Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0008830

Physical Location & Seq

Genomic Coordinates Forward (+)

10565320 .. 10570760

Gene Structure 5441 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0122800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2454 bp

ATGGAGAAGGGCAGTGTGGCCTCAGCTTTTCATGTGCTTATTGTTATTGTTGTTGTTGTTCTTATTTGCTTGTGTAACAAAGCTGTTAATGGCGGTTTTGTGAAGTACAACACAAGGAGTGGTCTGGTGGAAGGCAAACTAAATGTTCATTTGGTTGCACACTCCCATGACGACGTTGGATGGTTGAAGACTGTTGATCAGTACTATGTTGGATCAAACAACAGCATTCAGGCTGCATGTATAGAGAATGTGTTGGACTCTGTCATGGAATCGTTGGTTCGTGATCCGAATAGGAAATTTGTGTTTGCTGAGATGGCATTTTTTCAAAGATGGTGGTTAACACAAACTGTGGAAATGCAAGAACAAGTGCGAAAGCTAGTAGAAGCCGGGCAGCTAGAGTTTGTAAATGGTGGTTGGTGCATGCATGATGAAGCAACATGCCATTACATAGACATGATTGACCAGACGACACTAGGTCACCATGCAATCAAGGAGCAATTCAACAAGGCCCCCCGAGCAGGATGGCAAATCGATACATTTGGACACTCTGCTGTGCAGGCTTACTTGCTCGGAGCTGAGCTTGGATTTGATTCTGTACATTTTGCAAGGATTGATTATCAGGACAGAGAAAAGCGAAAGAGAGATAAGTCTCTTGAAGTTATATGGCGTGGATCCAGAACGCTTGGTTCTTCATCTCAGATTTTTGCCAATGCCTTTCCTGTTCACTACGGTCCACCCCCAGGTTTCCGTTTTGAAATCAATGGTGACTTCATCCCTATTCAGGATAATCCTTTACTTTTTGACTCCAATGTTGAAAAGCGTGTTCATGATTTTATTAATGCTGCAGTCATACAGGCTAATGTGACAAGGACAAATCACATTATGTGGACAATGGGTGGTGATTTCGAGTACCAATATGCTGAGTCTTGGTTCAAAGAAATGGACAAATTAATTCACTATGTTAATAAGGACGGTCGGGTTAATGCATTGTACTCTACCCCATCAATATATTCTGATGCAAAAAATGCAGCAAATCAGTCATGGCCACTGAAAACCGATGATTATTTTCCTTATGCAGATGTGGTCAACTCTTATTGGACTGGCTTTTTTACTAGTCGCCCCAGTCTGAAGCGATATGTAAGAATGTTGAGTGGCTACTACTTGGCAGCAAGGCAACTAGAATTTCTGGTGGGAAAAAGGTCAACTGGTCCAAATACTTTTAGCCTTGGAGATGCTTTAGGAATTGCGCAGCACCACGATGCAGTCACAGGCACTTCTAAACAGCACACAACAAGTGACTATGCAAAGCGCCTGTCTATTGGTGCCTCTGAGGCAGAAGCTGTTGTCAGTGCTGCTTTATCATGCCTAACTACAGGTAAATCAGGAGAGCAATGTAAGGACCCGGCATTGACTTTTAGCCAGTGTCAATTACTCAATATCAGTTATTGCCCTCCAACCGAGGAAGGGATACCAGAAGGAAAGAGTTTGGTTGTAGTGGCTTATAACCCTCTTGGATGGAATAGAACTGAGAATGTTAGAATACCTGTTAATGATGCCAATCTTGCTGTCCAAGATTCCCTCGGTAATTCTCTTGAGGCACAGTTTGTAAGTTTGGATAATGTTACAATAAATATAAGAAATTTCTATACAAAAGCATACTTGGGACTGTCATCAAAACAAGTTCCCAAGTTCTGGCTGATCTTTCAAGTCTCAGTGCCACCACTTGGTTGGAACACATACTTCATTTCTAGACGAGCTACCAGAGGGAAAAACAGAAATGGATTTTTCTCTGTGATGGATAGTCCACAGAATGAGACCATTGAAATTGGACCTGGAGACCTGAAGATGTCATTTTCTGTTGCTTCTGGACAACTCCAACGGATGTATGATTCCAAAACAGGAGTTGATGTACCAATTCAGCAGAGCTTCCTTTGGTATAATTCAAGCTCAGAAATTCCAGGTTCTGGTGCATACATTTTTCGCCCCGAGTCCTTTCCAAAAATTGTGTCAAGATCAGTAAACTTGAAGGTCTTTCGTGGACCACTAGTCGATGAGATTCACCAAGAGTTCAGTTCATGGATCTATCAGGTCACTAGACTTTACAGGGACAAACAGCACGCGGAAGTTGAGTTCACTATCGGTCCAATTCCCACCGAAGATTATACTGGAAGAGAGGTCATCACTAGAATGACAACTAATATGACCACAGAGAAGGTGTTCTACACTGATTCAAATGGAAGGGATTTCATAAAACGGGTTCGAGATTATAGATCCGACTGGTCCCTTTTGGTTAACCAACCTGTAGCAGGAAATTATTATCCATTAAATCTTGGCATATTTACCTCGGATAAGAAATATGAGTTTTCGGTCCTGGTAGATCGTGCCACTGGAGGAGCTAGCATTAAAGATGGAGAACTGGAGCTCATGTTGGGAAAAGTAATAGAATGGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009791 GO:0009889 GO:0009908 GO:0009954 GO:0009965 GO:0010016 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048441 GO:0048465 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0099402 GO:1903506 GO:1905392 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LOB

Protein Analysis

817

Amino Acids

92.24

Weight (kDa)

6.46

Isoelectric Point (pI)

38.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	48 - 359	8.5e-79	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	364 - 456	1.5e-18	Alpha mannosidase middle domain
Laman-like_dom	PF21260	533 - 582	3.4e-07	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	612 - 814	1.9e-42	Glycosyl hydrolases family 38 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008830)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g15510 FvH4_1g15510
malus_domestica	MD02G1166900.v1.1 MD15G1279500.v1.1
prunus_persica	Prupe.7G137900_v2.0.a1 Prupe.7G137900_v2.0.a1
pyrus_communis	pycom02g13420 pycom15g24240
rosa_chinensis	RchiOBHm_Chr2g0104791
rosa_laevigata	RLG00000017356
rosa_multiflora	Rmu_co7961052.1_g000001
rosa_roxburghii	Rroxscaffold_2G00138780
rosa_rugosa	Rorug02G0122600 Rorug02G0122700 Rorug02G0122800 Rorug02G0122900
rosa_samantha	Rh2AG172700 Rh2BG180400 Rh2CG178000 Rh2DG178900
rosa_wichuraiana	Rw0G008660 Rw2G013500 Rw2G013580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1503
Acc16I	TGCGCA	1 cut(s)	1248
AccB1I	GGYRCC	1 cut(s)	1322
AccB7I	CCANNNNNTGG	3 cut(s)	1692, 1724, 1964
AccII	CGCG	1 cut(s)	2120
AciI	CCGC	2 cut(s)	93, 2120
AclWI	GGATC	6 cut(s)	220, 278, 666, 679, 2087, 2265
AcoI	YGGCCR	1 cut(s)	1043
AcsI	RAATTY	4 cut(s)	296, 1181, 1639, 1953
AcuI	CTGAAG	2 cut(s)	1148, 1862
AfaI	GTAC	6 cut(s)	107, 203, 597, 911, 992, 1911
AfiI	CCNNNNNNNGG	8 cut(s)	518, 740, 781, 1464, 1692, 1724, 1964, 2306
AhdI	GACNNNNNGTC	2 cut(s)	474, 2045
AhlI	ACTAGT	2 cut(s)	1112, 2044
AjnI	CCWGG	4 cut(s)	739, 1831, 1957, 2370
AloI	GAACNNNNNNTCC	2 cut(s)	2114, 2146
Alw21I	GWGCWC	1 cut(s)	2424
Alw26I	GTCTC	4 cut(s)	654, 1714, 1808, 1830
AlwI	GGATC	6 cut(s)	220, 278, 666, 679, 2087, 2265
AlwNI	CAGNNNCTG	2 cut(s)	1340, 1964
Ama87I	CYCGRG	2 cut(s)	513, 1985
AoxI	GGCC	3 cut(s)	18, 507, 1043
ApeKI	GCWGC	7 cut(s)	233, 391, 842, 1028, 1166, 1249, 1352
ApoI	RAATTY	4 cut(s)	296, 1181, 1639, 1953
ArsI	GACNNNNNNTTYG	4 cut(s)	1298, 1330, 1991, 2023
AseI	ATTAAT	2 cut(s)	837, 950
Asp700I	GAANNNNTTC	1 cut(s)	2216
AspLEI	GCGC	2 cut(s)	1249, 1311
AspS9I	GGNCC	9 cut(s)	508, 731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
AsuC2I	CCSGG	2 cut(s)	388, 1403
AsuHPI	GGTGA	4 cut(s)	470, 776, 911, 2051
AsuNHI	GCTAGC	1 cut(s)	2396
AvaI	CYCGRG	2 cut(s)	513, 1985
AvaII	GGWCC	8 cut(s)	731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
BalI	TGGCCA	1 cut(s)	1045
BamHI	GGATCC	1 cut(s)	671
BanI	GGYRCC	1 cut(s)	1322
BanII	GRGCYC	1 cut(s)	2424
BarI	GAAGNNNNNNTAC	2 cut(s)	2204, 2236
BbsI	GAAGAC	1 cut(s)	194
Bbv12I	GWGCWC	1 cut(s)	2424
BbvCI	CCTCAGC	1 cut(s)	22
BbvI	GCAGC	7 cut(s)	220, 403, 829, 1040, 1178, 1261, 1339
BccI	CCATC	8 cut(s)	174, 307, 324, 516, 1009, 1509, 1789, 2402
BciT130I	CCWGG	4 cut(s)	741, 1833, 1959, 2372
BciVI	GTATCC	1 cut(s)	1461
BclI	TGATCA	1 cut(s)	196
BcnI	CCSGG	2 cut(s)	388, 1403
BcoDI	GTCTC	4 cut(s)	654, 1714, 1808, 1830
BcuI	ACTAGT	2 cut(s)	1112, 2044
BfmI	CTRYAG	3 cut(s)	843, 1371, 2301
BfoI	RGCGCY	1 cut(s)	1312
BfuI	GTATCC	1 cut(s)	1461
BglI	GCCNNNNNGGC	1 cut(s)	1331
BisI	GCNGC	7 cut(s)	234, 392, 843, 1029, 1167, 1250, 1353
BlpI	GCTNAGC	1 cut(s)	576
BlsI	GCNGC	7 cut(s)	235, 393, 844, 1030, 1168, 1251, 1354
BmcAI	AGTACT	1 cut(s)	203
Bme1390I	CCNGG	6 cut(s)	388, 741, 1403, 1833, 1959, 2372
Bme18I	GGWCC	8 cut(s)	731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
BmeRI	GACNNNNNGTC	2 cut(s)	474, 2045
BmeT110I	CYCGRG	2 cut(s)	513, 1985
BmgT120I	GGNCC	9 cut(s)	508, 731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
BmiI	GGNNCC	5 cut(s)	510, 673, 1324, 1401, 2282
BmrFI	CCNGG	6 cut(s)	388, 741, 1403, 1833, 1959, 2372
BmrI	ACTGGG	1 cut(s)	1116
BmsI	GCATC	4 cut(s)	1006, 1222, 1249, 1543
BmtI	GCTAGC	1 cut(s)	2400
BmuI	ACTGGG	1 cut(s)	1116
BpiI	GAAGAC	1 cut(s)	194
BpmI	CTGGAG	3 cut(s)	1854, 2409, 2438
Bpu10I	CCTNAGC	1 cut(s)	22
Bpu1102I	GCTNAGC	1 cut(s)	576
BpuEI	CTTGAG	1 cut(s)	1613
BpuMI	CCSGG	2 cut(s)	388, 1403
Bsa29I	ATCGAT	1 cut(s)	531
BsaBI	GATNNNNATC	1 cut(s)	1557
BsaI	GGTCTC	2 cut(s)	1808, 1830
BsaJI	CCNNGG	5 cut(s)	739, 1225, 1458, 1579, 2343
BsaXI	ACNNNNNCTCC	4 cut(s)	1893, 1923, 2411, 2441
Bsc4I	CCNNNNNNNGG	8 cut(s)	518, 740, 781, 1464, 1692, 1724, 1964, 2306
Bse1I	ACTGG	8 cut(s)	1105, 1122, 1210, 1420, 2170, 2282, 2392, 2421
Bse3DI	GCAATG	1 cut(s)	1397
Bse8I	GATNNNNATC	1 cut(s)	1557
BseBI	CCWGG	4 cut(s)	741, 1833, 1959, 2372
BseCI	ATCGAT	1 cut(s)	531
BseDI	CCNNGG	5 cut(s)	739, 1225, 1458, 1579, 2343
BseGI	GGATG	5 cut(s)	185, 527, 771, 1520, 1887
BseJI	GATNNNNATC	1 cut(s)	1557
BseLI	CCNNNNNNNGG	8 cut(s)	518, 740, 781, 1464, 1692, 1724, 1964, 2306
BseMI	GCAATG	1 cut(s)	1397
BseMII	CTCAG	9 cut(s)	36, 300, 567, 710, 912, 1320, 1518, 1725, 1962
BseNI	ACTGG	8 cut(s)	1105, 1122, 1210, 1420, 2170, 2282, 2392, 2421
BseRI	GAGGAG	1 cut(s)	2406
BseXI	GCAGC	7 cut(s)	220, 403, 829, 1040, 1178, 1261, 1339
BsgI	GTGCAG	1 cut(s)	575
Bsh1236I	CGCG	1 cut(s)	2120
Bsh1285I	CGRYCG	1 cut(s)	976
BshFI	GGCC	3 cut(s)	20, 509, 1045
BshNI	GGYRCC	1 cut(s)	1322
BshVI	ATCGAT	1 cut(s)	531
BsiEI	CGRYCG	1 cut(s)	976
BsiHKAI	GWGCWC	1 cut(s)	2424
BsiHKCI	CYCGRG	2 cut(s)	513, 1985
BsiSI	CCGG	2 cut(s)	387, 1403
BslFI	GGGAC	3 cut(s)	1677, 2120, 2266
BslI	CCNNNNNNNGG	8 cut(s)	518, 740, 781, 1464, 1692, 1724, 1964, 2306
BsmAI	GTCTC	4 cut(s)	654, 1714, 1808, 1830
BsmFI	GGGAC	3 cut(s)	1677, 2120, 2266
BsmI	GAATGC	1 cut(s)	225
BsnI	GGCC	3 cut(s)	20, 509, 1045
Bso31I	GGTCTC	2 cut(s)	1808, 1830
BsoBI	CYCGRG	2 cut(s)	513, 1985
Bsp1286I	GDGCHC	1 cut(s)	2424
Bsp1407I	TGTACA	1 cut(s)	595
Bsp143I	GATC	9 cut(s)	196, 212, 283, 671, 1698, 2012, 2079, 2270, 2377
Bsp1720I	GCTNAGC	1 cut(s)	576
BspACI	CCGC	2 cut(s)	93, 2120
BspANI	GGCC	3 cut(s)	20, 509, 1045
BspCNI	CTCAG	9 cut(s)	35, 301, 568, 709, 913, 1321, 1519, 1724, 1961
BspDI	ATCGAT	1 cut(s)	531
BspFNI	CGCG	1 cut(s)	2120
BspHI	TCATGA	1 cut(s)	826
BspLI	GGNNCC	5 cut(s)	510, 673, 1324, 1401, 2282
BspMAI	CTGCAG	1 cut(s)	847
BspOI	GCTAGC	1 cut(s)	2400
BspPI	GGATC	6 cut(s)	220, 278, 666, 679, 2087, 2265
BspT107I	GGYRCC	1 cut(s)	1322
BspTNI	GGTCTC	2 cut(s)	1808, 1830
BsrDI	GCAATG	1 cut(s)	1397
BsrGI	TGTACA	1 cut(s)	595
BsrI	ACTGG	8 cut(s)	1105, 1122, 1210, 1420, 2170, 2282, 2392, 2421
BssECI	CCNNGG	5 cut(s)	739, 1225, 1458, 1579, 2343
BssMI	GATC	9 cut(s)	196, 212, 283, 671, 1698, 2012, 2079, 2270, 2377
BssT1I	CCWWGG	1 cut(s)	1225
Bst2UI	CCWGG	4 cut(s)	741, 1833, 1959, 2372
Bst4CI	ACNGT	6 cut(s)	193, 349, 731, 974, 1602, 1668
Bst6I	CTCTTC	1 cut(s)	2164
BstAPI	GCANNNNNTGC	1 cut(s)	1025
BstAUI	TGTACA	1 cut(s)	595
BstC8I	GCNNGC	4 cut(s)	422, 558, 2118, 2398
BstDEI	CTNAG	9 cut(s)	22, 309, 576, 696, 921, 1329, 1527, 1711, 1948
BstEII	GGTNACC	1 cut(s)	476
BstENI	CCTNNNNNAGG	1 cut(s)	2304
BstF5I	GGATG	5 cut(s)	185, 527, 771, 1520, 1887
BstFNI	CGCG	1 cut(s)	2120
BstH2I	RGCGCY	1 cut(s)	1312
BstHHI	GCGC	2 cut(s)	1249, 1311
BstKTI	GATC	9 cut(s)	199, 215, 286, 674, 1701, 2015, 2082, 2273, 2380
BstMAI	GTCTC	4 cut(s)	654, 1714, 1808, 1830
BstMBI	GATC	9 cut(s)	196, 212, 283, 671, 1698, 2012, 2079, 2270, 2377
BstMCI	CGRYCG	1 cut(s)	976
BstMWI	GCNNNNNNNGC	6 cut(s)	314, 515, 557, 1025, 1331, 1562
BstNI	CCWGG	4 cut(s)	741, 1833, 1959, 2372
BstNSI	RCATGY	3 cut(s)	240, 424, 441
BstPI	GGTNACC	1 cut(s)	476
BstSCI	CCNGG	6 cut(s)	386, 739, 1401, 1831, 1957, 2370
BstSFI	CTRYAG	3 cut(s)	843, 1371, 2301
BstUI	CGCG	1 cut(s)	2120
BstV1I	GCAGC	7 cut(s)	220, 403, 829, 1040, 1178, 1261, 1339
BstV2I	GAAGAC	1 cut(s)	194
BstX2I	RGATCY	3 cut(s)	671, 2079, 2270
BstXI	CCANNNNNNTGG	1 cut(s)	1728
BstYI	RGATCY	3 cut(s)	671, 2079, 2270
Bsu15I	ATCGAT	1 cut(s)	531
BsuI	GTATCC	1 cut(s)	1461
BsuRI	GGCC	3 cut(s)	20, 509, 1045
BsuTUI	ATCGAT	1 cut(s)	531
BtsCI	GGATG	5 cut(s)	185, 527, 771, 1520, 1887
BtsI	GCAGTG	1 cut(s)	19
BtsIMutI	CAGTG	7 cut(s)	19, 1046, 1354, 1427, 1719, 2223, 2385
Cac8I	GCNNGC	4 cut(s)	422, 558, 2118, 2398
CaiI	CAGNNNCTG	2 cut(s)	1340, 1964
CciI	TCATGA	1 cut(s)	826
CfoI	GCGC	2 cut(s)	1249, 1311
Cfr13I	GGNCC	9 cut(s)	508, 731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
ClaI	ATCGAT	1 cut(s)	531
Csp6I	GTAC	6 cut(s)	106, 202, 596, 910, 991, 1910
CspCI	CAANNNNNGTGG	2 cut(s)	314, 349
CviQI	GTAC	6 cut(s)	106, 202, 596, 910, 991, 1910
DdeI	CTNAG	9 cut(s)	22, 309, 576, 696, 921, 1329, 1527, 1711, 1948
DpnI	GATC	9 cut(s)	198, 214, 285, 673, 1700, 2014, 2081, 2272, 2379
DpnII	GATC	9 cut(s)	196, 212, 283, 671, 1698, 2012, 2079, 2270, 2377
DriI	GACNNNNNGTC	2 cut(s)	474, 2045
EaeI	YGGCCR	1 cut(s)	1043
Eam1104I	CTCTTC	1 cut(s)	2164
Eam1105I	GACNNNNNGTC	2 cut(s)	474, 2045
EarI	CTCTTC	1 cut(s)	2164
Ecl136II	GAGCTC	1 cut(s)	2422
Eco130I	CCWWGG	1 cut(s)	1225
Eco24I	GRGCYC	1 cut(s)	2424
Eco31I	GGTCTC	2 cut(s)	1808, 1830
Eco47I	GGWCC	8 cut(s)	731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
Eco53kI	GAGCTC	1 cut(s)	2422
Eco57I	CTGAAG	2 cut(s)	1148, 1862
Eco88I	CYCGRG	2 cut(s)	513, 1985
Eco91I	GGTNACC	1 cut(s)	476
EcoICRI	GAGCTC	1 cut(s)	2422
EcoNI	CCTNNNNNAGG	1 cut(s)	2304
EcoO109I	RGGNCCY	2 cut(s)	508, 1399
EcoO65I	GGTNACC	1 cut(s)	476
EcoRII	CCWGG	4 cut(s)	739, 1831, 1957, 2370
EcoT14I	CCWWGG	1 cut(s)	1225
EcoT22I	ATGCAT	2 cut(s)	426, 988
EcoT38I	GRGCYC	1 cut(s)	2424
ErhI	CCWWGG	1 cut(s)	1225
FaqI	GGGAC	3 cut(s)	1677, 2120, 2266
FbaI	TGATCA	1 cut(s)	196
Fnu4HI	GCNGC	7 cut(s)	234, 392, 843, 1029, 1167, 1250, 1353
FokI	GGATG	5 cut(s)	192, 534, 758, 1527, 1894
FriOI	GRGCYC	1 cut(s)	2424
Fsp4HI	GCNGC	7 cut(s)	234, 392, 843, 1029, 1167, 1250, 1353
FspI	TGCGCA	1 cut(s)	1248
GlaI	GCGC	2 cut(s)	1248, 1310
GluI	GCNGC	7 cut(s)	234, 392, 843, 1029, 1167, 1250, 1353
GsuI	CTGGAG	3 cut(s)	1854, 2409, 2438
HaeII	RGCGCY	1 cut(s)	1312
HaeIII	GGCC	3 cut(s)	20, 509, 1045
HapII	CCGG	2 cut(s)	387, 1403
HhaI	GCGC	2 cut(s)	1249, 1311
Hin6I	GCGC	2 cut(s)	1247, 1309
HinP1I	GCGC	2 cut(s)	1247, 1309
HincII	GTYRAC	4 cut(s)	339, 1087, 1203, 2293
HindII	GTYRAC	4 cut(s)	339, 1087, 1203, 2293
HpaI	GTTAAC	2 cut(s)	339, 2293
HpaII	CCGG	2 cut(s)	387, 1403
HphI	GGTGA	4 cut(s)	470, 776, 911, 2051
Hpy188I	TCNGA	9 cut(s)	288, 572, 699, 1015, 1128, 1330, 1951, 2275, 2347
Hpy99I	CGWCG	1 cut(s)	176
HpyAV	CCTTC	6 cut(s)	125, 1457, 1469, 2020, 2206, 2232
HpyCH4III	ACNGT	6 cut(s)	193, 349, 731, 974, 1602, 1668
HpyCH4IV	ACGT	1 cut(s)	174
HpyF10VI	GCNNNNNNNGC	6 cut(s)	314, 515, 557, 1025, 1331, 1562
HpyF3I	CTNAG	9 cut(s)	22, 309, 576, 696, 921, 1329, 1527, 1711, 1948
HpySE526I	ACGT	1 cut(s)	174
HspAI	GCGC	2 cut(s)	1247, 1309
Ksp22I	TGATCA	1 cut(s)	196
KspAI	GTTAAC	2 cut(s)	339, 2293
Kzo9I	GATC	9 cut(s)	196, 212, 283, 671, 1698, 2012, 2079, 2270, 2377
LmnI	GCTCC	4 cut(s)	493, 572, 2393, 2419
Lsp1109I	GCAGC	7 cut(s)	220, 403, 829, 1040, 1178, 1261, 1339
LweI	GCATC	4 cut(s)	1006, 1222, 1249, 1543
MaeII	ACGT	1 cut(s)	174
MaeIII	GTNAC	8 cut(s)	74, 476, 764, 862, 1264, 1295, 1621, 2089
MalI	GATC	9 cut(s)	198, 214, 285, 673, 1700, 2014, 2081, 2272, 2379
MboI	GATC	9 cut(s)	196, 212, 283, 671, 1698, 2012, 2079, 2270, 2377
MboII	GAAGA	5 cut(s)	199, 681, 1855, 2168, 2181
MflI	RGATCY	3 cut(s)	671, 2079, 2270
MhlI	GDGCHC	1 cut(s)	2424
MlsI	TGGCCA	1 cut(s)	1045
MluNI	TGGCCA	1 cut(s)	1045
MlyI	GAGTC	4 cut(s)	251, 797, 932, 1997
MmeI	TCCRAC	7 cut(s)	157, 190, 234, 1478, 1709, 1900, 2298
Mox20I	TGGCCA	1 cut(s)	1045
Mph1103I	ATGCAT	2 cut(s)	426, 988
MroXI	GAANNNNTTC	1 cut(s)	2216
MscI	TGGCCA	1 cut(s)	1045
MslI	CAYNNNNRTG	4 cut(s)	165, 452, 1257, 2186
Msp20I	TGGCCA	1 cut(s)	1045
MspI	CCGG	2 cut(s)	387, 1403
MspR9I	CCNGG	6 cut(s)	388, 741, 1403, 1833, 1959, 2372
Mva1269I	GAATGC	1 cut(s)	225
MvaI	CCWGG	4 cut(s)	741, 1833, 1959, 2372
MvnI	CGCG	1 cut(s)	2120
MwoI	GCNNNNNNNGC	6 cut(s)	314, 515, 557, 1025, 1331, 1562
NciI	CCSGG	2 cut(s)	388, 1403
NdeII	GATC	9 cut(s)	196, 212, 283, 671, 1698, 2012, 2079, 2270, 2377
NheI	GCTAGC	1 cut(s)	2396
NlaIV	GGNNCC	5 cut(s)	510, 673, 1324, 1401, 2282
NmuCI	GTSAC	6 cut(s)	476, 764, 862, 1264, 1295, 2089
NsbI	TGCGCA	1 cut(s)	1248
NsiI	ATGCAT	2 cut(s)	426, 988
NspI	RCATGY	3 cut(s)	240, 424, 441
PaeI	GCATGC	1 cut(s)	424
PagI	TCATGA	1 cut(s)	826
PctI	GAATGC	1 cut(s)	225
PdmI	GAANNNNTTC	1 cut(s)	2216
PfeI	GAWTC	6 cut(s)	269, 590, 1574, 1889, 2056, 2228
PflFI	GACNNNGTC	1 cut(s)	260
PflMI	CCANNNNNTGG	3 cut(s)	1692, 1724, 1964
PkrI	GCNGC	7 cut(s)	235, 393, 844, 1030, 1168, 1251, 1354
PleI	GAGTC	4 cut(s)	251, 797, 931, 1996
PpsI	GAGTC	4 cut(s)	251, 797, 931, 1996
PpuMI	RGGWCCY	1 cut(s)	1399
PshBI	ATTAAT	2 cut(s)	837, 950
PsiI	TTATAA	1 cut(s)	1503
Psp124BI	GAGCTC	1 cut(s)	2424
Psp5II	RGGWCCY	1 cut(s)	1399
Psp6I	CCWGG	4 cut(s)	739, 1831, 1957, 2370
PspEI	GGTNACC	1 cut(s)	476
PspGI	CCWGG	4 cut(s)	739, 1831, 1957, 2370
PspN4I	GGNNCC	5 cut(s)	510, 673, 1324, 1401, 2282
PspPI	GGNCC	9 cut(s)	508, 731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
PspPPI	RGGWCCY	1 cut(s)	1399
PstI	CTGCAG	1 cut(s)	847
PstNI	CAGNNNCTG	2 cut(s)	1340, 1964
PsuI	RGATCY	3 cut(s)	671, 2079, 2270
PsyI	GACNNNGTC	1 cut(s)	260
RsaI	GTAC	6 cut(s)	107, 203, 597, 911, 992, 1911
RsaNI	GTAC	6 cut(s)	106, 202, 596, 910, 991, 1910
RseI	CAYNNNNRTG	4 cut(s)	165, 452, 1257, 2186
SacI	GAGCTC	1 cut(s)	2424
SatI	GCNGC	7 cut(s)	234, 392, 843, 1029, 1167, 1250, 1353
Sau3AI	GATC	9 cut(s)	196, 212, 283, 671, 1698, 2012, 2079, 2270, 2377
Sau96I	GGNCC	9 cut(s)	508, 731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
ScaI	AGTACT	1 cut(s)	203
SchI	GAGTC	4 cut(s)	251, 797, 932, 1997
ScrFI	CCNGG	6 cut(s)	388, 741, 1403, 1833, 1959, 2372
SduI	GDGCHC	1 cut(s)	2424
SfaNI	GCATC	4 cut(s)	1006, 1222, 1249, 1543
SfcI	CTRYAG	3 cut(s)	843, 1371, 2301
SinI	GGWCC	8 cut(s)	731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
SmiMI	CAYNNNNRTG	4 cut(s)	165, 452, 1257, 2186
SmlI	CTYRAG	1 cut(s)	1592
SmoI	CTYRAG	1 cut(s)	1592
SpeI	ACTAGT	2 cut(s)	1112, 2044
SphI	GCATGC	1 cut(s)	424
SsiI	CCGC	2 cut(s)	93, 2120
SstI	GAGCTC	1 cut(s)	2424
StyD4I	CCNGG	6 cut(s)	386, 739, 1401, 1831, 1957, 2370
StyI	CCWWGG	1 cut(s)	1225
TaaI	ACNGT	6 cut(s)	193, 349, 731, 974, 1602, 1668
TaiI	ACGT	1 cut(s)	177
TaqI	TCGA	4 cut(s)	531, 906, 2049, 2260
TaqII	GACCGA	2 cut(s)	2129, 2356
TatI	WGTACW	4 cut(s)	105, 201, 595, 990
TfiI	GAWTC	6 cut(s)	269, 590, 1574, 1889, 2056, 2228
TscAI	CASTG	7 cut(s)	19, 1053, 1354, 1427, 1719, 2230, 2392
TseFI	GTSAC	6 cut(s)	476, 764, 862, 1264, 1295, 2089
TseI	GCWGC	7 cut(s)	233, 391, 842, 1028, 1166, 1249, 1352
Tsp45I	GTSAC	6 cut(s)	476, 764, 862, 1264, 1295, 2089
TspDTI	ATGAA	9 cut(s)	20, 137, 444, 681, 760, 815, 1732, 2064, 2236
TspGWI	ACGGA	2 cut(s)	737, 1894
TspRI	CASTG	7 cut(s)	19, 1053, 1354, 1427, 1719, 2230, 2392
Tth111I	GACNNNGTC	1 cut(s)	260
Van91I	CCANNNNNTGG	3 cut(s)	1692, 1724, 1964
VpaK11BI	GGWCC	8 cut(s)	731, 1208, 1399, 1829, 2039, 2141, 2280, 2368
VspI	ATTAAT	2 cut(s)	837, 950
XagI	CCTNNNNNAGG	1 cut(s)	2304
XapI	RAATTY	4 cut(s)	296, 1181, 1639, 1953
XbaI	TCTAGA	1 cut(s)	1748
XceI	RCATGY	3 cut(s)	240, 424, 441
XcmI	CCANNNNNNNNNTGG	2 cut(s)	173, 1725
XmnI	GAANNNNTTC	1 cut(s)	2216
ZrmI	AGTACT	1 cut(s)	203
Zsp2I	ATGCAT	2 cut(s)	426, 988

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.