Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001777

Physical Location & Seq

Genomic Coordinates Forward (+)

21398511 .. 21403259

Gene Structure 4749 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1253600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGATTCAGGATATTTATATCCATCCTTGCCTCTCTCTCCCCCAAAACCCCATTTGTGTTTTTCTTTTTTGGACTGACCCCCCGCTGTGTTCACCAGAGGACAGAGTATCTGATGTCGAAATATCTGAGGAAGATAGAAAGACTAAAGTCGGGTCGTTGAAGAAGAAGGCAATGAATGCCTCTACTAGGTTCAGAAATTCTTTGTCAAAAAGGAATAGAAGAAGCAGTAGTAAAGTCTTGTCTATCGACATTGAGGATGTGCACGACTTAGAGGAGATACATGCTGTTGATTCGTTGCGGCAGGCACTTTTACTGGAGGAGCTTCTACCCTCCAAGCACGATGATTATCATAAGATGCTCAGATTCTTGAAGGCCAGGAAATTTGATGTCAACAAAACCAAGCAAATGTGGTCTGACATGCTCGAGTGGAGGAAGGCATTCGATGCTGACACAATCATGGAGGACTTTGAATTCAAGGAACTCGATGAAGTCTTGAAATACTACCCCCAAGGACATCATGGTGTAGACAAGGAAGGACGACCTGTGTACATCGAGAGACTAGGCCTAGTAGATGTGACCAAGCTGATGCAAGCCACAACTATGGACCGCTACGTCAAATACCATGTCCAGGAGTTTGAGAGGACATTTGCTGTTAAATTTCCAGCTTGCTCAATTTCAGCAAAGAAGCACATTGATCAAAGTACAACTATCCTCGATGTGCAAGGAGTGGGATTTAAGAATTTCAACAAATCTGCCAGGGAACTTATTACTCGCCTTCAGAAGATTGACGGTGACAATTATCCTGAGACCTTGAATCGCATGTTTATCATCAACGCTGGTTCTGGCTTTCGAATGTTGTGGAACACCGTGAAGTCCTTCCTTGATCCGAAGACTACGGCAAAGATTAATGTCCTTGGCAACAAGTATCAGAGCAAGTTGCTTGAAATAATTGATGCTAGCGAATTACCCGAGTTTTTAGGAGGTACATGTACTTGTGCTGATCAAGGAGGCTGTATGCTTTCGGACAAGGGACCCTGGAAACAACCTGAGATCATGAAGATGGTCCAAAACGGCAGTCATCAATGTTCAAGAAAATCTGGACTTCAAGGACTCGATGCAGCTCTGAATGTTGGACCTTCCGCTCCTAAACTCAATCATGCACTTTCTCGCTTCCATGAAGAAGTATCCCTAGCCAAAACTCGCCAAGTGTCTTTCAGGCGCAAGGACAGTGTCTCGATGGTTGGTAAATCTGTGGATTCGAGTTGGCCAATGTCAGTGGGGAATGTTAAATTTGCCCTCTCAAAGGCTAAATCATCTGAGAATTTAGCAGGAGATTGTTTCACCAGGAACAATGCTTGCAAACTGCCTGAAGGTTTAGGCTCTCAGATTATGGCTGCTATTATGACGTTTCTTGTGGGCATTGTCGCCATGGTCAAACTAACACGTAGCATGCCAAGGAAGCTCACCGACTCCACCTTCTACTCCAGCACTGTCTGCGACAGTGACACAATGGTCAAAGGCCAGGGCTCTTCACACTCTGCCATCTCCGGTTCAGATCTCATGACTGTCATGAAACGCATGGCTGAGTTGGAAGAGAGAATGACTGTTTTGAACATGAAACCTGCTGCCAAGCCAGCTCAGAAGGAGGAAATGCTGAATGCTGCTCTAGGTCGGGTTGATGCCTTGGAGCAAGAGCTTATGGCAACAAAGCAGGCTCTCGAGGAGTCGCTTGCCCGGCAAGAGGAGCTCTTGGGCTCTCTTGACAAAAAGAAGAAGAAGAAGAAGAACAAGAAGAAGAAAATGTTCCATATCTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000226 GO:0003006 GO:0003674 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005628 GO:0005634 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0008150 GO:0008525 GO:0008526 GO:0009653 GO:0009987 GO:0010876 GO:0010927 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016192 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022607 GO:0030154 GO:0030427 GO:0030435 GO:0030437 GO:0031321 GO:0031322 GO:0032153 GO:0032502 GO:0032505 GO:0032989 GO:0033036 GO:0034293 GO:0035838 GO:0042763 GO:0042764 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043934 GO:0043935 GO:0044085 GO:0044424 GO:0044464 GO:0044703 GO:0048193 GO:0048468 GO:0048646 GO:0048856 GO:0048869 GO:0051179 GO:0051234 GO:0051286 GO:0051321 GO:0051704 GO:0060187 GO:0061024 GO:0071702 GO:0071840 GO:0120009 GO:0120010 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO CRAL_TRIO_N

Protein Analysis

606

Amino Acids

68.36

Weight (kDa)

9.1

Isoelectric Point (pI)

47.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CRAL_TRIO_N	PF03765	113 - 138	6.8e-06	CRAL/TRIO, N-terminal domain
CRAL_TRIO	PF00650	166 - 328	2.2e-31	CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001777)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18180 AT2G18180 AT2G18180 AT2G18180 AT2G18180 AT4G36490
fragaria_vesca	FvH4_1g03070 FvH4_1g12640
malus_domestica	MD02G1140800.v1.1 MD15G1253600.v1.1
prunus_persica	Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G160200_v2.0.a1 Prupe.7G244900_v2.0.a1
pyrus_communis	pycom02g11090 pycom15g22320
rosa_chinensis	RchiOBHm_Chr2g0088201 RchiOBHm_Chr2g0100591 RchiOBHm_Chr2g0100601
rosa_laevigata	RLG00000015931
rosa_multiflora	Rmu_sc0001497.1_g000001 Rmu_sc0003275.1_g000070
rosa_roxburghii	Rroxscaffold_2G00142100 Rroxscaffold_7G00200090
rosa_rugosa	Rorug01G0481700 Rorug02G0089800 Rorug02G0089900 Rorug02G0090000 Rorug02G0090100 Rorug06G0039000 Rorug06G0389100
rosa_samantha	Rh2AG036800 Rh2AG139000 Rh2BG036000 Rh2BG142300 Rh2CG037100 Rh2CG144600 Rh2DG036500 Rh2DG144000
rosa_wichuraiana	Rw2G002900 Rw2G010890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	611, 1511
Acc36I	ACCTGC	1 cut(s)	1630
AccBSI	CCGCTC	1 cut(s)	1142
AccI	GTMKAC	1 cut(s)	525
AciI	CCGC	4 cut(s)	83, 298, 607, 1140
AclWI	GGATC	1 cut(s)	878
AcoI	YGGCCR	1 cut(s)	1265
AcsI	RAATTY	7 cut(s)	196, 380, 470, 656, 739, 1289, 1321
AcuI	CTGAAG	2 cut(s)	761, 1389
AfaI	GTAC	4 cut(s)	548, 703, 985, 991
AfiI	CCNNNNNNNGG	4 cut(s)	186, 628, 1303, 1741
AflIII	ACRYGT	2 cut(s)	986, 1442
AjnI	CCWGG	6 cut(s)	374, 627, 755, 1034, 1343, 1521
AluBI	AGCT	8 cut(s)	322, 583, 665, 1121, 1462, 1637, 1696, 1747
AluI	AGCT	8 cut(s)	322, 583, 665, 1121, 1462, 1637, 1696, 1747
Alw21I	GWGCWC	2 cut(s)	264, 1749
Alw26I	GTCTC	3 cut(s)	550, 800, 1237
Alw44I	GTGCAC	1 cut(s)	260
AlwI	GGATC	1 cut(s)	878
Ama87I	CYCGRG	3 cut(s)	422, 968, 1718
AoxI	GGCC	4 cut(s)	372, 562, 1265, 1519
ApaLI	GTGCAC	1 cut(s)	260
ApeKI	GCWGC	4 cut(s)	1118, 1394, 1625, 1661
ApoI	RAATTY	7 cut(s)	196, 380, 470, 656, 739, 1289, 1321
ArsI	GACNNNNNNTTYG	4 cut(s)	609, 641, 1060, 1092
AseI	ATTAAT	1 cut(s)	906
Asp700I	GAANNNNTTC	3 cut(s)	437, 875, 1802
AspLEI	GCGC	1 cut(s)	1221
AspS9I	GGNCC	4 cut(s)	604, 1031, 1063, 1133
AsuC2I	CCSGG	1 cut(s)	1735
AsuHPI	GGTGA	4 cut(s)	84, 803, 1333, 1456
AsuII	TTCGAA	1 cut(s)	850
AsuNHI	GCTAGC	1 cut(s)	956
AvaI	CYCGRG	3 cut(s)	422, 968, 1718
AvaII	GGWCC	4 cut(s)	604, 1031, 1063, 1133
BaeGI	GKGCMC	1 cut(s)	264
BalI	TGGCCA	1 cut(s)	1267
BanII	GRGCYC	3 cut(s)	1529, 1749, 1757
BbsI	GAAGAC	1 cut(s)	896
Bbv12I	GWGCWC	2 cut(s)	264, 1749
BbvI	GCAGC	4 cut(s)	1130, 1381, 1612, 1648
BccI	CCATC	4 cut(s)	30, 1054, 1231, 1550
BceAI	ACGGC	2 cut(s)	912, 1087
BciT130I	CCWGG	6 cut(s)	376, 629, 757, 1036, 1345, 1523
BciVI	GTATCC	1 cut(s)	1195
BclI	TGATCA	2 cut(s)	694, 1000
BcnI	CCSGG	1 cut(s)	1735
BcoDI	GTCTC	3 cut(s)	550, 800, 1237
BfaI	CTAG	6 cut(s)	186, 560, 566, 957, 1190, 1667
BfuAI	ACCTGC	1 cut(s)	1630
BfuI	GTATCC	1 cut(s)	1195
BglII	AGATCT	1 cut(s)	1555
BisI	GCNGC	5 cut(s)	299, 1119, 1395, 1626, 1662
BlsI	GCNGC	5 cut(s)	300, 1120, 1396, 1627, 1663
Bme1390I	CCNGG	7 cut(s)	376, 629, 757, 1036, 1345, 1523, 1735
Bme18I	GGWCC	4 cut(s)	604, 1031, 1063, 1133
BmeT110I	CYCGRG	3 cut(s)	422, 968, 1718
BmgT120I	GGNCC	4 cut(s)	604, 1031, 1063, 1133
BmiI	GGNNCC	2 cut(s)	1032, 1033
BmrFI	CCNGG	7 cut(s)	376, 629, 757, 1036, 1345, 1523, 1735
BmsI	GCATC	6 cut(s)	345, 433, 576, 943, 1105, 1669
BmtI	GCTAGC	1 cut(s)	960
BoxI	GACNNNNGTC	1 cut(s)	146
BpiI	GAAGAC	1 cut(s)	896
BpmI	CTGGAG	2 cut(s)	335, 1468
Bpu14I	TTCGAA	1 cut(s)	850
BpuMI	CCSGG	1 cut(s)	1735
BsaAI	YACGTR	1 cut(s)	1445
BsaBI	GATNNNNATC	1 cut(s)	345
BsaI	GGTCTC	1 cut(s)	800
BsaJI	CCNNGG	8 cut(s)	508, 756, 913, 1034, 1428, 1454, 1522, 1683
BsaWI	WCCGGW	1 cut(s)	1547
Bsc4I	CCNNNNNNNGG	4 cut(s)	186, 628, 1303, 1741
Bse1I	ACTGG	1 cut(s)	318
Bse3DI	GCAATG	1 cut(s)	177
Bse8I	GATNNNNATC	1 cut(s)	345
BseBI	CCWGG	6 cut(s)	376, 629, 757, 1036, 1345, 1523
BseDI	CCNNGG	8 cut(s)	508, 756, 913, 1034, 1428, 1454, 1522, 1683
BseGI	GGATG	2 cut(s)	22, 262
BseJI	GATNNNNATC	1 cut(s)	345
BseLI	CCNNNNNNNGG	4 cut(s)	186, 628, 1303, 1741
BseMI	GCAATG	1 cut(s)	177
BseMII	CTCAG	8 cut(s)	117, 373, 795, 1038, 1308, 1397, 1575, 1652
BseNI	ACTGG	1 cut(s)	318
BseRI	GAGGAG	4 cut(s)	287, 332, 1736, 1757
BseSI	GKGCMC	1 cut(s)	264
BseXI	GCAGC	4 cut(s)	1130, 1381, 1612, 1648
BshFI	GGCC	4 cut(s)	374, 564, 1267, 1521
BsiHKAI	GWGCWC	2 cut(s)	264, 1749
BsiHKCI	CYCGRG	3 cut(s)	422, 968, 1718
BsiSI	CCGG	2 cut(s)	1548, 1735
BslFI	GGGAC	1 cut(s)	1044
BslI	CCNNNNNNNGG	4 cut(s)	186, 628, 1303, 1741
BsmAI	GTCTC	3 cut(s)	550, 800, 1237
BsmFI	GGGAC	1 cut(s)	1044
BsmI	GAATGC	3 cut(s)	181, 437, 1663
BsnI	GGCC	4 cut(s)	374, 564, 1267, 1521
Bso31I	GGTCTC	1 cut(s)	800
BsoBI	CYCGRG	3 cut(s)	422, 968, 1718
Bsp119I	TTCGAA	1 cut(s)	850
Bsp1286I	GDGCHC	4 cut(s)	264, 1529, 1749, 1757
Bsp1407I	TGTACA	1 cut(s)	546
Bsp143I	GATC	5 cut(s)	694, 883, 1000, 1050, 1555
Bsp19I	CCATGG	1 cut(s)	1428
BspACI	CCGC	4 cut(s)	83, 298, 607, 1140
BspANI	GGCC	4 cut(s)	374, 564, 1267, 1521
BspCNI	CTCAG	8 cut(s)	118, 372, 796, 1039, 1309, 1396, 1576, 1651
BspHI	TCATGA	3 cut(s)	1053, 1560, 1569
BspLI	GGNNCC	2 cut(s)	1032, 1033
BspMI	ACCTGC	1 cut(s)	1630
BspOI	GCTAGC	1 cut(s)	960
BspPI	GGATC	1 cut(s)	878
BspQI	GCTCTTC	1 cut(s)	1534
BspT104I	TTCGAA	1 cut(s)	850
BspTNI	GGTCTC	1 cut(s)	800
BsrBI	CCGCTC	1 cut(s)	1142
BsrDI	GCAATG	1 cut(s)	177
BsrGI	TGTACA	1 cut(s)	546
BsrI	ACTGG	1 cut(s)	318
BssECI	CCNNGG	8 cut(s)	508, 756, 913, 1034, 1428, 1454, 1522, 1683
BssMI	GATC	5 cut(s)	694, 883, 1000, 1050, 1555
BssT1I	CCWWGG	5 cut(s)	508, 913, 1428, 1454, 1683
Bst2UI	CCWGG	6 cut(s)	376, 629, 757, 1036, 1345, 1523
Bst4CI	ACNGT	7 cut(s)	791, 868, 1229, 1492, 1502, 1567, 1606
Bst6I	CTCTTC	2 cut(s)	1534, 1587
BstAPI	GCANNNNNTGC	3 cut(s)	176, 443, 1494
BstAUI	TGTACA	1 cut(s)	546
BstBAI	YACGTR	1 cut(s)	1445
BstBI	TTCGAA	1 cut(s)	850
BstC8I	GCNNGC	9 cut(s)	303, 591, 667, 958, 1357, 1451, 1635, 1713, 1731
BstDEI	CTNAG	9 cut(s)	126, 268, 359, 804, 1047, 1317, 1383, 1584, 1638
BstDSI	CCRYGG	1 cut(s)	1428
BstENI	CCTNNNNNAGG	2 cut(s)	184, 1301
BstF5I	GGATG	2 cut(s)	22, 262
BstHHI	GCGC	1 cut(s)	1221
BstKTI	GATC	5 cut(s)	697, 886, 1003, 1053, 1558
BstMAI	GTCTC	3 cut(s)	550, 800, 1237
BstMBI	GATC	5 cut(s)	694, 883, 1000, 1050, 1555
BstMWI	GCNNNNNNNGC	7 cut(s)	176, 443, 1459, 1494, 1634, 1735, 1744
BstNI	CCWGG	6 cut(s)	376, 629, 757, 1036, 1345, 1523
BstNSI	RCATGY	5 cut(s)	284, 421, 823, 990, 1453
BstPAI	GACNNNNGTC	1 cut(s)	146
BstSCI	CCNGG	7 cut(s)	374, 627, 755, 1034, 1343, 1521, 1733
BstSLI	GKGCMC	1 cut(s)	264
BstV1I	GCAGC	4 cut(s)	1130, 1381, 1612, 1648
BstV2I	GAAGAC	1 cut(s)	896
BstX2I	RGATCY	1 cut(s)	1555
BstXI	CCANNNNNNTGG	1 cut(s)	601
BstYI	RGATCY	1 cut(s)	1555
BsuI	GTATCC	1 cut(s)	1195
BsuRI	GGCC	4 cut(s)	374, 564, 1267, 1521
BtgI	CCRYGG	1 cut(s)	1428
BtsCI	GGATG	2 cut(s)	22, 262
BtsIMutI	CAGTG	4 cut(s)	1234, 1281, 1488, 1507
BveI	ACCTGC	1 cut(s)	1630
Cac8I	GCNNGC	9 cut(s)	303, 591, 667, 958, 1357, 1451, 1635, 1713, 1731
CciI	TCATGA	3 cut(s)	1053, 1560, 1569
CfoI	GCGC	1 cut(s)	1221
Cfr13I	GGNCC	4 cut(s)	604, 1031, 1063, 1133
Csp6I	GTAC	4 cut(s)	547, 702, 984, 990
CspCI	CAANNNNNGTGG	2 cut(s)	1257, 1292
CviQI	GTAC	4 cut(s)	547, 702, 984, 990
DdeI	CTNAG	9 cut(s)	126, 268, 359, 804, 1047, 1317, 1383, 1584, 1638
DpnI	GATC	5 cut(s)	696, 885, 1002, 1052, 1557
DpnII	GATC	5 cut(s)	694, 883, 1000, 1050, 1555
DrdI	GACNNNNNNGTC	2 cut(s)	611, 1511
DseDI	GACNNNNNNGTC	2 cut(s)	611, 1511
EaeI	YGGCCR	1 cut(s)	1265
Eam1104I	CTCTTC	2 cut(s)	1534, 1587
EarI	CTCTTC	2 cut(s)	1534, 1587
Ecl136II	GAGCTC	1 cut(s)	1747
Eco130I	CCWWGG	5 cut(s)	508, 913, 1428, 1454, 1683
Eco147I	AGGCCT	1 cut(s)	564
Eco24I	GRGCYC	3 cut(s)	1529, 1749, 1757
Eco31I	GGTCTC	1 cut(s)	800
Eco47I	GGWCC	4 cut(s)	604, 1031, 1063, 1133
Eco53kI	GAGCTC	1 cut(s)	1747
Eco57I	CTGAAG	2 cut(s)	761, 1389
Eco88I	CYCGRG	3 cut(s)	422, 968, 1718
EcoICRI	GAGCTC	1 cut(s)	1747
EcoNI	CCTNNNNNAGG	2 cut(s)	184, 1301
EcoO109I	RGGNCCY	1 cut(s)	1031
EcoRI	GAATTC	1 cut(s)	470
EcoRII	CCWGG	6 cut(s)	374, 627, 755, 1034, 1343, 1521
EcoT14I	CCWWGG	5 cut(s)	508, 913, 1428, 1454, 1683
EcoT38I	GRGCYC	3 cut(s)	1529, 1749, 1757
ErhI	CCWWGG	5 cut(s)	508, 913, 1428, 1454, 1683
FaqI	GGGAC	1 cut(s)	1044
FauI	CCCGC	1 cut(s)	90
FbaI	TGATCA	2 cut(s)	694, 1000
FblI	GTMKAC	1 cut(s)	525
Fnu4HI	GCNGC	5 cut(s)	299, 1119, 1395, 1626, 1662
FokI	GGATG	2 cut(s)	9, 269
FriOI	GRGCYC	3 cut(s)	1529, 1749, 1757
Fsp4HI	GCNGC	5 cut(s)	299, 1119, 1395, 1626, 1662
FspBI	CTAG	6 cut(s)	186, 560, 566, 957, 1190, 1667
GlaI	GCGC	1 cut(s)	1220
GluI	GCNGC	5 cut(s)	299, 1119, 1395, 1626, 1662
GsuI	CTGGAG	2 cut(s)	335, 1468
HaeIII	GGCC	4 cut(s)	374, 564, 1267, 1521
HapII	CCGG	2 cut(s)	1548, 1735
HhaI	GCGC	1 cut(s)	1221
Hin6I	GCGC	1 cut(s)	1219
HinP1I	GCGC	1 cut(s)	1219
HincII	GTYRAC	1 cut(s)	391
HindII	GTYRAC	1 cut(s)	391
HinfI	GANTC	8 cut(s)	4, 290, 363, 814, 1110, 1256, 1469, 1724
HpaII	CCGG	2 cut(s)	1548, 1735
HphI	GGTGA	4 cut(s)	84, 803, 1333, 1456
Hpy166II	GTNNAC	5 cut(s)	92, 262, 391, 526, 547
Hpy8I	GTNNAC	5 cut(s)	92, 262, 391, 526, 547
HpyCH4III	ACNGT	7 cut(s)	791, 868, 1229, 1492, 1502, 1567, 1606
HpyCH4IV	ACGT	3 cut(s)	612, 1406, 1444
HpyCH4V	TGCA	6 cut(s)	262, 589, 721, 1118, 1160, 1359
HpyF10VI	GCNNNNNNNGC	7 cut(s)	176, 443, 1459, 1494, 1634, 1735, 1744
HpyF3I	CTNAG	9 cut(s)	126, 268, 359, 804, 1047, 1317, 1383, 1584, 1638
HpySE526I	ACGT	3 cut(s)	612, 1406, 1444
HspAI	GCGC	1 cut(s)	1219
KflI	GGGWCCC	1 cut(s)	1031
Ksp22I	TGATCA	2 cut(s)	694, 1000
Kzo9I	GATC	5 cut(s)	694, 883, 1000, 1050, 1555
LguI	GCTCTTC	1 cut(s)	1534
LmnI	GCTCC	4 cut(s)	319, 1147, 1687, 1744
Lsp1109I	GCAGC	4 cut(s)	1130, 1381, 1612, 1648
LweI	GCATC	6 cut(s)	345, 433, 576, 943, 1105, 1669
MaeI	CTAG	6 cut(s)	186, 560, 566, 957, 1190, 1667
MaeII	ACGT	3 cut(s)	612, 1406, 1444
MaeIII	GTNAC	3 cut(s)	574, 791, 1502
MalI	GATC	5 cut(s)	696, 885, 1002, 1052, 1557
MbiI	CCGCTC	1 cut(s)	1142
MboI	GATC	5 cut(s)	694, 883, 1000, 1050, 1555
MflI	RGATCY	1 cut(s)	1555
MhlI	GDGCHC	4 cut(s)	264, 1529, 1749, 1757
MlsI	TGGCCA	1 cut(s)	1267
MluNI	TGGCCA	1 cut(s)	1267
MlyI	GAGTC	3 cut(s)	1104, 1463, 1733
MmeI	TCCRAC	2 cut(s)	1111, 1569
Mox20I	TGGCCA	1 cut(s)	1267
MroXI	GAANNNNTTC	3 cut(s)	437, 875, 1802
MscI	TGGCCA	1 cut(s)	1267
MseI	TTAA	4 cut(s)	654, 735, 906, 1287
MslI	CAYNNNNRTG	4 cut(s)	455, 519, 599, 1058
Msp20I	TGGCCA	1 cut(s)	1267
MspA1I	CMGCKG	1 cut(s)	85
MspI	CCGG	2 cut(s)	1548, 1735
MspR9I	CCNGG	7 cut(s)	376, 629, 757, 1036, 1345, 1523, 1735
Mva1269I	GAATGC	3 cut(s)	181, 437, 1663
MvaI	CCWGG	6 cut(s)	376, 629, 757, 1036, 1345, 1523
MwoI	GCNNNNNNNGC	7 cut(s)	176, 443, 1459, 1494, 1634, 1735, 1744
NciI	CCSGG	1 cut(s)	1735
NcoI	CCATGG	1 cut(s)	1428
NdeII	GATC	5 cut(s)	694, 883, 1000, 1050, 1555
NheI	GCTAGC	1 cut(s)	956
NlaIV	GGNNCC	2 cut(s)	1032, 1033
NmuCI	GTSAC	3 cut(s)	574, 791, 1502
NspI	RCATGY	5 cut(s)	284, 421, 823, 990, 1453
NspV	TTCGAA	1 cut(s)	850
PaeI	GCATGC	1 cut(s)	1453
PaeR7I	CTCGAG	2 cut(s)	422, 1718
PagI	TCATGA	3 cut(s)	1053, 1560, 1569
PceI	AGGCCT	1 cut(s)	564
PciI	ACATGT	1 cut(s)	986
PciSI	GCTCTTC	1 cut(s)	1534
PctI	GAATGC	3 cut(s)	181, 437, 1663
PdmI	GAANNNNTTC	3 cut(s)	437, 875, 1802
PfeI	GAWTC	5 cut(s)	4, 290, 363, 814, 1256
PflFI	GACNNNGTC	1 cut(s)	1229
PfoI	TCCNGGA	1 cut(s)	627
PkrI	GCNGC	5 cut(s)	300, 1120, 1396, 1627, 1663
PleI	GAGTC	3 cut(s)	1104, 1463, 1732
PpsI	GAGTC	3 cut(s)	1104, 1463, 1732
Ppu21I	YACGTR	1 cut(s)	1445
PpuMI	RGGWCCY	1 cut(s)	1031
PscI	ACATGT	1 cut(s)	986
PshAI	GACNNNNGTC	1 cut(s)	146
PshBI	ATTAAT	1 cut(s)	906
Psp124BI	GAGCTC	1 cut(s)	1749
Psp5II	RGGWCCY	1 cut(s)	1031
Psp6I	CCWGG	6 cut(s)	374, 627, 755, 1034, 1343, 1521
PspGI	CCWGG	6 cut(s)	374, 627, 755, 1034, 1343, 1521
PspN4I	GGNNCC	2 cut(s)	1032, 1033
PspPI	GGNCC	4 cut(s)	604, 1031, 1063, 1133
PspPPI	RGGWCCY	1 cut(s)	1031
PspXI	VCTCGAGB	1 cut(s)	422
PsuI	RGATCY	1 cut(s)	1555
PsyI	GACNNNGTC	1 cut(s)	1229
RsaI	GTAC	4 cut(s)	548, 703, 985, 991
RsaNI	GTAC	4 cut(s)	547, 702, 984, 990
RseI	CAYNNNNRTG	4 cut(s)	455, 519, 599, 1058
SacI	GAGCTC	1 cut(s)	1749
SapI	GCTCTTC	1 cut(s)	1534
SaqAI	TTAA	4 cut(s)	654, 735, 906, 1287
SatI	GCNGC	5 cut(s)	299, 1119, 1395, 1626, 1662
Sau3AI	GATC	5 cut(s)	694, 883, 1000, 1050, 1555
Sau96I	GGNCC	4 cut(s)	604, 1031, 1063, 1133
SchI	GAGTC	3 cut(s)	1104, 1463, 1733
ScrFI	CCNGG	7 cut(s)	376, 629, 757, 1036, 1345, 1523, 1735
SduI	GDGCHC	4 cut(s)	264, 1529, 1749, 1757
SfaNI	GCATC	6 cut(s)	345, 433, 576, 943, 1105, 1669
Sfr274I	CTCGAG	2 cut(s)	422, 1718
SfuI	TTCGAA	1 cut(s)	850
SinI	GGWCC	4 cut(s)	604, 1031, 1063, 1133
SlaI	CTCGAG	2 cut(s)	422, 1718
SmiMI	CAYNNNNRTG	4 cut(s)	455, 519, 599, 1058
SmlI	CTYRAG	2 cut(s)	422, 1718
SmoI	CTYRAG	2 cut(s)	422, 1718
SphI	GCATGC	1 cut(s)	1453
SseBI	AGGCCT	1 cut(s)	564
SsiI	CCGC	4 cut(s)	83, 298, 607, 1140
SspMI	CTAG	6 cut(s)	186, 560, 566, 957, 1190, 1667
SstI	GAGCTC	1 cut(s)	1749
StuI	AGGCCT	1 cut(s)	564
StyD4I	CCNGG	7 cut(s)	374, 627, 755, 1034, 1343, 1521, 1733
StyI	CCWWGG	5 cut(s)	508, 913, 1428, 1454, 1683
TaaI	ACNGT	7 cut(s)	791, 868, 1229, 1492, 1502, 1567, 1606
TaiI	ACGT	3 cut(s)	615, 1409, 1447
TatI	WGTACW	3 cut(s)	546, 701, 989
TauI	GCSGC	1 cut(s)	301
TfiI	GAWTC	5 cut(s)	4, 290, 363, 814, 1256
Tru1I	TTAA	4 cut(s)	654, 735, 906, 1287
Tru9I	TTAA	4 cut(s)	654, 735, 906, 1287
TscAI	CASTG	4 cut(s)	1234, 1281, 1495, 1507
TseFI	GTSAC	3 cut(s)	574, 791, 1502
TseI	GCWGC	4 cut(s)	1118, 1394, 1625, 1661
Tsp45I	GTSAC	3 cut(s)	574, 791, 1502
TspDTI	ATGAA	6 cut(s)	188, 501, 1070, 1191, 1586, 1631
TspRI	CASTG	4 cut(s)	1234, 1281, 1495, 1507
Tth111I	GACNNNGTC	1 cut(s)	1229
VneI	GTGCAC	1 cut(s)	260
VpaK11BI	GGWCC	4 cut(s)	604, 1031, 1063, 1133
VspI	ATTAAT	1 cut(s)	906
XagI	CCTNNNNNAGG	2 cut(s)	184, 1301
XapI	RAATTY	7 cut(s)	196, 380, 470, 656, 739, 1289, 1321
XceI	RCATGY	5 cut(s)	284, 421, 823, 990, 1453
XcmI	CCANNNNNNNNNTGG	1 cut(s)	515
XhoI	CTCGAG	2 cut(s)	422, 1718
XmiI	GTMKAC	1 cut(s)	525
XmnI	GAANNNNTTC	3 cut(s)	437, 875, 1802
XspI	CTAG	6 cut(s)	186, 560, 566, 957, 1190, 1667

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.