG	4 cut(s)	149, 405, 1035, 1083
BssHII	GCGCGC	2 cut(s)	1281, 1432
BssMI	GATC	5 cut(s)	57, 255, 596, 919, 1232
BssNAI	GTATAC	1 cut(s)	1629
BssNI	GRCGYC	1 cut(s)	70
BssSI	CACGAG	1 cut(s)	786
BssT1I	CCWWGG	1 cut(s)	1035
Bst1107I	GTATAC	1 cut(s)	1629
Bst2BI	CACGAG	1 cut(s)	786
Bst2UI	CCWGG	3 cut(s)	74, 151, 1085
Bst4CI	ACNGT	6 cut(s)	29, 1162, 1472, 1548, 1597, 1662
Bst6I	CTCTTC	1 cut(s)	438
BstACI	GRCGYC	1 cut(s)	70
BstAPI	GCANNNNNTGC	1 cut(s)	759
BstAUI	TGTACA	1 cut(s)	998
BstC8I	GCNNGC	6 cut(s)	623, 755, 904, 1283, 1361, 1434
BstDEI	CTNAG	5 cut(s)	462, 711, 1104, 1270, 1617
BstDSI	CCRYGG	1 cut(s)	1035
BstEII	GGTNACC	1 cut(s)	1556
BstF5I	GGATG	7 cut(s)	49, 356, 1013, 1058, 1250, 1280, 1536
BstFNI	CGCG	2 cut(s)	1283, 1434
BstHHI	GCGC	4 cut(s)	1283, 1285, 1434, 1436
BstKTI	GATC	5 cut(s)	60, 258, 599, 922, 1235
BstMAI	GTCTC	2 cut(s)	190, 1434
BstMBI	GATC	5 cut(s)	57, 255, 596, 919, 1232
BstMWI	GCNNNNNNNGC	5 cut(s)	370, 759, 903, 965, 968
BstNI	CCWGG	3 cut(s)	74, 151, 1085
BstNSI	RCATGY	5 cut(s)	621, 757, 837, 1363, 1432
BstPI	GGTNACC	1 cut(s)	1556
BstSCI	CCNGG	4 cut(s)	72, 149, 544, 1083
BstSFI	CTRYAG	5 cut(s)	348, 744, 826, 1544, 1658
BstSLI	GKGCMC	3 cut(s)	652, 764, 1180
BstUI	CGCG	2 cut(s)	1283, 1434
BstV1I	GCAGC	6 cut(s)	435, 946, 980, 1133, 1378, 1600
BstV2I	GAAGAC	3 cut(s)	114, 542, 799
BstX2I	RGATCY	1 cut(s)	919
BstXI	CCANNNNNNTGG	1 cut(s)	1531
BstYI	RGATCY	1 cut(s)	919
BstZ17I	GTATAC	1 cut(s)	1629
Bsu15I	ATCGAT	1 cut(s)	987
BsuI	GTATCC	1 cut(s)	1528
BsuRI	GGCC	4 cut(s)	313, 531, 1523, 1634
BsuTUI	ATCGAT	1 cut(s)	987
BtgI	CCRYGG	1 cut(s)	1035
BtgZI	GCGATG	1 cut(s)	912
BtrI	CACGTC	1 cut(s)	996
BtsCI	GGATG	7 cut(s)	49, 356, 1013, 1058, 1250, 1280, 1536
BtsI	GCAGTG	3 cut(s)	325, 357, 764
BtsIMutI	CAGTG	5 cut(s)	325, 357, 652, 764, 1667
Cac8I	GCNNGC	6 cut(s)	623, 755, 904, 1283, 1361, 1434
CaiI	CAGNNNCTG	1 cut(s)	182
CfoI	GCGC	4 cut(s)	1283, 1285, 1434, 1436
Cfr13I	GGNCC	3 cut(s)	312, 1400, 1633
ClaI	ATCGAT	1 cut(s)	987
CseI	GACGC	1 cut(s)	78
Csp6I	GTAC	4 cut(s)	33, 385, 999, 1200
CviAII	CATG	9 cut(s)	374, 618, 754, 834, 977, 1036, 1236, 1360, 1429
CviQI	GTAC	4 cut(s)	33, 385, 999, 1200
DdeI	CTNAG	5 cut(s)	462, 711, 1104, 1270, 1617
DpnI	GATC	5 cut(s)	59, 257, 598, 921, 1234
DpnII	GATC	5 cut(s)	57, 255, 596, 919, 1232
DraI	TTTAAA	1 cut(s)	1332
DraIII	CACNNNGTG	1 cut(s)	647
DrdI	GACNNNNNNGTC	2 cut(s)	296, 801
DriI	GACNNNNNGTC	1 cut(s)	74
DseDI	GACNNNNNNGTC	2 cut(s)	296, 801
EaeI	YGGCCR	2 cut(s)	529, 1521
Eam1104I	CTCTTC	1 cut(s)	438
Eam1105I	GACNNNNNGTC	1 cut(s)	74
EarI	CTCTTC	1 cut(s)	438
Eco130I	CCWWGG	1 cut(s)	1035
Eco24I	GRGCYC	2 cut(s)	717, 1623
Eco47I	GGWCC	1 cut(s)	1400
Eco91I	GGTNACC	1 cut(s)	1556
EcoO109I	RGGNCCY	1 cut(s)	1400
EcoO65I	GGTNACC	1 cut(s)	1556
EcoRII	CCWGG	3 cut(s)	72, 149, 1083
EcoT14I	CCWWGG	1 cut(s)	1035
EcoT22I	ATGCAT	1 cut(s)	1365
EcoT38I	GRGCYC	2 cut(s)	717, 1623
ErhI	CCWWGG	1 cut(s)	1035
FaeI	CATG	9 cut(s)	377, 621, 757, 837, 980, 1039, 1239, 1363, 1432
FaqI	GGGAC	1 cut(s)	229
FatI	CATG	9 cut(s)	373, 617, 753, 833, 976, 1035, 1235, 1359, 1428
FauI	CCCGC	2 cut(s)	323, 1126
FbaI	TGATCA	1 cut(s)	57
FblI	GTMKAC	2 cut(s)	1628, 1657
Fnu4HI	GCNGC	7 cut(s)	424, 960, 969, 1122, 1392, 1395, 1614
FokI	GGATG	7 cut(s)	56, 343, 1020, 1045, 1237, 1287, 1543
FriOI	GRGCYC	2 cut(s)	717, 1623
Fsp4HI	GCNGC	7 cut(s)	424, 960, 969, 1122, 1392, 1395, 1614
FspBI	CTAG	3 cut(s)	917, 1092, 1666
GlaI	GCGC	4 cut(s)	1282, 1284, 1433, 1435
GluI	GCNGC	7 cut(s)	424, 960, 969, 1122, 1392, 1395, 1614
GsaI	CCCAGC	2 cut(s)	319, 1383
GsuI	CTGGAG	2 cut(s)	1458, 1636
HaeIII	GGCC	4 cut(s)	313, 531, 1523, 1634
HapII	CCGG	4 cut(s)	36, 101, 532, 545
HgaI	GACGC	1 cut(s)	78
HhaI	GCGC	4 cut(s)	1283, 1285, 1434, 1436
Hin1I	GRCGYC	1 cut(s)	70
Hin1II	CATG	9 cut(s)	377, 621, 757, 837, 980, 1039, 1239, 1363, 1432
Hin6I	GCGC	4 cut(s)	1281, 1283, 1432, 1434
HinP1I	GCGC	4 cut(s)	1281, 1283, 1432, 1434
HinfI	GANTC	4 cut(s)	401, 434, 880, 1099
HpaII	CCGG	4 cut(s)	36, 101, 532, 545
HphI	GGTGA	3 cut(s)	623, 1307, 1568
Hpy166II	GTNNAC	7 cut(s)	762, 999, 1178, 1298, 1600, 1629, 1658
Hpy188I	TCNGA	9 cut(s)	307, 406, 465, 525, 559, 601, 1228, 1579, 1592
Hpy188III	TCNNGA	5 cut(s)	398, 731, 1103, 1325, 1452
Hpy8I	GTNNAC	7 cut(s)	762, 999, 1178, 1298, 1600, 1629, 1658
Hpy99I	CGWCG	1 cut(s)	634
HpyAV	CCTTC	4 cut(s)	257, 840, 1215, 1502
HpyCH4III	ACNGT	6 cut(s)	29, 1162, 1472, 1548, 1597, 1662
HpyCH4IV	ACGT	1 cut(s)	995
HpyF10VI	GCNNNNNNNGC	5 cut(s)	370, 759, 903, 965, 968
HpyF3I	CTNAG	5 cut(s)	462, 711, 1104, 1270, 1617
HpySE526I	ACGT	1 cut(s)	995
Hsp92I	GRCGYC	1 cut(s)	70
Hsp92II	CATG	9 cut(s)	377, 621, 757, 837, 980, 1039, 1239, 1363, 1432
HspAI	GCGC	4 cut(s)	1281, 1283, 1432, 1434
Ksp22I	TGATCA	1 cut(s)	57
Kzo9I	GATC	5 cut(s)	57, 255, 596, 919, 1232
LguI	GCTCTTC	1 cut(s)	438
LmnI	GCTCC	1 cut(s)	1136
Lsp1109I	GCAGC	6 cut(s)	435, 946, 980, 1133, 1378, 1600
LweI	GCATC	4 cut(s)	365, 889, 957, 1067
MaeI	CTAG	3 cut(s)	917, 1092, 1666
MaeII	ACGT	1 cut(s)	995
MaeIII	GTNAC	4 cut(s)	341, 855, 1076, 1556
MalI	GATC	5 cut(s)	59, 257, 598, 921, 1234
MboI	GATC	5 cut(s)	57, 255, 596, 919, 1232
MflI	RGATCY	1 cut(s)	919
MhlI	GDGCHC	5 cut(s)	652, 717, 764, 1180, 1623
MlsI	TGGCCA	1 cut(s)	1523
MluNI	TGGCCA	1 cut(s)	1523
MlyI	GAGTC	2 cut(s)	410, 889
MmeI	TCCRAC	2 cut(s)	1086, 1474
Mox20I	TGGCCA	1 cut(s)	1523
Mph1103I	ATGCAT	1 cut(s)	1365
MroXI	GAANNNNTTC	1 cut(s)	726
MscI	TGGCCA	1 cut(s)	1523
MseI	TTAA	6 cut(s)	159, 678, 942, 1013, 1131, 1331
MslI	CAYNNNNRTG	3 cut(s)	285, 1316, 1529
Msp20I	TGGCCA	1 cut(s)	1523
MspI	CCGG	4 cut(s)	36, 101, 532, 545
MspR9I	CCNGG	4 cut(s)	74, 151, 546, 1085
MvaI	CCWGG	3 cut(s)	74, 151, 1085
MvnI	CGCG	2 cut(s)	1283, 1434
MwoI	GCNNNNNNNGC	5 cut(s)	370, 759, 903, 965, 968
NciI	CCSGG	1 cut(s)	546
NcoI	CCATGG	1 cut(s)	1035
NdeII	GATC	5 cut(s)	57, 255, 596, 919, 1232
NlaIII	CATG	9 cut(s)	377, 621, 757, 837, 980, 1039, 1239, 1363, 1432
NlaIV	GGNNCC	3 cut(s)	304, 314, 543
NmuCI	GTSAC	1 cut(s)	1556
NsiI	ATGCAT	1 cut(s)	1365
NspI	RCATGY	5 cut(s)	621, 757, 837, 1363, 1432
PaeI	GCATGC	2 cut(s)	757, 1363
PauI	GCGCGC	2 cut(s)	1281, 1432
PciI	ACATGT	1 cut(s)	833
PciSI	GCTCTTC	1 cut(s)	438
PdmI	GAANNNNTTC	1 cut(s)	726
PfeI	GAWTC	2 cut(s)	434, 1099
PkrI	GCNGC	7 cut(s)	425, 961, 970, 1123, 1393, 1396, 1615
PleI	GAGTC	2 cut(s)	409, 888
PpsI	GAGTC	2 cut(s)	409, 888
PpuMI	RGGWCCY	1 cut(s)	1400
PscI	ACATGT	1 cut(s)	833
Psp5II	RGGWCCY	1 cut(s)	1400
Psp6I	CCWGG	3 cut(s)	72, 149, 1083
PspEI	GGTNACC	1 cut(s)	1556
PspFI	CCCAGC	2 cut(s)	315, 1379
PspGI	CCWGG	3 cut(s)	72, 149, 1083
PspN4I	GGNNCC	3 cut(s)	304, 314, 543
PspPI	GGNCC	3 cut(s)	312, 1400, 1633
PspPPI	RGGWCCY	1 cut(s)	1400
PstI	CTGCAG	2 cut(s)	352, 830
PstNI	CAGNNNCTG	1 cut(s)	182
PsuI	RGATCY	1 cut(s)	919
PteI	GCGCGC	2 cut(s)	1281, 1432
RsaI	GTAC	4 cut(s)	34, 386, 1000, 1201
RsaNI	GTAC	4 cut(s)	33, 385, 999, 1200
RseI	CAYNNNNRTG	3 cut(s)	285, 1316, 1529
SapI	GCTCTTC	1 cut(s)	438
SaqAI	TTAA	6 cut(s)	159, 678, 942, 1013, 1131, 1331
SatI	GCNGC	7 cut(s)	424, 960, 969, 1122, 1392, 1395, 1614
Sau3AI	GATC	5 cut(s)	57, 255, 596, 919, 1232
Sau96I	GGNCC	3 cut(s)	312, 1400, 1633
SchI	GAGTC	2 cut(s)	410, 889
ScrFI	CCNGG	4 cut(s)	74, 151, 546, 1085
SduI	GDGCHC	5 cut(s)	652, 717, 764, 1180, 1623
SfaNI	GCATC	4 cut(s)	365, 889, 957, 1067
SfcI	CTRYAG	5 cut(s)	348, 744, 826, 1544, 1658
SinI	GGWCC	1 cut(s)	1400
SmiMI	CAYNNNNRTG	3 cut(s)	285, 1316, 1529
SmlI	CTYRAG	2 cut(s)	398, 662
SmoI	CTYRAG	2 cut(s)	398, 662
SphI	GCATGC	2 cut(s)	757, 1363
SsiI	CCGC	4 cut(s)	330, 884, 1119, 1395
SspMI	CTAG	3 cut(s)	917, 1092, 1666
StyD4I	CCNGG	4 cut(s)	72, 149, 544, 1083
StyI	CCWWGG	1 cut(s)	1035
TaaI	ACNGT	6 cut(s)	29, 1162, 1472, 1548, 1597, 1662
TaiI	ACGT	1 cut(s)	998
TaqI	TCGA	4 cut(s)	258, 437, 987, 1219
TatI	WGTACW	1 cut(s)	998
TauI	GCSGC	1 cut(s)	1397
TfiI	GAWTC	2 cut(s)	434, 1099
Tru1I	TTAA	6 cut(s)	159, 678, 942, 1013, 1131, 1331
Tru9I	TTAA	6 cut(s)	159, 678, 942, 1013, 1131, 1331
TscAI	CASTG	5 cut(s)	332, 357, 652, 764, 1667
TseFI	GTSAC	1 cut(s)	1556
TseI	GCWGC	6 cut(s)	423, 959, 968, 1121, 1391, 1613
Tsp45I	GTSAC	1 cut(s)	1556
TspDTI	ATGAA	2 cut(s)	576, 1300
TspGWI	ACGGA	2 cut(s)	81, 108
TspRI	CASTG	5 cut(s)	332, 357, 652, 764, 1667
VneI	GTGCAC	2 cut(s)	760, 1176
VpaK11BI	GGWCC	1 cut(s)	1400
XapI	RAATTY	2 cut(s)	7, 819
XceI	RCATGY	5 cut(s)	621, 757, 837, 1363, 1432
XmiI	GTMKAC	2 cut(s)	1628, 1657
XmnI	GAANNNNTTC	1 cut(s)	726
XspI	CTAG	3 cut(s)	917, 1092, 1666
Zsp2I	ATGCAT	1 cut(s)	1365

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.