MD15G1286600.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014979
Physical Location & Seq
Reverse (-)
26242775 .. 26243658
884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1286600.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGAGTGCGGCGGAGACACGGCGAACAGGAAGAGAGCGGTGGAGAACGACAAGCCATACAAGGGGATAAGGATGAGGAAGTGGGGAAAGTGGGTGGCCGAGATTCGAGAACCCAACAAGCGCTCGCGAATTTGGCTCGGCTCCTACTCCACTCCCGTCGCCGCCGCCCGGGCGTACGATACGGCTGTTTTTTACTTGAGGGGTCCATCGGCGCGGCTCAATTTTCCAGAGGCTTTGGCGGTCGAAGGCGGCGGCTGCGGGTGCGGCGACATGTCGGCGGCCTCAATACGCAAACGGGCCACCGAGGTCGGTGCCCGGGTCGACGCGGTCGAGACCGCTATGCGAAACCACCGCGGTTCGGCCGGGGGGAACGGAGACGGCGCTGAGATTAAGCCTTGCGTCGCCGGATTCGTGGATCGGGTCGACTTGAACAAGATGCCCGACCCGGAGAACTCGGAAGGGGAGGTGTGTGACTGCTGA

Protein Analysis

160

Amino Acids

17.15

Weight (kDa)

8.55

Isoelectric Point (pI)

37.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 20 - 68 4.6e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23340 AT3G50260
fragaria_vesca FvH4_1g16370
malus_domestica MD02G1176200.v1.1 MD15G1286600.v1.1
prunus_persica Prupe.6G231500_v2.0.a1
pyrus_communis pycom02g14240 pycom15g24990
rosa_chinensis RchiOBHm_Chr2g0106241
rosa_laevigata RLG00000017482
rosa_multiflora Rmu_sc0008603.1_g000012
rosa_roxburghii Rroxscaffold_2G00137340
rosa_rugosa Rorug02G0133700
rosa_samantha Rh2AG184800 Rh2CG189900
rosa_wichuraiana Rw2G014430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 311
AccBSI CCGCTC 1 cut(s) 38
AccI GTMKAC 2 cut(s) 321, 423
AccII CGCG 4 cut(s) 127, 214, 326, 354
AclWI GGATC 1 cut(s) 423
AcoI YGGCCR 2 cut(s) 96, 360
AcsI RAATTY 1 cut(s) 129
AfaI GTAC 1 cut(s) 176
AfeI AGCGCT 1 cut(s) 122
AfiI CCNNNNNNNGG 3 cut(s) 62, 168, 358
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 1 cut(s) 430
Alw26I GTCTC 3 cut(s) 9, 326, 369
AlwI GGATC 1 cut(s) 423
Ama87I CYCGRG 2 cut(s) 168, 315
Aor51HI AGCGCT 1 cut(s) 122
AoxI GGCC 4 cut(s) 96, 279, 297, 360
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 1 cut(s) 129
AspLEI GCGC 3 cut(s) 123, 214, 383
AspS9I GGNCC 2 cut(s) 203, 297
AsuC2I CCSGG 6 cut(s) 169, 170, 316, 317, 364, 446
AvaI CYCGRG 2 cut(s) 168, 315
AvaII GGWCC 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 316
BaeI ACNNNNGTAYC 2 cut(s) 171, 204
BanI GGYRCC 1 cut(s) 311
BbvI GCAGC 1 cut(s) 242
BccI CCATC 1 cut(s) 214
BceAI ACGGC 3 cut(s) 36, 198, 394
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BcnI CCSGG 6 cut(s) 169, 170, 316, 317, 364, 446
BcoDI GTCTC 3 cut(s) 9, 326, 369
BfoI RGCGCY 2 cut(s) 124, 384
BglI GCCNNNNNGGC 1 cut(s) 170
BisI GCNGC 9 cut(s) 10, 162, 165, 215, 250, 253, 256, 265, 279
BlsI GCNGC 9 cut(s) 11, 163, 166, 216, 251, 254, 257, 266, 280
Bme1390I CCNGG 6 cut(s) 169, 170, 316, 317, 364, 446
Bme18I GGWCC 1 cut(s) 203
BmeT110I CYCGRG 2 cut(s) 168, 315
BmgT120I GGNCC 2 cut(s) 203, 297
BmiI GGNNCC 3 cut(s) 142, 204, 313
BmrFI CCNGG 6 cut(s) 169, 170, 316, 317, 364, 446
BmsI GCATC 1 cut(s) 426
BpuEI CTTGAG 1 cut(s) 217
BpuMI CCSGG 6 cut(s) 169, 170, 316, 317, 364, 446
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 5 cut(s) 168, 303, 315, 352, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 168, 358
BseDI CCNNGG 5 cut(s) 168, 303, 315, 352, 363
BseGI GGATG 1 cut(s) 78
BseLI CCNNNNNNNGG 3 cut(s) 62, 168, 358
BseMII CTCAG 1 cut(s) 375
BseSI GKGCMC 1 cut(s) 316
BseX3I CGGCCG 1 cut(s) 360
BseXI GCAGC 1 cut(s) 242
Bsh1236I CGCG 4 cut(s) 127, 214, 326, 354
Bsh1285I CGRYCG 3 cut(s) 243, 330, 363
BshFI GGCC 4 cut(s) 98, 281, 299, 362
BshNI GGYRCC 1 cut(s) 311
BsiEI CGRYCG 3 cut(s) 243, 330, 363
BsiHKCI CYCGRG 2 cut(s) 168, 315
BsiSI CCGG 5 cut(s) 169, 316, 363, 405, 446
BsiWI CGTACG 1 cut(s) 174
BslI CCNNNNNNNGG 3 cut(s) 62, 168, 358
BsmAI GTCTC 3 cut(s) 9, 326, 369
BsmBI CGTCTC 1 cut(s) 369
BsnI GGCC 4 cut(s) 98, 281, 299, 362
Bso31I GGTCTC 1 cut(s) 326
BsoBI CYCGRG 2 cut(s) 168, 315
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 1 cut(s) 415
Bsp68I TCGCGA 1 cut(s) 127
BspANI GGCC 4 cut(s) 98, 281, 299, 362
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 4 cut(s) 127, 214, 326, 354
BspLI GGNNCC 3 cut(s) 142, 204, 313
BspPI GGATC 1 cut(s) 423
BspT107I GGYRCC 1 cut(s) 311
BspTNI GGTCTC 1 cut(s) 326
BsrBI CCGCTC 1 cut(s) 38
BssECI CCNNGG 5 cut(s) 168, 303, 315, 352, 363
BssMI GATC 1 cut(s) 415
Bst6I CTCTTC 1 cut(s) 26
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 1 cut(s) 384
BstDSI CCRYGG 1 cut(s) 352
BstF5I GGATG 1 cut(s) 78
BstFNI CGCG 4 cut(s) 127, 214, 326, 354
BstH2I RGCGCY 2 cut(s) 124, 384
BstHHI GCGC 3 cut(s) 123, 214, 383
BstKTI GATC 1 cut(s) 418
BstMAI GTCTC 3 cut(s) 9, 326, 369
BstMBI GATC 1 cut(s) 415
BstMCI CGRYCG 3 cut(s) 243, 330, 363
BstMWI GCNNNNNNNGC 5 cut(s) 133, 170, 255, 261, 264
BstNSI RCATGY 1 cut(s) 274
BstSCI CCNGG 6 cut(s) 167, 168, 314, 315, 362, 444
BstSLI GKGCMC 1 cut(s) 316
BstUI CGCG 4 cut(s) 127, 214, 326, 354
BstV1I GCAGC 1 cut(s) 242
BstZI CGGCCG 1 cut(s) 360
BsuRI GGCC 4 cut(s) 98, 281, 299, 362
BtgI CCRYGG 1 cut(s) 352
BtsCI GGATG 1 cut(s) 78
BtuMI TCGCGA 1 cut(s) 127
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 3 cut(s) 123, 214, 383
Cfr13I GGNCC 2 cut(s) 203, 297
Cfr42I CCGCGG 1 cut(s) 355
Cfr9I CCCGGG 2 cut(s) 168, 315
CseI GACGC 2 cut(s) 332, 388
Csp6I GTAC 1 cut(s) 175
CviAII CATG 1 cut(s) 271
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 1 cut(s) 384
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
EaeI YGGCCR 2 cut(s) 96, 360
EagI CGGCCG 1 cut(s) 360
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 27
EclXI CGGCCG 1 cut(s) 360
Eco31I GGTCTC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 203
Eco47III AGCGCT 1 cut(s) 122
Eco52I CGGCCG 1 cut(s) 360
Eco88I CYCGRG 2 cut(s) 168, 315
Esp3I CGTCTC 1 cut(s) 369
FaeI CATG 1 cut(s) 274
FaiI YATR 3 cut(s) 58, 272, 341
FatI CATG 1 cut(s) 270
FauI CCCGC 1 cut(s) 251
FblI GTMKAC 2 cut(s) 321, 423
Fnu4HI GCNGC 9 cut(s) 10, 162, 165, 215, 250, 253, 256, 265, 279
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 9 cut(s) 10, 162, 165, 215, 250, 253, 256, 265, 279
GlaI GCGC 3 cut(s) 122, 213, 382
GluI GCNGC 9 cut(s) 10, 162, 165, 215, 250, 253, 256, 265, 279
HaeII RGCGCY 2 cut(s) 124, 384
HaeIII GGCC 4 cut(s) 98, 281, 299, 362
HapII CCGG 5 cut(s) 169, 316, 363, 405, 446
HgaI GACGC 2 cut(s) 332, 388
HhaI GCGC 3 cut(s) 123, 214, 383
Hin1II CATG 1 cut(s) 274
Hin6I GCGC 3 cut(s) 121, 212, 381
HinP1I GCGC 3 cut(s) 121, 212, 381
HincII GTYRAC 2 cut(s) 322, 424
HindII GTYRAC 2 cut(s) 322, 424
HinfI GANTC 2 cut(s) 103, 408
HpaII CCGG 5 cut(s) 169, 316, 363, 405, 446
Hpy166II GTNNAC 2 cut(s) 322, 424
Hpy188I TCNGA 1 cut(s) 457
Hpy188III TCNNGA 4 cut(s) 107, 126, 227, 331
Hpy8I GTNNAC 2 cut(s) 322, 424
Hpy99I CGWCG 3 cut(s) 161, 326, 404
HpyAV CCTTC 2 cut(s) 239, 452
HpyF10VI GCNNNNNNNGC 5 cut(s) 133, 170, 255, 261, 264
HpyF3I CTNAG 1 cut(s) 384
Hsp92II CATG 1 cut(s) 274
HspAI GCGC 3 cut(s) 121, 212, 381
KspI CCGCGG 1 cut(s) 355
Kzo9I GATC 1 cut(s) 415
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 7 cut(s) 13, 182, 240, 329, 376, 418, 459
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 1 cut(s) 426
MaeIII GTNAC 1 cut(s) 470
MalI GATC 1 cut(s) 417
MbiI CCGCTC 1 cut(s) 38
MboI GATC 1 cut(s) 415
MboII GAAGA 1 cut(s) 43
MhlI GDGCHC 1 cut(s) 316
MluCI AATT 2 cut(s) 129, 220
MnlI CCTC 6 cut(s) 69, 192, 223, 292, 298, 457
MseI TTAA 1 cut(s) 390
MspA1I CMGCKG 1 cut(s) 354
MspI CCGG 5 cut(s) 169, 316, 363, 405, 446
MspR9I CCNGG 6 cut(s) 169, 170, 316, 317, 364, 446
MvnI CGCG 4 cut(s) 127, 214, 326, 354
MwoI GCNNNNNNNGC 5 cut(s) 133, 170, 255, 261, 264
NciI CCSGG 6 cut(s) 169, 170, 316, 317, 364, 446
NdeII GATC 1 cut(s) 415
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 3 cut(s) 142, 204, 313
NmeAIII GCCGAG 2 cut(s) 117, 124
NmuCI GTSAC 1 cut(s) 470
NruI TCGCGA 1 cut(s) 127
NspI RCATGY 1 cut(s) 274
PciI ACATGT 1 cut(s) 270
PcsI WCGNNNNNNNCGW 2 cut(s) 327, 408
PfeI GAWTC 2 cut(s) 103, 408
Pfl23II CGTACG 1 cut(s) 174
PflFI GACNNNGTC 1 cut(s) 326
PkrI GCNGC 9 cut(s) 11, 163, 166, 216, 251, 254, 257, 266, 280
PscI ACATGT 1 cut(s) 270
PspLI CGTACG 1 cut(s) 174
PspN4I GGNNCC 3 cut(s) 142, 204, 313
PspPI GGNCC 2 cut(s) 203, 297
PsyI GACNNNGTC 1 cut(s) 326
RruI TCGCGA 1 cut(s) 127
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
SacII CCGCGG 1 cut(s) 355
SalI GTCGAC 2 cut(s) 320, 422
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 9 cut(s) 10, 162, 165, 215, 250, 253, 256, 265, 279
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 2 cut(s) 203, 297
ScrFI CCNGG 6 cut(s) 169, 170, 316, 317, 364, 446
SduI GDGCHC 1 cut(s) 316
SetI ASST 2 cut(s) 309, 468
SfaNI GCATC 1 cut(s) 426
Sfr303I CCGCGG 1 cut(s) 355
SgrBI CCGCGG 1 cut(s) 355
SinI GGWCC 1 cut(s) 203
SmaI CCCGGG 2 cut(s) 170, 317
SmlI CTYRAG 1 cut(s) 196
SmoI CTYRAG 1 cut(s) 196
SrfI GCCCGGGC 1 cut(s) 170
Sse9I AATT 2 cut(s) 129, 220
StyD4I CCNGG 6 cut(s) 167, 168, 314, 315, 362, 444
TaqI TCGA 5 cut(s) 106, 243, 321, 330, 423
TasI AATT 2 cut(s) 129, 220
TauI GCSGC 8 cut(s) 12, 164, 167, 217, 252, 255, 267, 281
TfiI GAWTC 2 cut(s) 103, 408
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 1 cut(s) 255
Tsp45I GTSAC 1 cut(s) 470
TspGWI ACGGA 1 cut(s) 387
TspMI CCCGGG 2 cut(s) 168, 315
Tth111I GACNNNGTC 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 203
XapI RAATTY 1 cut(s) 129
XceI RCATGY 1 cut(s) 274
XmaI CCCGGG 2 cut(s) 168, 315
XmiI GTMKAC 2 cut(s) 321, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.