Rh2AG184800

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014979
Physical Location & Seq
Reverse (-)
18071482 .. 18072006
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG184800.1

Sequence Viewer

Length: 525 bp
ATGGAGGGTCAAGAAGCTGCGGCCGCGGCCAATAGGAAGAGAGGCGAGAACGACAAGCCGTACAAGGGGATAAGGATGAGGAAGTGGGGCAAGTGGGTGGCCGAGATTCGCGAGCCCAACAAGCGCTCCCGGATTTGGCTCGGGTCGTACTCGACTCCCGTCGCCGCCGCCCGCGCCTACGACACCGCCGTCTTCTACTTGAGGGGCCCGTCCGCGAGGCTCAATTTTCCCGAGGCCCTTCCGGTCGTCGGCGGAGGGTGCGGATGCGACGGCGACATGTCGGCGGCGTCGATAAGGAAGAAGGCCACCGAGGTCGGCGCCAGAGTCGACGCGGTCGAGACGGCCATGCGGCAACACAGGGCGGCTGCGGCGGGGGGTCCGATGGAGGAGATGAAGCCGTGTAGGGCGGCGGCGGCGGAGGAGGAGGAGAGCGGGTTCGTGGATCGGGTCGACTTGAACAAGATGCCCGACCCGGAGGAAACGGACGGCGGCGGCGGAGTCGTCTGCGATTGGGGGAGGAATTAA

Protein Analysis

174

Amino Acids

18.77

Weight (kDa)

7.63

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 21 - 69 5.5e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23340 AT3G50260
fragaria_vesca FvH4_1g16370
malus_domestica MD02G1176200.v1.1 MD15G1286600.v1.1
prunus_persica Prupe.6G231500_v2.0.a1
pyrus_communis pycom02g14240 pycom15g24990
rosa_chinensis RchiOBHm_Chr2g0106241
rosa_laevigata RLG00000017482
rosa_multiflora Rmu_sc0008603.1_g000012
rosa_roxburghii Rroxscaffold_2G00137340
rosa_rugosa Rorug02G0133700
rosa_samantha Rh2AG184800 Rh2CG189900
rosa_wichuraiana Rw2G014430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 317
AccBSI CCGCTC 1 cut(s) 432
AccI GTMKAC 2 cut(s) 327, 450
AccII CGCG 5 cut(s) 26, 111, 174, 215, 332
AclWI GGATC 1 cut(s) 450
AcoI YGGCCR 4 cut(s) 21, 27, 99, 342
AcyI GRCGYC 2 cut(s) 287, 318
AfaI GTAC 2 cut(s) 62, 149
AfeI AGCGCT 1 cut(s) 125
AfiI CCNNNNNNNGG 3 cut(s) 65, 135, 248
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 1 cut(s) 457
AloI GAACNNNNNNTCC 2 cut(s) 419, 451
AluBI AGCT 1 cut(s) 17
AluI AGCT 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 1 cut(s) 450
Ama87I CYCGRG 2 cut(s) 140, 230
Aor51HI AGCGCT 1 cut(s) 125
AoxI GGCC 7 cut(s) 21, 27, 99, 205, 234, 303, 342
ApaI GGGCCC 1 cut(s) 209
ApeKI GCWGC 2 cut(s) 17, 365
AspLEI GCGC 3 cut(s) 126, 176, 320
AspS9I GGNCC 4 cut(s) 205, 206, 235, 377
AsuC2I CCSGG 2 cut(s) 130, 473
AvaI CYCGRG 2 cut(s) 140, 230
AvaII GGWCC 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 209
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 2 cut(s) 117, 209
BbsI GAAGAC 1 cut(s) 184
BbvI GCAGC 2 cut(s) 4, 352
BccI CCATC 1 cut(s) 376
BceAI ACGGC 6 cut(s) 43, 173, 286, 357, 382, 502
BcnI CCSGG 2 cut(s) 130, 473
BcoDI GTCTC 1 cut(s) 332
BfoI RGCGCY 2 cut(s) 127, 321
Bme1390I CCNGG 2 cut(s) 130, 473
Bme18I GGWCC 1 cut(s) 377
BmeT110I CYCGRG 2 cut(s) 140, 230
BmgT120I GGNCC 4 cut(s) 205, 206, 235, 377
BmiI GGNNCC 4 cut(s) 206, 207, 319, 378
BmrFI CCNGG 2 cut(s) 130, 473
BmsI GCATC 2 cut(s) 254, 453
BoxI GACNNNNGTC 1 cut(s) 158
BpiI GAAGAC 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 220
BpuMI CCSGG 2 cut(s) 130, 473
BsaHI GRCGYC 2 cut(s) 287, 318
BsaJI CCNNGG 3 cut(s) 24, 231, 309
BsaWI WCCGGW 1 cut(s) 241
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 419, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 135, 248
BseDI CCNNGG 3 cut(s) 24, 231, 309
BseGI GGATG 2 cut(s) 81, 269
BseLI CCNNNNNNNGG 3 cut(s) 65, 135, 248
BseRI GAGGAG 4 cut(s) 401, 434, 437, 440
BseSI GKGCMC 1 cut(s) 209
BseX3I CGGCCG 1 cut(s) 21
BseXI GCAGC 2 cut(s) 4, 352
Bsh1236I CGCG 5 cut(s) 26, 111, 174, 215, 332
Bsh1285I CGRYCG 3 cut(s) 24, 246, 336
BshFI GGCC 7 cut(s) 23, 29, 101, 207, 236, 305, 344
BshNI GGYRCC 1 cut(s) 317
BsiEI CGRYCG 3 cut(s) 24, 246, 336
BsiHKCI CYCGRG 2 cut(s) 140, 230
BsiSI CCGG 3 cut(s) 130, 242, 473
BslI CCNNNNNNNGG 3 cut(s) 65, 135, 248
BsmAI GTCTC 1 cut(s) 332
BsmBI CGTCTC 1 cut(s) 332
BsnI GGCC 7 cut(s) 23, 29, 101, 207, 236, 305, 344
BsoBI CYCGRG 2 cut(s) 140, 230
Bsp120I GGGCCC 1 cut(s) 205
Bsp1286I GDGCHC 2 cut(s) 117, 209
Bsp143I GATC 1 cut(s) 442
Bsp68I TCGCGA 1 cut(s) 111
BspANI GGCC 7 cut(s) 23, 29, 101, 207, 236, 305, 344
BspFNI CGCG 5 cut(s) 26, 111, 174, 215, 332
BspLI GGNNCC 4 cut(s) 206, 207, 319, 378
BspPI GGATC 1 cut(s) 450
BspT107I GGYRCC 1 cut(s) 317
BsrBI CCGCTC 1 cut(s) 432
BssECI CCNNGG 3 cut(s) 24, 231, 309
BssMI GATC 1 cut(s) 442
BssNI GRCGYC 2 cut(s) 287, 318
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 2 cut(s) 287, 318
BstC8I GCNNGC 2 cut(s) 113, 172
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 81, 269
BstFNI CGCG 5 cut(s) 26, 111, 174, 215, 332
BstH2I RGCGCY 2 cut(s) 127, 321
BstHHI GCGC 3 cut(s) 126, 176, 320
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 1 cut(s) 442
BstMCI CGRYCG 3 cut(s) 24, 246, 336
BstMWI GCNNNNNNNGC 7 cut(s) 23, 26, 121, 173, 258, 368, 413
BstNSI RCATGY 1 cut(s) 280
BstPAI GACNNNNGTC 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 128, 471
BstSLI GKGCMC 1 cut(s) 209
BstUI CGCG 5 cut(s) 26, 111, 174, 215, 332
BstV1I GCAGC 2 cut(s) 4, 352
BstV2I GAAGAC 1 cut(s) 184
BstZI CGGCCG 1 cut(s) 21
BsuRI GGCC 7 cut(s) 23, 29, 101, 207, 236, 305, 344
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 2 cut(s) 81, 269
BtuMI TCGCGA 1 cut(s) 111
Cac8I GCNNGC 2 cut(s) 113, 172
CciNI GCGGCCGC 1 cut(s) 21
CfoI GCGC 3 cut(s) 126, 176, 320
Cfr13I GGNCC 4 cut(s) 205, 206, 235, 377
Cfr42I CCGCGG 1 cut(s) 27
CseI GACGC 2 cut(s) 276, 338
Csp6I GTAC 2 cut(s) 61, 148
CviAII CATG 2 cut(s) 277, 346
CviQI GTAC 2 cut(s) 61, 148
DinI GGCGCC 1 cut(s) 319
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
EaeI YGGCCR 4 cut(s) 21, 27, 99, 342
EagI CGGCCG 1 cut(s) 21
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 3 cut(s) 267, 431, 510
EclXI CGGCCG 1 cut(s) 21
Eco24I GRGCYC 2 cut(s) 117, 209
Eco47I GGWCC 1 cut(s) 377
Eco47III AGCGCT 1 cut(s) 125
Eco52I CGGCCG 1 cut(s) 21
Eco88I CYCGRG 2 cut(s) 140, 230
EcoO109I RGGNCCY 2 cut(s) 205, 235
EcoT38I GRGCYC 2 cut(s) 117, 209
EgeI GGCGCC 1 cut(s) 319
EheI GGCGCC 1 cut(s) 319
Esp3I CGTCTC 1 cut(s) 332
FaeI CATG 2 cut(s) 280, 349
FaiI YATR 2 cut(s) 278, 347
FatI CATG 2 cut(s) 276, 345
FauI CCCGC 3 cut(s) 179, 364, 425
FblI GTMKAC 2 cut(s) 327, 450
FokI GGATG 2 cut(s) 88, 276
FriOI GRGCYC 2 cut(s) 117, 209
GlaI GCGC 3 cut(s) 125, 175, 319
HaeII RGCGCY 2 cut(s) 127, 321
HaeIII GGCC 7 cut(s) 23, 29, 101, 207, 236, 305, 344
HapII CCGG 3 cut(s) 130, 242, 473
HgaI GACGC 2 cut(s) 276, 338
HhaI GCGC 3 cut(s) 126, 176, 320
Hin1I GRCGYC 2 cut(s) 287, 318
Hin1II CATG 2 cut(s) 280, 349
Hin6I GCGC 3 cut(s) 124, 174, 318
HinP1I GCGC 3 cut(s) 124, 174, 318
HincII GTYRAC 2 cut(s) 328, 451
HindII GTYRAC 2 cut(s) 328, 451
HinfI GANTC 4 cut(s) 106, 154, 324, 498
HpaII CCGG 3 cut(s) 130, 242, 473
Hpy166II GTNNAC 2 cut(s) 328, 451
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 4 cut(s) 11, 110, 230, 337
Hpy8I GTNNAC 2 cut(s) 328, 451
Hpy99I CGWCG 5 cut(s) 164, 251, 272, 292, 332
HpyAV CCTTC 2 cut(s) 248, 295
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 26, 121, 173, 258, 368, 413
Hsp92I GRCGYC 2 cut(s) 287, 318
Hsp92II CATG 2 cut(s) 280, 349
HspAI GCGC 3 cut(s) 124, 174, 318
KasI GGCGCC 1 cut(s) 317
KspI CCGCGG 1 cut(s) 27
Kzo9I GATC 1 cut(s) 442
LmnI GCTCC 1 cut(s) 131
LpnPI CCDG 5 cut(s) 143, 255, 334, 343, 486
Lsp1109I GCAGC 2 cut(s) 4, 352
LweI GCATC 2 cut(s) 254, 453
MalI GATC 1 cut(s) 444
MbiI CCGCTC 1 cut(s) 432
MboI GATC 1 cut(s) 442
MboII GAAGA 3 cut(s) 49, 184, 310
MhlI GDGCHC 2 cut(s) 117, 209
MluCI AATT 2 cut(s) 223, 520
Mly113I GGCGCC 1 cut(s) 318
MlyI GAGTC 3 cut(s) 148, 333, 507
MseI TTAA 1 cut(s) 523
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 3 cut(s) 130, 242, 473
MspR9I CCNGG 2 cut(s) 130, 473
MvnI CGCG 5 cut(s) 26, 111, 174, 215, 332
MwoI GCNNNNNNNGC 7 cut(s) 23, 26, 121, 173, 258, 368, 413
NarI GGCGCC 1 cut(s) 318
NciI CCSGG 2 cut(s) 130, 473
NdeII GATC 1 cut(s) 442
NlaIII CATG 2 cut(s) 280, 349
NlaIV GGNNCC 4 cut(s) 206, 207, 319, 378
NmeAIII GCCGAG 1 cut(s) 127
NotI GCGGCCGC 1 cut(s) 21
NruI TCGCGA 1 cut(s) 111
NspI RCATGY 1 cut(s) 280
PciI ACATGT 1 cut(s) 276
PcsI WCGNNNNNNNCGW 3 cut(s) 186, 287, 333
PfeI GAWTC 1 cut(s) 106
PflFI GACNNNGTC 1 cut(s) 332
PfoI TCCNGGA 1 cut(s) 128
PleI GAGTC 3 cut(s) 148, 332, 506
PluTI GGCGCC 1 cut(s) 321
PpsI GAGTC 3 cut(s) 148, 332, 506
PscI ACATGT 1 cut(s) 276
PshAI GACNNNNGTC 1 cut(s) 158
PspN4I GGNNCC 4 cut(s) 206, 207, 319, 378
PspOMI GGGCCC 1 cut(s) 205
PspPI GGNCC 4 cut(s) 205, 206, 235, 377
PsyI GACNNNGTC 1 cut(s) 332
RruI TCGCGA 1 cut(s) 111
RsaI GTAC 2 cut(s) 62, 149
RsaNI GTAC 2 cut(s) 61, 148
SacII CCGCGG 1 cut(s) 27
SalI GTCGAC 2 cut(s) 326, 449
SaqAI TTAA 1 cut(s) 523
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 4 cut(s) 205, 206, 235, 377
SchI GAGTC 3 cut(s) 148, 333, 507
ScrFI CCNGG 2 cut(s) 130, 473
SduI GDGCHC 2 cut(s) 117, 209
SetI ASST 2 cut(s) 19, 315
SfaNI GCATC 2 cut(s) 254, 453
SfoI GGCGCC 1 cut(s) 319
Sfr303I CCGCGG 1 cut(s) 27
SgrBI CCGCGG 1 cut(s) 27
SinI GGWCC 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 2 cut(s) 223, 520
SspDI GGCGCC 1 cut(s) 317
StyD4I CCNGG 2 cut(s) 128, 471
TaqI TCGA 5 cut(s) 152, 290, 327, 336, 450
TasI AATT 2 cut(s) 223, 520
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 1 cut(s) 523
Tru9I TTAA 1 cut(s) 523
TseI GCWGC 2 cut(s) 17, 365
TspDTI ATGAA 1 cut(s) 407
TspGWI ACGGA 1 cut(s) 497
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 377
XceI RCATGY 1 cut(s) 280
XmiI GTMKAC 2 cut(s) 327, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.