MD02G1166900.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008830
Physical Location & Seq
Reverse (-)
14336403 .. 14340366
3964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1166900.v1.1.491

Sequence Viewer

Length: 918 bp
ATGTCGTCGTCCTCAATGTCGTCGTCTTCAAACTCTCCGTGCGCAGCGTGCAAATTTCTTCGGCGGAAGTGCACGCCGGAGTGCGTCTTCGCGCCTTACTTCCCACCTGACAACCCCCAAAGGTTTGCCAACGTCCACAAGGTCTTCGGCGCCAGCAATGTCGCCAAGATCCTCAACGAGCTCAGCGCTTCTCAGCGCGATGACGCCGTCAACTCCCTGGCCTACGAGGCGGAGGCCCGCCTCCGGGACCCTGTCTATGGCTGCGTCGGACTCATCTCCCTCCTCCAGCAGCGGCTGAAGCAAGTCCAGGCGGACCTTTTCAATGCCAAAAAGGAGCTCGCAACTTACATAGGTCCACAAGCTATGCTCTCCATTCTCCAGCCGCCGACTTACATGGCTCAGCAGCTCCAAGGAAACGCTTCTTCCTCGGCCGGTCCGCCGTACAGCATGTCTCCGATGATGGGCATTCCGACTGCTGCAGCTTCCGGTCAGCTGATGATTCGAGATCCCCAGCAGCAGCAACAGCATCAACAGATTTTCGAGGCGCAGCAGTTGGCGGCAGCCGTGGCGGGGAGGGAGCAGCAGGAGATGTTCAGGAGTTTTGATCAGAGTAAACAGGCAATGCAGCAGCAGGCGCAGGACCATTTGAGCGGGTTCACTCAGATGGCGGGAGCGGTGGGCAACGCTGTTTTGCCGTCTTTGGCTCTGGGGTTTGATAGCGGTCCGTACCACCAGATTCAGCCGCAGCGCCCTCACCATAATCACCGCCACCAGCTGCAGCTTCAGGCTCAGCTGTTGCTCCAAACCCAACAGCCGCAAGGGCAGCATCGGGCCCAGCAATCCCAGCAACCCCAGCTGCAGGCAAAACCCGATCCGGGCGAGGAAGGTGGTGGTAGGAGTGTGGGTCCCTCTTGTTGA

Protein Analysis

306

Amino Acids

33.28

Weight (kDa)

8.53

Isoelectric Point (pI)

72.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 13 - 110 1e-41 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 149
AccBSI CCGCTC 2 cut(s) 651, 674
AccII CGCG 2 cut(s) 92, 198
AclWI GGATC 3 cut(s) 163, 500, 866
AcoI YGGCCR 1 cut(s) 429
AcsI RAATTY 1 cut(s) 53
AcuI CTGAAG 2 cut(s) 317, 767
AcyI GRCGYC 2 cut(s) 150, 204
AfaI GTAC 2 cut(s) 443, 728
AfeI AGCGCT 1 cut(s) 187
AfiI CCNNNNNNNGG 7 cut(s) 243, 244, 257, 461, 570, 859, 875
AgsI TTSAA 2 cut(s) 30, 322
AjnI CCWGG 2 cut(s) 216, 306
Alw21I GWGCWC 3 cut(s) 74, 183, 339
Alw26I GTCTC 1 cut(s) 456
Alw44I GTGCAC 1 cut(s) 70
AlwI GGATC 3 cut(s) 163, 500, 866
AlwNI CAGNNNCTG 1 cut(s) 295
Aor51HI AGCGCT 1 cut(s) 187
AoxI GGCC 4 cut(s) 219, 234, 429, 831
ApaI GGGCCC 1 cut(s) 835
ApaLI GTGCAC 1 cut(s) 70
ApoI RAATTY 1 cut(s) 53
Asp700I GAANNNNTTC 1 cut(s) 418
AspLEI GCGC 8 cut(s) 44, 94, 152, 188, 198, 547, 637, 750
AsuC2I CCSGG 2 cut(s) 245, 876
AsuHPI GGTGA 2 cut(s) 746, 755
AvaII GGWCC 7 cut(s) 247, 313, 353, 434, 640, 722, 905
BaeGI GKGCMC 2 cut(s) 74, 835
BanI GGYRCC 1 cut(s) 149
BanII GRGCYC 3 cut(s) 183, 339, 835
BbsI GAAGAC 3 cut(s) 18, 79, 136
Bbv12I GWGCWC 3 cut(s) 74, 183, 339
BccI CCATC 2 cut(s) 454, 658
BceAI ACGGC 4 cut(s) 191, 424, 548, 679
BciT130I CCWGG 2 cut(s) 218, 308
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 2 cut(s) 245, 876
BcoDI GTCTC 1 cut(s) 456
BfmI CTRYAG 3 cut(s) 477, 776, 857
BfoI RGCGCY 3 cut(s) 153, 189, 751
BglI GCCNNNNNGGC 2 cut(s) 227, 820
BlpI GCTNAGC 3 cut(s) 182, 399, 789
Bme1390I CCNGG 4 cut(s) 218, 245, 308, 876
Bme18I GGWCC 7 cut(s) 247, 313, 353, 434, 640, 722, 905
BmiI GGNNCC 6 cut(s) 151, 248, 249, 833, 906, 907
BmrFI CCNGG 4 cut(s) 218, 245, 308, 876
BmsI GCATC 2 cut(s) 535, 835
BpiI GAAGAC 3 cut(s) 18, 79, 136
BpmI CTGGAG 2 cut(s) 269, 362
Bpu1102I GCTNAGC 3 cut(s) 182, 399, 789
BpuMI CCSGG 2 cut(s) 245, 876
BsaHI GRCGYC 2 cut(s) 150, 204
BsaJI CCNNGG 4 cut(s) 216, 409, 426, 564
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 1 cut(s) 889
Bsc4I CCNNNNNNNGG 7 cut(s) 243, 244, 257, 461, 570, 859, 875
Bse118I RCCGGY 1 cut(s) 431
Bse3DI GCAATG 2 cut(s) 163, 627
BseBI CCWGG 2 cut(s) 218, 308
BseDI CCNNGG 4 cut(s) 216, 409, 426, 564
BseLI CCNNNNNNNGG 7 cut(s) 243, 244, 257, 461, 570, 859, 875
BseMI GCAATG 2 cut(s) 163, 627
BseMII CTCAG 5 cut(s) 196, 206, 413, 674, 803
BseRI GAGGAG 1 cut(s) 272
BseSI GKGCMC 2 cut(s) 74, 835
BseX3I CGGCCG 1 cut(s) 429
BseYI CCCAGC 4 cut(s) 510, 834, 843, 852
Bsh1236I CGCG 2 cut(s) 92, 198
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 4 cut(s) 221, 236, 431, 833
BshNI GGYRCC 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 3 cut(s) 74, 183, 339
BsiSI CCGG 5 cut(s) 77, 244, 432, 486, 875
BslFI GGGAC 2 cut(s) 260, 891
BslI CCNNNNNNNGG 7 cut(s) 243, 244, 257, 461, 570, 859, 875
BsmAI GTCTC 1 cut(s) 456
BsmFI GGGAC 2 cut(s) 260, 891
BsmI GAATGC 1 cut(s) 465
BsnI GGCC 4 cut(s) 221, 236, 431, 833
Bsp120I GGGCCC 1 cut(s) 831
Bsp1286I GDGCHC 4 cut(s) 74, 183, 339, 835
Bsp143I GATC 4 cut(s) 168, 505, 604, 871
Bsp1720I GCTNAGC 3 cut(s) 182, 399, 789
BspANI GGCC 4 cut(s) 221, 236, 431, 833
BspCNI CTCAG 5 cut(s) 195, 205, 412, 673, 802
BspFNI CGCG 2 cut(s) 92, 198
BspLI GGNNCC 6 cut(s) 151, 248, 249, 833, 906, 907
BspMAI CTGCAG 3 cut(s) 481, 780, 861
BspPI GGATC 3 cut(s) 163, 500, 866
BspT107I GGYRCC 1 cut(s) 149
BsrBI CCGCTC 2 cut(s) 651, 674
BsrDI GCAATG 2 cut(s) 163, 627
BsrFI RCCGGY 1 cut(s) 431
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 4 cut(s) 216, 409, 426, 564
BssMI GATC 4 cut(s) 168, 505, 604, 871
BssNI GRCGYC 2 cut(s) 150, 204
BssT1I CCWWGG 1 cut(s) 409
Bst2UI CCWGG 2 cut(s) 218, 308
BstACI GRCGYC 2 cut(s) 150, 204
BstC8I GCNNGC 7 cut(s) 49, 74, 154, 238, 339, 633, 861
BstDEI CTNAG 5 cut(s) 182, 192, 399, 660, 789
BstDSI CCRYGG 1 cut(s) 564
BstFNI CGCG 2 cut(s) 92, 198
BstH2I RGCGCY 3 cut(s) 153, 189, 751
BstHHI GCGC 8 cut(s) 44, 94, 152, 188, 198, 547, 637, 750
BstKTI GATC 4 cut(s) 171, 508, 607, 874
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 4 cut(s) 168, 505, 604, 871
BstMCI CGRYCG 1 cut(s) 432
BstNI CCWGG 2 cut(s) 218, 308
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 4 cut(s) 216, 243, 306, 874
BstSFI CTRYAG 3 cut(s) 477, 776, 857
BstSLI GKGCMC 2 cut(s) 74, 835
BstUI CGCG 2 cut(s) 92, 198
BstV2I GAAGAC 3 cut(s) 18, 79, 136
BstX2I RGATCY 2 cut(s) 168, 505
BstYI RGATCY 2 cut(s) 168, 505
BstZI CGGCCG 1 cut(s) 429
BsuRI GGCC 4 cut(s) 221, 236, 431, 833
BtgI CCRYGG 1 cut(s) 564
BtgZI GCGATG 1 cut(s) 213
Cac8I GCNNGC 7 cut(s) 49, 74, 154, 238, 339, 633, 861
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 8 cut(s) 44, 94, 152, 188, 198, 547, 637, 750
Cfr10I RCCGGY 1 cut(s) 431
CpoI CGGWCCG 2 cut(s) 434, 722
CseI GACGC 3 cut(s) 73, 212, 253
Csp6I GTAC 2 cut(s) 442, 727
CspI CGGWCCG 2 cut(s) 434, 722
CviAII CATG 2 cut(s) 394, 448
CviQI GTAC 2 cut(s) 442, 727
DdeI CTNAG 5 cut(s) 182, 192, 399, 660, 789
DinI GGCGCC 1 cut(s) 151
DpnI GATC 4 cut(s) 170, 507, 606, 873
DpnII GATC 4 cut(s) 168, 505, 604, 871
EaeI YGGCCR 1 cut(s) 429
EagI CGGCCG 1 cut(s) 429
EciI GGCGGA 4 cut(s) 79, 245, 326, 426
Ecl136II GAGCTC 2 cut(s) 181, 337
EclXI CGGCCG 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 409
Eco24I GRGCYC 3 cut(s) 183, 339, 835
Eco47I GGWCC 7 cut(s) 247, 313, 353, 434, 640, 722, 905
Eco47III AGCGCT 1 cut(s) 187
Eco52I CGGCCG 1 cut(s) 429
Eco53kI GAGCTC 2 cut(s) 181, 337
Eco57I CTGAAG 2 cut(s) 317, 767
EcoICRI GAGCTC 2 cut(s) 181, 337
EcoO109I RGGNCCY 2 cut(s) 247, 905
EcoRII CCWGG 2 cut(s) 216, 306
EcoT14I CCWWGG 1 cut(s) 409
EcoT38I GRGCYC 3 cut(s) 183, 339, 835
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 2 cut(s) 397, 451
FaiI YATR 6 cut(s) 258, 350, 365, 395, 449, 759
FaqI GGGAC 2 cut(s) 260, 891
FatI CATG 2 cut(s) 393, 447
FauI CCCGC 4 cut(s) 245, 562, 644, 661
FbaI TGATCA 1 cut(s) 604
FriOI GRGCYC 3 cut(s) 183, 339, 835
FspI TGCGCA 1 cut(s) 43
GlaI GCGC 8 cut(s) 43, 93, 151, 187, 197, 546, 636, 749
GsaI CCCAGC 4 cut(s) 514, 838, 847, 856
GsuI CTGGAG 2 cut(s) 269, 362
HaeII RGCGCY 3 cut(s) 153, 189, 751
HaeIII GGCC 4 cut(s) 221, 236, 431, 833
HapII CCGG 5 cut(s) 77, 244, 432, 486, 875
HgaI GACGC 3 cut(s) 73, 212, 253
HhaI GCGC 8 cut(s) 44, 94, 152, 188, 198, 547, 637, 750
Hin1I GRCGYC 2 cut(s) 150, 204
Hin1II CATG 2 cut(s) 397, 451
Hin6I GCGC 8 cut(s) 42, 92, 150, 186, 196, 545, 635, 748
HinP1I GCGC 8 cut(s) 42, 92, 150, 186, 196, 545, 635, 748
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 3 cut(s) 270, 499, 736
HpaII CCGG 5 cut(s) 77, 244, 432, 486, 875
HphI GGTGA 2 cut(s) 746, 755
Hpy166II GTNNAC 6 cut(s) 72, 136, 211, 356, 614, 657
Hpy188I TCNGA 5 cut(s) 269, 456, 471, 609, 663
Hpy188III TCNNGA 2 cut(s) 503, 595
Hpy8I GTNNAC 6 cut(s) 72, 136, 211, 356, 614, 657
Hpy99I CGWCG 3 cut(s) 10, 25, 269
HpyAV CCTTC 1 cut(s) 878
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 6 cut(s) 51, 72, 479, 625, 778, 859
HpyF3I CTNAG 5 cut(s) 182, 192, 399, 660, 789
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 2 cut(s) 150, 204
Hsp92II CATG 2 cut(s) 397, 451
HspAI GCGC 8 cut(s) 42, 92, 150, 186, 196, 545, 635, 748
KasI GGCGCC 1 cut(s) 149
KflI GGGWCCC 2 cut(s) 247, 905
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 4 cut(s) 168, 505, 604, 871
LmnI GCTCC 5 cut(s) 334, 411, 577, 671, 804
LweI GCATC 2 cut(s) 535, 835
MaeII ACGT 1 cut(s) 132
MalI GATC 4 cut(s) 170, 507, 606, 873
MbiI CCGCTC 2 cut(s) 651, 674
MboI GATC 4 cut(s) 168, 505, 604, 871
MboII GAAGA 5 cut(s) 18, 50, 79, 136, 414
MflI RGATCY 2 cut(s) 168, 505
MhlI GDGCHC 4 cut(s) 74, 183, 339, 835
MluCI AATT 1 cut(s) 53
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 2 cut(s) 247, 494
MroXI GAANNNNTTC 1 cut(s) 418
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 5 cut(s) 292, 493, 775, 793, 856
MspI CCGG 5 cut(s) 77, 244, 432, 486, 875
MspR9I CCNGG 4 cut(s) 218, 245, 308, 876
Mva1269I GAATGC 1 cut(s) 465
MvaI CCWGG 2 cut(s) 218, 308
MvnI CGCG 2 cut(s) 92, 198
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 2 cut(s) 245, 876
NdeII GATC 4 cut(s) 168, 505, 604, 871
NlaIII CATG 2 cut(s) 397, 451
NlaIV GGNNCC 6 cut(s) 151, 248, 249, 833, 906, 907
NmeAIII GCCGAG 1 cut(s) 407
NsbI TGCGCA 1 cut(s) 43
NspI RCATGY 1 cut(s) 451
PctI GAATGC 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 2 cut(s) 499, 736
PflFI GACNNNGTC 2 cut(s) 206, 251
PfoI TCCNGGA 1 cut(s) 243
PleI GAGTC 1 cut(s) 264
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 264
PpuMI RGGWCCY 2 cut(s) 247, 905
Psp124BI GAGCTC 2 cut(s) 183, 339
Psp5II RGGWCCY 2 cut(s) 247, 905
Psp6I CCWGG 2 cut(s) 216, 306
PspFI CCCAGC 4 cut(s) 510, 834, 843, 852
PspGI CCWGG 2 cut(s) 216, 306
PspN4I GGNNCC 6 cut(s) 151, 248, 249, 833, 906, 907
PspOMI GGGCCC 1 cut(s) 831
PspPPI RGGWCCY 2 cut(s) 247, 905
PstI CTGCAG 3 cut(s) 481, 780, 861
PstNI CAGNNNCTG 1 cut(s) 295
PsuI RGATCY 2 cut(s) 168, 505
PsyI GACNNNGTC 2 cut(s) 206, 251
PvuII CAGCTG 4 cut(s) 493, 775, 793, 856
RsaI GTAC 2 cut(s) 443, 728
RsaNI GTAC 2 cut(s) 442, 727
RseI CAYNNNNRTG 1 cut(s) 662
Rsr2I CGGWCCG 2 cut(s) 434, 722
RsrII CGGWCCG 2 cut(s) 434, 722
SacI GAGCTC 2 cut(s) 183, 339
Sau3AI GATC 4 cut(s) 168, 505, 604, 871
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 4 cut(s) 218, 245, 308, 876
SduI GDGCHC 4 cut(s) 74, 183, 339, 835
SfaNI GCATC 2 cut(s) 535, 835
SfcI CTRYAG 3 cut(s) 477, 776, 857
SfoI GGCGCC 1 cut(s) 151
SinI GGWCC 7 cut(s) 247, 313, 353, 434, 640, 722, 905
SmiMI CAYNNNNRTG 1 cut(s) 662
Sse9I AATT 1 cut(s) 53
SspDI GGCGCC 1 cut(s) 149
SstI GAGCTC 2 cut(s) 183, 339
StyD4I CCNGG 4 cut(s) 216, 243, 306, 874
StyI CCWWGG 1 cut(s) 409
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 502, 540
TasI AATT 1 cut(s) 53
TauI GCSGC 5 cut(s) 295, 385, 560, 745, 817
TfiI GAWTC 2 cut(s) 499, 736
TspGWI ACGGA 2 cut(s) 27, 714
Tth111I GACNNNGTC 2 cut(s) 206, 251
VneI GTGCAC 1 cut(s) 70
VpaK11BI GGWCC 7 cut(s) 247, 313, 353, 434, 640, 722, 905
XapI RAATTY 1 cut(s) 53
XceI RCATGY 1 cut(s) 451
XmnI GAANNNNTTC 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.