MD15G1279500.v1.1

LOB domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008830
Physical Location & Seq
Reverse (-)
24853281 .. 24857116
3836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1279500.v1.1.491

Sequence Viewer

Length: 939 bp
ATGTCTTCGTCGTCGTCAAACTCTCCTTGCGCAGCGTGCAAATTTCTGCGGCGGAAGTGCACGCAGGAGTGCGTCTTCGCGCCTTACTTCCCACCTGACAATCCCCATAAGTTTTCCAAAGTCCACAAGGTCTTCGGTGCCAGCAATGTCGCCAAGATCCTCAACGAGCTCAGCGCTTCTCAGCGCGATGACGCCGTCAACTCTCTGGCCTATGAGGCGGAGGCCCGCCTTCGCGACCCCGTCTACGGCTGCGTTGGACTCATTTCCATCCTCCAGCAGCGGCTCAAGGAAGTCCAGGCCGACCTTTTCAATGCCAAGAAGGAGCTCGCCACTTACATGGGCCCACAAGGTATGCTCTCCATCCTCCAGCCGCCAACTTACATGGCTCAGCAGCTCCAAGGAAACGCTTCTTCCGCCGTTTCGCCGTACATGTCTCCAATGATTGGCATTCCAACCGGGGCTGCTTCCGGGCAGTTGATGATTCGAGAGCCACAGCAACAACAGATTTTTGAGGCGCAGCAGTTGGCGGTGGCCGTGGCAGCGAGGGAGCAGCAGGAGATGTTCAGGAGTTTTGATCAGAGTAAACAGGCAATGCAGCAGCAGGCGCAGGACCATGTGAGATTCAACAGTGGGTTTGACTCGTCCCCTGGCTCGGTCACTGTGGGCGGCTTCACTCAGATGGCCGGAGCGTTGGGCAACGCCGTTTCGCCGTCTTTGGCTCTGGGGTTTGATAGCGGTCCGTACCACCAGATTCAGCAGCAGCAGCCTCACCATAATCCCCACCTCCACCTCCACCTGCAGCTTCAGTCTCAGTTGTTGCTCCAAACCCAACAGCCGCAAGGGCAGCATCAGGCTCAGCAGCCCCAGCAACCCCAGTTGCAGGCAAAGTCCGATCTGGGCGAGGAAGGCGGTGGTAGGAGTGTGGGTCCCTCTTGTTGA

Protein Analysis

313

Amino Acids

33.96

Weight (kDa)

7.14

Isoelectric Point (pI)

75.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 9 - 106 6.1e-41 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 804
Acc16I TGCGCA 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 804
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 1 cut(s) 243
AccII CGCG 3 cut(s) 80, 186, 234
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 2 cut(s) 531, 681
AcsI RAATTY 1 cut(s) 41
AcuI CTGAAG 1 cut(s) 788
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 2 cut(s) 428, 743
AfeI AGCGCT 1 cut(s) 175
AfiI CCNNNNNNNGG 4 cut(s) 245, 443, 652, 880
AflIII ACRYGT 1 cut(s) 429
AgsI TTSAA 2 cut(s) 310, 625
AjnI CCWGG 2 cut(s) 294, 646
AluBI AGCT 4 cut(s) 169, 325, 394, 802
AluI AGCT 4 cut(s) 169, 325, 394, 802
Alw21I GWGCWC 3 cut(s) 62, 171, 327
Alw26I GTCTC 2 cut(s) 438, 813
Alw44I GTGCAC 1 cut(s) 58
AlwI GGATC 1 cut(s) 151
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 6 cut(s) 207, 222, 297, 340, 531, 681
ApaI GGGCCC 1 cut(s) 344
ApaLI GTGCAC 1 cut(s) 58
ApoI RAATTY 1 cut(s) 41
Asp700I GAANNNNTTC 1 cut(s) 406
AspLEI GCGC 6 cut(s) 32, 82, 176, 186, 517, 607
AspS9I GGNCC 6 cut(s) 223, 340, 341, 610, 737, 926
AsuC2I CCSGG 2 cut(s) 457, 469
AsuHPI GGTGA 1 cut(s) 761
AvaII GGWCC 3 cut(s) 610, 737, 926
BaeGI GKGCMC 2 cut(s) 62, 344
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 3 cut(s) 171, 327, 344
BbsI GAAGAC 2 cut(s) 67, 124
Bbv12I GWGCWC 3 cut(s) 62, 171, 327
BccI CCATC 3 cut(s) 275, 368, 673
BceAI ACGGC 7 cut(s) 179, 262, 401, 409, 518, 686, 694
BciT130I CCWGG 2 cut(s) 296, 648
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 2 cut(s) 457, 469
BcoDI GTCTC 2 cut(s) 438, 813
BfmI CTRYAG 1 cut(s) 797
BfoI RGCGCY 1 cut(s) 177
BfuAI ACCTGC 1 cut(s) 804
BglI GCCNNNNNGGC 2 cut(s) 215, 841
BlpI GCTNAGC 3 cut(s) 170, 387, 855
Bme1390I CCNGG 4 cut(s) 296, 457, 469, 648
Bme18I GGWCC 3 cut(s) 610, 737, 926
BmgT120I GGNCC 6 cut(s) 223, 340, 341, 610, 737, 926
BmiI GGNNCC 4 cut(s) 139, 342, 927, 928
BmrFI CCNGG 4 cut(s) 296, 457, 469, 648
BmrI ACTGGG 1 cut(s) 868
BmsI GCATC 1 cut(s) 856
BmuI ACTGGG 1 cut(s) 868
BpiI GAAGAC 2 cut(s) 67, 124
BpmI CTGGAG 2 cut(s) 257, 350
Bpu1102I GCTNAGC 3 cut(s) 170, 387, 855
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 2 cut(s) 457, 469
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 397, 456, 534, 646
BsaXI ACNNNNNCTCC 1 cut(s) 910
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 443, 652, 880
Bse1I ACTGG 1 cut(s) 874
Bse3DI GCAATG 2 cut(s) 151, 597
BseBI CCWGG 2 cut(s) 296, 648
BseDI CCNNGG 4 cut(s) 397, 456, 534, 646
BseGI GGATG 2 cut(s) 267, 360
BseLI CCNNNNNNNGG 4 cut(s) 245, 443, 652, 880
BseMI GCAATG 2 cut(s) 151, 597
BseMII CTCAG 6 cut(s) 184, 194, 401, 689, 824, 869
BseNI ACTGG 1 cut(s) 874
BseSI GKGCMC 2 cut(s) 62, 344
BseYI CCCAGC 1 cut(s) 864
Bsh1236I CGCG 3 cut(s) 80, 186, 234
BshFI GGCC 6 cut(s) 209, 224, 299, 342, 533, 683
BshNI GGYRCC 1 cut(s) 137
BsiHKAI GWGCWC 3 cut(s) 62, 171, 327
BsiSI CCGG 3 cut(s) 456, 468, 684
BslFI GGGAC 2 cut(s) 628, 912
BslI CCNNNNNNNGG 4 cut(s) 245, 443, 652, 880
BsmAI GTCTC 2 cut(s) 438, 813
BsmFI GGGAC 2 cut(s) 628, 912
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 6 cut(s) 209, 224, 299, 342, 533, 683
Bsp120I GGGCCC 1 cut(s) 340
Bsp1286I GDGCHC 4 cut(s) 62, 171, 327, 344
Bsp143I GATC 3 cut(s) 156, 574, 892
Bsp1720I GCTNAGC 3 cut(s) 170, 387, 855
Bsp68I TCGCGA 1 cut(s) 234
BspANI GGCC 6 cut(s) 209, 224, 299, 342, 533, 683
BspCNI CTCAG 6 cut(s) 183, 193, 400, 688, 823, 868
BspFNI CGCG 3 cut(s) 80, 186, 234
BspLI GGNNCC 4 cut(s) 139, 342, 927, 928
BspMAI CTGCAG 1 cut(s) 801
BspMI ACCTGC 1 cut(s) 804
BspPI GGATC 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 137
BsrDI GCAATG 2 cut(s) 151, 597
BsrI ACTGG 1 cut(s) 874
BssECI CCNNGG 4 cut(s) 397, 456, 534, 646
BssMI GATC 3 cut(s) 156, 574, 892
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 397
Bst2UI CCWGG 2 cut(s) 296, 648
Bst4CI ACNGT 2 cut(s) 629, 661
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 7 cut(s) 37, 62, 142, 226, 327, 603, 882
BstDEI CTNAG 6 cut(s) 170, 180, 387, 675, 810, 855
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 267, 360
BstFNI CGCG 3 cut(s) 80, 186, 234
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 6 cut(s) 32, 82, 176, 186, 517, 607
BstKTI GATC 3 cut(s) 159, 577, 895
BstMAI GTCTC 2 cut(s) 438, 813
BstMBI GATC 3 cut(s) 156, 574, 892
BstNI CCWGG 2 cut(s) 296, 648
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 4 cut(s) 294, 455, 467, 646
BstSFI CTRYAG 1 cut(s) 797
BstSLI GKGCMC 2 cut(s) 62, 344
BstUI CGCG 3 cut(s) 80, 186, 234
BstV2I GAAGAC 2 cut(s) 67, 124
BstX2I RGATCY 1 cut(s) 156
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 1 cut(s) 156
BsuRI GGCC 6 cut(s) 209, 224, 299, 342, 533, 683
BtgI CCRYGG 1 cut(s) 534
BtgZI GCGATG 1 cut(s) 201
BtsCI GGATG 2 cut(s) 267, 360
BtsIMutI CAGTG 2 cut(s) 634, 657
BtuMI TCGCGA 1 cut(s) 234
BveI ACCTGC 1 cut(s) 804
Cac8I GCNNGC 7 cut(s) 37, 62, 142, 226, 327, 603, 882
CfoI GCGC 6 cut(s) 32, 82, 176, 186, 517, 607
Cfr13I GGNCC 6 cut(s) 223, 340, 341, 610, 737, 926
CpoI CGGWCCG 1 cut(s) 737
CseI GACGC 2 cut(s) 61, 200
Csp6I GTAC 2 cut(s) 427, 742
CspI CGGWCCG 1 cut(s) 737
CviAII CATG 4 cut(s) 337, 382, 430, 614
CviQI GTAC 2 cut(s) 427, 742
DdeI CTNAG 6 cut(s) 170, 180, 387, 675, 810, 855
DpnI GATC 3 cut(s) 158, 576, 894
DpnII GATC 3 cut(s) 156, 574, 892
EaeI YGGCCR 2 cut(s) 531, 681
EciI GGCGGA 3 cut(s) 67, 233, 403
Ecl136II GAGCTC 2 cut(s) 169, 325
Eco130I CCWWGG 1 cut(s) 397
Eco24I GRGCYC 3 cut(s) 171, 327, 344
Eco47I GGWCC 3 cut(s) 610, 737, 926
Eco47III AGCGCT 1 cut(s) 175
Eco53kI GAGCTC 2 cut(s) 169, 325
Eco57I CTGAAG 1 cut(s) 788
EcoICRI GAGCTC 2 cut(s) 169, 325
EcoO109I RGGNCCY 1 cut(s) 926
EcoRII CCWGG 2 cut(s) 294, 646
EcoT14I CCWWGG 1 cut(s) 397
EcoT38I GRGCYC 3 cut(s) 171, 327, 344
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 4 cut(s) 340, 385, 433, 617
FaiI YATR 8 cut(s) 108, 213, 338, 353, 383, 431, 615, 774
FaqI GGGAC 2 cut(s) 628, 912
FatI CATG 4 cut(s) 336, 381, 429, 613
FauI CCCGC 1 cut(s) 233
FbaI TGATCA 1 cut(s) 574
FblI GTMKAC 1 cut(s) 243
FokI GGATG 2 cut(s) 254, 347
FriOI GRGCYC 3 cut(s) 171, 327, 344
FspI TGCGCA 1 cut(s) 31
GlaI GCGC 6 cut(s) 31, 81, 175, 185, 516, 606
GsaI CCCAGC 1 cut(s) 868
GsuI CTGGAG 2 cut(s) 257, 350
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 6 cut(s) 209, 224, 299, 342, 533, 683
HapII CCGG 3 cut(s) 456, 468, 684
HgaI GACGC 2 cut(s) 61, 200
HhaI GCGC 6 cut(s) 32, 82, 176, 186, 517, 607
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 4 cut(s) 340, 385, 433, 617
Hin6I GCGC 6 cut(s) 30, 80, 174, 184, 515, 605
HinP1I GCGC 6 cut(s) 30, 80, 174, 184, 515, 605
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 5 cut(s) 258, 481, 621, 638, 751
HpaII CCGG 3 cut(s) 456, 468, 684
HphI GGTGA 1 cut(s) 761
Hpy166II GTNNAC 5 cut(s) 60, 124, 199, 244, 584
Hpy188I TCNGA 3 cut(s) 579, 678, 892
Hpy188III TCNNGA 3 cut(s) 233, 485, 565
Hpy8I GTNNAC 5 cut(s) 60, 124, 199, 244, 584
Hpy99I CGWCG 2 cut(s) 13, 16
HpyAV CCTTC 3 cut(s) 239, 313, 899
HpyCH4III ACNGT 2 cut(s) 629, 661
HpyCH4V TGCA 5 cut(s) 39, 60, 595, 799, 880
HpyF3I CTNAG 6 cut(s) 170, 180, 387, 675, 810, 855
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 4 cut(s) 340, 385, 433, 617
HspAI GCGC 6 cut(s) 30, 80, 174, 184, 515, 605
KflI GGGWCCC 1 cut(s) 926
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 3 cut(s) 156, 574, 892
LmnI GCTCC 5 cut(s) 322, 399, 547, 686, 825
LweI GCATC 1 cut(s) 856
MaeIII GTNAC 1 cut(s) 655
MalI GATC 3 cut(s) 158, 576, 894
MboI GATC 3 cut(s) 156, 574, 892
MboII GAAGA 3 cut(s) 67, 124, 402
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 4 cut(s) 62, 171, 327, 344
MluCI AATT 1 cut(s) 41
MlyI GAGTC 2 cut(s) 252, 632
MmeI TCCRAC 2 cut(s) 235, 476
MroXI GAANNNNTTC 1 cut(s) 406
MslI CAYNNNNRTG 2 cut(s) 335, 677
MspA1I CMGCKG 1 cut(s) 280
MspI CCGG 3 cut(s) 456, 468, 684
MspR9I CCNGG 4 cut(s) 296, 457, 469, 648
Mva1269I GAATGC 1 cut(s) 447
MvaI CCWGG 2 cut(s) 296, 648
MvnI CGCG 3 cut(s) 80, 186, 234
NciI CCSGG 2 cut(s) 457, 469
NdeII GATC 3 cut(s) 156, 574, 892
NlaIII CATG 4 cut(s) 340, 385, 433, 617
NlaIV GGNNCC 4 cut(s) 139, 342, 927, 928
NmuCI GTSAC 1 cut(s) 655
NruI TCGCGA 1 cut(s) 234
NsbI TGCGCA 1 cut(s) 31
NspI RCATGY 1 cut(s) 433
PaqCI CACCTGC 1 cut(s) 804
PciI ACATGT 1 cut(s) 429
PctI GAATGC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 406
PfeI GAWTC 3 cut(s) 481, 621, 751
PflFI GACNNNGTC 2 cut(s) 194, 239
PflMI CCANNNNNTGG 1 cut(s) 443
PleI GAGTC 2 cut(s) 252, 632
PpsI GAGTC 2 cut(s) 252, 632
PpuMI RGGWCCY 1 cut(s) 926
PscI ACATGT 1 cut(s) 429
Psp124BI GAGCTC 2 cut(s) 171, 327
Psp5II RGGWCCY 1 cut(s) 926
Psp6I CCWGG 2 cut(s) 294, 646
PspFI CCCAGC 1 cut(s) 864
PspGI CCWGG 2 cut(s) 294, 646
PspN4I GGNNCC 4 cut(s) 139, 342, 927, 928
PspOMI GGGCCC 1 cut(s) 340
PspPI GGNCC 6 cut(s) 223, 340, 341, 610, 737, 926
PspPPI RGGWCCY 1 cut(s) 926
PstI CTGCAG 1 cut(s) 801
PsuI RGATCY 1 cut(s) 156
PsyI GACNNNGTC 2 cut(s) 194, 239
RruI TCGCGA 1 cut(s) 234
RsaI GTAC 2 cut(s) 428, 743
RsaNI GTAC 2 cut(s) 427, 742
RseI CAYNNNNRTG 2 cut(s) 335, 677
Rsr2I CGGWCCG 1 cut(s) 737
RsrII CGGWCCG 1 cut(s) 737
SacI GAGCTC 2 cut(s) 171, 327
Sau3AI GATC 3 cut(s) 156, 574, 892
Sau96I GGNCC 6 cut(s) 223, 340, 341, 610, 737, 926
SchI GAGTC 2 cut(s) 252, 632
ScrFI CCNGG 4 cut(s) 296, 457, 469, 648
SduI GDGCHC 4 cut(s) 62, 171, 327, 344
SfaNI GCATC 1 cut(s) 856
SfcI CTRYAG 1 cut(s) 797
SinI GGWCC 3 cut(s) 610, 737, 926
SmiMI CAYNNNNRTG 2 cut(s) 335, 677
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 1 cut(s) 41
SstI GAGCTC 2 cut(s) 171, 327
StyD4I CCNGG 4 cut(s) 294, 455, 467, 646
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 2 cut(s) 629, 661
TaqI TCGA 1 cut(s) 484
TaqII GACCGA 1 cut(s) 643
TasI AATT 1 cut(s) 41
TauI GCSGC 5 cut(s) 52, 283, 373, 669, 838
TfiI GAWTC 3 cut(s) 481, 621, 751
TscAI CASTG 2 cut(s) 634, 664
TseFI GTSAC 1 cut(s) 655
Tsp45I GTSAC 1 cut(s) 655
TspGWI ACGGA 1 cut(s) 729
TspRI CASTG 2 cut(s) 634, 664
Tth111I GACNNNGTC 2 cut(s) 194, 239
Van91I CCANNNNNTGG 1 cut(s) 443
VneI GTGCAC 1 cut(s) 58
VpaK11BI GGWCC 3 cut(s) 610, 737, 926
XapI RAATTY 1 cut(s) 41
XceI RCATGY 1 cut(s) 433
XmiI GTMKAC 1 cut(s) 243
XmnI GAANNNNTTC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.