Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0007534

Physical Location & Seq

Genomic Coordinates Reverse (-)

28595053 .. 28597816

Gene Structure 2764 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1238000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 639 bp

ATGGGTCTGGCCACAACTAAGGTGATCTTTGTTGTTACTTCTCAACTGCTGCTTGTGGGATTCATGTTCCAGAACATGTGCAAAACTGTGTTCGAGTCTATCATCTTCGTTTTTGTGATGCATCCATTTGATGTTGGTGATCGTTGTGTGGTGGATGGAGTTCAGATGGTTGTAGAAGAGATGAACATTTTGTCAACCGTTTTCTTAAGATACGACAATGAAAAAATATTCTACCCCAATTCTGTGCTTCTTACAAAGCCTATTAGCAATTTCAGAAGGAGTCCAGATATGGCTGACAGTATTGATTTCACAATTGATGTCTCTACTCCAGTTGATGATGTTTCTGCACTCAAGAAGGCCATACAATTATATATTGACAGCAAGCCAAAGTATTGGTCCTCAAAACACTCGGTCATAGTGAAGGAGATTGAGAATGTGGACAAGATGAAAATGATGCTGTGTGTTCAACACACCATGAACCATCAAAATTATGGGGAAAAGAGTGCTCGAAGATCAGAGCTAGTGTTTGAACTGAAGAAGATTTTCCAAAATCTTGGCATAAAGTACCATCTTCTTCCTCAAGAAGTAAACCTCCCGCAATTCAACGCAAGCAATGAGAGGTTAACCATACCTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

213

Amino Acids

24.4

Weight (kDa)

6.96

Isoelectric Point (pI)

40.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	35 - 93	4.9e-11	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007534)

Species	Orthologous Gene IDs
malus_domestica	MD02G1238000.v1.1 MD02G1238200.v1.1 MD07G1078300.v1.1 MD07G1078500.v1.1
prunus_persica	Prupe.2G100800_v2.0.a1
pyrus_communis	pycom02g20350 pycom07g06210 pycom07g06220
rosa_chinensis	RchiOBHm_Chr1g0337341
rosa_laevigata	RLG00000029284
rosa_multiflora	Rmu_sc0006656.1_g000011 Rmu_sc0006824.1_g000012 Rmu_sc0006824.1_g000017 Rmu_sc0018059.1_g000005
rosa_roxburghii	Rroxscaffold_4G00314500
rosa_rugosa	Rorug01G0134500.1
rosa_samantha	Rh1AG153500 Rh1BG122800 Rh1CG144500 Rh1DG161200 Rh6AG271300 Rh6BG515500 Rh6CG521300 Rh6DG507200
rosa_wichuraiana	Rw1G012570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	596
AcoI	YGGCCR	1 cut(s)	9
AcuI	CTGAAG	1 cut(s)	554
AfaI	GTAC	1 cut(s)	566
AflII	CTTAAG	1 cut(s)	205
AflIII	ACRYGT	1 cut(s)	75
AgsI	TTSAA	3 cut(s)	467, 530, 604
AluBI	AGCT	1 cut(s)	520
AluI	AGCT	1 cut(s)	520
Alw21I	GWGCWC	1 cut(s)	508
Alw26I	GTCTC	1 cut(s)	325
AoxI	GGCC	2 cut(s)	9, 357
ApeKI	GCWGC	1 cut(s)	49
ArsI	GACNNNNNNTTYG	2 cut(s)	380, 412
Asp700I	GAANNNNTTC	1 cut(s)	542
AspS9I	GGNCC	1 cut(s)	396
AsuHPI	GGTGA	2 cut(s)	34, 149
AvaII	GGWCC	1 cut(s)	396
BalI	TGGCCA	1 cut(s)	11
Bbv12I	GWGCWC	1 cut(s)	508
BbvI	GCAGC	1 cut(s)	36
BccI	CCATC	4 cut(s)	149, 160, 489, 576
BcoDI	GTCTC	1 cut(s)	325
BfaI	CTAG	1 cut(s)	521
BfrI	CTTAAG	1 cut(s)	205
BisI	GCNGC	1 cut(s)	50
BlsI	GCNGC	1 cut(s)	51
Bme18I	GGWCC	1 cut(s)	396
BmgT120I	GGNCC	1 cut(s)	396
BmsI	GCATC	3 cut(s)	108, 130, 444
BpmI	CTGGAG	1 cut(s)	312
BpuEI	CTTGAG	2 cut(s)	335, 564
Bse1I	ACTGG	1 cut(s)	329
Bse3DI	GCAATG	1 cut(s)	619
BseGI	GGATG	2 cut(s)	121, 160
BseMI	GCAATG	1 cut(s)	619
BseNI	ACTGG	1 cut(s)	329
BseXI	GCAGC	1 cut(s)	36
BsgI	GTGCAG	1 cut(s)	330
BshFI	GGCC	2 cut(s)	11, 359
BsiHKAI	GWGCWC	1 cut(s)	508
BsmAI	GTCTC	1 cut(s)	325
BsnI	GGCC	2 cut(s)	11, 359
Bsp1286I	GDGCHC	1 cut(s)	508
Bsp143I	GATC	3 cut(s)	24, 139, 512
BspACI	CCGC	1 cut(s)	596
BspANI	GGCC	2 cut(s)	11, 359
BspTI	CTTAAG	1 cut(s)	205
BsrDI	GCAATG	1 cut(s)	619
BsrI	ACTGG	1 cut(s)	329
BssMI	GATC	3 cut(s)	24, 139, 512
Bst4CI	ACNGT	3 cut(s)	88, 199, 299
Bst6I	CTCTTC	1 cut(s)	171
BstAFI	CTTAAG	1 cut(s)	205
BstC8I	GCNNGC	2 cut(s)	383, 610
BstDEI	CTNAG	1 cut(s)	18
BstF5I	GGATG	2 cut(s)	121, 160
BstKTI	GATC	3 cut(s)	27, 142, 515
BstMAI	GTCTC	1 cut(s)	325
BstMBI	GATC	3 cut(s)	24, 139, 512
BstNSI	RCATGY	1 cut(s)	79
BstV1I	GCAGC	1 cut(s)	36
BstXI	CCANNNNNNTGG	2 cut(s)	393, 554
BsuRI	GGCC	2 cut(s)	11, 359
BtsCI	GGATG	2 cut(s)	121, 160
Cac8I	GCNNGC	2 cut(s)	383, 610
Cfr13I	GGNCC	1 cut(s)	396
Csp6I	GTAC	1 cut(s)	565
CviAII	CATG	3 cut(s)	64, 76, 475
CviJI	RGCY	6 cut(s)	11, 259, 293, 359, 385, 520
CviKI_1	RGCY	6 cut(s)	11, 259, 293, 359, 385, 520
CviQI	GTAC	1 cut(s)	565
DdeI	CTNAG	1 cut(s)	18
DpnI	GATC	3 cut(s)	26, 141, 514
DpnII	GATC	3 cut(s)	24, 139, 512
EaeI	YGGCCR	1 cut(s)	9
Eam1104I	CTCTTC	1 cut(s)	171
EarI	CTCTTC	1 cut(s)	171
Eco47I	GGWCC	1 cut(s)	396
Eco57I	CTGAAG	1 cut(s)	554
EcoT22I	ATGCAT	1 cut(s)	123
FaeI	CATG	3 cut(s)	67, 79, 478
FalI	AAGNNNNNCTT	2 cut(s)	11, 43
FatI	CATG	3 cut(s)	63, 75, 474
FauI	CCCGC	1 cut(s)	603
Fnu4HI	GCNGC	1 cut(s)	50
FokI	GGATG	2 cut(s)	108, 167
Fsp4HI	GCNGC	1 cut(s)	50
FspBI	CTAG	1 cut(s)	521
GluI	GCNGC	1 cut(s)	50
GsuI	CTGGAG	1 cut(s)	312
HaeIII	GGCC	2 cut(s)	11, 359
Hin1II	CATG	3 cut(s)	67, 79, 478
HincII	GTYRAC	2 cut(s)	195, 624
HindII	GTYRAC	2 cut(s)	195, 624
HinfI	GANTC	3 cut(s)	60, 95, 280
HpaI	GTTAAC	1 cut(s)	624
HphI	GGTGA	2 cut(s)	34, 149
Hpy166II	GTNNAC	4 cut(s)	195, 439, 589, 624
Hpy188I	TCNGA	3 cut(s)	165, 275, 517
Hpy188III	TCNNGA	4 cut(s)	70, 284, 352, 581
Hpy8I	GTNNAC	4 cut(s)	195, 439, 589, 624
HpyAV	CCTTC	3 cut(s)	270, 349, 415
HpyCH4III	ACNGT	3 cut(s)	88, 199, 299
HpyCH4V	TGCA	3 cut(s)	81, 121, 347
HpyF3I	CTNAG	1 cut(s)	18
Hsp92II	CATG	3 cut(s)	67, 79, 478
KspAI	GTTAAC	1 cut(s)	624
Kzo9I	GATC	3 cut(s)	24, 139, 512
LpnPI	CCDG	3 cut(s)	83, 297, 342
Lsp1109I	GCAGC	1 cut(s)	36
LweI	GCATC	3 cut(s)	108, 130, 444
MaeI	CTAG	1 cut(s)	521
MaeIII	GTNAC	1 cut(s)	34
MalI	GATC	3 cut(s)	26, 141, 514
MboI	GATC	3 cut(s)	24, 139, 512
MboII	GAAGA	7 cut(s)	97, 188, 522, 547, 550, 563, 566
MfeI	CAATTG	1 cut(s)	312
MhlI	GDGCHC	1 cut(s)	508
MlsI	TGGCCA	1 cut(s)	11
MluCI	AATT	6 cut(s)	238, 268, 312, 365, 487, 599
MluNI	TGGCCA	1 cut(s)	11
MlyI	GAGTC	2 cut(s)	104, 289
MnlI	CCTC	4 cut(s)	409, 588, 602, 612
Mox20I	TGGCCA	1 cut(s)	11
Mph1103I	ATGCAT	1 cut(s)	123
MroXI	GAANNNNTTC	1 cut(s)	542
MscI	TGGCCA	1 cut(s)	11
MseI	TTAA	3 cut(s)	206, 623, 637
Msp20I	TGGCCA	1 cut(s)	11
MspCI	CTTAAG	1 cut(s)	205
MunI	CAATTG	1 cut(s)	312
NdeII	GATC	3 cut(s)	24, 139, 512
NlaIII	CATG	3 cut(s)	67, 79, 478
NsiI	ATGCAT	1 cut(s)	123
NspI	RCATGY	1 cut(s)	79
PciI	ACATGT	1 cut(s)	75
PdmI	GAANNNNTTC	1 cut(s)	542
PfeI	GAWTC	1 cut(s)	60
PkrI	GCNGC	1 cut(s)	51
PleI	GAGTC	2 cut(s)	103, 288
PpsI	GAGTC	2 cut(s)	103, 288
PscI	ACATGT	1 cut(s)	75
PspPI	GGNCC	1 cut(s)	396
RsaI	GTAC	1 cut(s)	566
RsaNI	GTAC	1 cut(s)	565
SaqAI	TTAA	3 cut(s)	206, 623, 637
SatI	GCNGC	1 cut(s)	50
Sau3AI	GATC	3 cut(s)	24, 139, 512
Sau96I	GGNCC	1 cut(s)	396
SchI	GAGTC	2 cut(s)	104, 289
SduI	GDGCHC	1 cut(s)	508
SetI	ASST	5 cut(s)	24, 522, 594, 623, 634
SfaNI	GCATC	3 cut(s)	108, 130, 444
SinI	GGWCC	1 cut(s)	396
SmlI	CTYRAG	3 cut(s)	205, 350, 579
SmoI	CTYRAG	3 cut(s)	205, 350, 579
Sse9I	AATT	6 cut(s)	238, 268, 312, 365, 487, 599
SsiI	CCGC	1 cut(s)	596
SspI	AATATT	1 cut(s)	228
SspMI	CTAG	1 cut(s)	521
TaaI	ACNGT	3 cut(s)	88, 199, 299
TaqI	TCGA	2 cut(s)	93, 508
TaqII	GACCGA	1 cut(s)	400
TasI	AATT	6 cut(s)	238, 268, 312, 365, 487, 599
TfiI	GAWTC	1 cut(s)	60
Tru1I	TTAA	3 cut(s)	206, 623, 637
Tru9I	TTAA	3 cut(s)	206, 623, 637
TseI	GCWGC	1 cut(s)	49
TspDTI	ATGAA	5 cut(s)	52, 197, 234, 461, 491
Vha464I	CTTAAG	1 cut(s)	205
VpaK11BI	GGWCC	1 cut(s)	396
XceI	RCATGY	1 cut(s)	79
XcmI	CCANNNNNNNNNTGG	1 cut(s)	488
XmnI	GAANNNNTTC	1 cut(s)	542
XspI	CTAG	1 cut(s)	521
Zsp2I	ATGCAT	1 cut(s)	123

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.