Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002501

Physical Location & Seq

Genomic Coordinates Reverse (-)

32476857 .. 32486718

Gene Structure 9862 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1270000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3252 bp

ATGGATCACGGTCGCTATGGTCATCAGCAAAGATGGGATAATAACAGCGCTCTGGAGGGGTATGGTGCAGTCCACGAGCCAAACTTTCGGGCCAATGGTTCATATGATGAGAGGAGGTTTATAGATGAAAGATGCTCAAGGGACAACGTCTATCCAAGAAACTCTTTTCACCGTGATGTTCTAGATGGGGATAACTATCCACCTCCACCTCCTCATGCTGTTGGTGTCTGGCCACACTCAAGAAGGAGAAGTTATGAGGAAGATCTTCCAATTGAGAGGGAAACTAGGCGACATGAGAAGCAATACATGGATTCATATCACGAGATGGATAACTTTCTTGATCATGACAGCGATACGTTTCAGGATTTTGATAAATTTCGGGATAGCTATCGTAGTGTCAACTATCGTGATCCTGGGTTTGACAGGATTGCTCGGTTTGGTGGACGTGATCGTGATGATTATGCTTATGATGATTTTGACTCTAGGCCCCGTTCTACCCATCAGAACAGGGAGGATAGTCGTGAAAGGGATTATGACTATGGTCGATACAGTTATGATTCTGATTACGATGGAAGTAGTAGGAGAGATGGCAATTGGAGGTGTCGGGGATCTCGTGAATCTCGTGACCGAGAGCGTGACAAGAAATGTTTGAGTAGGGAGACAGAACAGAGTCCACATAGAAGGCATGAGCAGTCCCGTTCTCGGTCCATTTCTCGTGGGCATGATGATCGCCCTAGATCAAGGTCCCCTAGAGGTCGAAGCCATGGGCGAAGTCATCGAGAGGACAGCTATGATGATGGACGGTACGAGGGAACTGATAAGCGAAGGGATCGTGATGAGAAACGCCATAGAGAACATTATTCTGTGGCCCCATCTGCAACTATTGTTGTGAAGGGTCTTTCCCAGAAGACAAATGAGGAAGATTTATACCAGATCCTTGCTGAATGGGGACCCCTTCGACATGTCCGTGTGATCAAAGAACGTAATTCTGGCACTTCTCGTGGTTTTGCGTTTATTGATTTTCCTTCTGTGGGAGCAGCACGCGCTATGATGGACAGCATGGGGGATGAAGGGCTTGTGGTCAATGACAGGAAGCTTTTTTTCGAGTATAGTAGTAAGCCAACTGGGGGTGCAGGTGGATCTTTTGGTCAGGAGAATTCCATTAAATTAGGCCATAATACCCATAATACCCATAAAAGCACCACAATGCCATCTGATTGGATGTGCATAAGTTGTGAATATGTTAATTTTGCACGGCGCACTTCCTGTTTTCAGTGCAATAAGGCACGAACTGAGGATGCTCCTGCAGCAGATATTTCTTTGTCCAATCAAATGACTTCAGCAAAGAAGGGGTCTGAAGCTGGTCCTACTCATGTCTTGGTTGTTCGTGGACTGGATGAAAATGCTGATGAGGAAATGCTGCGCTATGAGTTCTCCAAACATGCTGCAATTAAGGATCTTCGTCTTGTTAGAGACAAATTTACTCATGTTTCAAGGGGGTTTGCTTTTGTACATTTCCATTCGGTAGGAGATGCTACAAAAGCTCTTGAAGCAACAAATGGAACAACACTTGAGAGGAATGGGCAAATTTTGAGAGTAGCATATGCAAAGAGCGTTCTTGGTCCAGGATCTTCACAGTCAAGCAGCCTGGCAGCTGCTGCGATTGAGGCAGCCACATTTGCCCAACAGTACGATGCTGTTGGATGGGCTCCAAAGGAATATAATCCAGATGACAAACAATCCACTGCAGGGAACGAGCAAAATGGCGGGGAGATGGCAGTACAACAAGATGGTTTGGCTCCACAATCTGGTTTTGTGTGGGATGAGGCATCTGGTTATTACTATGATGCTGCTTCTGGGTTCTACTATGACCCAAATACAGGCCTTTACTATGATGGTAACAACGGCATTTGGTATACATATGACAACCAAACTCAGCAGTACATCCCTTGCACTGATCAAAATGATAACAAAACATCTGCTGATCAATCTGAGCTTTCCAAGGCATCGGATGGTTCAAGTAATAGAAAAGCAGTCATTTCTGCTCCAGCTACAACCTCAACATCTGAGAAGGCTGCATCATTACCAGATGCAGTCCAGGCTGCTGCAGCAGCAGCAATAGCTGCAGAGAAAAGGGAAAAGGAAAAGGCAAAAGAGATCAAACTGGCTTCAAAGAGCAGTATTTTGGCAAACAAGAAGAAAATGAGTAATGTGTTGACAATGTGGAAGCAAAGGAGTCACGAAGGGCAAGCAACCCGTGTAGCTCTGGATGACAACCAGCCAAGTGGTTCGGCTGATGATAGACCAGTTTCTTCTGTGCAGTCTGCAAAGAGCAAGTTTAAAACTAATGTGACAAAGGAGAATACTACATCCAGTTCAGGGGTTACTGCAACTGTTTCTGCTGCACAGATTACATCCAGTTCAGGGGTTACTGACACTGTTTCTGCTGCAGAGACTGTTGGTTTGGAGTGTTCAGTTATGCCAAGGCCTGTGAGTAACAGTATAAGAGGGACAGTGATGGGAGTCATAAGGGGATCTGGAAGAGGTGTAGTGAAATCAGATGCCTCGTTTTCAGGATCAAGTGTTGGAGTTTCCATGCCTTCAACTTCTGCTCCCGGCATGGCAGGTTCATCTGCATCAACAAATGCAGTCACCCCCACAGTTTTGACACCCTTTAGAACGGATGCATCTGCATTGGGTTCTTATACACCAGCTGTTGCTACTGGGAGTGGAAAGAGAAGATTTTCTGAAATGCCACTAACTCCTGCTTCTGCTCCTAAAGAACCTCATACTGCGTACAGGGATCGTGCAGCCGAGAGAAGAAGCTTATATGGTTCATCGGTGTCTTTTAGTGATGACGTATCTGACCTTGGTTTTGCAGAGTCAAATCAAGATTCTGCGTCAAGAAAGGGTTCTTTTGATTCAATGCCTTTTCCCCCTGGTGTTGGTGGAGGACGTGGGGTTGGAGATGCTAACATTGACAGTTACGAGGTGATTACAGCTGAAAAAGCAATTGATGAGAGCAATGTGGGCAACAAGATGCTTCGCAACATGGGCTGGCACGAAGGCTTGGGGTTAGGAAGAGATGGAAGCGGAATGGTAGAACCAGTGCAGGCTCAGTCCGTGGAACGGAGAGCAGGACTTGGGAGTCAGCAGAAGAAGTTAGATCCTACTCTCGAGGTGCAGGCCGGAGATAGTTACAAGACTCTCATTCAGAAGAAGGCACTGGCCAGGTTCCGGGAGATGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000375 GO:0000377 GO:0000381 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0010468 GO:0010941 GO:0010942 GO:0016043 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0031323 GO:0034622 GO:0034641 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048024 GO:0048518 GO:0048522 GO:0050684 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065003 GO:0065007 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1903311

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-patch RRM_1

Protein Analysis

1084

Amino Acids

119.56

Weight (kDa)

6.68

Isoelectric Point (pI)

52.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	295 - 354	1.9e-12	RNA recognition motif
RRM_1	PF00076	460 - 525	3.9e-16	RNA recognition motif
OCRE	PF17780	601 - 650	7.6e-20	OCRE domain
G-patch_2	PF12656	1005 - 1053	7.2e-07	G-patch domain
G-patch	PF01585	1008 - 1049	1.1e-13	G-patch domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002501)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54230 AT3G54230 AT3G54230 AT3G54230 AT3G54230
fragaria_vesca	FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410
malus_domestica	MD00G1152000.v1.1 MD02G1270000.v1.1
prunus_persica	Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1
pyrus_communis	pycom02g23120 pycom07g03700
rosa_chinensis	RchiOBHm_Chr1g0329501
rosa_laevigata	RLG00000029881
rosa_multiflora	Rmu_sc0004805.1_g000032 Rmu_sc0016170.1_g000004
rosa_roxburghii	Rroxscaffold_4G00321020
rosa_rugosa	Rorug01G0081000 Rorug01G0081100
rosa_samantha	Rh1AG099500 Rh1BG079200 Rh1CG095400 Rh1DG103200
rosa_wichuraiana	Rw0G014300 Rw1G007750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1124
AasI	GACNNNNNNGTC	1 cut(s)	3147
Acc36I	ACCTGC	2 cut(s)	1124, 2615
AccB7I	CCANNNNNTGG	1 cut(s)	1808
AccI	GTMKAC	1 cut(s)	1916
AccII	CGCG	1 cut(s)	1044
AciI	CCGC	2 cut(s)	1767, 3093
AcoI	YGGCCR	2 cut(s)	230, 3228
AcsI	RAATTY	4 cut(s)	374, 1156, 1478, 1587
AcuI	CTGAAG	2 cut(s)	1323, 1377
AfaI	GTAC	6 cut(s)	806, 1512, 1691, 1782, 1943, 2798
AfeI	AGCGCT	1 cut(s)	49
AflIII	ACRYGT	1 cut(s)	961
AgsI	TTSAA	6 cut(s)	1494, 1550, 2019, 2172, 2604, 2925
AjiI	CACGTC	2 cut(s)	446, 2957
AjnI	CCWGG	6 cut(s)	412, 1624, 1647, 2097, 2938, 3230
Alw26I	GTCTC	3 cut(s)	653, 1467, 2447
AlwNI	CAGNNNCTG	2 cut(s)	1658, 2456
Ama87I	CYCGRG	1 cut(s)	3176
Aor51HI	AGCGCT	1 cut(s)	49
AoxI	GGCC	9 cut(s)	90, 230, 485, 867, 1171, 1882, 2486, 3186, 3228
ApoI	RAATTY	4 cut(s)	374, 1156, 1478, 1587
ArsI	GACNNNNNNTTYG	6 cut(s)	1337, 1369, 2446, 2478, 2858, 2890
Asp700I	GAANNNNTTC	3 cut(s)	264, 2743, 2911
AspLEI	GCGC	4 cut(s)	50, 1046, 1260, 1425
AspS9I	GGNCC	8 cut(s)	90, 486, 705, 744, 868, 950, 1364, 1622
AsuC2I	CCSGG	2 cut(s)	2616, 3239
AsuHPI	GGTGA	3 cut(s)	161, 2644, 3004
AvaI	CYCGRG	1 cut(s)	3176
AvaII	GGWCC	5 cut(s)	705, 744, 950, 1364, 1622
BalI	TGGCCA	2 cut(s)	232, 3230
BanII	GRGCYC	1 cut(s)	1711
BauI	CACGAG	6 cut(s)	74, 320, 612, 621, 714, 999
BbsI	GAAGAC	1 cut(s)	914
BceAI	ACGGC	2 cut(s)	1271, 1921
BcgI	CGANNNNNNTGC	2 cut(s)	710, 744
BciT130I	CCWGG	6 cut(s)	414, 1626, 1649, 2099, 2940, 3232
BclI	TGATCA	4 cut(s)	340, 972, 1957, 1984
BcnI	CCSGG	2 cut(s)	2616, 3239
BcoDI	GTCTC	3 cut(s)	653, 1467, 2447
BfaI	CTAG	5 cut(s)	182, 285, 483, 735, 750
BfmI	CTRYAG	5 cut(s)	1305, 1746, 2106, 2124, 2448
BfoI	RGCGCY	1 cut(s)	51
BfuAI	ACCTGC	2 cut(s)	1124, 2615
BglII	AGATCT	1 cut(s)	262
Bme1390I	CCNGG	8 cut(s)	414, 1626, 1649, 2099, 2616, 2940, 3232, 3239
Bme18I	GGWCC	5 cut(s)	705, 744, 950, 1364, 1622
BmeT110I	CYCGRG	1 cut(s)	3176
BmgBI	CACGTC	2 cut(s)	446, 2957
BmgT120I	GGNCC	8 cut(s)	90, 486, 705, 744, 868, 950, 1364, 1622
BmiI	GGNNCC	8 cut(s)	488, 746, 870, 951, 952, 1710, 1800, 3236
BmrFI	CCNGG	8 cut(s)	414, 1626, 1649, 2099, 2616, 2940, 3232, 3239
BmrI	ACTGGG	2 cut(s)	1134, 2733
BmuI	ACTGGG	2 cut(s)	1134, 2733
BoxI	GACNNNNGTC	1 cut(s)	540
BpiI	GAAGAC	1 cut(s)	914
BpmI	CTGGAG	2 cut(s)	74, 2031
BpuEI	CTTGAG	3 cut(s)	121, 223, 1592
BpuMI	CCSGG	2 cut(s)	2616, 3239
BsaBI	GATNNNNATC	3 cut(s)	195, 315, 387
BsaJI	CCNNGG	7 cut(s)	413, 763, 2001, 2483, 2869, 2938, 3123
BsaXI	ACNNNNNCTCC	2 cut(s)	2596, 2626
Bse1I	ACTGG	9 cut(s)	1129, 1398, 2169, 2306, 2373, 2418, 2728, 3107, 3231
Bse3DI	GCAATG	1 cut(s)	3031
Bse8I	GATNNNNATC	3 cut(s)	195, 315, 387
BseBI	CCWGG	6 cut(s)	414, 1626, 1649, 2099, 2940, 3232
BseDI	CCNNGG	7 cut(s)	413, 763, 2001, 2483, 2869, 2938, 3123
BseJI	GATNNNNATC	3 cut(s)	195, 315, 387
BseMI	GCAATG	1 cut(s)	3031
BseMII	CTCAG	5 cut(s)	1284, 1949, 1983, 2058, 3131
BseNI	ACTGG	9 cut(s)	1129, 1398, 2169, 2306, 2373, 2418, 2728, 3107, 3231
BseRI	GAGGAG	2 cut(s)	127, 201
BsgI	GTGCAG	7 cut(s)	87, 1152, 2339, 2388, 2829, 3131, 3203
Bsh1236I	CGCG	1 cut(s)	1044
Bsh1285I	CGRYCG	1 cut(s)	13
BshFI	GGCC	9 cut(s)	92, 232, 487, 869, 1173, 1884, 2488, 3188, 3230
BsiEI	CGRYCG	1 cut(s)	13
BsiHKCI	CYCGRG	1 cut(s)	3176
BsiSI	CCGG	3 cut(s)	2616, 3189, 3238
BslFI	GGGAC	5 cut(s)	155, 679, 730, 963, 2524
BsmAI	GTCTC	3 cut(s)	653, 1467, 2447
BsmFI	GGGAC	5 cut(s)	155, 679, 730, 963, 2524
BsnI	GGCC	9 cut(s)	92, 232, 487, 869, 1173, 1884, 2488, 3188, 3230
BsoBI	CYCGRG	1 cut(s)	3176
Bsp1286I	GDGCHC	1 cut(s)	1711
Bsp1407I	TGTACA	1 cut(s)	1510
Bsp19I	CCATGG	1 cut(s)	763
BspACI	CCGC	2 cut(s)	1767, 3093
BspANI	GGCC	9 cut(s)	92, 232, 487, 869, 1173, 1884, 2488, 3188, 3230
BspCNI	CTCAG	5 cut(s)	1285, 1948, 1984, 2059, 3130
BspFNI	CGCG	1 cut(s)	1044
BspHI	TCATGA	1 cut(s)	343
BspLI	GGNNCC	8 cut(s)	488, 746, 870, 951, 952, 1710, 1800, 3236
BspMAI	CTGCAG	5 cut(s)	1309, 1750, 2110, 2128, 2452
BspMI	ACCTGC	2 cut(s)	1124, 2615
BsrDI	GCAATG	1 cut(s)	3031
BsrGI	TGTACA	1 cut(s)	1510
BsrI	ACTGG	9 cut(s)	1129, 1398, 2169, 2306, 2373, 2418, 2728, 3107, 3231
BssECI	CCNNGG	7 cut(s)	413, 763, 2001, 2483, 2869, 2938, 3123
BssNAI	GTATAC	1 cut(s)	1917
BssSI	CACGAG	6 cut(s)	74, 320, 612, 621, 714, 999
BssT1I	CCWWGG	4 cut(s)	763, 2001, 2483, 2869
Bst1107I	GTATAC	1 cut(s)	1917
Bst2BI	CACGAG	6 cut(s)	74, 320, 612, 621, 714, 999
Bst2UI	CCWGG	6 cut(s)	414, 1626, 1649, 2099, 2940, 3232
Bst6I	CTCTTC	2 cut(s)	2536, 3076
BstAPI	GCANNNNNTGC	2 cut(s)	1658, 2123
BstAUI	TGTACA	1 cut(s)	1510
BstC8I	GCNNGC	5 cut(s)	1042, 2250, 3059, 3114, 3186
BstDEI	CTNAG	5 cut(s)	1293, 1935, 1992, 2067, 3117
BstDSI	CCRYGG	2 cut(s)	763, 3123
BstFNI	CGCG	1 cut(s)	1044
BstH2I	RGCGCY	1 cut(s)	51
BstHHI	GCGC	4 cut(s)	50, 1046, 1260, 1425
BstMAI	GTCTC	3 cut(s)	653, 1467, 2447
BstMCI	CGRYCG	1 cut(s)	13
BstNI	CCWGG	6 cut(s)	414, 1626, 1649, 2099, 2940, 3232
BstNSI	RCATGY	2 cut(s)	965, 1445
BstPAI	GACNNNNGTC	1 cut(s)	540
BstSCI	CCNGG	8 cut(s)	412, 1624, 1647, 2097, 2614, 2938, 3230, 3237
BstSFI	CTRYAG	5 cut(s)	1305, 1746, 2106, 2124, 2448
BstUI	CGCG	1 cut(s)	1044
BstV2I	GAAGAC	1 cut(s)	914
BstX2I	RGATCY	8 cut(s)	262, 608, 933, 1139, 1456, 1628, 2534, 3166
BstXI	CCANNNNNNTGG	2 cut(s)	1218, 2285
BstYI	RGATCY	8 cut(s)	262, 608, 933, 1139, 1456, 1628, 2534, 3166
BstZ17I	GTATAC	1 cut(s)	1917
BsuRI	GGCC	9 cut(s)	92, 232, 487, 869, 1173, 1884, 2488, 3188, 3230
BtgI	CCRYGG	2 cut(s)	763, 3123
BtrI	CACGTC	2 cut(s)	446, 2957
BtsI	GCAGTG	1 cut(s)	1743
BtsIMutI	CAGTG	7 cut(s)	1280, 1743, 1953, 2436, 2520, 3114, 3224
BveI	ACCTGC	2 cut(s)	1124, 2615
Cac8I	GCNNGC	5 cut(s)	1042, 2250, 3059, 3114, 3186
CaiI	CAGNNNCTG	2 cut(s)	1658, 2456
CciI	TCATGA	1 cut(s)	343
CfoI	GCGC	4 cut(s)	50, 1046, 1260, 1425
Cfr13I	GGNCC	8 cut(s)	90, 486, 705, 744, 868, 950, 1364, 1622
CseI	GACGC	1 cut(s)	2889
Csp6I	GTAC	6 cut(s)	805, 1511, 1690, 1781, 1942, 2797
CviQI	GTAC	6 cut(s)	805, 1511, 1690, 1781, 1942, 2797
DdeI	CTNAG	5 cut(s)	1293, 1935, 1992, 2067, 3117
DraI	TTTAAA	1 cut(s)	2341
DrdI	GACNNNNNNGTC	1 cut(s)	3147
DseDI	GACNNNNNNGTC	1 cut(s)	3147
EaeI	YGGCCR	2 cut(s)	230, 3228
Eam1104I	CTCTTC	2 cut(s)	2536, 3076
EarI	CTCTTC	2 cut(s)	2536, 3076
Eco130I	CCWWGG	4 cut(s)	763, 2001, 2483, 2869
Eco147I	AGGCCT	2 cut(s)	1884, 2488
Eco24I	GRGCYC	1 cut(s)	1711
Eco47I	GGWCC	5 cut(s)	705, 744, 950, 1364, 1622
Eco47III	AGCGCT	1 cut(s)	49
Eco57I	CTGAAG	2 cut(s)	1323, 1377
Eco88I	CYCGRG	1 cut(s)	3176
EcoO109I	RGGNCCY	3 cut(s)	486, 744, 950
EcoRI	GAATTC	1 cut(s)	1156
EcoRII	CCWGG	6 cut(s)	412, 1624, 1647, 2097, 2938, 3230
EcoT14I	CCWWGG	4 cut(s)	763, 2001, 2483, 2869
EcoT22I	ATGCAT	1 cut(s)	2689
EcoT38I	GRGCYC	1 cut(s)	1711
ErhI	CCWWGG	4 cut(s)	763, 2001, 2483, 2869
FalI	AAGNNNNNCTT	2 cut(s)	148, 180
FaqI	GGGAC	5 cut(s)	155, 679, 730, 963, 2524
FauI	CCCGC	1 cut(s)	1760
FauNDI	CATATG	3 cut(s)	103, 1603, 1921
FbaI	TGATCA	4 cut(s)	340, 972, 1957, 1984
FblI	GTMKAC	1 cut(s)	1916
FriOI	GRGCYC	1 cut(s)	1711
FspBI	CTAG	5 cut(s)	182, 285, 483, 735, 750
GlaI	GCGC	4 cut(s)	49, 1045, 1259, 1424
GsuI	CTGGAG	2 cut(s)	74, 2031
HaeII	RGCGCY	1 cut(s)	51
HaeIII	GGCC	9 cut(s)	92, 232, 487, 869, 1173, 1884, 2488, 3188, 3230
HapII	CCGG	3 cut(s)	2616, 3189, 3238
HgaI	GACGC	1 cut(s)	2889
HhaI	GCGC	4 cut(s)	50, 1046, 1260, 1425
Hin6I	GCGC	4 cut(s)	48, 1044, 1258, 1423
HinP1I	GCGC	4 cut(s)	48, 1044, 1258, 1423
HincII	GTYRAC	2 cut(s)	400, 2217
HindII	GTYRAC	2 cut(s)	400, 2217
HindIII	AAGCTT	2 cut(s)	1094, 2824
HpaII	CCGG	3 cut(s)	2616, 3189, 3238
HphI	GGTGA	3 cut(s)	161, 2644, 3004
Hpy166II	GTNNAC	7 cut(s)	73, 400, 443, 674, 1391, 1917, 2217
Hpy8I	GTNNAC	7 cut(s)	73, 400, 443, 674, 1391, 1917, 2217
HpyCH4IV	ACGT	6 cut(s)	147, 356, 445, 982, 2859, 2956
HpyF3I	CTNAG	5 cut(s)	1293, 1935, 1992, 2067, 3117
HpySE526I	ACGT	6 cut(s)	147, 356, 445, 982, 2859, 2956
HspAI	GCGC	4 cut(s)	48, 1044, 1258, 1423
KflI	GGGWCCC	1 cut(s)	950
Ksp22I	TGATCA	4 cut(s)	340, 972, 1957, 1984
LmnI	GCTCC	7 cut(s)	1034, 1306, 1714, 1804, 2050, 2617, 2779
MaeI	CTAG	5 cut(s)	182, 285, 483, 735, 750
MaeII	ACGT	6 cut(s)	147, 356, 445, 982, 2859, 2956
MfeI	CAATTG	3 cut(s)	270, 592, 3012
MflI	RGATCY	8 cut(s)	262, 608, 933, 1139, 1456, 1628, 2534, 3166
MhlI	GDGCHC	1 cut(s)	1711
MlsI	TGGCCA	2 cut(s)	232, 3230
MluNI	TGGCCA	2 cut(s)	232, 3230
MlyI	GAGTC	7 cut(s)	473, 679, 2245, 2532, 2891, 3157, 3199
MmeI	TCCRAC	3 cut(s)	1681, 2566, 2944
Mox20I	TGGCCA	2 cut(s)	232, 3230
Mph1103I	ATGCAT	1 cut(s)	2689
MroXI	GAANNNNTTC	3 cut(s)	264, 2743, 2911
MscI	TGGCCA	2 cut(s)	232, 3230
MseI	TTAA	5 cut(s)	1164, 1245, 1452, 2340, 3250
MslI	CAYNNNNRTG	3 cut(s)	174, 966, 1205
Msp20I	TGGCCA	2 cut(s)	232, 3230
MspA1I	CMGCKG	3 cut(s)	1655, 2714, 3002
MspI	CCGG	3 cut(s)	2616, 3189, 3238
MspR9I	CCNGG	8 cut(s)	414, 1626, 1649, 2099, 2616, 2940, 3232, 3239
MunI	CAATTG	3 cut(s)	270, 592, 3012
MvaI	CCWGG	6 cut(s)	414, 1626, 1649, 2099, 2940, 3232
MvnI	CGCG	1 cut(s)	1044
NciI	CCSGG	2 cut(s)	2616, 3239
NcoI	CCATGG	1 cut(s)	763
NdeI	CATATG	3 cut(s)	103, 1603, 1921
NlaIV	GGNNCC	8 cut(s)	488, 746, 870, 951, 952, 1710, 1800, 3236
NmeAIII	GCCGAG	1 cut(s)	2839
NmuCI	GTSAC	5 cut(s)	623, 635, 2237, 2350, 2650
NsiI	ATGCAT	1 cut(s)	2689
NspI	RCATGY	2 cut(s)	965, 1445
PaeR7I	CTCGAG	1 cut(s)	3176
PagI	TCATGA	1 cut(s)	343
PaqCI	CACCTGC	1 cut(s)	1124
PceI	AGGCCT	2 cut(s)	1884, 2488
PciI	ACATGT	1 cut(s)	961
PcsI	WCGNNNNNNNCGW	3 cut(s)	610, 619, 964
PdmI	GAANNNNTTC	3 cut(s)	264, 2743, 2911
PfeI	GAWTC	5 cut(s)	311, 557, 617, 2894, 2921
PflFI	GACNNNGTC	1 cut(s)	146
PflMI	CCANNNNNTGG	1 cut(s)	1808
PfoI	TCCNGGA	2 cut(s)	1624, 3237
PleI	GAGTC	7 cut(s)	473, 678, 2244, 2531, 2890, 3156, 3199
PpsI	GAGTC	7 cut(s)	473, 678, 2244, 2531, 2890, 3156, 3199
PpuMI	RGGWCCY	2 cut(s)	744, 950
PscI	ACATGT	1 cut(s)	961
PshAI	GACNNNNGTC	1 cut(s)	540
Psp5II	RGGWCCY	2 cut(s)	744, 950
Psp6I	CCWGG	6 cut(s)	412, 1624, 1647, 2097, 2938, 3230
PspGI	CCWGG	6 cut(s)	412, 1624, 1647, 2097, 2938, 3230
PspN4I	GGNNCC	8 cut(s)	488, 746, 870, 951, 952, 1710, 1800, 3236
PspPI	GGNCC	8 cut(s)	90, 486, 705, 744, 868, 950, 1364, 1622
PspPPI	RGGWCCY	2 cut(s)	744, 950
PstI	CTGCAG	5 cut(s)	1309, 1750, 2110, 2128, 2452
PstNI	CAGNNNCTG	2 cut(s)	1658, 2456
PsuI	RGATCY	8 cut(s)	262, 608, 933, 1139, 1456, 1628, 2534, 3166
PsyI	GACNNNGTC	1 cut(s)	146
PvuII	CAGCTG	3 cut(s)	1655, 2714, 3002
RsaI	GTAC	6 cut(s)	806, 1512, 1691, 1782, 1943, 2798
RsaNI	GTAC	6 cut(s)	805, 1511, 1690, 1781, 1942, 2797
RseI	CAYNNNNRTG	3 cut(s)	174, 966, 1205
SaqAI	TTAA	5 cut(s)	1164, 1245, 1452, 2340, 3250
Sau96I	GGNCC	8 cut(s)	90, 486, 705, 744, 868, 950, 1364, 1622
SchI	GAGTC	7 cut(s)	473, 679, 2245, 2532, 2891, 3157, 3199
ScrFI	CCNGG	8 cut(s)	414, 1626, 1649, 2099, 2616, 2940, 3232, 3239
SduI	GDGCHC	1 cut(s)	1711
SfcI	CTRYAG	5 cut(s)	1305, 1746, 2106, 2124, 2448
Sfr274I	CTCGAG	1 cut(s)	3176
SinI	GGWCC	5 cut(s)	705, 744, 950, 1364, 1622
SlaI	CTCGAG	1 cut(s)	3176
SmiMI	CAYNNNNRTG	3 cut(s)	174, 966, 1205
SmlI	CTYRAG	4 cut(s)	136, 238, 1571, 3176
SmoI	CTYRAG	4 cut(s)	136, 238, 1571, 3176
SseBI	AGGCCT	2 cut(s)	1884, 2488
SsiI	CCGC	2 cut(s)	1767, 3093
SspMI	CTAG	5 cut(s)	182, 285, 483, 735, 750
StuI	AGGCCT	2 cut(s)	1884, 2488
StyD4I	CCNGG	8 cut(s)	412, 1624, 1647, 2097, 2614, 2938, 3230, 3237
StyI	CCWWGG	4 cut(s)	763, 2001, 2483, 2869
TaiI	ACGT	6 cut(s)	150, 359, 448, 985, 2862, 2959
TaqI	TCGA	6 cut(s)	544, 757, 778, 958, 1104, 3177
TaqII	GACCGA	2 cut(s)	642, 693
TatI	WGTACW	3 cut(s)	1510, 1780, 1941
TfiI	GAWTC	5 cut(s)	311, 557, 617, 2894, 2921
Tru1I	TTAA	5 cut(s)	1164, 1245, 1452, 2340, 3250
Tru9I	TTAA	5 cut(s)	1164, 1245, 1452, 2340, 3250
TscAI	CASTG	7 cut(s)	1280, 1750, 1960, 2443, 2520, 3114, 3231
TseFI	GTSAC	5 cut(s)	623, 635, 2237, 2350, 2650
Tsp45I	GTSAC	5 cut(s)	623, 635, 2237, 2350, 2650
TspDTI	ATGAA	7 cut(s)	90, 141, 303, 1083, 1413, 2619, 2826
TspGWI	ACGGA	4 cut(s)	956, 2696, 3112, 3145
TspRI	CASTG	7 cut(s)	1280, 1750, 1960, 2443, 2520, 3114, 3231
Tth111I	GACNNNGTC	1 cut(s)	146
Van91I	CCANNNNNTGG	1 cut(s)	1808
VpaK11BI	GGWCC	5 cut(s)	705, 744, 950, 1364, 1622
XapI	RAATTY	4 cut(s)	374, 1156, 1478, 1587
XbaI	TCTAGA	1 cut(s)	181
XceI	RCATGY	2 cut(s)	965, 1445
XhoI	CTCGAG	1 cut(s)	3176
XmiI	GTMKAC	1 cut(s)	1916
XmnI	GAANNNNTTC	3 cut(s)	264, 2743, 2911
XspI	CTAG	5 cut(s)	182, 285, 483, 735, 750
Zsp2I	ATGCAT	1 cut(s)	2689

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.