MD02G1176200.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014979
Physical Location & Seq
Reverse (-)
15549167 .. 15549643
477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1176200.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGAGGGCGGCGGCGGAGAGGCTGCGAATAGGAAGAGAGCTGGGGTGGACAACGACAAGCCGTACAAGGGGATACGGATGAGAAAGTGGGGCAAGTGGGTGGCCGAGATTCGAGAACCCAACAAGCGCTCTAGAATCTGGCTTGGTTCCTACTCCACTCCTGTCGCCGCCGCTCGGGCGTACGACACGGCGGTTTTTTACTTGCGGGGTCCCTCCGCGCGGCTCAATTTTCCAGAGGCTTTGCCGGTCGAAGGTGGCTGCGGGTGCGGTGACATGTCGGCAGCTTTGATACGCAAGCGGGCCACCGAGGTCGGCGCCCGGGTAGACGCGGTCGAAACGGCTCTGCGACACCACCGCGGTTCGGCGGCAGGGAACGGAGACGGCGATGAGATTAAACCCTGCTTCGCCGGATGCGTGGATCGGGTCGACCTGAACAAGATGCCCGACCCGGAGAATTCGGAAGGTGAGTGGAATTAG

Protein Analysis

159

Amino Acids

17.13

Weight (kDa)

8.72

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 22 - 70 4.6e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23340 AT3G50260
fragaria_vesca FvH4_1g16370
malus_domestica MD02G1176200.v1.1 MD15G1286600.v1.1
prunus_persica Prupe.6G231500_v2.0.a1
pyrus_communis pycom02g14240 pycom15g24990
rosa_chinensis RchiOBHm_Chr2g0106241
rosa_laevigata RLG00000017482
rosa_multiflora Rmu_sc0008603.1_g000012
rosa_roxburghii Rroxscaffold_2G00137340
rosa_rugosa Rorug02G0133700
rosa_samantha Rh2AG184800 Rh2CG189900
rosa_wichuraiana Rw2G014430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 314
AccBSI CCGCTC 1 cut(s) 173
AccI GTMKAC 2 cut(s) 324, 426
AccII CGCG 4 cut(s) 218, 220, 329, 357
AclWI GGATC 1 cut(s) 426
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 454
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 2 cut(s) 65, 182
AfeI AGCGCT 1 cut(s) 128
AfiI CCNNNNNNNGG 5 cut(s) 68, 174, 219, 251, 361
AflIII ACRYGT 1 cut(s) 273
AluBI AGCT 2 cut(s) 41, 284
AluI AGCT 2 cut(s) 41, 284
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 1 cut(s) 426
Ama87I CYCGRG 2 cut(s) 174, 318
Aor51HI AGCGCT 1 cut(s) 128
AoxI GGCC 2 cut(s) 102, 300
ApeKI GCWGC 3 cut(s) 23, 258, 281
ApoI RAATTY 1 cut(s) 454
AspLEI GCGC 3 cut(s) 129, 220, 317
AspS9I GGNCC 2 cut(s) 209, 300
AsuC2I CCSGG 3 cut(s) 319, 320, 449
AsuHPI GGTGA 2 cut(s) 281, 476
AvaI CYCGRG 2 cut(s) 174, 318
AvaII GGWCC 1 cut(s) 209
BanI GGYRCC 1 cut(s) 314
BbvI GCAGC 3 cut(s) 10, 245, 293
BceAI ACGGC 4 cut(s) 46, 204, 354, 397
BciVI GTATCC 1 cut(s) 66
BcnI CCSGG 3 cut(s) 319, 320, 449
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 1 cut(s) 132
BfoI RGCGCY 2 cut(s) 130, 318
BfuI GTATCC 1 cut(s) 66
BglI GCCNNNNNGGC 1 cut(s) 176
BisI GCNGC 9 cut(s) 10, 13, 24, 168, 171, 221, 259, 282, 366
BlsI GCNGC 9 cut(s) 11, 14, 25, 169, 172, 222, 260, 283, 367
Bme1390I CCNGG 3 cut(s) 319, 320, 449
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 2 cut(s) 174, 318
BmgT120I GGNCC 2 cut(s) 209, 300
BmiI GGNNCC 4 cut(s) 148, 210, 211, 316
BmrFI CCNGG 3 cut(s) 319, 320, 449
BmsI GCATC 2 cut(s) 401, 429
BpuMI CCSGG 3 cut(s) 319, 320, 449
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 3 cut(s) 306, 318, 355
Bsc4I CCNNNNNNNGG 5 cut(s) 68, 174, 219, 251, 361
Bse118I RCCGGY 1 cut(s) 244
BseDI CCNNGG 3 cut(s) 306, 318, 355
BseGI GGATG 2 cut(s) 84, 416
BseLI CCNNNNNNNGG 5 cut(s) 68, 174, 219, 251, 361
BseXI GCAGC 3 cut(s) 10, 245, 293
BseYI CCCAGC 1 cut(s) 41
Bsh1236I CGCG 4 cut(s) 218, 220, 329, 357
Bsh1285I CGRYCG 2 cut(s) 249, 333
BshFI GGCC 2 cut(s) 104, 302
BshNI GGYRCC 1 cut(s) 314
BsiEI CGRYCG 2 cut(s) 249, 333
BsiHKCI CYCGRG 2 cut(s) 174, 318
BsiSI CCGG 4 cut(s) 245, 319, 408, 449
BsiWI CGTACG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 5 cut(s) 68, 174, 219, 251, 361
BsmAI GTCTC 1 cut(s) 372
BsmBI CGTCTC 1 cut(s) 372
BsmFI GGGAC 1 cut(s) 195
BsnI GGCC 2 cut(s) 104, 302
BsoBI CYCGRG 2 cut(s) 174, 318
Bsp143I GATC 1 cut(s) 418
BspANI GGCC 2 cut(s) 104, 302
BspFNI CGCG 4 cut(s) 218, 220, 329, 357
BspLI GGNNCC 4 cut(s) 148, 210, 211, 316
BspPI GGATC 1 cut(s) 426
BspT107I GGYRCC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 244
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 3 cut(s) 306, 318, 355
BssMI GATC 1 cut(s) 418
BssNI GRCGYC 1 cut(s) 315
Bst6I CTCTTC 1 cut(s) 29
BstACI GRCGYC 1 cut(s) 315
BstC8I GCNNGC 2 cut(s) 296, 300
BstDSI CCRYGG 1 cut(s) 355
BstF5I GGATG 2 cut(s) 84, 416
BstFNI CGCG 4 cut(s) 218, 220, 329, 357
BstH2I RGCGCY 2 cut(s) 130, 318
BstHHI GCGC 3 cut(s) 129, 220, 317
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 1 cut(s) 418
BstMCI CGRYCG 2 cut(s) 249, 333
BstMWI GCNNNNNNNGC 2 cut(s) 176, 264
BstNSI RCATGY 1 cut(s) 277
BstSCI CCNGG 3 cut(s) 317, 318, 447
BstUI CGCG 4 cut(s) 218, 220, 329, 357
BstV1I GCAGC 3 cut(s) 10, 245, 293
BsuI GTATCC 1 cut(s) 66
BsuRI GGCC 2 cut(s) 104, 302
BtgI CCRYGG 1 cut(s) 355
BtgZI GCGATG 1 cut(s) 399
BtsCI GGATG 2 cut(s) 84, 416
Cac8I GCNNGC 2 cut(s) 296, 300
CfoI GCGC 3 cut(s) 129, 220, 317
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 2 cut(s) 209, 300
Cfr42I CCGCGG 1 cut(s) 358
Cfr9I CCCGGG 1 cut(s) 318
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 64, 181
CviAII CATG 1 cut(s) 274
CviQI GTAC 2 cut(s) 64, 181
DinI GGCGCC 1 cut(s) 316
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
EciI GGCGGA 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 209
Eco47III AGCGCT 1 cut(s) 128
Eco88I CYCGRG 2 cut(s) 174, 318
EcoO109I RGGNCCY 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 454
EgeI GGCGCC 1 cut(s) 316
EheI GGCGCC 1 cut(s) 316
Esp3I CGTCTC 1 cut(s) 372
FaeI CATG 1 cut(s) 277
FaiI YATR 1 cut(s) 275
FaqI GGGAC 1 cut(s) 195
FatI CATG 1 cut(s) 273
FauI CCCGC 3 cut(s) 198, 254, 291
FblI GTMKAC 2 cut(s) 324, 426
Fnu4HI GCNGC 9 cut(s) 10, 13, 24, 168, 171, 221, 259, 282, 366
FokI GGATG 2 cut(s) 91, 423
Fsp4HI GCNGC 9 cut(s) 10, 13, 24, 168, 171, 221, 259, 282, 366
FspBI CTAG 1 cut(s) 132
GlaI GCGC 3 cut(s) 128, 219, 316
GluI GCNGC 9 cut(s) 10, 13, 24, 168, 171, 221, 259, 282, 366
GsaI CCCAGC 1 cut(s) 45
HaeII RGCGCY 2 cut(s) 130, 318
HaeIII GGCC 2 cut(s) 104, 302
HapII CCGG 4 cut(s) 245, 319, 408, 449
HgaI GACGC 1 cut(s) 335
HhaI GCGC 3 cut(s) 129, 220, 317
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 1 cut(s) 277
Hin6I GCGC 3 cut(s) 127, 218, 315
HinP1I GCGC 3 cut(s) 127, 218, 315
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 2 cut(s) 109, 135
HpaII CCGG 4 cut(s) 245, 319, 408, 449
HphI GGTGA 2 cut(s) 281, 476
Hpy166II GTNNAC 3 cut(s) 49, 325, 427
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 3 cut(s) 113, 132, 233
Hpy8I GTNNAC 3 cut(s) 49, 325, 427
HpyAV CCTTC 2 cut(s) 245, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 264
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 1 cut(s) 277
HspAI GCGC 3 cut(s) 127, 218, 315
KasI GGCGCC 1 cut(s) 314
KflI GGGWCCC 1 cut(s) 209
KspI CCGCGG 1 cut(s) 358
Kzo9I GATC 1 cut(s) 418
Lsp1109I GCAGC 3 cut(s) 10, 245, 293
LweI GCATC 2 cut(s) 401, 429
MaeI CTAG 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 269
MalI GATC 1 cut(s) 420
MbiI CCGCTC 1 cut(s) 173
MboI GATC 1 cut(s) 418
MboII GAAGA 1 cut(s) 46
MluCI AATT 3 cut(s) 226, 454, 472
Mly113I GGCGCC 1 cut(s) 315
MnlI CCTC 4 cut(s) 13, 223, 229, 301
MseI TTAA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 357
MspI CCGG 4 cut(s) 245, 319, 408, 449
MspR9I CCNGG 3 cut(s) 319, 320, 449
MvnI CGCG 4 cut(s) 218, 220, 329, 357
MwoI GCNNNNNNNGC 2 cut(s) 176, 264
NarI GGCGCC 1 cut(s) 315
NciI CCSGG 3 cut(s) 319, 320, 449
NdeII GATC 1 cut(s) 418
NlaIII CATG 1 cut(s) 277
NlaIV GGNNCC 4 cut(s) 148, 210, 211, 316
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 269
NspI RCATGY 1 cut(s) 277
PciI ACATGT 1 cut(s) 273
PcsI WCGNNNNNNNCGW 2 cut(s) 381, 411
PfeI GAWTC 2 cut(s) 109, 135
Pfl23II CGTACG 1 cut(s) 180
PflFI GACNNNGTC 1 cut(s) 329
PkrI GCNGC 9 cut(s) 11, 14, 25, 169, 172, 222, 260, 283, 367
PluTI GGCGCC 1 cut(s) 318
PpuMI RGGWCCY 1 cut(s) 209
PscI ACATGT 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 209
PspFI CCCAGC 1 cut(s) 41
PspLI CGTACG 1 cut(s) 180
PspN4I GGNNCC 4 cut(s) 148, 210, 211, 316
PspPI GGNCC 2 cut(s) 209, 300
PspPPI RGGWCCY 1 cut(s) 209
PsyI GACNNNGTC 1 cut(s) 329
RsaI GTAC 2 cut(s) 65, 182
RsaNI GTAC 2 cut(s) 64, 181
SacII CCGCGG 1 cut(s) 358
SalI GTCGAC 1 cut(s) 425
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 9 cut(s) 10, 13, 24, 168, 171, 221, 259, 282, 366
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 2 cut(s) 209, 300
ScrFI CCNGG 3 cut(s) 319, 320, 449
SetI ASST 6 cut(s) 43, 256, 286, 312, 432, 466
SfaNI GCATC 2 cut(s) 401, 429
SfoI GGCGCC 1 cut(s) 316
Sfr303I CCGCGG 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 358
SinI GGWCC 1 cut(s) 209
SmaI CCCGGG 1 cut(s) 320
Sse9I AATT 3 cut(s) 226, 454, 472
SspDI GGCGCC 1 cut(s) 314
SspMI CTAG 1 cut(s) 132
StyD4I CCNGG 3 cut(s) 317, 318, 447
TaqI TCGA 4 cut(s) 112, 249, 333, 426
TasI AATT 3 cut(s) 226, 454, 472
TauI GCSGC 6 cut(s) 12, 15, 170, 173, 223, 368
TfiI GAWTC 2 cut(s) 109, 135
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 3 cut(s) 23, 258, 281
Tsp45I GTSAC 1 cut(s) 269
TspGWI ACGGA 2 cut(s) 91, 390
TspMI CCCGGG 1 cut(s) 318
Tth111I GACNNNGTC 1 cut(s) 329
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 1 cut(s) 454
XbaI TCTAGA 1 cut(s) 131
XceI RCATGY 1 cut(s) 277
XmaI CCCGGG 1 cut(s) 318
XmiI GTMKAC 2 cut(s) 324, 426
XspI CTAG 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.