Rw2G014430

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014979
Physical Location & Seq
Reverse (-)
16255920 .. 16256707
788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014430.1

Sequence Viewer

Length: 519 bp
ATGGAGGGTCAAGAAGCTGCGGCTGCGGCCAATAGGAAGAGAGGCGAGAACGACAAGCCGTACAAGGGGATAAGGATGAGGAAGTGGGGCAAGTGGGTGGCCGAGATTCGCGAGCCCAACAAGCGCTCCCGGATTTGGCTCGGGTCCTACTCCACTCCCGTCGCCGCCGCCCGCGCCTACGACACCGCCGTCTTCTACTTGAGGGGCCCGTCCGCCAGGCTCAATTTTCCCGAGGCGCTGCCGGTCGTCGGCGGAGGGTGCGGATGCGACGGCGACATGTCGGCGGCGTCGATAAGGAAGAAGGCCACCGAGGTCGGCGCCAGAGTCGACGCGGTCGAGACGGCCATGCGGCAACACAGGGCAGCGGCGGCGGGGGGTCCGATGGAGGAGATGAAGCCGTGTAGGGCGGCGGCGGGGGCGGAGGAGGAGAGCGGGTTCGTGGATCGGGTCGACTTGAACAAGATGCCCGACCCGGAGGAAACGGACGGCGGAGTCGTCTGCGATTGGGGGAGGAATTAA

Protein Analysis

172

Amino Acids

18.59

Weight (kDa)

8.35

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 21 - 69 5.4e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23340 AT3G50260
fragaria_vesca FvH4_1g16370
malus_domestica MD02G1176200.v1.1 MD15G1286600.v1.1
prunus_persica Prupe.6G231500_v2.0.a1
pyrus_communis pycom02g14240 pycom15g24990
rosa_chinensis RchiOBHm_Chr2g0106241
rosa_laevigata RLG00000017482
rosa_multiflora Rmu_sc0008603.1_g000012
rosa_roxburghii Rroxscaffold_2G00137340
rosa_rugosa Rorug02G0133700
rosa_samantha Rh2AG184800 Rh2CG189900
rosa_wichuraiana Rw2G014430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 188, 491
AccB1I GGYRCC 1 cut(s) 317
AccBSI CCGCTC 1 cut(s) 432
AccI GTMKAC 2 cut(s) 327, 450
AccII CGCG 3 cut(s) 111, 174, 332
AclWI GGATC 1 cut(s) 450
AcoI YGGCCR 3 cut(s) 27, 99, 342
AcyI GRCGYC 2 cut(s) 287, 318
AfaI GTAC 1 cut(s) 62
AfeI AGCGCT 1 cut(s) 125
AfiI CCNNNNNNNGG 3 cut(s) 65, 135, 248
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 1 cut(s) 457
AjnI CCWGG 1 cut(s) 215
AloI GAACNNNNNNTCC 2 cut(s) 419, 451
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 1 cut(s) 450
Ama87I CYCGRG 2 cut(s) 140, 230
Aor51HI AGCGCT 1 cut(s) 125
AoxI GGCC 5 cut(s) 27, 99, 205, 303, 342
ApaI GGGCCC 1 cut(s) 209
ApeKI GCWGC 4 cut(s) 17, 23, 238, 362
AspLEI GCGC 4 cut(s) 126, 176, 238, 320
AspS9I GGNCC 4 cut(s) 144, 205, 206, 377
AsuC2I CCSGG 2 cut(s) 130, 473
AvaI CYCGRG 2 cut(s) 140, 230
AvaII GGWCC 2 cut(s) 144, 377
BaeGI GKGCMC 1 cut(s) 209
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 2 cut(s) 117, 209
BbsI GAAGAC 1 cut(s) 184
BbvI GCAGC 4 cut(s) 4, 10, 225, 374
BccI CCATC 1 cut(s) 376
BceAI ACGGC 6 cut(s) 43, 173, 286, 357, 382, 502
BciT130I CCWGG 1 cut(s) 217
BcnI CCSGG 2 cut(s) 130, 473
BcoDI GTCTC 1 cut(s) 332
BfoI RGCGCY 3 cut(s) 127, 239, 321
Bme1390I CCNGG 3 cut(s) 130, 217, 473
Bme18I GGWCC 2 cut(s) 144, 377
BmeT110I CYCGRG 2 cut(s) 140, 230
BmgT120I GGNCC 4 cut(s) 144, 205, 206, 377
BmiI GGNNCC 5 cut(s) 145, 206, 207, 319, 378
BmrFI CCNGG 3 cut(s) 130, 217, 473
BmsI GCATC 2 cut(s) 254, 453
BpiI GAAGAC 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 220
BpuMI CCSGG 2 cut(s) 130, 473
BsaHI GRCGYC 2 cut(s) 287, 318
BsaJI CCNNGG 2 cut(s) 231, 309
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 419, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 135, 248
Bse118I RCCGGY 1 cut(s) 241
BseBI CCWGG 1 cut(s) 217
BseDI CCNNGG 2 cut(s) 231, 309
BseGI GGATG 2 cut(s) 81, 269
BseLI CCNNNNNNNGG 3 cut(s) 65, 135, 248
BseRI GAGGAG 3 cut(s) 401, 437, 440
BseSI GKGCMC 1 cut(s) 209
BseXI GCAGC 4 cut(s) 4, 10, 225, 374
Bsh1236I CGCG 3 cut(s) 111, 174, 332
Bsh1285I CGRYCG 2 cut(s) 246, 336
BshFI GGCC 5 cut(s) 29, 101, 207, 305, 344
BshNI GGYRCC 1 cut(s) 317
BsiEI CGRYCG 2 cut(s) 246, 336
BsiHKCI CYCGRG 2 cut(s) 140, 230
BsiSI CCGG 3 cut(s) 130, 242, 473
BslI CCNNNNNNNGG 3 cut(s) 65, 135, 248
BsmAI GTCTC 1 cut(s) 332
BsmBI CGTCTC 1 cut(s) 332
BsnI GGCC 5 cut(s) 29, 101, 207, 305, 344
BsoBI CYCGRG 2 cut(s) 140, 230
Bsp120I GGGCCC 1 cut(s) 205
Bsp1286I GDGCHC 2 cut(s) 117, 209
Bsp143I GATC 1 cut(s) 442
Bsp68I TCGCGA 1 cut(s) 111
BspANI GGCC 5 cut(s) 29, 101, 207, 305, 344
BspFNI CGCG 3 cut(s) 111, 174, 332
BspLI GGNNCC 5 cut(s) 145, 206, 207, 319, 378
BspPI GGATC 1 cut(s) 450
BspT107I GGYRCC 1 cut(s) 317
BsrBI CCGCTC 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 241
BssAI RCCGGY 1 cut(s) 241
BssECI CCNNGG 2 cut(s) 231, 309
BssMI GATC 1 cut(s) 442
BssNI GRCGYC 2 cut(s) 287, 318
Bst2UI CCWGG 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 2 cut(s) 287, 318
BstC8I GCNNGC 2 cut(s) 113, 172
BstF5I GGATG 2 cut(s) 81, 269
BstFNI CGCG 3 cut(s) 111, 174, 332
BstH2I RGCGCY 3 cut(s) 127, 239, 321
BstHHI GCGC 4 cut(s) 126, 176, 238, 320
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 1 cut(s) 442
BstMCI CGRYCG 2 cut(s) 246, 336
BstMWI GCNNNNNNNGC 7 cut(s) 23, 26, 121, 173, 258, 368, 416
BstNI CCWGG 1 cut(s) 217
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 3 cut(s) 128, 215, 471
BstSLI GKGCMC 1 cut(s) 209
BstUI CGCG 3 cut(s) 111, 174, 332
BstV1I GCAGC 4 cut(s) 4, 10, 225, 374
BstV2I GAAGAC 1 cut(s) 184
BsuRI GGCC 5 cut(s) 29, 101, 207, 305, 344
BtsCI GGATG 2 cut(s) 81, 269
BtuMI TCGCGA 1 cut(s) 111
Cac8I GCNNGC 2 cut(s) 113, 172
CfoI GCGC 4 cut(s) 126, 176, 238, 320
Cfr10I RCCGGY 1 cut(s) 241
Cfr13I GGNCC 4 cut(s) 144, 205, 206, 377
CseI GACGC 2 cut(s) 276, 338
Csp6I GTAC 1 cut(s) 61
CviAII CATG 2 cut(s) 277, 346
CviQI GTAC 1 cut(s) 61
DinI GGCGCC 1 cut(s) 319
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
DrdI GACNNNNNNGTC 2 cut(s) 188, 491
DseDI GACNNNNNNGTC 2 cut(s) 188, 491
EaeI YGGCCR 3 cut(s) 27, 99, 342
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 4 cut(s) 202, 267, 434, 504
Eco24I GRGCYC 2 cut(s) 117, 209
Eco47I GGWCC 2 cut(s) 144, 377
Eco47III AGCGCT 1 cut(s) 125
Eco88I CYCGRG 2 cut(s) 140, 230
EcoO109I RGGNCCY 2 cut(s) 144, 205
EcoRII CCWGG 1 cut(s) 215
EcoT38I GRGCYC 2 cut(s) 117, 209
EgeI GGCGCC 1 cut(s) 319
EheI GGCGCC 1 cut(s) 319
Esp3I CGTCTC 1 cut(s) 332
FaeI CATG 2 cut(s) 280, 349
FaiI YATR 2 cut(s) 278, 347
FatI CATG 2 cut(s) 276, 345
FauI CCCGC 4 cut(s) 179, 364, 406, 425
FblI GTMKAC 2 cut(s) 327, 450
FokI GGATG 2 cut(s) 88, 276
FriOI GRGCYC 2 cut(s) 117, 209
GlaI GCGC 4 cut(s) 125, 175, 237, 319
HaeII RGCGCY 3 cut(s) 127, 239, 321
HaeIII GGCC 5 cut(s) 29, 101, 207, 305, 344
HapII CCGG 3 cut(s) 130, 242, 473
HgaI GACGC 2 cut(s) 276, 338
HhaI GCGC 4 cut(s) 126, 176, 238, 320
Hin1I GRCGYC 2 cut(s) 287, 318
Hin1II CATG 2 cut(s) 280, 349
Hin6I GCGC 4 cut(s) 124, 174, 236, 318
HinP1I GCGC 4 cut(s) 124, 174, 236, 318
HincII GTYRAC 2 cut(s) 328, 451
HindII GTYRAC 2 cut(s) 328, 451
HinfI GANTC 3 cut(s) 106, 324, 492
HpaII CCGG 3 cut(s) 130, 242, 473
Hpy166II GTNNAC 2 cut(s) 328, 451
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 4 cut(s) 11, 110, 230, 337
Hpy8I GTNNAC 2 cut(s) 328, 451
Hpy99I CGWCG 5 cut(s) 164, 251, 272, 292, 332
HpyAV CCTTC 1 cut(s) 295
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 26, 121, 173, 258, 368, 416
Hsp92I GRCGYC 2 cut(s) 287, 318
Hsp92II CATG 2 cut(s) 280, 349
HspAI GCGC 4 cut(s) 124, 174, 236, 318
KasI GGCGCC 1 cut(s) 317
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 1 cut(s) 131
LpnPI CCDG 7 cut(s) 143, 202, 229, 255, 334, 343, 486
Lsp1109I GCAGC 4 cut(s) 4, 10, 225, 374
LweI GCATC 2 cut(s) 254, 453
MalI GATC 1 cut(s) 444
MbiI CCGCTC 1 cut(s) 432
MboI GATC 1 cut(s) 442
MboII GAAGA 3 cut(s) 49, 184, 310
MhlI GDGCHC 2 cut(s) 117, 209
MluCI AATT 2 cut(s) 223, 514
Mly113I GGCGCC 1 cut(s) 318
MlyI GAGTC 2 cut(s) 333, 501
MseI TTAA 1 cut(s) 517
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 3 cut(s) 130, 242, 473
MspR9I CCNGG 3 cut(s) 130, 217, 473
MvaI CCWGG 1 cut(s) 217
MvnI CGCG 3 cut(s) 111, 174, 332
MwoI GCNNNNNNNGC 7 cut(s) 23, 26, 121, 173, 258, 368, 416
NarI GGCGCC 1 cut(s) 318
NciI CCSGG 2 cut(s) 130, 473
NdeII GATC 1 cut(s) 442
NlaIII CATG 2 cut(s) 280, 349
NlaIV GGNNCC 5 cut(s) 145, 206, 207, 319, 378
NmeAIII GCCGAG 1 cut(s) 127
NruI TCGCGA 1 cut(s) 111
NspI RCATGY 1 cut(s) 280
PciI ACATGT 1 cut(s) 276
PcsI WCGNNNNNNNCGW 4 cut(s) 186, 287, 333, 492
PfeI GAWTC 1 cut(s) 106
PflFI GACNNNGTC 1 cut(s) 332
PfoI TCCNGGA 1 cut(s) 128
PleI GAGTC 2 cut(s) 332, 500
PluTI GGCGCC 1 cut(s) 321
PpsI GAGTC 2 cut(s) 332, 500
PpuMI RGGWCCY 1 cut(s) 144
PscI ACATGT 1 cut(s) 276
Psp5II RGGWCCY 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 215
PspGI CCWGG 1 cut(s) 215
PspN4I GGNNCC 5 cut(s) 145, 206, 207, 319, 378
PspOMI GGGCCC 1 cut(s) 205
PspPI GGNCC 4 cut(s) 144, 205, 206, 377
PspPPI RGGWCCY 1 cut(s) 144
PsyI GACNNNGTC 1 cut(s) 332
RruI TCGCGA 1 cut(s) 111
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SalI GTCGAC 2 cut(s) 326, 449
SaqAI TTAA 1 cut(s) 517
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 4 cut(s) 144, 205, 206, 377
SchI GAGTC 2 cut(s) 333, 501
ScrFI CCNGG 3 cut(s) 130, 217, 473
SduI GDGCHC 2 cut(s) 117, 209
SetI ASST 2 cut(s) 19, 315
SfaNI GCATC 2 cut(s) 254, 453
SfoI GGCGCC 1 cut(s) 319
SinI GGWCC 2 cut(s) 144, 377
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 2 cut(s) 223, 514
SspDI GGCGCC 1 cut(s) 317
StyD4I CCNGG 3 cut(s) 128, 215, 471
TaqI TCGA 4 cut(s) 290, 327, 336, 450
TasI AATT 2 cut(s) 223, 514
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 1 cut(s) 517
Tru9I TTAA 1 cut(s) 517
TseI GCWGC 4 cut(s) 17, 23, 238, 362
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 1 cut(s) 497
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 2 cut(s) 144, 377
XceI RCATGY 1 cut(s) 280
XmiI GTMKAC 2 cut(s) 327, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.