RLG00000017482

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014979
Physical Location & Seq
Reverse (-)
17421826 .. 17426615
4790 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017482

Sequence Viewer

Length: 1146 bp
ATGAACCACCACATGTTAAATAGGTGCGTGAAAACGATGAAACCAAAGGACCCGGCCCGACCCGGTTTCAGTTCACCTCGACGATCAGCAGCTCATCCGCACACCAACGTCGTTCGACTAGAAACCTGGTGCAGCACCGACCTCAGCGACTTCGACGCCGAGCCTCCACTACGGCGCGACGAAGAGAGAGAGGAGAGAGACAGCGCGGAGGAATCAGAATCTGGTTTGGAGTTTCCAAAGCTATCAGGCACGGAAGCTTGTCTCTGGCGCCACCAACAACGACGTCGGCCCCAACTCCATGGTCCTCACCTTCGCCAACCCTTTCTAGATCAAAATCTCCTTCCTCGCAAAACGGTCCATGAAAAACGTGAGACGAATGTAGGAGCTATTCGAAATCCGGCTCAGCGCAGTCATGGTCACCGAGTTCATCCTCGCCACCATGTTCTGGAACCCCTAAGAGACGTGGAGGCTTCCCCAGATGACAAGCTTAGTGGCCAAGTAGCATGCAACCCCTTCCTTCCCTCTCTTCCCAAAAACCCATTCACCTTGTTCAACCTATCTCCCTCTCTAATTCTTCCCCAATTCCCAGACCCACAACGGAACAACAAGTTTTTCTCTGCACTCATGGAGGGTCAAGAAGAAGCGGCTGCGGCTGCGGCTGCGGCCAATAGGAAGAGAGGCGAGAACGACAAGCCGTACAAGGGGATAAGGATGAGGAAGTGGGGCAAGTGGGTGGCCGAGATTCGCGAGCCCAACAAGCGCTCCCGGATTTGGCTCGGGTCGTACTCTACTCCCGTCGCCGCCGCCCGGGCCTACGACACCGCCGTCTTCTACTTGAGGGGCCCGTCCGCGAGGCTCAATTTTCCAGAGGCGCTGCCTGTCGTGGGCGGCGGCTGCGGCTGCGACGGCGACATGTCGGCGGCGTCGATAAGGAAGAAGGCCACCGAGGTCGGCGCCAGAGTCGACGCGGTCGAGACGGCCATGAGGCAACACAGGGCTGCGGCGGCGGGGGGTCCGATGGAGGAGATGAAGCCGTGTAGGGCGGAGGCGGAGGAGGAGAGCGGGTTCGTGGATCGGGTCGACTTGAACAAGATGCCCGACCCGGAGGAAACGGACGGCGGAGTTTGCGATTGGGGGAGGAATTAA

Protein Analysis

382

Amino Acids

42.72

Weight (kDa)

8.74

Isoelectric Point (pI)

59.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 233 - 281 2e-11 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23340 AT3G50260
fragaria_vesca FvH4_1g16370
malus_domestica MD02G1176200.v1.1 MD15G1286600.v1.1
prunus_persica Prupe.6G231500_v2.0.a1
pyrus_communis pycom02g14240 pycom15g24990
rosa_chinensis RchiOBHm_Chr2g0106241
rosa_laevigata RLG00000017482
rosa_multiflora Rmu_sc0008603.1_g000012
rosa_roxburghii Rroxscaffold_2G00137340
rosa_rugosa Rorug02G0133700
rosa_samantha Rh2AG184800 Rh2CG189900
rosa_wichuraiana Rw2G014430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 824
AatII GACGTC 1 cut(s) 286
AccB1I GGYRCC 2 cut(s) 267, 953
AccB7I CCANNNNNTGG 1 cut(s) 445
AccBSI CCGCTC 1 cut(s) 1062
AccI GTMKAC 2 cut(s) 963, 1080
AccII CGCG 5 cut(s) 177, 206, 747, 851, 968
AclWI GGATC 1 cut(s) 1080
AcoI YGGCCR 4 cut(s) 493, 663, 735, 978
AcyI GRCGYC 5 cut(s) 156, 268, 283, 923, 954
AfaI GTAC 2 cut(s) 698, 785
AfeI AGCGCT 1 cut(s) 761
AfiI CCNNNNNNNGG 5 cut(s) 445, 701, 771, 807, 884
AflIII ACRYGT 2 cut(s) 12, 912
AgsI TTSAA 2 cut(s) 553, 1087
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 1 cut(s) 125
AjuI GAANNNNNNNTTGG 2 cut(s) 524, 556
AloI GAACNNNNNNTCC 2 cut(s) 1049, 1081
AluBI AGCT 5 cut(s) 92, 241, 257, 386, 487
AluI AGCT 5 cut(s) 92, 241, 257, 386, 487
Alw26I GTCTC 5 cut(s) 192, 266, 365, 453, 968
AlwI GGATC 1 cut(s) 1080
AlwNI CAGNNNCTG 1 cut(s) 221
Ama87I CYCGRG 2 cut(s) 776, 807
Aor51HI AGCGCT 1 cut(s) 761
AoxI GGCC 9 cut(s) 54, 287, 493, 663, 735, 810, 841, 939, 978
ApaI GGGCCC 1 cut(s) 845
ApeKI GCWGC 9 cut(s) 89, 132, 647, 653, 659, 874, 894, 900, 998
AspLEI GCGC 7 cut(s) 177, 206, 270, 408, 762, 874, 956
AspS9I GGNCC 9 cut(s) 49, 55, 288, 302, 355, 810, 841, 842, 1013
AsuC2I CCSGG 6 cut(s) 53, 63, 766, 808, 809, 1103
AsuHPI GGTGA 4 cut(s) 66, 299, 410, 535
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 2 cut(s) 776, 807
AvaII GGWCC 4 cut(s) 49, 302, 355, 1013
BaeGI GKGCMC 1 cut(s) 845
BalI TGGCCA 1 cut(s) 495
BanI GGYRCC 2 cut(s) 267, 953
BanII GRGCYC 2 cut(s) 753, 845
BbsI GAAGAC 1 cut(s) 820
BbvCI CCTCAGC 1 cut(s) 143
BbvI GCAGC 9 cut(s) 101, 144, 634, 640, 646, 861, 881, 887, 985
BccI CCATC 1 cut(s) 1012
BceAI ACGGC 7 cut(s) 188, 679, 809, 922, 993, 1018, 1132
BciT130I CCWGG 1 cut(s) 127
BcnI CCSGG 6 cut(s) 53, 63, 766, 808, 809, 1103
BcoDI GTCTC 5 cut(s) 192, 266, 365, 453, 968
BfaI CTAG 2 cut(s) 119, 326
BfoI RGCGCY 4 cut(s) 271, 763, 875, 957
BglI GCCNNNNNGGC 1 cut(s) 809
BlpI GCTNAGC 1 cut(s) 402
Bme1390I CCNGG 7 cut(s) 53, 63, 127, 766, 808, 809, 1103
Bme18I GGWCC 4 cut(s) 49, 302, 355, 1013
BmeT110I CYCGRG 2 cut(s) 776, 807
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 9 cut(s) 49, 55, 288, 302, 355, 810, 841, 842, 1013
BmiI GGNNCC 8 cut(s) 51, 269, 290, 450, 842, 843, 955, 1014
BmrFI CCNGG 7 cut(s) 53, 63, 127, 766, 808, 809, 1103
BmsI GCATC 1 cut(s) 1083
BpiI GAAGAC 1 cut(s) 820
Bpu10I CCTNAGC 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 402
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 1 cut(s) 856
BpuMI CCSGG 6 cut(s) 53, 63, 766, 808, 809, 1103
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 5 cut(s) 156, 268, 283, 923, 954
BsaJI CCNNGG 3 cut(s) 298, 807, 945
BsaXI ACNNNNNCTCC 4 cut(s) 746, 776, 1049, 1079
Bsc4I CCNNNNNNNGG 5 cut(s) 445, 701, 771, 807, 884
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 1 cut(s) 127
BseDI CCNNGG 3 cut(s) 298, 807, 945
BseGI GGATG 3 cut(s) 94, 427, 717
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 5 cut(s) 445, 701, 771, 807, 884
BseMII CTCAG 2 cut(s) 157, 416
BseRI GAGGAG 4 cut(s) 206, 1037, 1067, 1070
BseSI GKGCMC 1 cut(s) 845
BseXI GCAGC 9 cut(s) 101, 144, 634, 640, 646, 861, 881, 887, 985
BsgI GTGCAG 2 cut(s) 151, 603
Bsh1236I CGCG 5 cut(s) 177, 206, 747, 851, 968
Bsh1285I CGRYCG 1 cut(s) 972
BshFI GGCC 9 cut(s) 56, 289, 495, 665, 737, 812, 843, 941, 980
BshNI GGYRCC 2 cut(s) 267, 953
BsiEI CGRYCG 1 cut(s) 972
BsiHKCI CYCGRG 2 cut(s) 776, 807
BsiSI CCGG 6 cut(s) 53, 63, 398, 766, 808, 1103
BslI CCNNNNNNNGG 5 cut(s) 445, 701, 771, 807, 884
BsmAI GTCTC 5 cut(s) 192, 266, 365, 453, 968
BsmBI CGTCTC 3 cut(s) 365, 453, 968
BsnI GGCC 9 cut(s) 56, 289, 495, 665, 737, 812, 843, 941, 980
BsoBI CYCGRG 2 cut(s) 776, 807
Bsp119I TTCGAA 1 cut(s) 391
Bsp120I GGGCCC 1 cut(s) 841
Bsp1286I GDGCHC 2 cut(s) 753, 845
Bsp143I GATC 3 cut(s) 83, 328, 1072
Bsp1720I GCTNAGC 1 cut(s) 402
Bsp19I CCATGG 1 cut(s) 298
Bsp68I TCGCGA 1 cut(s) 747
BspANI GGCC 9 cut(s) 56, 289, 495, 665, 737, 812, 843, 941, 980
BspCNI CTCAG 2 cut(s) 156, 415
BspFNI CGCG 5 cut(s) 177, 206, 747, 851, 968
BspLI GGNNCC 8 cut(s) 51, 269, 290, 450, 842, 843, 955, 1014
BspPI GGATC 1 cut(s) 1080
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 2 cut(s) 267, 953
BsrBI CCGCTC 1 cut(s) 1062
BssECI CCNNGG 3 cut(s) 298, 807, 945
BssMI GATC 3 cut(s) 83, 328, 1072
BssNI GRCGYC 5 cut(s) 156, 268, 283, 923, 954
BssT1I CCWWGG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 127
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 3 cut(s) 177, 531, 668
BstACI GRCGYC 5 cut(s) 156, 268, 283, 923, 954
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 2 cut(s) 505, 749
BstDEI CTNAG 4 cut(s) 143, 402, 455, 488
BstDSI CCRYGG 1 cut(s) 298
BstEII GGTNACC 1 cut(s) 416
BstF5I GGATG 3 cut(s) 94, 427, 717
BstFNI CGCG 5 cut(s) 177, 206, 747, 851, 968
BstH2I RGCGCY 4 cut(s) 271, 763, 875, 957
BstHHI GCGC 7 cut(s) 177, 206, 270, 408, 762, 874, 956
BstKTI GATC 3 cut(s) 86, 331, 1075
BstMAI GTCTC 5 cut(s) 192, 266, 365, 453, 968
BstMBI GATC 3 cut(s) 83, 328, 1072
BstMCI CGRYCG 1 cut(s) 972
BstNI CCWGG 1 cut(s) 127
BstNSI RCATGY 3 cut(s) 16, 507, 916
BstPI GGTNACC 1 cut(s) 416
BstSCI CCNGG 7 cut(s) 51, 61, 125, 764, 806, 807, 1101
BstSLI GKGCMC 1 cut(s) 845
BstUI CGCG 5 cut(s) 177, 206, 747, 851, 968
BstV1I GCAGC 9 cut(s) 101, 144, 634, 640, 646, 861, 881, 887, 985
BstV2I GAAGAC 1 cut(s) 820
BstXI CCANNNNNNTGG 1 cut(s) 299
BsuRI GGCC 9 cut(s) 56, 289, 495, 665, 737, 812, 843, 941, 980
BtgI CCRYGG 1 cut(s) 298
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 3 cut(s) 94, 427, 717
BtuMI TCGCGA 1 cut(s) 747
Cac8I GCNNGC 2 cut(s) 505, 749
CaiI CAGNNNCTG 1 cut(s) 221
CfoI GCGC 7 cut(s) 177, 206, 270, 408, 762, 874, 956
Cfr13I GGNCC 9 cut(s) 49, 55, 288, 302, 355, 810, 841, 842, 1013
Cfr9I CCCGGG 1 cut(s) 807
CseI GACGC 3 cut(s) 164, 912, 974
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 2 cut(s) 697, 784
CspCI CAANNNNNGTGG 2 cut(s) 472, 507
CviAII CATG 9 cut(s) 13, 299, 359, 413, 440, 504, 625, 913, 982
CviQI GTAC 2 cut(s) 697, 784
DdeI CTNAG 4 cut(s) 143, 402, 455, 488
DinI GGCGCC 2 cut(s) 269, 955
DpnI GATC 3 cut(s) 85, 330, 1074
DpnII GATC 3 cut(s) 83, 328, 1072
DrdI GACNNNNNNGTC 1 cut(s) 824
DseDI GACNNNNNNGTC 1 cut(s) 824
EaeI YGGCCR 4 cut(s) 493, 663, 735, 978
Eam1104I CTCTTC 3 cut(s) 177, 531, 668
EarI CTCTTC 3 cut(s) 177, 531, 668
EciI GGCGGA 3 cut(s) 1058, 1064, 1134
Eco130I CCWWGG 1 cut(s) 298
Eco24I GRGCYC 2 cut(s) 753, 845
Eco47I GGWCC 4 cut(s) 49, 302, 355, 1013
Eco47III AGCGCT 1 cut(s) 761
Eco88I CYCGRG 2 cut(s) 776, 807
Eco91I GGTNACC 1 cut(s) 416
EcoO109I RGGNCCY 2 cut(s) 49, 841
EcoO65I GGTNACC 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 298
EcoT38I GRGCYC 2 cut(s) 753, 845
EgeI GGCGCC 2 cut(s) 269, 955
EheI GGCGCC 2 cut(s) 269, 955
ErhI CCWWGG 1 cut(s) 298
Esp3I CGTCTC 3 cut(s) 365, 453, 968
FaeI CATG 9 cut(s) 16, 302, 362, 416, 443, 507, 628, 916, 985
FaiI YATR 9 cut(s) 14, 300, 360, 414, 441, 505, 626, 914, 983
FatI CATG 9 cut(s) 12, 298, 358, 412, 439, 503, 624, 912, 981
FauI CCCGC 2 cut(s) 1000, 1055
FblI GTMKAC 2 cut(s) 963, 1080
FokI GGATG 3 cut(s) 81, 414, 724
FriOI GRGCYC 2 cut(s) 753, 845
FspBI CTAG 2 cut(s) 119, 326
GlaI GCGC 7 cut(s) 176, 205, 269, 407, 761, 873, 955
HaeII RGCGCY 4 cut(s) 271, 763, 875, 957
HaeIII GGCC 9 cut(s) 56, 289, 495, 665, 737, 812, 843, 941, 980
HapII CCGG 6 cut(s) 53, 63, 398, 766, 808, 1103
HgaI GACGC 3 cut(s) 164, 912, 974
HhaI GCGC 7 cut(s) 177, 206, 270, 408, 762, 874, 956
Hin1I GRCGYC 5 cut(s) 156, 268, 283, 923, 954
Hin1II CATG 9 cut(s) 16, 302, 362, 416, 443, 507, 628, 916, 985
Hin6I GCGC 7 cut(s) 175, 204, 268, 406, 760, 872, 954
HinP1I GCGC 7 cut(s) 175, 204, 268, 406, 760, 872, 954
HincII GTYRAC 2 cut(s) 964, 1081
HindII GTYRAC 2 cut(s) 964, 1081
HindIII AAGCTT 2 cut(s) 255, 485
HinfI GANTC 4 cut(s) 212, 218, 742, 960
HpaII CCGG 6 cut(s) 53, 63, 398, 766, 808, 1103
HphI GGTGA 4 cut(s) 66, 299, 410, 535
Hpy166II GTNNAC 3 cut(s) 74, 964, 1081
Hpy188I TCNGA 2 cut(s) 217, 1017
Hpy188III TCNNGA 6 cut(s) 326, 446, 635, 746, 866, 973
Hpy8I GTNNAC 3 cut(s) 74, 964, 1081
HpyAV CCTTC 5 cut(s) 320, 350, 523, 527, 931
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 4 cut(s) 108, 283, 367, 462
HpyCH4V TGCA 3 cut(s) 132, 507, 620
HpyF3I CTNAG 4 cut(s) 143, 402, 455, 488
HpySE526I ACGT 4 cut(s) 108, 283, 367, 462
Hsp92I GRCGYC 5 cut(s) 156, 268, 283, 923, 954
Hsp92II CATG 9 cut(s) 16, 302, 362, 416, 443, 507, 628, 916, 985
HspAI GCGC 7 cut(s) 175, 204, 268, 406, 760, 872, 954
KasI GGCGCC 2 cut(s) 267, 953
Kzo9I GATC 3 cut(s) 83, 328, 1072
LmnI GCTCC 2 cut(s) 383, 767
Lsp1109I GCAGC 9 cut(s) 101, 144, 634, 640, 646, 861, 881, 887, 985
LweI GCATC 1 cut(s) 1083
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 2 cut(s) 119, 326
MaeII ACGT 4 cut(s) 108, 283, 367, 462
MaeIII GTNAC 1 cut(s) 416
MalI GATC 3 cut(s) 85, 330, 1074
MbiI CCGCTC 1 cut(s) 1062
MboI GATC 3 cut(s) 83, 328, 1072
MboII GAAGA 7 cut(s) 194, 518, 566, 650, 685, 820, 946
MhlI GDGCHC 2 cut(s) 753, 845
MlsI TGGCCA 1 cut(s) 495
MluCI AATT 4 cut(s) 570, 581, 859, 1141
MluNI TGGCCA 1 cut(s) 495
Mly113I GGCGCC 2 cut(s) 268, 954
MlyI GAGTC 1 cut(s) 969
Mox20I TGGCCA 1 cut(s) 495
MscI TGGCCA 1 cut(s) 495
MseI TTAA 2 cut(s) 17, 1144
Msp20I TGGCCA 1 cut(s) 495
MspI CCGG 6 cut(s) 53, 63, 398, 766, 808, 1103
MspR9I CCNGG 7 cut(s) 53, 63, 127, 766, 808, 809, 1103
MvaI CCWGG 1 cut(s) 127
MvnI CGCG 5 cut(s) 177, 206, 747, 851, 968
NarI GGCGCC 2 cut(s) 268, 954
NciI CCSGG 6 cut(s) 53, 63, 766, 808, 809, 1103
NcoI CCATGG 1 cut(s) 298
NdeII GATC 3 cut(s) 83, 328, 1072
NlaIII CATG 9 cut(s) 16, 302, 362, 416, 443, 507, 628, 916, 985
NlaIV GGNNCC 8 cut(s) 51, 269, 290, 450, 842, 843, 955, 1014
NmeAIII GCCGAG 2 cut(s) 184, 763
NmuCI GTSAC 1 cut(s) 416
NruI TCGCGA 1 cut(s) 747
NspI RCATGY 3 cut(s) 16, 507, 916
NspV TTCGAA 1 cut(s) 391
PaeI GCATGC 1 cut(s) 507
PciI ACATGT 2 cut(s) 12, 912
PcsI WCGNNNNNNNCGW 3 cut(s) 822, 923, 969
PfeI GAWTC 3 cut(s) 212, 218, 742
PflFI GACNNNGTC 1 cut(s) 968
PflMI CCANNNNNTGG 1 cut(s) 445
PfoI TCCNGGA 1 cut(s) 764
PleI GAGTC 1 cut(s) 968
PluTI GGCGCC 2 cut(s) 271, 957
PpsI GAGTC 1 cut(s) 968
PpuMI RGGWCCY 1 cut(s) 49
PscI ACATGT 2 cut(s) 12, 912
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 1 cut(s) 125
PspEI GGTNACC 1 cut(s) 416
PspGI CCWGG 1 cut(s) 125
PspN4I GGNNCC 8 cut(s) 51, 269, 290, 450, 842, 843, 955, 1014
PspOMI GGGCCC 1 cut(s) 841
PspPI GGNCC 9 cut(s) 49, 55, 288, 302, 355, 810, 841, 842, 1013
PspPPI RGGWCCY 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 221
PsyI GACNNNGTC 1 cut(s) 968
RruI TCGCGA 1 cut(s) 747
RsaI GTAC 2 cut(s) 698, 785
RsaNI GTAC 2 cut(s) 697, 784
SalI GTCGAC 2 cut(s) 962, 1079
SaqAI TTAA 2 cut(s) 17, 1144
Sau3AI GATC 3 cut(s) 83, 328, 1072
Sau96I GGNCC 9 cut(s) 49, 55, 288, 302, 355, 810, 841, 842, 1013
SchI GAGTC 1 cut(s) 969
ScrFI CCNGG 7 cut(s) 53, 63, 127, 766, 808, 809, 1103
SduI GDGCHC 2 cut(s) 753, 845
SexAI ACCWGGT 1 cut(s) 125
SfaNI GCATC 1 cut(s) 1083
SfoI GGCGCC 2 cut(s) 269, 955
SfuI TTCGAA 1 cut(s) 391
SinI GGWCC 4 cut(s) 49, 302, 355, 1013
SmaI CCCGGG 1 cut(s) 809
SmlI CTYRAG 1 cut(s) 835
SmoI CTYRAG 1 cut(s) 835
SphI GCATGC 1 cut(s) 507
SrfI GCCCGGGC 1 cut(s) 809
Sse9I AATT 4 cut(s) 570, 581, 859, 1141
SspDI GGCGCC 2 cut(s) 267, 953
SspMI CTAG 2 cut(s) 119, 326
StyD4I CCNGG 7 cut(s) 51, 61, 125, 764, 806, 807, 1101
StyI CCWWGG 1 cut(s) 298
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 4 cut(s) 111, 286, 370, 465
TaqI TCGA 8 cut(s) 79, 115, 153, 391, 926, 963, 972, 1080
TasI AATT 4 cut(s) 570, 581, 859, 1141
TfiI GAWTC 3 cut(s) 212, 218, 742
Tru1I TTAA 2 cut(s) 17, 1144
Tru9I TTAA 2 cut(s) 17, 1144
TseFI GTSAC 1 cut(s) 416
TseI GCWGC 9 cut(s) 89, 132, 647, 653, 659, 874, 894, 900, 998
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 5 cut(s) 17, 53, 375, 416, 1043
TspGWI ACGGA 3 cut(s) 266, 613, 1127
TspMI CCCGGG 1 cut(s) 807
Tth111I GACNNNGTC 1 cut(s) 968
Van91I CCANNNNNTGG 1 cut(s) 445
VpaK11BI GGWCC 4 cut(s) 49, 302, 355, 1013
XbaI TCTAGA 1 cut(s) 325
XceI RCATGY 3 cut(s) 16, 507, 916
XmaI CCCGGG 1 cut(s) 807
XmiI GTMKAC 2 cut(s) 963, 1080
XspI CTAG 2 cut(s) 119, 326
ZraI GACGTC 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.