Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0007534

Physical Location & Seq

Genomic Coordinates Forward (+)

27862673 .. 27877108

Gene Structure 14436 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G012570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2100 bp

ATGGAGTTCCAAAAGAGCAATACAGACCATGTGGTAGTAAGCATAGACCAACCAAGCCCCAAACTCAACCAGTCACCCCAAGCAGACTCAATCCCACAATCACCACAGTCAAAAGCCAAAACCCTTAGACGCCTCAACTTTTCCAAACCCCGGTCACGGTTCGAGGAGAATAAGCATCCTGTGACACCAAGAACCATTCTCCAATCCGAAGAACACGAGCCCCTAAACACTAACGAAGACAACTACTCCTCCTCATCAGATGATGAAGAATGGTTCGAAAATGAAGAAGACGACCAAGAAGAAGGGGGAAACAAACGCAAGCAAGGGAACAAAAGGAAGAGAAAGATGAACAAGAGAGCTCTGATAGAGTGGACTTTGTTTCTCATAATAATGACTTGCTTGGTATGTTCCCTGACTGTAGATTCCCTTAAACACGAAATGAAATGGGGTTTGCAGGTATGGAAATGGTGCCTAATGGTCTTGGTTATGTTTTGTGGGCGCTTAGTCTCGGGTTGGGTCGTGGGGTTCATTGTCTTTCTTATAGAGAGAAACTTCATGCTTCGAGAAAAAGTACTCTACTTTGTGTTTGGTCTGCGCAAGAGTTTCCAAAATTGTGCTTGGCTAGCCCTAGTTTTAGTGGCTTGGGTGATCATGTTCCCTCATGTCGACAAACAAAGCAAGGTTCTAAAAAAAGTGTTTCGAGCCCTCATCGCCGTGCTGATCGGCGCCACGATTTGGTTGCTAAAGATCGTTTTTGTGAAGGTGTTGGCCTCTTCATTTCACGTAGCTACATTCTTTGACCGTATGAAAGAGAGTGTATTCCACCACTTCATTTTGGATGCCCTGTCCGGGCCCCCAATGGATCTGGAGGAGAGGGAGCACCTTTCGCAGCGTCGTTTGCAGGCTTCCAAGACATTGCCGGCGAGGTTGAGGAGGGATAAGTCTAGCCAGTTGACCAAGTCGAGGTCTTGTAAGCAAGATGGGTCGAGGAGAATTGACATGGAGAAGCTCAAGAAGTTGAGCATGCCGGGTAGGGCAACGGCGTGGAGTGTGAAGAGGCTGGTGAGCTATGTCAGGTCTTCAGGTTTGTCGACAATGGTTTCAAGTACGATGGATGATTTCGGCAATACGGAATCGGAGATTACGAGTGAATGGGAGGCGAGGAACAGTGCTCAAAGAATATTCAAGAATGTTGCCAAGCCTGGTGCCAAATACATAGAAGAGGAAGATTTACTACGGTTCTTGAAGAATGATGAAATTCACACCATATTCCCTCTCTTTGAAGGAGCAACTGAAACTGGTAGAATAACAAAGTCTTCCTTCAGAAATTGGGTGGTACATGCCTACATTGAGCGCAGAGCATTGGCTCATTCTTTGAATGATACCAAGACCGCTGTCCAACAACTTCACAAGCTGGCGAGTGCAGTTGTGATTGTGATTATAACTGTGGTGAGCCTTTTGGTCATGGGTTTGGCCACAACTAAGGTTATCTTTGTGGTCACTTCTCAGTTGCTGCTCGTAGGCTTCATGTTTCAAAACATGTGCAAAACTATGTTTGAGTCCATCATTTTTGTGTTTGTGATGCACCCGTTCGATGTCGGTGATCGTTGCGTCGTCGATGGCGTACAGATGATTGTGGAAGAGATGAACATATTGACAACAGTTTTCTTAAGGTATGATAATGAGAAAATATATTACCCGAATTCTGTGCTTCTTACAAAGCCTATTAGCAATTTCAGAAGAAGTCCAGACATGGGTGACAGTGTTGATTTCACCATTGATGTCTCTACTTCAGTTGACGATGTTACTGCACTCAAGAAGGCTATACAATCATATATTGAGAGCAAACCAAAGCATTGGAACCCGAAACACTCGGTGATAGTGAAGGACATTGTGAATGTGGACAAGATGAAAATGTTGCTATGTGTTCAACATACGATGAACCATCAAAACTATGGGGAAAAGAGTGTTCGAAGATCAGAGCTGGTGTTTGAATTGAAGAGGATATTCCAAAACCTTGGCATTAAGTACCATCTTCTTCCTCAAGAAGTAAATATTCCACAATTCAACAAAAGCACCGGGCTAATTAATCGCGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006950 GO:0006979 GO:0007275 GO:0007568 GO:0008150 GO:0008219 GO:0008381 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009987 GO:0010150 GO:0012501 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0032501 GO:0032502 GO:0033554 GO:0034599 GO:0034614 GO:0036473 GO:0042221 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0050982 GO:0051179 GO:0051234 GO:0051606 GO:0051716 GO:0055085 GO:0070887 GO:0071944 GO:0090693 GO:0097468 GO:0099402 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

699

Amino Acids

80.62

Weight (kDa)

9.37

Isoelectric Point (pI)

51.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	523 - 581	7.1e-11	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007534)

Species	Orthologous Gene IDs
malus_domestica	MD02G1238000.v1.1 MD02G1238200.v1.1 MD07G1078300.v1.1 MD07G1078500.v1.1
prunus_persica	Prupe.2G100800_v2.0.a1
pyrus_communis	pycom02g20350 pycom07g06210 pycom07g06220
rosa_chinensis	RchiOBHm_Chr1g0337341
rosa_laevigata	RLG00000029284
rosa_multiflora	Rmu_sc0006656.1_g000011 Rmu_sc0006824.1_g000012 Rmu_sc0006824.1_g000017 Rmu_sc0018059.1_g000005
rosa_roxburghii	Rroxscaffold_4G00314500
rosa_rugosa	Rorug01G0134500.1
rosa_samantha	Rh1AG153500 Rh1BG122800 Rh1CG144500 Rh1DG161200 Rh6AG271300 Rh6BG515500 Rh6CG521300 Rh6DG507200
rosa_wichuraiana	Rw1G012570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1442
Acc16I	TGCGCA	1 cut(s)	596
Acc36I	ACCTGC	1 cut(s)	445
AccB1I	GGYRCC	3 cut(s)	468, 725, 1205
AccB7I	CCANNNNNTGG	1 cut(s)	735
AccI	GTMKAC	2 cut(s)	666, 1091
AccII	CGCG	1 cut(s)	2094
AciI	CCGC	1 cut(s)	1392
AclWI	GGATC	1 cut(s)	870
AcoI	YGGCCR	1 cut(s)	1473
AcsI	RAATTY	2 cut(s)	1257, 1702
AcuI	CTGAAG	3 cut(s)	1065, 1306, 1776
AcyI	GRCGYC	2 cut(s)	130, 726
AdeI	CACNNNGTG	1 cut(s)	1876
AfaI	GTAC	5 cut(s)	573, 1108, 1338, 1626, 2030
AfiI	CCNNNNNNNGG	6 cut(s)	150, 156, 735, 849, 963, 1754
AflII	CTTAAG	1 cut(s)	1669
AflIII	ACRYGT	1 cut(s)	1539
AjnI	CCWGG	1 cut(s)	1201
AluBI	AGCT	6 cut(s)	359, 788, 1009, 1068, 1414, 1984
AluI	AGCT	6 cut(s)	359, 788, 1009, 1068, 1414, 1984
Alw21I	GWGCWC	3 cut(s)	361, 882, 1174
Alw26I	GTCTC	2 cut(s)	511, 1789
AlwI	GGATC	1 cut(s)	870
AlwNI	CAGNNNCTG	1 cut(s)	1513
Ama87I	CYCGRG	1 cut(s)	508
AoxI	GGCC	3 cut(s)	768, 851, 1473
ApaI	GGGCCC	1 cut(s)	855
ApeKI	GCWGC	2 cut(s)	889, 1513
ApoI	RAATTY	2 cut(s)	1257, 1702
AseI	ATTAAT	1 cut(s)	2088
AspLEI	GCGC	4 cut(s)	501, 597, 728, 1356
AspS9I	GGNCC	2 cut(s)	851, 852
AsuC2I	CCSGG	4 cut(s)	151, 850, 1029, 2080
AsuHPI	GGTGA	9 cut(s)	66, 93, 658, 1075, 1462, 1613, 1765, 1769, 1888
AsuII	TTCGAA	2 cut(s)	276, 1972
AsuNHI	GCTAGC	1 cut(s)	622
AvaI	CYCGRG	1 cut(s)	508
BaeGI	GKGCMC	1 cut(s)	855
BalI	TGGCCA	1 cut(s)	1475
BanI	GGYRCC	3 cut(s)	468, 725, 1205
BanII	GRGCYC	4 cut(s)	222, 361, 706, 855
BarI	GAAGNNNNNNTAC	2 cut(s)	1218, 1250
BauI	CACGAG	1 cut(s)	215
BbsI	GAAGAC	4 cut(s)	243, 294, 1071, 1308
Bbv12I	GWGCWC	3 cut(s)	361, 882, 1174
BbvI	GCAGC	2 cut(s)	901, 1500
BccI	CCATC	6 cut(s)	974, 1105, 1571, 1613, 1953, 2040
BceAI	ACGGC	2 cut(s)	698, 1056
BcgI	CGANNNNNNTGC	6 cut(s)	721, 755, 1690, 1724, 1790, 1824
BciT130I	CCWGG	1 cut(s)	1203
BclI	TGATCA	1 cut(s)	648
BcnI	CCSGG	4 cut(s)	151, 850, 1029, 2080
BcoDI	GTCTC	2 cut(s)	511, 1789
BfaI	CTAG	3 cut(s)	623, 629, 945
BfmI	CTRYAG	1 cut(s)	417
BfoI	RGCGCY	2 cut(s)	502, 729
BfrI	CTTAAG	1 cut(s)	1669
BfuAI	ACCTGC	1 cut(s)	445
BisI	GCNGC	2 cut(s)	890, 1514
BlsI	GCNGC	2 cut(s)	891, 1515
BmcAI	AGTACT	1 cut(s)	573
Bme1390I	CCNGG	5 cut(s)	151, 850, 1029, 1203, 2080
BmeT110I	CYCGRG	1 cut(s)	508
BmgT120I	GGNCC	2 cut(s)	851, 852
BmiI	GGNNCC	6 cut(s)	470, 727, 853, 854, 1207, 1862
BmrFI	CCNGG	5 cut(s)	151, 850, 1029, 1203, 2080
BmsI	GCATC	3 cut(s)	184, 829, 1572
BmtI	GCTAGC	1 cut(s)	626
BoxI	GACNNNNGTC	1 cut(s)	1394
BpiI	GAAGAC	4 cut(s)	243, 294, 1071, 1308
BpmI	CTGGAG	1 cut(s)	887
Bpu14I	TTCGAA	2 cut(s)	276, 1972
BpuEI	CTTGAG	3 cut(s)	995, 1799, 2028
BpuMI	CCSGG	4 cut(s)	151, 850, 1029, 2080
BsaAI	YACGTR	1 cut(s)	784
BsaHI	GRCGYC	2 cut(s)	130, 726
BsaJI	CCNNGG	2 cut(s)	149, 2017
BsaXI	ACNNNNNCTCC	4 cut(s)	230, 233, 260, 263
Bsc4I	CCNNNNNNNGG	6 cut(s)	150, 156, 735, 849, 963, 1754
Bse118I	RCCGGY	1 cut(s)	919
Bse1I	ACTGG	3 cut(s)	70, 949, 1303
Bse3DI	GCAATG	1 cut(s)	914
BseBI	CCWGG	1 cut(s)	1203
BseDI	CCNNGG	2 cut(s)	149, 2017
BseGI	GGATG	3 cut(s)	175, 844, 1120
BseLI	CCNNNNNNNGG	6 cut(s)	150, 156, 735, 849, 963, 1754
BseMI	GCAATG	1 cut(s)	914
BseMII	CTCAG	1 cut(s)	1520
BseNI	ACTGG	3 cut(s)	70, 949, 1303
BseRI	GAGGAG	6 cut(s)	179, 238, 241, 884, 946, 1003
BseSI	GKGCMC	1 cut(s)	855
BseXI	GCAGC	2 cut(s)	901, 1500
BsgI	GTGCAG	2 cut(s)	1443, 1794
Bsh1236I	CGCG	1 cut(s)	2094
BshFI	GGCC	3 cut(s)	770, 853, 1475
BshNI	GGYRCC	3 cut(s)	468, 725, 1205
BsiHKAI	GWGCWC	3 cut(s)	361, 882, 1174
BsiHKCI	CYCGRG	1 cut(s)	508
BsiSI	CCGG	5 cut(s)	151, 849, 920, 1028, 2079
BslI	CCNNNNNNNGG	6 cut(s)	150, 156, 735, 849, 963, 1754
BsmAI	GTCTC	2 cut(s)	511, 1789
BsnI	GGCC	3 cut(s)	770, 853, 1475
BsoBI	CYCGRG	1 cut(s)	508
Bsp119I	TTCGAA	2 cut(s)	276, 1972
Bsp120I	GGGCCC	1 cut(s)	851
Bsp1286I	GDGCHC	6 cut(s)	222, 361, 706, 855, 882, 1174
Bsp143I	GATC	6 cut(s)	648, 720, 747, 862, 1603, 1976
Bsp68I	TCGCGA	1 cut(s)	2094
BspACI	CCGC	1 cut(s)	1392
BspANI	GGCC	3 cut(s)	770, 853, 1475
BspCNI	CTCAG	1 cut(s)	1519
BspFNI	CGCG	1 cut(s)	2094
BspLI	GGNNCC	6 cut(s)	470, 727, 853, 854, 1207, 1862
BspMI	ACCTGC	1 cut(s)	445
BspOI	GCTAGC	1 cut(s)	626
BspPI	GGATC	1 cut(s)	870
BspT104I	TTCGAA	2 cut(s)	276, 1972
BspT107I	GGYRCC	3 cut(s)	468, 725, 1205
BspTI	CTTAAG	1 cut(s)	1669
BsrDI	GCAATG	1 cut(s)	914
BsrFI	RCCGGY	1 cut(s)	919
BsrI	ACTGG	3 cut(s)	70, 949, 1303
BssAI	RCCGGY	1 cut(s)	919
BssECI	CCNNGG	2 cut(s)	149, 2017
BssMI	GATC	6 cut(s)	648, 720, 747, 862, 1603, 1976
BssNI	GRCGYC	2 cut(s)	130, 726
BssSI	CACGAG	1 cut(s)	215
BssT1I	CCWWGG	1 cut(s)	2017
Bst2BI	CACGAG	1 cut(s)	215
Bst2UI	CCWGG	1 cut(s)	1203
Bst4CI	ACNGT	9 cut(s)	108, 159, 418, 803, 1169, 1239, 1447, 1663, 1763
Bst6I	CTCTTC	6 cut(s)	332, 778, 1049, 1215, 1635, 1994
BstACI	GRCGYC	2 cut(s)	130, 726
BstAFI	CTTAAG	1 cut(s)	1669
BstBAI	YACGTR	1 cut(s)	784
BstBI	TTCGAA	2 cut(s)	276, 1972
BstC8I	GCNNGC	6 cut(s)	320, 624, 903, 921, 1025, 1416
BstDEI	CTNAG	4 cut(s)	125, 502, 1482, 1506
BstF5I	GGATG	3 cut(s)	175, 844, 1120
BstFNI	CGCG	1 cut(s)	2094
BstH2I	RGCGCY	2 cut(s)	502, 729
BstHHI	GCGC	4 cut(s)	501, 597, 728, 1356
BstKTI	GATC	6 cut(s)	651, 723, 750, 865, 1606, 1979
BstMAI	GTCTC	2 cut(s)	511, 1789
BstMBI	GATC	6 cut(s)	648, 720, 747, 862, 1603, 1976
BstMWI	GCNNNNNNNGC	4 cut(s)	623, 710, 886, 898
BstNI	CCWGG	1 cut(s)	1203
BstNSI	RCATGY	3 cut(s)	1027, 1343, 1543
BstPAI	GACNNNNGTC	1 cut(s)	1394
BstSCI	CCNGG	5 cut(s)	149, 848, 1027, 1201, 2078
BstSFI	CTRYAG	1 cut(s)	417
BstSLI	GKGCMC	1 cut(s)	855
BstUI	CGCG	1 cut(s)	2094
BstV1I	GCAGC	2 cut(s)	901, 1500
BstV2I	GAAGAC	4 cut(s)	243, 294, 1071, 1308
BstX2I	RGATCY	1 cut(s)	862
BstXI	CCANNNNNNTGG	2 cut(s)	1857, 2018
BstYI	RGATCY	1 cut(s)	862
BsuRI	GGCC	3 cut(s)	770, 853, 1475
BtgZI	GCGATG	1 cut(s)	694
BtsCI	GGATG	3 cut(s)	175, 844, 1120
BtsIMutI	CAGTG	2 cut(s)	1174, 1768
BtuMI	TCGCGA	1 cut(s)	2094
BveI	ACCTGC	1 cut(s)	445
Cac8I	GCNNGC	6 cut(s)	320, 624, 903, 921, 1025, 1416
CaiI	CAGNNNCTG	1 cut(s)	1513
CfoI	GCGC	4 cut(s)	501, 597, 728, 1356
Cfr10I	RCCGGY	1 cut(s)	919
Cfr13I	GGNCC	2 cut(s)	851, 852
CseI	GACGC	3 cut(s)	138, 881, 1600
Csp6I	GTAC	5 cut(s)	572, 1107, 1337, 1625, 2029
CviQI	GTAC	5 cut(s)	572, 1107, 1337, 1625, 2029
DdeI	CTNAG	4 cut(s)	125, 502, 1482, 1506
DinI	GGCGCC	1 cut(s)	727
DpnI	GATC	6 cut(s)	650, 722, 749, 864, 1605, 1978
DpnII	GATC	6 cut(s)	648, 720, 747, 862, 1603, 1976
DraIII	CACNNNGTG	1 cut(s)	1876
EaeI	YGGCCR	1 cut(s)	1473
Eam1104I	CTCTTC	6 cut(s)	332, 778, 1049, 1215, 1635, 1994
EarI	CTCTTC	6 cut(s)	332, 778, 1049, 1215, 1635, 1994
Ecl136II	GAGCTC	1 cut(s)	359
Eco130I	CCWWGG	1 cut(s)	2017
Eco24I	GRGCYC	4 cut(s)	222, 361, 706, 855
Eco53kI	GAGCTC	1 cut(s)	359
Eco57I	CTGAAG	3 cut(s)	1065, 1306, 1776
Eco88I	CYCGRG	1 cut(s)	508
EcoICRI	GAGCTC	1 cut(s)	359
EcoO109I	RGGNCCY	1 cut(s)	852
EcoRI	GAATTC	1 cut(s)	1702
EcoRII	CCWGG	1 cut(s)	1201
EcoT14I	CCWWGG	1 cut(s)	2017
EcoT38I	GRGCYC	4 cut(s)	222, 361, 706, 855
EgeI	GGCGCC	1 cut(s)	727
EheI	GGCGCC	1 cut(s)	727
ErhI	CCWWGG	1 cut(s)	2017
FalI	AAGNNNNNCTT	4 cut(s)	1304, 1336, 1475, 1507
FbaI	TGATCA	1 cut(s)	648
FblI	GTMKAC	2 cut(s)	666, 1091
Fnu4HI	GCNGC	2 cut(s)	890, 1514
FokI	GGATG	3 cut(s)	162, 851, 1127
FriOI	GRGCYC	4 cut(s)	222, 361, 706, 855
Fsp4HI	GCNGC	2 cut(s)	890, 1514
FspBI	CTAG	3 cut(s)	623, 629, 945
FspI	TGCGCA	1 cut(s)	596
GlaI	GCGC	4 cut(s)	500, 596, 727, 1355
GluI	GCNGC	2 cut(s)	890, 1514
GsuI	CTGGAG	1 cut(s)	887
HaeII	RGCGCY	2 cut(s)	502, 729
HaeIII	GGCC	3 cut(s)	770, 853, 1475
HapII	CCGG	5 cut(s)	151, 849, 920, 1028, 2079
HgaI	GACGC	3 cut(s)	138, 881, 1600
HhaI	GCGC	4 cut(s)	501, 597, 728, 1356
Hin1I	GRCGYC	2 cut(s)	130, 726
Hin6I	GCGC	4 cut(s)	499, 595, 726, 1354
HinP1I	GCGC	4 cut(s)	499, 595, 726, 1354
HincII	GTYRAC	4 cut(s)	667, 954, 1092, 1798
HindII	GTYRAC	4 cut(s)	667, 954, 1092, 1798
HinfI	GANTC	4 cut(s)	86, 422, 1133, 1559
HpaII	CCGG	5 cut(s)	151, 849, 920, 1028, 2079
HphI	GGTGA	9 cut(s)	66, 93, 658, 1075, 1462, 1613, 1765, 1769, 1888
Hpy166II	GTNNAC	6 cut(s)	372, 667, 954, 1092, 1798, 1903
Hpy188I	TCNGA	7 cut(s)	208, 259, 363, 1138, 1325, 1739, 1981
Hpy188III	TCNNGA	9 cut(s)	563, 866, 1012, 1186, 1243, 1748, 1816, 2045, 2093
Hpy8I	GTNNAC	6 cut(s)	372, 667, 954, 1092, 1798, 1903
Hpy99I	CGWCG	3 cut(s)	897, 1616, 1619
HpyAV	CCTTC	6 cut(s)	296, 754, 1277, 1330, 1813, 1879
HpyCH4III	ACNGT	9 cut(s)	108, 159, 418, 803, 1169, 1239, 1447, 1663, 1763
HpyCH4IV	ACGT	1 cut(s)	783
HpyCH4V	TGCA	6 cut(s)	454, 901, 1424, 1545, 1585, 1811
HpyF10VI	GCNNNNNNNGC	4 cut(s)	623, 710, 886, 898
HpyF3I	CTNAG	4 cut(s)	125, 502, 1482, 1506
HpySE526I	ACGT	1 cut(s)	783
Hsp92I	GRCGYC	2 cut(s)	130, 726
HspAI	GCGC	4 cut(s)	499, 595, 726, 1354
KasI	GGCGCC	1 cut(s)	725
KroI	GCCGGC	1 cut(s)	919
KroNI	GCCGGC	1 cut(s)	921
Ksp22I	TGATCA	1 cut(s)	648
Kzo9I	GATC	6 cut(s)	648, 720, 747, 862, 1603, 1976
LmnI	GCTCC	2 cut(s)	877, 1286
Lsp1109I	GCAGC	2 cut(s)	901, 1500
LweI	GCATC	3 cut(s)	184, 829, 1572
MaeI	CTAG	3 cut(s)	623, 629, 945
MaeII	ACGT	1 cut(s)	783
MaeIII	GTNAC	6 cut(s)	72, 153, 181, 1498, 1757, 1804
MalI	GATC	6 cut(s)	650, 722, 749, 864, 1605, 1978
MboI	GATC	6 cut(s)	648, 720, 747, 862, 1603, 1976
MflI	RGATCY	1 cut(s)	862
MhlI	GDGCHC	6 cut(s)	222, 361, 706, 855, 882, 1174
MlsI	TGGCCA	1 cut(s)	1475
MluCI	AATT	9 cut(s)	610, 993, 1257, 1327, 1702, 1732, 1994, 2063, 2085
MluNI	TGGCCA	1 cut(s)	1475
Mly113I	GGCGCC	1 cut(s)	726
MlyI	GAGTC	2 cut(s)	80, 1568
MmeI	TCCRAC	1 cut(s)	1423
Mox20I	TGGCCA	1 cut(s)	1475
MroNI	GCCGGC	1 cut(s)	919
MscI	TGGCCA	1 cut(s)	1475
MseI	TTAA	5 cut(s)	429, 1670, 2025, 2088, 2098
MslI	CAYNNNNRTG	3 cut(s)	389, 713, 1571
Msp20I	TGGCCA	1 cut(s)	1475
MspA1I	CMGCKG	1 cut(s)	1394
MspCI	CTTAAG	1 cut(s)	1669
MspI	CCGG	5 cut(s)	151, 849, 920, 1028, 2079
MspR9I	CCNGG	5 cut(s)	151, 850, 1029, 1203, 2080
MvaI	CCWGG	1 cut(s)	1203
MvnI	CGCG	1 cut(s)	2094
MwoI	GCNNNNNNNGC	4 cut(s)	623, 710, 886, 898
NaeI	GCCGGC	1 cut(s)	921
NarI	GGCGCC	1 cut(s)	726
NciI	CCSGG	4 cut(s)	151, 850, 1029, 2080
NdeII	GATC	6 cut(s)	648, 720, 747, 862, 1603, 1976
NgoMIV	GCCGGC	1 cut(s)	919
NheI	GCTAGC	1 cut(s)	622
NlaIV	GGNNCC	6 cut(s)	470, 727, 853, 854, 1207, 1862
NmuCI	GTSAC	5 cut(s)	72, 153, 181, 1498, 1757
NruI	TCGCGA	1 cut(s)	2094
NsbI	TGCGCA	1 cut(s)	596
NspI	RCATGY	3 cut(s)	1027, 1343, 1543
NspV	TTCGAA	2 cut(s)	276, 1972
PaeI	GCATGC	1 cut(s)	1027
PciI	ACATGT	1 cut(s)	1539
PcsI	WCGNNNNNNNCGW	2 cut(s)	1142, 1620
PdiI	GCCGGC	1 cut(s)	921
PfeI	GAWTC	2 cut(s)	422, 1133
PflFI	GACNNNGTC	1 cut(s)	958
PflMI	CCANNNNNTGG	1 cut(s)	735
PkrI	GCNGC	2 cut(s)	891, 1515
PleI	GAGTC	2 cut(s)	80, 1567
PluTI	GGCGCC	1 cut(s)	729
PpsI	GAGTC	2 cut(s)	80, 1567
Ppu21I	YACGTR	1 cut(s)	784
PscI	ACATGT	1 cut(s)	1539
PshAI	GACNNNNGTC	1 cut(s)	1394
PshBI	ATTAAT	1 cut(s)	2088
PsiI	TTATAA	1 cut(s)	1442
Psp124BI	GAGCTC	1 cut(s)	361
Psp6I	CCWGG	1 cut(s)	1201
PspGI	CCWGG	1 cut(s)	1201
PspN4I	GGNNCC	6 cut(s)	470, 727, 853, 854, 1207, 1862
PspOMI	GGGCCC	1 cut(s)	851
PspPI	GGNCC	2 cut(s)	851, 852
PstNI	CAGNNNCTG	1 cut(s)	1513
PsuI	RGATCY	1 cut(s)	862
PsyI	GACNNNGTC	1 cut(s)	958
RruI	TCGCGA	1 cut(s)	2094
RsaI	GTAC	5 cut(s)	573, 1108, 1338, 1626, 2030
RsaNI	GTAC	5 cut(s)	572, 1107, 1337, 1625, 2029
RseI	CAYNNNNRTG	3 cut(s)	389, 713, 1571
SacI	GAGCTC	1 cut(s)	361
SalI	GTCGAC	2 cut(s)	665, 1090
SaqAI	TTAA	5 cut(s)	429, 1670, 2025, 2088, 2098
SatI	GCNGC	2 cut(s)	890, 1514
Sau3AI	GATC	6 cut(s)	648, 720, 747, 862, 1603, 1976
Sau96I	GGNCC	2 cut(s)	851, 852
ScaI	AGTACT	1 cut(s)	573
SchI	GAGTC	2 cut(s)	80, 1568
ScrFI	CCNGG	5 cut(s)	151, 850, 1029, 1203, 2080
SduI	GDGCHC	6 cut(s)	222, 361, 706, 855, 882, 1174
SfaNI	GCATC	3 cut(s)	184, 829, 1572
SfcI	CTRYAG	1 cut(s)	417
SfoI	GGCGCC	1 cut(s)	727
SfuI	TTCGAA	2 cut(s)	276, 1972
SmiMI	CAYNNNNRTG	3 cut(s)	389, 713, 1571
SmlI	CTYRAG	4 cut(s)	1010, 1669, 1814, 2043
SmoI	CTYRAG	4 cut(s)	1010, 1669, 1814, 2043
SphI	GCATGC	1 cut(s)	1027
Sse9I	AATT	9 cut(s)	610, 993, 1257, 1327, 1702, 1732, 1994, 2063, 2085
SsiI	CCGC	1 cut(s)	1392
SspDI	GGCGCC	1 cut(s)	725
SspI	AATATT	2 cut(s)	1182, 2056
SspMI	CTAG	3 cut(s)	623, 629, 945
SstI	GAGCTC	1 cut(s)	361
StyD4I	CCNGG	5 cut(s)	149, 848, 1027, 1201, 2078
StyI	CCWWGG	1 cut(s)	2017
TaaI	ACNGT	9 cut(s)	108, 159, 418, 803, 1169, 1239, 1447, 1663, 1763
TaiI	ACGT	1 cut(s)	786
TasI	AATT	9 cut(s)	610, 993, 1257, 1327, 1702, 1732, 1994, 2063, 2085
TatI	WGTACW	1 cut(s)	571
TfiI	GAWTC	2 cut(s)	422, 1133
Tru1I	TTAA	5 cut(s)	429, 1670, 2025, 2088, 2098
Tru9I	TTAA	5 cut(s)	429, 1670, 2025, 2088, 2098
TscAI	CASTG	2 cut(s)	1174, 1768
TseFI	GTSAC	5 cut(s)	72, 153, 181, 1498, 1757
TseI	GCWGC	2 cut(s)	889, 1513
Tsp45I	GTSAC	5 cut(s)	72, 153, 181, 1498, 1757
TspGWI	ACGGA	1 cut(s)	1145
TspRI	CASTG	2 cut(s)	1174, 1768
Tth111I	GACNNNGTC	1 cut(s)	958
Van91I	CCANNNNNTGG	1 cut(s)	735
Vha464I	CTTAAG	1 cut(s)	1669
VspI	ATTAAT	1 cut(s)	2088
XapI	RAATTY	2 cut(s)	1257, 1702
XceI	RCATGY	3 cut(s)	1027, 1343, 1543
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1952
XmiI	GTMKAC	2 cut(s)	666, 1091
XspI	CTAG	3 cut(s)	623, 629, 945
ZrmI	AGTACT	1 cut(s)	573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.