pycom02g13420

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008830
Physical Location & Seq
Reverse (-)
9941658 .. 9942572
915 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13420.1

Sequence Viewer

Length: 915 bp
ATGTCGTCGTCCTCAATGTCGTCGTCTTCAAACTCTCCGTGCGCAGCGTGCAAATTTCTACGGCGGAAGTGCACGCCGGAGTGCGTCTTCGCGCCTTACTTCCCACCTGACAACCCCCAAAGGTTTGCCAACGTCCACAAGGTCTTCGGCGCCAGCAATGTCGCCAAGATCCTCAACGAGCTCAGCGCTTCTCAGCGCGATGACGCCGTCAACTCCCTGGCCTACGAGGCGGAGGCCCGCCTCCGGGACCCTGTCTATGGCTGCGTCGGACTCATCTCCCTCCTCCAGCAGCAGCTGAAGCAAGTCCAGGCGGACCTTTTCAATGCCAAAAAGGAGCTCGCAACTTACATAGGCCCACAAGCTATGCTCTCCATTCTCCAGCCGCCGACTTACATGGCTCAGCAGCTCCAACGAAACTCTTCTTCCTCGGCCGGTCCGCCGTACAGCATGTCTCCGATGATGGGCATTCCGACTGCGGCTGCTTCCGGTCAGCTGATGGTTCGAGATCCCCAGCAGCAGCAACAGCATCAACAGATTTTCGAGGCGCAGCAGTTGGCGGCGGCCGTGGCGGCGAGGGAGCAGCAGGAGATGTTCAGGAGTTTTGATCAGAGTAAACAGGCAATGCAGCAGCAGGCGCAGGACCATTTGAGCGGGTTCAGTCAGATGGCGGGAGCGGTGGGCAACGCTGTTTTGCCGTCTTTGGCTCTTGGGTTTGATAGCGGTCCGTACCACCAGATTCAGCCGCAGCACCCTCACCATAATCACCGCCACCAGCTGCAGCTTCAGGCTCAGCTGTTGCTCCAAACCCAACAGCCGCAAGGGCACCATCAGGCTCAGCAATCCCAGCAACCCCAGCTGCAGGCAAAACCCGTTCCGGGCGAGGAAGGTGGTGGTAGGAGTGTGGGTCCCTCTTGA

Protein Analysis

305

Amino Acids

33.19

Weight (kDa)

8.28

Isoelectric Point (pI)

75.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 13 - 110 3.4e-42 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 43
AccB1I GGYRCC 2 cut(s) 149, 822
AccBSI CCGCTC 2 cut(s) 651, 674
AccII CGCG 2 cut(s) 92, 198
AclWI GGATC 2 cut(s) 163, 500
AcoI YGGCCR 2 cut(s) 429, 561
AcsI RAATTY 1 cut(s) 53
AcuI CTGAAG 2 cut(s) 317, 767
AcyI GRCGYC 2 cut(s) 150, 204
AfaI GTAC 2 cut(s) 443, 728
AfeI AGCGCT 1 cut(s) 187
AfiI CCNNNNNNNGG 6 cut(s) 243, 244, 257, 461, 859, 875
AgsI TTSAA 2 cut(s) 30, 322
AjnI CCWGG 2 cut(s) 216, 306
Alw21I GWGCWC 3 cut(s) 74, 183, 339
Alw26I GTCTC 1 cut(s) 456
Alw44I GTGCAC 1 cut(s) 70
AlwI GGATC 2 cut(s) 163, 500
AlwNI CAGNNNCTG 1 cut(s) 295
Aor51HI AGCGCT 1 cut(s) 187
AoxI GGCC 5 cut(s) 219, 234, 352, 429, 561
ApaLI GTGCAC 1 cut(s) 70
ApoI RAATTY 1 cut(s) 53
Asp700I GAANNNNTTC 1 cut(s) 418
AspLEI GCGC 7 cut(s) 44, 94, 152, 188, 198, 547, 637
AspS9I GGNCC 8 cut(s) 235, 247, 313, 353, 434, 640, 722, 905
AsuC2I CCSGG 2 cut(s) 245, 876
AsuHPI GGTGA 2 cut(s) 746, 755
AvaII GGWCC 6 cut(s) 247, 313, 434, 640, 722, 905
BaeGI GKGCMC 2 cut(s) 74, 825
BanI GGYRCC 2 cut(s) 149, 822
BanII GRGCYC 2 cut(s) 183, 339
BbsI GAAGAC 3 cut(s) 18, 79, 136
Bbv12I GWGCWC 3 cut(s) 74, 183, 339
BccI CCATC 4 cut(s) 454, 490, 658, 834
BceAI ACGGC 5 cut(s) 77, 191, 424, 548, 679
BciT130I CCWGG 2 cut(s) 218, 308
BclI TGATCA 1 cut(s) 604
BcnI CCSGG 2 cut(s) 245, 876
BcoDI GTCTC 1 cut(s) 456
BfmI CTRYAG 2 cut(s) 776, 857
BfoI RGCGCY 2 cut(s) 153, 189
BglI GCCNNNNNGGC 3 cut(s) 227, 569, 820
BlpI GCTNAGC 4 cut(s) 182, 399, 789, 834
Bme1390I CCNGG 4 cut(s) 218, 245, 308, 876
Bme18I GGWCC 6 cut(s) 247, 313, 434, 640, 722, 905
BmgT120I GGNCC 8 cut(s) 235, 247, 313, 353, 434, 640, 722, 905
BmiI GGNNCC 6 cut(s) 151, 248, 249, 824, 906, 907
BmrFI CCNGG 4 cut(s) 218, 245, 308, 876
BmsI GCATC 1 cut(s) 535
BpiI GAAGAC 3 cut(s) 18, 79, 136
BpmI CTGGAG 2 cut(s) 269, 362
Bpu1102I GCTNAGC 4 cut(s) 182, 399, 789, 834
BpuMI CCSGG 2 cut(s) 245, 876
BsaHI GRCGYC 2 cut(s) 150, 204
BsaJI CCNNGG 3 cut(s) 216, 426, 564
BsaWI WCCGGW 1 cut(s) 485
BsaXI ACNNNNNCTCC 1 cut(s) 889
Bsc4I CCNNNNNNNGG 6 cut(s) 243, 244, 257, 461, 859, 875
Bse118I RCCGGY 1 cut(s) 431
Bse3DI GCAATG 2 cut(s) 163, 627
BseBI CCWGG 2 cut(s) 218, 308
BseDI CCNNGG 3 cut(s) 216, 426, 564
BseLI CCNNNNNNNGG 6 cut(s) 243, 244, 257, 461, 859, 875
BseMI GCAATG 2 cut(s) 163, 627
BseMII CTCAG 5 cut(s) 196, 206, 413, 803, 848
BseRI GAGGAG 1 cut(s) 272
BseSI GKGCMC 2 cut(s) 74, 825
BseX3I CGGCCG 2 cut(s) 429, 561
BseYI CCCAGC 3 cut(s) 510, 843, 852
Bsh1236I CGCG 2 cut(s) 92, 198
Bsh1285I CGRYCG 2 cut(s) 432, 564
BshFI GGCC 5 cut(s) 221, 236, 354, 431, 563
BshNI GGYRCC 2 cut(s) 149, 822
BsiEI CGRYCG 2 cut(s) 432, 564
BsiHKAI GWGCWC 3 cut(s) 74, 183, 339
BsiSI CCGG 5 cut(s) 77, 244, 432, 486, 875
BslFI GGGAC 2 cut(s) 260, 891
BslI CCNNNNNNNGG 6 cut(s) 243, 244, 257, 461, 859, 875
BsmAI GTCTC 1 cut(s) 456
BsmFI GGGAC 2 cut(s) 260, 891
BsmI GAATGC 1 cut(s) 465
BsnI GGCC 5 cut(s) 221, 236, 354, 431, 563
Bsp1286I GDGCHC 4 cut(s) 74, 183, 339, 825
Bsp143I GATC 3 cut(s) 168, 505, 604
Bsp1720I GCTNAGC 4 cut(s) 182, 399, 789, 834
BspANI GGCC 5 cut(s) 221, 236, 354, 431, 563
BspCNI CTCAG 5 cut(s) 195, 205, 412, 802, 847
BspFNI CGCG 2 cut(s) 92, 198
BspLI GGNNCC 6 cut(s) 151, 248, 249, 824, 906, 907
BspMAI CTGCAG 2 cut(s) 780, 861
BspPI GGATC 2 cut(s) 163, 500
BspT107I GGYRCC 2 cut(s) 149, 822
BsrBI CCGCTC 2 cut(s) 651, 674
BsrDI GCAATG 2 cut(s) 163, 627
BsrFI RCCGGY 1 cut(s) 431
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 3 cut(s) 216, 426, 564
BssMI GATC 3 cut(s) 168, 505, 604
BssNI GRCGYC 2 cut(s) 150, 204
Bst2UI CCWGG 2 cut(s) 218, 308
Bst6I CTCTTC 1 cut(s) 424
BstACI GRCGYC 2 cut(s) 150, 204
BstC8I GCNNGC 7 cut(s) 49, 74, 154, 238, 339, 633, 861
BstDEI CTNAG 5 cut(s) 182, 192, 399, 789, 834
BstDSI CCRYGG 1 cut(s) 564
BstFNI CGCG 2 cut(s) 92, 198
BstH2I RGCGCY 2 cut(s) 153, 189
BstHHI GCGC 7 cut(s) 44, 94, 152, 188, 198, 547, 637
BstKTI GATC 3 cut(s) 171, 508, 607
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 3 cut(s) 168, 505, 604
BstMCI CGRYCG 2 cut(s) 432, 564
BstNI CCWGG 2 cut(s) 218, 308
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 4 cut(s) 216, 243, 306, 874
BstSFI CTRYAG 2 cut(s) 776, 857
BstSLI GKGCMC 2 cut(s) 74, 825
BstUI CGCG 2 cut(s) 92, 198
BstV2I GAAGAC 3 cut(s) 18, 79, 136
BstX2I RGATCY 2 cut(s) 168, 505
BstYI RGATCY 2 cut(s) 168, 505
BstZI CGGCCG 2 cut(s) 429, 561
BsuRI GGCC 5 cut(s) 221, 236, 354, 431, 563
BtgI CCRYGG 1 cut(s) 564
BtgZI GCGATG 1 cut(s) 213
Cac8I GCNNGC 7 cut(s) 49, 74, 154, 238, 339, 633, 861
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 7 cut(s) 44, 94, 152, 188, 198, 547, 637
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 8 cut(s) 235, 247, 313, 353, 434, 640, 722, 905
CpoI CGGWCCG 2 cut(s) 434, 722
CseI GACGC 3 cut(s) 73, 212, 253
Csp6I GTAC 2 cut(s) 442, 727
CspI CGGWCCG 2 cut(s) 434, 722
CviAII CATG 2 cut(s) 394, 448
CviQI GTAC 2 cut(s) 442, 727
DdeI CTNAG 5 cut(s) 182, 192, 399, 789, 834
DinI GGCGCC 1 cut(s) 151
DpnI GATC 3 cut(s) 170, 507, 606
DpnII GATC 3 cut(s) 168, 505, 604
EaeI YGGCCR 2 cut(s) 429, 561
EagI CGGCCG 2 cut(s) 429, 561
Eam1104I CTCTTC 1 cut(s) 424
EarI CTCTTC 1 cut(s) 424
EciI GGCGGA 4 cut(s) 79, 245, 326, 426
Ecl136II GAGCTC 2 cut(s) 181, 337
EclXI CGGCCG 2 cut(s) 429, 561
Eco24I GRGCYC 2 cut(s) 183, 339
Eco47I GGWCC 6 cut(s) 247, 313, 434, 640, 722, 905
Eco47III AGCGCT 1 cut(s) 187
Eco52I CGGCCG 2 cut(s) 429, 561
Eco53kI GAGCTC 2 cut(s) 181, 337
Eco57I CTGAAG 2 cut(s) 317, 767
EcoICRI GAGCTC 2 cut(s) 181, 337
EcoO109I RGGNCCY 2 cut(s) 247, 905
EcoRII CCWGG 2 cut(s) 216, 306
EcoT38I GRGCYC 2 cut(s) 183, 339
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
FaeI CATG 2 cut(s) 397, 451
FaiI YATR 6 cut(s) 258, 350, 365, 395, 449, 759
FaqI GGGAC 2 cut(s) 260, 891
FatI CATG 2 cut(s) 393, 447
FauI CCCGC 3 cut(s) 245, 644, 661
FbaI TGATCA 1 cut(s) 604
FriOI GRGCYC 2 cut(s) 183, 339
FspI TGCGCA 1 cut(s) 43
GlaI GCGC 7 cut(s) 43, 93, 151, 187, 197, 546, 636
GsaI CCCAGC 3 cut(s) 514, 847, 856
GsuI CTGGAG 2 cut(s) 269, 362
HaeII RGCGCY 2 cut(s) 153, 189
HaeIII GGCC 5 cut(s) 221, 236, 354, 431, 563
HapII CCGG 5 cut(s) 77, 244, 432, 486, 875
HgaI GACGC 3 cut(s) 73, 212, 253
HhaI GCGC 7 cut(s) 44, 94, 152, 188, 198, 547, 637
Hin1I GRCGYC 2 cut(s) 150, 204
Hin1II CATG 2 cut(s) 397, 451
Hin6I GCGC 7 cut(s) 42, 92, 150, 186, 196, 545, 635
HinP1I GCGC 7 cut(s) 42, 92, 150, 186, 196, 545, 635
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 2 cut(s) 270, 736
HpaII CCGG 5 cut(s) 77, 244, 432, 486, 875
HphI GGTGA 2 cut(s) 746, 755
Hpy166II GTNNAC 4 cut(s) 72, 136, 211, 614
Hpy188I TCNGA 5 cut(s) 269, 456, 471, 609, 663
Hpy188III TCNNGA 3 cut(s) 503, 595, 912
Hpy8I GTNNAC 4 cut(s) 72, 136, 211, 614
Hpy99I CGWCG 3 cut(s) 10, 25, 269
HpyAV CCTTC 1 cut(s) 878
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 5 cut(s) 51, 72, 625, 778, 859
HpyF3I CTNAG 5 cut(s) 182, 192, 399, 789, 834
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 2 cut(s) 150, 204
Hsp92II CATG 2 cut(s) 397, 451
HspAI GCGC 7 cut(s) 42, 92, 150, 186, 196, 545, 635
KasI GGCGCC 1 cut(s) 149
KflI GGGWCCC 2 cut(s) 247, 905
Ksp22I TGATCA 1 cut(s) 604
Kzo9I GATC 3 cut(s) 168, 505, 604
LmnI GCTCC 5 cut(s) 334, 411, 577, 671, 804
LweI GCATC 1 cut(s) 535
MaeII ACGT 1 cut(s) 132
MalI GATC 3 cut(s) 170, 507, 606
MbiI CCGCTC 2 cut(s) 651, 674
MboI GATC 3 cut(s) 168, 505, 604
MboII GAAGA 5 cut(s) 18, 79, 136, 411, 414
MflI RGATCY 2 cut(s) 168, 505
MhlI GDGCHC 4 cut(s) 74, 183, 339, 825
MluCI AATT 1 cut(s) 53
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 1 cut(s) 264
MmeI TCCRAC 3 cut(s) 247, 433, 494
MroXI GAANNNNTTC 1 cut(s) 418
MspA1I CMGCKG 5 cut(s) 295, 493, 775, 793, 856
MspI CCGG 5 cut(s) 77, 244, 432, 486, 875
MspR9I CCNGG 4 cut(s) 218, 245, 308, 876
Mva1269I GAATGC 1 cut(s) 465
MvaI CCWGG 2 cut(s) 218, 308
MvnI CGCG 2 cut(s) 92, 198
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 2 cut(s) 245, 876
NdeII GATC 3 cut(s) 168, 505, 604
NlaIII CATG 2 cut(s) 397, 451
NlaIV GGNNCC 6 cut(s) 151, 248, 249, 824, 906, 907
NmeAIII GCCGAG 1 cut(s) 407
NsbI TGCGCA 1 cut(s) 43
NspI RCATGY 1 cut(s) 451
PctI GAATGC 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 736
PflFI GACNNNGTC 2 cut(s) 206, 251
PfoI TCCNGGA 1 cut(s) 243
PleI GAGTC 1 cut(s) 264
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 264
PpuMI RGGWCCY 2 cut(s) 247, 905
Psp124BI GAGCTC 2 cut(s) 183, 339
Psp5II RGGWCCY 2 cut(s) 247, 905
Psp6I CCWGG 2 cut(s) 216, 306
PspFI CCCAGC 3 cut(s) 510, 843, 852
PspGI CCWGG 2 cut(s) 216, 306
PspN4I GGNNCC 6 cut(s) 151, 248, 249, 824, 906, 907
PspPI GGNCC 8 cut(s) 235, 247, 313, 353, 434, 640, 722, 905
PspPPI RGGWCCY 2 cut(s) 247, 905
PstI CTGCAG 2 cut(s) 780, 861
PstNI CAGNNNCTG 1 cut(s) 295
PsuI RGATCY 2 cut(s) 168, 505
PsyI GACNNNGTC 2 cut(s) 206, 251
PvuII CAGCTG 5 cut(s) 295, 493, 775, 793, 856
RsaI GTAC 2 cut(s) 443, 728
RsaNI GTAC 2 cut(s) 442, 727
Rsr2I CGGWCCG 2 cut(s) 434, 722
RsrII CGGWCCG 2 cut(s) 434, 722
SacI GAGCTC 2 cut(s) 183, 339
Sau3AI GATC 3 cut(s) 168, 505, 604
Sau96I GGNCC 8 cut(s) 235, 247, 313, 353, 434, 640, 722, 905
SchI GAGTC 1 cut(s) 264
ScrFI CCNGG 4 cut(s) 218, 245, 308, 876
SduI GDGCHC 4 cut(s) 74, 183, 339, 825
SfaNI GCATC 1 cut(s) 535
SfcI CTRYAG 2 cut(s) 776, 857
SfoI GGCGCC 1 cut(s) 151
SinI GGWCC 6 cut(s) 247, 313, 434, 640, 722, 905
Sse9I AATT 1 cut(s) 53
SspDI GGCGCC 1 cut(s) 149
SstI GAGCTC 2 cut(s) 183, 339
StyD4I CCNGG 4 cut(s) 216, 243, 306, 874
TaiI ACGT 1 cut(s) 135
TaqI TCGA 2 cut(s) 502, 540
TasI AATT 1 cut(s) 53
TauI GCSGC 7 cut(s) 385, 479, 560, 563, 572, 745, 817
TfiI GAWTC 1 cut(s) 736
TspGWI ACGGA 2 cut(s) 27, 714
Tth111I GACNNNGTC 2 cut(s) 206, 251
VneI GTGCAC 1 cut(s) 70
VpaK11BI GGWCC 6 cut(s) 247, 313, 434, 640, 722, 905
XapI RAATTY 1 cut(s) 53
XceI RCATGY 1 cut(s) 451
XmnI GAANNNNTTC 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.