pycom15g24990

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014979
Physical Location & Seq
Reverse (-)
19340934 .. 19341842
909 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g24990.1

Sequence Viewer

Length: 720 bp
ATGAAATTATCTATCCTAAGGGAGAGGAGGTTGTCACTTTTAACGAGAATCGAACCTAAAACCTCTCGTTTTCTTTTGTTGGGTTTCAGAGACGTGGAAGCCTACCCAGACGACAACCTTAGTGGCCAAGTAGCATGCAAAACCCCTCCTCCCACCTCTCTCCCTCTCTCCCTCAGAAAGCAGAGCCCTCCTTTTGCACTCTCAGACCGAACAACAATTGAAGGAGAAGAATTGAAGAGGAAGAAGATGGAGTGCAGCGGAGACACGGCGAACAGGAAGAGAGCGGTGGAGAACGACAAGCCGTACAAGGGGATAAGGATGAGGAAGTGGGGAAAGTGGGTGGCCGAGATTCGAGAACCCAACAAGCGCTCCAGAATTTGGCTCGGCTCCTACTCCACTCCCGTCGCCGCCGCCCGGGCGTACGATACGGCTGTTTTTTACTTGAGGGGTCCATCGGCGCGGCTCAATTTTCCAGAGGCTTTGGCGGTCGAAGGCGGCGGCTGCGGGTGCGGCGACATGTCGGCGGCCTCAATACGCAAACGGGCCACCGAGGTCGGTGCCCGGGTCGACGCGGTGGAGACCGCTATGCGAAACCACCGCGGTTCGGCCGGGGGGAACGGAGACGGGGCTGAGATTAAGCCTTGCGTCGCCGGATTCGTGGATCGGGTCGACTTGAACAAGATGCCCGACCCGGAGAACTCGGAAGGTGAGTGGAAATAG

Protein Analysis

240

Amino Acids

26.41

Weight (kDa)

9.57

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 102 - 150 9.8e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014979)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23340 AT3G50260
fragaria_vesca FvH4_1g16370
malus_domestica MD02G1176200.v1.1 MD15G1286600.v1.1
prunus_persica Prupe.6G231500_v2.0.a1
pyrus_communis pycom02g14240 pycom15g24990
rosa_chinensis RchiOBHm_Chr2g0106241
rosa_laevigata RLG00000017482
rosa_multiflora Rmu_sc0008603.1_g000012
rosa_roxburghii Rroxscaffold_2G00137340
rosa_rugosa Rorug02G0133700
rosa_samantha Rh2AG184800 Rh2CG189900
rosa_wichuraiana Rw2G014430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 557
AccB7I CCANNNNNTGG 1 cut(s) 378
AccBSI CCGCTC 1 cut(s) 284
AccI GTMKAC 2 cut(s) 567, 669
AccII CGCG 3 cut(s) 460, 572, 600
AclWI GGATC 1 cut(s) 669
AcoI YGGCCR 3 cut(s) 124, 342, 606
AcsI RAATTY 1 cut(s) 375
AfaI GTAC 2 cut(s) 305, 422
AfeI AGCGCT 1 cut(s) 368
AfiI CCNNNNNNNGG 4 cut(s) 308, 378, 414, 604
AflIII ACRYGT 1 cut(s) 516
AgsI TTSAA 3 cut(s) 221, 235, 676
AjiI CACGTC 1 cut(s) 94
Alw26I GTCTC 4 cut(s) 84, 255, 572, 615
AlwI GGATC 1 cut(s) 669
Ama87I CYCGRG 2 cut(s) 414, 561
Aor51HI AGCGCT 1 cut(s) 368
AoxI GGCC 5 cut(s) 124, 342, 525, 543, 606
ApeKI GCWGC 2 cut(s) 255, 501
ApoI RAATTY 1 cut(s) 375
AspLEI GCGC 2 cut(s) 369, 460
AspS9I GGNCC 2 cut(s) 449, 543
AsuC2I CCSGG 6 cut(s) 415, 416, 562, 563, 610, 692
AsuHPI GGTGA 1 cut(s) 719
AvaI CYCGRG 2 cut(s) 414, 561
AvaII GGWCC 1 cut(s) 449
AxyI CCTNAGG 1 cut(s) 17
BaeGI GKGCMC 1 cut(s) 562
BaeI ACNNNNGTAYC 2 cut(s) 417, 450
BalI TGGCCA 1 cut(s) 126
BanI GGYRCC 1 cut(s) 557
BanII GRGCYC 1 cut(s) 188
BbvI GCAGC 2 cut(s) 267, 488
BccI CCATC 2 cut(s) 241, 460
BceAI ACGGC 3 cut(s) 282, 286, 444
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BcnI CCSGG 6 cut(s) 415, 416, 562, 563, 610, 692
BcoDI GTCTC 4 cut(s) 84, 255, 572, 615
BfoI RGCGCY 1 cut(s) 370
BglI GCCNNNNNGGC 1 cut(s) 416
BisI GCNGC 9 cut(s) 256, 408, 411, 461, 496, 499, 502, 511, 525
BlsI GCNGC 9 cut(s) 257, 409, 412, 462, 497, 500, 503, 512, 526
Bme1390I CCNGG 6 cut(s) 415, 416, 562, 563, 610, 692
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 2 cut(s) 414, 561
BmgBI CACGTC 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 449, 543
BmiI GGNNCC 3 cut(s) 388, 450, 559
BmrFI CCNGG 6 cut(s) 415, 416, 562, 563, 610, 692
BmsI GCATC 1 cut(s) 672
BpmI CTGGAG 1 cut(s) 355
BpuEI CTTGAG 1 cut(s) 463
BpuMI CCSGG 6 cut(s) 415, 416, 562, 563, 610, 692
BsaI GGTCTC 1 cut(s) 572
BsaJI CCNNGG 5 cut(s) 414, 549, 561, 598, 609
BsaXI ACNNNNNCTCC 6 cut(s) 14, 44, 133, 163, 353, 383
Bsc4I CCNNNNNNNGG 4 cut(s) 308, 378, 414, 604
Bse21I CCTNAGG 1 cut(s) 17
BseDI CCNNGG 5 cut(s) 414, 549, 561, 598, 609
BseGI GGATG 1 cut(s) 324
BseLI CCNNNNNNNGG 4 cut(s) 308, 378, 414, 604
BseMII CTCAG 3 cut(s) 187, 216, 621
BseRI GAGGAG 2 cut(s) 40, 138
BseSI GKGCMC 1 cut(s) 562
BseX3I CGGCCG 1 cut(s) 606
BseXI GCAGC 2 cut(s) 267, 488
BsgI GTGCAG 1 cut(s) 274
Bsh1236I CGCG 3 cut(s) 460, 572, 600
Bsh1285I CGRYCG 2 cut(s) 489, 609
BshFI GGCC 5 cut(s) 126, 344, 527, 545, 608
BshNI GGYRCC 1 cut(s) 557
BsiEI CGRYCG 2 cut(s) 489, 609
BsiHKCI CYCGRG 2 cut(s) 414, 561
BsiSI CCGG 5 cut(s) 415, 562, 609, 651, 692
BsiWI CGTACG 1 cut(s) 420
BslI CCNNNNNNNGG 4 cut(s) 308, 378, 414, 604
BsmAI GTCTC 4 cut(s) 84, 255, 572, 615
BsmBI CGTCTC 2 cut(s) 84, 615
BsnI GGCC 5 cut(s) 126, 344, 527, 545, 608
Bso31I GGTCTC 1 cut(s) 572
BsoBI CYCGRG 2 cut(s) 414, 561
Bsp1286I GDGCHC 2 cut(s) 188, 562
Bsp143I GATC 1 cut(s) 661
BspANI GGCC 5 cut(s) 126, 344, 527, 545, 608
BspCNI CTCAG 3 cut(s) 186, 215, 622
BspFNI CGCG 3 cut(s) 460, 572, 600
BspLI GGNNCC 3 cut(s) 388, 450, 559
BspPI GGATC 1 cut(s) 669
BspT107I GGYRCC 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 572
BsrBI CCGCTC 1 cut(s) 284
BssECI CCNNGG 5 cut(s) 414, 549, 561, 598, 609
BssMI GATC 1 cut(s) 661
Bst6I CTCTTC 2 cut(s) 230, 272
BstC8I GCNNGC 1 cut(s) 136
BstDEI CTNAG 5 cut(s) 17, 119, 173, 202, 630
BstDSI CCRYGG 1 cut(s) 598
BstF5I GGATG 1 cut(s) 324
BstFNI CGCG 3 cut(s) 460, 572, 600
BstH2I RGCGCY 1 cut(s) 370
BstHHI GCGC 2 cut(s) 369, 460
BstKTI GATC 1 cut(s) 664
BstMAI GTCTC 4 cut(s) 84, 255, 572, 615
BstMBI GATC 1 cut(s) 661
BstMCI CGRYCG 2 cut(s) 489, 609
BstMWI GCNNNNNNNGC 4 cut(s) 416, 501, 507, 510
BstNSI RCATGY 2 cut(s) 138, 520
BstSCI CCNGG 6 cut(s) 413, 414, 560, 561, 608, 690
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 3 cut(s) 460, 572, 600
BstV1I GCAGC 2 cut(s) 267, 488
BstZI CGGCCG 1 cut(s) 606
Bsu36I CCTNAGG 1 cut(s) 17
BsuRI GGCC 5 cut(s) 126, 344, 527, 545, 608
BtgI CCRYGG 1 cut(s) 598
BtrI CACGTC 1 cut(s) 94
BtsCI GGATG 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 136
CfoI GCGC 2 cut(s) 369, 460
Cfr13I GGNCC 2 cut(s) 449, 543
Cfr42I CCGCGG 1 cut(s) 601
Cfr9I CCCGGG 2 cut(s) 414, 561
CseI GACGC 2 cut(s) 578, 634
Csp6I GTAC 2 cut(s) 304, 421
CspCI CAANNNNNGTGG 2 cut(s) 103, 138
CviAII CATG 2 cut(s) 135, 517
CviQI GTAC 2 cut(s) 304, 421
DdeI CTNAG 5 cut(s) 17, 119, 173, 202, 630
DpnI GATC 1 cut(s) 663
DpnII GATC 1 cut(s) 661
EaeI YGGCCR 3 cut(s) 124, 342, 606
EagI CGGCCG 1 cut(s) 606
Eam1104I CTCTTC 2 cut(s) 230, 272
EarI CTCTTC 2 cut(s) 230, 272
EclXI CGGCCG 1 cut(s) 606
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 572
Eco47I GGWCC 1 cut(s) 449
Eco47III AGCGCT 1 cut(s) 368
Eco52I CGGCCG 1 cut(s) 606
Eco81I CCTNAGG 1 cut(s) 17
Eco88I CYCGRG 2 cut(s) 414, 561
EcoT38I GRGCYC 1 cut(s) 188
Esp3I CGTCTC 2 cut(s) 84, 615
FaeI CATG 2 cut(s) 138, 520
FaiI YATR 3 cut(s) 136, 518, 587
FatI CATG 2 cut(s) 134, 516
FauI CCCGC 1 cut(s) 497
FblI GTMKAC 2 cut(s) 567, 669
Fnu4HI GCNGC 9 cut(s) 256, 408, 411, 461, 496, 499, 502, 511, 525
FokI GGATG 1 cut(s) 331
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 9 cut(s) 256, 408, 411, 461, 496, 499, 502, 511, 525
GlaI GCGC 2 cut(s) 368, 459
GluI GCNGC 9 cut(s) 256, 408, 411, 461, 496, 499, 502, 511, 525
GsuI CTGGAG 1 cut(s) 355
HaeII RGCGCY 1 cut(s) 370
HaeIII GGCC 5 cut(s) 126, 344, 527, 545, 608
HapII CCGG 5 cut(s) 415, 562, 609, 651, 692
HgaI GACGC 2 cut(s) 578, 634
HhaI GCGC 2 cut(s) 369, 460
Hin1II CATG 2 cut(s) 138, 520
Hin6I GCGC 2 cut(s) 367, 458
HinP1I GCGC 2 cut(s) 367, 458
HincII GTYRAC 2 cut(s) 568, 670
HindII GTYRAC 2 cut(s) 568, 670
HinfI GANTC 3 cut(s) 48, 349, 654
HpaII CCGG 5 cut(s) 415, 562, 609, 651, 692
HphI GGTGA 1 cut(s) 719
Hpy166II GTNNAC 2 cut(s) 568, 670
Hpy188I TCNGA 4 cut(s) 89, 176, 205, 703
Hpy188III TCNNGA 3 cut(s) 353, 372, 473
Hpy8I GTNNAC 2 cut(s) 568, 670
Hpy99I CGWCG 3 cut(s) 407, 572, 650
HpyAV CCTTC 3 cut(s) 215, 485, 698
HpyCH4IV ACGT 1 cut(s) 93
HpyCH4V TGCA 3 cut(s) 138, 197, 255
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 501, 507, 510
HpyF3I CTNAG 5 cut(s) 17, 119, 173, 202, 630
HpySE526I ACGT 1 cut(s) 93
Hsp92II CATG 2 cut(s) 138, 520
HspAI GCGC 2 cut(s) 367, 458
KspI CCGCGG 1 cut(s) 601
Kzo9I GATC 1 cut(s) 661
LmnI GCTCC 2 cut(s) 374, 392
LpnPI CCDG 9 cut(s) 120, 259, 385, 428, 486, 575, 622, 664, 705
Lsp1109I GCAGC 2 cut(s) 267, 488
LweI GCATC 1 cut(s) 672
MaeII ACGT 1 cut(s) 93
MaeIII GTNAC 1 cut(s) 33
MalI GATC 1 cut(s) 663
MbiI CCGCTC 1 cut(s) 284
MboI GATC 1 cut(s) 661
MboII GAAGA 5 cut(s) 239, 247, 253, 256, 289
MfeI CAATTG 1 cut(s) 216
MhlI GDGCHC 2 cut(s) 188, 562
MlsI TGGCCA 1 cut(s) 126
MluCI AATT 5 cut(s) 5, 216, 230, 375, 466
MluNI TGGCCA 1 cut(s) 126
Mox20I TGGCCA 1 cut(s) 126
MscI TGGCCA 1 cut(s) 126
MseI TTAA 2 cut(s) 41, 636
Msp20I TGGCCA 1 cut(s) 126
MspA1I CMGCKG 2 cut(s) 258, 600
MspI CCGG 5 cut(s) 415, 562, 609, 651, 692
MspR9I CCNGG 6 cut(s) 415, 416, 562, 563, 610, 692
MunI CAATTG 1 cut(s) 216
MvnI CGCG 3 cut(s) 460, 572, 600
MwoI GCNNNNNNNGC 4 cut(s) 416, 501, 507, 510
NciI CCSGG 6 cut(s) 415, 416, 562, 563, 610, 692
NdeII GATC 1 cut(s) 661
NlaIII CATG 2 cut(s) 138, 520
NlaIV GGNNCC 3 cut(s) 388, 450, 559
NmeAIII GCCGAG 2 cut(s) 363, 370
NmuCI GTSAC 1 cut(s) 33
NspI RCATGY 2 cut(s) 138, 520
PaeI GCATGC 1 cut(s) 138
PciI ACATGT 1 cut(s) 516
PcsI WCGNNNNNNNCGW 1 cut(s) 654
PfeI GAWTC 3 cut(s) 48, 349, 654
Pfl23II CGTACG 1 cut(s) 420
PflMI CCANNNNNTGG 1 cut(s) 378
PkrI GCNGC 9 cut(s) 257, 409, 412, 462, 497, 500, 503, 512, 526
PscI ACATGT 1 cut(s) 516
PspLI CGTACG 1 cut(s) 420
PspN4I GGNNCC 3 cut(s) 388, 450, 559
PspPI GGNCC 2 cut(s) 449, 543
RsaI GTAC 2 cut(s) 305, 422
RsaNI GTAC 2 cut(s) 304, 421
SacII CCGCGG 1 cut(s) 601
SalI GTCGAC 2 cut(s) 566, 668
SaqAI TTAA 2 cut(s) 41, 636
SatI GCNGC 9 cut(s) 256, 408, 411, 461, 496, 499, 502, 511, 525
Sau3AI GATC 1 cut(s) 661
Sau96I GGNCC 2 cut(s) 449, 543
ScrFI CCNGG 6 cut(s) 415, 416, 562, 563, 610, 692
SduI GDGCHC 2 cut(s) 188, 562
SetI ASST 8 cut(s) 32, 58, 65, 96, 120, 158, 555, 709
SfaNI GCATC 1 cut(s) 672
Sfr303I CCGCGG 1 cut(s) 601
SgrBI CCGCGG 1 cut(s) 601
SinI GGWCC 1 cut(s) 449
SmaI CCCGGG 2 cut(s) 416, 563
SmlI CTYRAG 1 cut(s) 442
SmoI CTYRAG 1 cut(s) 442
SphI GCATGC 1 cut(s) 138
SrfI GCCCGGGC 1 cut(s) 416
Sse9I AATT 5 cut(s) 5, 216, 230, 375, 466
StyD4I CCNGG 6 cut(s) 413, 414, 560, 561, 608, 690
TaiI ACGT 1 cut(s) 96
TaqI TCGA 5 cut(s) 51, 352, 489, 567, 669
TaqII GACCGA 1 cut(s) 222
TasI AATT 5 cut(s) 5, 216, 230, 375, 466
TauI GCSGC 7 cut(s) 410, 413, 463, 498, 501, 513, 527
TfiI GAWTC 3 cut(s) 48, 349, 654
Tru1I TTAA 2 cut(s) 41, 636
Tru9I TTAA 2 cut(s) 41, 636
TseFI GTSAC 1 cut(s) 33
TseI GCWGC 2 cut(s) 255, 501
Tsp45I GTSAC 1 cut(s) 33
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 633
TspMI CCCGGG 2 cut(s) 414, 561
Van91I CCANNNNNTGG 1 cut(s) 378
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 1 cut(s) 375
XceI RCATGY 2 cut(s) 138, 520
XmaI CCCGGG 2 cut(s) 414, 561
XmiI GTMKAC 2 cut(s) 567, 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.