Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0002501

Physical Location & Seq

Genomic Coordinates Reverse (-)

50056259 .. 50070902

Gene Structure 14644 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00321020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3051 bp

ATGAAGACGCAGCGAGATTGGAGGGTCGGTGGTTCATATGATGAGAGAAGGTTTATAGAGGAAAGGTACTCAAGGGACAATGTTTATCCAAGAAGCACATTCCACCGTGAAGTTCTGGATAGGGATAACTACCCACCACCACCTCATGCTGTTGGTATATGGCCAAATTCAAGAAGGAGAAGTTATGAAGAAGAATTTCCCATTGATAGGGAACCTAGGAGACATGAGAAACAGTACATGGATTCATATCATGAGATGGATAACCTCTGTGATCATGAGATTGATACATTTCAGGAATTTGATAAATTACGGGATGGCTATCACAGTATTGACAGCTATCGGGATCCTGGGTTTGAGAGGACTTCAAGGCTTGGGGGACGTGATCGTGATGATTATGCATATGATGATTTTGACTATAGGTCCGGTATTACCCATAAGACCAGGGGGGACAGTCGTGAAAGGGATTATGACTATGGTCGGCATAGTTATGATTCTGATTATGACAGAGGTAGTAGGAGAGAGGGCAATTGGAGGCGTCGTGAATCTCGTGACCGTGAGCGTGACAAGAAATGTTTAAGTAGGGAAAGAGAAATGAGTCCATATAGAAGGAATGAGCGTTCTAGATCTCGGTCTCGATCTCATGGGCATGATGATCATCCCAGATCAAGGTCACCTAGAAGTCGAAGCCATGGCCGAAGTCATCGAGAGGACAGCTATGATGATGGAAGGTATGAGAGAACTGATAAGCGAAGAGACAGGGATGAGAAACGCCAGAGAGAGCATTATTCTGTGGCCCCATCTGCAACTATTGTTGTGAAGGGTCTTTCACAGAAGACCACTGAGGAAGATCTATACCAGATCCTTGCTGAATGGGGACCTCTTCGTCATGTCCGTGTGATCAAAGAGCGTAATTCCGGCATTTCCCGTGGATTTGCTTTTATTGATTTCCCTTCCGTGGGAGCAGCTCGTATTATGATGGACAAACTCGGGGACAATGGGCATGTGGTGGATGGCAGGAAACTATTTTTTGAGTACAGTAGTAAGCCAACTGGGGGTGCAGGTGGATCTTTTGGTCAGGACAGTACTGGGAAATCTGGCCATGCTAACCATAAAAGCATCACTGTGCCATCTGATTGGATGTGTATCAGCTGTGGATATGTTAATTTTGCACGAAGAACATCCTGCTTTCAGTGTAATGAGGCACGAACTGAGGATGCTCCCGCAGCAGATATTTCTTCATCCAATCAAATGACAGGAAAGAAAGGATCAGAGGCTGGTCCTACTCATGTTTTGGTTGTTCGTGGACTGGATGAAAATGCTGATGAGGAAATGCTGCGCTATGAATTTTCCAAACATGCTCCTATCAAGGATCTACGTCTTGTCAGAGACAAATTCACTCATGTTTCACGGGGATTTGCGTTTGTACACTTCCATTCGGTGGAAGATGCTACAAAAGCTCTTGAAGCAACAAATGGAACACCACTTGAAAGGAATGGGCAGATTTTAAGAGTAGCATATGCAAAAAGCATTCTTGGTCCTGGATCAGGCATGTCAGGATCCTCACAGTCAAGCAGCCTGGCAGCTGCTGCAATTGAGGCAGCAACATTTGCTCAACAGTATGATGCTGTTGGATGGGCACCAAAGGAATATAATCCAGACGACAAACAATCCACGGGAGGGAAGGAGCAAAGTGGCGGGGATTTAAAAGCAGAAAAAGATGGTTTGGCTCCACAAACTGGTTTTGTATGGGATGAGGCATCCGGTTACTACTATGATGCTTCTTCCGGGTTCTACTATGATGGAAATACAGGTCTCTATTATGATGGTAACAATGGGATTTGGTATTCTTATGATCACCAAACTCTGCAGTACATCCCTTGCACTGATCAGAATGGCATCAAAGAATCTGCTAACCAATCTCAGCTTTCGAAGGCATCAGACGGTTCAAGTACCAAAAAAGTAGTCATCTCTGCACCAGCTACAACCTCAGCTGCATCATTACCAGATGCAGTCCAGGCTGCTGCAACAGCAGCGATAGCTGCAGAGAGAAAAGAAAAGGAAAAGTCCAAAGAGATCAAGTTGGCATCAAAGAGCAGTATTTTGGCTAACAAGAAGAAAATGAGTAATGTATTGACTATGTGGAAGCAGAGGAGTCATGAAGGGCAAGTAACCCGTGTAGCTCTAGATGATAACCAGCCAAGTGCACCTGCGAATGATAGACTGGTTTCTTCTGTTCCATCTACAAAGACAAAGTCTAAAATTGATGTACCAACCAAAAATGAGAATACTATATCTAGCTCAGGGGTTACCGCCAATGTTTCTATTGCAGATACTTGTGGTTTAGAGTCTTCAGTCATGCCAAGGCCTGTGGGCAACAGTATAGGAGGGACACTGATGGGAGTGATAAGGGGATCTGGAAGAACTGTAGTAAAATCAGATACATCCTTTTCAGCATCTGGTGGAGTTTCCGCACCTTCAACTTCTGCTGCATGCATAGCAGGTTCTTCTGCATCAACAGATTCGGATATCTCCACTGTTTTGACACCCTTCAGAACTGATGCATCGGCACTAGGTTCTTATACACCACCTGTGGCTGCTGGGAGTGGCAAGAGAAGATTCTCTGAAATGCCAGCTTCTGCTCATAAAGAACCTCATACTGGATACAGGGATCGTGCGGCTGAAAGGAGGAGCTTGTATGGCTCGTCATCTTCTTTTGGAGATGATTTGCCTGAACATGGTTTTGGGGAGTCAAGACGTGGGACTGGAGATGCAACCATTGACAGTTACGAGGTTATTACAGCCGACAAAGCAATTGATGAGAGCAATGTGGGCAACAAGATGCTTCGCAGCATGGGCTGGCACGAAGGCTTGGGTTTAGGAAGAGATGGGAGTGGAATGGTGGAACCAGTGCAGGCTCAGAGTGTAGAAAGGAGAGCCGGACTTGGGAGTCAGCAGAAGAAGTTAGATCCGGCTCTTGAGGCTCAGGCTGGAGATAGTTATAAAACTCTCATTCACAAGAAGGCACTGGCTAGGTTCCGGGAGATGTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000375 GO:0000377 GO:0000381 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0010468 GO:0010941 GO:0010942 GO:0016043 GO:0016070 GO:0016071 GO:0019219 GO:0019222 GO:0022607 GO:0022613 GO:0022618 GO:0031323 GO:0034622 GO:0034641 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043484 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0048024 GO:0048518 GO:0048522 GO:0050684 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065003 GO:0065007 GO:0071704 GO:0071826 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1903311

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

G-patch RRM_1

Protein Analysis

1016

Amino Acids

112.69

Weight (kDa)

8.27

Isoelectric Point (pI)

56.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_1	PF00076	270 - 340	1.6e-11	RNA recognition motif
RRM_1	PF00076	431 - 496	2.5e-17	RNA recognition motif
OCRE	PF17780	578 - 626	1.7e-17	OCRE domain
G-patch	PF01585	941 - 982	3.5e-14	G-patch domain
G-patch_2	PF12656	941 - 986	1.4e-06	G-patch domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002501)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54230 AT3G54230 AT3G54230 AT3G54230 AT3G54230
fragaria_vesca	FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410 FvH4_7g04410
malus_domestica	MD00G1152000.v1.1 MD02G1270000.v1.1
prunus_persica	Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1 Prupe.2G049800_v2.0.a1
pyrus_communis	pycom02g23120 pycom07g03700
rosa_chinensis	RchiOBHm_Chr1g0329501
rosa_laevigata	RLG00000029881
rosa_multiflora	Rmu_sc0004805.1_g000032 Rmu_sc0016170.1_g000004
rosa_roxburghii	Rroxscaffold_4G00321020
rosa_rugosa	Rorug01G0081000 Rorug01G0081100
rosa_samantha	Rh1AG099500 Rh1BG079200 Rh1CG095400 Rh1DG103200
rosa_wichuraiana	Rw0G014300 Rw1G007750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3000
AarI	CACCTGC	2 cut(s)	1049, 2215
AasI	GACNNNNNNGTC	2 cut(s)	882, 2946
Acc36I	ACCTGC	3 cut(s)	1049, 2215, 2489
AccB1I	GGYRCC	1 cut(s)	1636
AccB7I	CCANNNNNTGG	2 cut(s)	1438, 1736
AciI	CCGC	5 cut(s)	1221, 1695, 2310, 2469, 2675
AcoI	YGGCCR	3 cut(s)	161, 691, 1096
AcsI	RAATTY	5 cut(s)	166, 194, 296, 1343, 1391
AcuI	CTGAAG	2 cut(s)	2334, 2533
AcyI	GRCGYC	1 cut(s)	535
AfaI	GTAC	8 cut(s)	68, 236, 1034, 1084, 1425, 1871, 1951, 2268
AgsI	TTSAA	6 cut(s)	171, 366, 1463, 1487, 1947, 2478
AhdI	GACNNNNNGTC	1 cut(s)	418
AjiI	CACGTC	2 cut(s)	380, 2756
AjnI	CCWGG	5 cut(s)	346, 440, 1537, 1575, 2013
AjuI	GAANNNNNNNTTGG	2 cut(s)	2724, 2756
AloI	GAACNNNNNNTCC	2 cut(s)	601, 633
Alw21I	GWGCWC	1 cut(s)	2206
Alw26I	GTCTC	5 cut(s)	214, 636, 747, 1380, 1817
Alw44I	GTGCAC	1 cut(s)	2202
AlwNI	CAGNNNCTG	4 cut(s)	1274, 1586, 2456, 2636
Ama87I	CYCGRG	1 cut(s)	986
AoxI	GGCC	5 cut(s)	161, 691, 792, 1096, 2363
ApaLI	GTGCAC	1 cut(s)	2202
ApoI	RAATTY	5 cut(s)	166, 194, 296, 1343, 1391
ArsI	GACNNNNNNTTYG	4 cut(s)	1947, 1979, 2237, 2269
Asp700I	GAANNNNTTC	1 cut(s)	195
AspA2I	CCTAGG	1 cut(s)	215
AspLEI	GCGC	1 cut(s)	1338
AspS9I	GGNCC	5 cut(s)	420, 793, 875, 1277, 1535
AsuC2I	CCSGG	2 cut(s)	1786, 3038
AsuHPI	GGTGA	2 cut(s)	663, 1847
AsuII	TTCGAA	1 cut(s)	1928
AvaI	CYCGRG	1 cut(s)	986
AvaII	GGWCC	4 cut(s)	420, 875, 1277, 1535
AvrII	CCTAGG	1 cut(s)	215
BaeGI	GKGCMC	2 cut(s)	1639, 2206
BalI	TGGCCA	2 cut(s)	163, 1098
BamHI	GGATCC	2 cut(s)	343, 1556
BanI	GGYRCC	1 cut(s)	1636
BauI	CACGAG	1 cut(s)	546
BbsI	GAAGAC	3 cut(s)	11, 839, 2340
Bbv12I	GWGCWC	1 cut(s)	2206
BbvCI	CCTCAGC	1 cut(s)	1987
BciT130I	CCWGG	5 cut(s)	348, 442, 1539, 1577, 2015
BciVI	GTATCC	1 cut(s)	2654
BclI	TGATCA	5 cut(s)	271, 652, 897, 1852, 1885
BcnI	CCSGG	2 cut(s)	1786, 3038
BcoDI	GTCTC	5 cut(s)	214, 636, 747, 1380, 1817
BfaI	CTAG	7 cut(s)	216, 621, 675, 2183, 2295, 2570, 3030
BfmI	CTRYAG	4 cut(s)	415, 1865, 2040, 2424
BfuAI	ACCTGC	3 cut(s)	1049, 2215, 2489
BfuI	GTATCC	1 cut(s)	2654
BglII	AGATCT	2 cut(s)	623, 847
BlnI	CCTAGG	1 cut(s)	215
BmcAI	AGTACT	1 cut(s)	1084
Bme1390I	CCNGG	7 cut(s)	348, 442, 1539, 1577, 1786, 2015, 3038
Bme18I	GGWCC	4 cut(s)	420, 875, 1277, 1535
BmeRI	GACNNNNNGTC	1 cut(s)	418
BmeT110I	CYCGRG	1 cut(s)	986
BmgBI	CACGTC	2 cut(s)	380, 2756
BmgT120I	GGNCC	5 cut(s)	420, 793, 875, 1277, 1535
BmiI	GGNNCC	9 cut(s)	213, 345, 795, 876, 1558, 1638, 1728, 2904, 3035
BmrFI	CCNGG	7 cut(s)	348, 442, 1539, 1577, 1786, 2015, 3038
BmrI	ACTGGG	2 cut(s)	1059, 1095
BmuI	ACTGGG	2 cut(s)	1059, 1095
BoxI	GACNNNNGTC	1 cut(s)	474
BpiI	GAAGAC	3 cut(s)	11, 839, 2340
BpmI	CTGGAG	2 cut(s)	2784, 3009
Bpu10I	CCTNAGC	3 cut(s)	1987, 2299, 2982
Bpu14I	TTCGAA	1 cut(s)	1928
BpuEI	CTTGAG	2 cut(s)	55, 2996
BpuMI	CCSGG	2 cut(s)	1786, 3038
BsaBI	GATNNNNATC	4 cut(s)	246, 318, 654, 1142
BsaHI	GRCGYC	1 cut(s)	535
BsaI	GGTCTC	2 cut(s)	636, 1817
BsaJI	CCNNGG	8 cut(s)	215, 347, 441, 688, 925, 954, 1671, 2360
BsaWI	WCCGGW	2 cut(s)	422, 1760
BsaXI	ACNNNNNCTCC	4 cut(s)	13, 43, 523, 553
Bse1I	ACTGG	9 cut(s)	1054, 1090, 1311, 1741, 2226, 2662, 2767, 2906, 3030
Bse3DI	GCAATG	1 cut(s)	2830
Bse8I	GATNNNNATC	4 cut(s)	246, 318, 654, 1142
BseBI	CCWGG	5 cut(s)	348, 442, 1539, 1577, 2015
BseDI	CCNNGG	8 cut(s)	215, 347, 441, 688, 925, 954, 1671, 2360
BseJI	GATNNNNATC	4 cut(s)	246, 318, 654, 1142
BseMI	GCAATG	1 cut(s)	2830
BseMII	CTCAG	7 cut(s)	831, 1200, 1934, 2001, 2313, 2930, 2996
BseNI	ACTGG	9 cut(s)	1054, 1090, 1311, 1741, 2226, 2662, 2767, 2906, 3030
BseRI	GAGGAG	2 cut(s)	2164, 2701
BseSI	GKGCMC	2 cut(s)	1639, 2206
BseYI	CCCAGC	1 cut(s)	2597
BsgI	GTGCAG	3 cut(s)	1077, 1956, 2930
BshFI	GGCC	5 cut(s)	163, 693, 794, 1098, 2365
BshNI	GGYRCC	1 cut(s)	1636
BsiHKAI	GWGCWC	1 cut(s)	2206
BsiHKCI	CYCGRG	1 cut(s)	986
BsiSI	CCGG	7 cut(s)	423, 915, 1761, 1785, 2937, 2969, 3037
BslFI	GGGAC	7 cut(s)	89, 390, 461, 888, 1004, 2401, 2773
BsmAI	GTCTC	5 cut(s)	214, 636, 747, 1380, 1817
BsmFI	GGGAC	7 cut(s)	89, 390, 461, 888, 1004, 2401, 2773
BsmI	GAATGC	1 cut(s)	1527
BsnI	GGCC	5 cut(s)	163, 693, 794, 1098, 2365
Bso31I	GGTCTC	2 cut(s)	636, 1817
BsoBI	CYCGRG	1 cut(s)	986
Bsp119I	TTCGAA	1 cut(s)	1928
Bsp1286I	GDGCHC	2 cut(s)	1639, 2206
Bsp1407I	TGTACA	1 cut(s)	1423
Bsp19I	CCATGG	1 cut(s)	688
BspACI	CCGC	5 cut(s)	1221, 1695, 2310, 2469, 2675
BspANI	GGCC	5 cut(s)	163, 693, 794, 1098, 2365
BspCNI	CTCAG	7 cut(s)	832, 1201, 1933, 2000, 2312, 2929, 2995
BspHI	TCATGA	3 cut(s)	250, 274, 2155
BspLI	GGNNCC	9 cut(s)	213, 345, 795, 876, 1558, 1638, 1728, 2904, 3035
BspMAI	CTGCAG	2 cut(s)	1869, 2044
BspMI	ACCTGC	3 cut(s)	1049, 2215, 2489
BspT104I	TTCGAA	1 cut(s)	1928
BspT107I	GGYRCC	1 cut(s)	1636
BspTNI	GGTCTC	2 cut(s)	636, 1817
BsrDI	GCAATG	1 cut(s)	2830
BsrGI	TGTACA	1 cut(s)	1423
BsrI	ACTGG	9 cut(s)	1054, 1090, 1311, 1741, 2226, 2662, 2767, 2906, 3030
BssECI	CCNNGG	8 cut(s)	215, 347, 441, 688, 925, 954, 1671, 2360
BssNI	GRCGYC	1 cut(s)	535
BssSI	CACGAG	1 cut(s)	546
BssT1I	CCWWGG	3 cut(s)	215, 688, 2360
Bst2BI	CACGAG	1 cut(s)	546
Bst2UI	CCWGG	5 cut(s)	348, 442, 1539, 1577, 2015
Bst6I	CTCTTC	3 cut(s)	745, 885, 2875
BstACI	GRCGYC	1 cut(s)	535
BstAPI	GCANNNNNTGC	2 cut(s)	1586, 1607
BstAUI	TGTACA	1 cut(s)	1423
BstBI	TTCGAA	1 cut(s)	1928
BstC8I	GCNNGC	4 cut(s)	2491, 2631, 2858, 2913
BstDEI	CTNAG	7 cut(s)	840, 1209, 1920, 1987, 2299, 2916, 2982
BstDSI	CCRYGG	4 cut(s)	688, 925, 954, 1671
BstEII	GGTNACC	2 cut(s)	669, 2305
BstENI	CCTNNNNNAGG	1 cut(s)	1542
BstHHI	GCGC	1 cut(s)	1338
BstMAI	GTCTC	5 cut(s)	214, 636, 747, 1380, 1817
BstNI	CCWGG	5 cut(s)	348, 442, 1539, 1577, 2015
BstNSI	RCATGY	4 cut(s)	1004, 1358, 1552, 2493
BstPAI	GACNNNNGTC	1 cut(s)	474
BstPI	GGTNACC	2 cut(s)	669, 2305
BstSCI	CCNGG	7 cut(s)	346, 440, 1537, 1575, 1784, 2013, 3036
BstSFI	CTRYAG	4 cut(s)	415, 1865, 2040, 2424
BstSLI	GKGCMC	2 cut(s)	1639, 2206
BstV2I	GAAGAC	3 cut(s)	11, 839, 2340
BstX2I	RGATCY	9 cut(s)	343, 623, 847, 858, 1064, 1369, 1556, 2411, 2965
BstXI	CCANNNNNNTGG	1 cut(s)	1134
BstYI	RGATCY	9 cut(s)	343, 623, 847, 858, 1064, 1369, 1556, 2411, 2965
BsuI	GTATCC	1 cut(s)	2654
BsuRI	GGCC	5 cut(s)	163, 693, 794, 1098, 2365
BtgI	CCRYGG	4 cut(s)	688, 925, 954, 1671
BtrI	CACGTC	2 cut(s)	380, 2756
BtsIMutI	CAGTG	8 cut(s)	837, 1119, 1196, 1881, 2390, 2532, 2913, 3023
BveI	ACCTGC	3 cut(s)	1049, 2215, 2489
Cac8I	GCNNGC	4 cut(s)	2491, 2631, 2858, 2913
CaiI	CAGNNNCTG	4 cut(s)	1274, 1586, 2456, 2636
CciI	TCATGA	3 cut(s)	250, 274, 2155
CfoI	GCGC	1 cut(s)	1338
Cfr13I	GGNCC	5 cut(s)	420, 793, 875, 1277, 1535
CseI	GACGC	2 cut(s)	16, 524
Csp6I	GTAC	8 cut(s)	67, 235, 1033, 1083, 1424, 1870, 1950, 2267
CspCI	CAANNNNNGTGG	2 cut(s)	2349, 2384
CviQI	GTAC	8 cut(s)	67, 235, 1033, 1083, 1424, 1870, 1950, 2267
DdeI	CTNAG	7 cut(s)	840, 1209, 1920, 1987, 2299, 2916, 2982
DraI	TTTAAA	1 cut(s)	1704
DrdI	GACNNNNNNGTC	2 cut(s)	882, 2946
DriI	GACNNNNNGTC	1 cut(s)	418
DseDI	GACNNNNNNGTC	2 cut(s)	882, 2946
EaeI	YGGCCR	3 cut(s)	161, 691, 1096
Eam1104I	CTCTTC	3 cut(s)	745, 885, 2875
Eam1105I	GACNNNNNGTC	1 cut(s)	418
EarI	CTCTTC	3 cut(s)	745, 885, 2875
Eco130I	CCWWGG	3 cut(s)	215, 688, 2360
Eco147I	AGGCCT	1 cut(s)	2365
Eco31I	GGTCTC	2 cut(s)	636, 1817
Eco32I	GATATC	1 cut(s)	2527
Eco47I	GGWCC	4 cut(s)	420, 875, 1277, 1535
Eco57I	CTGAAG	2 cut(s)	2334, 2533
Eco88I	CYCGRG	1 cut(s)	986
Eco91I	GGTNACC	2 cut(s)	669, 2305
EcoNI	CCTNNNNNAGG	1 cut(s)	1542
EcoO109I	RGGNCCY	1 cut(s)	875
EcoO65I	GGTNACC	2 cut(s)	669, 2305
EcoRII	CCWGG	5 cut(s)	346, 440, 1537, 1575, 2013
EcoRV	GATATC	1 cut(s)	2527
EcoT14I	CCWWGG	3 cut(s)	215, 688, 2360
EcoT22I	ATGCAT	3 cut(s)	400, 2495, 2563
ErhI	CCWWGG	3 cut(s)	215, 688, 2360
FaqI	GGGAC	7 cut(s)	89, 390, 461, 888, 1004, 2401, 2773
FauI	CCCGC	2 cut(s)	1228, 1688
FauNDI	CATATG	3 cut(s)	37, 400, 1516
FbaI	TGATCA	5 cut(s)	271, 652, 897, 1852, 1885
FspBI	CTAG	7 cut(s)	216, 621, 675, 2183, 2295, 2570, 3030
GlaI	GCGC	1 cut(s)	1337
GsaI	CCCAGC	1 cut(s)	2601
GsuI	CTGGAG	2 cut(s)	2784, 3009
HaeIII	GGCC	5 cut(s)	163, 693, 794, 1098, 2365
HapII	CCGG	7 cut(s)	423, 915, 1761, 1785, 2937, 2969, 3037
HgaI	GACGC	2 cut(s)	16, 524
HhaI	GCGC	1 cut(s)	1338
Hin1I	GRCGYC	1 cut(s)	535
Hin6I	GCGC	1 cut(s)	1336
HinP1I	GCGC	1 cut(s)	1336
HpaII	CCGG	7 cut(s)	423, 915, 1761, 1785, 2937, 2969, 3037
HphI	GGTGA	2 cut(s)	663, 1847
Hpy166II	GTNNAC	3 cut(s)	1304, 1426, 2204
Hpy8I	GTNNAC	3 cut(s)	1304, 1426, 2204
Hpy99I	CGWCG	1 cut(s)	540
HpyCH4IV	ACGT	3 cut(s)	379, 1375, 2755
HpyF3I	CTNAG	7 cut(s)	840, 1209, 1920, 1987, 2299, 2916, 2982
HpySE526I	ACGT	3 cut(s)	379, 1375, 2755
Hsp92I	GRCGYC	1 cut(s)	535
HspAI	GCGC	1 cut(s)	1336
Ksp22I	TGATCA	5 cut(s)	271, 652, 897, 1852, 1885
LmnI	GCTCC	6 cut(s)	959, 1222, 1363, 1684, 1732, 2688
MaeI	CTAG	7 cut(s)	216, 621, 675, 2183, 2295, 2570, 3030
MaeII	ACGT	3 cut(s)	379, 1375, 2755
MaeIII	GTNAC	8 cut(s)	548, 560, 669, 1763, 1826, 2167, 2305, 2783
MfeI	CAATTG	3 cut(s)	526, 1590, 2811
MflI	RGATCY	9 cut(s)	343, 623, 847, 858, 1064, 1369, 1556, 2411, 2965
MhlI	GDGCHC	2 cut(s)	1639, 2206
MlsI	TGGCCA	2 cut(s)	163, 1098
MluNI	TGGCCA	2 cut(s)	163, 1098
MlyI	GAGTC	5 cut(s)	604, 2161, 2354, 2756, 2956
MmeI	TCCRAC	1 cut(s)	1609
Mox20I	TGGCCA	2 cut(s)	163, 1098
Mph1103I	ATGCAT	3 cut(s)	400, 2495, 2563
MroXI	GAANNNNTTC	1 cut(s)	195
MscI	TGGCCA	2 cut(s)	163, 1098
MseI	TTAA	4 cut(s)	575, 1161, 1505, 1703
MslI	CAYNNNNRTG	4 cut(s)	486, 645, 891, 1121
Msp20I	TGGCCA	2 cut(s)	163, 1098
MspA1I	CMGCKG	3 cut(s)	1149, 1583, 1991
MspI	CCGG	7 cut(s)	423, 915, 1761, 1785, 2937, 2969, 3037
MspR9I	CCNGG	7 cut(s)	348, 442, 1539, 1577, 1786, 2015, 3038
MunI	CAATTG	3 cut(s)	526, 1590, 2811
Mva1269I	GAATGC	1 cut(s)	1527
MvaI	CCWGG	5 cut(s)	348, 442, 1539, 1577, 2015
NciI	CCSGG	2 cut(s)	1786, 3038
NcoI	CCATGG	1 cut(s)	688
NdeI	CATATG	3 cut(s)	37, 400, 1516
NlaIV	GGNNCC	9 cut(s)	213, 345, 795, 876, 1558, 1638, 1728, 2904, 3035
NmuCI	GTSAC	3 cut(s)	548, 560, 669
NsiI	ATGCAT	3 cut(s)	400, 2495, 2563
NspI	RCATGY	4 cut(s)	1004, 1358, 1552, 2493
NspV	TTCGAA	1 cut(s)	1928
PaeI	GCATGC	1 cut(s)	2493
PagI	TCATGA	3 cut(s)	250, 274, 2155
PaqCI	CACCTGC	2 cut(s)	1049, 2215
PceI	AGGCCT	1 cut(s)	2365
PcsI	WCGNNNNNNNCGW	2 cut(s)	544, 889
PctI	GAATGC	1 cut(s)	1527
PdmI	GAANNNNTTC	1 cut(s)	195
PfeI	GAWTC	6 cut(s)	242, 491, 542, 1904, 2519, 2616
PflFI	GACNNNGTC	1 cut(s)	2251
PflMI	CCANNNNNTGG	2 cut(s)	1438, 1736
PfoI	TCCNGGA	2 cut(s)	1537, 3036
PleI	GAGTC	5 cut(s)	603, 2160, 2353, 2755, 2955
PpsI	GAGTC	5 cut(s)	603, 2160, 2353, 2755, 2955
PpuMI	RGGWCCY	1 cut(s)	875
PshAI	GACNNNNGTC	1 cut(s)	474
PsiI	TTATAA	1 cut(s)	3000
Psp5II	RGGWCCY	1 cut(s)	875
Psp6I	CCWGG	5 cut(s)	346, 440, 1537, 1575, 2013
PspEI	GGTNACC	2 cut(s)	669, 2305
PspFI	CCCAGC	1 cut(s)	2597
PspGI	CCWGG	5 cut(s)	346, 440, 1537, 1575, 2013
PspN4I	GGNNCC	9 cut(s)	213, 345, 795, 876, 1558, 1638, 1728, 2904, 3035
PspPI	GGNCC	5 cut(s)	420, 793, 875, 1277, 1535
PspPPI	RGGWCCY	1 cut(s)	875
PstI	CTGCAG	2 cut(s)	1869, 2044
PstNI	CAGNNNCTG	4 cut(s)	1274, 1586, 2456, 2636
PsuI	RGATCY	9 cut(s)	343, 623, 847, 858, 1064, 1369, 1556, 2411, 2965
PsyI	GACNNNGTC	1 cut(s)	2251
PvuII	CAGCTG	3 cut(s)	1149, 1583, 1991
RsaI	GTAC	8 cut(s)	68, 236, 1034, 1084, 1425, 1871, 1951, 2268
RsaNI	GTAC	8 cut(s)	67, 235, 1033, 1083, 1424, 1870, 1950, 2267
RseI	CAYNNNNRTG	4 cut(s)	486, 645, 891, 1121
SaqAI	TTAA	4 cut(s)	575, 1161, 1505, 1703
Sau96I	GGNCC	5 cut(s)	420, 793, 875, 1277, 1535
ScaI	AGTACT	1 cut(s)	1084
SchI	GAGTC	5 cut(s)	604, 2161, 2354, 2756, 2956
ScrFI	CCNGG	7 cut(s)	348, 442, 1539, 1577, 1786, 2015, 3038
SduI	GDGCHC	2 cut(s)	1639, 2206
SfcI	CTRYAG	4 cut(s)	415, 1865, 2040, 2424
SfuI	TTCGAA	1 cut(s)	1928
SinI	GGWCC	4 cut(s)	420, 875, 1277, 1535
SmiMI	CAYNNNNRTG	4 cut(s)	486, 645, 891, 1121
SmlI	CTYRAG	2 cut(s)	70, 2975
SmoI	CTYRAG	2 cut(s)	70, 2975
SphI	GCATGC	1 cut(s)	2493
SseBI	AGGCCT	1 cut(s)	2365
SsiI	CCGC	5 cut(s)	1221, 1695, 2310, 2469, 2675
SspMI	CTAG	7 cut(s)	216, 621, 675, 2183, 2295, 2570, 3030
StuI	AGGCCT	1 cut(s)	2365
StyD4I	CCNGG	7 cut(s)	346, 440, 1537, 1575, 1784, 2013, 3036
StyI	CCWWGG	3 cut(s)	215, 688, 2360
TaiI	ACGT	3 cut(s)	382, 1378, 2758
TaqI	TCGA	4 cut(s)	634, 682, 703, 1928
TaqII	GACCGA	1 cut(s)	618
TatI	WGTACW	5 cut(s)	234, 1032, 1082, 1423, 1869
TauI	GCSGC	1 cut(s)	2678
TfiI	GAWTC	6 cut(s)	242, 491, 542, 1904, 2519, 2616
Tru1I	TTAA	4 cut(s)	575, 1161, 1505, 1703
Tru9I	TTAA	4 cut(s)	575, 1161, 1505, 1703
TscAI	CASTG	8 cut(s)	844, 1126, 1196, 1888, 2397, 2539, 2913, 3030
TseFI	GTSAC	3 cut(s)	548, 560, 669
Tsp45I	GTSAC	3 cut(s)	548, 560, 669
TspDTI	ATGAA	8 cut(s)	17, 24, 201, 234, 1227, 1326, 1356, 2172
TspGWI	ACGGA	2 cut(s)	881, 943
TspRI	CASTG	8 cut(s)	844, 1126, 1196, 1888, 2397, 2539, 2913, 3030
Tth111I	GACNNNGTC	1 cut(s)	2251
Van91I	CCANNNNNTGG	2 cut(s)	1438, 1736
VneI	GTGCAC	1 cut(s)	2202
VpaK11BI	GGWCC	4 cut(s)	420, 875, 1277, 1535
XagI	CCTNNNNNAGG	1 cut(s)	1542
XapI	RAATTY	5 cut(s)	166, 194, 296, 1343, 1391
XbaI	TCTAGA	2 cut(s)	620, 2182
XceI	RCATGY	4 cut(s)	1004, 1358, 1552, 2493
XmaJI	CCTAGG	1 cut(s)	215
XmnI	GAANNNNTTC	1 cut(s)	195
XspI	CTAG	7 cut(s)	216, 621, 675, 2183, 2295, 2570, 3030
ZrmI	AGTACT	1 cut(s)	1084
Zsp2I	ATGCAT	3 cut(s)	400, 2495, 2563

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.