Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001777

Physical Location & Seq

Genomic Coordinates Reverse (-)

11709930 .. 11718821

Gene Structure 8892 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1140800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1143 bp

ATGGACCGCTATCTCAAGTACCATGTGCAGGAGTTTGAAAGGACATTTGCGGTTAAGTTTCCAGCTTGTTCAATTGCAGCAAAGAAGAACATTGATCAAAGTACAACTATCCTCGATGTGCAAGGAGTGGGATTTAAGAATTTCAACAAAGCTGCCAGGGAACTTATTACTCGCCTTCAGAAGATTGACGGTGACAATTATCCTGAGACCTTGAATCGCATGTTTATCATCAACGCTGGTTCTGGCTTTAGAATGTTGTGGAACAGCGTGAAGTCGTTCCTTGACCCGAAGACTACAGCAAAGATTAATGTCCTTGGCAACAAGTATCAGAGCAAGTTGCTTGAAATAATTGATGCTAGCGAATTGCCCAAGTTTTTAGGAGGTACATGTACTTGTTCTGATCAAGGAGGCTGTATGCGTTCGGACAAGGGACCCTGGAAACAACCTGAGATCGTGAAGATGGTTCAAAACGGCATTCATAAATGTTCAAGAAAACCTGGAGTTCAAGTATCGCTAGCCAAAACTCGCGAAGCGTCTTTCAGGCGCGAGGACAGTGCCTCGATGGTTGGTAAATCCGTGGATTCAAGTTGGCCAATGGCGACGGGGAATGTTAAATTTGCCCTCTCAAACGCTAAATCATCTAAGAATTTAGCAGGAGATTGTTTCACCAGGAACAATGCTTGCAAACTTCCTGAAGGGTTCGGCTCTCAGATTATGGCTGCTATTCTGACGTTTCTTATGGGCATTGTCGCCATGGTCAGACTAACACGTAGCATGCCGAAGAGGCTCACTGACTCCACCTTCTACTCCAGCACTGTCTGCGACGGTGACACAATGGTCAAAGGCCAGGGCTCTTCACACGCTGCCATCTCCGGCTCAGATCTCATGACTGTCATGAAACGCATGGCTGAGTTGGAAGAGAGAATGACTGTTTTGAACATGAAACCAGCCACCGTGCCAGCTCAGAAGGAGGAAATGCTGAATACTGCTCTAGGCCGGGTTGGTGCCTTGGAGAAAGAGCTTATGGCAACAAAGCAGGCTCTCGAGGAGTCGCTTACCCAGCAAGAGGAACTCTTTGGCTCTCTTGACAAAAAGAAGAAGAGGAAGAACAACATGAAGAAGAAAATGTTCAATATCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0003006 GO:0003674 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005628 GO:0005634 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0008150 GO:0008525 GO:0008526 GO:0009653 GO:0009987 GO:0010876 GO:0010927 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016192 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022607 GO:0030154 GO:0030427 GO:0030435 GO:0030437 GO:0031321 GO:0031322 GO:0032153 GO:0032502 GO:0032505 GO:0032989 GO:0033036 GO:0034293 GO:0035838 GO:0042763 GO:0042764 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043934 GO:0043935 GO:0044085 GO:0044424 GO:0044464 GO:0044703 GO:0048193 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051286 GO:0051321 GO:0051704 GO:0060187 GO:0061024 GO:0071702 GO:0071840 GO:0120009 GO:0120010 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

381

Amino Acids

42.26

Weight (kDa)

9.6

Isoelectric Point (pI)

47.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO	PF00650	6 - 128	3.3e-23	CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001777)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18180 AT2G18180 AT2G18180 AT2G18180 AT2G18180 AT4G36490
fragaria_vesca	FvH4_1g03070 FvH4_1g12640
malus_domestica	MD02G1140800.v1.1 MD15G1253600.v1.1
prunus_persica	Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G244900_v2.0.a1
pyrus_communis	pycom02g11090 pycom15g22320
rosa_chinensis	RchiOBHm_Chr2g0088201 RchiOBHm_Chr2g0100591 RchiOBHm_Chr2g0100601
rosa_laevigata	RLG00000015931
rosa_multiflora	Rmu_sc0001497.1_g000001 Rmu_sc0003275.1_g000070
rosa_roxburghii	Rroxscaffold_2G00142100 Rroxscaffold_7G00200090
rosa_rugosa	Rorug01G0481700 Rorug02G0089800 Rorug02G0089900 Rorug02G0090000 Rorug02G0090100 Rorug06G0039000 Rorug06G0389100
rosa_samantha	Rh2AG036800 Rh2AG139000 Rh2BG036000 Rh2BG142300 Rh2CG037100 Rh2CG144600 Rh2DG036500 Rh2DG144000
rosa_wichuraiana	Rw2G002900 Rw2G010890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	836
AccB1I	GGYRCC	1 cut(s)	1004
AccII	CGCG	2 cut(s)	528, 546
AciI	CCGC	2 cut(s)	7, 50
AcoI	YGGCCR	1 cut(s)	590
AcsI	RAATTY	3 cut(s)	139, 614, 646
AcuI	CTGAAG	2 cut(s)	161, 714
AfaI	GTAC	4 cut(s)	20, 103, 385, 391
AfiI	CCNNNNNNNGG	2 cut(s)	28, 1066
AflIII	ACRYGT	2 cut(s)	386, 767
AjnI	CCWGG	5 cut(s)	155, 434, 496, 668, 846
AluBI	AGCT	4 cut(s)	65, 152, 962, 1021
AluI	AGCT	4 cut(s)	65, 152, 962, 1021
Alw26I	GTCTC	1 cut(s)	200
Ama87I	CYCGRG	1 cut(s)	1043
AoxI	GGCC	3 cut(s)	590, 844, 994
ApeKI	GCWGC	4 cut(s)	77, 152, 719, 863
ApoI	RAATTY	3 cut(s)	139, 614, 646
AseI	ATTAAT	1 cut(s)	306
Asp700I	GAANNNNTTC	2 cut(s)	275, 1127
AspLEI	GCGC	1 cut(s)	546
AspS9I	GGNCC	2 cut(s)	4, 431
AsuC2I	CCSGG	1 cut(s)	998
AsuHPI	GGTGA	3 cut(s)	203, 658, 839
AsuNHI	GCTAGC	2 cut(s)	356, 514
AvaI	CYCGRG	1 cut(s)	1043
AvaII	GGWCC	2 cut(s)	4, 431
BalI	TGGCCA	1 cut(s)	592
BanI	GGYRCC	1 cut(s)	1004
BanII	GRGCYC	1 cut(s)	854
BbsI	GAAGAC	1 cut(s)	296
BbvI	GCAGC	4 cut(s)	89, 139, 706, 850
BccI	CCATC	3 cut(s)	454, 556, 875
BceAI	ACGGC	1 cut(s)	487
BcgI	CGANNNNNNTGC	2 cut(s)	536, 570
BciT130I	CCWGG	5 cut(s)	157, 436, 498, 670, 848
BclI	TGATCA	2 cut(s)	94, 400
BcnI	CCSGG	1 cut(s)	998
BcoDI	GTCTC	1 cut(s)	200
BfaI	CTAG	3 cut(s)	357, 515, 992
BfmI	CTRYAG	1 cut(s)	294
BglI	GCCNNNNNGGC	1 cut(s)	784
BglII	AGATCT	1 cut(s)	880
BisI	GCNGC	4 cut(s)	78, 153, 720, 864
BlsI	GCNGC	4 cut(s)	79, 154, 721, 865
Bme1390I	CCNGG	6 cut(s)	157, 436, 498, 670, 848, 998
Bme18I	GGWCC	2 cut(s)	4, 431
BmeT110I	CYCGRG	1 cut(s)	1043
BmgT120I	GGNCC	2 cut(s)	4, 431
BmiI	GGNNCC	3 cut(s)	432, 433, 1006
BmrFI	CCNGG	6 cut(s)	157, 436, 498, 670, 848, 998
BmsI	GCATC	1 cut(s)	343
BmtI	GCTAGC	2 cut(s)	360, 518
BpiI	GAAGAC	1 cut(s)	296
BpmI	CTGGAG	2 cut(s)	519, 793
BpuMI	CCSGG	1 cut(s)	998
BsaAI	YACGTR	1 cut(s)	770
BsaI	GGTCTC	1 cut(s)	200
BsaJI	CCNNGG	7 cut(s)	156, 313, 434, 576, 753, 847, 1008
BsaXI	ACNNNNNCTCC	2 cut(s)	492, 522
Bsc4I	CCNNNNNNNGG	2 cut(s)	28, 1066
BseBI	CCWGG	5 cut(s)	157, 436, 498, 670, 848
BseDI	CCNNGG	7 cut(s)	156, 313, 434, 576, 753, 847, 1008
BseLI	CCNNNNNNNGG	2 cut(s)	28, 1066
BseMII	CTCAG	6 cut(s)	195, 438, 722, 891, 900, 977
BseRI	GAGGAG	1 cut(s)	1061
BseXI	GCAGC	4 cut(s)	89, 139, 706, 850
BseYI	CCCAGC	1 cut(s)	1059
BsgI	GTGCAG	1 cut(s)	47
Bsh1236I	CGCG	2 cut(s)	528, 546
BshFI	GGCC	3 cut(s)	592, 846, 996
BshNI	GGYRCC	1 cut(s)	1004
BsiHKCI	CYCGRG	1 cut(s)	1043
BsiSI	CCGG	2 cut(s)	873, 997
BslFI	GGGAC	1 cut(s)	444
BslI	CCNNNNNNNGG	2 cut(s)	28, 1066
BsmAI	GTCTC	1 cut(s)	200
BsmFI	GGGAC	1 cut(s)	444
BsmI	GAATGC	1 cut(s)	474
BsnI	GGCC	3 cut(s)	592, 846, 996
Bso31I	GGTCTC	1 cut(s)	200
BsoBI	CYCGRG	1 cut(s)	1043
Bsp1286I	GDGCHC	1 cut(s)	854
Bsp143I	GATC	4 cut(s)	94, 400, 450, 880
Bsp19I	CCATGG	1 cut(s)	753
Bsp68I	TCGCGA	1 cut(s)	528
BspACI	CCGC	2 cut(s)	7, 50
BspANI	GGCC	3 cut(s)	592, 846, 996
BspCNI	CTCAG	6 cut(s)	196, 439, 721, 890, 901, 976
BspFNI	CGCG	2 cut(s)	528, 546
BspHI	TCATGA	2 cut(s)	885, 894
BspLI	GGNNCC	3 cut(s)	432, 433, 1006
BspOI	GCTAGC	2 cut(s)	360, 518
BspQI	GCTCTTC	1 cut(s)	859
BspT107I	GGYRCC	1 cut(s)	1004
BspTNI	GGTCTC	1 cut(s)	200
BssECI	CCNNGG	7 cut(s)	156, 313, 434, 576, 753, 847, 1008
BssMI	GATC	4 cut(s)	94, 400, 450, 880
BssT1I	CCWWGG	3 cut(s)	313, 753, 1008
Bst2UI	CCWGG	5 cut(s)	157, 436, 498, 670, 848
Bst4CI	ACNGT	7 cut(s)	191, 554, 817, 827, 892, 931, 955
Bst6I	CTCTTC	4 cut(s)	776, 859, 912, 1094
BstAPI	GCANNNNNTGC	1 cut(s)	819
BstBAI	YACGTR	1 cut(s)	770
BstC8I	GCNNGC	6 cut(s)	358, 516, 682, 776, 960, 1038
BstDEI	CTNAG	7 cut(s)	204, 447, 642, 708, 877, 909, 963
BstDSI	CCRYGG	2 cut(s)	576, 753
BstFNI	CGCG	2 cut(s)	528, 546
BstHHI	GCGC	1 cut(s)	546
BstKTI	GATC	4 cut(s)	97, 403, 453, 883
BstMAI	GTCTC	1 cut(s)	200
BstMBI	GATC	4 cut(s)	94, 400, 450, 880
BstMWI	GCNNNNNNNGC	3 cut(s)	784, 819, 1060
BstNI	CCWGG	5 cut(s)	157, 436, 498, 670, 848
BstNSI	RCATGY	3 cut(s)	223, 390, 778
BstSCI	CCNGG	6 cut(s)	155, 434, 496, 668, 846, 996
BstSFI	CTRYAG	1 cut(s)	294
BstUI	CGCG	2 cut(s)	528, 546
BstV1I	GCAGC	4 cut(s)	89, 139, 706, 850
BstV2I	GAAGAC	1 cut(s)	296
BstX2I	RGATCY	1 cut(s)	880
BstYI	RGATCY	1 cut(s)	880
BsuRI	GGCC	3 cut(s)	592, 846, 996
BtgI	CCRYGG	2 cut(s)	576, 753
BtsIMutI	CAGTG	3 cut(s)	559, 789, 813
BtuMI	TCGCGA	1 cut(s)	528
Cac8I	GCNNGC	6 cut(s)	358, 516, 682, 776, 960, 1038
CciI	TCATGA	2 cut(s)	885, 894
CfoI	GCGC	1 cut(s)	546
Cfr13I	GGNCC	2 cut(s)	4, 431
CseI	GACGC	1 cut(s)	522
Csp6I	GTAC	4 cut(s)	19, 102, 384, 390
CviQI	GTAC	4 cut(s)	19, 102, 384, 390
DdeI	CTNAG	7 cut(s)	204, 447, 642, 708, 877, 909, 963
DpnI	GATC	4 cut(s)	96, 402, 452, 882
DpnII	GATC	4 cut(s)	94, 400, 450, 880
DrdI	GACNNNNNNGTC	1 cut(s)	836
DseDI	GACNNNNNNGTC	1 cut(s)	836
EaeI	YGGCCR	1 cut(s)	590
Eam1104I	CTCTTC	4 cut(s)	776, 859, 912, 1094
EarI	CTCTTC	4 cut(s)	776, 859, 912, 1094
Eco130I	CCWWGG	3 cut(s)	313, 753, 1008
Eco24I	GRGCYC	1 cut(s)	854
Eco31I	GGTCTC	1 cut(s)	200
Eco47I	GGWCC	2 cut(s)	4, 431
Eco57I	CTGAAG	2 cut(s)	161, 714
Eco88I	CYCGRG	1 cut(s)	1043
EcoO109I	RGGNCCY	1 cut(s)	431
EcoRII	CCWGG	5 cut(s)	155, 434, 496, 668, 846
EcoT14I	CCWWGG	3 cut(s)	313, 753, 1008
EcoT38I	GRGCYC	1 cut(s)	854
ErhI	CCWWGG	3 cut(s)	313, 753, 1008
FaqI	GGGAC	1 cut(s)	444
FbaI	TGATCA	2 cut(s)	94, 400
Fnu4HI	GCNGC	4 cut(s)	78, 153, 720, 864
FriOI	GRGCYC	1 cut(s)	854
Fsp4HI	GCNGC	4 cut(s)	78, 153, 720, 864
FspBI	CTAG	3 cut(s)	357, 515, 992
GlaI	GCGC	1 cut(s)	545
GluI	GCNGC	4 cut(s)	78, 153, 720, 864
GsaI	CCCAGC	1 cut(s)	1063
GsuI	CTGGAG	2 cut(s)	519, 793
HaeIII	GGCC	3 cut(s)	592, 846, 996
HapII	CCGG	2 cut(s)	873, 997
HgaI	GACGC	1 cut(s)	522
HhaI	GCGC	1 cut(s)	546
Hin6I	GCGC	1 cut(s)	544
HinP1I	GCGC	1 cut(s)	544
HinfI	GANTC	4 cut(s)	214, 581, 794, 1049
HpaII	CCGG	2 cut(s)	873, 997
HphI	GGTGA	3 cut(s)	203, 658, 839
Hpy188I	TCNGA	9 cut(s)	180, 330, 400, 424, 711, 729, 761, 880, 966
Hpy188III	TCNNGA	9 cut(s)	203, 454, 489, 527, 692, 886, 895, 1043, 1085
Hpy99I	CGWCG	2 cut(s)	604, 827
HpyAV	CCTTC	4 cut(s)	185, 689, 811, 961
HpyCH4III	ACNGT	7 cut(s)	191, 554, 817, 827, 892, 931, 955
HpyCH4IV	ACGT	2 cut(s)	731, 769
HpyCH4V	TGCA	4 cut(s)	28, 77, 121, 684
HpyF10VI	GCNNNNNNNGC	3 cut(s)	784, 819, 1060
HpyF3I	CTNAG	7 cut(s)	204, 447, 642, 708, 877, 909, 963
HpySE526I	ACGT	2 cut(s)	731, 769
HspAI	GCGC	1 cut(s)	544
KflI	GGGWCCC	1 cut(s)	431
Ksp22I	TGATCA	2 cut(s)	94, 400
Kzo9I	GATC	4 cut(s)	94, 400, 450, 880
LguI	GCTCTTC	1 cut(s)	859
Lsp1109I	GCAGC	4 cut(s)	89, 139, 706, 850
LweI	GCATC	1 cut(s)	343
MaeI	CTAG	3 cut(s)	357, 515, 992
MaeII	ACGT	2 cut(s)	731, 769
MaeIII	GTNAC	2 cut(s)	191, 827
MalI	GATC	4 cut(s)	96, 402, 452, 882
MboI	GATC	4 cut(s)	94, 400, 450, 880
MfeI	CAATTG	1 cut(s)	72
MflI	RGATCY	1 cut(s)	880
MhlI	GDGCHC	1 cut(s)	854
MlsI	TGGCCA	1 cut(s)	592
MluCI	AATT	7 cut(s)	72, 139, 196, 348, 362, 614, 646
MluNI	TGGCCA	1 cut(s)	592
MlyI	GAGTC	2 cut(s)	788, 1058
MmeI	TCCRAC	1 cut(s)	894
Mox20I	TGGCCA	1 cut(s)	592
MroXI	GAANNNNTTC	2 cut(s)	275, 1127
MscI	TGGCCA	1 cut(s)	592
MseI	TTAA	4 cut(s)	54, 135, 306, 612
Msp20I	TGGCCA	1 cut(s)	592
MspI	CCGG	2 cut(s)	873, 997
MspR9I	CCNGG	6 cut(s)	157, 436, 498, 670, 848, 998
MunI	CAATTG	1 cut(s)	72
Mva1269I	GAATGC	1 cut(s)	474
MvaI	CCWGG	5 cut(s)	157, 436, 498, 670, 848
MvnI	CGCG	2 cut(s)	528, 546
MwoI	GCNNNNNNNGC	3 cut(s)	784, 819, 1060
NciI	CCSGG	1 cut(s)	998
NcoI	CCATGG	1 cut(s)	753
NdeII	GATC	4 cut(s)	94, 400, 450, 880
NheI	GCTAGC	2 cut(s)	356, 514
NlaIV	GGNNCC	3 cut(s)	432, 433, 1006
NmuCI	GTSAC	2 cut(s)	191, 827
NruI	TCGCGA	1 cut(s)	528
NspI	RCATGY	3 cut(s)	223, 390, 778
PaeI	GCATGC	1 cut(s)	778
PaeR7I	CTCGAG	1 cut(s)	1043
PagI	TCATGA	2 cut(s)	885, 894
PciI	ACATGT	1 cut(s)	386
PciSI	GCTCTTC	1 cut(s)	859
PctI	GAATGC	1 cut(s)	474
PdmI	GAANNNNTTC	2 cut(s)	275, 1127
PfeI	GAWTC	2 cut(s)	214, 581
PkrI	GCNGC	4 cut(s)	79, 154, 721, 865
PleI	GAGTC	2 cut(s)	788, 1057
PpsI	GAGTC	2 cut(s)	788, 1057
Ppu21I	YACGTR	1 cut(s)	770
PpuMI	RGGWCCY	1 cut(s)	431
PscI	ACATGT	1 cut(s)	386
PshBI	ATTAAT	1 cut(s)	306
Psp5II	RGGWCCY	1 cut(s)	431
Psp6I	CCWGG	5 cut(s)	155, 434, 496, 668, 846
PspFI	CCCAGC	1 cut(s)	1059
PspGI	CCWGG	5 cut(s)	155, 434, 496, 668, 846
PspN4I	GGNNCC	3 cut(s)	432, 433, 1006
PspPI	GGNCC	2 cut(s)	4, 431
PspPPI	RGGWCCY	1 cut(s)	431
PsuI	RGATCY	1 cut(s)	880
RruI	TCGCGA	1 cut(s)	528
RsaI	GTAC	4 cut(s)	20, 103, 385, 391
RsaNI	GTAC	4 cut(s)	19, 102, 384, 390
SapI	GCTCTTC	1 cut(s)	859
SaqAI	TTAA	4 cut(s)	54, 135, 306, 612
SatI	GCNGC	4 cut(s)	78, 153, 720, 864
Sau3AI	GATC	4 cut(s)	94, 400, 450, 880
Sau96I	GGNCC	2 cut(s)	4, 431
SchI	GAGTC	2 cut(s)	788, 1058
ScrFI	CCNGG	6 cut(s)	157, 436, 498, 670, 848, 998
SduI	GDGCHC	1 cut(s)	854
SfaNI	GCATC	1 cut(s)	343
SfcI	CTRYAG	1 cut(s)	294
Sfr274I	CTCGAG	1 cut(s)	1043
SinI	GGWCC	2 cut(s)	4, 431
SlaI	CTCGAG	1 cut(s)	1043
SmlI	CTYRAG	2 cut(s)	14, 1043
SmoI	CTYRAG	2 cut(s)	14, 1043
SphI	GCATGC	1 cut(s)	778
Sse9I	AATT	7 cut(s)	72, 139, 196, 348, 362, 614, 646
SsiI	CCGC	2 cut(s)	7, 50
SspMI	CTAG	3 cut(s)	357, 515, 992
StyD4I	CCNGG	6 cut(s)	155, 434, 496, 668, 846, 996
StyI	CCWWGG	3 cut(s)	313, 753, 1008
TaaI	ACNGT	7 cut(s)	191, 554, 817, 827, 892, 931, 955
TaiI	ACGT	2 cut(s)	734, 772
TaqI	TCGA	3 cut(s)	114, 560, 1044
TasI	AATT	7 cut(s)	72, 139, 196, 348, 362, 614, 646
TatI	WGTACW	2 cut(s)	101, 389
TfiI	GAWTC	2 cut(s)	214, 581
Tru1I	TTAA	4 cut(s)	54, 135, 306, 612
Tru9I	TTAA	4 cut(s)	54, 135, 306, 612
TscAI	CASTG	3 cut(s)	559, 796, 820
TseFI	GTSAC	2 cut(s)	191, 827
TseI	GCWGC	4 cut(s)	77, 152, 719, 863
Tsp45I	GTSAC	2 cut(s)	191, 827
TspDTI	ATGAA	4 cut(s)	467, 911, 956, 1130
TspGWI	ACGGA	1 cut(s)	565
TspRI	CASTG	3 cut(s)	559, 796, 820
VpaK11BI	GGWCC	2 cut(s)	4, 431
VspI	ATTAAT	1 cut(s)	306
XapI	RAATTY	3 cut(s)	139, 614, 646
XceI	RCATGY	3 cut(s)	223, 390, 778
XhoI	CTCGAG	1 cut(s)	1043
XmnI	GAANNNNTTC	2 cut(s)	275, 1127
XspI	CTAG	3 cut(s)	357, 515, 992

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.